PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 34422362-10 2021 PD-L1 expression differed between different paraffin blocks from the same resected specimen (discordance rate =35.8%, 19/53), and the discordance rate of PD-L1 expression between primary tumours and matched lymph node metastases was 28.6% (14/49). Paraffin 44-52 CD274 molecule Homo sapiens 0-5 34953041-7 2022 PD-L1 expression was highly consistent in different paraffin blocks of the same surgically resected specimen (concordance rate: 86.5%, p = 0.000016) and a moderate consistency (concordance rate: 78.0%, p = 0.000373) for the primary and metastatic lymph node lesion comparison. Paraffin 52-60 CD274 molecule Homo sapiens 0-5 34513680-3 2021 Methods: PD-L1 expression was evaluated using paraffin-embedded specimens from patients with surgically treated high-grade SGC, and it was scored by the tumor proportion score (TPS), combined positive score (CPS), and immune cell (IC) score. Paraffin 46-54 CD274 molecule Homo sapiens 9-14 34178624-3 2021 Materials and Methods: We verify a co-expression of BRAF V600E mutation and PD-L1 high expression more than 50% on formalin-fixed paraffin-embedded tumor sample of a newly diagnosed lung adenocarcinoma patient by immunohistochemistry and BRAF V600E/EGFR/ALK/ROS1 Mutations Detection Kit. Paraffin 130-138 CD274 molecule Homo sapiens 76-81 34206205-4 2021 METHODS: We compared PD-L1 expression in patient-matched frozen (FR) and formalin-fixed paraffin-embedded (FFPE) tissues of breast cancer patients. Paraffin 88-96 CD274 molecule Homo sapiens 21-26 35562908-2 2022 High tumor PD-L1 expression, detected by immunohistochemistry (IHC) typically on formalin-fixed paraffin-embedded (FFPE) histological specimens, is linked to better response. Paraffin 96-104 CD274 molecule Homo sapiens 11-16 35403870-2 2022 Immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded (FFPE) tumor tissue has an established role in detecting PD-L1 expression and in ALK, ROS1, and more recently NTRK testing. Paraffin 46-54 CD274 molecule Homo sapiens 121-126 35589508-5 2022 MATERIALS AND METHODS: ICC for PD-L1 was performed on formalin-fixed paraffin-embedded CBs of MPE and patient-matched histologic samples, if available, using the automated Ventana PD-L1 SP263 assay. Paraffin 69-77 CD274 molecule Homo sapiens 31-36 33623414-5 2021 Microarray paraffin blocks from 70 samples of ADC (N=33), SqCC (N=24), and LCC (N=13) were used to create PD-L1 multiplex immunofluorescence assays with a Cell Signaling E1L3N clone. Paraffin 11-19 CD274 molecule Homo sapiens 106-111 33155793-13 2021 Therewithal, these findings also highlight the importance of taking into consideration of histological patterns, when choosing a paraffin block for the PDL1. Paraffin 129-137 CD274 molecule Homo sapiens 152-156 32333728-12 2020 Indeed, delays in tissue processing and paraffin embedding may lead to sub-optimal performance of PD-L1 staining on CBs. Paraffin 40-48 CD274 molecule Homo sapiens 98-103 33683684-6 2021 Here we describe an automated immunohistochemistry staining procedure that can be applied for the validation of multiple anti-PD-L1 antibody clones when used for the staining of formalin-fixed, paraffin-embedded lung cancer tissue sections. Paraffin 194-202 CD274 molecule Homo sapiens 126-131 33683685-5 2021 PD-L1 is a major immune checkpoint protein that mediates antitumor immune suppression and response, which is routinely detected using IHC in formalin-fixed and paraffin-embedded tissues as part