PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22537771-6 2012 L-4F inhibited the expression and activity of HIF-1alpha induced by low oxygen concentration, cobalt chloride (CoCl(2), a hypoxia-mimic compound), lysophosphatidic acid, and insulin in two human ovarian cancer cell lines, OV2008 and CAOV-3. lysophosphatidic acid 147-168 hypoxia inducible factor 1 subunit alpha Homo sapiens 46-56 23880760-0 2013 Regulation of hypoxia-inducible factor 1alpha (HIF-1alpha) by lysophosphatidic acid is dependent on interplay between p53 and Kruppel-like factor 5. lysophosphatidic acid 62-83 hypoxia inducible factor 1 subunit alpha Homo sapiens 14-45 23880760-0 2013 Regulation of hypoxia-inducible factor 1alpha (HIF-1alpha) by lysophosphatidic acid is dependent on interplay between p53 and Kruppel-like factor 5. lysophosphatidic acid 62-83 hypoxia inducible factor 1 subunit alpha Homo sapiens 47-57 23880760-3 2013 We have shown previously that LPA stimulates HIF-1alpha expression in colon cancer cells. lysophosphatidic acid 30-33 hypoxia inducible factor 1 subunit alpha Homo sapiens 45-55 23880760-4 2013 To determine the mechanism of HIF-1alpha induction by LPA, we compared the effect of LPA on HIF-1alpha in several colon cancer cell lines. lysophosphatidic acid 54-57 hypoxia inducible factor 1 subunit alpha Homo sapiens 30-40 23880760-6 2013 HIF-1alpha induction was observed in cells expressing WT p53, where LPA decreased p53 expression. lysophosphatidic acid 68-71 hypoxia inducible factor 1 subunit alpha Homo sapiens 0-10 23880760-10 2013 Because HIF-1alpha was necessary for LPA-induced growth of colon cancer cells, we determined the relationship between KLF5 and HIF-1alpha by a loss-of-function approach. lysophosphatidic acid 37-40 hypoxia inducible factor 1 subunit alpha Homo sapiens 8-18 23880760-11 2013 Silencing of KLF5 inhibited LPA-induced HIF-1alpha induction, suggesting that KLF5 is an upstream regulator of HIF-1alpha. lysophosphatidic acid 28-31 hypoxia inducible factor 1 subunit alpha Homo sapiens 40-50 23880760-11 2013 Silencing of KLF5 inhibited LPA-induced HIF-1alpha induction, suggesting that KLF5 is an upstream regulator of HIF-1alpha. lysophosphatidic acid 28-31 hypoxia inducible factor 1 subunit alpha Homo sapiens 111-121 23880760-13 2013 LPA increased the occupancy of the Hif1alpha promoter by KLF5, while decreasing p53 binding. lysophosphatidic acid 0-3 hypoxia inducible factor 1 subunit alpha Homo sapiens 35-44 23880760-15 2013 These results identify KLF5 as a transactivator of HIF-1alpha and show that LPA regulates HIF-1alpha by dynamically modulating its interaction with KLF5 and p53. lysophosphatidic acid 76-79 hypoxia inducible factor 1 subunit alpha Homo sapiens 90-100 23831623-3 2013 In this study, we first confirmed the ability of LPA to induce VEGF-A expression in PC-3 cells and then validated that LPA-induced VEGF-A expression was regulated by HIF-1alpha and ARNT through phosphatidylinositol 3-kinase activation. lysophosphatidic acid 119-122 hypoxia inducible factor 1 subunit alpha Homo sapiens 166-176 23831623-5 2013 Since ARNT is a common dimerization partner of AHR and HIF-1alpha, we hypothesized that AHR might suppress LPA-induced VEGF-A expression in PC-3 cells by competing with HIF-1alpha for ARNT. lysophosphatidic acid 107-110 hypoxia inducible factor 1 subunit alpha Homo sapiens 55-65 23831623-5 2013 Since ARNT is a common dimerization partner of AHR and HIF-1alpha, we hypothesized that AHR might suppress LPA-induced VEGF-A expression in PC-3 cells by competing with HIF-1alpha for ARNT. lysophosphatidic acid 107-110 hypoxia inducible factor 1 subunit alpha Homo sapiens 169-179 23831623-6 2013 Here we demonstrated that overexpression and ligand activation of AHR inhibited HIF-1-mediated VEGF-A induction by LPA treatment of