PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 28528119-7 2017 Specifically, the mTOR-dependent autophagy inducer rapamycin enhanced the knockdown efficiency of both lipoplex and polyplex, whereas mTOR-dependent autophagy inhibitor 3-methyladenine (3-MA) suppressed their silencing efficiency. 3-methyladenine 169-184 mechanistic target of rapamycin kinase Homo sapiens 134-138 28528119-7 2017 Specifically, the mTOR-dependent autophagy inducer rapamycin enhanced the knockdown efficiency of both lipoplex and polyplex, whereas mTOR-dependent autophagy inhibitor 3-methyladenine (3-MA) suppressed their silencing efficiency. 3-methyladenine 186-190 mechanistic target of rapamycin kinase Homo sapiens 134-138 28528119-16 2017 Specifically, the mTOR-dependent autophagy inducer rapamycin enhances the knockdown efficiency of both lipoplex and polyplex, whereas mTOR-dependent autophagy inhibitor 3-methyladenine suppresses their silencing efficiency. 3-methyladenine 169-184 mechanistic target of rapamycin kinase Homo sapiens 134-138 24890505-4 2014 Using 3-methyladenine (3-MA) and rapamycin to regulate the activity of mTOR, it was found that the efficiency of neuronal differentiation was affected. 3-methyladenine 6-21 mechanistic target of rapamycin kinase Homo sapiens 71-75 27779641-6 2016 Furthermore, pretreatment with the autophagy inhibitor chloroquine or 3-methyladenine showed the potential in attenuating the apoptosis rate induced by Sal B. Mechanistically, Sal B treatment inhibited the AKT/mTOR signaling cascade in vitro. 3-methyladenine 70-85 mechanistic target of rapamycin kinase Homo sapiens 210-214 24890505-4 2014 Using 3-methyladenine (3-MA) and rapamycin to regulate the activity of mTOR, it was found that the efficiency of neuronal differentiation was affected. 3-methyladenine 23-27 mechanistic target of rapamycin kinase Homo sapiens 71-75 24319109-9 2014 Although rapamycin treatment induced autophagy and led to apoptosis promotion, the pro-apoptotic effect of rapamycin was reversed by the addition of 3-MA, suggesting that mTOR inhibition promotes endometriotic cell apoptosis via autophagy induction. 3-methyladenine 149-153 mechanistic target of rapamycin kinase Homo sapiens 171-175 23813779-5 2014 Conversely, autophagy inhibitor 3-methyladenine partly reversed the cytotoxicity and the phosphorylation of AMPK, mTOR and p70 S6K induced by Tan IIA in KBM-5 leukemia cells. 3-methyladenine 32-47 mechanistic target of rapamycin kinase Homo sapiens 114-118 23794518-10 2013 The addition of 3-MA could attenuate the inhibitory role on HER2/AKT/mTOR pathway and the active role on AMPK that was raised by lapatinib. 3-methyladenine 16-20 mechanistic target of rapamycin kinase Homo sapiens 69-73 35341775-12 2022 Treatment with the autophagy inhibitor 3-methyladenine (3-MA) attenuated the effect of metformin on apoptosis, autophagy, and the AMPK/mTOR/p70S6K signaling pathway. 3-methyladenine 39-54 mechanistic target of rapamycin kinase Homo sapiens 135-139 33777755-10 2021 The AKT activator SC79 and autophagy inhibitor 3-methyladenine (3-MA) reversed the bupivacaine-inhibited phosphorylation of AKT and mTOR and bupivacaine-induced autophagy, as well as the bupivacaine-attenuated NSCLC progression in the cells. 3-methyladenine 47-62 mechanistic target of rapamycin kinase Homo sapiens 132-136 33777755-10 2021 The AKT activator SC79 and autophagy inhibitor 3-methyladenine (3-MA) reversed the bupivacaine-inhibited phosphorylation of AKT and mTOR and bupivacaine-induced autophagy, as well as the bupivacaine-attenuated NSCLC progression in the cells. 