of cancer clinical diagnostic workflows. Paraffin 160-168 CD274 molecule Homo sapiens 0-5 33299472-2 2020 PD-L1 testing has been validated and is currently certified only on formalin-fixed paraffin-embedded materials but not on cytological smears. Paraffin 83-91 CD274 molecule Homo sapiens 0-5 33299472-10 2020 With the same cut-off, and assuming as gold-standard the results on formalin-fixed paraffin-embedded materials, PD-L1 evaluation on smears showed specificity and negative predictive values of 98.1% and 93.9%, respectively. Paraffin 83-91 CD274 molecule Homo sapiens 112-117 33241207-3 2020 Immunohistochemical staining of PD-L1 (Clone 22C3) was applied to the sections obtained from paraffin blocks. Paraffin 93-101 CD274 molecule Homo sapiens 32-37 32079442-5 2020 PD-L1 expression was evaluated by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Paraffin 74-82 CD274 molecule Homo sapiens 0-5 31050216-2 2019 In particular, robust IHC assays performed on formalin-fixed, paraffin-embedded (FFPE) tumor tissue are widely used as surrogate markers for ALK and ROS1 rearrangements and for detecting programmed death ligand 1 (PD-L1) expression in patients with advanced NSCLC; in addition, they have become essential for treatment decisions. Paraffin 62-70 CD274 molecule Homo sapiens 214-219 31186735-5 2019 The expression of PD-L1 in 47 formalin-fixed, paraffin-embedded, NSCLC specimens was assessed using IHC and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Paraffin 46-54 CD274 molecule Homo sapiens 18-23 29862664-6 2019 Immunohistochemical staining of PD-L1 was performed on slides containing SC embedded in paraffin wax. Paraffin 88-100 CD274 molecule Homo sapiens 32-37 31987601-2 2020 MATERIALS AND METHODS: PD-L1 expression in paraffin-embedded tissues of ovarian cancer was detected by immunohistochemistry (IHC). Paraffin 43-51 CD274 molecule Homo sapiens 23-28 31605792-6 2020 RESULTS: PD-L1 staining was successful in 193 of 197 patient formalin-fixed paraffin-embedded blocks; of these, 128 of 193 were EGFRm-positive and 106 of 128 patients were randomized to treatment (osimertinib: 54; comparator: 52). Paraffin 76-84 CD274 molecule Homo sapiens 9-14 31535805-6 2019 In all cases PD-L1 expression in tumor cells was evaluated by immunohistochemical studies of paraffin block sections obtained under direct control of pathologist. Paraffin 93-101 CD274 molecule Homo sapiens 13-18 29135534-1 2019 Preliminary data suggest that tumor expression of programmed cell death ligand 1 (PD-L1) protein in human cancers, as determined by immunohistochemistry in formalin-fixed, paraffin-embedded tissue samples, may predict clinical response to anti-PD-1/PD-L1 therapy. Paraffin 172-180 CD274 molecule Homo sapiens 50-80 31030490-1 2019 Methods: Records of 132 women with EOC were reviewed.Immunostaining of PD-L1 was performed with formalin-fixed, paraffin-embedded specimens. Paraffin 112-120 CD274 molecule Homo sapiens 71-76 29135534-1 2019 Preliminary data suggest that tumor expression of programmed cell death ligand 1 (PD-L1) protein in human cancers, as determined by immunohistochemistry in formalin-fixed, paraffin-embedded tissue samples, may predict clinical response to anti-PD-1/PD-L1 therapy. Paraffin 172-180 CD274 molecule Homo sapiens 82-87 29135534-3 2019 The aim of the current study was to examine PD-L1 expression in formalin-fixed, paraffin-embedded malignant and nonmalignant cells from human tumors to establish potential characteristic patterns of PD-L1 expression