PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 29467863-15 2018 CastPCR technology is a robust method with high sensitivity for the molecular detection of EGFR and BRAF mutations in clinical formalin-fixed paraffin-embedded samples. Paraffin 142-150 epidermal growth factor receptor Homo sapiens 91-95 28750448-6 2017 ErbB mRNA expression levels were determined after extraction from formalin-fixed, paraffin-embedded tissue. Paraffin 82-90 epidermal growth factor receptor Homo sapiens 0-4 29262544-3 2017 We evaluated the Idylla EGFR Mutation Assay (Biocartis) for the detection of EGFR mutations in archived formalin-fixed paraffin-embedded (FFPE) tumor samples from a series of 55 patients with lung adenocarcinoma and compared these results with those obtained by a pyrosequencing ISO-15189 accredited laboratory method. Paraffin 120-128 epidermal growth factor receptor Homo sapiens 78-82 28720682-0 2017 Reducing Artifactual EGFR T790M Mutations in DNA from Formalin-Fixed Paraffin-Embedded Tissue by Use of Thymine-DNA Glycosylase. Paraffin 69-77 epidermal growth factor receptor Homo sapiens 21-25 28589341-8 2017 However, droplet digital polymerase chain reaction is a promising and reliable assay that can be used as a diagnostic tool to genotype formalin-fixed paraffin-embedded cancer samples for EGFR or another clinically relevant somatic mutation. Paraffin 150-158 epidermal growth factor receptor Homo sapiens 187-191 28625519-5 2017 All 33 EGFR-activating mutations detected in formalin-fixed paraffin-embedded (FFPE) tissue samples by the conventional method were also detected by this multiplex assay. Paraffin 60-68 epidermal growth factor receptor Homo sapiens 7-11 28468035-4 2017 Results: There were 173 cases with EGFR gene mutation in 582 cases of paraffin-embedded tissue in patients with NSCLC, and the mutation rate was 29.7%(173/582). Paraffin 70-78 epidermal growth factor receptor Homo sapiens 35-39 28026806-3 2016 The aim of this study was to design a real-time PCR-based diagnostic kit for fast and cheap of EGFR mutations testing in paraffin blocks and plasma, and kit validation using samples from patients with NSCLC, and also comparative estimation of diagnostic features of real-time PCR with wild type blocking and digital PCR for mutation testing in blood plasma. Paraffin 121-129 epidermal growth factor receptor Homo sapiens 95-99 27737606-5 2016 Expert commentary: The therascreen kit is a highly sensitive real-time polymerase chain reaction test that provides standardised testing and automated interpretation of EGFR mutation status in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Paraffin 210-218 epidermal growth factor receptor Homo sapiens 170-174 28026806-6 2016 Kit for EGFR mutations was validated using 411 samples of paraffin blocks. Paraffin 58-66 epidermal growth factor receptor Homo sapiens 8-12 27215400-3 2016 METHODS: EGFR mutation status was assessed for lung adenocarcinoma samples (formalin fixed- paraffin embedded samples) using qPCR, SNaPshot and NGS (Ion Torrent ) techniques. Paraffin 92-100 epidermal growth factor receptor Homo sapiens 9-13 27565930-2 2016 MATERIALS AND METHODS: The peptide nucleic acid-locked nucleic acid polymerase chain reaction clamp method was used to detect mutations in exons 18, 19, 20, and 21 of the EGFR gene in DNA samples extracted from paraffin-embedded tissue sections. Paraffin 211-219 epidermal growth factor receptor Homo sapiens 171-175 27053957-3 2016 MATERIALS AND METHODS: Expression of EGFR, c-Met, and HER2 protein was detected by immunohistochemistry (IHC) in 180 paraffin-embedded tissue samples from stage IIB-IIIC ESCC patients. Paraffin 117-125 epidermal growth factor receptor Homo sapiens 37-41 26961138-5 2016 Relying on extracted DNAs from the paraffin-embedded tumor samples, EGFR mutations were detected by direct sequencing. Paraffin 35-43 epidermal growth factor receptor Homo sapiens 68-72 27461620-3 2016 This study aims to report on the prevalence of EGFR mutations in NSCLC and non-squamous lung cancer patients at diagnosis, using genomic deoxyribonucleic acid (DNA) obtained from paraffin-embedded tissue samples. Paraffin 179-187 epidermal growth factor receptor Homo sapiens 47-51 26891729-1 2016 therascreen( ) EGFR RGQ PCR Kit is a real-time polymerase chain reaction test kit for the qualitative detection of exon 19 deletions and L858R mutation of the human EGFR gene in formalin-fixed paraffin-embedded non-small cell lung cancer (NSCLC) tissue. Paraffin 193-201 epidermal growth factor receptor Homo sapiens 15-19 26891729-1 2016 therascreen( ) EGFR RGQ PCR Kit is a real-time polymerase chain reaction test kit for the qualitative detection of exon 19 deletions and L858R mutation of the human EGFR gene in formalin-fixed paraffin-embedded non-small cell lung cancer (NSCLC) tissue. Paraffin 193-201 epidermal growth factor receptor Homo sapiens 165-169 26240388-6 2015 To demonstrate the clinical applicability of our assay, we show specific detection of a point mutation in the EGFR gene in fresh frozen and formalin-fixed, paraffin-embedded (FFPE) lung cancer samples and confirm the detected mutation by in situ sequencing. Paraffin 156-164 epidermal growth factor receptor Homo sapiens 110-114 27516024-2 2016 The aim of this study was to identify patients with EGFR mutations using allele-specific polymerase chain reaction (PCR), from formalin-fixed paraffin-embedded (FFPE) tissue and fresh tissue (FT). Paraffin 142-150 epidermal growth factor receptor Homo sapiens 52-56 25525358-4 2014 MATERIALS AND METHODS: EGFR gene mutations were detected by QDs-IHC, IHC, and ADx-ARMS in 65 cases of NSCLC composed of 