PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1835828-0 1991 Immunoelectron microscopy of the calcium-binding protein synexin in isolated adrenal chromaffin granules and chromaffin cells. chromaffin 85-95 annexin A7 Homo sapiens 57-64 1911746-7 1991 Ca(2+)-dependent chromaffin granule membrane aggregation, induced by p32 or synexin, was associated with intermembrane annexin polymerization at Ca2+ levels less than pCa 4, but not at higher Ca2+ concentrations, suggesting that annexin self-association may be necessary for membrane contact at low Ca2+ levels but not at higher Ca2+ levels where the protein may bind two membranes as a monomer. chromaffin 17-27 annexin A7 Homo sapiens 76-83 1835828-0 1991 Immunoelectron microscopy of the calcium-binding protein synexin in isolated adrenal chromaffin granules and chromaffin cells. chromaffin 109-119 annexin A7 Homo sapiens 57-64 6224488-0 1983 Calcium dependence of the binding of synexin to isolated chromaffin granules. chromaffin 57-67 annexin A7 Homo sapiens 37-44 2954851-1 1987 The mechanism by which synexin mediates calcium-dependent aggregation of medullary cell chromaffin granules and fusion of granule ghosts involves specific interactions with the lipid component of the membrane. chromaffin 88-98 annexin A7 Homo sapiens 23-30 3155695-0 1985 Synexin binds in a calcium-dependent fashion to oriented chromaffin cell plasma membranes. chromaffin 57-67 annexin A7 Homo sapiens 0-7 3155695-2 1985 Synexin binding was also detected on beads coated with chromaffin granule membranes, but not to beads coated with erythrocyte membranes or to uncoated beads. chromaffin 55-65 annexin A7 Homo sapiens 0-7 2443172-0 1987 Effect of synexin on aggregation and fusion of chromaffin granule ghosts at pH 6. chromaffin 47-57 annexin A7 Homo sapiens 10-17 2443172-1 1987 Fusion of chromaffin granule ghosts was induced by synexin at pH 6, 37 degrees C, in the presence of 10(-7) M Ca2+. chromaffin 10-20 annexin A7 Homo sapiens 51-58 6224488-1 1983 The calcium dependence of the binding of synexin to isolated chromaffin granules has been investigated. chromaffin 61-71 annexin A7 Homo sapiens 41-48 6224488-5 1983 The data therefore suggest that in the chromaffin cell stimulated to release catecholamines and proteins by exocytosis synexin first binds to membranes and then associates with itself to draw membranes together in preparation for fusion. chromaffin 39-49 annexin A7 Homo sapiens 119-126 6452452-2 1981 Synexin, a soluble adrenal medullary and liver protein which causes calcium-dependent aggregation of isolated chromaffin granules, was isolated and purified according to published procedures. chromaffin 110-120 annexin A7 Homo sapiens 0-7 6220716-1 1983 Synexin, an approximately 47,000 Mr soluble protein isolated from adrenal medulla or liver, shows Ca2+-specific enhancement of the aggregation of chromaffin granule or other negatively charged biological or artificial membranes. chromaffin 146-156 annexin A7 Homo sapiens 0-7 6218998-0 1982 Synhibin: a new calcium-dependent membrane-binding protein that inhibits synexin-induced chromaffin granule aggregation and fusion. chromaffin 89-99 annexin A7 Homo sapiens 73-80 6211522-1 1982 Synexin, a protein from the cytosol of the adrenal medulla, selectively increases the ability of Ca2+ to aggregate chromaffin granules and other membrane-bound particles. chromaffin 115-125 annexin A7 Homo sapiens 0-7 6211522-2 1982 The ability of synexin to self-aggregate in the presence of Ca2+ can be employed in the purification of the protein by monitoring purification with parallel assays that utilize the aggregation of both chromaffin granule membranes and phosphatidylserine liposomes. chromaffin 201-211 annexin A7 Homo sapiens 15-22 6223635-3 1983 These data thus indicate that synexin may be a target of phenothiazine drugs when they block secretion from chromaffin cells. chromaffin 108-118 annexin A7 Homo sapiens 30-37 6457840-0 1981 cis-Unsaturated fatty acids induce the fusion of chromaffin granules aggregated by synexin. chromaffin 49-59 annexin A7 Homo sapiens 83-90 6457840-1 1981 When isolated chromaffin granules were aggregated by synexin (a Ca2+-binding protein present in chromaffin and other secretory tissues) and then exposed to cis-unsaturated fatty acids at 37 degrees C, they fused together to form large vesicles. chromaffin 14-24 annexin A7 Homo sapiens 53-60 6457840-1 1981 When isolated chromaffin granules were aggregated by