PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10956052-0 2000 Cholesterol efflux to high-density lipoproteins and apolipoprotein A-I phosphatidylcholine complexes is inhibited by ethanol: role of apolipoprotein structure and cooperative interaction of phosphatidylcholine and cholesterol. Ethanol 117-124 apolipoprotein A1 Homo sapiens 52-70 10956052-5 2000 In this paper, we show that ethanol significantly inhibited cholesterol efflux from fibroblasts to HDL and to apolipoprotein A-I (apoA-I) complexed with phosphatidylcholine (PC). Ethanol 28-35 apolipoprotein A1 Homo sapiens 110-128 10956052-5 2000 In this paper, we show that ethanol significantly inhibited cholesterol efflux from fibroblasts to HDL and to apolipoprotein A-I (apoA-I) complexed with phosphatidylcholine (PC). Ethanol 28-35 apolipoprotein A1 Homo sapiens 130-136 10956052-6 2000 Ethanol significantly inhibited binding of PC to apoA-I, inhibited incorporation of cholesterol only when apoA-I contained PC, and did not alter incorporation of cholesterol into HDL. Ethanol 0-7 apolipoprotein A1 Homo sapiens 49-55 10956052-6 2000 Ethanol significantly inhibited binding of PC to apoA-I, inhibited incorporation of cholesterol only when apoA-I contained PC, and did not alter incorporation of cholesterol into HDL. Ethanol 0-7 apolipoprotein A1 Homo sapiens 106-112 10956052-7 2000 ApoA-I structure was altered by ethanol as monitored by steady-state fluorescence polarization of tryptophan residues. Ethanol 32-39 apolipoprotein A1 Homo sapiens 0-6 10956052-9 2000 In addition, effects of ethanol on apoA-I suggest that pre-beta-HDL or lipid-free apoA-I may be more perturbed by ethanol than mature HDL, and such effects may be pathophysiological with respect to the process of reverse cholesterol transport in heavy drinkers and alcoholics. Ethanol 24-31 apolipoprotein A1 Homo sapiens 35-41 10956052-9 2000 In addition, effects of ethanol on apoA-I suggest that pre-beta-HDL or lipid-free apoA-I may be more perturbed by ethanol than mature HDL, and such effects may be pathophysiological with respect to the process of reverse cholesterol transport in heavy drinkers and alcoholics. Ethanol 114-121 apolipoprotein A1 Homo sapiens 35-41 10956052-9 2000 In addition, effects of ethanol on apoA-I suggest that pre-beta-HDL or lipid-free apoA-I may be more perturbed by ethanol than mature HDL, and such effects may be pathophysiological with respect to the process of reverse cholesterol transport in heavy drinkers and alcoholics. Ethanol 114-121 apolipoprotein A1 Homo sapiens 82-88 10591709-8 1999 An experimental dose of 30 g of ethanol a day increased concentrations of high density lipoprotein cholesterol by 3.99 mg/dl (95% confidence interval 3.25 to 4.73), apolipoprotein A I by 8.82 mg/dl (7.79 to 9.86), and triglyceride by 5.69 mg/dl (2.49 to 8.89). Ethanol 32-39 apolipoprotein A1 Homo sapiens 165-183 1335218-6 1992 Exposure of HepG2 and Hep3B cells to ethanol resulted in an increase of accumulation of apoA-I (15%-45% over control) in a dose-dependent manner (from 5 to 50 mM) of ethanol over a 24-hr period. Ethanol 37-44 apolipoprotein A1 Homo sapiens 88-94 8732781-0 1996 Effect of ethanol on the synthesis and secretion of apoA-I- and apoB-containing lipoproteins in HepG2 cells. Ethanol 10-17 apolipoprotein A1 Homo sapiens 52-58 8732781-11 1996 In contrast to the short-term effect of ethanol, long-term incubation with ethanol resulted in a dose-dependent increase in the secretion of newly synthesized apoA-I without significantly affecting that of apoB-100. Ethanol 75-82 apolipoprotein A1 Homo sapiens 159-165 8732781-12 1996 The increase in apoA-I secretion was evident at 0.05% ethanol and reached a maximum