PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31175428-5 2019 The expression level of sfGFP-scFv-His reached at 20 mg/L of bacterial culture when the culture was induced with 0.1 mM IPTG at 18 C for 16 h. And 6 mg scFv-His was obtained from the cleavage of 10 mg pure sfGFP-scFv-His with TEV protease. Isopropyl Thiogalactoside 120-124 immunglobulin heavy chain variable region Homo sapiens 30-34 31175428-5 2019 The expression level of sfGFP-scFv-His reached at 20 mg/L of bacterial culture when the culture was induced with 0.1 mM IPTG at 18 C for 16 h. And 6 mg scFv-His was obtained from the cleavage of 10 mg pure sfGFP-scFv-His with TEV protease. Isopropyl Thiogalactoside 120-124 immunglobulin heavy chain variable region Homo sapiens 153-157 27129873-13 2016 The results showed that the expression level and folding of scFv-Fcs induced at 25 C without isopropyl beta-D-1-thiogalactopyranoside (IPTG) were higher than that induced at 32 C with 1.0 mmol/L IPTG. Isopropyl Thiogalactoside 94-134 immunglobulin heavy chain variable region Homo sapiens 60-64 27129873-13 2016 The results showed that the expression level and folding of scFv-Fcs induced at 25 C without isopropyl beta-D-1-thiogalactopyranoside (IPTG) were higher than that induced at 32 C with 1.0 mmol/L IPTG. Isopropyl Thiogalactoside 136-140 immunglobulin heavy chain variable region Homo sapiens 60-64 27129873-13 2016 The results showed that the expression level and folding of scFv-Fcs induced at 25 C without isopropyl beta-D-1-thiogalactopyranoside (IPTG) were higher than that induced at 32 C with 1.0 mmol/L IPTG. Isopropyl Thiogalactoside 197-201 immunglobulin heavy chain variable region Homo sapiens 60-64 19388277-3 2009 The phage positive clones were transformed into Escherichia coli HB2151 and soluble scFv protein was expressed with the induction of Isopropyl beta-D-1-Thiogalavtopy ranoside (IPTG). Isopropyl Thiogalactoside 176-180 immunglobulin heavy chain variable region Homo sapiens 84-88 26071785-4 2015 In the present study, an anti-carbaryl scFv gene was prepared by cloning VL and VH genes from hybridoma cells secreting monoclonal antibody, then VH and VL were fused together using splicing by overlap extension (SOE) PCR with a flexible polypeptide linker connector (Gly4Ser)3, and then the scFv-pET-26b recombinant plasmid was constructed and transformed into E. coli BL21 for expression using IPTG as an inducer. Isopropyl Thiogalactoside 396-400 immunglobulin heavy chain variable region Homo sapiens 39-43 23994147-2 2013 Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-d-thiogalactopyranoside (IPTG) were obtained. Isopropyl Thiogalactoside 148-152 immunglobulin heavy chain variable region Homo sapiens 82-86 22768862-2 2012 METHODS: Expression of E3 scFv was induced by different concentrations of IPTG under the fixed condition of time period and temperature, and the optimal concentration of IPTG was determined by SDS-PAGE analysis on E3 scFv expression level. Isopropyl Thiogalactoside 74-78 immunglobulin heavy chain variable region Homo sapiens 26-30 22768862-2 2012 METHODS: Expression of E3 scFv was induced by different concentrations of IPTG under the fixed condition of time period and temperature, and the optimal concentration of IPTG was determined by SDS-PAGE analysis on E3 scFv expression level. Isopropyl Thiogalactoside 170-174 immunglobulin heavy chain variable region Homo sapiens 26-30 22768862-2 2012 METHODS: Expression of E3 scFv was induced by different concentrations of IPTG under the fixed condition of time period and temperature, and the optimal concentration of IPTG was determined by SDS-PAGE analysis on E3 scFv expression level. Isopropyl Thiogalactoside 170-174 immunglobulin heavy chain variable region Homo sapiens 217-221 22768862-4 2012 RESULTS: We obtained the highest expression of E3 scFv after 18 h induction with 0.1 mmol/L IPTG under 20 Degrees Celsius. Isopropyl Thiogalactoside 92-96 immunglobulin heavy chain variable region Homo sapiens 50-54 26301925-4 2015 The recombinant scFv/1N8 protein was successfully expressed in recombinant Escherichia coli by IPTG induction. Isopropyl Thiogalactoside 95-99 immunglobulin heavy chain variable region Homo sapiens 16-20 23994147-2 2013 Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-d-thiogalactopyranoside (IPTG) were obtained. Isopropyl Thiogalactoside 108-146 immunglobulin heavy chain variable region Homo sapiens 82-86 23600505-4 2013 The recombinant scFv/6E6 protein was successfully expressed in recombinant Escherichia coli by IPTG induction. Isopropyl