PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31098781-0 2019 ASO Author Reflections: Precise p53 Analysis in Formalin-Fixed Paraffin-Embedded Specimens Can Predict Head and Neck Squamous Cell Carcinoma Outcomes. Paraffin 63-71 tumor protein p53 Homo sapiens 32-35 33631963-6 2021 Next-generation sequencing (NGS) was performed on formalin-fixed paraffin-embedded tumor tissue, revealing one mutation in exon 5, TP53: p.A159 V, which may be associated with chemo-resistance. Paraffin 65-73 tumor protein p53 Homo sapiens 131-135 32451170-4 2020 MATERIALS AND METHODS: TP53 was PCR amplified using the genomic DNA extracted from peripheral blood mononuclear cells and formalin-fixed, paraffin-embedded tissue sections from the tumor site of the patient, and was sequenced. Paraffin 138-146 tumor protein p53 Homo sapiens 23-27 27832134-0 2016 Comparison of the QuantiGene 2.0 Assay and Real-Time RT-PCR in the Detection of p53 Isoform mRNA Expression in Formalin-Fixed Paraffin-Embedded Tissues- A Preliminary Study. Paraffin 126-134 tumor protein p53 Homo sapiens 80-83 30211098-7 2018 Expression of mutant p53 protein from NPC tissue paraffin block with immunohistochemical cracking technique was using monoclonal rabbit Anti Human p53 clone 318-6-11 (Dako, North America, Inc., 6392 Via Real Carpinteria, CA 93013 USA), microscope light binoculars was assessed visually by an Anatomical Pathology Consultant. Paraffin 49-57 tumor protein p53 Homo sapiens 21-24 29774995-2 2017 Method:Immunohistochemical staining of MCT-1 and p53 was carried out in 41 cases of laryngeal squamous cell carcinoma embedded in paraffin, and the relationship between positive expression and clinicopathological data was evaluated. Paraffin 130-138 tumor protein p53 Homo sapiens 49-52 28003030-6 2016 Immunohistochemical staining for p53 was performed on paraffin-embedded 5-mum sections using mouse anti-human tumor protein p53 monoclonal antibody (DO-7, Dako, Denmark). Paraffin 54-62 tumor protein p53 Homo sapiens 33-36 27891503-4 2016 In this study, we have optimized the protocol for Sanger sequencing of TP53 using DNA extracted from archival paraffin-embedded biopsies. Paraffin 110-118 tumor protein p53 Homo sapiens 71-75 28421464-6 2017 Somatic gene sequencing revealed a novel TP53 mutation in DNA extracted from paraffin-embedded tissue, a deletion of 8bp in exon 8 (c.811_818del8; GAGGTGCG/-) in homo or hemizygosis causing a subsequent frameshift and premature stop codon at position 302. Paraffin 77-85 tumor protein p53 Homo sapiens 41-45 28471195-13 2017 CONCLUSION: During development of PLA method for detection of protein complexes between p53 family members we detected interactions of p53 and p63 with T-antigen and mut-p53 and DeltaNp63 with TAp73 tumour suppressor in tumour cell lines and p53 tetramers in paraffin sections of colorectal cancer tissue. Paraffin 259-267 tumor protein p53 Homo sapiens 88-91 28471195-13 2017 CONCLUSION: During development of PLA method for detection of protein complexes between p53 family members we detected interactions of p53 and p63 with T-antigen and mut-p53 and DeltaNp63 with TAp73 tumour suppressor in tumour cell lines and p53 tetramers in paraffin sections of colorectal cancer tissue. Paraffin 259-267 tumor protein p53 Homo sapiens 135-138 28471195-13 2017 CONCLUSION: During development of PLA method for detection of protein complexes between p53 family members we detected interactions of p53 and p63 with T-antigen and mut-p53 and DeltaNp63 with TAp73 tumour suppressor in tumour cell lines and p53 tetramers in paraffin sections of colorectal cancer tissue. Paraffin 259-267 tumor protein p53 Homo sapiens 135-138 28471195-13 2017 CONCLUSION: During development of PLA method for detection of protein complexes between p53 family members we detected interactions of p53 and p63 with T-antigen and mut-p53 and DeltaNp63 with TAp73 tumour suppressor in tumour cell lines and p53 tetramers in paraffin sections of colorectal cancer tissue. Paraffin 259-267 tumor protein p53 Homo sapiens 135-138 27997533-5 2016 Patient-specific TP53 assays were developed for 31 unique mutations identified in formalin-fixed paraffin-embedded tumour DNA from these patients. Paraffin 97-105 tumor protein p53 Homo sapiens 17-21 27832134-4 2016 However, there are serious limitations when detecting p53 isoforms using this method, particularly for formalin-fixed paraffin-embedded (FFPE) tissues. Paraffin 118-126 tumor protein p53 Homo sapiens 54-57 27780269-7 2016 Next, we have measured the p53 expression in the paraffin embedded blocks prepared from bone marrow biopsy, using immunohistochemistry, and the expression levels correlated with VB9 and VB12 levels. Paraffin 49-57 tumor protein p53 Homo sapiens 27-30 27369359-2 2016 Paraffin tumors of all clinical subtypes from 1661 patients with operable breast cancer who were treated within 4 adjuvant trials with anthracycline-taxanes chemotherapy were informative for TP53 and PIK3CA mutation status (semiconductor sequencing genotyping) and for stromal TILs density. Paraffin 0-8 tumor protein p53 Homo sapiens 191-195 27323551-6 2016 Immuno-histochemical staining for P53 performed on paraffin blocks and quantified with 12- point weighted score proposed by W.Y chan. Paraffin 51-59 tumor protein p53 Homo sapiens 34-37 24716981-4 2014 Expression of p53 protein was evaluated immunohistochemically in sections of paraffin- embedded tissue. Paraffin 77-85 tumor protein p53 Homo sapiens 14-17 25473754-4 2015 Immunohistochemistry for p53 was performed on all paraffin blocks of fallopian tube and non-neoplastic endometrium. Paraffin 50-58 tumor protein p53 Homo sapiens 25-28 24368645-5 2014 Mutational analysis of p53 and EGFR was performed on DNA extracted from paraffin-embedded tumors. Paraffin 72-80 tumor protein p53 Homo sapiens 23-26 25135238-7 2014 The TP53 genotype was assessed from paraffin-embedded diagnostic tumor biopsies using a standardized gene-specific TP53 sequencing protocol (mark53 kit; mark53 Ltd, Vienna, Austria). Paraffin 36-44 tumor protein p53 Homo sapiens 4-8 25202347-6 2014 In addition, immunohistochemistry (IHC) was performed to detect the p53 expression level in specimens from 118 paraffin-embedded cancerous tissues. Paraffin 111-119 tumor protein p53 Homo sapiens 68-71 24112103-3 2014 METHODS: TP53 mutations were determined with direct sequencing on paraffin-embedded carcinoma tissue from 31 young patients and compared with two older age OSCC reference groups: one from the same institute (N = 87) and an independent one (N = 675). Paraffin 66-74 tumor protein p53 Homo sapiens 9-13 23073516-1 2014 BACKGROUND: Investigation of p53 immunoreactivity in formalin-fixed paraffin-embedded tissues of normal renal tissue and renal cell carcinoma with respect to histopathologic subtype and nuclear grade of RCC. Paraffin 68-76 tumor protein p53 Homo sapiens 29-32 23168703-6 2012 Mutational analysis of TP53 was done in DNA extracted from formalin fixed paraffin embedded tissue of 80 CaP and 24 BPH cases. Paraffin 74-82 tumor protein p53 Homo sapiens 23-27 24173110-4 2014 Three SNPs in the p53 pathway were identified using the Sanger sequencing method from retrospectively collected paraffin-embedded biopsy specimens. Paraffin 112-120 tumor protein p53 Homo sapiens 18-21 24433772-2 2013 METHODS: The expression of Gli1, MDM2 and p53 proteins in 57 paired paraffin embedded pancreatic ductal adenocarcinoma (PDAC) specimens and adjacent non-cancerous pancreatic tissues was detected by immunohistochemistry. Paraffin 68-76 tumor protein p53 Homo sapiens 42-45 23663243-5 2013 The p53 codon 72 SNP was identified from retrospectively collected paraffin-embedded biopsy specimens using Sanger sequencing. Paraffin 67-75 tumor protein p53 Homo sapiens 4-7 23360043-4 2012 Immunohistochemical staining for p53 was performed on formalin fixed paraffin embedded tissue sections with appropriate positive and negative control. Paraffin 69-77 tumor protein p53 Homo sapiens 33-36 22534715-7 2012 Interestingly, direct DNA sequencing of the paraffin-embedded tumor sample identified a novel R248Q mutation in the TP53 gene. Paraffin 44-52 tumor protein p53 Homo sapiens 116-120 22858706-5 2012 Paraffin-embebeded sections were immunostained for p53 and p21WAF1. Paraffin 0-8 tumor protein p53 Homo sapiens 51-54 21933600-5 2011 METHODS: The expression of P53 protein and MDM-2 protein was immunohistochemically detected using monoclonal antibodies in 242 paraffin embedded tissues, including 30 normal brain tissues from patients with craniocerebral injury and 212 tissues from patients with primary glioma (grade I - II group: 5 cases of grade I, 119 cases of grade II; and grade III--IV group: 53 cases of grade III, and 35 cases of grade IV). Paraffin 127-135 tumor protein p53 Homo sapiens 27-30 22391175-2 2012 Using fluorescence in situ hybridization (FISH), the P53 gene was detected in paraffin-embedded tonsil tissues from 50 cases of DLBCL, while all the peripheral blood of patients was collected for detecting P53 protein in serum by using enzyme-linked immunosorbent assay (ELISA). Paraffin 78-86 tumor protein p53 Homo sapiens 53-56 22242209-2 2011 We assessed the immunohistochemical expression of p53 and P-gp using formalin-fixed, paraffin-embedded specimens in 108 patients diagnosed with de novo DLBC. Paraffin 85-93 tumor protein p53 Homo sapiens 50-53 21693655-3 2011 PATIENTS AND METHODS: Epithelial p53 expression was evaluated using two immunohistochemical antibodies (DO7 and 1801) in formalin-fixed, paraffin-embedded tissue from patients with node-positive breast cancer who were randomized to four cycles of cyclophosphamide and one of three doses of doxorubicin (60, 75, or 90 mg/m(2); AC) and to receive four subsequent cycles of paclitaxel (T) or not. Paraffin 137-145 tumor protein p53 Homo sapiens 33-36 21866459-3 2011 MRP4 and P53 protein expression in the paraffin-embedded specimen were detected by immunohistochemistry. Paraffin 39-47 tumor protein p53 Homo sapiens 9-12 21598493-3 2011 Different particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein simultaneously in paraffin-embedded tissue section of human tongue squamous cell carcinoma under fluorescent microscope. Paraffin 127-135 tumor protein p53 Homo sapiens 79-82 21598493-6 2011 P53 protein and Bcl-2 protein can be combined with different particle size quantum dots fluorescent probes respectively in the same paraffin-embedded tissue section of human tongue squamous cell carcinoma and two kinds of fluorescene can be observed. Paraffin 132-140 tumor protein p53 Homo sapiens 0-3 22442608-2 2011 MATERIALS AND METHODS: The standard immunohistochemical method along with MIB-1 and DO-7; DAKO antibodies was used to study the expression of p53 and ki-67 in paraffin-embedded tissue specimens. Paraffin 159-167 tumor protein p53 Homo sapiens 142-145 20010306-3 2010 Mutation analysis of TP53 Exons 4 to 9 on DNA from formalin-fixed, paraffin-embedded specimens was performed by bidirectional DNA sequencing. Paraffin 67-75 tumor protein p53 Homo sapiens 21-25 20461443-3 2010 We aimed to comprehensively review the evidence for the prognostic usefulness of p53 immunohistochemical expression in paraffin-embedded tissue specimens from diffusely infiltrating astrocytomas. Paraffin 119-127 tumor protein p53 Homo sapiens 81-84 20459648-3 2010 The expressions of IDH1 and p53 in formalin-fixed paraffin-embedded tissue sections from 44 osteosarcoma patients were determined by immunohistochemistry, and the correlation between them and clinicopagthological features were analyzed. Paraffin 50-58 tumor protein p53 Homo sapiens 28-31 18780655-1 2008 AIM: To examine by means of immunohistochemistry the expression of the tumor suppressing gene p53 and gene p21 in cells of malignant melanoma of the uvea from formalin-paraffin material from patients, who were during the period 2000 - 2006 surgically treated due to malignant melanoma of the uvea at the Department of Ophthalmology in the University Hospital in Brno (Brunn), Czech Republic, E.U., and to correlate the results of the immunohistochemical detection with clinical signs of the tumor of each patient. Paraffin 168-176 tumor protein p53 Homo sapiens 94-97 19796081-0 2009 Oligonucleotide probe array for p53 gene alteration analysis in DNA from formalin-fixed paraffin-embedded breast cancer tissues. Paraffin 88-96 tumor protein p53 Homo sapiens 32-35 19796081-2 2009 We sequenced the p53 gene in DNA extracted from archival paraffin-embedded BC tissues and compared the results obtained from direct sequencing with those obtained by oligonucleotide probe array (OPA). Paraffin 57-65 tumor protein p53 Homo sapiens 17-20 19650989-5 2009 METHODS: p53 gene and chromosome 13q14 expression in paraffin sections of 30 cases of primary intestinal lymphoma and 10 cases of lymph node reactive hyperplasia were ascertained using an improved FISH technique. Paraffin 53-61 tumor protein p53 Homo sapiens 9-12 18619459-3 2008 METHODS: We immunohistochemically assayed p53 expression in paraffin-fixed colon cancer specimens in a large prospective cohort of women with 22 years of follow-up to examine the relationship of folate intake and intake of other one-carbon nutrients to risks by tumor p53 expression. Paraffin 60-68 tumor protein p53 Homo sapiens 42-45 18989634-3 2009 We defined P53 overexpression through immunohistochemistry of paraffin-embedded tumor tissues, using cutpoints based on percent of cells positive. Paraffin 62-70 tumor protein p53 Homo sapiens 11-14 19096907-6 2009 This study was designed