55 formalin-fixed, paraffin-embedded specimens and ten pleural effusion cell blocks, including 13 squamous cell carcinomas, two adenosquamous carcinomas, and 50 adenocarcinomas. Paraffin 139-147 epidermal growth factor receptor Homo sapiens 23-27 25651992-7 2015 After pathological diagnoses of NSCLC, EGFR mutation status in formalin-fixed, paraffin- embedded samples was confirmed by the PCR-invader method, and the concordance rates between the PCR methods were compared. Paraffin 79-87 epidermal growth factor receptor Homo sapiens 39-43 26081767-1 2015 BACKGROUND: The purpose of this study was to develop a cost-effective approach for the determination of EGFR and KRAS mutations in formalin-fixed paraffin-embedded (FFPE) non-small cell lung cancer (NSCLC) and colorectal cancer (CRC) samples from Chinese patients based on a sensitive pyrosequencing (PS) technique. Paraffin 146-154 epidermal growth factor receptor Homo sapiens 104-108 25679210-4 2015 METHODS: In the present study, immunohistochemical staining of EGFR and CXCR4 was performed on paraffin-embedded specimens from 131 patients with surgically resected PDAC. Paraffin 95-103 epidermal growth factor receptor Homo sapiens 63-67 25494138-4 2014 Aim: In this research work, the over expression of EGFR in formalin fixed paraffin-embedded (FFPE) colorectal cancer tumor tissue of patients was studied. Paraffin 74-82 epidermal growth factor receptor Homo sapiens 51-55 25263855-2 2014 EGFR mutations are commonly tested by Sanger sequencing or allele specific polymerase chain reaction (ASPCR) on formalin-fixed paraffin-embedded (FFPE) samples including cell blocks (CB) that may fail due to absence of tumor cells. Paraffin 127-135 epidermal growth factor receptor Homo sapiens 0-4 24788590-4 2014 EGFR/p53 mutational analysis was performed on DNA extracted from paraffin-embedded tumors. Paraffin 65-73 epidermal growth factor receptor Homo sapiens 0-4 25674250-4 2014 In the 22 matched samples, 13 cases of EGFR mutations were identified in paraffin-embedded biopsy tissue samples, 12 cases in the cell-free supernatant and 9 cases in the cell pellets of pleural effusion. Paraffin 73-81 epidermal growth factor receptor Homo sapiens 39-43 24528049-3 2014 MATERIALS AND METHODS: In this prospective study, EGFR mutations in exons 18, 19, 20 and 21 in formalin-fixed paraffin-embedded biopsy specimens of consecutive NSCLC patients were asessed by real-time polymerase chain reaction. Paraffin 110-118 epidermal growth factor receptor Homo sapiens 50-54 24377577-0 2014 High resolution melting analysis for epidermal growth factor receptor mutations in formalin-fixed paraffin-embedded tissue and plasma free DNA from non-small cell lung cancer patients. Paraffin 98-106 epidermal growth factor receptor Homo sapiens 37-69 23621958-0 2013 Analytic performance studies and clinical reproducibility of a real-time PCR assay for the detection of epidermal growth factor receptor gene mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancer. Paraffin 170-178 epidermal growth factor receptor Homo sapiens 104-136 23892415-4 2013 We had evaluated the frequency of EGFR mutations with three highly sensitive PCR techniques in formalin-fixed, paraffin-embedded samples of 143 NSCLC patients with central nervous system (CNS) metastases. Paraffin 111-119 epidermal growth factor receptor Homo sapiens 34-38 24890176-0 2014 A simple and efficient method of DNA extraction from formalin-fixed and paraffin-embedded tissues for screening EGFR gene mutation by gene sequencing. Paraffin 72-80 epidermal growth factor receptor Homo sapiens 112-116 23386666-0 2013 Comparison of molecular testing methods for the detection of EGFR mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancer. Paraffin 94-102 epidermal growth factor receptor Homo sapiens 61-65 23386666-1 2013 AIM: To conduct a methods correlation study of three different assays for the detection of mutations at EGFR gene in human formalin-fixed paraffin-embedded tumour (FFPET) specimens of non-small cell lung carcinomas (NSCLC). Paraffin 138-146 epidermal growth factor receptor Homo sapiens 104-108 23769435-2 2013 METHODS: With Q-scoring assessment and ROC curve analysis,immunohistochemistry was used to screen a panel of 309 paraffin-embedded non-small cell lung cancer(NSCLC) tumor samples, of which EGFR gene mutational status was previously documented by PCR-DNA sequencing. Paraffin 113-121 epidermal growth factor receptor Homo sapiens 189-193 23590575-0 2013 Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients. Paraffin 74-82 epidermal growth factor receptor Homo sapiens 13-45 23590575-3 2013 The aim of this study was to detect EGFR mutations in formalin fixed paraffin embedded (FFPE) samples using both Scorpion ARMS and high resolution melt (HRM) assay, and to compare the sensitivity of these methods. Paraffin 69-77 epidermal growth factor receptor Homo sapiens 36-40 23769436-5 2013 CONCLUSION: With high sensitivity and good specificity, immunohistochemistry using EGFR mutation-specific monoclonal antibodies is an adequate, easy and cost-effective prescreening method to detect EGFR mutations using paraffin-embedded tissue specimens of lung adenocarcinomas. Paraffin 219-227 epidermal growth factor receptor Homo sapiens 83-87 23769436-5 2013 CONCLUSION: With high sensitivity and good specificity, immunohistochemistry using EGFR mutation-specific monoclonal antibodies is an adequate, easy and cost-effective prescreening method to detect EGFR mutations using paraffin-embedded tissue specimens of lung adenocarcinomas. Paraffin 219-227 epidermal growth factor receptor Homo sapiens 198-202 23886169-2 2013 