PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20363235-4 2010 Amitriptyline, nortriptyline and imipramine showed considerably higher affinities for alpha(1A)- (approximately 25- to 80-fold) and alpha(1D)-adrenoceptors (approximately 10- to 25-fold) than for alpha(1B)-adrenoceptors in both contraction and [(3)H]prazosin binding assays with rat native and human receptors, respectively. Prazosin 250-258 calcium voltage-gated channel subunit alpha1 A Homo sapiens 86-94 17302998-7 2007 Whole cell [3H]-prazosin binding assay showed that alpha1A-AR functional receptors were detected both on the cell surface and in the cytoplasm; alpha1B-AR, however, were detected predominantly on the cell surface, while alpha1D-AR were detected mainly in intracellular compartments. Prazosin 16-24 calcium voltage-gated channel subunit alpha1 A Homo sapiens 51-58 18336813-4 2008 In contrast, the affinities of alpha1A-, alpha1B-, and alpha1D-adrenoceptors for prazosin, silodosin, and BMY 7378 did not significantly change under different assay conditions and in different tissues. Prazosin 81-89 calcium voltage-gated channel subunit alpha1 A Homo sapiens 31-38 9556189-2 1998 Subsequent characterization of alpha1-adrenoceptors using radioligand binding and functional studies has led to the identification of three native prazosin high-affinity alpha1-adrenoceptor subtypes designated alpha1A, alpha1B and alpha1D, corresponding to the three alpha1-adrenoceptor subtypes (alpha1a, alpha1b and alpha1d) isolated by molecular cloning techniques. Prazosin 147-155 calcium voltage-gated channel subunit alpha1 A Homo sapiens 210-217 11602687-8 2001 Prazosin, 5-methyl-urapidil, and 2-[2,6-dimethoxyphenoxyethyl]aminomethyl)-1,4-benzodioxane (WB 4101) shifted the potency of norepinephrine concentration dependently giving pA2 values of 9.4, 8.9, and 10.1, respectively, showing the presence of the alpha1A-subtype in these arteries. Prazosin 0-8 calcium voltage-gated channel subunit alpha1 A Homo sapiens 249-256 10598792-3 1999 The specific binding of [3H]prazosin to membranes of U373 MG cells (Bmax 32+/-3 fmol mg(-1) protein, Kd 0.27+/-0.03 nM) was inhibited in a monophasic manner by alpha1-antagonists that have different affinities for alpha1A-, alpha1B- and alpha1D-adrenoceptors. Prazosin 28-36 calcium voltage-gated channel subunit alpha1 A Homo sapiens 214-221 9822553-2 1998 Prazosin-related quinazolines 4-20 were synthesized, and their biological profiles at alpha1-adrenoreceptor subtypes were assessed by functional experiments in isolated rat vas deferens (alpha1A), spleen (alpha1B), and aorta (alpha1D) and by binding assays in CHO cells expressing human cloned alpha1-adrenoreceptor subtypes. Prazosin 0-8 calcium voltage-gated channel subunit alpha1 A Homo sapiens 187-194 15626751-5 2005 In cells expressing a human alpha(1a)-AR-enhanced green fluorescent protein (EGFP) 2 fusion protein, BODIPY-R-558/568-prazosin (RQAPB) colocalized with the fusion, indicating that the ligand gained access to all compartments containing the receptor, and, conversely, that the receptor has affinity for the ligand at all of these sites. Prazosin 118-126 calcium voltage-gated channel subunit alpha1 A Homo sapiens 28-36 11263248-9 2000 Preincubation with prazosin completely abolished NE-induced CCDPK activation in HEK293/alpha 1A- and alpha 1B-AR cells. Prazosin 19-27 calcium voltage-gated channel subunit alpha1 A Homo sapiens 87-95 10024333-6 1999 The pharmacological profile of [3H]prazosin binding to human peripheral blood lymphocytes was consistent with the labeling of alpha1-adrenergic receptors. Prazosin 31-43 calcium voltage-gated channel subunit alpha1 A Homo sapiens 126-153 10024333-7 1999 Antibodies against alpha1A-, alpha1B-, and alpha1D-receptor subtypes decreased [3H]prazosin binding to a different extent. Prazosin 83-91 calcium voltage-gated channel subunit alpha1 A Homo sapiens 19-26 10393465-3 1999 Subsequent characterization of the alpha1-adrenoceptor using radioligand binding and functional studies has led to the identification of three native prazosin-high-affinity alpha1-adrenoceptor subtypes designated alpha1A, alpha1B, and alpha1D, corresponding to the three alpha1-adrenoceptor subtypes (alpha1a, alpha1b, and alpha1d) characterized by molecular cloning techniques. Prazosin 151-159 calcium voltage-gated channel subunit alpha1 A Homo sapiens 214-221 10393465-3 1999 Subsequent characterization of the alpha1-adrenoceptor using radioligand binding and functional studies has led to the identification of three native prazosin-high-affinity alpha1-adrenoceptor subtypes designated alpha1A, alpha1B, and alpha1D, corresponding to the three alpha1-adrenoceptor subtypes (alpha1a, alpha1b, and alpha1d) characterized by molecular cloning techniques. Prazosin 151-159 calcium voltage-gated channel subunit alpha1 A Homo sapiens 302-309 10438241-4 1999 Subsequent characterization of the alpha1-adrenoceptor using radioligand binding and functional studies has let to the identification