to evaluate the efficacy of different fixatives, of microwaving and microwave pretreatment method to retrieve p53 immunoreactivity in paraffin-embedded non-lesioned (adjacent normal tissue) human skin samples or pathological human skin samples diagnosed as basal cell carcinoma. Paraffin 158-166 tumor protein p53 Homo sapiens 134-137 18330889-2 2008 We examined, for the first time, 196 paraffin-embedded CRC cases from Northern Iran for mutations in P53 exons 5-8 using PCR-direct sequencing. Paraffin 37-45 tumor protein p53 Homo sapiens 101-104 18348286-2 2008 We have developed a high-throughput scanning assay with automatic calling to detect TP53 mutations in DNA from fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissues. Paraffin 148-156 tumor protein p53 Homo sapiens 84-88 18393238-3 2008 Immunohistochemical staining for P53 was performed on paraffin-embedded sections. Paraffin 54-62 tumor protein p53 Homo sapiens 33-36 18221322-6 2008 LOH in paraffin-embedded specimens was detected in 22.6% and 12.9% for FHIT and p53 respectively. Paraffin 7-15 tumor protein p53 Homo sapiens 80-83 16835330-6 2006 p53 protein accumulation was assessed immunohistochemically using sections of paraffin-embedded benign breast tissue from 104 cases and 385 controls; for 82 of these cases and 327 of the controls, DNA was successfully extracted from the breast tissue for p53 gene analysis using PCR-single-strand conformation polymorphism/direct sequencing. Paraffin 78-86 tumor protein p53 Homo sapiens 0-3 18607961-5 2008 From these patients we chose 21 who had never smoked or drunk alcohol and performed immunohistochemical staining for p53 protein in paraffin-embedded archival blocks. Paraffin 132-140 tumor protein p53 Homo sapiens 117-120 17380529-3 2007 METHODS: P53 expression was determined by immunohistochemistry in paraffin-embedded tissue specimens from 107 consecutive patients (107 primary squamous cell carcinomas of the supraglottic larynx and 46 matched lymph node metastases). Paraffin 66-74 tumor protein p53 Homo sapiens 9-12 18507060-5 2008 Immunohistochemical reactions were performed for p53 staining in paraffin embedded histological sections of oral leukoplakia lesions. Paraffin 65-73 tumor protein p53 Homo sapiens 49-52 18048815-4 2007 MATERIALS AND METHODS: The complete coding region of the p53 gene was queried using DNA from paraffin-embedded tissues and employing a p53 gene-sequencing chip. Paraffin 93-101 tumor protein p53 Homo sapiens 57-60 16827107-5 2006 MATERIALS AND METHODS: Immunohistochemistry was employed to analyze the protein expression of p53, deltaNp73 and deltaNp63 in paraffin-embedded tumor samples from 132 well-characterized lung cancer patients. Paraffin 126-134 tumor protein p53 Homo sapiens 94-97 16569359-1 2006 Our study is aimed at evaluating the presence of p53 and Ki67 expression by immunohistochemistry in a series of 11 paraffin-embedded penile carcinomas. Paraffin 115-123 tumor protein p53 Homo sapiens 49-52 16700663-5 2006 An immunohistochemical method was used to investigate the expression of COX-2 and p53 proteins on formalin-fixed, paraffin-embedded tissue specimens of squamous cell carcinomas (SCC), basal cell carcinomas (BCC), Bowen"s disease (BD), actinic keratosis (AK) and porokeratosis. Paraffin 114-122 tumor protein p53 Homo sapiens 82-85 16649377-4 2006 Proper quality paraffin blocks were chosen for immunohistochemical staining for P53 through the immunoperoxidase method. Paraffin 15-23 tumor protein p53 Homo sapiens 80-83 16311126-6 2005 A p53 protein immunohistochemical analysis was performed on paraffin-embedded tissues. Paraffin 60-68 tumor protein p53 Homo sapiens 2-5 16311126-11 2005 Of 10 serous papillary carcinomas, 8 (80%) for which paraffin blocks were available overexpressed the p53 protein. Paraffin 53-61 tumor protein p53 Homo sapiens 102-105 16080576-3 2005 The IHC method of streptavidin biotin peroxidase on paraffin tissue sections was used to detect bcl2 and p53 oncoproteins and PAR4 pro-apoptotic protein expression in surgical specimens. Paraffin 52-60 tumor protein p53 Homo sapiens 105-108 16375215-4 2005 Immunostaining for p53 protein was performed on formalin-fixed, paraffin-embedded tissue sections, using the alkaline phosphatase antialkaline phosphatase (APAAP) method. Paraffin 64-72 tumor protein p53 Homo sapiens 19-22 15523893-6 2004 In this study, p53 protein immunoreactivity was investigated in paraffin sections of primary nasopharyngeal tumors and metastatic cervical lymph nodes and association with clinical and histopathological characteristics was evaluated. Paraffin 64-72 tumor protein p53 Homo sapiens 15-18 15459092-0 2004 Additional data for oligonucleotide arrays of the p53 gene in DNA from formalin-fixed, paraffin-embedded tissue. Paraffin 87-95 tumor protein p53 Homo sapiens 50-53 16158936-5 2005 Immunohistochemical staining for p53 was performed on paraffin-embedded tissue sections using the avidin-biotin-peroxidase method. Paraffin 54-62 tumor protein p53 Homo sapiens 33-36 15875732-0 2005 p53 alterations in sequential biopsies of Asian follicular lymphoma: a study of immunohistochemical staining pattern and gene mutations by PCR-SSCP in paraffin-embedded tissues. Paraffin 151-159 tumor protein p53 Homo sapiens 0-3 15875732-3 2005 We aimed to investigate the frequency of p53 gene alterations in paraffin-embedded tissue using commercially available PCR-SSCP, and to correlate the results with P53 protein expression by immunohistochemistry. Paraffin 65-73 tumor protein p53 Homo sapiens 41-44 15577317-2 2004 METHODS: The expression of p53 and Bcl-2 protein was assessed by immunohistochemistry from paraffin blocks. Paraffin 91-99 tumor protein p53 Homo sapiens 27-30 15603096-3 2004 p53 Protein was detected in paraffin sections using the monoclonal p53 antibody. Paraffin 28-36 tumor protein p53 Homo sapiens 0-3 15246568-0 2004 Analysis of p53 mutations in cells taken from paraffin-embedded tissue sections of ductal carcinoma in situ and atypical ductal hyperplasia of the breast. Paraffin 46-54 tumor protein p53 Homo sapiens 12-15 15363320-0 2004 [Application of immunohistochemistry-laser microdissection-PCR technique in detecting p53 gene mutation in paraffin sections of gastric cancer]. Paraffin 107-115 tumor protein p53 Homo sapiens 86-89 15363320-2 2004 METHODS: The expression of p53 protein in 41 paraffin-embedded advanced gastric cancer samples was assessed by immunohistochemistry. Paraffin 45-53 tumor protein p53 Homo sapiens 27-30 15363320-12 2004 CONCLUSIONS: IHC-LMD-PCR technique can be successfully applied in paraffin sections of gastric cancers for the detection of p53 gene mutations. Paraffin 66-74 tumor protein p53 Homo sapiens 124-127 15248926-2 2004 The purpose of this study was to evaluate the relationship of the cisplatin sensitivity/prognosis with the expression of excision repair cross complement-1 (ERCC-1), metallothionein (MT), and p53 in the paraffin-embedded tissue of advanced non-small cell lung cancer (NSCLC). Paraffin 203-211 tumor protein p53 Homo sapiens 192-195 15269478-6 2004 MATERIALS AND METHODS: p53 protein expression was evaluated on formalin-fixed paraffin-embedded sections using streptavidin biotin immunoperoxidase technique after high temperature antigen retrieval. Paraffin 78-86 tumor protein p53 Homo sapiens 23-26 15041112-4 2004 p53 immunostaining was performed on paraffin-embedded tissues using monoclonal antibody DO7 and an automated immunostainer. Paraffin 36-44 tumor protein p53 Homo sapiens 0-3 15099948-3 2004 Demonstration of p53 polymorphism was performed in DNA extracted from paraffin-embedded sections using the polymerase chain reaction (PCR). Paraffin 70-78 tumor protein p53 Homo sapiens 17-20 14726466-0 2004 Evaluation of oligonucleotide arrays for sequencing of the p53 gene in DNA from formalin-fixed, paraffin-embedded breast cancer specimens. Paraffin 96-104 tumor protein p53 Homo sapiens 59-62 15010889-4 2004 To further investigate the molecular mechanisms underlying bladder carcinogenesis with this specific etiology, mutation analysis of p53 gene (exon 5-8) was performed for 11 and 18 paraffin-embedded bladder cancers in Ukrainians, respectively collected before and after the Chernobyl disaster. Paraffin 180-188 tumor protein p53 Homo sapiens 132-135 14726466-2 2004 The purpose of this study was to determine whether a p53 microarray could be used to sequence the p53 gene in DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissues. Paraffin 145-153 tumor protein p53 Homo sapiens 98-101 14562283-6 2003 An immunohistochemical study of p53 expression was performed on paraffin-embedded tissue. Paraffin 64-72 tumor protein p53 Homo sapiens 32-35 12648880-4 2003 P53 expression was detected in paraffin-embedded endometrial specimens by immunohistochemical methods. Paraffin 31-39 tumor protein p53 Homo sapiens 0-3 12944180-1 2003 Aberrant p53 protein accumulation was measured immunohistologically in 342 colorectal paraffin-embedded tissue sections from 115 patients (24 with adenocarcinoma, 59 with adenoma and 32 "hospital controls"). Paraffin 86-94 tumor protein p53 Homo sapiens 9-12 12841678-7 2003 p53 protein accumulation was determined by immunohistochemistry on paraffin-embedded tumor specimens from 58/71 samples available for this study. Paraffin 67-75 tumor protein p53 Homo sapiens 0-3 12734658-4 2003 In the present study, we screened paraffin-embedded brain tissue from different areas of 18 cases (8 autopsy cases and 10 biopsies) for alterations in the TP53 and PTEN genes. Paraffin 34-42 tumor protein p53 Homo sapiens 155-159 12532420-5 2003 The mutation status of exons 5-8 of p53 was analyzed by denaturing high pressure liquid chromatography (DHPLC) in formalin-fixed, paraffin-embedded tumor sections, followed by direct sequencing of cases with aberrant chromatographic patterns. Paraffin 130-138 tumor protein p53 Homo sapiens 36-39 12824535-1 2003 This chapter describes the techniques necessary to identify p53 genomic mutations in either frozen or paraffin-embedded tumor samples. Paraffin 102-110 tumor protein p53 Homo sapiens 60-63 12559346-4 2003 METHODS: p16 and p53 expression were detected by immunohistochemical analysis of 90 paraffin specimens of resected NSCLC, including 35 squamous cell carcinoma, 47 adenocarcinoma, and eight large cell carcinoma, between stages I and IV. Paraffin 84-92 tumor protein p53 Homo sapiens 17-20 14995078-2 2003 This was a retrospective study in which MT and p53 immunopositive staining in 32 laryngeal SCC paraffin-embedded sections, were correlated with clinical recurrence. Paraffin 95-103 tumor protein p53 Homo sapiens 47-50 12493030-0 2003 p53 expression in normal paraffin-embedded tissue using different antibodies and antigen retrieval buffer systems. Paraffin 25-33 tumor protein p53 Homo sapiens 0-3 12493030-1 2003 AIMS: The study was undertaken to demonstrate wild-type p53 in normal paraffin-embedded tissues using two widely used antibodies, DO7 and 1801 and two different antigen retrieval buffer systems. Paraffin 70-78 tumor protein p53 Homo sapiens 56-59 12493030-10 2003 CONCLUSIONS: Our study demonstrates the presence of p53 in normal paraffin-embedded tissue with nuclear and/or cytoplasmic localization in some instances. Paraffin 66-74 tumor protein p53 Homo sapiens 52-55 12824536-4 2003 The most important is immunocytochemical p53 staining of sections from paraffin embedded tissues. Paraffin 71-79 tumor protein p53 Homo sapiens 41-44 12566814-4 2003 Immunohistochemical analysis for p53 was done on paraffin-fixed tissues with DO-7 antibodies. Paraffin 49-57 tumor protein p53 Homo sapiens 33-36 12759537-2 2003 The expression of the markers examined - Bcl-2, c-erbB-2, p53 - was detected in paraffin sections of the tumors by the streptavidin-biotin peroxidase method following heat-induced antigen retrieval, and the apoptosis rate was determined by the TUNEL method. Paraffin 80-88 tumor protein p53 Homo sapiens 58-61 12660452-5 2003 An immunohistochemical technique with monoclonal antibodies (DO-7) was used to detect the p53 phenotype in paraffin-fixed material. Paraffin 107-115 tumor protein p53 Homo sapiens 90-93 12167105-7 2002 Paraffin immunohistochemical analysis was carried out using anti-p53 and anti-Ki-67 antibodies on the initial tumor tissues. Paraffin 0-8 tumor protein p53 Homo sapiens 65-68 12192480-2 2002 MATERIAL AND METHODS: Immunohistochemical techniques were used to evaluate p53 expression in paraffin-embedded tissue specimens of 50 EOC cases. Paraffin 93-101 tumor protein p53 Homo sapiens 75-78 12552965-5 2002 Immunohistochemical analysis was performed to determine BRCA1 and the apoptosis-related proteins bcl-2, Bax and p53 in paraffin-embedded tissues of 156 sporadic invasive ductal carcinomas. Paraffin 119-127 tumor protein p53 Homo sapiens 112-115 12115529-5 2002 Immunoreactivity for maspin and p53 antibodies with paraffin-embedded carcinoma tissue was investigated using labeled streptavidin-biotin methods. Paraffin 52-60 tumor protein p53 Homo sapiens 32-35 12062599-5 2002 The status of p53 was immunohistochemically assessed in paraffin-embedded pretreatment biopsy specimens, along with appropriate controls. Paraffin 56-64 tumor protein p53 Homo sapiens 14-17 12451999-2 2002 METHODS: Seventy-nine patients with cervical cancer and 23 with cases of normal cervical epithelium in geriatric people were investigated immunohistochemically for expression of p53 and c-erbB2 in paraffin sections. Paraffin 197-205 tumor protein p53 Homo sapiens 178-181 11927338-3 2002 METHODS: The expression of p53, mdm2, and bcl-2 protein was studied by immunohistochemical methods in paraffin-embedded nephrectomy specimens from 112 patients whose clinicopathologic data confirmed renal cell carcinoma. Paraffin 102-110 tumor protein p53 Homo sapiens 27-30 11914633-10 2002 Examination of the microdissected paraffin-embedded tissues revealed p53 mutations in the malignant epithelioid areas in AML but not in the renal parenchyma or typical AML areas. Paraffin 34-42 tumor protein p53 Homo sapiens 69-72 12065876-2 2002 METHODS: The expression of p53 and mdm2 was analyzed by an immunohistochemical assay in 80 formalin-fixed paraffin embedded primary tumour samples and related to the clinical response to 5-fluorouracil therapy and to the prognosis of the patients. Paraffin 106-114 tumor protein p53 Homo sapiens 27-30 12002339-2 2002 p53 immunohistochemical expression was determined in archival paraffin embedded tissue blocks of 30 breast sarcoma patients, (19 fibrosarcomas, nine malignant fibrohistiocytomas and two liposarcomas) treated at the Hospital do Cancer AC Camargo, Sao Paulo, Brazil from 1955 to 1990. Paraffin 62-70 tumor protein p53 Homo sapiens 0-3 12056646-5 2002 A series of 129 oral squamous cell cancers was investgated retrospectively for expression of P53 protein by immunohistochemistry of paraffin-embedded tissue sections. Paraffin 132-140 tumor protein p53 Homo sapiens 93-96 11449307-2 2001 The purpose of the present study was to analyze the expression of the oncogenes c-erbB-2 and c-myc and of the suppressor gene p53 by immunohistochemical techniques in archival paraffin-embedded tissue blocks of 48 male breast cancer patients, treated at the A.C. Camargo Cancer Hospital, Sao Paulo, SP, Brazil. Paraffin 176-184 tumor protein p53 Homo sapiens 126-129 11740051-3 2001 Immunohistochemical staining method was used for detecting p53 and bcl-2 expression on paraffin blocks of three pure insular, five follicular or papillary thyroid carcinomas with a major insular component (more than 50%) and six with a minor insular component (20-50%). Paraffin 87-95 tumor protein p53 Homo sapiens 59-62 11445851-3 2001 To characterize the involvement of the p53 gene abnormality in this disease, we analyzed expression and sequence alteration of p53 by immunohistochemical analysis of the protein expression and quantitative DNA/PCR and PCR-SSCP assays of the gene in 28 paraffin-embedded tissue specimens. Paraffin 252-260 tumor protein p53 Homo sapiens 127-130 11289281-4 2001 METHODS: Mutation screening of the hot spots of the K-ras gene and of the evolutionarily conserved regions of the TP53 gene was performed by denaturing gradient gel electrophoresis technique in formalin-fixed paraffin-embedded specimens of 55 consecutive patients with Dukes C colon cancer treated with adjuvant 5-fluorouracil-based chemotherapy. Paraffin 209-217 tumor protein p53 Homo sapiens 114-118 11358811-3 2001 We identified mutations in exons 5-8 of p53 using DNA extracted from formalin-fixed paraffin-embedded tissue blocks from 146 whites and 26 non-whites with astrocytic glioma by PCR-single-strand conformation polymorphism and direct sequencing. Paraffin 84-92 tumor protein p53 Homo sapiens 40-43 11316543-4 2001 METHODS AND MATERIALS: The nuclear expression of HAP1 and p53 proteins was studied by immunohistochemistry in paraffin-embedded material from 95 patients with locally advanced squamous cell head-and-neck cancer (HNC) treated with radical radiotherapy (38 cases with induction platinum-based chemotherapy and 57 with concurrent platinum chemoradiotherapy). Paraffin 110-118 tumor protein p53 Homo sapiens 58-61 11692147-2 2001 Immunohistochemical analysis of MDM2 and p53 proteins on paraffin embedded sections from 64 surgically resected ESCCs and matched histologically normal tissues showed overexpression of MDM2 protein in 23/64 (36%) ESCCs, while the histopathologically normal esophageal tissues did not show detectable level of MDM2 immunoreactivity. Paraffin 57-65 tumor protein p53 Homo sapiens 41-44 11051374-4 2000 Mutation detection of the KRAS and TP53 genes was performed on paraffin-embedded tumor material, using denaturating gradient gel electrophoresis. Paraffin 63-71 tumor protein p53 Homo sapiens 35-39 11148457-1 2001 Expression of p53 protein and MDM2 was evaluated in paraffin-embedded tissue from 78 patients with papillary carcinomas of the thyroid (PCT), in order to elucidate the relationship between them and their correlations with some clinicopathologic features implicated in tumor progression. Paraffin 52-60 tumor protein p53 Homo sapiens 14-17 10780666-3 2000 Expression and mutations of the p53 gene were examined in the paraffin-embedded specimens of the nasal lesions from 42 Chinese (Beijing and Chengdu) and Japanese (Okinawa and Osaka) patients with nasal NK/T-cell lymphoma by the immunohistochemistry and single strand conformation polymorphism (SSCP) analysis of polymerase chain reaction (PCR) amplified products followed by direct sequencing. Paraffin 62-70 tumor protein p53 Homo sapiens 32-35 11268475-3 2000 PATIENTS AND METHODS: Nuclear immunohistochemical expression of p53 protein was determined in formalin-fixed paraffin-embedded archival tumor tissue samples from 190 primary colorectal adenocarcinomas. Paraffin 109-117 tumor protein p53 Homo sapiens 64-67 10953132-2 2000 MATERIALS AND METHODS: Immunohistochemical staining for p53 was semiquantitatively scored in archival formalin fixed, paraffin embedded tumor tissue obtained at diagnosis in 221 patients with prostate cancer. Paraffin 118-126 tumor protein p53 Homo sapiens 56-59 10948336-3 2000 To characterize the involvement of the p53 gene abnormality in this disease, we analyzed expression and sequence alteration of p53 by immunohistochemical analysis of the protein expression and quantitative DNA/PCR and PCR-SSCP assays of the gene in 33 paraffin-embedded tissue specimens. Paraffin 252-260 tumor protein p53 Homo sapiens 127-130 10948341-5 2000 Immunostaining for p53 protein with 4 different antibodies was performed on paraffin-embedded specimens from 69 patients with adenocarcinoma and squamous cell carcinoma of the oesophagus. Paraffin 76-84 tumor protein p53 Homo sapiens 19-22 10897337-3 2000 This is a highly selective approach, whereby immunostained sections of formalin fixed, paraffin wax embedded tissue are exposed to ultraviolet irradiation to damage the DNA in p53 negative cells. Paraffin 87-99 tumor protein p53 Homo sapiens 176-179 11127510-0 2000 Mutation analysis of the p53 tumor suppressor gene using paraffin-embedded specimens of human transitional cell carcinomas by the direct sequencing method. Paraffin 57-65 tumor protein p53 Homo sapiens 25-28 10815906-4 2000 The overexpression of mdm2 and p53 proteins was investigated on paraffin-embedded material by using monoclonal antibodies. Paraffin 64-72 tumor protein p53 Homo sapiens 31-34 10809364-4 2000 28 gastric dysplasia, 45 Early GC and 98 Advanced Gastric Cancers were evaluated for expression of the oncogenes p53, c-ErbB2, c-myc and the EGFr in paraffin-embedded material utilizing Avidin-Biotin immunohistochemistry techniques. Paraffin 149-157 tumor protein p53 Homo sapiens 113-116 10850547-2 2000 A novel polymerase chain reaction (PCR) approach for the analysis of the entire p53 coding and splice site regions from microdissected, formalin-fixed, paraffin-embedded tumor tissues has been developed which allows multiple genetic analyses to be performed from one primary amplification reaction. Paraffin 152-160 tumor protein p53 Homo sapiens 80-83 10874665-6 2000 Mdm2 and p53 were demonstrated by immunohistology on formalin-fixed and paraffin-embedded tumor tissue. Paraffin 72-80 tumor protein p53 Homo sapiens 9-12 10885605-5 2000 p53 staining was performed by immunohistochemistry, using the monoclonal antibody DO7 on paraffin-embedded tissue. Paraffin 89-97 tumor protein p53 Homo sapiens 0-3 10636514-5 2000 Immunohistochemical staining was performed to assess p53 expression in paraffin-embedded ovarian cancers. Paraffin 71-79 tumor protein p53 Homo sapiens 53-56 10751531-3 2000 Silver staining PCR-SSCP method was used to detect mutations in exons 5, 6, 7, 8 of p53 gene and MSI at 4 loci on chromosomes 2, 5, 17 in the 73 paraffin-embedded biopsy specimens, and the relationship between them was studied further. Paraffin 145-153 tumor protein p53 Homo sapiens 84-87 10927863-3 2000 Paraffin sections were stained by using monoclonal antibody D07 for p53 protein and PC-10 for PCNA. Paraffin 0-8 tumor protein p53 Homo sapiens 68-71 10718209-7 2000 In the present study the authors optimized a polymerase chain reaction-based mutation screening method, fluorescence-single strand conformation polymorphism (F-SSCP), that allows p53 status to be assessed accurately and reproducibly in routinely handled, formalin-fixed and paraffin-embedded tumor specimens. Paraffin 274-282 tumor protein p53 Homo sapiens 179-182 10682666-3 2000 Immunohistochemical p53 (pAb 1801 mAb) and TS (TS106 mAb) expression on formalin-fixed paraffin-embedded primary tumour specimens was related to probability of clinical response to chemotherapy, time to progression and overall survival. Paraffin 87-95 tumor protein p53 Homo sapiens 20-23 10618603-4 2000 Immunohistochemical expression of nuclear p53 protein was assessed in formalin fixed, paraffin embedded archival tumor tissue. Paraffin 86-94 tumor protein p53 Homo sapiens 42-45 10626170-4 1999 METHODOLOGY: In a retrospective case-series p53 expression was assessed using standard immunohistochemical methods in the paraffin-embedded specimens of 45 liver resections for colorectal metastases, performed in 43 patients in a single institution between "86 and "96. Paraffin 122-130 tumor protein p53 Homo sapiens 44-47 10974929-4 2000 p53 protein accumulation was detected by immunohistochemistry using antibodies against p53: NCL-p53 (clone BP-53-12) (Novocastra) on paraffin embedded specimens. Paraffin 133-141 tumor protein p53 Homo sapiens 0-3 10628802-1 1999 TP53 and MDM2 genes and their protein expression were evaluated in frozen and paraffin-embedded tissue from 27 patients with malignant fibrous histiocytoma to elucidate the relationship between them, their implication in tumor progression mechanisms and their possible diagnostic-prognostic value in malignant fibrous histiocytoma. Paraffin 78-86 tumor protein p53 Homo sapiens 0-4 10606817-6 1999 Sequence analysis of exons 5 through 8 of p53 was performed on cloned PCR-amplified DNA of formalin-fixed, paraffin-embedded tumors. Paraffin 107-115 tumor protein p53 Homo sapiens 42-45 10632350-1 1999 To evaluate both the clinicopathological and prognostic significance of p53 protein, the expression of p53 protein was immunohistochemically examined by use of CM1, PAb1801, DO7, and DO1 antibodies on paraffin-embedded colorectal adenocarcinomas from 293 patients. Paraffin 201-209 tumor protein p53 Homo sapiens 103-106 10349985-4 1999 bcl-2 and p53 protein expression were demonstrated by immunohistochemical methods, using formalin-fixed, paraffin-embedded biopsy tissues. Paraffin 105-113 tumor protein p53 Homo sapiens 10-13 10427139-5 1999 Sequence analysis of exons 5 through 8 of p53 was performed on subcloned PCR-amplified DNA, extracted from formalin-fixed, paraffin-embedded tumors. Paraffin 123-131 tumor protein p53 Homo sapiens 42-45 10459542-6 1999 P53 mutations were analyzed on DNA extracted from paraffin-embedded specimens with use of single strand conformation polymorphism, followed by direct sequencing. Paraffin 50-58 tumor protein p53 Homo sapiens 0-3 10629648-5 1999 Mutant p53 protein IHC overexpression was examined from paraffin embedded tissues using monoclonal DO-7 Ab. Paraffin 56-64 tumor protein p53 Homo sapiens 7-10 10483067-2 1999 MATERIALS AND METHODS: The expression of p53 and MDM2 proteins was determined immunohistochemically in 51 formalin-fixed, paraffin embedded specimens of odontogenic cysts and tumours. Paraffin 122-130 tumor protein p53 Homo sapiens 41-44 10326590-6 1999 We examined TP53 protein expression from paraffin-embedded colon tissue of the patient with an immunohistochemical method. Paraffin 41-49 tumor protein p53 Homo sapiens 12-16 10518106-5 1999 METHODS: p53 expression in formalin-fixed, paraffin-embedded samples of 239 patients with primary esophageal squamous cell carcinoma (TNM stage I:79 cases, stage II: 82 cases, stage III: 78 cases), who underwent esophageal resection without additional treatment, were analyzed by immunohistochemical staining using a polyclonal antibody, RSP53. Paraffin 43-51 tumor protein p53 Homo sapiens 9-12 10621843-3 1999 p53 protein expression was evaluated by immunohistochemical analysis on paraffin-embedded sections of 62 representative oral cancer biopsies and 22 leukoplakias, using p53-specific monoclonal antibody DO-7. Paraffin 72-80 tumor protein p53 Homo sapiens 0-3 9886570-3 1998 Exons 5-8 of the p53 gene were examined for mutations by the polymerase chain reaction-single strand conformation polymorphism technique and DNA sequencing, using DNA from formalin-fixed paraffin-embedded tissues. Paraffin 187-195 tumor protein p53 Homo sapiens 17-20 10690631-3 1999 The positive clones selected by ELISA were found to exhibit strong staining of nuclear p53 in both fresh and archival paraffin embedded breast tumour tissue sections. Paraffin 118-126 tumor protein p53 Homo sapiens 87-90 12938516-1 1999 To understand the relationship between expression of P53 protein and HPV16/18 infection in laryngeal papillomas, PCR and