MATERIALS AND METHODS: EGFR gene exons 18-21 in 48 specimens of paraffin-embedded tumor tissue from NSCLC patients were amplified by PCR, followed by direct sequencing and analysis of links to clinicopathological features and prognosis. Paraffin 64-72 epidermal growth factor receptor Homo sapiens 23-27 23483646-1 2013 BACKGROUND: The aims of this study were to evaluate the abilities of direct sequencing (DS), peptide nucleic acid (PNA) clamping, and pyrosequencing methods to detect epidermal growth factor receptor (EGFR) mutations in formalin-fixed paraffin-embedded (FFPE) non-small cell lung carcinoma (NSCLC) samples and to correlate EGFR mutational status as determined by each method with the clinical response to EGFR tyrosine kinase inhibitors (TKIs). Paraffin 235-243 epidermal growth factor receptor Homo sapiens 167-199 23483646-1 2013 BACKGROUND: The aims of this study were to evaluate the abilities of direct sequencing (DS), peptide nucleic acid (PNA) clamping, and pyrosequencing methods to detect epidermal growth factor receptor (EGFR) mutations in formalin-fixed paraffin-embedded (FFPE) non-small cell lung carcinoma (NSCLC) samples and to correlate EGFR mutational status as determined by each method with the clinical response to EGFR tyrosine kinase inhibitors (TKIs). Paraffin 235-243 epidermal growth factor receptor Homo sapiens 201-205 23055340-5 2012 METHODS: EGFR protein expression was studied in 98 paraffin embedded tissue samples, organized in a tissue microarray. Paraffin 51-59 epidermal growth factor receptor Homo sapiens 9-13 23691506-8 2013 EGFR mutation analysis using formalin-fixed paraffin-embedded lung cancer tissues demonstrated a mutant peak with Method A, whereas the mutant peak was undetectable with Methods B or C. Method A yielded the best DNA quantity and quality with outstanding efficiency, particularly when paucicellular specimens were used. Paraffin 44-52 epidermal growth factor receptor Homo sapiens 0-4 23648296-9 2013 CONCLUSIONS: The success rate of ADx-EGFR real-time PCR for formalin-fixed and paraffin-embedded tissues samples is significantly higher than that of Sanger sequencing (P < 0.01). Paraffin 79-87 epidermal growth factor receptor Homo sapiens 37-41 23803047-2 2013 METHODS: The Scorpions amplification refractory mutation system (Scorpions ARMS) was used to measure mutations in exons 18, 19, 20 and 21 of the EGFR gene in paraffin-embedded tumor tissue from NSCLC cases, and statistical analysis was performed to investigate links with clinicopathological features in different histological types of NSCLC. Paraffin 158-166 epidermal growth factor receptor Homo sapiens 145-149 21681971-10 2013 The presence of prominent INCI (P = 0.036), papillary fragments (P = 0.041), and histologic features of BAC on paraffin block (P = 0.039) correlated with the presence of EGFR mutations. Paraffin 111-119 epidermal growth factor receptor Homo sapiens 170-174 23841222-0 2013 Detection of the epidermal growth factor receptor, amphiregulin and epiregulin in formalin-fixed paraffin-embedded human placenta tissue by matrix-assisted laser desorption/ionization mass spectrometry imaging. Paraffin 97-105 epidermal growth factor receptor Homo sapiens 17-49 23181982-5 2012 We retrospectively analyzed EGFR gene copy number by fluorescence in situ hybridization (FISH) in paraffin-embedded tissue from 215 patients recruited through a prospective colorectal cancer screening procedure and undergoing surgical colectomy. Paraffin 98-106 epidermal growth factor receptor Homo sapiens 28-32 22923193-5 2012 EGFR mutations of 126 Formalin-Fixed Paraffin-Embedded (FFPE), 47 fresh frozen tissues and from 47 matched pre-operation serum specimens of NSCLC patients were screened by the HRM assays. Paraffin 37-45 epidermal growth factor receptor Homo sapiens 0-4 23153332-3 2012 METHODS: EGFR status was analyzed by immunohistochemistry (IHC) in paraffin-embedded samples from 357 patients who received chemotherapy in 4 first-line trials. Paraffin 67-75 epidermal growth factor receptor Homo sapiens 9-13 20652725-6 2011 EGFR immunohistochemistry staining was performed on paraffin-embedded sections with a mouse monoclonal antibody (clone 2-18C9; Dako). Paraffin 52-60 epidermal growth factor receptor Homo sapiens 0-4 22406363-10 2012 Mutation analysis of EGFR exons 19 and 21 is feasible in microdissected paraffin sections from archival tissues. Paraffin 72-80 epidermal growth factor receptor Homo sapiens 21-25 21391216-0 2012 Profiling signalling pathways in formalin-fixed and paraffin-embedded breast cancer tissues reveals cross-talk between EGFR, HER2, HER3 and uPAR. Paraffin 52-60 epidermal growth factor receptor Homo sapiens 119-123 22321542-2 2011 METHODS: Optimized oligonucleotide probe method was applied to detect EGFR mutations involving exons 18 - 21 using formalin fixed paraffin embedded tissue specimens of 309 NSCLC patients. Paraffin 130-138 epidermal growth factor receptor Homo sapiens 70-74 22321547-2 2011 METHODS: The EGFR gene exons 19 and 21 of paraffin-embedded tumor tissue were amplified by PCR, followed by direct sequencing in 282 surgically-removed specimens of NSCLC. Paraffin 42-50 epidermal growth factor receptor Homo sapiens 13-17 21908901-6 2011 Finally, we applied our assay to assess EGFR/HER2 dimerization in paraffin embedded cell pellets. Paraffin 66-74 epidermal growth factor receptor Homo sapiens 40-44 20331873-4 2010 EGFR gene amplification was studied by fluorescence in situ hybridization using paraffin-embedded tissues. Paraffin 80-88 epidermal growth factor receptor Homo sapiens 0-4 20405295-2 2011 METHODS: p16 and EGFR expression was investigated