PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25358004-3 2014 The open reading frame of porcine SAMHD1 was cloned into the prokaryotic expression vector pCold-TF DNA to construct a recombinant plasmid pcold-pSAMHD1 and induce expression of recombinant porcine SAMHD1 protein by IPTG in Escherichia coli Rosetta. Isopropyl Thiogalactoside 216-220 SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 Homo sapiens 34-40