of 77% at 0.5% ethanol. Ethanol 54-61 apolipoprotein A1 Homo sapiens 16-22 8732781-12 1996 The increase in apoA-I secretion was evident at 0.05% ethanol and reached a maximum of 77% at 0.5% ethanol. Ethanol 99-106 apolipoprotein A1 Homo sapiens 16-22 8732781-13 1996 These observations indicate that in HepG2 cells the effect of ethanol on the production of apoA-I- and apoB- containing lipoproteins is both time- and dose-dependent and is different in these two apolipoproteins. Ethanol 62-69 apolipoprotein A1 Homo sapiens 91-97 8912458-2 1996 Apolipoprotein A-I (apoA-I was isolated from precipitates obtained by cold ethanol fractionation of human plasma. Ethanol 75-82 apolipoprotein A1 Homo sapiens 0-18 8912458-2 1996 Apolipoprotein A-I (apoA-I was isolated from precipitates obtained by cold ethanol fractionation of human plasma. Ethanol 75-82 apolipoprotein A1 Homo sapiens 20-26 8332287-0 1993 Ethanol stimulates apo A-1 secretion in human hepatocytes: a possible mechanism underlying the cardioprotective effect of ethanol. Ethanol 0-7 apolipoprotein A1 Homo sapiens 19-26 8332287-0 1993 Ethanol stimulates apo A-1 secretion in human hepatocytes: a possible mechanism underlying the cardioprotective effect of ethanol. Ethanol 122-129 apolipoprotein A1 Homo sapiens 19-26 8332287-1 1993 Ethanol stimulates synthesis of the high-density lipoprotein (HDL) protein apo A-1 in cultured human hepatocytes. Ethanol 0-7 apolipoprotein A1 Homo sapiens 75-82 8332287-3 1993 Enhanced activity of a reverse cholesterol transport pathway mediated by increased hepatic apo A-1 synthesis may explain why epidemiological studies show a relationship between ethanol consumption and lower incidence of coronary heart disease. Ethanol 177-184 apolipoprotein A1 Homo sapiens 91-98 1335218-6 1992 Exposure of HepG2 and Hep3B cells to ethanol resulted in an increase of accumulation of apoA-I (15%-45% over control) in a dose-dependent manner (from 5 to 50 mM) of ethanol over a 24-hr period. Ethanol 166-173 apolipoprotein A1 Homo sapiens 88-94 1834916-7 1991 Ethanol intake caused an increase in the serum levels of both Lp A-I and Lp A-I:A-II, the former explaining one third of the total increase of apo A-I. Ethanol 0-7 apolipoprotein A1 Homo sapiens 143-150 1640859-0 1992 Ethanol stimulates apolipoprotein A-I secretion by human hepatocytes: implications for a mechanism for atherosclerosis protection. Ethanol 0-7 apolipoprotein A1 Homo sapiens 19-37 1640859-4 1992 Using highly specific antibodies, we have found that ethanol increases apo A-I secretion and the incorporation of radiolabeled leucine into apo A-I by human hepatocytes (Hep-G2 cells). Ethanol 53-60 apolipoprotein A1 Homo sapiens 71-76 1640859-4 1992 Using highly specific antibodies, we have found that ethanol increases apo A-I secretion and the incorporation of radiolabeled leucine into apo A-I by human hepatocytes (Hep-G2 cells). Ethanol 53-60 apolipoprotein A1 Homo sapiens 140-145 1640859-5 1992 In addition, we have found that apo A-I molecules induced by ethanol have the ability to efflux cholesterol from human fibroblasts in vitro, and that apo A-I mass directly correlates with cholesterol efflux levels. Ethanol 61-68 apolipoprotein A1 Homo sapiens 32-37 1640859-5 1992 In addition, we have found that apo A-I molecules induced by ethanol have the ability to efflux cholesterol from human fibroblasts in vitro, and that apo A-I mass directly correlates with cholesterol efflux levels. Ethanol 61-68 apolipoprotein A1 Homo sapiens 32-39 1640859-6 1992 At 10, 20, and 100 mmol/L, ethanol stimulated apo A-I secretion by 130%, 136%, and 162% of control, respectively (control, 3.71 micrograms apo A-I/micrograms DNA), also stimulating