PC-3 cells. lysophosphatidic acid 115-118 hypoxia inducible factor 1 subunit alpha Homo sapiens 80-85 28236660-11 2017 Furthermore, the bioenergetic status of LPA-treated ovarian cancer cells mimics hypoxia through induction of hypoxia-inducible factor-1alpha, which was found to transactivate ets-1. lysophosphatidic acid 40-43 hypoxia inducible factor 1 subunit alpha Homo sapiens 109-140 20724530-10 2010 In vitro studies using HCT116 cells showed that LPA induced cyclin D1, c-Myc, and HIF-1alpha expression, which was attenuated by knockdown of LPA(2). lysophosphatidic acid 48-51 hypoxia inducible factor 1 subunit alpha Homo sapiens 82-92 20724530-10 2010 In vitro studies using HCT116 cells showed that LPA induced cyclin D1, c-Myc, and HIF-1alpha expression, which was attenuated by knockdown of LPA(2). lysophosphatidic acid 142-145 hypoxia inducible factor 1 subunit alpha Homo sapiens 82-92 17919812-0 2007 Hypoxia enhances LPA-induced HIF-1alpha and VEGF expression: their inhibition by resveratrol. lysophosphatidic acid 17-20 hypoxia inducible factor 1 subunit alpha Homo sapiens 29-39 17919812-6 2007 Collectively, these results show that LPA under hypoxic condition enhances cell migration through the sequential induction of HIF-1alpha and VEGF, and that this enhancement is efficiently blocked by resveratrol. lysophosphatidic acid 38-41 hypoxia inducible factor 1 subunit alpha Homo sapiens 126-136 16912173-7 2006 LPA-induced cell migration required cytosolic phospholipase A(2) (cPLA(2)) and LPA stimulates cPLA(2) phosphorylation in a HIF1 alpha-dependent manner under hypoxia conditions. lysophosphatidic acid 0-3 hypoxia inducible factor 1 subunit alpha Homo sapiens 123-133 16912173-7 2006 LPA-induced cell migration required cytosolic phospholipase A(2) (cPLA(2)) and LPA stimulates cPLA(2) phosphorylation in a HIF1 alpha-dependent manner under hypoxia conditions. lysophosphatidic acid 79-82 hypoxia inducible factor 1 subunit alpha Homo sapiens 123-133 16912173-11 2006 HIF1 alpha plays a critical role in enhancing and/or sensitizing the role of LPA on cell migration and invasion under hypoxic conditions, where cPLA(2) is required for LPA-induced cell migration. lysophosphatidic acid 77-80 hypoxia inducible factor 1 subunit alpha Homo sapiens 0-10 16912173-11 2006 HIF1 alpha plays a critical role in enhancing and/or sensitizing the role of LPA on cell migration and invasion under hypoxic conditions, where cPLA(2) is required for LPA-induced cell migration. lysophosphatidic acid 168-171 hypoxia inducible factor 1 subunit alpha Homo sapiens 0-10 34065317-7 2021 Moreover, we observed that HIF-1alpha, located downstream of the mTOR, is implicated in LPA-induced DDR2 expression and ovarian cancer cell invasion. lysophosphatidic acid 88-91 hypoxia inducible factor 1 subunit alpha Homo sapiens 27-37 34065317-8 2021 Finally, we provide evidence that LPA-induced HIF-1alpha expression mediates Twist1 expression to upregulate DDR2 expression. lysophosphatidic acid 34-37 hypoxia inducible factor 1 subunit alpha Homo sapiens 46-56 18802925-0 2008 Lysophosphatidic acid activates telomerase in ovarian cancer cells through hypoxia-inducible factor-1alpha and the PI3K pathway. lysophosphatidic acid 0-21 hypoxia inducible factor 1 subunit alpha Homo sapiens 75-106 17085645-0 2006 Activation of hypoxia-inducible factor-1alpha is necessary for lysophosphatidic acid-induced vascular endothelial growth factor expression. lysophosphatidic acid 63-84 hypoxia inducible factor 1 subunit alpha Homo sapiens 14-45 17085645-3 2006 In the present study, we investigated whether LPA activates hypoxia inducible factor-1 (HIF-1), a key transcriptional complex in tumor progression and metastasis, thereby increasing the expression of VEGF. lysophosphatidic acid 46-49 hypoxia inducible factor 1 