PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20709787-3 2010 We have previously described a genetically engineered C. albicans tet-NRG1 strain in which NRG1 expression levels can be manipulated by the presence or absence of doxycycline (DOX). tetramethylenedisulfotetramine 66-69 transcriptional regulator NRG1 Saccharomyces cerevisiae S288C 70-74 20709787-3 2010 We have previously described a genetically engineered C. albicans tet-NRG1 strain in which NRG1 expression levels can be manipulated by the presence or absence of doxycycline (DOX). tetramethylenedisulfotetramine 66-69 transcriptional regulator NRG1 Saccharomyces cerevisiae S288C 91-95 20709787-5 2010 In an in vitro model of biofilm formation, the C. albicans tet-NRG1 strain was able to form mature biofilms only when DOX was present in the medium, but not in the absence of DOX, when high levels of NRG1 expression blocked the yeast-to-hypha transition. tetramethylenedisulfotetramine 59-62 transcriptional regulator NRG1 Saccharomyces cerevisiae S288C 63-67 20709787-5 2010 In an in vitro model of biofilm formation, the C. albicans tet-NRG1 strain was able to form mature biofilms only when DOX was present in the medium, but not in the absence of DOX, when high levels of NRG1 expression blocked the yeast-to-hypha transition. tetramethylenedisulfotetramine 59-62 transcriptional regulator NRG1 Saccharomyces cerevisiae S288C 200-204 20709787-6 2010 However, in a biofilm cell retention assay in which biofilms were developed with mixtures of C. albicans tet-NRG1 and SC5314 strains, tet-NRG1 yeast cells were still incorporated into the mixed biofilms, in which an intricate network of hyphae of the wild-type strain provided for biofilm structural integrity and adhesive interactions. tetramethylenedisulfotetramine 105-108 transcriptional regulator NRG1 Saccharomyces cerevisiae S288C 109-113