PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9237106-6 1997 IL-5 expression induced by either IL-2 or PHA was completely abolished by the protein synthesis inhibitor cycloheximide. Cycloheximide 106-119 interleukin 2 Homo sapiens 34-38 27077371-5 2016 Using cycloheximide, a general inhibitor of mRNA translation, we determined that Foxp3, but not CD25, has a high turnover in IL-2 stimulated Treg. Cycloheximide 6-19 interleukin 2 Homo sapiens 125-129 19428567-6 2009 Addition of cycloheximide indicated that the IL-2 effects were independent of de novo protein synthesis and that the HIC gene is a direct target of IL-2. Cycloheximide 12-25 interleukin 2 Homo sapiens 45-49 10397172-5 1999 This block in IL-2 mRNA splicing is relieved by the translation inhibitor, cycloheximide (CHX), which does not stimulate transcription [Gerez et al., J. Biol. Cycloheximide 75-88 interleukin 2 Homo sapiens 14-18 10397172-5 1999 This block in IL-2 mRNA splicing is relieved by the translation inhibitor, cycloheximide (CHX), which does not stimulate transcription [Gerez et al., J. Biol. Cycloheximide 90-93 interleukin 2 Homo sapiens 14-18 10397172-8 1999 We show that suppression of IL-2 mRNA expression, whether by CD8 cells, soluble mediators derived from them, or IL-10, is relieved completely by CHX. Cycloheximide 145-148 interleukin 2 Homo sapiens 28-32 7930567-7 1994 However, the induction of NGFI-B by IL-2 is dependent on the presence of cycloheximide. Cycloheximide 73-86 interleukin 2 Homo sapiens 36-40 7642643-6 1995 When translation is blocked (e.g. by cycloheximide), expression of IL-2 mRNA can be superinduced by 2 orders of magnitude. Cycloheximide 37-50 interleukin 2 Homo sapiens 67-71 7860090-8 1994 This 2.8-kb transcript was in addition to the expected 1.0-kb, transiently expressed IL-2 message, and it could be superinduced in the presence of cycloheximide. Cycloheximide 147-160 interleukin 2 Homo sapiens 85-89 7512581-2 1994 We show that the SP-augmented IL-2 mRNA signal is totally abrogated by an early addition of cyclosporin A, actinomycin D or cycloheximide. Cycloheximide 124-137 interleukin 2 Homo sapiens 30-34 8104219-6 1993 The anti-Kp43-mediated effect, which required the preactivation of cells with IL-2, was inhibited by cycloheximide and actinomycin D and was associated with an increase in the levels of TNF-alpha-specific mRNA. Cycloheximide 101-114 interleukin 2 Homo sapiens 78-82 7518260-6 1994 Addition of cycloheximide (CHx) 4 h after T cell activation resulted in substantial superinduction of IL-2 mRNA in the CD4+CD45RO+ population but had little effect on CD4+CD45RA+ cells. Cycloheximide 12-25 interleukin 2 Homo sapiens 102-106 7518260-6 1994 Addition of cycloheximide (CHx) 4 h after T cell activation resulted in substantial superinduction of IL-2 mRNA in the CD4+CD45RO+ population but had little effect on CD4+CD45RA+ cells. Cycloheximide 27-30 interleukin 2 Homo sapiens 102-106 7681987-3 1993 To increase the frequency of IL-2-induced transcripts represented in the library, the protein synthesis inhibitor cycloheximide was included during the 2-hr IL-2 stimulation to superinduce gene expression, and the uridine analogue 4-thiouridine was utilized to enable selective purification of newly synthesized transcripts. Cycloheximide 114-127 interleukin 2 Homo sapiens 29-33 7681987-3 1993 To increase the frequency of IL-2-induced transcripts represented in the library, the protein synthesis inhibitor cycloheximide was included during the 2-hr IL-2 stimulation to superinduce gene expression, and the uridine analogue 4-thiouridine was utilized to enable selective purification of newly synthesized transcripts. Cycloheximide 114-127 interleukin 2 Homo sapiens 157-161 1734947-11 1992 Moreover, cycloheximide treatment abolished the protective effect against NK-CMC induced by IFN-alpha or by IL-2. Cycloheximide 10-23 interleukin 2 Homo sapiens 108-112 1942774-3 1991 The latter was achieved by analyzing the superinduction of IL-2 and IFN-gamma mRNA occurring upon culture with cycloheximide or after low-dose gamma-irradiation, respectively. Cycloheximide 111-124 interleukin 2 Homo sapiens 59-63 1824688-3 1991 The latter was achieved by analyzing, respectively, the superinduction of IL-2 and IFN-gamma mRNA occurring upon culture with cycloheximide or after low-dose gamma-irradiation. Cycloheximide 126-139 interleukin 2 Homo sapiens 74-78 2033254-0 1991 Superinduction of IL-2 gene transcription in the presence of cycloheximide. Cycloheximide 61-74 interleukin 2 Homo sapiens 18-22 2033254-2 1991 To date, it has been shown that the protein synthesis inhibitor cycloheximide (CHX) up-regulates IL-2 expression in T cells by stabilizing its mRNA. Cycloheximide 64-77 interleukin 2 Homo sapiens 97-101 2033254-2 1991 To date, it has been shown that the protein synthesis inhibitor cycloheximide (CHX) up-regulates IL-2 expression in T cells by stabilizing its mRNA. Cycloheximide 79-82 interleukin 2 Homo sapiens 97-101 2531899-1 1989 In this report we present evidence that Con A-activated T helper 2 cells transcribe the interleukin 2 (IL-2) gene when cycloheximide is added 3 hr after stimulation. Cycloheximide 119-132 