the incorporation of 3H-leucine into newly synthesized apo A-I by 115%, 131%, and 159% of control (control, 111 cpm/micrograms DNA/h). Ethanol 27-34 apolipoprotein A1 Homo sapiens 46-51 1640859-6 1992 At 10, 20, and 100 mmol/L, ethanol stimulated apo A-I secretion by 130%, 136%, and 162% of control, respectively (control, 3.71 micrograms apo A-I/micrograms DNA), also stimulating the incorporation of 3H-leucine into newly synthesized apo A-I by 115%, 131%, and 159% of control (control, 111 cpm/micrograms DNA/h). Ethanol 27-34 apolipoprotein A1 Homo sapiens 139-144 1640859-6 1992 At 10, 20, and 100 mmol/L, ethanol stimulated apo A-I secretion by 130%, 136%, and 162% of control, respectively (control, 3.71 micrograms apo A-I/micrograms DNA), also stimulating the incorporation of 3H-leucine into newly synthesized apo A-I by 115%, 131%, and 159% of control (control, 111 cpm/micrograms DNA/h). Ethanol 27-34 apolipoprotein A1 Homo sapiens 139-144 1640859-7 1992 The ethanol-induced apo A-I from Hep-G2 cells (incubated with 0, 10, 20, and 100 mmol/L ethanol) effluxed 2%, 14%, 16%, and 32% label (per h/mL incubation medium), respectively. Ethanol 4-11 apolipoprotein A1 Homo sapiens 20-25 1640859-7 1992 The ethanol-induced apo A-I from Hep-G2 cells (incubated with 0, 10, 20, and 100 mmol/L ethanol) effluxed 2%, 14%, 16%, and 32% label (per h/mL incubation medium), respectively. Ethanol 88-95 apolipoprotein A1 Homo sapiens 20-25 1640859-9 1992 This data indicates that the cardioprotective role of moderate ethanol intake in humans is mediated by its stimulatory action on hepatic apo A-I secretion, thus defining the physiological basis for increased plasma apo A-I levels in vivo. Ethanol 63-70 apolipoprotein A1 Homo sapiens 137-142 1640859-9 1992 This data indicates that the cardioprotective role of moderate ethanol intake in humans is mediated by its stimulatory action on hepatic apo A-I secretion, thus defining the physiological basis for increased plasma apo A-I levels in vivo. Ethanol 63-70 apolipoprotein A1 Homo sapiens 215-220 2502043-3 1989 Through the use of urea, chloroform, and ethanol in appropriate concentrations, apolipoproteins A-I and A-II were isolated by a simple extraction technique avoiding time-consuming ultracentrifugation. Ethanol 41-48 apolipoprotein A1 Homo sapiens 80-108 2502043-1 1989 Apolipoproteins A-I and A-II were isolated from precipitates obtained by cold ethanol fractionation of human plasma. Ethanol 78-85 apolipoprotein A1 Homo sapiens 0-28 2502043-6 1989 Apolipoprotein A-I obtained by the cold ethanol method activated lecithin-cholesterol acyltransferase to the same extent as apolipoprotein A-I prepared by the classical flotation method. Ethanol 40-47 apolipoprotein A1 Homo sapiens 0-18 2650924-4 1989 Hep G2 cells when grown in ethanol-containing medium for 14-28 days manifested increased HDL-binding capacity; apolipoprotein-A1 secretion was increased by ethanol but apolipoprotein B secretion was not affected. Ethanol 27-34 apolipoprotein A1 Homo sapiens 111-128 2650924-4 1989 Hep G2 cells when grown in ethanol-containing medium for 14-28 days manifested increased HDL-binding capacity; apolipoprotein-A1 secretion was increased by ethanol but apolipoprotein B secretion was not affected. Ethanol 156-163 apolipoprotein A1 Homo sapiens 111-128 6810694-7 1982 Apo A-I level was unrelated to age, but increased with ethanol consumption and decreased with adiposity. Ethanol 55-62 apolipoprotein A1 Homo sapiens 0-7 3365983-4 1988 At baseline, ethanol consumption was found to be positively associated with triglycerides, HDL-C, and Apo-A1 and negatively associated with LDL-C/LDL-B. Ethanol 13-20 apolipoprotein A1 Homo sapiens 102-108 