PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11168650-1 2001 The effect of rat prolactin (rPRL) on basal and LH-, GH- and T3-mediated testosterone and oestradiol secretion was studied in pubertal rat Leydig cells. Luteinizing Hormone 48-50 prolactin Rattus norvegicus 29-33 11168650-10 2001 While a maximum effective dose of rPRL (200 ng) did not alter LH (25 ng)-induced testosterone and oestradiol secretion, it inhibited testosterone secretion induced by 100 ng LH and increased oestradiol secretion. Luteinizing Hormone 174-176 prolactin Rattus norvegicus 34-38 11168650-12 2001 The present in vitro study indicates that rPRL stimulates both testosterone and oestradiol secretion by Leydig cells and that this effect can be modulated by LH, GH and T3. Luteinizing Hormone 158-160 prolactin Rattus norvegicus 42-46 9687315-12 1998 3) PRL in the culture also dramatically decreased LH-induced tPA mRNA and activity in a dose- and time-dependent fashion. Luteinizing Hormone 50-52 prolactin Rattus norvegicus 3-6 8699143-2 1996 Both testosterone and elevated circulating prolactin (PRL) levels inhibit postcastration LH release. Luteinizing Hormone 89-91 prolactin Rattus norvegicus 43-52 8699143-2 1996 Both testosterone and elevated circulating prolactin (PRL) levels inhibit postcastration LH release. Luteinizing Hormone 89-91 prolactin Rattus norvegicus 54-57 8699143-19 1996 In summary, our results support the hypothesis that the inhibitory effect of PRL on postcastration LH release is mediated by suppression of the activity of NE neurones innervating the ME and E neurones terminating in the MPN which, with time, become refractory to continued PRL exposure. Luteinizing Hormone 99-101 prolactin Rattus norvegicus 77-80 8283440-5 1993 Ovine prolactin significantly reduced LH secretion in all groups from approximately 15 to 48 h after castration. Luteinizing Hormone 38-40 prolactin Rattus norvegicus 6-15 7731494-9 1995 After 7 days of PRL infusion, LH levels had decreased by 45%, whereas plasma corticosterone was 150% higher. Luteinizing Hormone 30-32 prolactin Rattus norvegicus 16-19 7731494-10 1995 This action of PRL on LH and corticosterone was prevented when besides PRL also CRF antiserum was infused. Luteinizing Hormone 22-24 prolactin Rattus norvegicus 15-18 8258648-7 1993 There was a loss of pulsatility of PRL secretion with an increase in the pulse frequency and amplitude of the D-Trp6-LHRH, which was produced parallelly to the desensitization of the gonadotroph to release LH. Luteinizing Hormone 117-119 prolactin Rattus norvegicus 35-38 1472694-1 1992 Posterior hypothalamic lesions restricted to the mammillary body in newborn rats evoked significantly elevated serum prolactin concentrations (P < 0.05) in adult females in the afternoon of proestrous (16.00 h), while at the same time serum LH values appeared significantly depressed (P < 0.05) as compared to controls. Luteinizing Hormone 244-246 prolactin Rattus norvegicus 117-126 2510992-5 1989 The intracellular LH concentration was also increased by PRL. Luteinizing Hormone 18-20 prolactin Rattus norvegicus 57-60 2257614-3 1990 Conversely, prolonged PRL treatment notably increased the gonadotrophic effects of chronic LH administration; this mainly consisted of a rise in the blood concentration of testosterone and a conspicuous hypertrophy of Leydig cells. Luteinizing Hormone 91-93 prolactin Rattus norvegicus 22-25 2257614-6 1990 In the light of these findings, the hypothesis is advanced that the mechanism underlying the gonadotrophic action of PRL involves an enhancement of the endogenous cholesterol synthesis, which could provide an abundance of precursors for testosterone synthesis, the post-cholesterol steps of which, in turn, would be exclusively controlled by LH. Luteinizing Hormone 342-344 prolactin