by immunohistochemistry from paraffin-embedded tissue in 39 patients with adenocarcinoma of the uterine cervix. Paraffin 79-87 epidermal growth factor receptor Homo sapiens 17-21 21118047-5 2011 RESULTS: A total of 70 alleles of somatic mutations of EGFR, KRAS, BRAF and PIK3CA can be detected simultaneously in one reaction from one formalin-fixed and paraffin-embedded (FFPE) slide within one day. Paraffin 158-166 epidermal growth factor receptor Homo sapiens 55-59 20207772-6 2010 RESULTS: The digital array detected and quantified rare gefitinib/erlotinib-sensitizing EGFR mutations (0.02%-9.26% abundance) that were present in formalin-fixed, paraffin-embedded samples of early-stage resectable lung tumors without an associated increase in gene copy number. Paraffin 164-172 epidermal growth factor receptor Homo sapiens 88-92 19525925-12 2009 The high concordance rate of silver enhanced in situ hybridization and fluorescence in situ hybridization for the detection of EGFR amplification in paraffin-embedded glioblastomas samples demonstrates that silver enhanced in situ hybridization is a valid and attractive alternative to fluorescence in situ hybridization. Paraffin 149-157 epidermal growth factor receptor Homo sapiens 127-131 20093389-0 2010 Mutation detection of epidermal growth factor receptor and KRAS genes using the smart amplification process version 2 from formalin-fixed, paraffin-embedded lung cancer tissue. Paraffin 139-147 epidermal growth factor receptor Homo sapiens 22-54 19317849-3 2009 We analysed EGFR expression using immunohistochemistry on formalin-fixed, paraffin embedded surgical specimens of all patients. Paraffin 74-82 epidermal growth factor receptor Homo sapiens 12-16 19212684-5 2009 Using tissue microarray of samples from 47 paraffin-embedded osteosarcoma cases, 26, 17, 20 and 12 cases showed positive immunostaining of EGFR, Stat-3, survivin and Erk, respectively. Paraffin 43-51 epidermal growth factor receptor Homo sapiens 139-143 19509244-3 2009 We determined the expression of epidermal growth factor receptor (EGFR) and activated mammalian target of rapamycin (p-mTOR) in paraffin-embedded surgical specimens of BTA (n = 89) by immunohistochemistry. Paraffin 128-136 epidermal growth factor receptor Homo sapiens 32-64 19063875-3 2009 We attempted to establish a new method for the detection of EGFR gene mutations with the use of paraffin-embedded samples. Paraffin 96-104 epidermal growth factor receptor Homo sapiens 60-64 19214113-0 2009 A novel proximity assay for the detection of proteins and protein complexes: quantitation of HER1 and HER2 total protein expression and homodimerization in formalin-fixed, paraffin-embedded cell lines and breast cancer tissue. Paraffin 172-180 epidermal growth factor receptor Homo sapiens 93-97 18660793-6 2009 Immunostaining for epidermal growth factor receptor (EGFR) was performed on paraffin-embedded tissue sections containing tumor from these specimens, using the avidin-biotin-peroxidase method. Paraffin 76-84 epidermal growth factor receptor Homo sapiens 53-57 17183836-1 2006 The present study provides an analysis of immunohistochemical expression and localization of epidermal growth factor receptor (EGFR) in formalin fixed paraffin embedded specimens of prostate. Paraffin 151-159 epidermal growth factor receptor Homo sapiens 93-125 16854613-2 2007 EGFR expression was examined immunohistochemically on a tissue microarray (TMA) of paraffin embedded tissue specimens from 109 patients who underwent surgical treatment for squamous cell carcinoma of the oral cavity and oropharynx in the period between 1980 and 1997. Paraffin 83-91 epidermal growth factor receptor Homo sapiens 0-4 17183836-1 2006 The present study provides an analysis of immunohistochemical expression and localization of epidermal growth factor receptor (EGFR) in formalin fixed paraffin embedded specimens of prostate. Paraffin 151-159 epidermal growth factor receptor Homo sapiens 127-131 16839708-4 2006 EGFR expression in pretreatment paraffin-embedded tumor biopsy specimens was assessed by immunohistochemistry. Paraffin 32-40 epidermal growth factor receptor Homo sapiens 0-4 16685386-9 2006 The current study suggests that DNA extraction with the DEXPAT kit followed by nested PCR is a simple and reliable technique for analyzing the EGFR gene status with paraffin-embedded samples. Paraffin 165-173 epidermal growth factor receptor Homo sapiens 143-147 16825506-3 2006 We therefore developed a new assay for detecting EGFR mutation using only a paraffin section of a small biopsy specimen. Paraffin 76-84 epidermal growth factor receptor Homo sapiens 49-53 16795991-1 2006 BACKGROUND/AIMS: To evaluate epidermal growth factor receptor (EGFR) gene status in pancreatic ductal adenocarcinoma correlating the results to protein expression and clinicopathological features METHODOLOGY: Using tissue microarray technology (TMArrayer 100), fifty (n = 50) paraffin-embedded tissue samples of histologically-confirmed primary tumors were cored twice at a diameter of 1 mm and re-embedded into the final recipient block. Paraffin 276-284 epidermal growth factor receptor Homo sapiens 63-67 16467085-9 2006 RESULTS: EGFR mutations were detected in 16 of 61 fresh frozen tumor specimens, 24 of 91 dissected formalin-fixed, paraffin-embedded tumor specimens, and 11 of 26 undissected formalin-fixed, paraffin-embedded tumor specimens. Paraffin 115-123 epidermal growth factor receptor Homo sapiens 9-13 16152581-4 2006 EGFR mutation was assessed from DNA of 63 paraffin-embedded small needle biopsy/aspiration specimens from 62 patients with NSCLC treated with gefitinib. Paraffin 42-50 