immunohistochemical techniques were used to examine the paraffin-embedded tissue samples of laryngeal papillomas from 35 subjects. Paraffin 177-185 tumor protein p53 Homo sapiens 53-56 10408787-0 1999 Comprehensive TP53-denaturing gradient gel electrophoresis mutation detection assay also applicable to archival paraffin-embedded tissue. Paraffin 112-120 tumor protein p53 Homo sapiens 14-18 10408787-4 1999 When testing the assay on DNA from cultured lung cancer cell lines and from paraffin-embedded Dukes C colorectal carcinomas, significant TP53 mutations were observed at high frequencies in 15 of 16 lung cancer cell lines (94%) and in 21 of 30 paraffin-embedded tissue samples of Dukes C colorectal carcinomas (70%). Paraffin 76-84 tumor protein p53 Homo sapiens 137-141 10408787-4 1999 When testing the assay on DNA from cultured lung cancer cell lines and from paraffin-embedded Dukes C colorectal carcinomas, significant TP53 mutations were observed at high frequencies in 15 of 16 lung cancer cell lines (94%) and in 21 of 30 paraffin-embedded tissue samples of Dukes C colorectal carcinomas (70%). Paraffin 243-251 tumor protein p53 Homo sapiens 137-141 10071687-3 1999 Immunostaining for p53 protein was performed on paraffin and frozen sections from 61 patients with different grades of meningiomas using monoclonal antibodies (mAbs) DO-1 and pAb240. Paraffin 48-56 tumor protein p53 Homo sapiens 19-22 9655287-4 1998 Histopathologic/genetic analysis of p53 was performed on formalin fixed, paraffin embedded tissues. Paraffin 73-81 tumor protein p53 Homo sapiens 36-39 9784320-3 1998 p53 expression was studied in archival paraffin-embedded tissue by immunohistochemistry. Paraffin 39-47 tumor protein p53 Homo sapiens 0-3 9579543-3 1998 p53 protein immunohistochemistry was performed on 37 paraffin embedded biopsies from 27 patients with CTCL; LCT was present in 15 biopsies. Paraffin 53-61 tumor protein p53 Homo sapiens 0-3 9698472-2 1998 Immunostaining for p53 protein was performed on formalin-fixed, paraffin-embedded sections from 158 patients with verified uterine sarcomas using monoclonal p53 antibody (DO-1). Paraffin 64-72 tumor protein p53 Homo sapiens 19-22 9645763-3 1998 In the present study, p53 gene mutations were analyzed on paraffin-embedded specimens from 24 patients with PRS (4 men and 20 women) by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) followed by direct sequencing. Paraffin 58-66 tumor protein p53 Homo sapiens 22-25 9595036-8 1998 Strong positive nuclear staining of the p53 protein was detected in both normal and tumor paraffin-embedded tissues. Paraffin 90-98 tumor protein p53 Homo sapiens 40-43 9797697-4 1998 Immunohistochemical expression of p53 protein was evaluated in formalin-fixed paraffin-embedded sections of CRC liver metastases using the monoclonal antibodies (MAbs) D01 and Pab 1801. Paraffin 78-86 tumor protein p53 Homo sapiens 34-37 9529014-5 1998 Immunohistochemical staining of the paraffin sections was performed using monoclonal antibodies to cyclin E and p53. Paraffin 36-44 tumor protein p53 Homo sapiens 112-115 9607618-9 1998 p53 mutant status was assessed immunohistochemically from sections of the formalin-fixed, paraffin-embedded pretreatment biopsy and the resected specimen. Paraffin 90-98 tumor protein p53 Homo sapiens 0-3 9445187-12 1998 CONCLUSIONS: In this study, p53p overexpression was infrequent in paraffin embedded melanoma specimens and independent of the primary melanoma"s anatomic site. Paraffin 66-74 tumor protein p53 Homo sapiens 28-32 9510045-3 1998 MATERIALS AND METHODS: Immunostaining for p53 protein was performed on paraffin-embedded specimens from 69 patients with adeno- and squamous cell carcinoma of the oesophagus. Paraffin 71-79 tumor protein p53 Homo sapiens 42-45 9609668-0 1998 Simultaneous detection of p53 nuclear protein and chromosome aberrations on sections from formalin-fixed, paraffin-embedded breast cancer tissue. Paraffin 106-114 tumor protein p53 Homo sapiens 26-29 9515788-3 1998 We analyzed p53 mutations on paraffin-embedded specimens from 21 patients with PAL by PCR-single-strand conformational polymorphism followed by direct sequencing. Paraffin 29-37 tumor protein p53 Homo sapiens 12-15 9682769-6 1998 p53 protein expression was evaluated by immunohistochemistry (IHC) on paraffin-embedded sections. Paraffin 70-78 tumor protein p53 Homo sapiens 0-3 9719502-4 1998 In addition, p53 protein overexpression was assessed by immunohistochemistry using the monoclonal antibody DO-7 on paraffin-embedded tissue sections. Paraffin 115-123 tumor protein p53 Homo sapiens 13-16 9580543-4 1998 Expression of mutant p53 was evaluated in 655 human breast carcinomas (349 node-positive and 306 node-negative patients) with long-term clinical follow-up by immunohistochemistry in sections from paraffin embedded tumors. Paraffin 196-204 tumor protein p53 Homo sapiens 21-24 9773296-1 1998 Immunohistochemical detection of p53 in paraffin-embedded biopsy specimens has important diagnostic, prognostic and therapeutic applications. Paraffin 40-48 tumor protein p53 Homo sapiens 33-36 15678851-4 1998 In this study we analyzed the expression of p53 protein in a variety of salivary gland malignant tumors fixed in formalin and included in paraffin, using the method of immunohistochemical coloring with the anti-p53 DO-7 antibody. Paraffin 138-146 tumor protein p53 Homo sapiens 44-47 15678854-6 1998 Immunohistochemical determination of p53 protein was performed on tissular samples formalin fixed and paraffin embedded, using mouse monoclonal antibody DAKO--p53 DO 7 p53 human antiprotein. Paraffin 102-110 tumor protein p53 Homo sapiens 37-40 10374328-2 1997 METHODS: The expression of p53 protein was determined in the paraffin embedded tissues of 171 cases of cervical carcinoma, 68 cases of SIL and 29 cases of chronic cervicitis, using ABC immunohistochemical method. Paraffin 61-69 tumor protein p53 Homo sapiens 27-30 9416838-4 1997 In this study we addressed the possible prognostic value of the molecular alterations identified in exons 5-8 of the TP53 gene in DNAs from 151 paraffin-embedded NSCLC sections corresponding to 59 Spanish and 92 Polish stage I-IIIA resected patients. Paraffin 144-152 tumor protein p53 Homo sapiens 117-121 9214672-7 1997 Mutation of the p53 gene was indicated by overexpression of p53 protein (i.e., cellular accumulation of mutant p53 protein) in paraffin-embedded ovarian cancer tissue blocks; the mutant protein was detected by means of standard immunohistochemical techniques. Paraffin 127-135 tumor protein p53 Homo sapiens 16-19 9494587-2 1997 We investigated the localization of p53 protein in association with HPV in paraffin sections of 10 dysplastic and 12 malignant laryngeal squamous epithelium specimens by using immunohistochemical and in situ hybridization techniques. Paraffin 75-83 tumor protein p53 Homo sapiens 36-39 9368590-1 1997 We investigated the expression of p53 in 82 formalin-fixed, paraffin-embedded archival tissue specimens of lip and intraoral squamous cell carcinomas (SCCs) from the period 1930-1995, by immunohistochemistry using three monoclonal antibodies (MAbs DO-7, DO-1 and 1801). Paraffin 60-68 tumor protein p53 Homo sapiens 34-37 10920912-2 1997 METHODS: Levels of c-erbB-2 and p53 protein were detected by immunohistochemistry in 184 paraffin-embeded blocks. Paraffin 89-97 tumor protein p53 Homo sapiens 32-35 9397628-4 1997 Paraffin-embedded tissue blocks of surgical margins from 24 patients with oral cavity and oropharyngeal squamous cell carcinoma were immunostained for p53 protein. Paraffin 0-8 tumor protein p53 Homo sapiens 151-154 9290950-4 1997 The expression of p53 was determined by immunohistochemistry in 19 pheochromocytomas and in 17 MTCs using two antibodies (D01 and D07) on frozen and paraffin-embedded tissues. Paraffin 149-157 tumor protein p53 Homo sapiens 18-21 10325638-1 1997 To explore the carcinogenic effect of asbestos at molecular level, we directly analyzed the mutations of exon 5, 7 and 8 of anti-oncogene p53 in paraffin-embedded human lung tissue affected by asbestos for the first time. Paraffin 145-153 tumor protein p53 Homo sapiens 138-141 9214672-7 1997 Mutation of the p53 gene was indicated by overexpression of p53 protein (i.e., cellular accumulation of mutant p53 protein) in paraffin-embedded ovarian cancer tissue blocks; the mutant protein was detected by means of standard immunohistochemical techniques. Paraffin 127-135 tumor protein p53 Homo sapiens 60-63 9214672-7 1997 Mutation of the p53 gene was indicated by overexpression of p53 protein (i.e., cellular accumulation of mutant p53 protein) in paraffin-embedded ovarian cancer tissue blocks; the mutant protein was detected by means of standard immunohistochemical techniques. Paraffin 127-135 tumor protein p53 Homo sapiens 60-63 9301456-1 1997 This short report describes the detection of mutations of the TP53 tumour suppressor gene in sporadic ovarian carcinomas using archival paraffin-embedded tissues and automated fluorescent DNA sequencing. Paraffin 136-144 tumor protein p53 Homo sapiens 62-66 9812577-0 1997 [Detection of p53 gene mutation in paraffin-embedded asbestos-related lung cancer tissue]. Paraffin 35-43 tumor protein p53 Homo sapiens 14-17 9216855-4 1997 To assess the relationship between the LOH status of the p53 gene in the liver cirrhosis stage and that in HCC, we analyzed the samples microdissected from paraffin-embedded tissues using the polymerase-chain-reaction-based assay. Paraffin 156-164 tumor protein p53 Homo sapiens 57-60 9236923-7 1997 METHODS: Expression of p53 and PCNA were detected by immunocytochemistry in paraffin-embedded sections of infiltrating duct carcinoma and control breast tissue (normal tissue and adenoma). Paraffin 76-84 tumor protein p53 Homo sapiens 23-26 9179065-7 1997 p53 immunostaining was performed on paraffin embedded tissue. Paraffin 36-44 tumor protein p53 Homo sapiens 0-3 9191518-5 1997 METHODS: The authors analyzed the numeric aberrations of chromosome 17 and p53 gene deletions in 11 paraffin embedded pleomorphic adenomas (PA) and 9 carcinomas in pleomorphic adenoma (CIPA), using FISH techniques. Paraffin 100-108 tumor protein p53 Homo sapiens 75-78 9185695-3 1997 We analyzed p53 mutations on paraffin-embedded specimens from 33 patients with AS by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) followed by direct sequencing. Paraffin 29-37 tumor protein p53 Homo sapiens 12-15 9205870-0 1997 Detectability of retinoblastoma (Rb) gene product and p53 antigen expression decreases in stored paraffin sections. Paraffin 97-105 tumor protein p53 Homo sapiens 54-57 9129144-5 1997 Positive staining of p53 protein was observed in three of the paraffin embedded tissues that were available: brain tumor, rhabdomyosarcoma, and lymphocytes from a normal lymph node from the rhabdomyosarcoma patient. Paraffin 62-70 tumor protein p53 Homo sapiens 21-24 9136508-1 1997 Immunohistochemical analysis of the p53 protein was performed on paraffin sections of tumor tissue from 210 breast cancer patients. Paraffin 65-73 tumor protein p53 Homo sapiens 36-39 12114668-5 1997 Immunohistochemical staining for p53 was performed on paraffin sections of formalin-fixed tumor tissue. Paraffin 54-62 tumor protein p53 Homo sapiens 33-36 9076236-3 1997 DESIGN: We screened for p53 protein expression in a variety of benign epithelial lesions of upper respiratory tract using monoclonal antibody DO-1 on paraffin-embedded material. Paraffin 150-158 tumor protein p53 Homo sapiens 24-27 9215149-1 1997 AIM: To document the frequency and extent of p53 gene product expression in paraffin sections of resected non-small cell carcinoma of the lung and in cytological preparations of the same tumours; to determine the effect of microwave antigen retrieval on antigen detection. Paraffin 76-84 tumor protein p53 Homo sapiens 45-48 9075790-1 1997 The relationship between p53 overexpression and clinicopathologic variables in gastric cancer was evaluated using 304 paraffin-embedded gastric tumor tissues. Paraffin 118-126 tumor protein p53 Homo sapiens 25-28 9071734-0 1997 A rapid and nonisotopic method for the screening and sequencing of p53 gene mutations in formalin-fixed, paraffin-embedded tumors. Paraffin 105-113 tumor protein p53 Homo sapiens 67-70 9071734-2 1997 In this study, we describe a sensitive, rapid, nonisotopic and inexpensive procedure for the polymerase chain reaction (PCR)-single-stranded conformational polymorphism (SSCP) detection and subsequent sequencing of p53 mutations in formalin-fixed and paraffin-embedded tumor (PET) samples. Paraffin 251-259 tumor protein p53 Homo sapiens 215-218 9260591-4 1997 The expression of the proteins p53 and mdm2 was determined immunohistochemically using formalin-fixed, paraffin-embedded material. Paraffin 103-111 tumor protein p53 Homo sapiens 31-34 9058631-4 1997 METHODS: The p53 expression in tumour tissue was studied by immunohistochemistry using CM1 polyclonal rabbit antibody and formalin-fixed, paraffin-embedded material. Paraffin 138-146 tumor protein p53 Homo sapiens 13-16 8980360-3 1997 Genomic p53 was amplified with the polymerase chain reaction (PCR) from formalin-fixed, paraffin-embedded tissues. Paraffin 88-96 tumor protein p53 Homo sapiens 8-11 9032539-2 1997 MATERIALS AND METHODS: The p53 protein in the paraffin-embedded materials taken from 72 patients with RCCs was evaluated immunohistochemically and was compared with the histological findings, expression of proliferating cell nuclear antigen (PCNA), genetic instability as assessed