PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20038516-5 2010 The methylation level of 5" CpG islands in the RASSF1A was evaluated using combined bisulfite restriction analysis and bisulfite sequencing. hydrogen sulfite 84-93 Ras association domain family member 1 Homo sapiens 47-54 20038516-5 2010 The methylation level of 5" CpG islands in the RASSF1A was evaluated using combined bisulfite restriction analysis and bisulfite sequencing. hydrogen sulfite 119-128 Ras association domain family member 1 Homo sapiens 47-54 17322379-5 2007 An inverse relationship between RASSF1A promoter methylation and gene expression was demonstrated by bisulfite sequencing of microdissected placental cells and immunohistochemical staining of placental tissue sections using an anti-RASSF1A antibody. hydrogen sulfite 101-110 Ras association domain family member 1 Homo sapiens 32-39 20642860-4 2010 MATERIALS AND METHODS: Bisulfite pyrosequencing is used to screen 6 putative tumor suppressor genes (HIN-1, RASSF1A, RIL, CDH13, RARbeta2 and E-cadherin) in 38 pairs of primary breast tumors and lymph node metastases. hydrogen sulfite 23-32 Ras association domain family member 1 Homo sapiens 108-115 19024105-9 2008 RASSF1A promoter methylation from cells treated by siRNA-deltaDNMT3B4/2 was detected using MSP and Bisulfite sequencing, and Western blotting was used to detect the protein expression of DNMT3B and ADNMT3B. hydrogen sulfite 99-108 Ras association domain family member 1 Homo sapiens 0-7 12448005-5 2002 Bisulfite-modified DNA was examined for gene promoter hypermethylation in DAP-kinase, E-cadherin, GSTP1, p14, p15, p16, RASSF1A and hMLH1 by methylation-specific-PCR. hydrogen sulfite 0-9 Ras association domain family member 1 Homo sapiens 120-127 14961078-4 2004 Methylation-specific PCR and bisulfite genomic sequencing revealed that the RASSF1A promoter was hypermethylated in all four RASSF1A-nonexpressing cell lines. hydrogen sulfite 29-38 Ras association domain family member 1 Homo sapiens 76-83 12960125-2 2003 In this study, we analyzed the methylation status of the promoter region of RASSF1A using bisulfite sequencing and PCR-RFLP in four liver cancer cell lines (Hep3B, HepG(2), SK-HEP-1, and Huh-7) and a cohort of 43 hepatitis B virus-associated hepatocellular carcinoma (HCC) tissues and their corresponding nontumor tissue specimens. hydrogen sulfite 90-99 Ras association domain family member 1 Homo sapiens 76-83 12912945-10 2003 Real-time methylation-specific PCR system was used for the detection and quantitation of the bisulfite-converted methylated version of the RASSF1A promoter region. hydrogen sulfite 93-102 Ras association domain family member 1 Homo sapiens 139-146 16012945-3 2005 METHODS: The expression and methylation status of 6 RASSF family genes were examined using RT-PCR and bisulfite PCR in CRC cell lines and in primary CRCs and colorectal adenomas. hydrogen sulfite 102-111 Ras association domain family member 1 Homo sapiens 52-57 11559536-9 2001 Bisulfite DNA sequencing analysis revealed that aberrant hypermethylation at the CpG island in the RASSF1A promoter is strongly associated with the loss of RASSF1A expression in cell lines and uncultured primary tumors. hydrogen sulfite 0-9 Ras association domain family member 1 Homo sapiens 99-106 12399230-6 2002 Using bisulphite modification followed by methyl-specific PCR, a high incidence of methylated RASSF1A promoter region was detected in our current series (9/13 cases; 69%). hydrogen sulfite 6-16 Ras association domain family member 1 Homo sapiens 94-101 11585730-4 2001 Bisulfite DNA sequencing analysis revealed that the CpG island in the RASSF1A promoter is hypermethylated in all RASSF1A-nonexpressing cell lines. hydrogen sulfite 0-9 Ras association domain family member 1 Homo sapiens 70-77 11585730-4 2001 Bisulfite DNA sequencing analysis revealed that the CpG island in the RASSF1A promoter is hypermethylated in all RASSF1A-nonexpressing cell lines. hydrogen sulfite 0-9 Ras association domain family member 1 Homo sapiens 113-120 11559536-9 2001 Bisulfite DNA sequencing analysis revealed that aberrant hypermethylation at the CpG island in the RASSF1A promoter is strongly associated with the loss of RASSF1A expression in cell lines and uncultured primary tumors. hydrogen sulfite 0-9 Ras association domain family member 1 Homo sapiens 156-163 30142219-5 2018 METHODS: DNA yield for use in methylation analysis was determined by quantitative methylation specific PCR (qMSP) targeting the ACTB and RASSF1A genes on bisulfite modified DNA. hydrogen sulfite 154-163 Ras association domain family member 1 Homo sapiens 137-144 31242460-3 2019 Bisulfite pyrosequencing and immunohistochemistry were applied to detect RASSF1A gene methylation and PD-L1 protein expression in tumor cells, respectively. hydrogen sulfite 0-9 Ras association domain family member 1 Homo sapiens 73-80 30371994-4 2018 Methylation-specific PCR was performed on the bisulfite-treated DNA against two tumor suppressor genes;RASSF1A and RB. hydrogen sulfite 46-55 Ras association domain family member 1 Homo sapiens 103-110 28871003-5 2017 Promoter methylation of the RASSF1A and O6-methylguanine-DNA-methyltransferase (MGMT) genes was analyzed using bisulfite pyrosequencing of DNA from peripheral blood. hydrogen sulfite 111-120 Ras association domain family member 1 Homo sapiens 28-35 24391879-4 2013 In this study, we have used PAP in combination with bisulfite conversion to develop a new universal methylation-based assay for the detection of fetal methylated RASSF1A sequences in maternal plasma. hydrogen sulfite 52-61 Ras association domain family member 1 Homo sapiens 162-169 25922721-4 2015 DNA samples were extracted from biopsy tissues, serum and plasma and QMSP of APC and RASSF1A was carried out after bisulfite conversion. hydrogen sulfite 115-124 Ras association domain family member 1 Homo sapiens 85-92 24391879-6 2013 Methylation specific primers for PAP were designed for the detection of fetal methylated RASSF1A sequences after bisulfite conversion and validated. hydrogen sulfite 113-122 Ras association domain family member 1 Homo sapiens 89-96 23989600-5 2013 We examined the methylation status of the RASSF1A promoter region by bisulfite pyrosequencing. hydrogen sulfite 69-78 Ras association domain family member 1 Homo sapiens 42-49