PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10747047-6 2000 However, when grown in liquid culture in medium containing limiting amounts of inositol, the opi1Delta ino4Delta strain exhibited a level of INO1 expression comparable to, or higher than, the wild-type strain growing under the same conditions. Inositol 79-87 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 93-97 10747047-7 2000 Furthermore, INO1 expression in the opi1Delta ino4Delta strain was repressed in cells grown in medium fully supplemented with both inositol and choline. Inositol 131-139 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 36-40 10407264-10 1999 Overexpression of OPI1 under control of the GAL1 promoter severely inhibited activation of ICRE-dependent genes, leading to inositol-requiring cells. Inositol 124-132 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 18-22 10224244-3 1999 Dominant mutations in SNF4 and recessive mutations in REG1, OPI1, and RTF2 were isolated that specifically suppress the inositol auxotrophy of the TBP mutant strains. Inositol 120-128 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 60-64 9802015-0 1998 Pleiotropic effects of the opi1 regulatory mutation of yeast: its effects on growth and on phospholipid and inositol metabolism. Inositol 108-116 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 27-31 9802015-7 1998 Intracellular inositol contents of the opi1 strain were higher than those of the wild-type strain, with peak levels occurring in the stationary phase. Inositol 14-22 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 39-43 9802015-8 1998 Membrane phosphatidylinositol content paralleled intracellular inositol content, with opi1 strains having a higher phosphatidylinositol content in stationary phase. Inositol 21-29 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 86-90 9611229-3 1998 In the presence of inositol, transcription is repressed by Opi1p. Inositol 19-27 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 59-64 8614637-3 1996 Repression in response to inositol supplementation requires the OPI1 regulatory gene. Inositol 26-34 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 64-68 7568205-3 1995 However, it was also known that cells which harbor a mutant opi1 allele express constitutively derepressed levels of target genes (INO1 and CHO1), implicating the OPI1 negative regulatory gene in the response to inositol. Inositol 212-220 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 60-64 7568205-3 1995 However, it was also known that cells which harbor a mutant opi1 allele express constitutively derepressed levels of target genes (INO1 and CHO1), implicating the OPI1 negative regulatory gene in the response to inositol. Inositol 212-220 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 163-167 7568205-4 1995 These observations suggested that the response to inositol may involve both regulation of INO2 transcription as well as OPI1-mediated repression. Inositol 50-58 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 120-124 7568205-9 1995 Furthermore, the effect of inositol on target gene expression was eliminated by deleting the OPI1 gene in the GAL1-INO2-containing strain. Inositol 27-35 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 93-97 7568205-10 1995 These data suggest that the OPI1 gene product is the primary target (sensor) of the inositol response and that derepression of INO2 transcription determines the degree of expression of the target genes. Inositol 84-92 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 28-32 7768846-5 1995 In a strain in which a negative regulator of phospholipid and inositol biosynthesis had been deleted (an opi1 mutant), this pattern of extracellular GroPIns accumulation in response to inositol availability was altered. Inositol 62-70 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 105-109 7768846-5 1995 In a strain in which a negative regulator of phospholipid and inositol biosynthesis had been deleted (an opi1 mutant), this pattern of extracellular GroPIns accumulation in response to inositol availability was altered. Inositol 185-193 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 105-109 7753636-9 1995 Mutation of the specific negative regulator of phospholipid synthesis encoded by OPI1 suppressed the inositol auxotrophy of swi2 mutants. Inositol 101-109 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 81-85 7862162-3 1995 In the presence of inositol and choline (repressing), the product of the OPI1 gene represses transcription dictated by the UASINO element. Inositol 19-27 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 73-77 8294482-6 1994 INO2 and INO4 are required for derepressed levels of ITR1 mRNA, and OPI1 is necessary for repression of transcript levels in response to inositol. Inositol 137-145 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 68-72 8294482-7 1994 The INO2, INO4, and OPI1 genes thus coordinate uptake of inositol to endogenous