interleukin 2 Homo sapiens 88-101 2594781-5 1989 After 20 hr of culture, IL-2R mRNA was markedly decreased in both IL-2-toxin- and cycloheximide-treated phytohemagglutinin-activated T cells. Cycloheximide 82-95 interleukin 2 Homo sapiens 24-28 2594781-6 1989 Although interaction of IL-2-toxin with IL-2R+ T cells initially mimics the stimulatory effects of IL-2 upon c-myc, interferon gamma, IL-2R, and IL-2 gene expression, the consequences of inhibition of protein synthesis mediated by the ADP-ribosyltransferase activity of the toxin dominate after 7 hr and are indistinguishable from those effects mediated by cycloheximide. Cycloheximide 357-370 interleukin 2 Homo sapiens 24-28 2594781-6 1989 Although interaction of IL-2-toxin with IL-2R+ T cells initially mimics the stimulatory effects of IL-2 upon c-myc, interferon gamma, IL-2R, and IL-2 gene expression, the consequences of inhibition of protein synthesis mediated by the ADP-ribosyltransferase activity of the toxin dominate after 7 hr and are indistinguishable from those effects mediated by cycloheximide. Cycloheximide 357-370 interleukin 2 Homo sapiens 40-44 2594781-6 1989 Although interaction of IL-2-toxin with IL-2R+ T cells initially mimics the stimulatory effects of IL-2 upon c-myc, interferon gamma, IL-2R, and IL-2 gene expression, the consequences of inhibition of protein synthesis mediated by the ADP-ribosyltransferase activity of the toxin dominate after 7 hr and are indistinguishable from those effects mediated by cycloheximide. Cycloheximide 357-370 interleukin 2 Homo sapiens 40-44 2531899-1 1989 In this report we present evidence that Con A-activated T helper 2 cells transcribe the interleukin 2 (IL-2) gene when cycloheximide is added 3 hr after stimulation. Cycloheximide 119-132 interleukin 2 Homo sapiens 103-107 2531899-5 1989 Nuclear run-on experiments indicate that IL-2 transcription in T helper 2 cells depends on the presence of cycloheximide. Cycloheximide 107-120 interleukin 2 Homo sapiens 41-45 2786549-9 1989 The acquisition of cell resistance to perforin by these IL-2-independent cell lines after activation with stimulatory reagents was independent of protein and RNA neosynthesis: emetine, cycloheximide, and actinomycin D, while effectively blocking the incorporation of [35S]methionine into cell proteins, did not affect the induced increase in perforin resistance. Cycloheximide 185-198 interleukin 2 Homo sapiens 56-60 3258332-4 1988 The degradation of IL-2 mRNA is selectively sensitive to cycloheximide and actinomycin D, inhibitors of protein and RNA synthesis, respectively. Cycloheximide 57-70 interleukin 2 Homo sapiens 19-23 3139787-5 1988 Stimulation of IFN-gamma mRNA by both PMA and IL2 could occur in the presence of cycloheximide, indicating that protein synthesis was not required for the initial stimulation to occur. Cycloheximide 81-94 interleukin 2 Homo sapiens 46-49 3012540-8 1986 Addition of the protein synthesis inhibitor cycloheximide, before the addition of PHA to cultures, abolished the PHA-induced accumulation of mRNAs for c-myb, N-ras, and TFR, but not of mRNAs for c-fos, c-myc, IL2, and IL2R. Cycloheximide 44-57 interleukin 2 Homo sapiens 209-212 3096988-5 1986 The half-life for surface high affinity IL2 receptors, as measured in the presence of cycloheximide, is about 1 h and does not depend upon the presence of IL2. Cycloheximide 86-99 interleukin 2 Homo sapiens 40-43 3484763-7 1986 IL 2 mRNA could be superinduced several folds by addition of cycloheximide 3 hr after induction of J32 with mitogens. Cycloheximide 61-74 interleukin 2 Homo sapiens 0-4 2419474-11 1986 The subsequent downregulation of lymphokine and c-myc mRNAs was retarded by cycloheximide. Cycloheximide 76-89 interleukin 2 Homo sapiens 33-43 2983318-3 1985 In the presence of cycloheximide, IL-2 binding sites declined with a half-time (t1/2) of 2.6 hr, whereas the decay of anti-Tac binding sites was much slower (t 1/2 = 6.4 hr). Cycloheximide 19-32 interleukin 2 Homo sapiens 34-38 6333239-2 1984 We have previously demonstrated a dramatic superinduction of the formation of IL-2 and of biologically active IL-2 mRNA in the presence of cycloheximide, an inhibitor of translation. Cycloheximide 139-152 interleukin 2 Homo sapiens 78-82 6333239-2 1984 We have previously demonstrated a dramatic superinduction of the formation of IL-2 and of biologically active IL-2 mRNA in the presence of cycloheximide, an inhibitor of translation. Cycloheximide 139-152 interleukin 2 Homo sapiens 110-114 6609365-6 1984 The presence of cycloheximide leads to extensive superinduction of IL-2, concomitant with an increase in active IL-2 mRNA formation up to 30-fold over normal levels. Cycloheximide 16-29 interleukin 2 Homo sapiens 67-71 6609365-6 1984 The presence of cycloheximide leads to extensive superinduction of IL-2, concomitant with an increase in active IL-2 mRNA formation up to 30-fold over normal levels. Cycloheximide 16-29 interleukin 2 Homo sapiens 112-116 6609365-7 1984 The repressor appears to be short-lived, as the addition of cycloheximide after shutoff leads to an immediate resumption of active IL-2 mRNA formation. Cycloheximide 60-73 interleukin 2 Homo sapiens 131-135