PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10644509-5 2000 PKD activation induced by LPA was attenuated markedly and selectively by prior exposure of either Swiss 3T3 or Rat-1 cells to pertussis toxin (PTx) in a concentration-dependent manner. lysophosphatidic acid 26-29 protein kinase D1 Mus musculus 0-3 10644509-0 2000 Lysophosphatidic acid rapidly induces protein kinase D activation through a pertussis toxin-sensitive pathway. lysophosphatidic acid 0-21 protein kinase D1 Mus musculus 38-54 10644509-2 2000 Herein we demonstrate that lysophosphatidic acid (LPA) induces rapid PKD activation in mouse Swiss 3T3 and Rat-1 cells. lysophosphatidic acid 27-48 protein kinase D1 Mus musculus 69-72 10644509-2 2000 Herein we demonstrate that lysophosphatidic acid (LPA) induces rapid PKD activation in mouse Swiss 3T3 and Rat-1 cells. lysophosphatidic acid 50-53 protein kinase D1 Mus musculus 69-72 10644509-3 2000 LPA induced PKD activation in a concentration-dependent fashion with maximal stimulation (7.6-fold) achieved at 5 microM. lysophosphatidic acid 0-3 protein kinase D1 Mus musculus 12-15 10644509-4 2000 Treatment of Swiss 3T3 cells with the protein kinase C (PKC) inhibitors GF-I, Ro-31-8220, and Go-7874 completely abrogated PKD activation induced by LPA at concentrations that did not inhibit PKD activity when added directly to the in vitro kinase assays. lysophosphatidic acid 149-152 protein kinase D1 Mus musculus 123-126 10644509-4 2000 Treatment of Swiss 3T3 cells with the protein kinase C (PKC) inhibitors GF-I, Ro-31-8220, and Go-7874 completely abrogated PKD activation induced by LPA at concentrations that did not inhibit PKD activity when added directly to the in vitro kinase assays. lysophosphatidic acid 149-152 protein kinase D1 Mus musculus 192-195 12477719-0 2003 Cooperation of Gq, Gi, and G12/13 in protein kinase D activation and phosphorylation induced by lysophosphatidic acid. lysophosphatidic acid 96-117 protein kinase D1 Mus musculus 37-53 12477719-1 2003 To examine the contribution of different G-protein pathways to lysophosphatidic acid (LPA)-induced protein kinase D (PKD) activation, we tested the effect of LPA on PKD activity in murine embryonic cell lines deficient in Galpha(q/11) (Galpha(q/11) KO cells) or Galpha(12/13) (Galpha(12/13) KO cells) and used cells lacking rhodopsin kinase (RK cells) as a control. lysophosphatidic acid 63-84 protein kinase D1 Mus musculus 99-115 12477719-1 2003 To examine the contribution of different G-protein pathways to lysophosphatidic acid (LPA)-induced protein kinase D (PKD) activation, we tested the effect of LPA on PKD activity in murine embryonic cell lines deficient in Galpha(q/11) (Galpha(q/11) KO cells) or Galpha(12/13) (Galpha(12/13) KO cells) and used cells lacking rhodopsin kinase (RK cells) as a control. lysophosphatidic acid 63-84 protein kinase D1 Mus musculus 117-120 12477719-1 2003 To examine the contribution of different G-protein pathways to lysophosphatidic acid (LPA)-induced protein kinase D (PKD) activation, we tested the effect of LPA on PKD activity in murine embryonic cell lines deficient in Galpha(q/11) (Galpha(q/11) KO cells) or Galpha(12/13) (Galpha(12/13) KO cells) and used cells lacking rhodopsin kinase (RK cells) as a control. lysophosphatidic acid 86-89 protein kinase D1 Mus musculus 99-115 12477719-1 2003 To examine the contribution of different G-protein pathways to lysophosphatidic acid (LPA)-induced protein kinase D (PKD) activation, we tested the effect of LPA on PKD activity in murine embryonic cell lines deficient in Galpha(q/11) (Galpha(q/11) KO cells) or Galpha(12/13) (Galpha(12/13) KO