by 2c deviation index (2cDI) and 5c exceeding rate (5cER) as well as clinical outcome. Paraffin 46-54 tumor protein p53 Homo sapiens 27-30 9393591-3 1997 p53 nuclear accumulation was detected in 48% of the primary tumours using the PAb1801 antibody in archival paraffin-embedded tissue sections. Paraffin 107-115 tumor protein p53 Homo sapiens 0-3 9313826-2 1997 The p53 immunohistochemistry was performed on paraffin sections from 88 tumour samples. Paraffin 46-54 tumor protein p53 Homo sapiens 4-7 8995554-6 1997 We conclude that PCR-TGGE is an appropriate method for detection of p53 point mutations in paraffin-embedded material. Paraffin 91-99 tumor protein p53 Homo sapiens 68-71 9868092-1 1997 Using polymerase chain reaction single strand conformational polymorphism (PCR-SSCP) and EB staining technique, paraffin-embeded sections of 20 hydatidiform mole and 4 choriocarcinoma were detected in the exons 5 and 8 of p53 gene. Paraffin 112-120 tumor protein p53 Homo sapiens 222-225 10073037-0 1997 [Study of detecting p53 gene of laryngeal carcinoma on paraffin-embedded tissue sections by in situ polymerase chain reaction]. Paraffin 55-63 tumor protein p53 Homo sapiens 20-23 10073037-1 1997 In order to investigate alterations of p53 gene in laryngeal carcinoma, five cases of paraffin-embedded tissue were detected by in situ polymerase chain reaction (PCR). Paraffin 86-94 tumor protein p53 Homo sapiens 39-42 9342752-6 1997 Our results indicate that IHC could be applied for studies of p53 protein accumulation in archival formalin fixed, paraffin-embedded bladder tumours. Paraffin 115-123 tumor protein p53 Homo sapiens 62-65 8761369-0 1996 TP53 mutation analyses on breast carcinomas: a study of paraffin-embedded archival material. Paraffin 56-64 tumor protein p53 Homo sapiens 0-4 9521509-1 1997 The immunohistological expression of p53 and MDM2 oncoproteins was examined in paraffin embedded tissue from 106 patients with transitional cell carcinoma of the urinary bladder and was related to various clinicopathological features, the expression of proliferation associated markers (proliferating cell nuclear antigen - PCNA - and Ki-67), c-erb B-2 oncoprotein and epidermal growth factor receptor (EGFR), as well as to survival. Paraffin 79-87 tumor protein p53 Homo sapiens 37-40 8955620-0 1996 Detection of p53 gene abnormality by sequence analysis of archival paraffin tissue. Paraffin 67-75 tumor protein p53 Homo sapiens 13-16 8942496-3 1996 STUDY DESIGN: Immunohistochemical evaluation was used to examine p53 expression in paraffin blocks from 179 endometrial adenocarcinomas. Paraffin 83-91 tumor protein p53 Homo sapiens 65-68 8942499-3 1996 Overexpression of p53 protein was determined with the use of immunohistochemistry on fixed and paraffin-embedded tissue. Paraffin 95-103 tumor protein p53 Homo sapiens 18-21 8808919-3 1996 MATERIALS AND METHODS: The expression of p53 and bcl-2 was evaluated in archival paraffin-embedded RP specimens from 175 patients followed from 1 to 9 years (mean = 4.6 years) and correlated with stage, grade, race and serologic (PSA) recurrence following surgery. Paraffin 81-89 tumor protein p53 Homo sapiens 41-44 8683636-1 1996 BACKGROUND: We previously observed decreased p53 immunostaining over time in paraffin-embedded sections of ductal carcinoma in situ of the breast of women; these sections had been stored on slides at room temperature. Paraffin 77-85 tumor protein p53 Homo sapiens 45-48 8879300-2 1996 The accumulation of p53 protein was studied immunohistochemically using monoclonal antibody BP53-12 on formalin-fixed paraffin-embedded sections of 36 KS lesions, of which 14 were classified histologically as early type and 22 as spindle-cell or mixed type. Paraffin 118-126 tumor protein p53 Homo sapiens 20-23 9387269-1 1996 Paraffin-embedded hepatocellular carcinoma (HCC) samples were used to study point mutation of p53 gene at 249 codon in the exon 7 by polymerase chain reaction and gel electrophoresis under Hae III enzyme digestion. Paraffin 0-8 tumor protein p53 Homo sapiens 94-97 8761369-1 1996 The aim of this investigation was to examine the possibility of analysing TP53 mutations in archival paraffin-embedded material with the constant denaturant gel electrophoresis (CDGE) method. Paraffin 101-109 tumor protein p53 Homo sapiens 74-78 8797205-1 1996 Evaluation of the p53 gene protein expression and proliferative potential with MIB-1 monoclonal antibody (MBL Co.), a new marker of cellular proliferation that binds Ki67 in paraffin sections, by immunohistochemical studies were made in 10 cases of the non-recurrent meningiomas and the 8 cases (17 samples) of the recurrent meningiomas. Paraffin 174-182 tumor protein p53 Homo sapiens 18-21 8881915-2 1996 METHODS: Expression of p53 was evaluated immunocytochemically in a retrospective study of formalin fixed, paraffin wax embedded tissue. Paraffin 106-118 tumor protein p53 Homo sapiens 23-26 8818693-2 1996 To investigate whether overexpressed bcl-2 and abnormally stabilized p53 are associated with reduced apoptosis in paraffin sections of non-small cell lung carcinoma, apoptotic, mitotic, and Ki-67 labelling indices were determined and correlated with bcl-2 and p53 immunoreactivity in 54 squamous cell carcinomas and 22 adenocarcinomas. Paraffin 114-122 tumor protein p53 Homo sapiens 69-72 8912347-3 1996 In this study we examined both benign and borderline malignant/malignant mucinous neoplasms for p53 protein accumulation by the means of an anti-human p53 protein monoclonal antibody on paraffin sections. Paraffin 186-194 tumor protein p53 Homo sapiens 96-99 8663952-2 1996 OBJECTIVE: To demonstrate how the detection of p53 protein in formaldehyde-fixed, paraffin-embedded oropharyngeal carcinoma may be used as a factor in estimating prognosis. Paraffin 82-90 tumor protein p53 Homo sapiens 47-50 8816839-4 1996 Protein p53 expression was evaluated by immunohistochemistry using Pab 1801, Pab 240, DO7 and CM1 antibodies on paraffin-embedded sections. Paraffin 112-120 tumor protein p53 Homo sapiens 8-11 12114639-0 1996 Possible Relation of p53 and mdm-2 Oncoprotein Expression in Thyroid Carcinoma: A Molecular-Pathological and Immunohistochemical Study on Paraffin-Embedded Tissue. Paraffin 138-146 tumor protein p53 Homo sapiens 21-24 8782209-10 1996 We used the DO-7 monoclonal antibody reactive for the N-terminal of the p53 protein on formalin-fixed paraffin-embedded tissue. Paraffin 102-110 tumor protein p53 Homo sapiens 72-75 8683246-3 1996 bcl2 and p53 protein expression was studied on paraffin-embedded tumor tissue by immunohistochemistry in relation to clinical factors. Paraffin 47-55 tumor protein p53 Homo sapiens 9-12 16696068-0 1996 Nested PCR-SSCP assay for the detection of p53 mutations in paraffin wax embedded bone tumours: improvement of sensitivity and fidelity. Paraffin 60-72 tumor protein p53 Homo sapiens 43-46 8818255-2 1996 This is a longitudinal study of p53 expression in fixed, paraffin-embedded tissue from 3 patients with multiple, recurrent, squamous cell carcinomas of floor of mouth (n = 4, 4, 3). Paraffin 57-65 tumor protein p53 Homo sapiens 32-35 8666362-4 1996 Paraffin-embedded tissue sections were immunostained with monoclonal antibody to p53 and MDM2 proteins. Paraffin 0-8 tumor protein p53 Homo sapiens 81-84 10330207-3 1996 Mutational analysis of p53 was performed retrospectively by means of topographic genotyping (TG), using formalin-fixed, paraffin-embedded tissue of the primary and recurrent tumor. Paraffin 120-128 tumor protein p53 Homo sapiens 23-26 8608838-9 1996 p53 protein overexpression was determined immunohistochemically from paraffin sections of the original primary tumour and resected metastases. Paraffin 69-77 tumor protein p53 Homo sapiens 0-3 8640663-3 1996 METHODS: The expression of p53 was studied immunohistochemically in 200 gastric carcinomas using paraffin embedded surgical specimens and endoscopic biopsies. Paraffin 97-105 tumor protein p53 Homo sapiens 27-30 8606380-5 1996 METHODS: Immunohistochemical analysis with a monoclonal antibody (DO7) specific for p53 protein was used to detect expression of the protein in formalin-fixed, paraffin-embedded tumor samples from 69 head and neck cancer patients treated with definitive local therapy (surgery and/or radiotherapy) between January 1980 and October 1983 at The University of Texas M. D. Anderson Cancer Center. Paraffin 160-168 tumor protein p53 Homo sapiens 84-87 8611406-2 1996 Expression of P53 protein was detected by an immunohistochemical approach using the monoclonal antibody PAb1801 on paraffin-embedded sections of tumours obtained surgically from 102 stage II - IIIa patients with non-small-cell lung cancer (52 squamous cell carcinomas, 50 adenocarcinomas). Paraffin 115-123 tumor protein p53 Homo sapiens 14-17 8767868-3 1996 Expression of p53 protein was examined by immunohistochemistry in paraffin-embedded sections. Paraffin 66-74 tumor protein p53 Homo sapiens 14-17 8835823-1 1996 The expression of p53 protein was studied in odontogenic keratocysts (OKC, 11 solitary, 5 recurrent and 6 NBCCS cysts), radicular (RC, n = 5) and dentigerous (DC, n = 5) cysts, using a panel of antibodies to p53 (clone BP53-12, clone 1801 and polyclonal CM1) and a sensitive biotin-streptavidin method on paraffin embedded sections. Paraffin 305-313 tumor protein p53 Homo sapiens 18-21 8695259-1 1996 The expression of mutated p53 protein was studied in paraffin-embedded, formalin-fixed tumour specimens from 183 women with endometrial carcinoma. Paraffin 53-61 tumor protein p53 Homo sapiens 26-29 8925599-3 1996 We used a set of 6 different antibodies against p53, suitable for paraffin section (PAb1801, PAb240, DO-1, DO-7, BP53-12, CM-1). Paraffin 66-74 tumor protein p53 Homo sapiens 48-51 8626117-5 1996 Immunohistochemical staining for the p53 protein with monoclonal antibody PAb 1801 was performed in the paraffin-embedded tissue of each case to screen for p53 overexpression. Paraffin 104-112 tumor protein p53 Homo sapiens 37-40 8691320-1 1996 To examine the expression of p53 protein and gene alterations in oral epithelial lesions including epithelial dysplasias and primary squamous cell carcinomas, immunohistochemical and temperature gradient gel electrophoresis (TGGE) methods were applied to formalin-fixed and paraffin-embedded tissues. Paraffin 274-282 tumor protein p53 Homo sapiens 29-32 8711933-3 1996 In this study, we investigated the immunohistochemical expression of p53 in formalin fixed paraffin embedded archival specimens of 36 extrahepatic bile duct cancers in which p53 expression was found in eighteen (50%) cases. Paraffin 91-99 tumor protein p53 Homo sapiens 69-72 8778328-2 1996 This immunohistochemical study on formalin-fixed, paraffin-embedded material found p53 expression in 43 per cent (n = 51) of carcinomas using a monoclonal antibody (DO-1), whereas no immunoreactivity for p53 was present in tumour-associated non-neoplastic gastric mucosa or tumour stroma. Paraffin 50-58 tumor protein p53 Homo sapiens 83-86 8600043-4 1996 Ki67 (MIB-1) and p53 monoclonal antibodies (active on paraffin embedded tissues) provide insight into nuclear proliferation and control, respectively. Paraffin 54-62 tumor protein p53 Homo sapiens 17-20 8634783-0 1995 A universal method for the mutational analysis of K-ras and p53 gene in non-small-cell lung cancer using formalin-fixed paraffin-embedded tissue. Paraffin 120-128 tumor protein p53 Homo sapiens 60-63 8801209-5 1996 The expression of p53 protein was detected immunohistochemically in paraffin wax embedded liver specimens, after microwave pretreatment. Paraffin 68-80 tumor protein p53 Homo sapiens 18-21 8832944-4 1996 OBJECTIVE AND METHODS: The aim of the present study is to examine the frequency of immunohistochemically detectable p53 protein by using two monoclonal antibodies (D07 and BP53-12) in 8 cases of formalin-fixed and paraffin-embedded specimens of verrucous skin carcinoma. Paraffin 214-222 tumor protein p53 Homo sapiens 116-119 8954175-1 1996 The polymerase chain reaction and HaeIII enzymatic digestion were used to study the seventh exon of the p53 gene in 29 primary and recurrent hepatomas in paraffin-embedded samples from 11 patients. Paraffin 154-162 tumor protein p53 Homo sapiens 104-107 8920221-0 1996 Immunohistochemical detection of P53 overexpression in paraffin wax-embedded squamous cell carcinomas of cattle, horses, cats and dogs. Paraffin 55-67 tumor protein p53 Homo sapiens 33-36 9431695-0 1996 Immunoreactivity of new antibodies anti-p53 and anti-MDM-2 in paraffin embedded tissue samples. Paraffin 62-70 tumor protein p53 Homo sapiens 40-43 9431695-1 1996 Detection of various epitopes of the p53 and MDM-2 proteins, using new antibodies was performed on formalin-fixed and paraffin-embedded tissue samples from breast cancer and compared with results obtained using well-characterized antibodies. Paraffin 118-126 tumor protein p53 Homo sapiens 37-40 8732103-0 1996 [Detection of point mutation of p53 gene by silver staining PCR-SSCP in paraffin-embedded malignant fibrous histiocytoma]. Paraffin 72-80 tumor protein p53 Homo sapiens 32-35 8732103-1 1996 Silver staining PCR-SSCP method was used to detect point mutation of p53 gene in paraffin-embedded malignant fibrous histiocytoma (MFH) tissues. Paraffin 81-89 tumor protein p53 Homo sapiens 69-72 8599466-1 1995 The accumulation of p53 protein was studied immunohistochemically on paraffin-embedded sections of 26 Spitz nevi (SNs), 26 primary invasive cutaneous malignant melanomas (MMs), 20 metastases of MM, and 17 