PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11245595-6 2001 The transphosphatidylation reaction in the presence of the primary alcohol 1-butanol [leading to stable phosphatidylbutanol (Pbut) formation] was used to measure activity of PLD. Alcohols 67-74 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 174-177 16368959-5 2006 Further, we show that strains of chlamydiae encoding the pzPLD, but not a strain lacking these genes, are inhibited by primary alcohols, potent PLD inhibitors, during growth in HeLa 229 cells. Alcohols 127-135 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 59-62 16113073-8 2006 ANG II-induced PLD activity was inhibited by the primary alcohol n-butanol but not the tertiary alcohol t-butanol. Alcohols 57-64 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 15-18 12217395-4 2002 Primary alcohols specifically interfere with the production of PLD-derived PA and are found to be potent inhibitors of antigen-stimulated exocytosis. Alcohols 8-16 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 63-66 11341962-1 2001 Utilizing the transphosphatidylation reaction catalyzed by phospholipase D (PLD) in the presence of a primary alcohol and the short-chain phospholipid PC8, we have characterized the enzyme from human neutrophils. Alcohols 110-117 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 59-74 11341962-1 2001 Utilizing the transphosphatidylation reaction catalyzed by phospholipase D (PLD) in the presence of a primary alcohol and the short-chain phospholipid PC8, we have characterized the enzyme from human neutrophils. Alcohols 110-117 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 76-79 10873862-4 2000 PLD also catalyses a transphosphatidylation reaction in the presence of phosphatidylcholine and a short-chained primary or secondary alcohol. Alcohols 133-140 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 11154852-0 2000 Inhibition of astroglial cell proliferation by alcohols: interference with the protein kinase C-phospholipase D signaling pathway. Alcohols 47-55 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 96-111 9111933-0 1997 Utilization of alcohols by plant and mammalian phospholipase D. Alcohols 15-23 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 47-62 9111933-3 1997 PLD from peanut utilizes C1 to C8 primary alcohols and gives maximal reaction with butanol. Alcohols 42-50 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 8681428-2 1996 This review summarizes the in vitro evidence showing differential subcellular localization and chromatographic properties of putative PLD isozymes, their phospholipid and alcohol substrate specificities, their modulation by various divalent cations, small G proteins and protein kinase c isozymes, and the role of phosphatidylinositol 4,5-bisphosphate as a cofactor of phospholipase D. Alcohols 171-178 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 134-137 8793299-3 1996 PLD can use primary alcohols in preference to water during the hydrolytic step, generating the corresponding phosphatidylalcohol instead of PA, its normal product. Alcohols 20-28 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 33590314-1 2021 Phosphatidylethanol (PEth) is a group of phospholipids formed in cell membranes following alcohol consumption by action of the enzyme phospholipase D (PLD). Alcohols 90-97 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 134-149 8816991-6 1996 Phospholipase D (PLD) up-regulation was assessed directly by the capacity of PLD to catalyze transphosphatidylation in the presence of alcohol. Alcohols 135-142 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-15 8816991-6 1996 Phospholipase D (PLD) up-regulation was assessed directly by the capacity of PLD to catalyze transphosphatidylation in the presence of alcohol. Alcohols 135-142 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 17-20 8816991-6 1996 Phospholipase D (PLD) up-regulation was assessed directly by the capacity of PLD to catalyze transphosphatidylation in the presence of alcohol. Alcohols 135-142 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 77-80 7772029-1 1995 Phosphatidylalcohol accumulates as a product of a phospholipase D (PLD)-catalysed transphosphatidylation reaction in cells incubated in the presence of a primary alcohol. Alcohols 12-19 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 50-65 7772029-1 1995 Phosphatidylalcohol accumulates as a product of a phospholipase D (PLD)-catalysed transphosphatidylation reaction in cells incubated in the presence of a primary alcohol. Alcohols 12-19 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 67-70 8392931-4 1993 In the presence of an alcohol, PLD converts phosphatidylcholine (PC) into a phosphatidylalcohol (by