PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8240373-0 1993 Detection of K-ras codon 12 mutation by polymerase chain reaction and differential dot-blot hybridization in sodium saccharin-treated human RSA cell. Saccharin 109-125 KRAS proto-oncogene, GTPase Homo sapiens 13-18 7671231-2 1995 Sodium saccharin-induced mutagenicity is detectable in human RSa cells by estimation of cloning efficiency of ouabain-resistant mutant cells and determination of K-ras codon 12 mutation in genomic DNA, analyzed by PCR and differential dot-blot hybridization. Saccharin 0-16 KRAS proto-oncogene, GTPase Homo sapiens 162-167 8240373-1 1993 Using polymerase chain reaction and differential dot-blot hybridization, point mutations of K-ras codon 12 were investigated in hyper-mutable human RSa cells treated with sodium saccharin. Saccharin 171-187 KRAS proto-oncogene, GTPase Homo sapiens 92-97 8240373-3 1993 Results imply that sodium saccharin is a mutagen which induces genetic mutation and, furthermore, that the assay system applied here will be beneficial in identifying mutagen-induced K-ras oncogene mutations in human cells in vitro. Saccharin 19-35 KRAS proto-oncogene, GTPase Homo sapiens 183-188