inositol biosynthesis and to phospholipid biosynthesis. Inositol 57-65 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 20-24 8294482-7 1994 The INO2, INO4, and OPI1 genes thus coordinate uptake of inositol to endogenous inositol biosynthesis and to phospholipid biosynthesis. Inositol 80-88 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 20-24 2850468-8 1988 PGPS expression in the opi1 mutant, which exhibits constitutive synthesis of general phospholipid biosynthetic enzymes, was fully repressed in the presence of inositol and partially repressed even in the absence of inositol. Inositol 159-167 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 23-27 2850468-8 1988 PGPS expression in the opi1 mutant, which exhibits constitutive synthesis of general phospholipid biosynthetic enzymes, was fully repressed in the presence of inositol and partially repressed even in the absence of inositol. Inositol 215-223 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 23-27 3023284-6 1986 VAL2C(YEp CHO1) is a plasmid-bearing strain that over produces phosphatidylserine synthase activity, and the opi1 mutant is an inositol biosynthesis regulatory mutant. Inositol 127-135 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 109-113 3025587-8 1986 The transcript was constitutively overproduced in cells containing a mutation (opi1) that causes constitutive expression of inositol-1-phosphate synthase and results in excretion of inositol. Inositol 124-132 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 79-83 3888957-1 1985 Phospholipid metabolism in the Saccharomyces cerevisiae opi1 mutant, which excretes inositol and is constitutive for the biosynthetic enzyme inositol-1-phosphate synthase (M. Greenberg, P. Goldwasser, and S. Henry, Mol. Inositol 84-92 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 56-60 7047296-4 1982 Genetic analysis of the mutants indicates that at least three loci (designated OPI1, OPI2 and OPI4) direct inositol-mediated repression of I-1-P synthase. Inositol 107-115 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 79-83 33931115-3 2021 RESULTS: The opi1 gene was knocked out because of its negative regulation on myo-inositol synthesis, which is the limiting step of D-glucaric acid production by S. cerevisiae. Inositol 77-89 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 13-17 28827025-6 2018 The regulation of the CHO1 expression is mediated through the inositol-sensitive upstream activation sequence (UASINO), a cis-acting element for the phosphatidate-controlled Henry (Ino2-Ino4/Opi1) regulatory circuit. Inositol 62-70 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 191-195 28924045-2 2017 Inositol depletion favors Opi1p interaction with both Scs2p and phosphatidic acid at the endoplasmic reticulum (ER) membrane. Inositol 0-8 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 26-31 28924045-3 2017 Inositol supplementation, however, favors the translocation of Opi1p from the ER into the nucleus, where it interacts with the Ino2p-Ino4p complex, attenuating transcription of INO1 A strain devoid of Scs2p (scs2Delta) and a mutant, OPI1FFAT, lacking the ability to interact with Scs2p were utilized to examine the specific role(s) of the Opi1p-Scs2p interaction in the regulation of INO1 expression and overall lipid metabolism. Inositol 0-8 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 63-68 28924045-3 2017 Inositol supplementation, however, favors the translocation of Opi1p from the ER into the nucleus, where it interacts with the Ino2p-Ino4p complex, attenuating transcription of INO1 A strain devoid of Scs2p (scs2Delta) and a mutant, OPI1FFAT, lacking the ability to interact with Scs2p were utilized to examine the specific role(s) of the Opi1p-Scs2p interaction in the regulation of INO1 expression and overall lipid metabolism. Inositol 0-8 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 339-344 28924045-4 2017 Loss of the Opi1p-Scs2p interaction reduced INO1 expression and conferred inositol auxotrophy. Inositol 74-82 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 12-17 28924045-5 2017 Moreover, inositol depletion in strains lacking this interaction resulted in Opi1p being localized to sites of lipid droplet formation, coincident with increased synthesis of triacylglycerol. Inositol 10-18 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 77-82 28581036-3 2017 Opi1p is a transcriptional repressor that is inactive when present at the nuclear membrane/endoplasmic reticulum, but represseses transcription of inositol upstream activating sequence (UASINO )-containing genes, many of which are involved in the synthesis of phospholipids and fatty acids, when it is translocated to the nucleus. Inositol 147-155 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 0-5 28175933-2 2017 We have previously shown that the negative regulator Opi1 of yeast phospholipid biosynthesis inhibits transcription by recruiting corepressors Sin3 and Cyc8 