PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 29467593-8 2018 Caspase activation was assessed by MTT and PI after treatments with Z-VAD [OME]-FMK, mitomycin c and cycloheximide. Cycloheximide 101-114 caspase 8 Homo sapiens 0-7 30902881-6 2019 Importantly, the loss of cIAP1 enhanced TNF-alpha/cycloheximide-induced apoptosis in higher activation statuses of Caspase-8, Caspase-3 without the induction of Complex II. Cycloheximide 50-63 caspase 8 Homo sapiens 115-124 29467593-14 2018 Subsequent activation of caspase-8 after co-incubation of mitomycin c and cycloheximide separately, restored the cell viability in cholesterol depleted MDA-MB 231 cells. Cycloheximide 74-87 caspase 8 Homo sapiens 25-34 25513960-4 2015 Treatment of MKN28 cells with TNF-alpha plus CHX induced degradation of survivin and activation of caspase-8 and -3, followed by degradation of cIAP1 and XIAP and apoptosis. Cycloheximide 45-48 caspase 8 Homo sapiens 99-115 12869656-7 2003 Both C2-ceramide and TNFalpha + CHX increased caspase 8- and 3-like activities in cytosolic extracts; however, treatment of cells with the broad-spectrum caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone protected NB16 cells from TNFalpha + CHX-induced cell death but did not prevent C2-ceramide cytotoxicity. Cycloheximide 32-35 caspase 8 Homo sapiens 46-55 23397952-6 2013 Cells were treated with TNF-alpha alone or in the presence of cycloheximide (CHX), which promotes caspase-8 activation by eliminating the endogenous caspase-8 inhibitor, c-FLIP. Cycloheximide 62-75 caspase 8 Homo sapiens 98-107 23397952-6 2013 Cells were treated with TNF-alpha alone or in the presence of cycloheximide (CHX), which promotes caspase-8 activation by eliminating the endogenous caspase-8 inhibitor, c-FLIP. Cycloheximide 62-75 caspase 8 Homo sapiens 149-158 23397952-6 2013 Cells were treated with TNF-alpha alone or in the presence of cycloheximide (CHX), which promotes caspase-8 activation by eliminating the endogenous caspase-8 inhibitor, c-FLIP. Cycloheximide 77-80 caspase 8 Homo sapiens 98-107 23397952-6 2013 Cells were treated with TNF-alpha alone or in the presence of cycloheximide (CHX), which promotes caspase-8 activation by eliminating the endogenous caspase-8 inhibitor, c-FLIP. Cycloheximide 77-80 caspase 8 Homo sapiens 149-158 18522940-4 2008 Caspase-8 on average was activated 45-600 min after TRAIL/cycloheximide addition. Cycloheximide 58-71 caspase 8 Homo sapiens 0-9 16888780-5 2007 In the presence of cycloheximide, the selected SCCHN sublines become susceptible to CH-11 Ab, and showed cleavage of caspase-8, suggesting that apoptosis resistance was mediated by an inhibitory protein(s) acting upstream of caspase-8. Cycloheximide 19-32 caspase 8 Homo sapiens 117-126 16888780-5 2007 In the presence of cycloheximide, the selected SCCHN sublines become susceptible to CH-11 Ab, and showed cleavage of caspase-8, suggesting that apoptosis resistance was mediated by an inhibitory protein(s) acting upstream of caspase-8. Cycloheximide 19-32 caspase 8 Homo sapiens 225-234 16133866-8 2005 The most striking anti-apoptotic effect though was obtained by the translational inhibitor cycloheximide, which abolished caspase 8 processing, blocked Bid cleavage and maintained the mitochondrial transmembrane potential. Cycloheximide 91-104 caspase 8 Homo sapiens 122-131 15094781-6 2004 TRAIL-induced cell death could be further enhanced by cotreatment of IGR-N91-C8 and SH-EP cells with cycloheximide or subtoxic concentrations of chemotherapeutic drugs in a caspase-dependent