Rattus norvegicus 117-120 2253591-6 1990 The replacement with ovine prolactin by means of a mini-osmotic pump (0.3 mg/day, s.c.) in CB-154-treated lactating rats restored the duration of LH suppression. Luteinizing Hormone 146-148 prolactin Rattus norvegicus 27-36 2559342-1 1989 Hyperprolactinemia suppresses endogenous prolactin (PRL) secretion and inhibits LH release in ovariectomized rats. Luteinizing Hormone 80-82 prolactin Rattus norvegicus 5-14 3006548-2 1986 The addition of prolactin (1 microgram/ml) or dexamethasone (10(-7) M) to the Leydig cell incubation medium induces a 20% increase of the basal estradiol production, whereas, under LH or dbcAMP stimulations, 46 and 41% decreases are noted; moreover, a synergistic effect between prolactin and dexamethasone was observed in presence of dbcAMP leading to a 53% diminution of the estradiol synthesis. Luteinizing Hormone 181-183 prolactin Rattus norvegicus 16-25 2708123-1 1989 The direct peripheral effect of prolactin on LH-stimulated testosterone secretion was re-evaluated using the intact, isolated, perfused rat testis. Luteinizing Hormone 45-47 prolactin Rattus norvegicus 32-41 2708123-4 1989 A dose of 300 ng of LH, which was on the ascending portion of the dose-response curve, was selected so that both stimulatory and inhibitory effects of prolactin could be observed. Luteinizing Hormone 20-22 prolactin Rattus norvegicus 151-160 3242177-1 1988 The role of endogenous prolactin (PRL) in the control of testosterone (T) secretion and T responses to LH treatment was evaluated in adult male rats. Luteinizing Hormone 103-105 prolactin Rattus norvegicus 34-37 4052527-4 1985 Treatment of hypophysectomized animals with Prl alone had no effect on the enzyme activity but enhanced the effect of LH. Luteinizing Hormone 118-120 prolactin Rattus norvegicus 44-47 3933236-9 1985 The magnitude of Prl mediated progesterone secretion observed through 6 days of culturing was dose dependent on the preincubation concentration of FSH or LH. Luteinizing Hormone 154-156 prolactin Rattus norvegicus 17-20 2980106-2 1986 In APTr rats the CL is under the only control of PRL since an almost complete absence of LH and FSH exist. Luteinizing Hormone 89-91 prolactin Rattus norvegicus 49-52 4052527-6 1985 These results indicate that Prl is involved, along with LH, in maintaining the high 5 alpha-reductase activity of the prepubertal rat testis; the action of Prl, apparently requiring the presence of LH, may be to decrease the rate of degradation of the enzyme. Luteinizing Hormone 198-200 prolactin Rattus norvegicus 156-159 4040304-4 1985 These data suggest that Prl has a direct suppressive effect on LH secretion from the pituitary in the rat, and that decreased translocation of ER into the nucleus might relate to impaired LH release by E2 from the pituitary of hyperprolactinaemic rats. Luteinizing Hormone 63-65 prolactin Rattus norvegicus 24-27 3157667-6 1985 These findings also suggest that prolonged exposure to an LH-RH agonist may first produce stimulation, followed by an inhibition of PRL release from both in situ and ectopic pituitaries. Luteinizing Hormone 58-60 prolactin Rattus norvegicus 132-135 3883215-3 1985 In another group of rats, sheep anti-LH-RH serum induced a significant decrease of serum LH and also lowered serum prolactin levels previously elevated by haloperidol. Luteinizing Hormone 37-39 prolactin Rattus norvegicus 115-124 3969813-0 1985 Suppressive effect of prolactin on oestrogen-induced secretion of LH by sequentially perifused rat hypothalamus-pituitary. Luteinizing Hormone 66-68 prolactin Rattus norvegicus 22-31 3969813-7 1985 These data demonstrate that Prl at this concentration suppressed the rapid LH release induced by E2. Luteinizing Hormone 75-77 prolactin Rattus norvegicus 28-31 3883215-4 1985 In