Thiogalactoside 95-99 immunglobulin heavy chain variable region Homo sapiens 16-20 21707360-12 2011 The scFv protein from IPTG-induced cells was purified under native conditions by immobilized metal affinity chromatography on a Ni-NTA agarose column. Isopropyl Thiogalactoside 22-26 immunglobulin heavy chain variable region Homo sapiens 4-8 16755932-3 2006 Expression of scFv in transformant was induced with 0.5mmol/L IPTG. Isopropyl Thiogalactoside 62-66 immunglobulin heavy chain variable region Homo sapiens 14-18 16806017-7 2006 RESULTS: After induced with 0.5 mmol/L IPTG overnight at 25 degrees C, the amount of expressed scFv C1 increased greatly and its relative molecular mass was about 28,000, and it existed in culture supernant in soluble form. Isopropyl Thiogalactoside 39-43 immunglobulin heavy chain variable region Homo sapiens 95-99 14679004-4 2003 After isopropyl beta-D-thiogalactoside induction, the 70-kDa His(10)-tagged m22(scFv)-ETA" was directed into the periplasmic space and purified by a combination of metal-ion affinity and molecular size-chromatography. Isopropyl Thiogalactoside 6-38 immunglobulin heavy chain variable region Homo sapiens 80-84 16850734-4 2006 After the induction by IPTG, the scFv protein was identified by SDS-PAGE electrophoresis and purified by Ni-NTA-Chromatography. Isopropyl Thiogalactoside 23-27 immunglobulin heavy chain variable region Homo sapiens 33-37 16850734-7 2006 Id-scFv protein was expressed in positive clone after induced by IPTG. Isopropyl Thiogalactoside 65-69 immunglobulin heavy chain variable region Homo sapiens 3-7 15973987-12 2004 One of the selected phage clones against MCF-7cells was used to infect E. coli TOP10 to produce the soluble scFv antibodies after induction with IPTG. Isopropyl Thiogalactoside 145-149 immunglobulin heavy chain variable region Homo sapiens 108-112 15191715-6 2004 scFv protein was expressed as soluble protein under the condition of a small amount of IPTG induction and culture at lower temperature. Isopropyl Thiogalactoside 87-91 immunglobulin heavy chain variable region Homo sapiens 0-4 12526323-2 1998 The phage DNA was extracted and transformed to E. coli HB2151 that allowed to express soluble ScFv proteins by inducing of IPTG. Isopropyl Thiogalactoside 123-127 immunglobulin heavy chain variable region Homo sapiens 94-98 12479098-5 2002 The latter was cloned into the expression vector PET-28a(+) and induced by IPTG to express a fusion protein scFv and His-tag in E. coli BL-21. Isopropyl Thiogalactoside 75-79 immunglobulin heavy chain variable region Homo sapiens 108-112 9373314-4 1996 RESULTS: Over 100 mg/l of the HNK20-scFv was produced in shake flasks after induction with isopropyl (beta-D-thiogalactopyranoside (IPTG). Isopropyl Thiogalactoside 91-130 immunglobulin heavy chain variable region Homo sapiens 36-40 9015295-3 1997 DNA encoding the scFv was sub-cloned into pDAP2 such that a scFv-alkaline phosphatase fusion protein was produced by transformed bacteria following induction by isopropyl-beta-D-thiogalactopyranoside (IPTG). Isopropyl Thiogalactoside 161-199 immunglobulin heavy chain variable region Homo sapiens 17-21 9015295-3 1997 DNA encoding the scFv was sub-cloned into pDAP2 such that a scFv-alkaline phosphatase fusion protein was produced by transformed bacteria following induction by isopropyl-beta-D-thiogalactopyranoside (IPTG). Isopropyl Thiogalactoside 161-199 immunglobulin heavy chain variable region Homo sapiens 60-64 9015295-3 1997 DNA encoding the scFv was sub-cloned into pDAP2 such that a scFv-alkaline phosphatase fusion protein was produced by transformed bacteria following induction by isopropyl-beta-D-thiogalactopyranoside (IPTG). Isopropyl Thiogalactoside 201-205 immunglobulin heavy chain variable region Homo sapiens 17-21 9015295-3 1997 DNA encoding the scFv was sub-cloned into pDAP2 such that a scFv-alkaline phosphatase fusion protein was produced by transformed bacteria following induction by isopropyl-beta-D-thiogalactopyranoside (IPTG). Isopropyl Thiogalactoside 201-205 immunglobulin heavy chain variable region Homo sapiens 60-64 11367724-8 1998 After induction of Lac promotor by 1 mM IPTG for 20 h, the scFv-M97 yield of HB2151 in SBA medium is about 2 micrograms/ml. Isopropyl Thiogalactoside 40-44 immunglobulin heavy chain variable region Homo sapiens 59-63 9373314-4 1996 RESULTS: Over 100 mg/l of the HNK20-scFv was produced in shake flasks after induction with isopropyl (beta-D-thiogalactopyranoside (IPTG). Isopropyl Thiogalactoside 132-136 immunglobulin heavy chain variable region Homo sapiens 36-40