transphosphatidylation) rather than into PA. Alcohols 22-29 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 31-34 33590314-1 2021 Phosphatidylethanol (PEth) is a group of phospholipids formed in cell membranes following alcohol consumption by action of the enzyme phospholipase D (PLD). Alcohols 90-97 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 151-154 34908404-1 2021 Alcohol consumption leads to formation of phosphatidylethanol (PEth) via the transphosphatidylation activity of phospholipase D (PLD) enzymes. Alcohols 0-7 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 112-127 34908404-1 2021 Alcohol consumption leads to formation of phosphatidylethanol (PEth) via the transphosphatidylation activity of phospholipase D (PLD) enzymes. Alcohols 0-7 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 129-132 3422154-3 1988 A unique feature of PLD is its ability to transfer the phosphatidyl moiety of phospholipids to alcohols (transphosphatidylation). Alcohols 95-103 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 20-23 35276134-6 2022 First, we describe techniques to visualize PA production by phospholipase D (PLD) enzymes, which are major producers of PA, called Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation (IMPACT). Alcohols 179-187 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 60-75 35276134-6 2022 First, we describe techniques to visualize PA production by phospholipase D (PLD) enzymes, which are major producers of PA, called Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation (IMPACT). Alcohols 179-187 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 77-80 35276134-6 2022 First, we describe techniques to visualize PA production by phospholipase D (PLD) enzymes, which are major producers of PA, called Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation (IMPACT). Alcohols 179-187 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 139-154 35276134-7 2022 IMPACT harnesses the ability of endogenous PLD enzymes to accept bioorthogonally tagged alcohols in transphosphatidylation reactions to generate functionalized reporter lipids that are subsequently fluorescently tagged via click chemistry. Alcohols 88-96 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 43-46 31311871-6 2019 Our method capitalizes on the remarkable discovery that bulky, hydrophilic trans-cyclooctene-containing primary alcohols can supplant water as the nucleophile in the PLD active site in a transphosphatidylation reaction of PLD"s lipid substrate, phosphatidylcholine. Alcohols 112-120 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 166-169 32713538-5 2020 Our approach capitalizes on the ability of PLDs to catalyze transphosphatidylation reactions with exogenous alcohols to generate phosphatidyl alcohols, lipids whose location and abundance report on the extent of PLD-mediated PA synthesis. Alcohols 108-116 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 43-46 32713538-6 2020 Our key innovation is to employ functionalized, "clickable," alcohols as PLD substrates, which enables subsequent tagging of the resultant phosphatidyl alcohols with fluorophores or other functional probes for detection via highly selective click chemistry reactions. Alcohols 61-69 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 73-76 32713538-7 2020 In this chapter, we describe this method, termed IMPACT (Imaging PLD Activity with Clickable Alcohols via Transphosphatidylation), which can be coupled to downstream analysis by fluorescence microscopy, flow cytometry, HPLC, or mass spectrometry. Alcohols 93-101 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 65-68 33843991-0 2021 Structures of an engineered phospholipase D with specificity for secondary alcohol transphosphatidylation: Insights into plasticity of substrate binding and activation. Alcohols 75-82 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 28-43 31311871-6 2019 Our method capitalizes on the remarkable discovery that bulky, hydrophilic trans-cyclooctene-containing primary alcohols can supplant water as the nucleophile in the PLD active site in a transphosphatidylation reaction of PLD"s lipid substrate, phosphatidylcholine. Alcohols 112-120 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 222-225 23681537-4 2013 The protocol takes advantage of a unique property of PLD, i.e., its ability to substitute a primary alcohol, such as 1-butanol, for water in the hydrolytic reaction. Alcohols 100-107 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 53-56 29104923-7 2017 Our method, termed IMPACT (Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation), reveals pools of basal and stimulated PLD activities in expected and unexpected locations. Alcohols 75-83 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 