ovariectomized rats, sheep anti-LH-RH serum markedly reduced serum LH levels and also decreased serum prolactin elevated by the pretreatment with haloperidol. Luteinizing Hormone 35-37 prolactin Rattus norvegicus 105-114 6291906-3 1982 The addition of highly purified PRL (100 ng/ml) markedly inhibited (98%) the LH-stimulated androsterone accumulation. Luteinizing Hormone 77-79 prolactin Rattus norvegicus 32-35 6541239-8 1984 In some of the "LH-only" group, a possible relationship was examined between the prolactin level at the time of LH injection and the subsequent ability of the rat to sustain multiple cycles. Luteinizing Hormone 16-18 prolactin Rattus norvegicus 81-90 6299687-6 1983 However, when these granulosa cells were treated in vitro or in vivo with FSH and then exposed to LH or PRL, the cells responded to LH in a dose-dependent manner with increased plasminogen activator production. Luteinizing Hormone 132-134 prolactin Rattus norvegicus 104-107 6296383-6 1982 It is suggested that the contribution of PRL in the luteotropic complex is permissive for the luteotropic action of LH and/or for keeping the corpus luteum in a functional state rather than being luteotropic by itself. Luteinizing Hormone 116-118 prolactin Rattus norvegicus 41-44 6094697-3 1984 The parent oestrogens, oestradiol-17 beta and oestrone, had no effect on plasma LH concentrations, but both increased significantly the plasma concentration of prolactin, and oestrone but not oestradiol-17 beta increased the pituitary concentration of LH. Luteinizing Hormone 252-254 prolactin Rattus norvegicus 160-169 6587713-8 1984 Resembling the LH response it was found that PGE2-induced Prl release was greater at 13.00 h than at 09.00 h. In adult male rats the stimulatory effect of PGE2 on LH and Prl release was independent of the time of administration. Luteinizing Hormone 15-17 prolactin Rattus norvegicus 58-61 6606568-5 1983 The plasma PRL levels showed 2.5-fold decrease by 19.30 h. The HYP PRL concentration exhibited 3.5-fold elevation from 01.30 to 05.30 h. After this peak concentration, the HYP PRL showed a 4-fold decrease at 11.30 h. A statistically non-significant rhythm was found in the plasma LH levels. Luteinizing Hormone 280-282 prolactin Rattus norvegicus 11-14 6606568-5 1983 The plasma PRL levels showed 2.5-fold decrease by 19.30 h. The HYP PRL concentration exhibited 3.5-fold elevation from 01.30 to 05.30 h. After this peak concentration, the HYP PRL showed a 4-fold decrease at 11.30 h. A statistically non-significant rhythm was found in the plasma LH levels. Luteinizing Hormone 280-282 prolactin Rattus norvegicus 67-70 6606568-5 1983 The plasma PRL levels showed 2.5-fold decrease by 19.30 h. The HYP PRL concentration exhibited 3.5-fold elevation from 01.30 to 05.30 h. After this peak concentration, the HYP PRL showed a 4-fold decrease at 11.30 h. A statistically non-significant rhythm was found in the plasma LH levels. Luteinizing Hormone 280-282 prolactin Rattus norvegicus 67-70 6300718-1 1983 The present study was concerned with the effects of a transplantable pituitary tumor secreting prolactin (PRL) and adrenocorticotrophin (ACTH) on the levels of LH and FSH in peripheral plasma and on the hypothalamic release of LH-RH and dopamine in the male rat. Luteinizing Hormone 160-162 prolactin Rattus norvegicus 95-104 6300718-1 1983 The present study was concerned with the effects of a transplantable pituitary tumor secreting prolactin (PRL) and adrenocorticotrophin (ACTH) on the levels of LH and FSH in peripheral plasma and on the hypothalamic release of LH-RH and dopamine in the male rat. Luteinizing Hormone 160-162 prolactin Rattus norvegicus 106-109 6291906-6 1982 The effects of PRL on LH-stimulated androgen production were not due to changes in [125I]iodo-hCG binding. Luteinizing