ordinary compound nevi (CNs), using monoclonal antibody BP53-12. Paraffin 69-77 tumor protein p53 Homo sapiens 20-23 8599466-4 1995 We believe that the immunohistochemical detection of p53 protein with the use of monoclonal antibodies such as BP53-12 on paraffin sections, especially when strong nuclear reactivity is demonstrated, may prove to be an adjunctive tool in the histopathologic differentiation of MM from SN. Paraffin 122-130 tumor protein p53 Homo sapiens 53-56 8640471-0 1995 Immunohistochemical detection of p53 overexpression in formalin-fixed, paraffin-embedded sections of endometrial carcinoma. Paraffin 71-79 tumor protein p53 Homo sapiens 33-36 8640471-2 1995 However using CM1 and an enhanced method based on microwave heating and protease digestion enabled the immunohistochemical detection of p53 overexpression in formalin-fixed, paraffin-embedded sections of endometrial carcinoma, although very few positive stainings were obtained without such an enhanced treatment. Paraffin 174-182 tumor protein p53 Homo sapiens 136-139 8640471-7 1995 CONCLUSION: Enhanced immunodetection of p53 in paraffin-embedded tissues will provide a useful alternative to the usual fresh-tissue assay. Paraffin 47-55 tumor protein p53 Homo sapiens 40-43 9815924-1 1995 Immunohistochemical (IHC) staining for p53 protein nuclear expression was evaluated in archival paraffin-embedded radical prostatectomy specimens from 139 patients with clinically localized prostate cancer followed up from 1 to 8 (mean, 4) years. Paraffin 96-104 tumor protein p53 Homo sapiens 39-42 8751332-4 1995 To explore the differences in the expression of p53 protein in these two tumor types, we performed immunohistochemistry on 10 conventional pulmonary adenocarcinomas and 12 bronchioalveolar carcinomas on formalin-fixed and paraffin-embedded material, using the commercially available monoclonal antibody against the mutant p53 protein. Paraffin 222-230 tumor protein p53 Homo sapiens 48-51 7477163-0 1995 More on p53 antigen loss in stored paraffin slides. Paraffin 35-43 tumor protein p53 Homo sapiens 8-11 8822113-1 1995 The expression of p53 and bcl-2 was immunohistochemically investigated in 61 formalin-fixed, paraffin-embedded invasive breast carcinomas. Paraffin 93-101 tumor protein p53 Homo sapiens 18-21 8745486-2 1995 METHODS: Expression of p53 was studied by immunohistochemistry in paraffin sections from 23 normal endometrium, 44 endometrial hyperplasia and 103 endometrial carcinoma. Paraffin 66-74 tumor protein p53 Homo sapiens 23-26 7560890-0 1995 Detection of P53 expression and S-phase cell fraction in paraffin-embedded tissue by a double-labeling technique. Paraffin 57-65 tumor protein p53 Homo sapiens 13-16 7579380-6 1995 The results of immunohistochemistry analysis using a monoclonal anti-p53 antibody on paraffin-embedded specimens were compared with the SSCP data, the tumor karyotypes, and clinical course of each patient. Paraffin 85-93 tumor protein p53 Homo sapiens 69-72 8697990-7 1995 Immunohistochemistry (ABC method) on section of paraffin embedded tissue is a reliable method for detecting c-myc and p53 gene expression in HCC. Paraffin 48-56 tumor protein p53 Homo sapiens 118-121 8630898-2 1995 METHODS: p53 protein expression was studied by immunohistochemistry from paraffin embedded tissue in a series of 136 patients with malignant ovarian epithelial tumors. Paraffin 73-81 tumor protein p53 Homo sapiens 9-12 7472782-3 1995 p53 expression was evaluated by immunohistochemistry in formalin-fixed, paraffin-embedded tissue from 23 leiomyosarcomas, 10 tumours of uncertain malignant potential (TUMPs), and 18 leiomyomas. Paraffin 72-80 tumor protein p53 Homo sapiens 0-3 7490678-0 1995 Detection of loss of heterozygosity of p53 gene in paraffin-embedded breast cancers by non-isotopic PCR-SSCP. Paraffin 51-59 tumor protein p53 Homo sapiens 39-42 7490678-1 1995 A rapid non-isotopic PCR-SSCP (polymerase chain reaction-single-stranded conformation polymorphism) method was developed in this study to detect polymorphism and loss of heterozygosity (LOH) of p53 in formalin-fixed and paraffin-embedded samples of normal breast tissue and of breast cancer. Paraffin 220-228 tumor protein p53 Homo sapiens 194-197 8575232-2 1995 Quantitative analysis of P53 protein expression was performed on paraffin-embedded tissues from 55 smooth muscle tumors of the gastrointestinal tract, using immunofluorescence and flow cytometry. Paraffin 65-73 tumor protein p53 Homo sapiens 25-28 8771158-3 1995 Tumor p53 protein expression was detected by means of immunohistochemistry using the monoclonal antibody D07 on formalin fixed paraffin-embedded tissue sections. Paraffin 127-135 tumor protein p53 Homo sapiens 6-9 8771158-7 1995 Our study suggested that the immunohistochemical assay was a simple method of detection of mutant p53 proteins in routinely processed paraffin-embedded tissues of primary mammary carcinoma. Paraffin 134-142 tumor protein p53 Homo sapiens 98-101 8697968-3 1995 The expression of p53 protein was also immunohistochemically studied in formalin-fixed paraffin embedded specimens of 76 colorectal adenocarcinomas and 112 colorectal polyps. Paraffin 87-95 tumor protein p53 Homo sapiens 18-21 16696005-1 1995 Aim-To investigate the expression of p53 protein in invasive squamous cell carcinoma (SCC) of the larynx and dysplasia in relation to histological grade and tobacco smoking.Method-Paraffin wax embedded tissue sections from 41 cases of invasive SCC of the larynx, 28 cases of dysplasia and 14 control laryngeal biopsy specimens were studied immunohistochemically using two anti-p53 monoclonal antibodies (DO7 and 1801). Paraffin 180-192 tumor protein p53 Homo sapiens 37-40 7657383-0 1995 Detection of TP53 gene mutation in human meningiomas: a study using immunohistochemistry, polymerase chain reaction/single-strand conformation polymorphism and DNA sequencing techniques on paraffin-embedded samples. Paraffin 189-197 tumor protein p53 Homo sapiens 13-17 8625105-5 1995 Immunohistochemical staining for p53 was performed on paraffin embedded tissue of each case. Paraffin 54-62 tumor protein p53 Homo sapiens 33-36 7546049-2 1995 Quantitation of DNA ploidy was determined on Feulgen-stained touch imprints with an image analyzer, whereas localization of the immunohistochemical reaction of Erb-B2 and p53 protein was evaluated in paraffin-embedded tumor specimens with microscopy. Paraffin 200-208 tumor protein p53 Homo sapiens 171-174 7726729-0 1995 A microwave method that enhances detection of aberrant p53 expression in formalin-fixed, paraffin-embedded tissues. Paraffin 89-97 tumor protein p53 Homo sapiens 55-58 7739705-0 1995 p53 antigen loss in stored paraffin slides. Paraffin 27-35 tumor protein p53 Homo sapiens 0-3 7607381-2 1995 It is possible to demonstrate the presence of mutant p 53 protein types in tumour cell nuclei by applying immunohistochemical procedures to paraffin sections (Clon DO 1, Dianova). Paraffin 140-148 tumor protein p53 Homo sapiens 53-57 7615358-2 1995 Mutations in exons 5 through 8 of the p53 gene were identified by a denaturing gradient gel electrophoresis (DGGE) assay and cycle sequencing, whereas p53 protein accumulation was detected in paraffin-embedded tissue by immunostaining using 2 different murine monoclonal antibodies (MAbs) (BP53-12 and DO7). Paraffin 192-200 tumor protein p53 Homo sapiens 151-154 7726729-10 1995 CONCLUSIONS: Microwave pretreatment in conjunction with the use of either PAb1801 or DO7 is highly efficacious in the immunohistochemical detection of aberrant p53 expression in formalin-fixed, paraffin-embedded tissues. Paraffin 194-202 tumor protein p53 Homo sapiens 160-163 7620345-11 1995 We demonstrate that antigen retrieval techniques increase p53 immunoreactivity in paraffin embedded melanocytic tissues. Paraffin 82-90 tumor protein p53 Homo sapiens 58-61 7576299-1 1995 The expression of the p53 protein (p53) was compared with those of several oncogenes including c-fos (Fos), c-jun (Jun), and epidermal growth factor receptor (EGFR1) using immunohistochemistry in frozen and paraffin-embedded sections of 25 basal cell carcinomas (BCCs) to find out any correlation between p53 and oncogenes in the pathogenesis of human BCC. Paraffin 207-215 tumor protein p53 Homo sapiens 22-25 7860045-1 1995 Several studies of benign breast lesions using methacran-fixed, paraffin-embedded tissues and cytological preparations have suggested that p53 accumulation in these lesions as detected by immunohistochemical (IHC) staining is rare to absent. Paraffin 64-72 tumor protein p53 Homo sapiens 139-142 7796648-2 1995 Detection of overexpression of p53 protein was carried out by immunohistochemical staining (IHS) using the monoclonal antibody DO-1 on paraffin sections. Paraffin 135-143 tumor protein p53 Homo sapiens 31-34 7556591-6 1995 Nuclear p53 immunoreactivity was assessed using a monoclonal antibody, DO-1, on Formalin-fixed paraffin-embedded specimens. Paraffin 95-103 tumor protein p53 Homo sapiens 8-11 7734516-12 1995 Immunohistochemical analysis of the same paraffin-embedded sections showed that infectivity and level of expression of p53 in lung tissue were dose-dependent. Paraffin 41-49 tumor protein p53 Homo sapiens 119-122 7860046-5 1995 Image analysis showed that the surfaces positive with anti-p53 and the staining intensity were decreased (P < .01) on paraffin sections. Paraffin 121-129 tumor protein p53 Homo sapiens 59-62 7852482-5 1995 Putative p53 mutations were detected by immunohistochemistry on paraffin-embedded sections using polyclonal CM-1 and monoclonal DO-7 and PAb1801 antibodies. Paraffin 64-72 tumor protein p53 Homo sapiens 9-12 11578436-3 1994 We also examined the expression of p53 in paraffin tissues by immunohistochemical staining and determined the incidence of human papillomavirus (HPV) sequences in the same tissues by multitype PCR analysis to correlate them to the allelic loss on chromosome 17p13 and p53 mutation. Paraffin 42-50 tumor protein p53 Homo sapiens 35-38 7745116-2 1995 METHODS: The expression of p53, Rb and bcl-2 proteins in paraffin wax embedded tonsil tissue sections was detected by immunohistochemistry using an (APAAP) technique following microwave irradiation. Paraffin 57-69 tumor protein p53 Homo sapiens 27-30 7819040-5 1995 The immunohistochemical detection of mutations of the p53 gene has been demonstrated to be a reliable, easily performed and thereby widely available technique for the investigation of fresh-frozen or paraffin-embedded tumour specimens. Paraffin 200-208 tumor protein p53 Homo sapiens 54-57 7704245-1 1995 The expression of p53 protein was studied in formalin-fixed paraffin-embedded specimens of 41 well-differentiated adenocarcinomas of the gall-bladder, six cases of acute cholecystitis and 23 cases of chronic cholecystitis, using a monoclonal p53 (PAb 1801) antibody and streptavidin-biotin. Paraffin 60-68 tumor protein p53 Homo sapiens 18-21 7720108-0 1994 [Detection of point mutation of p53 gene by non-isotopic PCR-SSCP in paraffin-embedded breast cancer tissue]. Paraffin 69-77 tumor protein p53 Homo sapiens 32-35 7720108-1 1994 Non-isotopic PCR-SSCP method was used to detect point mutation of p53 gene in paraffin-embedded breast cancers. Paraffin 78-86 tumor protein p53 Homo sapiens 66-69 7942634-1 1994 Immunohistochemical staining for the p53 protein was performed in microwave-fixed, paraffin-embedded sections of normal, premalignant and malignant tissues of the female genital tract using a monoclonal antibody, PAb 1801. Paraffin 83-91 tumor protein p53 Homo sapiens 37-40 7959668-1 1994 Immunohistochemical expression of the cellular phosphoprotein p53 was investigated in archival, formalin-fixed, and paraffin-embedded surgical breast tissue specimens from 543 patients using the polyclonal antibody CM-1. Paraffin 116-124 tumor protein p53 Homo sapiens 62-65 7876377-2 1994 METHODS: Immunocytochemistry for p53 was performed in 65 specimens of formalin fixed, paraffin wax embedded cervical tissue using a polyclonal antibody against recombinant p53. Paraffin 86-98 tumor protein p53 Homo sapiens 33-36 7527509-5 1994 In the second part of the study, accumulation of p53 protein was determined in 42 archival paraffin-embedded specimens from oral leukoplakia and correlated with the degree of epithelial dysplasia. Paraffin 91-99 tumor protein p53 Homo sapiens 49-52 7823248-2 1994 Genomic p53 was amplified by the polymerase chain reaction (PCR) from paraffin-embedded sections of tumour and non-tumour tissue. Paraffin 70-78 tumor protein p53 Homo sapiens 8-11 7962351-5 1994 Putative p53 mutations were detected by immunohistochemistry on paraffin-embedded sections using polyclonal CM-1 and monoclonal DO-7 and PAb1801 antibodies. Paraffin 64-72 tumor protein p53 Homo sapiens 9-12 7848689-1 1994 BACKGROUND AND METHODS: p53 protein expression was studied immunohistochemically in 73 colorectal adenocarcinomas, using monoclonal antibody D07 in alcohol fixed, paraffin embedded tissue. Paraffin 163-171 tumor protein p53 Homo sapiens 24-27 7882774-1 1994 Quantitative analysis of p53 protein expression was performed on paraffin-embedded tissues from 55 smooth muscle tumors of the gastrointestinal tract by using immunofluorescence and flow cytometry. Paraffin 65-73 tumor protein p53 Homo sapiens 25-28 7922972-6 1994 METHODS: p53 expression in formalin fixed, paraffin embedded samples of 204 patients with primary squamous cell carcinoma of the esophagus, who underwent esophageal resection, were analyzed immunohistochemically with DO-1, a monoclonal