epidermal growth factor receptor Homo sapiens 0-4 16152581-11 2006 In conclusion, EGFR mutation analysis is feasible in needle biopsy/aspiration paraffin-fixed specimens. Paraffin 78-86 epidermal growth factor receptor Homo sapiens 15-19 16467085-9 2006 RESULTS: EGFR mutations were detected in 16 of 61 fresh frozen tumor specimens, 24 of 91 dissected formalin-fixed, paraffin-embedded tumor specimens, and 11 of 26 undissected formalin-fixed, paraffin-embedded tumor specimens. Paraffin 191-199 epidermal growth factor receptor Homo sapiens 9-13 16324192-2 2005 METHODS AND RESULTS: Fluorescence in situ hybridization of the EGFR gene and CEP7 was carried out on paraffin-embedded material from 48 rectal carcinomas combined with immunohistochemical detection of EGFR with a polymer detection kit. Paraffin 101-109 epidermal growth factor receptor Homo sapiens 63-67 17189989-0 2006 Protocol modifications influence the result of EGF receptor immunodetection by EGFR pharmDx in paraffin-embedded cancer tissues. Paraffin 95-103 epidermal growth factor receptor Homo sapiens 47-59 17189989-0 2006 Protocol modifications influence the result of EGF receptor immunodetection by EGFR pharmDx in paraffin-embedded cancer tissues. Paraffin 95-103 epidermal growth factor receptor Homo sapiens 79-83 17189989-4 2006 The golden standard for the determination of EGFR protein expression in paraffin-embedded cancer tissues is the EGFR pharmDxTM kit. Paraffin 72-80 epidermal growth factor receptor Homo sapiens 45-49 16012707-5 2005 By performing immunohistochemistry on paraffin-embedded tissue sections, we found that ptyr-845 EGFR was significantly co-expressed with Her-2/neu and ptyr-1248 Her-2/neu (p=0.043 and p=0.040, respectively), while ptyr-1173 EGFR was only correlated to Her-2/neu expression (p=0.042). Paraffin 38-46 epidermal growth factor receptor Homo sapiens 96-100 15956035-3 2005 The mutation analysis of the EGFR kinase domain was performed for 30 patients using paraffin-embedded tumor tissue. Paraffin 84-92 epidermal growth factor receptor Homo sapiens 29-33 16115926-14 2005 CONCLUSION: The AQUA system provides a continuous measurement of EGFR on paraffin-embedded tissue and was able to reveal the association between EGFR expression and outcome expected from the biological role of EGFR. Paraffin 73-81 epidermal growth factor receptor Homo sapiens 65-69 16115926-14 2005 CONCLUSION: The AQUA system provides a continuous measurement of EGFR on paraffin-embedded tissue and was able to reveal the association between EGFR expression and outcome expected from the biological role of EGFR. Paraffin 73-81 epidermal growth factor receptor Homo sapiens 145-149 16115926-14 2005 CONCLUSION: The AQUA system provides a continuous measurement of EGFR on paraffin-embedded tissue and was able to reveal the association between EGFR expression and outcome expected from the biological role of EGFR. Paraffin 73-81 epidermal growth factor receptor Homo sapiens 145-149 15875085-4 2005 The expression of Ki-67 and EGFR protein was visualized on formalin fixed, paraffin embedded sections using immunohistochemistry (IHC). Paraffin 75-83 epidermal growth factor receptor Homo sapiens 28-32 15897572-3 2005 EXPERIMENTAL DESIGN: EGFR mutations in exons 18 to 21 were analyzed by DNA sequencing of paraffin-embedded tumor tissues from gefitinib-treated NSCLC patients. Paraffin 89-97 epidermal growth factor receptor Homo sapiens 21-25 15943037-3 2005 The aim of this study was to assess EGFR expression in human high-grade astrocytomas by means of immunohistochemistry on formalin-fixed, paraffin-embedded sections and to compare these findings with the results of our previous study on frozen sections from these tumours, in which we found about 60% EGFR positivity (10). Paraffin 137-145 epidermal growth factor receptor Homo sapiens 36-40 15623594-3 2004 EXPERIMENTAL DESIGN: Mutational analysis of the kinase domain of EGFR coding sequence was done on 101 fresh frozen tumor tissues from patients without prior gefitinib treatment and 16 paraffin-embedded tumor tissues from patients treated with gefitinib. Paraffin 184-192 epidermal growth factor receptor Homo sapiens 65-69 11758715-1 2001 The expression of EGFR family and steroid hormone receptors was examined in a series of 40 cases of pure ductal carcinoma in situ (DCIS) of the breast by immunohistochemical staining of paraffin-embedded sections. Paraffin 186-194 epidermal growth factor receptor Homo sapiens 18-22 15253134-4 2004 The EGFR expression was visualized in formalin-fixed, paraffin-embedded sections, using immunohistochemistry. Paraffin 54-62 epidermal growth factor receptor Homo sapiens 4-8 12375051-5 2002 We, therefore, used a double differential polymerase chain reaction (ddPCR) for determination of the amplification/deletion profiles of erbB-1, -2, -3 and -4 genes in formalin-fixed, paraffin embedded (FFPE) specimens of human PHEOs. Paraffin 183-191 epidermal growth factor receptor Homo sapiens 136-157 15208356-0 2004 Immunohistochemical detection of EGFR in paraffin-embedded tumor tissues: variation in staining intensity due to choice of fixative and storage time of tissue sections. Paraffin 41-49 epidermal growth factor receptor Homo sapiens 33-37 15073595-2 2004 EGFR expression was investigated in paraffin sections of resection specimens of 89 gastric carcinomas from Mexican Mestizo patients using standardized immunohistochemistry with antigen retrieval (Dako EGFRpharmDx assay detection system). Paraffin 36-44 epidermal growth factor receptor Homo sapiens 0-4 15163002-3 2004 EGFR gene amplification and chromosome 7 aneuploidy was detected in 34 formalin-fixed, paraffin-embedded