Hormone 22-24 prolactin Rattus norvegicus 15-18 6291906-8 1982 As with LH, prostaglandin E2, cholera toxin, or 8-bromo cAMP also caused marked increases in androsterone synthesis, and these effects were blocked (98-99%) by PRL. Luteinizing Hormone 8-10 prolactin Rattus norvegicus 160-163 6291906-9 1982 Studies on the metabolism of steroid hormones revealed that PRL decreased LH-stimulated androsterone and 5 alpha-androstane-3 alpha, 17 beta-diol accumulation by 99%, androstenedione by 94%, testosterone by 90%, dehydroepiandrosterone by more than 80%, pregnenolone and 17 alpha-hydroxyprogesterone by 80%, 17 alpha-hydroxypregnenolone by 84%, and progesterone by 71%. Luteinizing Hormone 74-76 prolactin Rattus norvegicus 60-63 6291906-11 1982 It is inferred from these results that PRL antagonizes the stimulatory effects of LH on ovarian androgen biosynthesis by inhibiting a step distal to cAMP formation and before or at the cholesterol side-chain cleavage step. Luteinizing Hormone 82-84 prolactin Rattus norvegicus 39-42 6286389-8 1982 Likewise, treatment with LH (100 ng/ml) also stimulated PRL-binding capacity by approximately 2-fold. Luteinizing Hormone 25-27 prolactin Rattus norvegicus 56-59 6296803-4 1982 Treatment with the LHRH agonist decreases plasma PRL levels, whereas the antiandrogen increases the concentration of circulating LH and FSH by 250%. Luteinizing Hormone 19-21 prolactin Rattus norvegicus 49-52 6800769-9 1982 Although LH alone had no effect on steroidogenesis, concomitant treatment with LH and PRL resulted in a stimulation of progesterone production that was additive. Luteinizing Hormone 9-11 prolactin Rattus norvegicus 86-89 7327531-2 1981 Prolonged suppression of prolactin (PRL) in immature male rats inhibits the spermatocyte-spermatid conversion process, alters leydig cell morphology, decreases accessory sex organ weight and increases serum LH levels without a significant alteration in serum FSH concentration. Luteinizing Hormone 207-209 prolactin Rattus norvegicus 25-34 743999-4 1978 The level of LH achieved was inversely correlated with the PRL concentration generated (r = -0.71; P less than 0.01). Luteinizing Hormone 13-15 prolactin Rattus norvegicus 59-62 6777214-7 1980 Concomitant treatment with PRL suppressed the FSH- and LH-induced increases in estrogen production in a dose-dependent manner; 1 micrograms/ml PRL suppressed estrogen production by > 80% during days 2-4 of culture. Luteinizing Hormone 55-57 prolactin Rattus norvegicus 27-30 6777214-7 1980 Concomitant treatment with PRL suppressed the FSH- and LH-induced increases in estrogen production in a dose-dependent manner; 1 micrograms/ml PRL suppressed estrogen production by > 80% during days 2-4 of culture. Luteinizing Hormone 55-57 prolactin Rattus norvegicus 143-146 7190891-6 1980 These results imply that there probably is a quantitative relationship between the duration (as well as time of onset) of the mutually opposing effects of PRL and the uterus on the appearance of LH dependency. Luteinizing Hormone 195-197 prolactin Rattus norvegicus 155-158 574418-14 1979 Hihgh serum prolactin levels may stimulate DA turnover which is inhibitory to pituitary LH release, thus counteracting the stimulatory effect of NE on LH-RH release. Luteinizing Hormone 88-90 prolactin Rattus norvegicus 12-21 743999-7 1978 These experiments support the hypothesis that PRL directly inhibits LH secretion, presumably at the pituitary level. Luteinizing Hormone 68-70 prolactin Rattus norvegicus 46-49 1108288-6 1975 An injection of 2 ng of synthetic LH-RH into the pituitary following 0.1 mug of estradiol benzoate resulted in a fall of serum LH and FSH levels, and a rise of serum prolactin level. Luteinizing Hormone 34-36 prolactin Rattus norvegicus 166-175 874418-1 1977 Increase of plasma prolactin in