PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 23595107-1 2013 Positively charged gold nanoparticles can effectively differentiate ATP and ADP, thus providing a simple and visual approach to colorimetric detection of hexokinase activity and inhibition. Adenosine Diphosphate 76-79 hexokinase 1 Homo sapiens 154-164 22322891-12 2012 These results suggest that mitochondrially bound HK supporting the ADP/ATP exchange activity levels facilitates the 3-BrPA inhibition reaction in tumors mitochondria by a proton motive force-dependent dynamic equilibrium between sensitive and less sensitive SDH in the electron transport system. Adenosine Diphosphate 67-70 hexokinase 1 Homo sapiens 49-51 19306267-0 2009 Hexokinase inhibitor screening based on adenosine 5"-diphosphate determination by electrophoretically mediated microanalysis. Adenosine Diphosphate 40-64 hexokinase 1 Homo sapiens 0-10 19306267-2 2009 In this method, hexokinase activity was assayed via electrophoretically mediated microanalysis (EMMA), which combines on-column hexokinase-mediated reaction and measurement of produced adenosine 5"-diphosphate (ADP) via electrophoretical separation and UV detection. Adenosine Diphosphate 185-209 hexokinase 1 Homo sapiens 16-26 19306267-2 2009 In this method, hexokinase activity was assayed via electrophoretically mediated microanalysis (EMMA), which combines on-column hexokinase-mediated reaction and measurement of produced adenosine 5"-diphosphate (ADP) via electrophoretical separation and UV detection. Adenosine Diphosphate 211-214 hexokinase 1 Homo sapiens 16-26 15752705-2 2005 Because glucokinase has been resistant to crystallization, computer generated homology models were developed based on the X-ray crystal structure of the COOH-terminal domain of human brain hexokinase 1 bound to glucose and ADP or glucose and glucose-6-phosphate. Adenosine Diphosphate 223-226 hexokinase 1 Homo sapiens 189-201 18324728-4 2008 This is achieved by coupling the formation of ATP, as a consequence of gamma-phosphate transfer from NTP to ADP, to hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PDH), oxidized nicotinamide adenine dinucleotide phosphate (NADP), phenazine methosulfate (PMS), and iodonitrotetrazolium chloride (INT). Adenosine Diphosphate 108-111 hexokinase 1 Homo sapiens 116-126 18324728-4 2008 This is achieved by coupling the formation of ATP, as a consequence of gamma-phosphate transfer from NTP to ADP, to hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PDH), oxidized nicotinamide adenine dinucleotide phosphate (NADP), phenazine methosulfate (PMS), and iodonitrotetrazolium chloride (INT). Adenosine Diphosphate 108-111 hexokinase 1 Homo sapiens 128-130 15189857-4 2004 The model is illustrated by the quantification of nanomolar levels of ADP (and/or ATP) in a continuous assay involving the enzymes L-lactate dehydrogenase and L-lactate oxidase to cycle the pyruvate accumulated in a first enzymatic cycle constituted by the enzymes pyruvate kinase and hexokinase. Adenosine Diphosphate 70-73 hexokinase 1 Homo sapiens 285-295 11121265-5 2001 HK activity was obtained by measuring ATP and ADP amounts with HPLC. Adenosine Diphosphate 46-49 hexokinase 1 Homo sapiens 0-2 15065809-2 2004 The enzymatic conversion of nucleotide adenosine triphosphate (ATP) to adenosine diphosphate (ADP) by hexokinase (HK) was monitored in the bioreactor interfaced by a laboratory-built microsampler to a capillary electrophoresis unit. Adenosine Diphosphate 71-92 hexokinase 1 Homo sapiens 102-112 15065809-2 2004 The enzymatic conversion of nucleotide adenosine triphosphate (ATP) to adenosine diphosphate (ADP) by hexokinase (HK) was monitored in the bioreactor interfaced by a laboratory-built microsampler to a capillary electrophoresis unit. Adenosine Diphosphate 71-92 hexokinase 1 Homo sapiens 114-116 15065809-2 2004 The enzymatic conversion of nucleotide adenosine triphosphate (ATP) to adenosine