PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15280438-4 2004 Rotenone also increased the amount of alpha-synuclein in cells showing morphological changes in response to rotenone. Rotenone 0-8 synuclein alpha Homo sapiens 38-53 15280438-4 2004 Rotenone also increased the amount of alpha-synuclein in cells showing morphological changes in response to rotenone. Rotenone 108-116 synuclein alpha Homo sapiens 38-53 15280438-6 2004 Whereas a decreased Bad amount bound to 14-3-3 proteins, rotenone increased alpha-synuclein binding to these proteins. Rotenone 57-65 synuclein alpha Homo sapiens 76-91 14672949-4 2004 Here we report that a combination of 1-methyl-4-phenylpyridinium ion (MPP(+)) or rotenone and proteasome inhibition causes the appearance of alpha-synuclein-positive inclusion bodies. Rotenone 81-89 synuclein alpha Homo sapiens 141-156 15234983-1 2004 To identify the proteins associated with soluble alpha-synuclein (AS) that might promote AS aggregation, a key event leading to neurodegeneration, we quantitatively compared protein profiles of AS-associated protein complexes in MES cells exposed to rotenone, a pesticide that produces parkinsonism in animals and induces Lewy body (LB)-like inclusions in the remaining dopaminergic neurons, and to vehicle. Rotenone 250-258 synuclein alpha Homo sapiens 49-64 15234983-1 2004 To identify the proteins associated with soluble alpha-synuclein (AS) that might promote AS aggregation, a key event leading to neurodegeneration, we quantitatively compared protein profiles of AS-associated protein complexes in MES cells exposed to rotenone, a pesticide that produces parkinsonism in animals and induces Lewy body (LB)-like inclusions in the remaining dopaminergic neurons, and to vehicle. Rotenone 250-258 synuclein alpha Homo sapiens 66-68 15234983-2 2004 We identified more than 250 proteins associated with Nonidet P-40 soluble AS, and demonstrated that at least 51 of these proteins displayed significant differences in their relative abundance in AS complexes under conditions where rotenone was cytotoxic and induced formation of cytoplasmic inclusions immunoreactive to anti-AS. Rotenone 231-239 synuclein alpha Homo sapiens 74-76 15234983-2 2004 We identified more than 250 proteins associated with Nonidet P-40 soluble AS, and demonstrated that at least 51 of these proteins displayed significant differences in their relative abundance in AS complexes under conditions where rotenone was cytotoxic and induced formation of cytoplasmic inclusions immunoreactive to anti-AS. Rotenone 231-239 synuclein alpha Homo sapiens 195-197 14550906-0 2003 Alpha-synuclein expression in HEK293 cells enhances the mitochondrial sensitivity to rotenone. Rotenone 85-93 synuclein alpha Homo sapiens 0-15 14550906-4 2003 However, both wild-type and mutant G209A alpha-synuclein expression enhanced the fall in mitochondrial membrane potential induced by the complex I inhibitor rotenone. Rotenone 157-165 synuclein alpha Homo sapiens 41-56 12548372-2 2002 From an experimental point of view, a new model of parkinsonism induced by rotenone, a diffuse insecticide, has been proposed, and in vitro studies have provided proof that several pesticides stimulate the formation of alpha-synuclein fibrils (one of the principal constituents of Lewy bodies). Rotenone 75-83 synuclein alpha Homo sapiens 219-234 12807439-6 2003 Similarly, alpha-synuclein protected cells from the complex I inhibitor rotenone and 3-nitroproprionic acid, a complex II inhibitor. Rotenone 72-80 synuclein alpha Homo sapiens 11-26 12177198-4 2002 Short-term rotenone treatment (1 week) elevated soluble alpha-synuclein protein levels without changing message levels, suggesting that alpha-synuclein degradation was retarded. Rotenone 11-19 synuclein alpha Homo sapiens 56-71 12177198-4 2002 Short-term rotenone treatment (1 week) elevated soluble alpha-synuclein protein levels without changing message levels, suggesting that alpha-synuclein degradation was retarded. Rotenone 11-19 synuclein alpha Homo sapiens 136-151 12177198-5 2002 Chronic rotenone exposure (4 weeks) increased levels of SDS-insoluble alpha-synuclein and ubiquitin. Rotenone 8-16 synuclein alpha Homo sapiens 70-85 12177198-9 2002 These studies indicate that chronic low-grade complex I inhibition caused by rotenone exposure induces accumulation and aggregation of alpha-synuclein and ubiquitin, progressive oxidative damage, and caspase-dependent death, mechanisms that may be central to PD pathogenesis. Rotenone 77-85 synuclein alpha Homo sapiens 135-150 12151787-4 2002 We demonstrate that expression of all alpha-synuclein isoforms enhances toxicity of general complex I inhibition (rotenone), but only the expression of mutant alpha-synucleins induces significant increased DAT-dependent toxicity of very low concentrations of MPP+ compared to wild-type protein. Rotenone 114-122 synuclein alpha Homo sapiens 