chronically ovariectomized rats by transplanting pituitary glands from male rats to the kidney capsule reduced pituitary LH output for 1 week. Luteinizing Hormone 153-155 prolactin Rattus norvegicus 19-28 874418-10 1977 The mechanism by which chronically high prolactin levels cause apomorphine to be less effective in reducing pituitary LH release may be a desensitization of some dopamine-receptive mechanism which is inhibitory to LH release. Luteinizing Hormone 118-120 prolactin Rattus norvegicus 40-49 190066-4 1977 The localization of prolactin receptors interstitial tissue suggests that the effect of prolactin on LH/hCG-stimulated testosterone production is due to a direct effect of prolactin of Leydig cells. Luteinizing Hormone 101-103 prolactin Rattus norvegicus 20-29 190066-4 1977 The localization of prolactin receptors interstitial tissue suggests that the effect of prolactin on LH/hCG-stimulated testosterone production is due to a direct effect of prolactin of Leydig cells. Luteinizing Hormone 101-103 prolactin Rattus norvegicus 88-97 798686-1 1976 Synthetic LHRH, at a dose of 5 mug/kg, induced a marked increase in the serum LH level but a marked decrease in the serum prolactin level when injected iv to normal adult female rats on the day of estrus. Luteinizing Hormone 10-12 prolactin Rattus norvegicus 122-131 174897-7 1976 The cells released significant amounts of PRL, TSH, and to a lesser extent, LH, in response to 1-5 X 10-3M N6,O2"-dibutyryl cyclic AMP, accompanied by remarkable elevation in total content (cells + medium) of PRL and TSH but not of LH. Luteinizing Hormone 232-234 prolactin Rattus norvegicus 42-45 1028951-0 1976 Selective actions of prolactin on catecholamine turnover in the hypothalamus and on serum LH and FSH. Luteinizing Hormone 90-92 prolactin Rattus norvegicus 21-30 1028951-3 1976 However, PRL selectively enhanced dopamine turnover in the median eminence and anterior hypothalamus after a latent period of 10-26 h. In addition, PRL administration significantly decreased serum concentrations of LH and FSH. Luteinizing Hormone 215-217 prolactin Rattus norvegicus 9-12 1028951-3 1976 However, PRL selectively enhanced dopamine turnover in the median eminence and anterior hypothalamus after a latent period of 10-26 h. In addition, PRL administration significantly decreased serum concentrations of LH and FSH. Luteinizing Hormone 215-217 prolactin Rattus norvegicus 148-151 1028951-4 1976 These results suggest that the PRL-induced increase in activity of dopaminergic neurons in the median eminence or anterior hypothalamus may be responsible for the reduction of the post-castration rise in serum concentrations of LH and FSH. Luteinizing Hormone 228-230 prolactin Rattus norvegicus 31-34 401033-6 1977 Plasma LH levels rose gradually after prolactin implantation, and in those rats in which ovulation occurred, a preovulatory LH rise was detected at 1800 h on the previous day. Luteinizing Hormone 7-9 prolactin Rattus norvegicus 38-47 190066-4 1977 The localization of prolactin receptors interstitial tissue suggests that the effect of prolactin on LH/hCG-stimulated testosterone production is due to a direct effect of prolactin of Leydig cells. Luteinizing Hormone 101-103 prolactin Rattus norvegicus 88-97 187413-4 1976 Administration of LH caused receptor for LH to decrease markedly within 24 h and to remain low for 96 h. In contrast, granulosa cell content of receptor for PRL increased progressively for 48 h following LH stimulation and remained elevated in fully luteinized cells at 96 h. This increase in PRL receptor appears to be functionally related to the ability of luteal cells to respond to PRL. Luteinizing Hormone 18-20 prolactin Rattus norvegicus 293-296 187413-4 1976 Administration of LH caused receptor for LH to decrease markedly