PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11725000-5 1994 Similar phenomena were also observed, in that insulin regulated pRSVLTR-betagal and pMLSV(2)HBsAg in these three CHO lines. cho 113-116 insulin Cricetulus griseus 46-53 7508445-5 1994 A CHO cell line overexpressing the mutant insulin receptor, substituting Ala960 for Tyr960 and which was known to exhibit impaired tyrosine phosphorylation of IRS-1 and biological effects evoked by insulin, showed severely impaired insulin-dependent tyrosine phosphorylation of Shc and moderately impaired activation of Ras. cho 2-5 insulin Cricetulus griseus 42-49 7508445-5 1994 A CHO cell line overexpressing the mutant insulin receptor, substituting Ala960 for Tyr960 and which was known to exhibit impaired tyrosine phosphorylation of IRS-1 and biological effects evoked by insulin, showed severely impaired insulin-dependent tyrosine phosphorylation of Shc and moderately impaired activation of Ras. cho 2-5 insulin Cricetulus griseus 198-205 8011427-1 1994 We previously reported that Chinese hamster ovary (CHO) cell lines overexpressing mutated human insulin receptors (hIRs) in which the tyrosine residues 1162 and 1163 were replaced by phenylalanines (CHO-Y2) exhibited a marked defect in hormone-induced receptor internalization as compared to CHO transfectants overexpressing wild-type hIRs (CHO-R). cho 51-54 insulin Cricetulus griseus 96-103 8011427-1 1994 We previously reported that Chinese hamster ovary (CHO) cell lines overexpressing mutated human insulin receptors (hIRs) in which the tyrosine residues 1162 and 1163 were replaced by phenylalanines (CHO-Y2) exhibited a marked defect in hormone-induced receptor internalization as compared to CHO transfectants overexpressing wild-type hIRs (CHO-R). cho 199-202 insulin Cricetulus griseus 96-103 7686165-5 1993 In CHO cells, on the other hand, overexpression of either IGF-I or insulin receptors increased the sensitivity of thymidine incorporation to ligand, but maximal responsiveness was unchanged or decreased. cho 3-6 insulin Cricetulus griseus 67-74 8379190-5 1993 The GLUT2 content in the plasma membrane fraction of insulin-treated CHO cells expressing GLUT2 increased 3.8-fold compared to that of the control group. cho 69-72 insulin Cricetulus griseus 53-60 2250023-2 1990 We now provide evidence that insulin stimulation of myristoyl-diacylglycerol (DAG) production is also markedly impaired in CHO-Y2 cells; this is manifested as a decreased responsiveness and sensitivity to insulin as compared with CHO-R and parental CHO cells. cho 123-126 insulin Cricetulus griseus 29-36 1618780-6 1992 When CHO cells transfected with F/Y CT2 (CHO-F/Y CT2) were stimulated with insulin, autophosphorylation of the beta-subunit of the insulin receptor and the phosphorylation of an endogenous substrate (pp185) in the intact cell were normal compared with cells expressing HIR (CHO-HIR). cho 5-8 insulin Cricetulus griseus 75-82 1618780-6 1992 When CHO cells transfected with F/Y CT2 (CHO-F/Y CT2) were stimulated with insulin, autophosphorylation of the beta-subunit of the insulin receptor and the phosphorylation of an endogenous substrate (pp185) in the intact cell were normal compared with cells expressing HIR (CHO-HIR). cho 5-8 insulin Cricetulus griseus 131-138 1645354-6 1991 In the intact CHO cells, insulin-stimulated serine and threonine phosphorylation of the IR delta ct was reduced 20%, suggesting that most Ser/Thr phosphorylation sites are located outside of the C terminus. cho 14-17 insulin Cricetulus griseus 25-32 2250023-2 1990 We now provide evidence that insulin stimulation of myristoyl-diacylglycerol (DAG) production is also markedly impaired in CHO-Y2 cells; this is manifested as a decreased responsiveness and sensitivity to insulin as compared with CHO-R and parental CHO cells. cho 123-126 insulin Cricetulus griseus 205-212 2250023-2 1990 We now provide evidence that insulin stimulation of myristoyl-diacylglycerol (DAG) production is also markedly impaired in CHO-Y2 cells; this is manifested as a decreased responsiveness and sensitivity to insulin as compared with CHO-R and parental CHO cells. cho 230-233 insulin Cricetulus griseus 29-36 6391490-3 1984 Furthermore, two CHO cell glycosylation mutants, B4-2-1, lacking high mannose containing glycoproteins, and Lec 1.3c, lacking complex carbohydrate containing glycoproteins, bind insulin with a much higher and lower affinity respectively than wild type cells. cho 17-20 insulin Cricetulus griseus 178-185 2715185-6 1989 Glucose uptake in both CHO and CIR-0 was significantly enhanced by exogenous insulin, but not in Monr-31, MIR-2, and MIR-15. cho 23-26 insulin Cricetulus griseus 77-84 2715185-7 1989 The beta-subunits of insulin receptor in CHO, CIR-0, Monr-31, and MIR-2 were similarly phosphorylated. cho 41-44 insulin Cricetulus griseus 21-28 2842659-7 1987 The two mutant hIRs mediate very different physiological responses in transfected cells: the membrane-anchored, but not the cytosolic, hIR TPK mediates a constitutively elevated (135% the maximum insulin-stimulated response in CHO cells) insulin-independent uptake of 2-deoxyglucose. cho 227-230 insulin Cricetulus griseus 196-203 2842659-7 1987 The two mutant hIRs mediate very different physiological responses in transfected cells: the membrane-anchored, but not the cytosolic, hIR TPK mediates a constitutively elevated (135% the maximum insulin-stimulated response in CHO cells) insulin-independent uptake of 2-deoxyglucose. cho 227-230 insulin Cricetulus griseus 238-245 6371028-11 1984 The cellular uptake of 2-[3H]deoxyglucose into CHO cells was significantly enhanced in the presence of insulin, but only slight, if any, increase was observed in MonR cells. cho 47-50 insulin Cricetulus griseus 103-110 11340077-11 2001 Expression of Elk-1 converts CHO cells into a phenotype in which prolactin gene expression is increased by insulin treatment. cho 29-32 insulin Cricetulus griseus 107-114 9446605-3 1998 Insulin (10(-9)-10(-7) M) inhibited apoptosis induced by serum withdrawal in CHO-R cells in a concentration-dependent manner. cho 77-80 insulin Cricetulus griseus 0-7 287027-6 1979 When CHO cells are grown conventionally on plastic substrata or in suspension, insulin has little effect on cell growth at 4% serum concentration. cho 5-8 insulin Cricetulus griseus 79-86 19269599-7 2009 Simultaneous supplementation with insulin and bFGF synergistically promoted the growth of CHO cells and recombinant M-CSF synthesis in basal DMEM/F12 medium. cho 90-93 insulin Cricetulus griseus 34-41