subunit alpha Homo sapiens 60-86 17085645-3 2006 In the present study, we investigated whether LPA activates hypoxia inducible factor-1 (HIF-1), a key transcriptional complex in tumor progression and metastasis, thereby increasing the expression of VEGF. lysophosphatidic acid 46-49 hypoxia inducible factor 1 subunit alpha Homo sapiens 88-93 17085645-6 2006 RESULTS: LPA induced expressions of VEGF and HIF-1alpha in OVCAR-3, CAOV-3, PC-3, and SK-Hep1 cells but not in SKOV-3 and Hep-3B cells. lysophosphatidic acid 9-12 hypoxia inducible factor 1 subunit alpha Homo sapiens 45-55 17085645-7 2006 In OVCAR-3 and PC-3 cells, the phosphoinositide 3-kinase/Akt/mammalian target of rapamycin/p70S6K and p42/p44 mitogen-activated protein kinase pathways were required for LPA-induced HIF-1alpha and VEGF expressions, whereas only the phosphoinositide 3-kinase/mammalian target of rapamycin/p70S6K pathway was important in SK-Hep1 cells. lysophosphatidic acid 170-173 hypoxia inducible factor 1 subunit alpha Homo sapiens 182-192 17085645-8 2006 Immunofluorescence microscopy assay showed translocation of HIF-1alpha to nucleus by LPA, and chromatin immunoprecipitation assay revealed the binding of HIF-1alpha to the promoter of VEGF by LPA. lysophosphatidic acid 85-88 hypoxia inducible factor 1 subunit alpha Homo sapiens 60-70 17085645-8 2006 Immunofluorescence microscopy assay showed translocation of HIF-1alpha to nucleus by LPA, and chromatin immunoprecipitation assay revealed the binding of HIF-1alpha to the promoter of VEGF by LPA. lysophosphatidic acid 192-195 hypoxia inducible factor 1 subunit alpha Homo sapiens 154-164 17085645-10 2006 CONCLUSIONS: Our results show for the first time that LPA induces VEGF via HIF-1alpha activation and reveal a critical role of HIF-1alpha in LPA-induced cancer cell proliferation and angiogenesis. lysophosphatidic acid 54-57 hypoxia inducible factor 1 subunit alpha Homo sapiens 75-85 17085645-10 2006 CONCLUSIONS: Our results show for the first time that LPA induces VEGF via HIF-1alpha activation and reveal a critical role of HIF-1alpha in LPA-induced cancer cell proliferation and angiogenesis. lysophosphatidic acid 141-144 hypoxia inducible factor 1 subunit alpha Homo sapiens 127-137 31867678-7 2020 Observations of this investigation suggest that LPA supports survival of T lymphoma cells via altering apoptosis and glucose metabolism through changing the level of reactive species, namely nitric oxide and reactive oxygen species along with expression of various survival and glucose metabolism regulatory molecules, including hypoxia-inducible factor 1-alpha, p53, Bcl2, and glucose transporter 3, hexokinase II, pyruvate kinase muscle isozyme 2, monocarboxylate transporter 1, pyruvate dehydrogenase kinase 1. lysophosphatidic acid 48-51 hypoxia inducible factor 1 subunit alpha Homo sapiens 329-361 29386184-4 2018 LPA stimulated a pseudohypoxic response via Rac-mediated activation of NADPH oxidase and generation of reactive oxygen species, resulting in activation of HIF1alpha. lysophosphatidic acid 0-3 hypoxia inducible factor 1 subunit alpha Homo sapiens 155-164 29386184-7 2018 These studies reveal a critical role for LPA in metabolic reprogramming of ovarian cancer cells and identify this node as a promising therapeutic target in ovarian cancer.Significance: These findings establish LPA as a potential therapeutic target in ovarian cancer, revealing its role in the activation of HIF1alpha-mediated metabolic reprogramming in this disease. lysophosphatidic acid 210-213 hypoxia inducible factor 1 subunit alpha Homo sapiens 307-316 26352431-0 2015 HIF1alpha-Induced by Lysophosphatidic Acid Is Stabilized via Interaction with MIF and CSN5. lysophosphatidic acid 21-42 hypoxia inducible factor 1 subunit alpha Homo sapiens 0-9 26352431-8 2015 We have shown