PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 21708150-2 2011 The N-terminus of the beta2-adrenergic receptor expressed on the cell surface was doubly labeled with pH-sensitive fluorescein and pH-insensitive tetramethylrhodamine. Fluorescein 115-126 adrenoceptor beta 2 Homo sapiens 22-47 11353823-2 2001 To monitor directly the formation of the active state of a prototypical GPCR, we devised a method to site specifically attach fluorescein to an endogenous cysteine (Cys-265) at the cytoplasmic end of transmembrane 6 (TM6) of the beta(2) adrenergic receptor (beta(2)AR), adjacent to the G-protein-coupling domain. Fluorescein 126-137 adrenoceptor beta 2 Homo sapiens 229-256 11353823-2 2001 To monitor directly the formation of the active state of a prototypical GPCR, we devised a method to site specifically attach fluorescein to an endogenous cysteine (Cys-265) at the cytoplasmic end of transmembrane 6 (TM6) of the beta(2) adrenergic receptor (beta(2)AR), adjacent to the G-protein-coupling domain. Fluorescein 126-137 adrenoceptor beta 2 Homo sapiens 258-267 32435777-2 2020 Herein, we reported a site-specifically labelled small-sized fluorescent pair 7-HC/FlAsH ((7-hydroxycoumarin-4-yl)-ethylglycine/fluorescein arsenical hairpin) for fluorescence lifetime based FRET (fluorescence resonance energy transfer) to reveal conformational differences of Galphai1 (inhibitory G proteins) and Galphas (stimulatory G proteins) upon beta2AR (beta2-adrenergic receptor) coupling. Fluorescein 128-139 adrenoceptor beta 2 Homo sapiens 361-386 28891646-10 2017 The suitability of this system in the cell-surface labeling of live cells was demonstrated using a SNAP-tag approach to install the boronic acid reagent onto the extracellular domain of the Beta-2 adrenergic receptor in HEK293T cells, followed by incubation with the optimal thiosemicarbazide-functionalized nopoldiol reagent labeled with fluorescein dye. Fluorescein 339-350 adrenoceptor beta 2 Homo sapiens 190-216