PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 17121695-3 2006 HIS-NSE fusion protein expression was obtained in E. coli M15 after IPTG induction followed by purification of the fusion protein by Ni-NTA affinity chromatography. Isopropyl Thiogalactoside 68-72 enolase 2 Homo sapiens 4-7