PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2479582-2 1989 Dexamethasone treatment suppresses pAFP-CAT expression in McA-RH7777 cells but increases its expression in McA-RH8994 cells, which mimics the dexamethasone responses of the endogenous AFP gene in both cell lines. Dexamethasone 0-13 alpha-fetoprotein Rattus norvegicus 36-39 1379951-2 1992 Thus dexamethasone increases the amount of alpha-fetoprotein mRNA in Morris rat hepatoma derived cell line McA-RH 8994 cells, but decreases it in McA-RH 7777 cells. Dexamethasone 5-18 alpha-fetoprotein Rattus norvegicus 43-60 1379951-4 1992 In contrast to the effect of dexamethasone on the alpha-fetoprotein gene expression, which requires ongoing protein synthesis, cycloheximide, a protein synthesis inhibitor, enhanced the effect of retinoic acid. Dexamethasone 29-42 alpha-fetoprotein Rattus norvegicus 50-67 2441991-0 1987 Modulation of alpha-fetoprotein, albumin and transferrin gene expression by cellular interactions and dexamethasone in cocultures of fetal rat hepatocytes. Dexamethasone 102-115 alpha-fetoprotein Rattus norvegicus 14-31 2481456-9 1989 Treatment of the hepatoma and liver cell lines with dexamethasone, which influences AFP gene expression, did not result in any changes in methylation of these sites in the 5" flanking region. Dexamethasone 52-65 alpha-fetoprotein Rattus norvegicus 84-87 2454390-3 1988 Stable transfection assays were more sensitive and accurate than transient expression assays in a Morris 7777 rat hepatoma recipient (Hepa7.6), selected for its strong AFP repression by dexamethasone. Dexamethasone 186-199 alpha-fetoprotein Rattus norvegicus 168-171 2473929-12 1989 Results from tritium labeled dexamethasone competition assays suggest that ZMS may act on AFP gene expression through glucocorticoid receptor mediated action. Dexamethasone 29-42 alpha-fetoprotein Rattus norvegicus 90-93 2441991-4 1987 Addition of dexamethasone to coculture inhibited alpha-fetoprotein secretion and maintained the synthesis rate of albumin and transferrin together with an additional inhibition of DNA synthesis. Dexamethasone 12-25 alpha-fetoprotein Rattus norvegicus 49-66 2441991-6 1987 We observed that the levels of alpha-fetoprotein, albumin and transferrin secretion in cocultures maintained in the presence or absence of dexamethasone were well correlated with the relative amounts of their corresponding mRNAs. Dexamethasone 139-152 alpha-fetoprotein Rattus norvegicus 31-48 2420790-0 1986 Mechanism of the dexamethasone effect on alpha-fetoprotein gene expression in McA-RH8994 rat hepatoma cells. Dexamethasone 17-30 alpha-fetoprotein Rattus norvegicus 41-58 2428823-7 1986 Irrespective of the combinations of growth-promoting factors used, dexamethasone inhibited alpha 1-fetoprotein production and maintained albumin production and tyrosine aminotransferase inducibility. Dexamethasone 67-80 alpha-fetoprotein Rattus norvegicus 91-110 2436613-4 1987 However, methylation patterns of cell lines which were treated with dexamethasone were not different from those of control cells, indicating that glucocorticoid action on AFP gene expression does not alter DNA methylation in this region of the gene. Dexamethasone 68-81 alpha-fetoprotein Rattus norvegicus 171-174 2420790-1 1986 We have investigated the mechanism by which dexamethasone (DEX) increases alpha-fetoprotein (AFP) secretion by the McA-RH8994 rat hepatoma cell line. Dexamethasone 44-57 alpha-fetoprotein Rattus norvegicus 74-91 2420790-1 1986 We have investigated the mechanism by which dexamethasone (DEX) increases alpha-fetoprotein (AFP) secretion by the McA-RH8994 rat hepatoma cell line. Dexamethasone 44-57 alpha-fetoprotein Rattus norvegicus 93-96 2420790-1 1986 We have investigated the mechanism by which dexamethasone (DEX) increases alpha-fetoprotein (AFP) secretion by the McA-RH8994 rat hepatoma cell line. Dexamethasone 59-62 alpha-fetoprotein Rattus norvegicus 74-91 2420790-1 