diphosphate (ADP) by hexokinase (HK) was monitored in the bioreactor interfaced by a laboratory-built microsampler to a capillary electrophoresis unit. Adenosine Diphosphate 94-97 hexokinase 1 Homo sapiens 102-112 15065809-2 2004 The enzymatic conversion of nucleotide adenosine triphosphate (ATP) to adenosine diphosphate (ADP) by hexokinase (HK) was monitored in the bioreactor interfaced by a laboratory-built microsampler to a capillary electrophoresis unit. Adenosine Diphosphate 94-97 hexokinase 1 Homo sapiens 114-116 12458196-11 2003 4) In contrast, the hexokinase-treated poorly hydrolyzable ATP analogue, adenosine 5"-O-(thiotriphosphate) (ATPgammaS), enhances ADP trapping to a similar extent as ATP under conditions in which ATPgammaS should not be hydrolyzed. Adenosine Diphosphate 129-132 hexokinase 1 Homo sapiens 20-30 10686099-0 2000 Crystal structures of mutant monomeric hexokinase I reveal multiple ADP binding sites and conformational changes relevant to allosteric regulation. Adenosine Diphosphate 68-71 hexokinase 1 Homo sapiens 39-51 10387081-5 1999 Small-angle X-ray scattering data from the mutant hexokinase I in the presence of glucose/phosphate, glucose/glucose 6-phosphate, and glucose/ADP/Mg2+/AlF3 are consistent with a rodlike conformation for the monomer similar to that observed in crystal structures of the hexokinase I dimer. Adenosine Diphosphate 142-145 hexokinase 1 Homo sapiens 50-62 10574795-4 1999 The mitochondrion-bound hexokinase I is believed to optimize the ATP/ADP exchange between glucose phosphorylation and the mitochondrial oxidative phosphorylation reactions. Adenosine Diphosphate 69-72 hexokinase 1 Homo sapiens 24-36 3207715-4 1988 Using three methods to determine the extramitochondrial ATP/ADP ratio, we observed that at high ATP/ADP ratios the relationship between respiratory rate and log (ATP/ADP) deviated in a sigmoidal fashion from linearity, if the amount of hexokinase present was modulated. Adenosine Diphosphate 60-63 hexokinase 1 Homo sapiens 236-246 9886057-2 1999 The vesicles derived from the cerebellum were able to accumulate Ca2+ in a medium containing ADP when either glucose 6-phosphate and hexokinase or fructose 1,6-bisphosphate and phosphofructokinase were added to the medium. Adenosine Diphosphate 93-96 hexokinase 1 Homo sapiens 102-143 7683206-10 1993 The results suggest three things: (i) that contact sites are probably more frequent in the intact cell than in vitro in the absence of macromolecules, (ii) that the contact preference of hexokinase serves rather the ADP supply of the translocator than the ATP transfer to the enzyme and (iii) that the total cellular hexokinase activity may be regulated by specific binding of the enzyme to the contact sites, either because of a different pore structure or because of additional components exclusively exposed in these sites. Adenosine Diphosphate 216-219 hexokinase 1 Homo sapiens 187-197 7683206-10 1993 The results suggest three things: (i) that contact sites are probably more frequent in the intact cell than in vitro in the absence of macromolecules, (ii) that the contact preference of hexokinase serves rather the ADP supply of the translocator than the ATP transfer to the enzyme and (iii) that the total cellular hexokinase activity may be regulated by specific binding of the enzyme to the contact sites, either because of a different pore structure or because of additional components exclusively exposed in these sites. Adenosine Diphosphate 216-219 hexokinase 1 Homo sapiens 317-327 1309800-1 1992 In the presence of hexokinase, vesicles derived from the sarcoplasmic reticulum of skeletal muscle are able to accumulate Ca2+ in a medium containing ADP and glucose 6-phosphate. Adenosine Diphosphate 150-153 hexokinase 1 Homo sapiens 19-29 2140894-2 1990 The involvement of dansylated ATP, ADP and AMP as substrate analogues in energy metabolism is demonstrated in the