PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
8361957-0	1993	Selective extraction of alkaline phosphatase and 5"-nucleotidase from milk fat globule membranes by a single phase n-butanol procedure.	1-Butanol	115-124	5'-nucleotidase	Bos taurus	49-64	
8361957-2	1993	For 5"-nucleotidase, higher n-butanol concentrations lead to loss of activity, while lower concentrations were ineffective in extracting the enzyme.	1-Butanol	28-37	5'-nucleotidase	Bos taurus	4-19	
8361957-3	1993	When extractions were performed at 0 degrees C, similar yields were obtained for alkaline phosphatase extraction with 8% (v/v) n-butanol, but 5"-nucleotidase extraction required 10% (v/v) n-butanol for similar yields.	1-Butanol	188-197	5'-nucleotidase	Bos taurus	142-157	
8361957-4	1993	However, 5"-nucleotidase was less susceptible to denaturation during extraction at 0 degrees C. The Km values and substrate specificities for both alkaline phosphatase and 5"-nucleotidase were unchanged by extraction with 8% (v/v) n-butanol.	1-Butanol	231-240	5'-nucleotidase	Bos taurus	172-187