1986 We have investigated the mechanism by which dexamethasone (DEX) increases alpha-fetoprotein (AFP) secretion by the McA-RH8994 rat hepatoma cell line. Dexamethasone 59-62 alpha-fetoprotein Rattus norvegicus 93-96 2420790-2 1986 In contrast to the 7777 cell line, DEX was found to increase the amount of hybridizable cytoplasmic AFP mRNA in McA-RH8994 cells. Dexamethasone 35-38 alpha-fetoprotein Rattus norvegicus 100-103 2420790-5 1986 Cycloheximide blocked the effect of DEX on AFP mRNA levels in both the McA-RH8994 and 7777 cell lines, showing that ongoing protein synthesis is required for glucocorticoids either to enhance or to suppress AFP gene expression in these cell lines. Dexamethasone 36-39 alpha-fetoprotein Rattus norvegicus 43-46 2420790-5 1986 Cycloheximide blocked the effect of DEX on AFP mRNA levels in both the McA-RH8994 and 7777 cell lines, showing that ongoing protein synthesis is required for glucocorticoids either to enhance or to suppress AFP gene expression in these cell lines. Dexamethasone 36-39 alpha-fetoprotein Rattus norvegicus 207-210 2582363-5 1985 Dexamethasone treatment of developing rat liver results in the loss of transcribing polymerases from all regions of the AFP gene. Dexamethasone 0-13 alpha-fetoprotein Rattus norvegicus 120-123 2431944-0 1986 Activation of alpha-fetoprotein synthesis in rat hepatoma cells with reduced sensitivity to dexamethasone. Dexamethasone 92-105 alpha-fetoprotein Rattus norvegicus 14-31 2431944-2 1986 AFP production was activated in "differentiated" variants of Faza 967 cells with reduced glucocorticoid sensitivity upon culture for several months in the presence of high concentrations of dexamethasone. Dexamethasone 190-203 alpha-fetoprotein Rattus norvegicus 0-3 2415528-3 1985 The addition of 5 X 10(-7) M dexamethasone to the medium stimulated the excretion of AFP without increasing total AFP synthesis, whereas 8.7 X 10(-8) M insulin inhibited the excretion of AFP without a significant inhibition of intracellular AFP synthesis. Dexamethasone 29-42 alpha-fetoprotein Rattus norvegicus 85-88 2415528-3 1985 The addition of 5 X 10(-7) M dexamethasone to the medium stimulated the excretion of AFP without increasing total AFP synthesis, whereas 8.7 X 10(-8) M insulin inhibited the excretion of AFP without a significant inhibition of intracellular AFP synthesis. Dexamethasone 29-42 alpha-fetoprotein Rattus norvegicus 114-117 2415528-3 1985 The addition of 5 X 10(-7) M dexamethasone to the medium stimulated the excretion of AFP without increasing total AFP synthesis, whereas 8.7 X 10(-8) M insulin inhibited the excretion of AFP without a significant inhibition of intracellular AFP synthesis. Dexamethasone 29-42 alpha-fetoprotein Rattus norvegicus 114-117 2415528-3 1985 The addition of 5 X 10(-7) M dexamethasone to the medium stimulated the excretion of AFP without increasing total AFP synthesis, whereas 8.7 X 10(-8) M insulin inhibited the excretion of AFP without a significant inhibition of intracellular AFP synthesis. Dexamethasone 29-42 alpha-fetoprotein Rattus norvegicus 114-117 2415528-8 1985 Dexamethasone, 5 X 10(-7) M, caused a marked enhancement in the rate of AFP secretion, with 50% released after 75 min. Dexamethasone 0-13 alpha-fetoprotein Rattus norvegicus 72-75 2408747-5 1985 Sodium butyrate was found to reduce AFP mRNA levels in both dexamethasone-treated 7777 and McA-RH8994 cells; dexamethasone decreases AFP mRNA levels in the former cell line and increases AFP mRNA levels in the latter. Dexamethasone 60-73 alpha-fetoprotein Rattus norvegicus 36-39 2408747-5 1985 Sodium butyrate was found to reduce AFP mRNA levels in both dexamethasone-treated 7777 and McA-RH8994 cells; dexamethasone decreases AFP mRNA levels in the former cell line and increases AFP mRNA levels in the latter. Dexamethasone 109-122 alpha-fetoprotein Rattus norvegicus 133-136 2408747-5 1985 Sodium butyrate was found to reduce AFP mRNA levels in both