PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8463287-7 1993 CHO cells transfected with the wild type (CHO/HIR) or a mutant human insulin receptor (CHO/HIRT1336N), in which threonine 1336 was substituted with asparagine, were 32P labeled and then stimulated with insulin or phorbol 12-myristate 13-acetate (PMA). Phosphorus-32 165-168 insulin receptor Homo sapiens 69-85 9115260-3 1997 Phosphopeptide maps of the 32P-labeled CSF1R/IR protein revealed the same pattern of phosphorylation observed in 32P-labeled insulin receptor beta subunits. Phosphorus-32 27-30 insulin receptor Homo sapiens 125-141 9115260-3 1997 Phosphopeptide maps of the 32P-labeled CSF1R/IR protein revealed the same pattern of phosphorylation observed in 32P-labeled insulin receptor beta subunits. Phosphorus-32 113-116 insulin receptor Homo sapiens 125-141 7657666-9 1995 Total cellular tyrosine phosphatase activity was unaltered when assayed with 32P-labeled IR and IRS-1. Phosphorus-32 77-80 insulin receptor Homo sapiens 89-91 8401310-2 1993 The WGA-fraction containing IR and Gir (IR-Gir-fraction) was phosphorylated (95 kDa IR-beta and 66 kDa Gir) by IR-tyrosine kinase using [32P]ATP or photolabeled with [32P]8-azido-GTP (mainly 66 kDa), and was cross-linked with a bifunctional reagent, bis-[sulfosuccinimidyl] suberate (BS3). Phosphorus-32 137-140 insulin receptor Homo sapiens 28-30 8401310-2 1993 The WGA-fraction containing IR and Gir (IR-Gir-fraction) was phosphorylated (95 kDa IR-beta and 66 kDa Gir) by IR-tyrosine kinase using [32P]ATP or photolabeled with [32P]8-azido-GTP (mainly 66 kDa), and was cross-linked with a bifunctional reagent, bis-[sulfosuccinimidyl] suberate (BS3). Phosphorus-32 167-170 insulin receptor Homo sapiens 28-30 1314199-8 1992 When immunoaffinity purified EGF-insulin receptor hybrids and kinase-deficient insulin receptors were used in a cell lysate phosphorylation assay, it was found that addition of EGF produced [32P]-labeling of both receptor species. Phosphorus-32 191-194 insulin receptor Homo sapiens 33-49 2136851-3 1990 Following trypsin digestion, reversed-phase high pressure liquid chromatography (HPLC) analysis of the phosphorylated, affinity-purified insulin receptor preparation reveals phosphopeptide profiles similar to those of trypsin-digested receptors immunoprecipitated from 32P-labeled fibroblasts overexpressing the human insulin receptor. Phosphorus-32 269-272 insulin receptor Homo sapiens 137-153 2583100-5 1989 When immunoaffinity purified EGF-insulin receptor hybrids and kinase-deficient insulin receptors were used in a cell lysate phosphorylation assay, it was found that addition of EGF produced 32P-labeling of both receptor species. Phosphorus-32 190-193 insulin receptor Homo sapiens 33-49 3026445-2 1986 Pretreatment of 32P-labeled insulin receptors with 50 mM DTT followed by gel filtration chromatography in 0.1% SDS demonstrated the dissociation of the alpha 2 beta 2 insulin receptor complex (Mr 400,000) into the monomeric 95,000 beta subunit. Phosphorus-32 16-19 insulin receptor Homo sapiens 28-44 2472754-6 1989 32P-labeled proteins were separated by dodecyl sulfate-gel electrophoresis, digested with trypsin, and then hydrolyzed with 6 N HCl, 2 h, 110 degrees C. Following thin-layer chromatography of the hydrolyzates and autoradiography, phosphotyrosine was detected in insulin receptor substrates, and phosphoserine and phosphothreonine were found in the in vivo-phosphorylated protein. Phosphorus-32 0-3 insulin receptor Homo sapiens 262-278 3234633-3 1988 The digested DNA was subjected to agarose gel electrophoresis, Southern blotted, and hybridised to 32P-labelled human insulin receptor gene cDNA. Phosphorus-32 99-102 insulin receptor Homo sapiens 118-134 2732294-2 1989 Uniformly labeled [32P]antisense RNA probes complementary to insulin receptor mRNA were prepared by an SP6 or T7 RNA polymerase transcription reaction. Phosphorus-32 19-22 insulin receptor Homo sapiens 61-77 2985615-1 1985 When a partially purified insulin receptor preparation immobilized on insulin-agarose is incubated with [gamma-32P]ATP, Mn2+, and Mg2+ ions, the receptor beta subunit becomes 32P-labeled. Phosphorus-32 111-114 insulin receptor Homo sapiens 26-42 3957914-2 1986 The 32P-labeled insulin receptor is isolated by immunoprecipitation with patient-derived insulin receptor antibodies in the presence of phosphatase and protease inhibitors to preserve the native phosphorylation and structural characteristics of the receptor. Phosphorus-32 4-7 insulin receptor Homo sapiens 89-105 3733682-5 1986 The insulin receptor was then isolated from 32P-labeled IM-9 cells that had been exposed to insulin. Phosphorus-32 44-47 insulin receptor Homo sapiens 4-20 2985615-2 1985 The 32P-labeling of the insulin receptor beta subunit is increased by 2-3-fold when src kinase is included in the phosphorylation reaction. Phosphorus-32 4-7 insulin receptor Homo sapiens 24-40 2985615-4 1985 The Mr = 93,000 receptor beta subunit and the Mr = 125,000 32P-labeled bands are absent when an insulin receptor-deficient sample, prepared by the inclusion of excess free insulin to inhibit the adsorption of the receptor to the insulin-agarose, is phosphorylated in the presence of the src kinase. Phosphorus-32 59-62 insulin receptor Homo sapiens 96-112 6757253-2 1982 Addition of insulin to Triton-solubilized extracts of human placental membranes selectively stimulates the incorporation of 32P from [gamma-32P]ATP into an endogenous 95,000-dalton protein, which is identified as a component of the insulin receptor by immunoprecipitation. Phosphorus-32 124-127 insulin receptor Homo sapiens 232-248