recently that LPA increases hypoxia-inducible factor 1alpha (HIF1alpha) expression. lysophosphatidic acid 28-31 hypoxia inducible factor 1 subunit alpha Homo sapiens 75-84 26352431-9 2015 We found that MIF regulation by LPA was ablated by knockdown of HIF1alpha, indicating that MIF is a transcriptional target of HIF1alpha. lysophosphatidic acid 32-35 hypoxia inducible factor 1 subunit alpha Homo sapiens 64-73 26352431-9 2015 We found that MIF regulation by LPA was ablated by knockdown of HIF1alpha, indicating that MIF is a transcriptional target of HIF1alpha. lysophosphatidic acid 32-35 hypoxia inducible factor 1 subunit alpha Homo sapiens 126-135 26352431-10 2015 Conversely, knockdown of MIF ablated an increase in HIF1alpha expression in LPA-treated cells, suggesting a reciprocal relationship between HIF1alpha and MIF. lysophosphatidic acid 76-79 hypoxia inducible factor 1 subunit alpha Homo sapiens 52-61 26352431-10 2015 Conversely, knockdown of MIF ablated an increase in HIF1alpha expression in LPA-treated cells, suggesting a reciprocal relationship between HIF1alpha and MIF. lysophosphatidic acid 76-79 hypoxia inducible factor 1 subunit alpha Homo sapiens 140-149 26352431-11 2015 LPA stimulated co-immunoprecipitation of HIF1alpha and MIF, indicating that their association is necessary for stabilization of HIF1alpha. lysophosphatidic acid 0-3 hypoxia inducible factor 1 subunit alpha Homo sapiens 41-50 26352431-11 2015 LPA stimulated co-immunoprecipitation of HIF1alpha and MIF, indicating that their association is necessary for stabilization of HIF1alpha. lysophosphatidic acid 0-3 hypoxia inducible factor 1 subunit alpha Homo sapiens 128-137 26352431-13 2015 We found that LPA did not alter expression of CSN5, but stimulated its interaction with HIF1alpha and MIF. lysophosphatidic acid 14-17 hypoxia inducible factor 1 subunit alpha Homo sapiens 88-97 27166196-0 2016 Lysophosphatidic acid stimulates epithelial to mesenchymal transition marker Slug/Snail2 in ovarian cancer cells via Galphai2, Src, and HIF1alpha signaling nexus. lysophosphatidic acid 0-21 hypoxia inducible factor 1 subunit alpha Homo sapiens 136-145 27166196-2 2016 Using a transcription factor activation reporter array, which analyzes 45 distinct transcription factors, it has been observed that LPA observed robustly activates the transcription factor hypoxia-induced factor-1alpha (HIF1alpha) in SKOV3.ip ovarian cancer cells. lysophosphatidic acid 132-135 hypoxia inducible factor 1 subunit alpha Homo sapiens 220-229 27166196-5 2016 Our report demonstrates that LPA stimulates the increase in HIF1alpha levels via Galphai2. lysophosphatidic acid 29-32 hypoxia inducible factor 1 subunit alpha Homo sapiens 60-69 27166196-8 2016 In line with the established role of EMT in promoting invasive cell migration, our data demonstrates that the inhibition of HIF1alpha with the clinically used HIF1alpha inhibitor, PX-478, drastically attenuates LPA-stimulates invasive migration of SKOV3.ip cells. lysophosphatidic acid 211-214 hypoxia inducible factor 1 subunit alpha Homo sapiens 124-133 27166196-8 2016 In line with the established role of EMT in promoting invasive cell migration, our data demonstrates that the inhibition of HIF1alpha with the clinically used HIF1alpha inhibitor, PX-478, drastically attenuates LPA-stimulates invasive migration of SKOV3.ip cells. lysophosphatidic acid 211-214 hypoxia inducible factor 1 subunit alpha Homo sapiens 159-168 27166196-9 2016 Thus, our present study demonstrates that LPA utilizes a Galphai2-mediated signaling pathway via Src kinase to stimulate an increase in HIF1alpha levels and downstream EMT-specific factors such as Slug, leading to invasive migration of ovarian cancer cells. lysophosphatidic acid 42-45 hypoxia inducible factor 1 subunit alpha Homo sapiens 136-145