benign and malignant gliomas by chromogenic in situ hybridization (CISH) using digoxigenin-labeled EGFR and biotin-labeled chromosome 7 centromeric probes. Paraffin 87-95 epidermal growth factor receptor Homo sapiens 0-4 7781795-4 1995 To this end, the expression of EGF-R mRNA and protein by non-radioactive in-situ hybridization and immunohistochemistry, respectively, were determined on histological preparations of formalin-fixed paraffin-embedded colorectal surgical specimens. Paraffin 198-206 epidermal growth factor receptor Homo sapiens 31-36 10226061-5 1999 Immunohistochemical analysis of resin- or paraffin-embedded sections from surgical specimens of bronchial mucosa revealed strong membrane staining for EGFR within the bronchial epithelium; this was particularly evident between basal cells and the basal aspect of columnar cells. Paraffin 42-50 epidermal growth factor receptor Homo sapiens 151-155 9252718-2 1997 We determined EGF-R expression by an immunohistochemical assay on paraffin-embedded primary breast cancer tissue by means of the anti EGF-R mab E30 (Merck, FRG) and streptavidin-peroxidase technique. Paraffin 66-74 epidermal growth factor receptor Homo sapiens 14-19 8598357-2 1996 METHODS AND MATERIALS: Epidermal growth factor receptor protein expression was investigated immunohistochemically in formalin-fixed, paraffin-embedded surgical specimens of 55 glioblastoma multiforme, 14 anaplastic astrocytoma, and 2 astrocytomas given definitive irradiation. Paraffin 133-141 epidermal growth factor receptor Homo sapiens 23-55 11102891-4 2000 Immunoreactivity for EGF-R, TGF-alpha, p53 and c-erbB-2 with paraffin-embedded carcinoma tissue was investigated using labeled streptavidin-biotin methods. Paraffin 61-69 epidermal growth factor receptor Homo sapiens 21-26 8758275-2 1996 METHODS: Expression of EGFR oncoprotein was studied by immunohistochemistry in paraffin-embedded tumor tissues from 28 patients with endometrioid carcinoma of the ovary. Paraffin 79-87 epidermal growth factor receptor Homo sapiens 23-27 7779717-0 1995 Immunohistochemical assay for epidermal growth factor receptor on paraffin-embedded sections: validation against ligand-binding assay and clinical relevance in breast cancer. Paraffin 66-74 epidermal growth factor receptor Homo sapiens 30-62 7718322-1 1995 Epidermal growth factor receptor (EGFr) levels were evaluated in paraffin-embedded tumour specimens of non-small cell lung cancer (NSCLC) from 176 patients who underwent surgical resection. Paraffin 65-73 epidermal growth factor receptor Homo sapiens 34-38 7607382-0 1995 [Immunohistochemical detection of epidermal growth factor receptor (EGF-R) in paraffin sections of breast carcinoma tissue: correlation and clinical significance]. Paraffin 78-86 epidermal growth factor receptor Homo sapiens 34-66 7607382-0 1995 [Immunohistochemical detection of epidermal growth factor receptor (EGF-R) in paraffin sections of breast carcinoma tissue: correlation and clinical significance]. Paraffin 78-86 epidermal growth factor receptor Homo sapiens 68-73 7607382-4 1995 Studies with immunohistochemical analyses of EGF-R overexpression in formalin-fixed, paraffin-embedded tumour samples are rare. Paraffin 85-93 epidermal growth factor receptor Homo sapiens 45-50 7607382-5 1995 In a retrospective study, we examined the clinical data from 142 patients and the EGF-R expression in their formalin-fixed, paraffin-embedded tumour samples. Paraffin 124-132 epidermal growth factor receptor Homo sapiens 82-87 7723282-6 1995 EGF-R expression was detected by immunohistochemistry on paraffin-embedded tumor sections of the same specimens. Paraffin 57-65 epidermal growth factor receptor Homo sapiens 0-5 7607382-12 1995 In this study, the determination of EGF-R in formalin-fixed, paraffin-embedded tumour samples proved feasible. Paraffin 61-69 epidermal growth factor receptor Homo sapiens 36-41 7715901-2 1995 EGFR gene amplification was examined by slot-blot hybridization using DNA extracted from formalin-fixed, paraffin-embedded tissues. Paraffin 105-113 epidermal growth factor receptor Homo sapiens 0-4 7937720-10 1994 Three of eleven cases, all of which expressed EGFR, were felt to show EGFR gene amplification using a modification of the differential polymerase chain reaction on archival, formalin-fixed, paraffin-embedded tissue. Paraffin 190-198 epidermal growth factor receptor Homo sapiens 70-74 8236825-0 1993 Epidermal growth factor receptor expression in squamous cell lung carcinomas: an immunohistochemical and gene analysis in formalin-fixed, paraffin-embedded material. Paraffin 138-146 epidermal growth factor receptor Homo sapiens 0-32 8293402-5 1994 Assessment of EGFR overexpression was done at the protein level by the use of an anti-EGFR polyclonal antibody that reacts with the cytoplasmic membrane glycoprotein receptor in paraffin-embedded tissues. Paraffin 178-186 epidermal growth factor receptor Homo sapiens 14-18 8293402-5 1994 Assessment of EGFR overexpression was done at the protein level by the use of an anti-EGFR polyclonal antibody that reacts with the cytoplasmic membrane glycoprotein receptor in paraffin-embedded tissues. Paraffin 178-186 epidermal growth factor receptor Homo sapiens 86-90 8439966-0 1993 A rapid colorimetric in situ messenger RNA hybridization technique for analysis of epidermal growth factor receptor in paraffin-embedded surgical specimens of human colon carcinomas. Paraffin 119-127 epidermal growth factor receptor Homo sapiens 83-115 8439966-1 1993 We have developed a rapid colorimetric in situ mRNA hybridization procedure to analyze epidermal growth factor receptor (EGF-R) transcripts in paraffin-embedded surgical specimens of human colon carcinomas. Paraffin 143-151 epidermal growth factor receptor Homo sapiens 87-119 8439966-1 1993 We have developed a rapid colorimetric in situ mRNA hybridization procedure to analyze epidermal growth factor receptor (EGF-R) transcripts in paraffin-embedded surgical specimens of human colon carcinomas. Paraffin 143-151 epidermal growth factor receptor Homo sapiens 121-126 8439966-6 1993 In situ hybridization of paraffin-embedded sections of primary human colon carcinoma and metastases from liver and lymph node revealed cell-specific staining with EGF-R antisense oligonucleotide probes that correlated directly with Northern blot and immunohistochemistry analyses. Paraffin 25-33 epidermal growth factor receptor Homo sapiens 163-168 8321742-2 1993 Immunohistochemical study for epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) was performed on paraffin-embedded tissue specimens from 39 colorectal and 24 gastric carcinomas. Paraffin 121-129 epidermal growth factor receptor Homo sapiens 98-102 8236825-5 1993 In addition, analysis of the EGFr gene was carried out by Southern blotting and DPCR on paraffin extracted DNA from the same carcinoma cases. Paraffin 88-96 epidermal growth factor receptor Homo sapiens 29-33 2025606-8 1991 We conclude that the expression EGF-R can be detected reliably by the F4 antibody in both the fresh frozen and formalin fixed, paraffin embedded material and could be useful in assessing the clinical importance of EGF-R in archival tumour material. Paraffin 127-135 epidermal growth factor receptor Homo sapiens 32-37 1681040-0 1991 Immunohistochemical detection of the epidermal growth factor receptor in paraffin-embedded human tissues. Paraffin 73-81 epidermal growth factor receptor Homo sapiens 37-69 1681040-1 1991 Expression of the epidermal growth factor (EGF) receptor was evaluated by immunohistochemical staining of formalin-fixed, paraffin-embedded tumour tissues employing two antibodies raised to short synthetic peptides from the cytoplasmic domain of the molecule. Paraffin 122-130 epidermal growth factor receptor Homo sapiens 18-56 21584551-2 1992 With a monoclonal antibody for EGF-receptor and avidin-biotin-peroxidase complex (ABC), the expression of EGF-receptor was evaluated in paraffin-embedded sections. Paraffin 136-144 epidermal growth factor receptor Homo sapiens 106-118 33816221-4 2021 Case Study: We report about SCCT that occurred 45 months after the initial diagnosis of ADC in an East Asian never-smoker woman with advanced-stage EGFR Del-19-mutant lung ADC treated with combined chemoradiotherapy before the era of insurance coverage for EGFR-TKIs in this country and subsequently gefitinib; deletion at codon 746-750 in exon 19 of the EGFR gene was ascertained in the original formalin-fixed paraffin-embedded lung biopsy tissue. Paraffin 412-420 epidermal growth factor receptor Homo sapiens 148-152 2372497-1 1990 Relationship of EGF-R with histology and survival using fixed paraffin embedded tissue and the F4, monoclonal antibody. Paraffin 62-70 epidermal growth factor receptor Homo sapiens 16-21 2372497-2 1990 The expression of epidermal growth factor receptor (EGF-R) in 34 formalin fixed paraffin embedded specimens of malignant mesothelioma was examined using the F4 antibody. Paraffin 80-88 epidermal growth factor receptor Homo sapiens 18-50 2372497-2 1990 The expression of epidermal growth factor receptor (EGF-R) in 34 formalin fixed paraffin embedded specimens of malignant mesothelioma was examined using the F4 antibody. Paraffin 80-88 epidermal growth factor receptor Homo sapiens 52-57 1698166-4 1990 The monoclonal antibodies to c-erbB-2 and EGF-R immunostained subpopulations of tumour cells on sections of formalin-fixed, paraffin wax embedded human infiltrating and invasive ductal carcinomas of breast. Paraffin 124-136 epidermal growth factor receptor Homo sapiens 42-47 34344387-3 2021 This study aimed to validate the Idylla platform for the detection of EGFR/KRAS mutations in cytological NSCLC samples prepared as cytoblocks using AGAR and paraffin embedding. Paraffin 158-166 epidermal growth factor receptor Homo sapiens 71-75 34400544-3 2021 The Idylla EGFR mutation test (IEMT) has been specifically designed to process formalin-fixed paraffin-embedded sections without requiring preliminary DNA extraction.This study aims to evaluate the diagnostic accuracy of IEMT when used to analyse archival histopathology material. Paraffin 94-102 epidermal growth factor receptor Homo sapiens 11-15 2544220-3 1989 EGF-R status was assessed on routinely embedded paraffin sections with an antibody to the cytoplasmic domain of the tumour (F4 antibody). Paraffin 48-56 epidermal growth factor receptor Homo sapiens 0-5 35228756-11 2022 Furthermore, the performance of Idylla EGFR Mutation Test and Qiagen Therascreen EGFR RGQ PCR on archived paraffin sections of limited quantities is available with the high agreement of results. Paraffin 108-116 epidermal growth factor receptor Homo sapiens 83-87 35545361-5 2022 This study aims to evaluate the consistency of EGFR mutant gene expression between the 2 methods, and to explore a reliable way for preserving cytological data and making full use of cytological archival data via cell HE staining smear and cell paraffin section. Paraffin 245-253 epidermal growth factor receptor Homo sapiens 47-51 35545361-10 2022 EGFR gene mutation was detected by amplification refractory mutation system-polymerase chain reaction in 57 paired cell HE staining smears and cell paraffin sections. Paraffin 148-156 epidermal growth factor receptor Homo sapiens 