35-50 29104923-7 2017 Our method, termed IMPACT (Imaging Phospholipase D Activity with Clickable Alcohols via Transphosphatidylation), reveals pools of basal and stimulated PLD activities in expected and unexpected locations. Alcohols 75-83 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 151-154 25275439-2 2014 Like other primary alcohols, 1-butanol is a substrate for PLD and thereby disrupts formation of the intracellular signaling lipid phosphatidic acid. Alcohols 19-27 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 58-61 23895284-0 2013 Activation of AMPK/TSC2/PLD by alcohol regulates mTORC1 and mTORC2 assembly in C2C12 myocytes. Alcohols 31-38 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 24-27 23500715-5 2013 Adding of primary alcohol (1-butanol) to the incubation medium led to phosphatidylbutanol accumulation as a result of phospholipase D (PLD) action in wild-type and NADPH-oxidase RbohD deficient plants. Alcohols 18-25 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 118-133 23500715-5 2013 Adding of primary alcohol (1-butanol) to the incubation medium led to phosphatidylbutanol accumulation as a result of phospholipase D (PLD) action in wild-type and NADPH-oxidase RbohD deficient plants. Alcohols 18-25 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 135-138 28365224-2 2017 The method utilizes the transphosphatidylation ability of bacterial PLD on alcohol groups and the hydrolysis activity of overexpressed PLD on phospholipids in cancer cells. Alcohols 75-82 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 68-71 24618697-6 2014 Treatment of cells with the primary alcohol 1-butanol inhibits the hydrolysis of phosphatidylcoline by PLD thereby suppressing phosphatidic acid (PA) production. Alcohols 36-43 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 103-106 22579635-5 2012 The production of PA is abrogated by primary alcohols that have been widely used for the analysis of PLD-mediated processes. Alcohols 45-53 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 101-104 20372065-0 2010 Reduced mortality and moderate alcohol consumption: the phospholipase D-mTOR connection. Alcohols 31-38 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 56-71 20601268-8 2011 (3) Secondary and tertiary alcohols and hydroquinone are difficult to transphosphatidylate by PLD. Alcohols 27-35 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 94-97 20932007-1 2010 The design of stable redox active liposomes where the organometallic electroactive pendent was covalently bound to the phospholipid headgroup through a phospholipase D (PLD)-catalyzed transphosphatidylation reaction between a choline-bearing phospholipid and a primary alcohol containing a ferrocene derivative is reported. Alcohols 269-276 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 152-167 20932007-1 2010 The design of stable redox active liposomes where the organometallic electroactive pendent was covalently bound to the phospholipid headgroup through a phospholipase D (PLD)-catalyzed transphosphatidylation reaction between a choline-bearing phospholipid and a primary alcohol containing a ferrocene derivative is reported. Alcohols 269-276 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 169-172 19845506-9 2009 Primary alcohols, used previously as a means to reduce PLD-derived phosphatidic acid, were potent inhibitors of membrane blebbing, but a more selective inhibitor of PLD, FIPI (5-fluoro-2-indolyl des-chlorohalopemide), was without effect. Alcohols 8-16 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 55-58 19845506-9 2009 Primary alcohols, used previously as a means to reduce PLD-derived phosphatidic acid, were potent inhibitors of membrane blebbing, but a more selective inhibitor of PLD, FIPI (5-fluoro-2-indolyl des-chlorohalopemide), was without effect. Alcohols 8-16 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 165-168 19064628-2 2009 PLD production of PA is inhibited by the primary alcohol 1-butanol, which has thus been widely employed to identify PLD/PA-driven processes. Alcohols 49-56 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 19064628-2 2009 PLD production of PA is inhibited by the primary alcohol 1-butanol, which has thus been widely employed to identify PLD/PA-driven processes. Alcohols 49-56 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 116-119 17151288-8 2007 Perturbation of this signaling pathway via inhibition of lipid phosphate phosphatase-1 (LPP-1) by propranolol or inhibition of the phosphatidylcholine-derived phosphatidic acid (PA) formation by PLD with a primary alcohol significantly attenuated platelet activation by PAR1-AP. Alcohols 214-221 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 195-198