ATPase, hexokinase, pyruvate kinase and adenylate kinase reactions. Adenosine Diphosphate 35-38 hexokinase 1 Homo sapiens 122-132 34623563-3 2022 Hexokinase (HK) is a key enzyme on glucose metabolism and is coupled to the brain mitochondrial redox modulation by recycling ADP for oxidative phosphorylation (OXPHOS). Adenosine Diphosphate 126-129 hexokinase 1 Homo sapiens 0-10 34623563-3 2022 Hexokinase (HK) is a key enzyme on glucose metabolism and is coupled to the brain mitochondrial redox modulation by recycling ADP for oxidative phosphorylation (OXPHOS). Adenosine Diphosphate 126-129 hexokinase 1 Homo sapiens 12-14 10222010-5 1999 Here, we present a simple photometric assay that uses a cyclic detection system which, due to the sequential action of pyruvate kinase and hexokinase, results in an exponential increase of ADP and glucose 6-phosphate. Adenosine Diphosphate 189-192 hexokinase 1 Homo sapiens 139-149 9387093-2 1997 Drawing on the hexokinase-mitochondrial acceptor theory of insulin action, this article presents evidence suggesting that the increased binding of hexokinase to porin on mitochondria of cancer cells not only accelerates glycolysis by providing hexokinase with better access to ATP, but also stimulates the TCA cycle by providing the mitochondrion with ADP that acts as an acceptor for phosphoryl groups. Adenosine Diphosphate 352-355 hexokinase 1 Homo sapiens 147-157 9387093-2 1997 Drawing on the hexokinase-mitochondrial acceptor theory of insulin action, this article presents evidence suggesting that the increased binding of hexokinase to porin on mitochondria of cancer cells not only accelerates glycolysis by providing hexokinase with better access to ATP, but also stimulates the TCA cycle by providing the mitochondrion with ADP that acts as an acceptor for phosphoryl groups. Adenosine Diphosphate 352-355 hexokinase 1 Homo sapiens 147-157 34516596-6 2021 Specifically, we found that glucose oxidase and hexokinase fragment in the presence of D-glucose but not L-glucose, while hexokinase aggregates in the presence of Mg2+ ion and either ATP or ADP at low pH. Adenosine Diphosphate 190-193 hexokinase 1 Homo sapiens 48-58 34516596-6 2021 Specifically, we found that glucose oxidase and hexokinase fragment in the presence of D-glucose but not L-glucose, while hexokinase aggregates in the presence of Mg2+ ion and either ATP or ADP at low pH. Adenosine Diphosphate 190-193 hexokinase 1 Homo sapiens 122-132 2525930-8 1989 In many tissues, it has been shown that the binding of hexokinase to the mitochondrial outer membrane allows a preferential utilization of the ATP generated by oxidative phosphorylation which, in turn, is activated by immediate restitution of ADP. Adenosine Diphosphate 243-246 hexokinase 1 Homo sapiens 55-65 3207715-4 1988 Using three methods to determine the extramitochondrial ATP/ADP ratio, we observed that at high ATP/ADP ratios the relationship between respiratory rate and log (ATP/ADP) deviated in a sigmoidal fashion from linearity, if the amount of hexokinase present was modulated. Adenosine Diphosphate 100-103 hexokinase 1 Homo sapiens 236-246 3207715-4 1988 Using three methods to determine the extramitochondrial ATP/ADP ratio, we observed that at high ATP/ADP ratios the relationship between respiratory rate and log (ATP/ADP) deviated in a sigmoidal fashion from linearity, if the amount of hexokinase present was modulated. Adenosine Diphosphate 100-103 hexokinase 1 Homo sapiens 236-246 2990572-11 1985 Under conditions of our standard experiment ([ADP] = 0.5 mM), adenylate kinase provides about 50% of the ATP used by hexokinase in well-coupled mitochondria. Adenosine Diphosphate 46-49 hexokinase 1 Homo sapiens 117-127 3245387-4 1988 The stimulatory effect of mitochondria on glycolysis was associated with the decrease of adenylate energy charge which was caused by an apparently very fast production of ADP in the hexokinase reaction. Adenosine Diphosphate 