antibody that detects wild-type and mutant forms of p53. Paraffin 43-51 tumor protein p53 Homo sapiens 9-12 7580888-2 1994 Sequences of the p53 gene have been obtained from DNA extracted from frozen and formalin-fixed paraffin-embedded cancer tissues. Paraffin 95-103 tumor protein p53 Homo sapiens 17-20 7928628-0 1994 Nuclear accumulation of p53 protein correlates with mutations in the p53 gene on archival paraffin-embedded tissues of human breast cancer. Paraffin 90-98 tumor protein p53 Homo sapiens 24-27 7928628-0 1994 Nuclear accumulation of p53 protein correlates with mutations in the p53 gene on archival paraffin-embedded tissues of human breast cancer. Paraffin 90-98 tumor protein p53 Homo sapiens 69-72 7928628-8 1994 We conclude that the immunohistochemical detection of nuclear p53 protein accumulation is highly associated with p53 gene mutations in archival paraffin-embedded tissues, and that this method is useful for rapid screening of p53 abnormalities. Paraffin 144-152 tumor protein p53 Homo sapiens 62-65 7530851-0 1994 p53 immunolabeling in archival paraffin-embedded tissues: optimal protocol based on microwave heating for eight antibodies on lung carcinomas. Paraffin 31-39 tumor protein p53 Homo sapiens 0-3 21559604-2 1994 Positive staining for p53 protein was detected in 44 of 71 (62%) of these tumors on paraffin-embedded tissue, even in dysplastic areas. Paraffin 84-92 tumor protein p53 Homo sapiens 22-25 7708439-9 1994 P53 was investigated by immunohistochemistry, using a monoclonal anti-p53 antibody (DO-7), on formalin fixed, paraffin embedded tissue. Paraffin 110-118 tumor protein p53 Homo sapiens 0-3 7913577-10 1994 Both p53 protein and p185 antibodies work well on routine, formalin-fixed, paraffin-embedded tissue and are easily used in routine diagnostic procedures. Paraffin 75-83 tumor protein p53 Homo sapiens 5-8 7808790-3 1994 At present, our aim was, firstly, to see which were the best technical conditions for detection of p53 in the available paraffin-embedded tumor specimens, using several antibodies, specific for various epitopes; secondly, to investigate if some relation might exist between this expression and the histological features of these tumors. Paraffin 120-128 tumor protein p53 Homo sapiens 99-102 7931842-4 1994 We examined 62 endoscopic oesophageal biopsies and 36 oesophageal resections for p53 overexpression using the monoclonal antibody DO-7 on paraffin-embedded tissue. Paraffin 138-146 tumor protein p53 Homo sapiens 81-84 8200229-3 1994 METHODS: IgG1 monoclonal antibody to human p53 protein (PAb 1801) was used to detect p53 in formalin-fixed, paraffin-embedded archival tumors resected from 84 patients with tumor limited to the bowel wall. Paraffin 108-116 tumor protein p53 Homo sapiens 85-88 7805554-1 1994 The monoclonal antibody to p53 and a rapid immunohistochemical method were used in this study to detect p53 expression in 50 paraffin-embedded breast cancers and 7 benign hyperplastic diseases of breast. Paraffin 125-133 tumor protein p53 Homo sapiens 104-107 8162251-3 1994 We have developed a method utilizing polymerase chain reaction (PCR) for the direct analysis of p53 mutation by single-strand conformation polymorphism (SSCP) and by direct DNA sequencing of the p53 gene using a single 10-microns paraffin-embedded tissue section. Paraffin 230-238 tumor protein p53 Homo sapiens 195-198 7909221-5 1994 Positive staining for both p53 and HER-2/neu in paraffin-embedded tissues indicates an underlying genetic abnormality: point mutations in the p53 gene and amplification of the HER-2/neu gene. Paraffin 48-56 tumor protein p53 Homo sapiens 27-30 7909221-5 1994 Positive staining for both p53 and HER-2/neu in paraffin-embedded tissues indicates an underlying genetic abnormality: point mutations in the p53 gene and amplification of the HER-2/neu gene. Paraffin 48-56 tumor protein p53 Homo sapiens 142-145 8012775-2 1994 PATIENTS AND METHODS: The immunohistochemical status of the p53 protein in 28 pT1 primary bladder cancers was determined on frozen tissue and archival paraffin block sections using three primary antibodies (CM-1, PAB1801 and D07). Paraffin 151-159 tumor protein p53 Homo sapiens 60-63 8012776-4 1994 p53 expression was determined by immuno-histochemistry on paraffin embedded sections and flow cytometry was performed on cell suspensions derived from the same blocks. Paraffin 58-66 tumor protein p53 Homo sapiens 0-3 8133533-4 1994 METHODS: The expression of Bcl-2 and p53 was detected by immunohistochemistry on paraffin-embedded sections from 283 node-negative resectable breast cancers treated with local-regional therapy alone until relapse. Paraffin 81-89 tumor protein p53 Homo sapiens 37-40 8043159-9 1994 Since p53 (PAb 1801) expression can withstand formalin fixation and pepsin treatment of paraffin-embedded tissues, flow cytometric analysis of archival specimens is feasible, and clinical correlations such as these may be carried out in retrospective studies of other tumors. Paraffin 88-96 tumor protein p53 Homo sapiens 6-9 8156496-3 1994 METHODS: The status of the p53 gene was studied by immunohistochemistry of formalin-fixed paraffin-embedded biopsy samples from 72 patients with atypical endometrial hyperplasia or endometrioid carcinoma who underwent hysterectomy immediately after biopsy and from 5 patients with benign endometria. Paraffin 90-98 tumor protein p53 Homo sapiens 27-30 8162251-4 1994 We applied this method to screen for p53 gene mutations in exons 5-8 in human gliomas utilizing paraffin-embedded tissues. Paraffin 96-104 tumor protein p53 Homo sapiens 37-40 7719291-0 1994 p53 mutant gene expression in paraffin embedded and fresh frozen tissue of different urogenital tumors. Paraffin 30-38 tumor protein p53 Homo sapiens 0-3 21607363-0 1994 Immunohistochemical expression of p53 paraffin sections from colon-cancer specimens - comparison between 2 new monoclonal-antibodies. Paraffin 38-46 tumor protein p53 Homo sapiens 34-37 21607363-2 1994 We examined the immunohistochemical expression of p53 in 40 paraffin-embedded samples obtained from colon carcinomas using the two new MAbs DO-1 and DO-7. Paraffin 60-68 tumor protein p53 Homo sapiens 50-53 8311654-4 1994 Positive nuclear p53 immunoreactivity was detected in 37% of paraffin-embedded primary tumors (21.8% in the Japanese group, 52.6% in the American group) and in 50% of xenotransplanted carcinoma cell lines. Paraffin 61-69 tumor protein p53 Homo sapiens 17-20 8008749-1 1994 p53 expression in formalin-fixed, paraffin-embedded archival specimens of intrahepatic cholangiocarcinoma: retrieval of p53 antigenicity by microwave oven heating of tissue sections. Paraffin 34-42 tumor protein p53 Homo sapiens 0-3 8008749-4 1994 We investigated expression of p53 in formalin-fixed, paraffin-embedded archival specimens of 40 CCs (22 autopsy cases and 18 surgical cases) by immunohistochemistry using four antibodies (PAb1801, DO-7, BP53-12, CM1). Paraffin 53-61 tumor protein p53 Homo sapiens 30-33 8116572-1 1994 The authors had previously reported that the accumulation of p53 is clearly demonstrable in microwave-fixed, paraffin-embedded sections of colorectal carcinomas. Paraffin 109-117 tumor protein p53 Homo sapiens 61-64 8008749-10 1994 These results shows that a pretreatment of tissue sections by microwave oven heating is a very good method for demonstrating p53 protein in formalin-fixed, paraffin-embedded archival materials and that DO-7, BP53-12, and CM1 are useful antibodies for detection of p53 in formalin-fixed, paraffin-embedded archival materials. Paraffin 156-164 tumor protein p53 Homo sapiens 125-128 8032216-5 1994 We found point mutations in 25% (9 of 36) of cultured melanomas and 0% in 34 fresh melanoma biopsies; however, increased p53 expression was found in 42% of paraffin-embedded melanoma specimens and 7% of benign lesions. Paraffin 156-164 tumor protein p53 Homo sapiens 121-124 8293408-5 1994 Immunostaining for mutant p53 proteins was performed on tissues fixed in formaldehyde solution and embedded in paraffin; the tissues were from these 44 astrocytomas and another 103 astrocytomas obtained from archival material. Paraffin 111-119 tumor protein p53 Homo sapiens 26-29 7506924-1 1994 The availability of antibodies which recognise p53 protein in paraffin-embedded tissue has created the opportunity to use immunohistochemistry to study the expression of p53 in a wide variety of clinical material. Paraffin 62-70 tumor protein p53 Homo sapiens 47-50 21573436-4 1993 Expression of p53 protein and P-170 glycoprotein was detected by immunohistochemistry using the PAb1801 and C219 monoclonal antibody respectively, on sections from paraffin-embedded tumor biopsies. Paraffin 164-172 tumor protein p53 Homo sapiens 14-17 7931822-1 1994 Immunocytochemical detection of p53 protein products in paraffin sections is possible with a number of antisera, monoclonal and polyclonal. Paraffin 56-64 tumor protein p53 Homo sapiens 32-35 7909153-9 1994 This study demonstrates the use of paraffin sections in the analysis of p53 alterations in neoplasia. Paraffin 35-43 tumor protein p53 Homo sapiens 72-75 8165016-0 1994 Paraffin section p53 protein immunohistochemistry in neuroectodermal tumors. Paraffin 0-8 tumor protein p53 Homo sapiens 17-20 8186888-0 1994 Polymerase chain reaction-single strand conformation polymorphism analysis of the p53 gene in paraffin-embedded surgical material from human renal cell carcinomas. Paraffin 94-102 tumor protein p53 Homo sapiens 82-85 8158458-1 1993 The expression of the nuclear protein p53 in oligodendrogliomas was investigated by immunohistochemistry, using a monoclonal anti-p53 antibody (DO-7) on formalin-fixed, paraffin-embedded material in 84 histologically verified cases, and compared with the histopathological grade and survival. Paraffin 169-177 tumor protein p53 Homo sapiens 38-41 7692889-0 1993 Retrieval of p53 protein in paraffin-embedded head and neck tumor tissues. Paraffin 28-36 tumor protein p53 Homo sapiens 13-16 7692889-1 1993 OBJECTIVES: To improve the detection of p53 protein in formalin-fixed, paraffin-embedded head and neck tumor tissues. Paraffin 71-79 tumor protein p53 Homo sapiens 40-43 7692889-8 1993 RESULTS: An antigen retrieval method facilitated the unmasking of previously inaccessible p53 antigenic determinants in formalin-fixed, paraffin-embedded tissues. Paraffin 136-144 tumor protein p53 Homo sapiens 90-93 7692889-11 1993 CONCLUSIONS: Antigen retrieval method in formalin-fixed, paraffin-embedded tissue demonstrated a significant increase in p53 immunostaining. Paraffin 57-65 tumor protein p53 Homo sapiens 121-124 8244324-3 1993 We describe the localization of p53 protein in association with HPV in paraffin sections of a spectrum of benign, dysplastic, and malignant anogenital squamous epithelia using immunohistochemical and in situ hybridization techniques. Paraffin 71-79 tumor protein p53 Homo sapiens 32-35 8252480-5 1993 METHODS: Tumor tissues of 156 patients with primary invasive breast cancer were analyzed immunohistochemically for the presence of p53 protein in paraffin-embedded material. Paraffin 146-154 tumor protein p53 Homo sapiens 131-134 8238731-3 1993 The goal of this study was to determine the frequency and relationship of p53 gene alterations and hepatitis B in formalin-fixed, paraffin-embedded HCCs resected in the United States. Paraffin 130-138 tumor protein p53 Homo sapiens 74-77 7901994-13 1993 We conclude that the immunohistochemical detection of p53 nuclear accumulation in formalin-fixed, paraffin-embedded tissue is highly associated with mutations in the p53 gene; this association has now been demonstrated in a large number of tumors. Paraffin 98-106 tumor protein p53 Homo sapiens 54-57 7901994-13 1993 We conclude that the immunohistochemical detection of p53 nuclear accumulation in formalin-fixed, paraffin-embedded tissue is highly associated with mutations in the p53 gene; this association has now been demonstrated in a large number of tumors. Paraffin 98-106 tumor protein p53 Homo sapiens 166-169 8248107-1 1993 p53 overexpression was studied immunohistochemically in paraffin-embedded bone marrow biopsies using a recently described technique for antigen retrieval based on microwave oven heating of paraffin sections. Paraffin 56-64 tumor protein p53 Homo sapiens 0-3 7508232-3 1993 Using streptavidin-biotin complex method, p53 was identified in 33% of dysplastic squamous lesions, 50% of squamous cell carcinomas (SCCs) and 36% of basal cell carcinomas (BCCs) on frozen section, whereas 25% of dysplastic squamous lesions, 40% of SCCs, and 32% of BCCs showed p53 positivity on paraffin-embedded sections. Paraffin 296-304 tumor protein p53 Homo sapiens 42-45 8283348-6 1993 Overexpression of the p53 protein was found by immunostaining of sections from formalin-fixed, paraffin-embedded material in 55 per cent (51/92) of the tumours. Paraffin 95-103 tumor protein p53 Homo sapiens 22-25 8283352-0 1993 Immunocytochemical p53 detection by microwave oven heating of routinely formalin-fixed paraffin sections. Paraffin 87-95 tumor protein p53 Homo sapiens 19-22 8248107-1 1993 p53 overexpression was studied immunohistochemically in paraffin-embedded bone marrow biopsies using a recently described technique for antigen retrieval based on microwave oven heating of paraffin sections. Paraffin 189-197 tumor protein p53 Homo sapiens 0-3 8363836-0 1993 A method for PCR sequencing of the p53 gene from a single 10-microns frozen or paraffin-embedded tissue section. Paraffin 79-87 tumor protein p53 Homo