synexin (a Ca2+-binding protein present in chromaffin and other secretory tissues) and then exposed to cis-unsaturated fatty acids at 37 degrees C, they fused together to form large vesicles. chromaffin 96-106 annexin A7 Homo sapiens 53-60 632306-0 1978 Identification and purification of an adrenal medullary protein (synexin) that causes calcium-dependent aggregation of isolated chromaffin granules. chromaffin 128-138 annexin A7 Homo sapiens 65-72 6449199-0 1980 Specificity of synexin-induced chromaffin granule aggregation. chromaffin 31-41 annexin A7 Homo sapiens 15-22 762079-5 1979 253, 2858-2866) that synexin, an adrenal medullary protein that causes Ca2+-dependent aggregation of isolated chromaffin granules, might be the intracellular receptor for Ca2+ in the process of exocytosis. chromaffin 110-120 annexin A7 Homo sapiens 21-28 762079-9 1979 The curve has a Hill coefficient of 2.3 and is half-maximal at 200 micron Ca2+, which correlates exactly with the Ca2+ titration curve for the aggregation of chromaffin granules by synexin. chromaffin 158-168 annexin A7 Homo sapiens 181-188 762079-11 1979 We suggest that synexin exists as a soluble monomer in the cytoplasm of the resting chromaffin cell and that when the cell is stimulated to secrete, the increase in the intracellular concentrations of Ca2+ causes synexin to form rods, similar to those formed in vitro, which bind to chromaffin granule and plasma membranes, joining them together to form the "pentalaminar" fusion complexes characteristic of the first step in exocytosis. chromaffin 84-94 annexin A7 Homo sapiens 16-23 762079-11 1979 We suggest that synexin exists as a soluble monomer in the cytoplasm of the resting chromaffin cell and that when the cell is stimulated to secrete, the increase in the intracellular concentrations of Ca2+ causes synexin to form rods, similar to those formed in vitro, which bind to chromaffin granule and plasma membranes, joining them together to form the "pentalaminar" fusion complexes characteristic of the first step in exocytosis. chromaffin 283-293 annexin A7 Homo sapiens 16-23 11994295-5 2002 In vivo, the ratio of bound GDP/GTP and phosphorylation of annexin 7 change in direct proportion to the extent of catecholamine release from chromaffin cells in response to stimulation by carbachol, or to inhibition by various protein kinase C inhibitors. chromaffin 141-151 annexin A7 Homo sapiens 59-68 11994295-6 2002 These results thus lead us to hypothesize that annexin 7 may serve as a common site of action for calcium, guanine nucleotide, and protein kinase C in the exocytotic membrane fusion process in chromaffin cells. chromaffin 193-203 annexin A7 Homo sapiens 47-56 9268363-8 1997 Additionally, synexin forms a complex with sorcin and recruits this protein to chromaffin granule membranes in a Ca2+-dependent manner. chromaffin 79-89 annexin A7 Homo sapiens 14-21 9268363-9 1997 Sorcin is able to inhibit synexin-mediated chromaffin granule aggregation in a manner saturable with increasing sorcin concentrations, but does not influence the Ca2+ sensitivity of synexin-mediated granule aggregation. chromaffin 43-53 annexin A7 Homo sapiens 26-33 12438137-0 2002 Annexin 7: a non-SNARE proteolytic substrate for botulinum toxin type C in secreting chromaffin cells. chromaffin 85-95 annexin A7 Homo sapiens 0-9 11278415-1 2001 Annexin 7, a Ca(2+)/GTP-activated membrane fusion protein, is preferentially phosphorylated in intact chromaffin cells, and the levels of annexin 7 phosphorylation increase quantitatively in proportion to the extent of catecholamine secretion. chromaffin 102-112 annexin A7 Homo sapiens 0-9 11278415-7 2001 These results thus lead us to hypothesize that annexin 7 may be a positive mediator for protein kinase C action in the exocytotic membrane fusion reaction in chromaffin cells. chromaffin 158-168 annexin A7 Homo sapiens 47-56 8855260-3 1996 The mechanism of fusion activation depends on the unique ability of synexin to bind and hydrolyze GTP in a calcium-dependent manner, both in vitro and in vivo in streptolysin O-permeabilized chromaffin cells. chromaffin 191-201 annexin A7 Homo sapiens 68-75 8855260-5 1996 Previous immunolocalization studies place synexin at exocytotic sites in chromaffin cells, and we conclude that synexin is an atypical G protein that may be responsible for both detecting and mediating the Ca2+/GTP signal for exocytotic membrane fusion. chromaffin 73-83 annexin A7 Homo sapiens 112-119