manner. Cycloheximide 101-114 caspase 8 Homo sapiens 173-180 14688367-11 2004 FLIP-caspase-8 balance seems tightly regulated in fibroblasts by extracellular factors that determine their susceptibility to Fas- or Fas-CHX-induced apoptosis. Cycloheximide 138-141 caspase 8 Homo sapiens 5-14 14632785-10 2003 Interestingly, in some cell lines, TRAIL sensitivity and caspase-8 activity was enhanced or restored with the treatment of cycloheximide (CHX). Cycloheximide 123-136 caspase 8 Homo sapiens 57-66 14632785-10 2003 Interestingly, in some cell lines, TRAIL sensitivity and caspase-8 activity was enhanced or restored with the treatment of cycloheximide (CHX). Cycloheximide 138-141 caspase 8 Homo sapiens 57-66 12938225-3 2003 Furthermore, following overexpression of a human Fas:FLICE construct, which directly induces caspase activation in a death-inducing signaling complex-independent manner, cells could not be protected through BCR stimulation.Co-incubation with cycloheximide partially reversed protection from apoptosis and increased Fas-stimulated initiator and effector caspase activation, suggesting new protein synthesis is necessary to induce protection upstream of caspase activation. Cycloheximide 242-255 caspase 8 Homo sapiens 53-58 18485876-2 2008 Cycloheximide promotes caspase-8 activation by eliminating endogenous caspase-8 inhibitor, c-FLIP, while Smac mimetic does so by triggering autodegradation of cIAP1 and cIAP2 (cIAP1/2), leading to the release of receptor interacting protein kinase (RIPK1) from the activated TNF receptor complex to form a caspase-8-activating complex consisting of RIPK1, FADD, and caspase-8. Cycloheximide 0-13 caspase 8 Homo sapiens 23-32 18485876-2 2008 Cycloheximide promotes caspase-8 activation by eliminating endogenous caspase-8 inhibitor, c-FLIP, while Smac mimetic does so by triggering autodegradation of cIAP1 and cIAP2 (cIAP1/2), leading to the release of receptor interacting protein kinase (RIPK1) from the activated TNF receptor complex to form a caspase-8-activating complex consisting of RIPK1, FADD, and caspase-8. Cycloheximide 0-13 caspase 8 Homo sapiens 70-79 18485876-2 2008 Cycloheximide promotes caspase-8 activation by eliminating endogenous caspase-8 inhibitor, c-FLIP, while Smac mimetic does so by triggering autodegradation of cIAP1 and cIAP2 (cIAP1/2), leading to the release of receptor interacting protein kinase (RIPK1) from the activated TNF receptor complex to form a caspase-8-activating complex consisting of RIPK1, FADD, and caspase-8. Cycloheximide 0-13 caspase 8 Homo sapiens 70-79 18485876-2 2008 Cycloheximide promotes caspase-8 activation by eliminating endogenous caspase-8 inhibitor, c-FLIP, while Smac mimetic does so by triggering autodegradation of cIAP1 and cIAP2 (cIAP1/2), leading to the release of receptor interacting protein kinase (RIPK1) from the activated TNF receptor complex to form a caspase-8-activating complex consisting of RIPK1, FADD, and caspase-8. Cycloheximide 0-13 caspase 8 Homo sapiens 70-79 18289527-3 2008 Nevertheless, we have surprisingly found that CHX, as well as its structural analogue acetoxycycloheximide (Ac-CHX), prevents TNF-alpha-mediated activation of NF-kappaB and caspase-8 in human lung carcinoma A549 cells. Cycloheximide 46-49 caspase 8 Homo sapiens 173-182 16478887-6 2006 Plasminogen treatment also markedly reduced internucleosomal DNA fragmentation and reduced levels of active caspase 3, caspase 8, and caspase 9 induced by TNFalpha or by cycloheximide. Cycloheximide 170-183 caspase 8 Homo sapiens 