within 24 h and to remain low for 96 h. In contrast, granulosa cell content of receptor for PRL increased progressively for 48 h following LH stimulation and remained elevated in fully luteinized cells at 96 h. This increase in PRL receptor appears to be functionally related to the ability of luteal cells to respond to PRL. Luteinizing Hormone 18-20 prolactin Rattus norvegicus 293-296 187413-5 1976 When PRL was given for 4 days after LH, both luteal cell progesterone production and LH receptor content increased progressively after, but not before, 48 h. Since these changes occurred in the absence of LH, the increase in LH receptor appears to be a consequence of, but not a requirement for, the PRL-induced increase in progesterone production. Luteinizing Hormone 36-38 prolactin Rattus norvegicus 5-8 187413-5 1976 When PRL was given for 4 days after LH, both luteal cell progesterone production and LH receptor content increased progressively after, but not before, 48 h. Since these changes occurred in the absence of LH, the increase in LH receptor appears to be a consequence of, but not a requirement for, the PRL-induced increase in progesterone production. Luteinizing Hormone 85-87 prolactin Rattus norvegicus 5-8 187413-6 1976 If daily injections of PRL were delayed for 72 or 96 h following LH induction of lutenization, luteolytic rather than luteotropic effects of PRL were observed. Luteinizing Hormone 65-67 prolactin Rattus norvegicus 23-26 179793-3 1976 Dispersed luteal cells previously exposed in vivo to biphasic prolactin (PRL) surges were found to respond during perifusion to as little as 0.5 ng/ml LH by increased steroid secretion. Luteinizing Hormone 151-153 prolactin Rattus norvegicus 62-71 24456164-0 2014 Prolactin regulates kisspeptin neurons in the arcuate nucleus to suppress LH secretion in female rats. Luteinizing Hormone 74-76 prolactin Rattus norvegicus 0-9 167373-5 1975 Inhibition curves and tests of cross reactivity with LH, FSH, TSH, and GH showed that binding of PRL to its receptors in the kidneys and adrenals was specific. Luteinizing Hormone 53-55 prolactin Rattus norvegicus 97-100 1129260-2 1975 Plasma levels of PRL and TSH were elevated (three- to fivefold) in cesarean sectioned mothers given 4-5 sc injections of PGE-1; higher doses also stimulated LH release. Luteinizing Hormone 157-159 prolactin Rattus norvegicus 17-20 25993525-9 2015 These data serve as the first evidence to suggest that PRL signaling through hypothalamic PRLR inhibits pulsatile secretion of LH in a gamma-aminobutyric acid receptor type A-dependent fashion and tonically restrains the magnitude of the LH surge. Luteinizing Hormone 127-129 prolactin Rattus norvegicus 55-58 25993525-9 2015 These data serve as the first evidence to suggest that PRL signaling through hypothalamic PRLR inhibits pulsatile secretion of LH in a gamma-aminobutyric acid receptor type A-dependent fashion and tonically restrains the magnitude of the LH surge. Luteinizing Hormone 238-240 prolactin Rattus norvegicus 55-58 24456164-3 2014 We investigated whether PRL acts on kisspeptin neurons to suppress LH secretion in lactating (Lac) and virgin rats. Luteinizing Hormone 67-69 prolactin Rattus norvegicus 24-27 22674474-12 2012 These results show that the effect of centrally injected allopreganolone over reproductive function could be due to a centrally originated LH mediated effect over ovarian function that affects luteal regression, through the inhibition of apoptosis and stimulation of progesterone and prolactin release. Luteinizing Hormone 139-141 prolactin Rattus norvegicus 284-293 20236228-8 2010 However, rPRL prevented the Br-induced increase in basal and GnRH-stimulated LH output, and suppressed LH below basal values (P < 0.05). Luteinizing Hormone 77-79 prolactin Rattus norvegicus 9-13