3-methyladenine 64-68 mechanistic target of rapamycin kinase Homo sapiens 132-136 34734026-9 2021 And the autophagy-related pathway protein p-AMPKalpha was increased and p-mTOR was reduced by 3-MA treatment. 3-methyladenine 94-98 mechanistic target of rapamycin kinase Homo sapiens 74-78 34484820-13 2021 The miR-149-induced protection against HUVECs injury could be reversed by cotreatment with 3-methyladenine (3-MA, an autophagy inhibitor) or insulin (an activator of Akt/mTOR pathway). 3-methyladenine 91-106 mechanistic target of rapamycin kinase Homo sapiens 170-174 34484820-13 2021 The miR-149-induced protection against HUVECs injury could be reversed by cotreatment with 3-methyladenine (3-MA, an autophagy inhibitor) or insulin (an activator of Akt/mTOR pathway). 3-methyladenine 108-112 mechanistic target of rapamycin kinase Homo sapiens 170-174 35341775-12 2022 Treatment with the autophagy inhibitor 3-methyladenine (3-MA) attenuated the effect of metformin on apoptosis, autophagy, and the AMPK/mTOR/p70S6K signaling pathway. 3-methyladenine 56-60 mechanistic target of rapamycin kinase Homo sapiens 135-139 31931659-12 2020 Collectively, our results identify ROS as central inducers of MTORC2 activation during chronic autophagy, which in turn fuels senescence activation and myofibroblast differentiation in distinct cellular subpopulations.Abbreviations: 3-MA: 3-methyladenine; ACTA2: actin, alpha 2, smooth muscle, aorta; AKT1: AKT serine/threonine kinase 1; p-AKT1: AKT1 Ser473 phosphorylation; t-AKT1: total AKT serine/threonine kinase 1; ATG4A: autophagy related 4A cysteine peptidase; ATG7: autophagy gene 7; C12FDG: 5-dodecanoylaminofluorescein Di-beta-D-Galactopyranoside; CDKN1A: cyclin dependent kinase inhibitor 1A; CDKN2A: cyclin dependent kinase inhibitor 2A; Ctl: control; DAPI: 4",6-diamidino-2-phenylindole, dilactate; ECM: extracellular matrix; GSH: L-glutathione reduced; H2O2: hydrogen peroxide; HLF: adult human lung fibroblasts; Ho: Hoechst 33342 (2"-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2.5"-bi-1H-benzimidazole); HSC: hepatic stellate cells; LY: LY294002; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MTORC1/2: mechanistic target of rapamycin kinase complex 1/2; N: normal growth medium; NAC: N-acetyl-L-cysteine; PBS: phosphate-buffered saline; PDGFA: platelet derived growth factor subunit A; PRKCA/PKCalpha: protein kinase C alpha; PtdIns3K: class III phosphatidylinositol 3-kinase; PTEN: phosphatase and tensin homolog; R: rapamycin; RICTOR: RPTOR independent companion of MTOR complex 2; ROS: reactive oxygen species; RPTOR: regulatory associated protein of MTOR complex 1; SA-GLB1/beta-gal: senescence-associated galactosidase beta 1; SGK1: serum/glucocorticoid regulated kinase 1; shRNA: short hairpin RNA; siCtl: control siRNA; siRNA: small interfering RNA; SQSTM1: sequestosome 1; SS: serum-free (serum starvation) medium; TP53: tumor protein p53; TUBA: tubulin alpha; V: vehicle. 3-methyladenine 239-254 mechanistic target of rapamycin kinase Homo sapiens 62-66 33978839-11 2021 Apatinib inhibited the PI3K/Akt/mTOR pathway, while 3-MA and the combination of 3-MA and apatinib significantly activated the PI3K/Akt/mTOR pathway and inhibited autophagy. 