sapiens 35-38 8112175-1 1993 Expression of P53 protein was detected immunohistochemically in formalin-fixed, paraffin-embedded tissues from 67 patients with bladder carcinoma and 6 normal bladder controls. Paraffin 80-88 tumor protein p53 Homo sapiens 14-17 8347496-1 1993 The expression of p53 protein was immunohistochemically studied in formalin-fixed paraffin-embedded biopsy specimens of 203 colorectal carcinomas by use of a monoclonal antibody specific for the p53 protein. Paraffin 82-90 tumor protein p53 Homo sapiens 18-21 8496982-5 1993 METHODS: Expression of mutant p53 protein was detected by an immunohistochemical technique using a 1:50 dilution of PAb1801 monoclonal antibody on paraffin-embedded tumor specimens obtained from 256 axillary lymph node-negative breast cancer patients, with long-term follow-up (median, 72 months). Paraffin 147-155 tumor protein p53 Homo sapiens 30-33 8415589-1 1993 The p53 gene was examined in a series of formalin-fixed paraffin-embedded astrocytic neoplasms of various types by polymerase chain reaction (PCR), single-strand conformation polymorphism analysis (SSCP), and direct sequencing of amplified DNA. Paraffin 56-64 tumor protein p53 Homo sapiens 4-7 8392521-3 1993 METHODS: The presence and localisation of the p53 protein in formalin fixed, paraffin wax embedded specimens of anal squamous epithelium (normal and neoplastic) was examined using immunohistochemical staining with a panel of two monoclonal antibodies (DO-1, DO-7) and one polyclonal antibody (CM-1). Paraffin 77-89 tumor protein p53 Homo sapiens 46-49 32370448-2 1993 In the present study, p53 immunoreactivity was investigated in formalin-fixed, paraffin-embedded tissues from human and animal pituitary tumors, using the avidin-biotin-peroxidase complex technique. Paraffin 79-87 tumor protein p53 Homo sapiens 22-25 7684193-2 1993 Immunohistochemical staining with a monoclonal antibody (DO-7) and a polyclonal antibody (CM-1) to p53 protein (both wild type and mutant) on formalin-fixed paraffin sections showed a strong correlation with malignancy grade. Paraffin 157-165 tumor protein p53 Homo sapiens 99-102 8382897-6 1993 In this study, we stained paraffin sections from eight samples of gliosarcomas from seven patients with an antibody to p53. Paraffin 26-34 tumor protein p53 Homo sapiens 119-122 8483890-0 1993 p53 protein immunostaining in routinely processed paraffin-embedded sections. Paraffin 50-58 tumor protein p53 Homo sapiens 0-3 8483890-3 1993 We report a technique for immunostaining of the altered p53 protein in routinely processed paraffin-embedded tissue sections, comparing this method with a frozen-section immunostain method for concordance. Paraffin 91-99 tumor protein p53 Homo sapiens 56-59 8483890-9 1993 We propose that this new paraffin-embedded section immunostaining method will be of value as a screening technique for the investigation of p53 mutations in archived human tumors. Paraffin 25-33 tumor protein p53 Homo sapiens 140-143 8101139-3 1993 We used newly-developed PCR-RFLP technique to detect the point mutation of the 7th exon of p53 gene in fresh and paraffin embedded tissues of hepatocellular carcinoma in studying 40 cases. Paraffin 113-121 tumor protein p53 Homo sapiens 91-94 7688167-1 1993 Expression of p53 protein was examined in 67 cases of primary transitional cell carcinoma of the bladder and 6 normal controls using an immunohistochemical method on paraffin sections. Paraffin 166-174 tumor protein p53 Homo sapiens 14-17 8384406-1 1993 Striking differences were found between different histological types of breast cancer when 263 invasive breast carcinomas were tested for nuclear p53 accumulation in formaldehyde-fixed paraffin sections. Paraffin 185-193 tumor protein p53 Homo sapiens 146-149 8384408-1 1993 The spectrum of p53 gene mutations was determined in formalin-fixed, paraffin-embedded samples of small-cell lung cancer derived from 28 patients. Paraffin 69-77 tumor protein p53 Homo sapiens 16-19 8434637-0 1993 Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid. Paraffin 45-53 tumor protein p53 Homo sapiens 13-16 8434637-1 1993 With the aid of a newly developed target unmasking fluid (TUF), p53 overexpression was visualized by immunohistochemistry on recent and archival paraffin-embedded tissue samples of colon, stomach, and pancreas neoplasms. Paraffin 145-153 tumor protein p53 Homo sapiens 64-67 8506620-2 1993 Using a microwave oven heating method, we have detected over-expression of p53 in buffered-formalin fixed, paraffin-embedded sections of oesophageal carcinomas immunohistochemically and examined the relationship between the p53 over-expression and postoperative survival. Paraffin 107-115 tumor protein p53 Homo sapiens 75-78 8433213-0 1993 Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies. Paraffin 55-63 tumor protein p53 Homo sapiens 36-39 8318988-4 1993 Here we describe a simple technique for DNA isolation that permits mutational analysis of p53 from minimal amounts of paraffin-embedded archival tissue samples. Paraffin 118-126 tumor protein p53 Homo sapiens 90-93 1295480-7 1992 Mutant p53-protein, as detected by immunohistochemistry in paraffin embedded tumor tissue, does not appear to influence metastasis-free survival in patients with breast cancer. Paraffin 59-67 tumor protein p53 Homo sapiens 7-10 1484319-0 1992 p53 immunostaining in the distinction between benign and malignant mesothelial proliferations using formalin-fixed paraffin sections. Paraffin 115-123 tumor protein p53 Homo sapiens 0-3 1484319-2 1992 The object of this study was to examine the frequency of immunohistochemically detectable p53 overexpression in routinely processed, paraffin-embedded tissue from pleural mesotheliomas and from pleura showing reactive mesothelial hyperplasia, using a polyclonal antibody to formalin-resistant p53 epitopes, and to consider the diagnostic utility of this antibody in the distinction between mesothelioma and reactive mesothelium in pleural biopsy specimens. Paraffin 133-141 tumor protein p53 Homo sapiens 90-93 1484319-6 1992 In conclusion, this study suggests that p53 immunostaining can help to distinguish epithelial or biphasic mesothelioma from reactive mesothelial hyperplasia in formalin-fixed, paraffin-embedded pleural biopsy specimens. Paraffin 176-184 tumor protein p53 Homo sapiens 40-43 1444195-3 1992 However, the antibody is effective in AMeX-fixed specimens, thereby permitting the improved morphologic localization of p53 phosphoprotein in paraffin embedded tissue. Paraffin 142-150 tumor protein p53 Homo sapiens 120-123 1408139-6 1992 Five of the six cases showed involvement of the p53 gene as assessed by LOH at the AccII site within the gene, and by immunoreactivity to CM-1, an antibody which recognizes the mutated form of the p53 protein in paraffin-embedded material. Paraffin 212-220 tumor protein p53 Homo sapiens 48-51 1408139-6 1992 Five of the six cases showed involvement of the p53 gene as assessed by LOH at the AccII site within the gene, and by immunoreactivity to CM-1, an antibody which recognizes the mutated form of the p53 protein in paraffin-embedded material. Paraffin 212-220 tumor protein p53 Homo sapiens 197-200 1358781-3 1992 Immunoreactive p53 was observed in the nuclei of tumor cells in 4% paraformaldehyde-fixed, frozen sections (12 of 15) and paraffin-embedded sections (11 of 15), but not in routinely processed (10% formalin-fixed) specimens. Paraffin 122-130 tumor protein p53 Homo sapiens 15-18 1444195-0 1992 Improved immunohistochemical detection of p53 protein in paraffin--embedded tissues reveals elevated levels in most head and neck and lung carcinomas: correlation with clinicopathological parameters. Paraffin 57-65 tumor protein p53 Homo sapiens 42-45 1444195-1 1992 We have analyzed the expression of the p53 tumor suppressor gene in paraffin-embedded sections of normal and malignant head and neck and lung tumors by immunohistochemistry using the PAb 1801 monoclonal antibody (MAb). Paraffin 68-76 tumor protein p53 Homo sapiens 39-42 1319835-9 1992 Also, carcinoma cells with p53 mutations existing within adenoma tissues are detectable by immunostaining, even in formalin-fixed, paraffin-embedded specimens. Paraffin 131-139 tumor protein p53 Homo sapiens 27-30 1520594-1 1992 p53 expression was examined in 55 gastric and 107 colorectal carcinomas with an immunoperoxidase technique, using the polyclonal antibody CM1 on routinely fixed, paraffin embedded tissue. Paraffin 162-170 tumor protein p53 Homo sapiens 0-3 1607637-0 1992 p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry. Paraffin 38-46 tumor protein p53 Homo sapiens 0-3 1619684-9 1992 Protein expression of TP53 was seen by immunostaining of sections from paraffin-embedded material using a mouse monoclonal antibody. Paraffin 71-79 tumor protein p53 Homo sapiens 22-26 1352359-8 1992 Overexpression of the p53 and c-erbB-2 proteins was studied immunohistochemically in archival paraffin-embedded tumor samples, using the CM-1 polyclonal and the TAb 250 monoclonal antibodies, respectively. Paraffin 94-102 tumor protein p53 Homo sapiens 22-25 1607637-3 1992 We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. Paraffin 30-38 tumor protein p53 Homo sapiens 12-15 1607637-6 1992 Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors. Paraffin 26-34 tumor protein p53 Homo sapiens 19-22 1510215-1 1992 A total of 26 specimens of histologically determined, cutaneous Bowen"s disease from 23 patients were investigated for the incidence of mutant p53 protein expression by an indirect immunoperoxidase technique using the monoclonal antiserum CM1 on paraffin-embedded formalin-fixed tissue sections. Paraffin 246-254 tumor protein p53 Homo sapiens 143-146 1317462-3 1992 METHODS: IgG1 monoclonal antibody to human p53 protein (PAb 1801) and immunohistochemical methods were used to detect p53 protein accumulation in archival formalin-fixed, paraffin-embedded, randomly selected carcinomas. Paraffin 171-179 tumor protein p53 Homo sapiens 118-121 1347094-3 1992 Mutations were not found in Ki-ras, but 9 p53 mutations, including 2 deletions, were found in 7 patients by direct DNA sequencing after polymerase chain reaction amplification of DNA from formalin-fixed, paraffin-embedded tissue. Paraffin 204-212 tumor protein p53 Homo sapiens 42-45 1546693-0 1992 Immunohistochemical study of p53 expression in microwave-fixed, paraffin-embedded sections of colorectal carcinoma and adenoma. Paraffin 64-72 tumor protein p53 Homo sapiens 29-32 1737400-4 1992 In the present study, mutations in the p53 gene were investigated by direct sequencing analysis after polymerase chain reaction amplification of exons 5 to 8, using paraffin-embedded primary tumors and cultured cells. Paraffin 165-173 tumor protein p53 Homo sapiens 39-42 1546693-1 1992 Expression of p53, a tumor-suppressor gene product, was studied immunohistochemically in microwave-fixed, paraffin-embedded sections of 84 colorectal carcinomas and 44 adenomas. Paraffin 106-114 tumor protein p53 Homo sapiens 14-17 1546693-9 1992 The immunohistochemical detection of p53 in microwave-fixed, paraffin-embedded sections of colorectal carcinoma and adenoma can provide valuable information about the mechanism of carcinogenesis in colorectal epithelium. Paraffin 61-69 tumor protein p53 Homo sapiens 37-40 1569122-5 1992 Most importantly, they also permit the detection of p53 in archival tumour material that has been conventionally fixed in formalin and embedded in paraffin wax. Paraffin 147-159 tumor protein p53 Homo sapiens 52-55 1413493-1 1992 The expression of the p53 gene product was investigated immunocytochemically in a retrospective series of 164 formalin-fixed paraffin-embedded invasive breast carcinomas with pathologically proven negative lymph nodes. Paraffin 125-133 tumor protein p53 Homo sapiens 22-25 1924172-5 1991 The original paraffin-embedded specimen from which this mutant cell line was established was obtained, and sequence analysis detected the identical mutation in the p53 gene as that seen in the derived cell line. Paraffin 13-21 tumor protein p53 Homo sapiens 164-167 1935458-0 1991 Flow cytometric measurement of p53 protein expression and DNA content in paraffin-embedded tissue from bronchial carcinomas. Paraffin 73-81 tumor protein p53 Homo sapiens 31-34 1935458-3 1991 After immunostaining, the nuclei prepared from paraffin-embedded tissue were stained with propidium iodide for simultaneous measurement of DNA content; 17 of 24 lung cancers were p53 positive. Paraffin 47-55 tumor protein p53 Homo sapiens 179-182 1935458-9 1991 In conclusion, using the monoclonal antibody PAb 1801, p53 is detectable in cell nuclei prepared from paraffin-embedded bronchial carcinoma biopsies. Paraffin 102-110 tumor protein p53 Homo sapiens 55-58 1764370-4 1991 One of the antibodies, CM1, recognises p53 in formalin-fixed, paraffin-embedded archival material and using this reagent we found immunodetectable p53 in 28 of 124 (23%) pancreatic cancers. Paraffin 62-70 tumor protein p53 Homo sapiens 39-42 1923535-2 1991 Immunohistochemical staining with new polyclonal (CM-1) and monoclonal antibodies (BP 53-12 and BP53-24) to p53 on methacarn-fixed paraffin sections showed positive staining in 161 (76%). Paraffin 131-139 tumor protein p53 Homo sapiens 108-111 1680118-3 1991 In this study immunohistochemical staining for p53-protein was performed on frozen- and paraffin-embedded samples from patients with Hodgkin"s (HD) and non-Hodgkin"s lymphomas (NHL). Paraffin 88-96 tumor protein p53 Homo sapiens 47-50