171-174 hexokinase 1 Homo sapiens 182-192 3245387-6 1988 It is therefore suggested that the stimulatory effect of epithelioma mitochondria on glycolysis can be explained by production of ADP by the hexokinase associated with these mitochondria. Adenosine Diphosphate 130-133 hexokinase 1 Homo sapiens 141-151 2974916-8 1987 A twofold displacement of particulate hexokinase by ATP, ADP, 1-O-methylglucose, sorbitol-6-phosphate, and dibutyryl cyclic AMP was observed in the high-grade glioma-derived lines. Adenosine Diphosphate 57-60 hexokinase 1 Homo sapiens 38-48 3877250-10 1985 High energy phosphate compounds (ATP, ADP, CTP, and others) displaced mitochondria-bound hexokinase, which increased the cytosolic form by 2-fold in the glioma lines, but fibroblast hexokinase distribution was unaffected. Adenosine Diphosphate 38-41 hexokinase 1 Homo sapiens 89-99 3366651-4 1988 Next, exogenous and endogenous hexokinase catalyses the reaction between ATP and D-glucose to yield D-glucose-6-phosphate and ADP. Adenosine Diphosphate 126-129 hexokinase 1 Homo sapiens 31-41 3319615-11 1987 From this it appears that the pair of linked enzymes comprise a functional compartment supported by propinquity in which hexokinase has preferential access to ATP produced by creatine kinase, and creatine kinase to ADP from the hexokinase reaction. Adenosine Diphosphate 215-218 hexokinase 1 Homo sapiens 121-131 3319615-11 1987 From this it appears that the pair of linked enzymes comprise a functional compartment supported by propinquity in which hexokinase has preferential access to ATP produced by creatine kinase, and creatine kinase to ADP from the hexokinase reaction. Adenosine Diphosphate 215-218 hexokinase 1 Homo sapiens 228-238 2990572-5 1985 Addition of ADP to well-coupled mitochondria in the presence of an oxidizable substrate initiates the synthesis of glucose 6-phosphate via bound hexokinase. Adenosine Diphosphate 12-15 hexokinase 1 Homo sapiens 145-155 3856279-4 1985 Without inhibitors or in the presence of glucose 1,6-bisphosphate,2,3-diphosphoglycerate, and ADP, maximum hexokinase activity was observed at 5 mM glucose concentration. Adenosine Diphosphate 94-97 hexokinase 1 Homo sapiens 107-117 6736560-2 1984 In a subsequent incubation in the presence of pyruvate kinase, phosphoenolpyruvate, radioactive glucose and hexokinase, ATP and ADP are estimated together by coupling their recycling to the formation of glucose 6-phosphate. Adenosine Diphosphate 128-131 hexokinase 1 Homo sapiens 108-118 6540783-1 1984 Using hexokinase, glucose, and ATP to vary reversibly the concentrations of ADP and ATP in solution and bound to Acanthamoeba actin, I measured the relative critical concentrations and elongation rate constants for ATP-actin and ADP-actin in 50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 0.1 mM nucleotide, 0.1 mM CaCl2, 10 mM imidazole, pH 7. Adenosine Diphosphate 76-79 hexokinase 1 Homo sapiens 6-16 6416107-2 1983 Both procedures are based on the measurement of ADP formed during enzymatic phosphorylation of the analogs either by hexokinase or by fructokinase. Adenosine Diphosphate 48-51 hexokinase 1 Homo sapiens 117-127 6231056-4 1984 In the homogeneous control system composed of hexokinase and glucose as ATPase, soluble creatine kinase rapidly rephosphorylated ADP produced in the presence of 1 mM ATP, but the addition of pyruvate kinase in an increasing amount inhibited the reaction of creatine release from phosphocreatine and symmetrically increased the rate of pyruvate production from phosphoenol pyruvate. Adenosine Diphosphate 129-132 hexokinase 1 Homo sapiens 46-56 909782-1 1977 Hexokinase (EC 2.7.1.1) will convert commercially available alpha-[(32)P]-labelled ATP into alpha-[(32)P]-labelled ADP. Adenosine Diphosphate 115-118 hexokinase 1 Homo sapiens 0-10 6859536-2 1983 In the assay, production of ATP from carbamoyl phosphate and ADP by carbamate kinase is coupled to