PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11889182-5 2002 Bisulfite genomic sequencing revealed a significantly higher mean degree of methylation at each of 12 CpG sites of the H19 promoter in adrenocortical carcinomas than in normal adrenals (P < 0.01 for all sites) or adrenocortical adenomas (P < 0.01, except P < 0.05 for site 12 and P > 0.05 for site 11). hydrogen sulfite 0-9 H19 imprinted maternally expressed transcript Homo sapiens 119-122 23660367-7 2013 As a potential mechanism underlying this disrupted expression, the methylation of Igf2/H19 DMR3 was detected using bisulfite sequencing PCR analysis, which revealed the significant hypomethylation of DMR3 in parthenogenetic fetuses and placentas. hydrogen sulfite 115-124 H19 imprinted maternally expressed transcript Homo sapiens 87-90 19814617-5 2009 In the present study, we investigated a possible method of quantitatively determining H19 hypomethylation in RSS patients using a combined bisulfite restriction analysis (COBRA)-denaturing high-performance liquid chromatography (DHPLC) assay; in this combined assay, polymerase chain reaction products amplified from the H19 differentially methylated region of bisulfite-treated genomic DNA were analyzed using a COBRA assay, which detects methylation-dependent sequence differences in the bisulfite-treated genomic DNA using a restriction enzyme analysis. hydrogen sulfite 139-148 H19 imprinted maternally expressed transcript Homo sapiens 86-89 19814617-5 2009 In the present study, we investigated a possible method of quantitatively determining H19 hypomethylation in RSS patients using a combined bisulfite restriction analysis (COBRA)-denaturing high-performance liquid chromatography (DHPLC) assay; in this combined assay, polymerase chain reaction products amplified from the H19 differentially methylated region of bisulfite-treated genomic DNA were analyzed using a COBRA assay, which detects methylation-dependent sequence differences in the bisulfite-treated genomic DNA using a restriction enzyme analysis. hydrogen sulfite 361-370 H19 imprinted maternally expressed transcript Homo sapiens 86-89 19814617-5 2009 In the present study, we investigated a possible method of quantitatively determining H19 hypomethylation in RSS patients using a combined bisulfite restriction analysis (COBRA)-denaturing high-performance liquid chromatography (DHPLC) assay; in this combined assay, polymerase chain reaction products amplified from the H19 differentially methylated region of bisulfite-treated genomic DNA were analyzed using a COBRA assay, which detects methylation-dependent sequence differences in the bisulfite-treated genomic DNA using a restriction enzyme analysis. hydrogen sulfite 361-370 H19 imprinted maternally expressed transcript Homo sapiens 86-89 18501891-7 2008 Moreover, we analyzed the methylation state of the promoter regions of H19, PHLDA2, and SLC22A18 genes by bisulfite sequencing array and observed a correlation between upregulated expression of H19 and PHLDA2 genes and hypomethylation of their promoter regions, although this was not observed for SLC22A18. hydrogen sulfite 106-115 H19 imprinted maternally expressed transcript Homo sapiens 71-74 18501891-7 2008 Moreover, we analyzed the methylation state of the promoter regions of H19, PHLDA2, and SLC22A18 genes by bisulfite sequencing array and observed a correlation between upregulated expression of H19 and PHLDA2 genes and hypomethylation of their promoter regions, although this was not observed for SLC22A18. hydrogen sulfite 106-115 H19 imprinted maternally expressed transcript Homo sapiens 194-197 29946374-7 2018 Allele-specific changes in the methylation level of H19 ICR in placental tissue (n = 26) and white blood cells (WBC, n = 8) were examined by bisulfite sequencing. hydrogen sulfite 141-150 H19 imprinted maternally expressed transcript Homo sapiens 52-55 26386650-3 2015 Regulation of imprinting of H19 and IGF2 was determined by the DNA methylation status at three CpG sites within the H19 imprinting control region 1 (ICR1) using bisulphite pyrosequencing. hydrogen sulfite 161-171 H19 imprinted maternally expressed transcript Homo sapiens 28-31 26386650-3 2015 Regulation of imprinting of H19 and IGF2 was determined by the DNA methylation status at three CpG sites within the H19 imprinting control region 1 (ICR1) using bisulphite pyrosequencing. hydrogen sulfite 161-171 H19 imprinted maternally expressed transcript Homo sapiens 116-119 23943562-5 2014 Methylation status of H19 DMR in informative samples was analyzed by bisulfite sequencing PCR. hydrogen sulfite 69-78 H19 imprinted maternally expressed transcript Homo sapiens 22-25 28295500-5 2017 The existing evidence along with the absence of relevant data in south of Iran prompted us to study the methylation of H19 imprinted gene in spermatozoa of idiopathic infertile patients (males with abnormalities in sperm parameters) and healthy controls by Combined Bisulfite Restriction Analysis. hydrogen sulfite 266-275 H19 imprinted maternally expressed transcript Homo sapiens 119-122