dexamethasone-treated 7777 and McA-RH8994 cells; dexamethasone decreases AFP mRNA levels in the former cell line and increases AFP mRNA levels in the latter. Dexamethasone 109-122 alpha-fetoprotein Rattus norvegicus 133-136 2409527-0 1985 Dexamethasone inhibits alpha-fetoprotein gene transcription in neonatal rat liver and isolated nuclei. Dexamethasone 0-13 alpha-fetoprotein Rattus norvegicus 23-40 2409527-1 1985 The effect of dexamethasone on rat alpha-fetoprotein (AFP) expression has been further examined. Dexamethasone 14-27 alpha-fetoprotein Rattus norvegicus 35-52 2409527-1 1985 The effect of dexamethasone on rat alpha-fetoprotein (AFP) expression has been further examined. Dexamethasone 14-27 alpha-fetoprotein Rattus norvegicus 54-57 2409527-2 1985 Quantitation of serum AFP levels from newborns treated with dexamethasone showed a dose-response relationship between the quantity of dexamethasone administered and the reduction in AFP serum level. Dexamethasone 60-73 alpha-fetoprotein Rattus norvegicus 22-25 2409527-2 1985 Quantitation of serum AFP levels from newborns treated with dexamethasone showed a dose-response relationship between the quantity of dexamethasone administered and the reduction in AFP serum level. Dexamethasone 60-73 alpha-fetoprotein Rattus norvegicus 182-185 2409527-2 1985 Quantitation of serum AFP levels from newborns treated with dexamethasone showed a dose-response relationship between the quantity of dexamethasone administered and the reduction in AFP serum level. Dexamethasone 134-147 alpha-fetoprotein Rattus norvegicus 22-25 2409527-2 1985 Quantitation of serum AFP levels from newborns treated with dexamethasone showed a dose-response relationship between the quantity of dexamethasone administered and the reduction in AFP serum level. Dexamethasone 134-147 alpha-fetoprotein Rattus norvegicus 182-185 2409527-3 1985 RNA blots, utilizing cloned AFP cDNA as probe, showed a marked reduction in AFP mRNA in dexamethasone treated livers. Dexamethasone 88-101 alpha-fetoprotein Rattus norvegicus 28-31 2409527-3 1985 RNA blots, utilizing cloned AFP cDNA as probe, showed a marked reduction in AFP mRNA in dexamethasone treated livers. Dexamethasone 88-101 alpha-fetoprotein Rattus norvegicus 76-79 2409527-4 1985 The extent of AFP mRNA depletion was correlated with dexamethasone dosage. Dexamethasone 53-66 alpha-fetoprotein Rattus norvegicus 14-17 2409527-5 1985 The effect of dexamethasone on AFP mRNA concentration was relatively rapid; a substantial reduction occurred 12 hours after a single injection. Dexamethasone 14-27 alpha-fetoprotein Rattus norvegicus 31-34 2409527-7 1985 One putative AFP nuclear RNA precursor was identified which rapidly disappeared following dexamethasone treatment. Dexamethasone 90-103 alpha-fetoprotein Rattus norvegicus 13-16 6202403-2 1984 Treatment of rats with dexamethasone strongly reduced (by 83 to 98%) the serum levels of AFP in rats bearing Morris hepatomas 7777, 8994, 7288c , and 9618A2 . Dexamethasone 23-36 alpha-fetoprotein Rattus norvegicus 89-92 6202403-3 1984 Reduced AFP levels were due in part to a large reduction in tumor load in dexamethasone-treated rats. Dexamethasone 74-87 alpha-fetoprotein Rattus norvegicus 8-11 6202403-7 1984 RNA dot hybridization also indicated that dexamethasone reduced the amount of AFP mRNA in hepatoma 7777 while increasing albumin mRNA. Dexamethasone 42-55 alpha-fetoprotein Rattus norvegicus 78-81 6202403-8 1984 Two-dimensional gel electrophoresis of tumor cytosol proteins showed that dexamethasone reduced synthesis of all AFP variants which could be detected by this technique. Dexamethasone 74-87 alpha-fetoprotein Rattus norvegicus 113-116 6203525-4 1984 Treatment of neonatal rats with dexamethasone caused a decrease in liver AFP mRNA levels, but a similar decrease was not apparent in kidney. Dexamethasone 32-45 alpha-fetoprotein Rattus norvegicus 73-76 6178743-2 1982 Radioimmunoassay of media from cell