of the three native prazosin-high-affinity alpha1-adrenoceptor subtypes designated alpha1A, alpha1B, and alpha1D, corresponding to the three alpha1-adrenoceptor subtypes (alpha1a, alpha1b, and alpha1d) characterized by molecular cloning techniques. Prazosin 154-162 calcium voltage-gated channel subunit alpha1 A Homo sapiens 217-224 10438241-4 1999 Subsequent characterization of the alpha1-adrenoceptor using radioligand binding and functional studies has let to the identification of the three native prazosin-high-affinity alpha1-adrenoceptor subtypes designated alpha1A, alpha1B, and alpha1D, corresponding to the three alpha1-adrenoceptor subtypes (alpha1a, alpha1b, and alpha1d) characterized by molecular cloning techniques. Prazosin 154-162 calcium voltage-gated channel subunit alpha1 A Homo sapiens 305-312 9556189-2 1998 Subsequent characterization of alpha1-adrenoceptors using radioligand binding and functional studies has led to the identification of three native prazosin high-affinity alpha1-adrenoceptor subtypes designated alpha1A, alpha1B and alpha1D, corresponding to the three alpha1-adrenoceptor subtypes (alpha1a, alpha1b and alpha1d) isolated by molecular cloning techniques. Prazosin 147-155 calcium voltage-gated channel subunit alpha1 A Homo sapiens 297-304 1686864-0 1991 The alkylating prazosin analog SZL 49 inactivates both alpha 1A- and alpha 1B-adrenoceptors. Prazosin 15-23 calcium voltage-gated channel subunit alpha1 A Homo sapiens 55-63 7781714-9 1995 The pharmacological profile of the [3H]prazosin binding sites of human liver membranes suggests that alpha 1A-adrenoceptors predominate (75%-85% of the alpha 1-adrenoceptors) in this tissue. Prazosin 35-47 calcium voltage-gated channel subunit alpha1 A Homo sapiens 101-109 7913370-14 1994 The affinities of prazosin, WB 4101, 5-methyl-urapidil, phentolamine and benoxathian at cloned alpha 1A/D-receptors were consistent with alpha 1A affinities determined with chlorethylclonidine-treated rat cortical membranes. Prazosin 18-26 calcium voltage-gated channel subunit alpha1 A Homo sapiens 95-103 7691623-2 1993 Saturation experiments showed that [3H]prazosin labelled a single population of binding sites in the spleen (alpha 1B) and hippocampus (alpha 1A and alpha 1B) (dissociation constants (KD): 0.26 nM and 0.14 nM respectively). Prazosin 35-47 calcium voltage-gated channel subunit alpha1 A Homo sapiens 136-144 9249248-5 1997 In the present study, binding affinities were estimated by displacement of [3H]-prazosin in membrane homogenates of Chinese hamster ovary (CHO-K1) cells stably expressing the human alpha1A-, alpha1B- and alpha1D-adrenoceptors and were compared with affinity estimates obtained functionally in identical cells by measuring inhibition of noradrenaline (NA)-stimulated accumulation of [3H]-inositol phosphates. Prazosin 80-88 calcium voltage-gated channel subunit alpha1 A Homo sapiens 181-188 8896043-2 1996 Radioligand binding experiments with [3H]prazosin showed that the maximum number of alpha 1A-adrenoceptors in rat myocardium was about ten times higher than in rabbit myocardium. Prazosin 37-49 calcium voltage-gated channel subunit alpha1 A Homo sapiens 84-92 1686864-1 1991 A chemically reactive prazosin analog (SZL 49) has been suggested to be an alpha 1A-selective alkylating agent. Prazosin 22-30 calcium voltage-gated channel subunit alpha1 A Homo sapiens 75-83 1674110-3 1991 The alpha 1A subtype is visualized by [3H]prazosin after irreversible blockade of alpha 1B adrenoceptors with chloroethylclonidine or directly by [3H]5-methyl-urapidil. Prazosin 38-50 calcium voltage-gated channel subunit alpha1 A Homo sapiens 4-12 21745191-5 2012 In prostate homogenates or membrane preparations, alpha(1A)-adrenoceptors with high affinity for prazosin have been detected as radioligand binding sites. Prazosin 97-105 calcium voltage-gated channel subunit alpha1 A Homo sapiens 50-58 30139713-6 2018 In contrast, relatively low affinity estimates were obtained for both prazosin (pKD = 8.2 +- 0.1) and RS17053 (pKD = 6.9 +- 0.2), antagonists which discriminate between the alpha1A- and alpha1L-adrenoceptors. Prazosin 70-78 calcium voltage-gated channel subunit alpha1 A Homo sapiens 173-180 27903755-4 2017 The 9-aminoacridines increase the rate of [3H]prazosin dissociation from the alpha1A- and alpha1B-adrenergic receptors and noncompetitively inhibit receptor activation by the endogenous agonist norepinephrine. Prazosin 42-54 calcium voltage-gated channel subunit alpha1 A Homo sapiens 77-84 30719698-2 2019 Prazosin has a lower affinity for the lower urinary tract alpha1A -adrenoceptor than alpha1A -adrenoceptors found in other parts of the body. Prazosin 0-8 calcium voltage-gated channel subunit alpha1 A Homo sapiens 58-65 22101021-6 2012 We transiently transfected the alpha(1a)-adrenergic receptor into COS-1 cells and determined its pharmacological characteristics by [(3)H]prazosin binding. Prazosin 138-146 calcium voltage-gated channel subunit alpha1 A Homo sapiens 31-39