38-53 11679584-7 2002 In our recent study, we showed that mitochondrial inhibitors such as rotenone, induced alpha-synuclein aggregation in cells. Rotenone 69-77 synuclein alpha Homo sapiens 87-102 11724769-4 2002 Treatment with rotenone, an inhibitor of complex I, resulted in an increase of detergent-resistant alpha-synuclein aggregates and a reduction in ATP level. Rotenone 15-23 synuclein alpha Homo sapiens 99-114 11679584-8 2002 In the present study using rotenone-treated cells, the earliest appearance of alpha-synuclein oligomeric species was observed in membranous compartments. Rotenone 27-35 synuclein alpha Homo sapiens 78-93 35457010-1 2022 Rotenone (ROT) inhibits mitochondrial complex I, leading to reactive oxygen species formation, which causes neurodegeneration and alpha-synuclein (alpha-syn) aggregation and, consequently, Parkinson"s disease. Rotenone 0-8 synuclein alpha Homo sapiens 130-145 34510532-5 2021 We observed that alpha-syn potentiates the mitochondrial membrane potential (Deltapsim ) loss induced by rotenone, increases mitophagy and mitochondrial Ca2+ overload. Rotenone 105-113 synuclein alpha Homo sapiens 17-26 34306319-9 2021 Rotenone-induced LRRK2 upregulation impaired the clearance of alpha-syn fibrils. Rotenone 0-8 synuclein alpha Homo sapiens 62-71 34306319-10 2021 Treatment with LRRK2 inhibitor mitigated rotenone-induced cellular senescence and alpha-syn accumulation. Rotenone 41-49 synuclein alpha Homo sapiens 82-91 34306319-11 2021 Conclusions: Rotenone-induced upregulation of LRRK2 kinase activity promoted cellular senescence, which enhanced alpha-syn accumulation. Rotenone 13-21 synuclein alpha Homo sapiens 113-122 11445065-3 2001 Here we show that several pesticides, including rotenone, dieldrin and paraquat, induce a conformational change in alpha-synuclein and significantly accelerate the rate of formation of alpha-synuclein fibrils in vitro. Rotenone 48-56 synuclein alpha Homo sapiens 115-130 11445065-3 2001 Here we show that several pesticides, including rotenone, dieldrin and paraquat, induce a conformational change in alpha-synuclein and significantly accelerate the rate of formation of alpha-synuclein fibrils in vitro. Rotenone 48-56 synuclein alpha Homo sapiens 185-200 35471724-0 2022 Correction to: Intranasal Exposure to Low-Dose Rotenone Induced Alpha-Synuclein Accumulation and Parkinson"s Like Symptoms Without Loss of Dopaminergic Neurons. Rotenone 47-55 synuclein alpha Homo sapiens 64-79 35457010-1 2022 Rotenone (ROT) inhibits mitochondrial complex I, leading to reactive oxygen species formation, which causes neurodegeneration and alpha-synuclein (alpha-syn) aggregation and, consequently, Parkinson"s disease. Rotenone 0-8 synuclein alpha Homo sapiens 147-156 35457010-1 2022 Rotenone (ROT) inhibits mitochondrial complex I, leading to reactive oxygen species formation, which causes neurodegeneration and alpha-synuclein (alpha-syn) aggregation and, consequently, Parkinson"s disease. Rotenone 10-13 synuclein alpha Homo sapiens 130-145 35457010-1 2022 Rotenone (ROT) inhibits mitochondrial complex I, leading to reactive oxygen species formation, which causes neurodegeneration and alpha-synuclein (alpha-syn) aggregation and, consequently, Parkinson"s disease. Rotenone 10-13 synuclein alpha Homo sapiens 147-156 35277478-6 2022 SD and SDA also lower rotenone-induced-alpha-synuclein (alpha-syn) expression in human SH-SY5Y cells, and alpha-syn oligomerization in alpha-syn-overexpressing-HEK293 cells. Rotenone 22-30 synuclein alpha Homo sapiens 56-65 32949572-9 2020 We further delineate, that the senescent secretome exaggerates rotenone-induced neurodegeneration in midbrain neurons differentiated from human induced pluripotent stem cells (hiPSC) of PD patients with alpha-synuclein gene (SNCA) locus duplication. Rotenone 63-71 synuclein alpha Homo sapiens 203-218 33961406-4 2021 Additionally, rotenone induces in vivo aggregation of alpha-synuclein, the major component of Lewy bodies and Lewy neurites found in the brain of PD patients and another hallmark of PD neuropathology. Rotenone 14-22 synuclein alpha Homo sapiens 54-69 33961406-5 2021 Some in vivo rotenone models also reproduce peripheral signs of PD, such as reduced intestinal motility and peripheral alpha-synuclein aggregation, both of which are thought to precede classical signs of PD in humans, such as cogwheel rigidity, bradykinesia, and resting tremor. Rotenone 13-21 synuclein alpha Homo sapiens 119-134 35023548-5 2022 Multiple forms of alpha-synuclein, including monomer, low and high molecular weight oligomers, were differentially present in the 2D and 3D cells, but mostly remained unchanged upon the MPP+ or rotenone treatment. Rotenone 194-202 synuclein alpha Homo sapiens 18-33 34984583-9 2022 Besides, suppression of the CIP2A expression alleviates rotenone induced aggregation of the alpha-syn as well as phosphorylation of the alpha-syn