in the presence of precursor molecules inositol and choline. Inositol 196-204 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 53-57 25602740-3 2015 However, in the more distantly related yeast Yarrowia lipolytica, the OPI1 homolog was not found to regulate inositol biosynthesis, but alkane oxidation. Inositol 109-117 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 70-74 25602740-4 2015 In Candida albicans, the most common cause of human candidiasis, its Opi1p homolog, CaOpi1p, has been shown to complement a S. cerevisiae opi1 mutant for inositol biosynthesis regulation when heterologously expressed, suggesting it might serve a similar role in this pathogen. Inositol 155-163 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 138-142 22543816-1 2012 Yeast genes of phospholipid biosynthesis are negatively regulated by repressor protein Opi1 when precursor molecules inositol and choline (IC) are available. Inositol 117-125 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 87-91 22345606-6 2012 Cellular levels of phosphatidic acid, precursor to all membrane phospholipids and the storage lipid triacylglycerol, regulates transcription of UAS(INO)-containing genes by tethering Opi1 to the nuclear/endoplasmic reticulum membrane and controlling its translocation into the nucleus, a mechanism largely controlled by inositol availability. Inositol 320-328 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 183-187 21372176-3 2011 Cells grown in the absence of inositol sequester Scs2p-Opi1p at the ER and derepress target genes including INO1. Inositol 30-38 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 55-60 21372176-6 2011 Yet complex binding to Scs2p-Opi1p was enhanced by inositol starvation, although the interaction between Scs2p and Opi1p was not influenced by YET1 or YET3 deletion. Inositol 51-59 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 29-34 21104417-1 2011 Repressor protein Opi1 is required to negatively regulate yeast structural genes of phospholipid biosynthesis in the presence of precursor molecules inositol and choline (IC). Inositol 149-157 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 18-22 18842580-3 2008 Mutants defective in this pathway exhibit a choline-sensitive inositol auxotrophy, yet fully derepress INO1 and other Opi1p-regulated genes when grown in the absence of inositol. Inositol 169-177 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 118-123 19812714-5 2007 The OPI1 gene product is a negative regulatory factor that controls the transcription of the INO1 structural gene, which encodes the enzyme catalyzing the limiting step in the biosynthesis of inositol, that is, the conversion of glucose-6-phosphate to inositol-3-phosphate. Inositol 192-200 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 4-8 19812714-6 2007 Upon the deletion of the OPI1 gene, the cell will constitutively produce inositol, regardless of the extracellular inositol concentration. Inositol 73-81 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 25-29 19812714-6 2007 Upon the deletion of the OPI1 gene, the cell will constitutively produce inositol, regardless of the extracellular inositol concentration. Inositol 115-123 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 25-29 19812714-9 2007 The opi1 strain, with the ability to constitutively produce inositol regardless of media composition, showed less inhibition of cell growth in the presence of ethanol than did the wild-type strain, particularly in inositol-free media. Inositol 60-68 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 4-8 19812714-9 2007 The opi1 strain, with the ability to constitutively produce inositol regardless of media composition, showed less inhibition of cell growth in the presence of ethanol than did the wild-type strain, particularly in inositol-free media. Inositol 214-222 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 4-8 19812714-10 2007 We conclude that the introduction of an opi1 mutation in yeast results in an inherent increase in PI levels and constitutive biosynthesis of inositol that, in turn, will reduce the cost of supplementing inositol into the media to achieve a higher ethanol tolerance. Inositol 141-149 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 40-44 19812714-10 2007 We conclude that the introduction of an opi1 mutation in yeast results in an inherent increase in PI levels and constitutive biosynthesis of inositol that, in turn, will reduce the cost of supplementing inositol into the media to achieve a higher ethanol tolerance. Inositol 203-211 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 40-44 16807089-8 2007 In contrast, the negative regulatory protein Opi1p, which is involved in inositol-mediated regulation of phospholipid synthesis, represses the expression of the CHO1 gene through the cis-acting element UAS(INO). Inositol 73-81 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 45-50 16777852-5 2006 We also report that the