3-methyladenine 52-56 mechanistic target of rapamycin kinase Homo sapiens 135-139 33978839-11 2021 Apatinib inhibited the PI3K/Akt/mTOR pathway, while 3-MA and the combination of 3-MA and apatinib significantly activated the PI3K/Akt/mTOR pathway and inhibited autophagy. 3-methyladenine 80-84 mechanistic target of rapamycin kinase Homo sapiens 135-139 32319603-7 2020 The downregulation of p-mTOR expression by ADAM12 silencing was rescued in 3-methyladenine-treated JEG-3 cells, indicating that mTOR might participate in the autophagy-mediated pro-apoptotic effect of ADAM12 silencing. 3-methyladenine 75-90 mechanistic target of rapamycin kinase Homo sapiens 24-28 32691991-8 2020 Co-administration of 3-MA with Puerarin 6""-O-xyloside obviously augmented these effects including inhibited protein expression of p-PI3K, p-AKT, and p-mTOR, and inhibited proliferation, promoted apoptosis, and decreased stemness. 3-methyladenine 21-25 mechanistic target of rapamycin kinase Homo sapiens 152-156 32319603-7 2020 The downregulation of p-mTOR expression by ADAM12 silencing was rescued in 3-methyladenine-treated JEG-3 cells, indicating that mTOR might participate in the autophagy-mediated pro-apoptotic effect of ADAM12 silencing. 3-methyladenine 75-90 mechanistic target of rapamycin kinase Homo sapiens 128-132 31696568-6 2020 In contrast, inactivation of autophagy by 3-methyladenine or Bafilomycin A1 could attenuate OPG-mediated inhibition of OC differentiation via the AMPK/mTOR/p70S6K signalling pathway. 3-methyladenine 42-57 mechanistic target of rapamycin kinase Homo sapiens 151-155 31497240-4 2019 Treatment with autophagic inhibitor 3-MA, Baf-A1 and PI3K agonist, IGF-1, fully proved our conclusion, as well as uncovered the potential downregulated signaling pathway, PI3K/AKT/mTOR. 3-methyladenine 36-40 mechanistic target of rapamycin kinase Homo sapiens 180-184 31277013-11 2019 On the contrary, pretreatment with 3-methyladenine significantly attenuated intestinal injury and autophagy level and upregulated expression of p-mTOR/mTOR. 3-methyladenine 35-50 mechanistic target of rapamycin kinase Homo sapiens 146-150 31277013-11 2019 On the contrary, pretreatment with 3-methyladenine significantly attenuated intestinal injury and autophagy level and upregulated expression of p-mTOR/mTOR. 3-methyladenine 35-50 mechanistic target of rapamycin kinase Homo sapiens 151-155 30170968-5 2018 In vivo modulation of mTOR and autophagy was achieved by using AZD8055, rapamycin and 3-methyladenine. 3-methyladenine 86-101 mechanistic target of rapamycin kinase Homo sapiens 22-26 30897708-7 2019 CLE-10 that was pretreated with 3-methyladenine (3-MA) or chloroquine (CQ) weakened the upregulation of the protein expression of p-ULK1, or the downregulation of p62, p-mTOR, and decreased the level of cytotoxicity against MDA-MB-231 cells. 3-methyladenine 32-47 mechanistic target of rapamycin kinase Homo sapiens 170-174 30897708-7 2019 CLE-10 that was pretreated with 3-methyladenine (3-MA) or chloroquine (CQ) weakened the upregulation of the protein expression of p-ULK1, or the downregulation of p62, p-mTOR, and decreased the level of cytotoxicity against MDA-MB-231 cells. 3-methyladenine 49-53 mechanistic target of rapamycin kinase Homo sapiens 170-174 30416863-13 2018 The inhibitory effect of URI silencing on mTOR and p70S6K phosphorylation was antagonized by the autophagy inhibitor 3-methyladenine. 3-methyladenine 117-132 mechanistic target of rapamycin kinase Homo sapiens 42-46