in SH-SY5Y cells, which is associated with increased PP2A activity. Rotenone 56-64 synuclein alpha Homo sapiens 92-101 34984583-9 2022 Besides, suppression of the CIP2A expression alleviates rotenone induced aggregation of the alpha-syn as well as phosphorylation of the alpha-syn in SH-SY5Y cells, which is associated with increased PP2A activity. Rotenone 56-64 synuclein alpha Homo sapiens 136-145 32086435-6 2021 In contrast, depletion of C/EBPbeta from human alpha-Syn Tg mice abolishes rotenone-elicited PD pathologies and motor impairments via downregulating the expression of these key factors. Rotenone 75-83 synuclein alpha Homo sapiens 47-56 33552161-0 2021 Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis. Rotenone 107-115 synuclein alpha Homo sapiens 124-139 33469419-5 2020 Our results showed that rotenone could induce the up-regulation of alpha-synuclein (alpha-syn) in both mRNA and protein levels. Rotenone 24-32 synuclein alpha Homo sapiens 67-82 33469419-5 2020 Our results showed that rotenone could induce the up-regulation of alpha-synuclein (alpha-syn) in both mRNA and protein levels. Rotenone 24-32 synuclein alpha Homo sapiens 67-76 33469419-7 2020 Further study showed that high expression of Ndfip1 could protect SH-SY5Y cells against rotenone-induced neurotoxicity and antagonize the rotenone-induced increase in alpha-syn protein levels. Rotenone 138-146 synuclein alpha Homo sapiens 167-176 33469419-10 2020 Our findings provide specific identification of Ndfip1 proteins to inhibit the increase of alpha-syn in rotenone-induced SH-SY5Y cells. Rotenone 104-112 synuclein alpha Homo sapiens 91-100 32949572-9 2020 We further delineate, that the senescent secretome exaggerates rotenone-induced neurodegeneration in midbrain neurons differentiated from human induced pluripotent stem cells (hiPSC) of PD patients with alpha-synuclein gene (SNCA) locus duplication. Rotenone 63-71 synuclein alpha Homo sapiens 225-229 32488468-1 2020 The detrimental impact on the food chain due to the overuse of rotenone is partly responsible for alpha-synuclein (alpha-syn) mediated neurotoxicity. Rotenone 63-71 synuclein alpha Homo sapiens 98-113 32905883-5 2020 Live intravital imaging of the intact zebrafish CNS at cellular resolution showed unequivocally that alpha-Synuclein amplified dynamic cytoplasmic peroxide flux in dopaminergic neurons following exposure to the mitochondrial complex I inhibitors MPP+ or rotenone. Rotenone 254-262 synuclein alpha Homo sapiens 101-116 33163674-13 2020 The expression of alpha-synuclein in the substantia nigra increased in the rotenone group but decreased in the sesaminol group. Rotenone 75-83 synuclein alpha Homo sapiens 18-33 33110167-3 2020 Here, to understand the mechanism of misfolding and aggregation initiation, we induced alpha-syn aggregation with rotenone, an established chemical inducer of PD like symptoms. Rotenone 114-122 synuclein alpha Homo sapiens 87-96 33177988-5 2020 This study investigated the clearance effects of hepcidin on alpha-syn induced by a relatively low concentration of rotenone exposure or alpha-syn overexpression to elucidate the potential clearance pathway involved in this process. Rotenone 116-124 synuclein alpha Homo sapiens 61-70 33177988-7 2020 alpha-syn expression and iron content increased under a low concentration rotenone (25 nM for 3 days) treatment in SH-SY5Y cells. Rotenone 74-82 synuclein alpha Homo sapiens 0-9 33177988-10 2020 Hepcidin also played a role in reducing alpha-syn accumulation in rotenone and alpha-syn overexpression conditions. Rotenone 66-74 synuclein alpha Homo sapiens 40-49 33177988-12 2020 In conclusion, hepcidin eliminated alpha-syn expression via the autophagy pathway in rotenone-treated and alpha-syn overexpression SH-SY5Y cells. Rotenone 85-93 synuclein alpha Homo sapiens 35-44 32488468-1 2020 The detrimental impact on the food chain due to the overuse of rotenone is partly responsible for alpha-synuclein (alpha-syn) mediated neurotoxicity. Rotenone 63-71 synuclein alpha Homo sapiens 98-107 32444560-7 2020 RESULTS: We validated our ELISA using rotenone-induced alterations of alpha-syn levels in human dopaminergic neurons. Rotenone 38-46 synuclein alpha Homo sapiens 70-79 32624505-0 2020 Death-associated Protein Kinase 1 Phosphorylates alpha-Synuclein at Ser129 and Exacerbates Rotenone-induced Toxic Aggregation of alpha-Synuclein in Dopaminergic SH-SY5Y Cells. Rotenone 91-99 synuclein alpha Homo sapiens 129-144 32624505-10 2020 We also showed that DAPK1 enhances rotenone-induced aggregation of alpha-synuclein, potentiating neuronal cell death. Rotenone 35-43 synuclein alpha Homo sapiens 67-82 32071790-8 2020 Cytotoxicity of aSyn overexpression was indirectly revealed by the addition of pro-oxidant rotenone. Rotenone 91-99 synuclein alpha Homo sapiens 16-20 32071790-9 2020 Pretreatment with partially reduced graphene oxide, an apoptotic