in tumor tissues. Paraffin 80-88 CD274 molecule Homo sapiens 44-49 29938855-2 2018 PD-L1 IHC assays have been clinically validated only on formalin-fixed paraffin-embedded tissue; however, lung cancer is frequently diagnosed on cytology. Paraffin 71-79 CD274 molecule Homo sapiens 0-5 29084060-1 2019 Interpretative criteria for programmed death-ligand 1 (PD-L1) immunohistochemistry (IHC) have been largely based on data from formalin-fixed, paraffin-embedded tissues, despite the fact that cytologic specimens, especially cell blocks, are often the only or most readily available tissue for testing. Paraffin 142-150 CD274 molecule Homo sapiens 55-60 30121940-3 2018 We performed immunohistochemistry (IHC) for PD-L1 on formalin-fixed paraffin-embedded tissues. Paraffin 68-76 CD274 molecule Homo sapiens 44-49 28719380-2 2018 Preliminary data suggest that its detection using immunohistochemistry (IHC) in formalin-fixed and paraffin-embedded tissues may predict clinical response to PD-1/PD-L1 therapy. Paraffin 99-107 CD274 molecule Homo sapiens 163-168 30174892-8 2018 Expression of PD-L1 and PD-L2 on cytoplasm in paraffin embedded tumor samples were analyzed by immunohistochemistry staining and scored with a semi-quantitative method. Paraffin 46-54 CD274 molecule Homo sapiens 14-19 29767258-3 2018 PD-L1 mRNA expression of formalin-fixed paraffin-embedded paratumoral and intratumoral tissues was quantified by PCR. Paraffin 40-48 CD274 molecule Homo sapiens 0-5 29713584-2 2018 PD-L1 immunohistochemical testing is approved for formalin-fixed, paraffin-embedded (FFPE) surgical pathology specimens; however, the testing performance on FFPE cytology cell block specimens is unknown. Paraffin 66-74 CD274 molecule Homo sapiens 0-5 29491064-0 2018 Reduced Tumour Proportion Scores for Programmed Cell Death Ligand 1 in Stored Paraffin Tissue Sections. Paraffin 78-86 CD274 molecule Homo sapiens 37-67 30386718-2 2018 PD-L1 expression was evaluated by immunohistochemistry (IHC) using formalin-fixed paraffin-embedded (FFPE) specimens from 45 non-ccRCC patients with available tissue. Paraffin 82-90 CD274 molecule Homo sapiens 0-5 30323667-3 2018 Patients and methods: We investigated PD-L1 expression immunohistochemistry in formalin-fixed, paraffin-embedded tissues from 192 ICC patients undergoing curative resection and correlated our results with the clinicopathologic features and prognosis. Paraffin 95-103 CD274 molecule Homo sapiens 38-43 28716437-4 2017 METHODS: PD-L1 expression was analyzed by immunohistochemistry on paraffin-embedded tissue samples from 100 patients with cSCC. Paraffin 66-74 CD274 molecule Homo sapiens 9-14 29189265-4 2018 We report validation of PD-L1 IHC 28-8 pharmDx for PD-L1 detection on formalin-fixed, paraffin-embedded human melanoma specimens using Autostainer Link 48. Paraffin 86-94 CD274 molecule Homo sapiens 24-29 29189265-4 2018 We report validation of PD-L1 IHC 28-8 pharmDx for PD-L1 detection on formalin-fixed, paraffin-embedded human melanoma specimens using Autostainer Link 48. Paraffin 86-94 CD274 molecule Homo sapiens 51-56 28922567-7 2017 METHODS: PD-L1 expression was determined by a commercially available antibody (clone 28-8) in 74 formalin-fixed, paraffin-embedded cell blocks from body effusions obtained at diagnosis from patients with MM. Paraffin 113-121 CD274 molecule Homo sapiens 9-14 31043303-1 2018 INTRODUCTION: Evaluation of programmed cell death ligand-1 (PD-L1) on formalin-fixed paraffin-embedded (FFPE) histologic specimens is required