the formation of NADPH, using glucose, hexokinase, NADP+, and glucose-6-phosphate dehydrogenase. Adenosine Diphosphate 61-64 hexokinase 1 Homo sapiens 138-148 7426668-5 1980 Although it has been reported that the only effective nucleotide as the phosphoryl donor for hexokinase from various origin in ATP, and that ADP, a reaction product, inhibits the enzyme, hexokinase D from the rainbow-trout liver was found to be able to form glucose 6-phosphate (Glc-6-P) from glucose and various nucleotides such as ATP, ADP, CTP, GTP, UTP and UDP. Adenosine Diphosphate 141-144 hexokinase 1 Homo sapiens 93-103 7426668-5 1980 Although it has been reported that the only effective nucleotide as the phosphoryl donor for hexokinase from various origin in ATP, and that ADP, a reaction product, inhibits the enzyme, hexokinase D from the rainbow-trout liver was found to be able to form glucose 6-phosphate (Glc-6-P) from glucose and various nucleotides such as ATP, ADP, CTP, GTP, UTP and UDP. Adenosine Diphosphate 141-144 hexokinase 1 Homo sapiens 187-197 7426668-5 1980 Although it has been reported that the only effective nucleotide as the phosphoryl donor for hexokinase from various origin in ATP, and that ADP, a reaction product, inhibits the enzyme, hexokinase D from the rainbow-trout liver was found to be able to form glucose 6-phosphate (Glc-6-P) from glucose and various nucleotides such as ATP, ADP, CTP, GTP, UTP and UDP. Adenosine Diphosphate 338-341 hexokinase 1 Homo sapiens 187-197 215408-6 1978 The activities of the ADP derivatives, V, VIII, X, XIII, dextran-bound VIII, and dextran-bound XIII against acetate kinase were 82%, 81%, 68%, 55%, 35%, and 15%, respectively, relative to ADP and those of the ATP derivatives, VI, IX, XI, XIV, dextran-bound IX, and dextran-bound XIV against hexokinase were 88%, 94%, 60%, 81%, 58%, and 49%, respectively, relative to ATP. Adenosine Diphosphate 22-25 hexokinase 1 Homo sapiens 291-301 196856-3 1977 Coenzymic activities of the ADP analogue against acetate kinase and pyruvate kinase were 82% and 20%, respectively, relative to ADP and those of the ATP analogue against hexokinase and glycerokinase were 63% and 87%, respectively, relative to ATP. Adenosine Diphosphate 28-31 hexokinase 1 Homo sapiens 170-180 13143054-0 1954 The inhibition of brain hexokinase by adenosinediphosphate and sulfhydryl reagents. Adenosine Diphosphate 38-58 hexokinase 1 Homo sapiens 24-34 1140197-6 1975 The latter ATP analogue, when bound to Sepharose through its terminal amino group, could be dephosphorylated to the corresponding ADP analogue with soluble hexokinase yielding glucose 6-phosphate in an enzymic "solidphase" fashion. Adenosine Diphosphate 130-133 hexokinase 1 Homo sapiens 156-166 5289886-5 1971 In the presence of hexokinase and glucose, as well as arsenate and Mg(++), ADP inhibited H(+) uptake. Adenosine Diphosphate 75-78 hexokinase 1 Homo sapiens 19-29 5822062-2 1969 The kinetics of inhibition of brain soluble cytoplasmic hexokinase by ADP were examined in relation to variations in the concentrations of Mg(2+) and ATP. Adenosine Diphosphate 70-73 hexokinase 1 Homo sapiens 56-66 29119535-3 2018 Hexokinase (HK) is a key enzyme involved in glucose metabolism that modulates the level of brain mitochondrial ROS by recycling ADP for oxidative phosphorylation (OxPhos). Adenosine Diphosphate 128-131 hexokinase 1 Homo sapiens 0-10 29119535-3 2018 Hexokinase (HK) is a key enzyme involved in glucose metabolism that modulates the level of brain mitochondrial ROS by recycling ADP for oxidative phosphorylation (OxPhos). Adenosine Diphosphate 128-131 hexokinase 1 Homo sapiens 12-14 29090276-1 2017 BACKGROUND: Hexokinase and glucokinase enzymes are ubiquitously expressed and use ATP and ADP as substrates in mammalian systems and a variety of polyphosphate substrates and/or ATP in some eukaryotic and microbial systems. Adenosine Diphosphate 90-93 hexokinase 1 Homo sapiens 12-22