0-4 33597223-4 2022 The automated Idylla epidermal growth factor receptor (EGFR) mutation test has been developed to rapidly process formalin-fixed paraffin-embedded (FFPE) pathologic material, without previous DNA extraction. Paraffin 128-136 epidermal growth factor receptor Homo sapiens 21-53 3668732-4 1987 Mab F4 is the first antibody to the EGF receptor to show reactivity in paraffin sections. Paraffin 71-79 epidermal growth factor receptor Homo sapiens 36-48 33597223-4 2022 The automated Idylla epidermal growth factor receptor (EGFR) mutation test has been developed to rapidly process formalin-fixed paraffin-embedded (FFPE) pathologic material, without previous DNA extraction. Paraffin 128-136 epidermal growth factor receptor Homo sapiens 55-59 32150626-4 2020 EGFR expression was assessed on the archival Formalin Fixed Paraffin Embedded (FFPE) blocks using immunohistochemical staining technique. Paraffin 60-68 epidermal growth factor receptor Homo sapiens 0-4 33357312-5 2020 Among them, 132 patients with relatively complete clinical data and with EGFR gene mutation detection of paraffin-embedded pleural effusion sediment section according to the established quality control standard were included. Paraffin 105-113 epidermal growth factor receptor Homo sapiens 73-77 32245434-0 2020 Clinical performance evaluation of the Idylla EGFR Mutation Test on formalin-fixed paraffin-embedded tissue of non-small cell lung cancer. Paraffin 84-92 epidermal growth factor receptor Homo sapiens 47-51 32245434-3 2020 The Idylla EGFR Mutation Test is a simple, fast and reliable method designed for the detection of EGFR mutations from formalin-fixed paraffin-embedded samples. Paraffin 134-142 epidermal growth factor receptor Homo sapiens 12-16 32245434-3 2020 The Idylla EGFR Mutation Test is a simple, fast and reliable method designed for the detection of EGFR mutations from formalin-fixed paraffin-embedded samples. Paraffin 134-142 epidermal growth factor receptor Homo sapiens 99-103 32245434-5 2020 METHODS: EGFR mutational status was determined on 132 archived formalin-fixed paraffin-embedded tissue sections with Idylla technology. Paraffin 78-86 epidermal growth factor receptor Homo sapiens 9-13 31859115-0 2020 Comparison detection methods for EGFR in formalin-fixed paraffin-embedded tissues of patients with NSCLC. Paraffin 56-64 epidermal growth factor receptor Homo sapiens 33-37 32166668-2 2020 Paraffin-embedded sections of NSCLC tissues from 399 NSCLC patients diagnosed in Renmin Hospital of Wuhan University were collected, 103 of them were detected for exons 18-21 mutation of EGFR by Sanger sequencing method, 296 cases were detected for exons 18-21 mutation by ARMS-PCR method. Paraffin 0-8 epidermal growth factor receptor Homo sapiens 187-191 32025485-2 2020 For NSCLC patients, EGFR mutation testing is performed using the commercial Cobas EGFR Mutation test v2.0, which can be used to analyze both formalin-fixed, paraffin-embedded (FFPE) tissue (FFPE test, or FT) and plasma samples (plasma test, or PT). Paraffin 157-165 epidermal growth factor receptor Homo sapiens 20-24 31110050-2 2019 AIM: The rapid and fully automated Idylla EGFR Mutation Assay has been specifically designed to process formalin-fixed, paraffin-embedded sections without requiring preliminary DNA extraction. Paraffin 120-128 epidermal growth factor receptor Homo sapiens 42-46 31870098-0 2019 Cell Free Tumoral DNA Versus Paraffin Block Epidermal Growth Factor Receptor Mutation Detection in Patients with Non-Small Cell Lung Cancer. Paraffin 29-37 epidermal growth factor receptor Homo sapiens 44-76 31407509-0 2019 EGFR T790M detection in formalin-fixed paraffin-embedded tissues of patients with lung cancer using RNA-based in situ hybridization: A preliminary feasibility study. Paraffin 39-47 epidermal growth factor receptor Homo sapiens 0-4 31445213-0 2019 Multi-Center Evaluation of the Fully Automated PCR-Based Idylla EGFR Mutation Assay on Formalin-Fixed, Paraffin-Embedded Tissue of Human Lung Cancer. Paraffin 103-111 epidermal growth factor receptor Homo sapiens 64-68 31127887-0 2019 Detection of Epidermal Growth Factor Receptor (EGFR) Gene Mutation in Formalin Fixed Paraffin Embedded Tissue by Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) in Non-Small Cell Lung Cancer in the Northeastern Region of Thailand Background: The use of targeted specific genes in therapeutic and treatment decisions has been considered forlung cancer. Paraffin 85-93 epidermal growth factor receptor Homo sapiens 13-45 31258360-6 2019 The EGFR protein levels were detected with immunohistochemistry methods from the sections of the patients" paraffin blocks. Paraffin 107-115 epidermal growth factor receptor Homo sapiens 4-8 31127887-0 2019 Detection of Epidermal Growth Factor Receptor (EGFR) Gene Mutation in Formalin Fixed Paraffin Embedded Tissue by Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) in Non-Small Cell Lung Cancer in the Northeastern Region of Thailand Background: The use of targeted specific genes in therapeutic and treatment decisions has been considered forlung cancer. Paraffin 85-93 epidermal growth factor receptor Homo sapiens 47-51 30233874-8 2018 Methods: Mutations in EGFR gene were examined using real-time PCR technique in 1,108 formalin-fixed paraffin-embedded (FFPE) tissues, 398 FFPE cell-blocks and 470 cytological specimens of NSCLC. Paraffin 100-108 epidermal growth factor receptor Homo sapiens 22-26 30243897-4 2018 It is based on EGFR screening methods using paraffin-embedded tumour specimen to select patients eligible for treatment. Paraffin 44-52 epidermal growth factor receptor Homo sapiens 15-19