cultures grown in the presence of glucocorticoid (dexamethasone or cortisol) showed a reduction in AFP, an increase in albumin, and no significant change in transferrin accumulation, as compared to controls. Dexamethasone 86-99 alpha-fetoprotein Rattus norvegicus 135-138 6178743-3 1982 Labeling experiments with L-[35S]methionine indicated that in both cells and media of dexamethasone-treated cultures there was a 50--80% reduction in polypeptide precipitated by anti-AFP serum, as compared with controls; no change was seen in polypeptide precipitated by anti-transferrin serum. Dexamethasone 86-99 alpha-fetoprotein Rattus norvegicus 183-186 6178743-4 1982 Pulse and pulse-chase experiments demonstrated that dexamethasone inhibited the synthesis of AFP but not its secretion. Dexamethasone 52-65 alpha-fetoprotein Rattus norvegicus 93-96 6178743-5 1982 The half-time for secretion of AFP in the presence and absence of dexamethasone was 43 min. Dexamethasone 66-79 alpha-fetoprotein Rattus norvegicus 31-34 6194818-0 1983 Rapid suppression of alpha 1-fetoprotein gene transcription by dexamethasone in developing rat liver. Dexamethasone 63-76 alpha-fetoprotein Rattus norvegicus 21-40 6194818-8 1983 The nuclear concentration of AFP mRNA, monitored by dot-blot hybridization, decreases to undetectable levels within 48 h, whereas that of albumin mRNA increases slightly, which indicates the selectivity of DEX action. Dexamethasone 206-209 alpha-fetoprotein Rattus norvegicus 29-32 6194818-9 1983 These results show that DEX suppresses AFP gene expression at the transcriptional level and suggest a direct negative action of DEX-receptor complexes on the AFP chromatin transcription unit. Dexamethasone 24-27 alpha-fetoprotein Rattus norvegicus 39-42 6194818-9 1983 These results show that DEX suppresses AFP gene expression at the transcriptional level and suggest a direct negative action of DEX-receptor complexes on the AFP chromatin transcription unit. Dexamethasone 24-27 alpha-fetoprotein Rattus norvegicus 158-161 6189444-3 1982 The molecular basis of this hormonal action was investigated by examining the steady-state levels of AFP mRNA and albumin mRNA sequences in polysomal and total RNA preparations isolated from dexamethasone-treated and control animals. Dexamethasone 191-204 alpha-fetoprotein Rattus norvegicus 101-104 6189444-4 1982 Following dexamethasone treatment the number of polysomal and total mRNA sequences hybridizable to specific (32P) cDNA probes was drastically decreased for AFP while it was unchanged for albumin. Dexamethasone 10-23 alpha-fetoprotein Rattus norvegicus 156-159 6174948-2 1981 Daily intraperitoneal injection of 2 micrograms/g body weight of dexamethasone into newborn rats greatly reduced the concentration of AFP in the serum and liver cytosol. Dexamethasone 65-78 alpha-fetoprotein Rattus norvegicus 134-137 6174948-6 1981 The size of AFP polysomes isolated from the livers of dexamethasone-treated rats were as large as those from normal rats. Dexamethasone 54-67 alpha-fetoprotein Rattus norvegicus 12-15 6174948-8 1981 By hybridization assays, it was found that dexamethasone treated livers contained decreased amounts of AFP mRNA sequences in liver cytoplasmic and nuclear RNAs. Dexamethasone 43-56 alpha-fetoprotein Rattus norvegicus 103-106 92316-0 1979 The antagonistic effect of dexamethasone and insulin on alpha-fetoprotein secretion by cultured H4-II-E-C3 cells derived from the Reuber H-35 hepatoma. Dexamethasone 27-40 alpha-fetoprotein Rattus norvegicus 56-73 9628324-0 1998 Rat yolk sac explants as a system for studying the regulation of endodermal genes: down-regulation of the alpha-fetoprotein gene by dexamethasone and phorbol ester. Dexamethasone 132-145 alpha-fetoprotein Rattus norvegicus 106-123 9628324-9 1998 Dexamethasone and phorbol ester (TPA) specifically reduced the AFP mRNA level without affecting that of DBP. Dexamethasone 0-13 alpha-fetoprotein Rattus norvegicus 63-66