cells) and used cells lacking rhodopsin kinase (RK cells) as a control. lysophosphatidic acid 86-89 protein kinase D1 Mus musculus 117-120 12477719-2 2003 In RK and Galpha(12/13) KO cells, LPA induced PKD activation through a phospholipase C/protein kinase C pathway in a concentration-dependent fashion with maximal stimulation (6-fold for RK cells and 4-fold for Galpha(12/13) KO cells in autophosphorylation activity) achieved at 3 microm. lysophosphatidic acid 34-37 protein kinase D1 Mus musculus 46-49 12477719-4 2003 However, LPA induced a significantly increased PKD activity when Galpha(q/11) KO cells were transfected with Galpha(q). lysophosphatidic acid 9-12 protein kinase D1 Mus musculus 47-50 12477719-5 2003 LPA-induced PKD activation was modestly attenuated by prior exposure of RK cells to pertussis toxin (PTx) but abolished by the combination treatments of PTx and Clostridium difficile toxin B. lysophosphatidic acid 0-3 protein kinase D1 Mus musculus 12-15 12477719-6 2003 Surprisingly, PTx alone strikingly inhibited LPA-induced PKD activation in a concentration-dependent fashion in Galpha(12/13) KO cells. lysophosphatidic acid 45-48 protein kinase D1 Mus musculus 57-60 12477719-8 2003 Our results indicate that G(q) is necessary but not sufficient to mediate LPA-induced PKD activation. lysophosphatidic acid 74-77 protein kinase D1 Mus musculus 86-89 12477719-10 2003 We conclude that LPA induces PKD activation through G(q), G(i), and G(12) and propose that PKD activation is a point of convergence in the action of multiple G-protein pathways. lysophosphatidic acid 17-20 protein kinase D1 Mus musculus 29-32 12477719-10 2003 We conclude that LPA induces PKD activation through G(q), G(i), and G(12) and propose that PKD activation is a point of convergence in the action of multiple G-protein pathways. lysophosphatidic acid 17-20 protein kinase D1 Mus musculus 91-94 34478715-8 2021 Our results demonstrated that SMCs isolated from LPA receptor 1 (LPA1)-deficient mice completely lost responsiveness to LPA stimulation, which mediates induction of TF expression and activation of PKD and p38/JNK MAPK, indicating that LPA1 is responsible for PKD2-mediated activation of JNK2 and p38alpha. lysophosphatidic acid 120-123 protein kinase D1 Mus musculus 197-200 34478715-4 2021 In the current study, we found that LPA markedly induced protein kinase D (PKD) activation in mouse aortic smooth muscle cells (MASMCs). lysophosphatidic acid 36-39 protein kinase D1 Mus musculus 57-73 34478715-4 2021 In the current study, we found that LPA markedly induced protein kinase D (PKD) activation in mouse aortic smooth muscle cells (MASMCs). lysophosphatidic acid 36-39 protein kinase D1 Mus musculus 75-78 34478715-6 2021 Furthermore, our data reveal a novel finding that PKD2 mediates LPA-induced TF expression via the p38alpha and JNK2 MAPK signaling pathways, which are accompanied by the PKD-independent MEK1/2-ERK-JNK pathway. lysophosphatidic acid 64-67 protein kinase D1 Mus musculus 170-173 23401561-6 2013 Thrombin, lysophosphatidic acid, and S1P increase COX-2 protein expression through a mechanism involving RhoA, catalytically active PLCepsilon, sustained activation of protein kinase D (PKD), and nuclear translocation of NF-kappaB. lysophosphatidic acid 10-31 protein kinase D1 Mus musculus 168-184 23401561-6 2013 Thrombin, lysophosphatidic acid, and S1P increase COX-2 protein expression through a mechanism involving RhoA, catalytically active PLCepsilon, sustained activation of protein kinase D (PKD), and nuclear translocation of NF-kappaB. lysophosphatidic acid 10-31 protein kinase D1 Mus musculus 186-189