agent, increased toxicity of rotenone in low-aSyn neurons, but, it did not in high-aSyn neurons. Rotenone 94-102 synuclein alpha Homo sapiens 110-114 31778768-4 2020 Alpha mangostin is an important polyphenolic xanthone reported for its neuroprotective effect against rotenone-induced alpha-synuclein aggregation and loss of tyrosine hydroxylase positive (TH+)-neurons in SHSY-5Y cells. Rotenone 102-110 synuclein alpha Homo sapiens 119-134 31649329-5 2020 Chronic oral administration of the neurotoxin rotenone induces AEP activation and alpha-Syn N103/Tau N368 complex formation in the gut, eliciting constipation and dopaminergic neuronal death in an AEP-dependent manner. Rotenone 46-54 synuclein alpha Homo sapiens 82-91 30907971-2 2019 The mitochondrial complex I inhibitor, rotenone, is selectively toxic to dopaminergic neurons, and reproduces several neuropathological features of Parkinson"s disease, including alpha-synuclein pathology. Rotenone 39-47 synuclein alpha Homo sapiens 179-194 30904435-4 2019 Under the exposure to rotenone or not, the expression of constitutive proteasome beta subunits, i-proteasome betai subunits, antigen presentation related proteins, alpha-syn and TH were detected by Western blot in PSMB9-silenced or -overexpressed cells, and the proteasomal activities were detected by fluorogenic peptide substrates. Rotenone 22-30 synuclein alpha Homo sapiens 164-173 31611767-0 2019 The Impairments of alpha-Synuclein and Mechanistic Target of Rapamycin in Rotenone-Induced SH-SY5Y Cells and Mice Model of Parkinson"s Disease. Rotenone 74-82 synuclein alpha Homo sapiens 19-34 31611767-9 2019 Western blotting revealed that ROT inhibited TH and Parkin expression while increasing alpha-syn and PINK1 expression in both SH-SY5Y cells and injected mice, consistent with disruption of mitochondrial function. Rotenone 31-34 synuclein alpha Homo sapiens 87-96 30692508-2 2019 Electrophysiological recordings of field postsynaptic potentials (fEPSPs) and Ca2+ measures in striatal brain slices and differentiated SH-SY5Y cells showed that co-application of alpha-syn and the neurotoxic pesticide rotenone (Rot) induced Ca2+ dysregulation and alteration of both synaptic transmission and cell function. Rotenone 219-227 synuclein alpha Homo sapiens 180-189 30692508-2 2019 Electrophysiological recordings of field postsynaptic potentials (fEPSPs) and Ca2+ measures in striatal brain slices and differentiated SH-SY5Y cells showed that co-application of alpha-syn and the neurotoxic pesticide rotenone (Rot) induced Ca2+ dysregulation and alteration of both synaptic transmission and cell function. Rotenone 229-232 synuclein alpha Homo sapiens 180-189 30141137-6 2018 In addition, we assessed the involvement of GE on rotenone-induced dysfunction in autophagy machinery via alpha-synuclein accumulation induced ER stress. Rotenone 50-58 synuclein alpha Homo sapiens 106-121 28573674-5 2018 When these cells were treated with rotenone, there was a loss of mitochondrial membrane potential, especially in cells expressing mutant alpha-synuclein. Rotenone 35-43 synuclein alpha Homo sapiens 137-152 30081046-0 2018 P75 Involved in the Ubiquitination of alpha-synuclein in Rotenone-based Parkinson"s Disease Models. Rotenone 57-65 synuclein alpha Homo sapiens 38-53 30081046-7 2018 In cellular models of rotenone-mediated neurotoxicity, the interactions between p75 and siAH were revealed by immunoprecipitation; the colocalization of p75 with alpha-syn was observed in the cytoplasm; p75 promoted nuclear expression of NF-kappaB (p65), which might interact with the promoter of the siAH gene. Rotenone 22-30 synuclein alpha Homo sapiens 162-171 30081046-8 2018 Moreover, siRNA-mediated p75 depletion reduced the upregulation of alpha-syn and nuclear expression of p65 and protected against cell apoptosis induced by rotenone. Rotenone 155-163 synuclein alpha Homo sapiens 67-76 28696167-0 2017 Neuroprotective effect of alpha-mangostin on mitochondrial dysfunction and alpha-synuclein aggregation in rotenone-induced model of Parkinson"s disease in differentiated SH-SY5Y cells. Rotenone 106-114 synuclein alpha Homo sapiens 75-90 28696167-3 2017 The rotenone-induced aggregation of alpha-synuclein and loss of TH were alleviated by alpha-mangostin. Rotenone 4-12 synuclein alpha Homo sapiens 36-51 28236214-9 2017 Mutant Atx3 and alpha-Syn also potentiated altered redox status induced by iron and rotenone, a hint to how these proteins might influence neuronal dysfunction under pro-oxidant conditions. Rotenone 84-92 synuclein alpha Homo sapiens 16-25 28417442-6 2017 The most common toxicants used to model PD including rotenone, paraquat, and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine have been shown to interact with many of the genes linked with PD such as alpha-synuclein. Rotenone 53-61 synuclein alpha Homo sapiens 196-211 27997067-0 2017 Rotenone and elevated extracellular potassium