unfolded protein response pathway is rapidly inactivated by inositol supplementation and demonstrate that the response of the unfolded protein response pathway to inositol is separable from the response mediated by Opi1p. Inositol 187-195 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 239-244 16582425-4 2006 In the presence of inositol, PA levels decrease, releasing Opi1p into the nucleus where it represses transcription. Inositol 19-27 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 59-64 16582425-5 2006 The opi1 mutant overproduces and excretes inositol into the growth medium in the absence of inositol and choline (Opi(-) phenotype). Inositol 42-50 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 4-8 16582425-5 2006 The opi1 mutant overproduces and excretes inositol into the growth medium in the absence of inositol and choline (Opi(-) phenotype). Inositol 92-100 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 4-8 16407309-2 2006 Opi1p repressor activity is most active in inositol-supplemented cells. Inositol 43-51 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 0-5 16407309-8 2006 Expression of the OPI1 allele in an opi1Delta mutant attenuated (2-fold) the repressive effect of Opi1p on INO1 expression, and this effect was only observed when cells were grown in the absence of inositol. Inositol 198-206 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 18-22 15755922-3 2005 Most of the phospholipid biosynthetic genes are regulated in response to inositol and choline via a regulatory circuit that includes the Ino2p:Ino4p activator complex and the Opi1p repressor. Inositol 73-81 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 175-180 15192221-4 2004 After the addition of the lipid precursor inositol, this phosphatidic acid was rapidly consumed, releasing Opi1p from the endoplasmic reticulum and allowing its nuclear translocation and repression of target genes. Inositol 42-50 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 107-112 15165231-2 2004 Conversely, the OPI1 gene is required for repression in response to inositol supplementation. Inositol 68-76 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 16-20 15028711-7 2004 This regulation was mediated through the UAS(INO) element and by the transcription factors Ino2p, Ino4p, and Opi1p that are responsible for the inositol-mediated regulation of UAS(INO)-containing genes involved in phospholipid synthesis. Inositol 144-152 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 109-114 16233644-3 2004 In the presence of inositol and choline, the expression of these genes is downregulated and a functional OPI1 gene product is necessary for this repression. Inositol 19-27 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 105-109 16233644-4 2004 The promoter region of OPI1 contains one copy of ICRE, and here we analyzed the involvement of ICRE in the inositol-choline-mediated gene regulation of OPI1. Inositol 107-115 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 23-27 16233644-4 2004 The promoter region of OPI1 contains one copy of ICRE, and here we analyzed the involvement of ICRE in the inositol-choline-mediated gene regulation of OPI1. Inositol 107-115 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 152-156 14587102-2 2003 Opi1 is a negative regulator responsible for repression of ICRE-dependent genes in the presence of an excess of inositol and choline. Inositol 112-120 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 0-4 12668681-8 2003 The beta-galactosidase activity driven by an INO1-CYC-lacZ reporter gene in opi1Delta mutant cells expressing the S31A and S251A mutant Opi1p proteins was elevated 42 and 35%, respectively, in the absence of inositol and 55 and 52%, respectively, in the presence of inositol when compared with cells expressing wild type Opi1p. Inositol 208-216 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 136-141 12668681-8 2003 The beta-galactosidase activity driven by an INO1-CYC-lacZ reporter gene in opi1Delta mutant cells expressing the S31A and S251A mutant Opi1p proteins was elevated 42 and 35%, respectively, in the absence of inositol and 55 and 52%, respectively, in the presence of inositol when compared with cells expressing wild type Opi1p. Inositol 266-274 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 136-141 16233438-6 2003 A disruptant of the OPI1 gene, an inositol/choline-mediated negative regulatory gene, produced higher amounts of MCFA than the control strain both in the static culture and in sake mash when a sufficient amount of inositol was supplemented. Inositol 34-42 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 20-24 11454208-2 2001 Gene activation by an ICRE is mediated by binding of the Ino2/Ino4 transcription factor, whereas repression in the presence of high concentrations of inositol and choline (IC) requires an intact Opi1 repressor. Inositol 150-158 transcriptional regulator OPI1 Saccharomyces cerevisiae S288C 195-199