for immunotherapy with pembrolizumab in non-small cell lung carcinoma (NSCLC). Paraffin 85-93 CD274 molecule Homo sapiens 28-58 31043303-1 2018 INTRODUCTION: Evaluation of programmed cell death ligand-1 (PD-L1) on formalin-fixed paraffin-embedded (FFPE) histologic specimens is required for immunotherapy with pembrolizumab in non-small cell lung carcinoma (NSCLC). Paraffin 85-93 CD274 molecule Homo sapiens 60-65 28978000-4 2017 PD-L1 was measured on formalin-fixed, paraffin-embedded tissue sections of RC specimens in all patients (n=61) and in 27 matched metastatic biopsy samples by immunohistochemistry. Paraffin 38-46 CD274 molecule Homo sapiens 0-5 27717372-2 2016 Here we describe a PD-L1 immunohistochemical (IHC) staining protocol developed by Ventana Medical Systems Inc. and key analytical parameters of its use in formalin-fixed, paraffin-embedded (FFPE) samples of non-small cell lung cancer (NSCLC) and head and neck squamous cell carcinoma (HNSCC). Paraffin 171-179 CD274 molecule Homo sapiens 19-24 28363612-4 2017 METHODS: Immunohistochemical staining of PD-L1 expression was performed in sections of 174 Formalin-fixed paraffin-embedded (FFPE) tissue blocks from surgery removed thyroid nodules. Paraffin 106-114 CD274 molecule Homo sapiens 41-46 27667773-5 2017 METHODS: Bristol-Myers Squibb and Dako previously reported on an automated PD-L1 immunohistochemical (IHC) assay that detects cell surface PD-L1 in formalin-fixed, paraffin-embedded, human tumor tissue specimens using Dako"s Autostainer Link 48. Paraffin 164-172 CD274 molecule Homo sapiens 75-80 27667773-5 2017 METHODS: Bristol-Myers Squibb and Dako previously reported on an automated PD-L1 immunohistochemical (IHC) assay that detects cell surface PD-L1 in formalin-fixed, paraffin-embedded, human tumor tissue specimens using Dako"s Autostainer Link 48. Paraffin 164-172 CD274 molecule Homo sapiens 139-144 27978870-3 2016 METHODS: The expressions of PD-1 and PD-L1 in 20 cases of PC paraffin-embedded tissue specimens were detected through immunohistochemistry. Paraffin 61-69 CD274 molecule Homo sapiens 37-42 27564404-2 2016 In the present study, we investigated the expression of the PD-1 ligand 1 (PD-L1) in a lymphoma microenvironment using paraffin-embedded tissue samples, and subsequently studied the detailed mechanism of upregulation of PD-L1 on macrophages using cultured human macrophages and lymphoma cell lines. Paraffin 119-127 CD274 molecule Homo sapiens 60-73 27564404-2 2016 In the present study, we investigated the expression of the PD-1 ligand 1 (PD-L1) in a lymphoma microenvironment using paraffin-embedded tissue samples, and subsequently studied the detailed mechanism of upregulation of PD-L1 on macrophages using cultured human macrophages and lymphoma cell lines. Paraffin 119-127 CD274 molecule Homo sapiens 75-80 26958083-4 2016 The expression of PD-L1 was evaluated using an anti-PD-L1 immunohistochemistry (IHC) antibody optimized for staining of formalin-fixed paraffin-embedded (FFPE) tissue samples. Paraffin 135-143 CD274 molecule Homo sapiens 18-23 27166394-4 2016 Copy number alterations of the PD-L1 gene in 11 cases were assessed in formalin-fixed, paraffin-embedded material using qRT-PCR. Paraffin 87-95 CD274 molecule Homo sapiens 31-36 27333219-2 2016 This automated IHC assay has been analytically verified and validated using Dako"s autostainer Link 48 and 22C3 mouse anti-PD-L1 monoclonal antibody to detect the PD-L1 expression in formalin-fixed paraffin-embedded human tumor tissue