concentration induce cell-specific fibrillation of alpha-synuclein in axons of cholinergic enteric neurons in the guinea-pig ileum. Rotenone 0-8 synuclein alpha Homo sapiens 97-112 27997067-7 2017 METHODS: In this study, we used immunohistochemistry to detect alpha-synuclein oligomers and fibrils via conformation-specific antibodies after rotenone treatment or prolonged exposure to high [K+ ] in ex vivo segments of guinea-pig ileum maintained in organotypic culture. Rotenone 144-152 synuclein alpha Homo sapiens 63-78 27997067-8 2017 KEY RESULTS: Rotenone and prolonged raising of [K+ ] caused accumulation of alpha-synuclein fibrils in the axons of cholinergic enteric neurons. Rotenone 13-21 synuclein alpha Homo sapiens 76-91 28164279-7 2017 Calcipotriol significantly decreased the frequency of alpha-synuclein aggregate positive cells subjected to treatments that cause raised intracellular-free Ca(II) (potassium depolarization, KCl/H2 O2 combined treatment, and rotenone) in a dose-dependent manner and increased viability. Rotenone 224-232 synuclein alpha Homo sapiens 54-69 28137648-7 2017 Rotenone treatment of SH-SY5Y cells reduced their viability and alpha-synuclein expression; simultaneous exposure to rosuvastatin significantly restored these parameters. Rotenone 0-8 synuclein alpha Homo sapiens 64-79 28197072-4 2017 Here in this study, we investigated the interaction between HMGB1 and alpha-synuclein in rotenone-induced PD cell models and their roles in autophagy flux. Rotenone 89-97 synuclein alpha Homo sapiens 70-85 28197072-8 2017 Based on these findings, we propose that HMGB1 is involved in rotenone-induced dopaminergic cell death via interacting with alpha-synuclein, perturbing the autophagy process, aggravating protein aggregation and finally propelling dopaminergic neurons to move from morbidity to mortality. Rotenone 62-70 synuclein alpha Homo sapiens 124-139 27053302-11 2016 SIRT3 knockdown significantly worsen rotenone-induced decline of cell viability (p < 0.01) and enhanced cell apoptosis (p < 0.01), exacerbated the decrease of SOD (p < 0.05) and GSH (p < 0.05), and augmented the accumulation of alpha-synuclein (p < 0.05). Rotenone 37-45 synuclein alpha Homo sapiens 240-255 26156288-9 2016 Rotenone treatment of SH-SY5Y cells reduced their viability, increased reactive oxygen species levels, and induced apoptosis and alpha-synuclein expression, and simultaneous exposure to EPO significantly reduced these effects. Rotenone 0-8 synuclein alpha Homo sapiens 129-144 27053302-12 2016 While SIRT3 overexpression dramatically increased cell viability (p < 0.01), and decreased cell apoptosis (p < 0.01), prevented the accumulation of alpha-synuclein (p < 0.05), suppressed the reducing of SOD (p < 0.05) and GSH (p < 0.01), decreased ROS generation (p < 0.05), and alleviated MMP collapse (p < 0.01) induced by rotenone. Rotenone 346-354 synuclein alpha Homo sapiens 154-169 27178445-6 2016 RESULTS: we found that rotenone, a widely used mitochondrial Complex I inhibitor decreases the density of sympathetic neurites innervating the gut in vivo, while in vitro, it induces the redistribution of intracellular alpha-synuclein and neurite degeneration. Rotenone 23-31 synuclein alpha Homo sapiens 219-234 27012437-0 2016 The novel mechanism of rotenone-induced alpha-synuclein phosphorylation via reduced protein phosphatase 2A activity. Rotenone 23-31 synuclein alpha Homo sapiens 40-55 27012437-3 2016 However, the mechanisms whereby rotenone regulates alpha-syn phosphorylation remain unknown. Rotenone 32-40 synuclein alpha Homo sapiens 51-60 27012437-11 2016 C2 ceramide and SKI606 significantly reversed the rotenone-induced phosphorylation and aggregation of alpha-syn by increasing PP2A activity. Rotenone 50-58 synuclein alpha Homo sapiens 102-111 27012437-12 2016 These results demonstrate that rotenone-reduced PP2A activity via Src kinase is involved in the phosphorylation of alpha-syn. Rotenone 31-39 synuclein alpha Homo sapiens 115-124 27133439-5 2016 Considering that the mitochondrial complex I inhibitor rotenone, used to generate PD animal models, induces alpha-synuclein aggregation, this study was designed in order to verify whether rotenone exposure leads to hsc70 alteration possibly contributing to alpha-synuclein aggregation. Rotenone 55-63 synuclein alpha Homo sapiens 108-123 26826319-9 2016 Furthermore rotenone induced PD like features were characterized by decrease in tyrosine hydroxylase expression and increase in alpha-synuclein expression. Rotenone 12-20 synuclein alpha Homo sapiens 128-143 27133439-10 2016 We also observed that the toxic effect of rotenone on alpha-synuclein levels was amplified when macroautophagy was inhibited, although rotenone-induced hsc70 reduction was independent from macroautophagy. Rotenone 42-50 synuclein alpha Homo sapiens 54-69 27133439-5 2016 Considering that the mitochondrial complex I inhibitor