119-128 15924153-7 2005 Interestingly, cotreatment of Hank-1 with cycloheximide, a protein synthesis inhibitor, markedly sensitized cells to Fas-mediated apoptosis along with caspase 8 activation and c-FLIP(L) (cellular FLICE inhibitory protein long form) downregulation. Cycloheximide 42-55 caspase 8 Homo sapiens 151-160 15614529-2 2005 In contrast to a previous study, a rapid and dramatic decrease in levels of cellular FLICE (Fas-associated death domain-like IL-1beta-converting enzyme) inhibitory protein (cFLIP) following cycloheximide treatment was observed in all RCCs studied. Cycloheximide 190-203 caspase 8 Homo sapiens 85-90 15614529-2 2005 In contrast to a previous study, a rapid and dramatic decrease in levels of cellular FLICE (Fas-associated death domain-like IL-1beta-converting enzyme) inhibitory protein (cFLIP) following cycloheximide treatment was observed in all RCCs studied. Cycloheximide 190-203 caspase 8 Homo sapiens 92-151 15614529-5 2005 Therefore, cycloheximide treatment resulted in an increase in the pro-caspase-8 to cFLIP ratio, which correlated with sensitization to TRAIL-mediated apoptosis. Cycloheximide 11-24 caspase 8 Homo sapiens 70-79 11779361-5 2002 Cycloheximide also inhibited the activation of caspases and AP-1, the expression of Fas, the formation of DISC and the release of cytochrome-C, but not the activation of SAPK/JNK in X-irradiated MOLT-4 cells. Cycloheximide 0-13 caspase 8 Homo sapiens 47-55 12663669-4 2003 Cycloheximide but not LY294002 decreases expression of c-FLIP (cellular FLICE inhibitory protein), an inhibitor of caspase-8 activation. Cycloheximide 0-13 caspase 8 Homo sapiens 115-124 12663669-5 2003 The caspase inhibitor zVADfmk completely blocks caspase activation, DNA degradation, and nuclear fragmentation in both cases but only prevents loss of DeltaPsi and cell death for cytokine plus cycloheximide treatment. Cycloheximide 193-206 caspase 8 Homo sapiens 4-11 12212968-4 2002 The administration of agonistic anti-Fas antibody (CH-11) or cycloheximide alone did not induce apoptosis, whereas the co-administration of CH-11 with cycloheximide induced apoptosis in WI-38 cells, in which caspase-8 and -3, but not -9, were activated, and X chromosome-linked inhibitor of apoptosis (ILP) and FLICE-like inhibitor protein (FLIP(L)), but not bcl-xL and bcl-2, were remarkably down regulated. Cycloheximide 151-164 caspase 8 Homo sapiens 208-224 11278665-7 2001 However, when the cells were sensitized with cycloheximide, which is sufficient to sensitize the cells also to apoptosis by TNF-R1 stimulation, we noticed that adenovirus-mediated expression of constitutively active MKK1 could rescue the cells from apoptosis induced by the respective receptors by preventing caspase-8 activation. Cycloheximide 45-58 caspase 8 Homo sapiens 309-318 11521188-9 2001 These data place potentiating effects of CHX (i) to the activation of caspase 8 at the receptor in LN-229 cells as well as (ii) to a down-stream target at least in LN-18 cells, but probably both cell lines, that may be identical with p21Waf/Cip1. Cycloheximide 41-44 caspase 8 Homo sapiens 70-79 10216102-7 1999 A potent activation of caspase-8 was also induced by cycloheximide, indicating that it was independent of protein synthesis. Cycloheximide 53-66 caspase 8 Homo sapiens 23-32 11187905-7 2000 Furthermore, protein synthesis inhibition by cycloheximide restored sensitivity to CD95-mediated apoptosis and allowed activation of both caspase-8 and caspase-3 in BR97 cells. Cycloheximide 45-58 caspase 8 Homo sapiens 138-147