specimens. Paraffin 198-206 CD274 molecule Homo sapiens 163-168 27056074-3 2016 Here, we studied PD-L1 expression in paraffin-embedded samples from two cohorts of patients with cervical cancer: primary tumor samples from cohort I (squamous cell carcinoma, n=156 and adenocarcinoma, n=49) and primary and paired metastatic tumor samples from cohort II (squamous cell carcinoma, n=96 and adenocarcinoma, n=31). Paraffin 37-45 CD274 molecule Homo sapiens 17-22 24124529-5 2013 METHODS: Paraffin-embedded specimens from 143 eligible patients were used to investigate the expression of CD274 by immunohistochemistry. Paraffin 9-17 CD274 molecule Homo sapiens 107-112 26546452-7 2015 We demonstrate that 9A11, as well as two other cytoplasmic domain-specific mAbs, E1L3N and SP142, can clearly delineate the membrane of PD-L1-positive cells in formalin-fixed paraffin-embedded tissue and facilitate interpretation of staining results. Paraffin 175-183 CD274 molecule Homo sapiens 136-141 26317305-3 2015 We report an automated PD-L1 immunohistochemistry (IHC) assay, which was developed to detect cell surface PD-L1 in formalin-fixed paraffin-embedded human tumor tissue specimens using Dako"s Autostainer Link 48. Paraffin 130-138 CD274 molecule Homo sapiens 23-28 26317305-3 2015 We report an automated PD-L1 immunohistochemistry (IHC) assay, which was developed to detect cell surface PD-L1 in formalin-fixed paraffin-embedded human tumor tissue specimens using Dako"s Autostainer Link 48. Paraffin 130-138 CD274 molecule Homo sapiens 106-111 26037795-3 2015 METHODS: PD-L1 expression (cutoff 5%) was analyzed by immunohistochemistry with two different antibodies in BRAF(V600)-mutated formalin-fixed and paraffin-embedded samples from 80 consecutive MMP treated with BRAFi at a single institution. Paraffin 146-154 CD274 molecule Homo sapiens 9-14 19501877-5 2009 METHODS: Immunohistochemical methods were used to assess the tumor expression of B7-H1 and B7-H3 in paraffin-embedded tissues from 110 patients who had undergone partial or radical nephrectomy for renal AML and from 7 patients with LAM who had undergone lung biopsy. Paraffin 100-108 CD274 molecule Homo sapiens 81-96 23328703-0 2013 Immunohistochemical staining of B7-H1 (PD-L1) on paraffin-embedded slides of pancreatic adenocarcinoma tissue. Paraffin 49-57 CD274 molecule Homo sapiens 32-37 23328703-0 2013 Immunohistochemical staining of B7-H1 (PD-L1) on paraffin-embedded slides of pancreatic adenocarcinoma tissue. Paraffin 49-57 CD274 molecule Homo sapiens 39-44 23328703-9 2013 The anti-B7-H1 monoclonal antibody (clone 5H1) developed by Chen and coworkers has been shown to produce reliable staining results in cryosections of multiple types of human neoplastic tissues, but staining on paraffin-embedded slides had been a challenge until recently. Paraffin 210-218 CD274 molecule Homo sapiens 9-14 23328703-10 2013 We have developed the B7-H1 staining protocol for paraffin-embedded slides of pancreatic adenocarcinoma tissues. Paraffin 50-58 CD274 molecule Homo sapiens 22-27 18676751-3 2008 EXPERIMENTAL DESIGN: Immunohistochemistry for B7-H1, B7-H3, and PD-1 was done on paraffin-embedded sections from 318 consecutive patients with UCC who underwent radical cystectomy. Paraffin 81-89 CD274 molecule Homo sapiens 46-68 17360651-4 2007 Here we generated a new Ab against PD-1 ligand 1 (PD-L1) and analyzed the expression level of PD-Ls in human ovarian cancer using paraffin-embedded specimens. Paraffin 130-138 CD274 molecule Homo sapiens 35-48