rotenone, used to generate PD animal models, induces alpha-synuclein aggregation, this study was designed in order to verify whether rotenone exposure leads to hsc70 alteration possibly contributing to alpha-synuclein aggregation. Rotenone 188-196 synuclein alpha Homo sapiens 257-272 26363201-6 2015 While rotenone-treated mature VM neurons showed alpha-synuclein aggregation and sensitivity to DA neurons, immature VM neurons exhibited only DA neuronal sensitivity but not alpha-synuclein aggregation. Rotenone 6-14 synuclein alpha Homo sapiens 48-63 25497491-6 2014 Such alpha-syn aggregation is induced in only a few animal models, for example perikaryon inclusions are found in rats administered rotenone, aggregates with a neuritic morphology develop in mice overexpressing either mutated or wild-type alpha-syn, and in Smad3 deficient mice, aggregates form extensively in the perikaryon and neurites of specific brain nuclei. Rotenone 132-140 synuclein alpha Homo sapiens 5-14 26378986-4 2015 The antinausea drugs, cinnarizine and metoclopramide, do not bind to alpha-synuclein, whereas amphetamine and the herbicides, paraquat and rotenone, bind tightly and cause alpha-synuclein to adopt a more compact conformation. Rotenone 139-147 synuclein alpha Homo sapiens 172-187 26385697-11 2015 Finally, we reported that Fer-1 mitigated rotenone-induced alpha-syn aggregation. Rotenone 42-50 synuclein alpha Homo sapiens 59-68 25846226-0 2015 Alpha-synuclein modulates NR2B-containing NMDA receptors and decreases their levels after rotenone exposure. Rotenone 90-98 synuclein alpha Homo sapiens 0-15 25846226-7 2015 Moreover, we found that absence of alpha-syn abolishes the rotenone-dependent decrease of NR2B levels and reduces neuronal vulnerability in primary cortical neurons. Rotenone 59-67 synuclein alpha Homo sapiens 35-44 25447323-0 2015 Unveiling the role of the pesticides paraquat and rotenone on alpha-synuclein fibrillation in vitro. Rotenone 50-58 synuclein alpha Homo sapiens 62-77 25665531-7 2015 Our data revealed that A53T alpha-syn could disrupt mitochondrial dynamics and increase the neuronal susceptibility to neurotoxin rotenone. Rotenone 130-138 synuclein alpha Homo sapiens 28-37 24970188-4 2013 The thioflavine T (ThT) fluorescence assay studies provide evidence that early-phase misfolded alpha-synuclein forms are affected by rotenone and that the fibrillation process is accelerated. Rotenone 133-141 synuclein alpha Homo sapiens 95-110 25240592-0 2014 Inhibition of prolyl oligopeptidase increases the survival of alpha-synuclein overexpressing cells after rotenone exposure by reducing alpha-synuclein oligomers. Rotenone 105-113 synuclein alpha Homo sapiens 62-77 25109238-6 2014 Exposure to FeSO4 or rotenone enhanced intracellular ROS levels, including superoxide anions, in both types of cells, along with alpha-syn Ser129 phosphorylation and mitochondrial depolarization. Rotenone 21-29 synuclein alpha Homo sapiens 129-138 24970188-7 2013 We believe that these data provide useful information for a better understanding of the molecular basis of rotenone-induced misfolding and aggregation of alpha-synuclein. Rotenone 107-115 synuclein alpha Homo sapiens 154-169 23259041-1 2012 Rotenone, which selectively inhibits mitochondrial complex I, induces oxidative stress, alpha-synuclein accumulation, and dopaminergic neuron death, principal pathological features of Parkinson"s disease. Rotenone 0-8 synuclein alpha Homo sapiens 88-103 23743292-8 2013 Environmental factors, such as MPTP and rotenone, have been reported to induce selective degeneration of the nigrostriatal pathways leading to alpha-syn-positive inclusions, possibly by inhibiting mitochondrial complex I of the respiratory chain and subsequently increasing oxidative stress. Rotenone 40-48 synuclein alpha Homo sapiens 143-152 23535362-3 2013 Our objective was to precisely assess changes in alpha-syn levels in human neuroblastoma (SH-SY5Y) and melanoma (SK-MEL-2) cell lines following acute exposure to pesticides (rotenone, paraquat, maneb, and glyphosate) using Western blot and flow cytometry. Rotenone 174-182 synuclein alpha Homo sapiens 49-58 23535362-5 2013 We found that endogenous alpha-syn levels in the SH-SY5Y neuroblastoma cell line were markedly increased by paraquat, and to a lesser extent by rotenone and maneb, but not by glyphosate. Rotenone 144-152 synuclein alpha Homo sapiens 25-34 23535362-6 2013 Rotenone also clearly increased endogenous alpha-syn levels in the SK-MEL-2 melanoma cell line. Rotenone 0-8 synuclein alpha Homo sapiens 43-52 23544381-3 2013 The currently used pesticides such as rotenone and paraquat could disrupt mitochondrial bioenergetic function, reactive oxygen metabolism, redox function and promote alpha-synuclein aggregation. Rotenone 38-46 synuclein alpha Homo sapiens 166-181 23153578-7 2013 Further, when these animals were exposed to rotenone, a mitochondrial