PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24887377-4 2014 In the present study, we incorporated an alkyne-bearing NAA into an enzyme, murine dihydrofolate reductase (mDHFR), in high cell density cultivation of Escherichia coli, and performed CuAAC conjugation with fluorescent azide dyes to evaluate enzyme compatibility of various CuAAC conditions comprising combination of commercially available Cu(I)-chelating ligands and reductants. Azides 219-224 dihydrofolate reductase Mus musculus 108-113 6189586-4 1983 However, glucose or azide increased the fraction of dihydrofolate reductase associated with methotrexate and abolished the effect of tetrahydrofolates on this intracellular component. Azides 20-25 dihydrofolate reductase Mus musculus 52-75