3933859-3 1985 Mean synthesis of Apo A-I and B were increased, respectively, from 12.6 and 13.7 mg/kg per day in the absence of ethanol to 18.8 and 17.1 mg/kg/day after 2 wk of ethanol intake. Ethanol 113-120 apolipoprotein A1 Homo sapiens 18-31 3933859-3 1985 Mean synthesis of Apo A-I and B were increased, respectively, from 12.6 and 13.7 mg/kg per day in the absence of ethanol to 18.8 and 17.1 mg/kg/day after 2 wk of ethanol intake. Ethanol 162-169 apolipoprotein A1 Homo sapiens 18-31 3933859-4 1985 Fractional catabolic rates for Apo A-I and B increased respectively from 0.204 and 0.340 in the period without ethanol to 0.266 and 0.372 after ethanol. Ethanol 111-118 apolipoprotein A1 Homo sapiens 31-44 3933859-4 1985 Fractional catabolic rates for Apo A-I and B increased respectively from 0.204 and 0.340 in the period without ethanol to 0.266 and 0.372 after ethanol. Ethanol 144-151 apolipoprotein A1 Homo sapiens 31-44 6782187-4 1980 Delipidation with ether/ethanol produced a decrease of apolipoprotein A-I values in both methods as compared to treatment with tetramethyl urea. Ethanol 24-31 apolipoprotein A1 Homo sapiens 55-73 32563265-0 2020 Ethanol-mediated upregulation of APOA1 gene expression in HepG2 cells is independent of de novo lipid biosynthesis. Ethanol 0-7 apolipoprotein A1 Homo sapiens 33-38 32563265-6 2020 RESULTS: Ethanol stimulated ApoA1 protein (both cytoplasmic and secreted) and APOA1 RNA levels in HepG2 cells in a dose sensitive way, with ~ 50% upregulation at 100 mM ethanol in the medium. Ethanol 9-16 apolipoprotein A1 Homo sapiens 28-33 32563265-6 2020 RESULTS: Ethanol stimulated ApoA1 protein (both cytoplasmic and secreted) and APOA1 RNA levels in HepG2 cells in a dose sensitive way, with ~ 50% upregulation at 100 mM ethanol in the medium. Ethanol 9-16 apolipoprotein A1 Homo sapiens 78-83 32563265-6 2020 RESULTS: Ethanol stimulated ApoA1 protein (both cytoplasmic and secreted) and APOA1 RNA levels in HepG2 cells in a dose sensitive way, with ~ 50% upregulation at 100 mM ethanol in the medium. Ethanol 169-176 apolipoprotein A1 Homo sapiens 28-33 32563265-14 2020 CONCLUSION: This study has verified the previously reported upregulation of APOA1 by exposure of HepG2, but not Caco-2 cells, to ethanol in the culture medium. Ethanol 129-136 apolipoprotein A1 Homo sapiens 76-81 32563265-17 2020 The results reported here suggest that the proximal signaling molecule leading to increased APOA1 gene expression in response to ethanol exposure may be free acetate or acetyl-CoA. Ethanol 129-136 apolipoprotein A1 Homo sapiens 92-97 32563265-18 2020 TAKE HOME: Upregulation of ApoA1 gene expression in hepatoma cells in culture, upon exposure to moderate ethanol concentrations in the medium, occurs at the level of RNA and is not dependent on new cholesterol or fatty acid synthesis. Ethanol 105-112 apolipoprotein A1 Homo sapiens 27-32 24912423-11 2014 As supplement in PLCs, nHDL3 or apoA-I from Fraction IV of plasma ethanol fractionation have the potential to improve PLC quality to prolong storage. Ethanol 66-73 apolipoprotein A1 Homo sapiens 32-38 32223124-3 2020 Here, we show that a dHDL-relevant complex can also be prepared by gently mixing 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and apoA-I or its mutants in ethanol/H2O solutions containing urea in the order of a few molar, and then incubating the mixture at the gel-liquid crystalline phase transition temperature in test tubes. Ethanol 161-168 apolipoprotein A1 Homo sapiens 136-142 15686978-14 2005 Ethanol extraction may also not be advisable in all other situations known to be associated with a rise in apolipoprotein A-1 turnover. Ethanol 0-7 apolipoprotein A1 Homo sapiens 107-125