toxin linked to PD, they showed heightened sensitivity, indicating that alpha-synuclein expression modulates the vulnerability to mitochondrial impairment. Rotenone 44-52 synuclein alpha Homo sapiens 142-157 23984410-0 2013 Rotenone upregulates alpha-synuclein and myocyte enhancer factor 2D independently from lysosomal degradation inhibition. Rotenone 0-8 synuclein alpha Homo sapiens 21-36 23984410-3 2013 Rotenone induced an upregulation of alpha-synuclein and MEF2D protein levels through the stimulation of their de novo synthesis rather than through a reduction of their CMA-mediated degradation. Rotenone 0-8 synuclein alpha Homo sapiens 36-51 23984410-7 2013 These results highlight that rotenone affects alpha-synuclein and MEF2D protein levels through a mechanism independent from lysosomal degradation inhibition. Rotenone 29-37 synuclein alpha Homo sapiens 46-61 22319455-6 2012 Similarly, human TRAP1 overexpression in rat primary cortical neurons rescued [A53T]alpha-Synuclein-induced sensitivity to rotenone treatment. Rotenone 123-131 synuclein alpha Homo sapiens 84-99 22967820-8 2012 Environmental factors, such as MPTP and rotenone, have been reported to induce selective degeneration of the nigrostriatal pathways leading to alpha-syn-positive inclusions, possibly by inhibiting mitochondrial complex I of the respiratory chain and subsequently increasing oxidative stress. Rotenone 40-48 synuclein alpha Homo sapiens 143-152 21238542-0 2011 Over-expression of alpha-synuclein 98 triggers intracellular oxidative stress and enhances susceptibility to rotenone. Rotenone 109-117 synuclein alpha Homo sapiens 19-34 21658409-0 2011 Neuroprotection of alpha-synuclein under acute and chronic rotenone and maneb treatment is abolished by its familial Parkinson"s disease mutations A30P, A53T and E46K. Rotenone 59-67 synuclein alpha Homo sapiens 19-34 21658409-5 2011 Compared with untransfected SH-SY5Y cells, wild type alpha-Syn attenuated rotenone and maneb-induced cell death along with an attenuation of toxin-induced mitochondrial membrane potential changes and Reactive Oxygen Species level, whereas the mutant alpha-Syn constructs exacerbated environmental toxins-induced cytotoxicity. Rotenone 74-82 synuclein alpha Homo sapiens 53-62 21419806-2 2011 Rotenone in rodent models reproduces key features of human PD by impairing the mitochondrial electron transport chain, leading to intracellular alpha-synuclein aggregates and functional impairments typical for PD. Rotenone 0-8 synuclein alpha Homo sapiens 144-159 21085094-0 2010 Oral administration of rotenone using a gavage and image analysis of alpha-synuclein inclusions in the enteric nervous system. Rotenone 23-31 synuclein alpha Homo sapiens 69-84 20705047-2 2010 Rotenone administration in animals induces neurodegeneration accompanied by alpha-synuclein-positive Lewy body-like inclusions, recapturing typical histopathological features of PD. Rotenone 0-8 synuclein alpha Homo sapiens 76-91 19857570-3 2010 Using parkinsonism mimetics (MPP(+), rotenone) and related oxidants, we have identified an oxidant-induced alternative splicing of alpha-syn mRNA, generating a shorter isoform of alpha-syn with deleted exon-5 (112-syn). Rotenone 37-45 synuclein alpha Homo sapiens 131-140 20596942-0 2010 Dual effects of alpha-synuclein on neurotoxicity induced by low dosage of rotenone are dependent on exposure time in dopaminergic neuroblastoma cells. Rotenone 74-82 synuclein alpha Homo sapiens 16-31 20596942-1 2010 This study was designed to investigate the effects of alpha-synuclein on toxicity induced by long-term exposure to relatively low concentrations of rotenone. Rotenone 148-156 synuclein alpha Homo sapiens 54-69 20596942-2 2010 Compared with the control groups, the inhibition of cell viability which overexpressed alpha-synuclein (SH-SY5Y-Syn) improved after 1 and 2 weeks of rotenone treatment. Rotenone 149-157 synuclein alpha Homo sapiens 87-102 20596942-3 2010 The complex I activity was greater and the mitochondrial membrane swelling intensity was reduced after 1 and 2 weeks of treatment, which indicated that alpha-synuclein, at least in part, resists the rotenone-induced oxidative stress. Rotenone 199-207 synuclein alpha Homo sapiens 152-167 20596942-4 2010 The results indicate that alpha-synuclein has a dual effect on toxicity of rotenone according to exposure time in human SH-SY5Y cells. Rotenone 75-83 synuclein alpha Homo sapiens 26-41 18639366-3 2010 Pretreatment with B vitamins (also 4 weeks) prevented rotenone-induced: (1) mitochondrial dysfunction, including reduced mitochondrial membrane potential and activities of complex I; (2) oxidative stress, including increase in reactive oxygen species, oxidative DNA damage and protein oxidation, and (3) Parkinsonism parameters, including accumulation of alpha-synuclein and poly-ubiquitin. Rotenone 54-62 synuclein alpha Homo sapiens 355-370 20117172-0 2010 Autophagy protects the rotenone-induced cell death in alpha-synuclein overexpressing SH-SY5Y cells. Rotenone 23-31 synuclein alpha Homo sapiens 54-69 20117172-7 2010 Autophagy activation also restored the mitochondrial membrane potential (MMP) impaired by rotenone in mutant alpha-synuclein expressing cells. Rotenone 90-98 synuclein alpha Homo sapiens 109-124 20117172-8 2010 Additionally, autophagy activation significantly relieved rotenone-induced ROS accumulation and HIF-1alpha expression in neuronal cells expressing mutant alpha-synuclein proteins. Rotenone 58-66 synuclein alpha Homo sapiens 154-169 19681169-3 2010 The rat rotenone model reproduces many of the pathological features of the human disease, including apomorphine-responsive behavioral deficits, DA depletion, loss of striatal DA terminals and nigral dopaminergic neurons, and alpha-synuclein/polyubiquitin-positive cytoplasmic inclusions reminiscent of Lewy bodies. Rotenone 8-16 synuclein alpha Homo sapiens 225-240 19850127-7 2010 We also observed that hVPS41 reduced the accumulation of insoluble high-molecular weight forms of alpha-syn in SH-SY5Y cells after treatment with rotenone. Rotenone 146-154 synuclein alpha Homo sapiens 98-107 19385059-8 2009 Additionally, in rotenone-treated animals, alpha-synuclein and poly-ubiquitin positive aggregates were observed in dopamine neurons of the substantia nigra. Rotenone 17-25 synuclein alpha Homo sapiens 43-58 18562315-3 2008 Following the treatment with rotenone, a mitochondrial complex I inhibitor, wild type alpha-synuclein-overexpressing cells demonstrated intracellular aggregations, which shared a number of features with Lewy bodies, although cells overexpressing the S129A mutant, in which phosphorylation at Ser(129) was blocked, showed few aggregations. Rotenone 29-37 synuclein alpha Homo sapiens 86-101 19198139-4 2008 (1) By the treatment with rotenone, wild type alpha-synuclein overexpressing cells demonstrated intracellular aggregations, which shared a number of features with Lewy bodies. Rotenone 26-34 synuclein alpha Homo sapiens 46-61 19198139-9 2008 (5) In wild-type alpha-synuclein cells treated with rotenone, unfolded protein response (UPR) markers were induced prior to the induction of mitochondrial disruption and caspase-3 activation. Rotenone 52-60 synuclein alpha Homo sapiens 17-32 18562315-4 2008 In wild typealpha-synuclein cells treated with rotenone, the proportion of phosphorylated alpha-synuclein was about 1.6 times higher than that of untreated cells. Rotenone 47-55 synuclein alpha Homo sapiens 12-27 16854843-7 2007 Of those, 114 proteins displayed significant changes in the relative abundance in the complexes associated with alpha-synuclein, DJ-1, or both after rotenone treatment. Rotenone 149-157 synuclein alpha Homo sapiens 112-127 16854843-3 2007 In the current study, we analyzed proteins associated with alpha-synuclein and/or DJ-1 with a robust proteomics technique called stable isotope labeling by amino acids in cell culture (SILAC) in dopaminergic MES cells exposed to rotenone versus controls. Rotenone 229-237 synuclein alpha Homo sapiens 59-74 17041725-0 2006 [Overexpression of alpha-synuclein in SH-SY5Y cells partially protected against oxidative stress induced by rotenone]. Rotenone 108-116 synuclein alpha Homo sapiens 19-34 17041725-5 2006 Both SH-SY5Y and alpha-synuclein overexpressed SH-SY5Y (SH-SY5Y/Syn) cells were treated with various concentrations of rotenone for different time. Rotenone 119-127 synuclein alpha Homo sapiens 17-32 17041725-12 2006 Compared with SH-SY5Y cells, SOD activity in SH-SY5Y/Syn cells was increased distinctly (P<0.05) and alpha-synuclein significantly attenuated rotenone-induced cell apoptosis. Rotenone 145-153 synuclein alpha Homo sapiens 104-119 17041725-13 2006 These results suggest that the alpha-synuclein overexpression in SH-SY5Y cells has a tendency to partially resist oxidative stress induced by rotenone and this response may assist cell survival. Rotenone 142-150 synuclein alpha Homo sapiens 31-46 16439141-0 2006 Intersecting pathways to neurodegeneration in Parkinson"s disease: effects of the pesticide rotenone on DJ-1, alpha-synuclein, and the ubiquitin-proteasome system. Rotenone 92-100 synuclein alpha Homo sapiens 110-125 16439141-4 2006 Chronic rotenone exposure in vivo caused oxidative modification of DJ-1, accumulation of alpha-synuclein, and proteasomal impairment. Rotenone 8-16 synuclein alpha Homo sapiens 89-104 16579629-0 2006 Identification of rotenone-induced modifications in alpha-synuclein using affinity pull-down and tandem mass spectrometry. Rotenone 18-26 synuclein alpha Homo sapiens 52-67 16579629-5 2006 Using this method, we mapped posttranslational modifications of alpha-synuclein from untreated neurons and neurons exposed to rotenone, an inhibitor of mitochondrial complex I. Rotenone 126-134 synuclein alpha Homo sapiens 64-79