PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 34009729-2 2021 CPPED1 is a recently identified serine/threonine protein phosphatase that dephosphorylates AKT1 of the PI3K-AKT signalling pathway. Serine 32-38 AKT serine/threonine kinase 1 Homo sapiens 91-95 33290747-8 2021 Using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, we found that miR-139 likely affected PC cell cycle by targeting RalB via the Ral/protein kinase B (Akt) serine/threonine kinase 1 (RAC)/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) pathway, thus affecting cell proliferation. Serine 191-197 AKT serine/threonine kinase 1 Homo sapiens 186-189 34009729-2 2021 CPPED1 is a recently identified serine/threonine protein phosphatase that dephosphorylates AKT1 of the PI3K-AKT signalling pathway. Serine 32-38 AKT serine/threonine kinase 1 Homo sapiens 91-94 33995085-1 2021 The serine/threonine kinase AKT is a key component of the PI3K/AKT/mTOR signaling pathway as it exerts a pivotal role in cell growth, proliferation, survival, and metabolism. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 33834942-1 2021 INTRODUCTION: : Akt is a widely-known serine threonine kinase involved in a series of critical cellular pathways like cell survival and proliferation. Serine 38-44 AKT serine/threonine kinase 1 Homo sapiens 16-19 33952452-2 2021 The phosphatidylinositol-3-kinase/AKT serine/threonine kinase/mammalian target of rapamycin (PI3K/AKT/mTOR) signalling pathway is frequently activated in colorectal cancer (CRC). Serine 38-44 AKT serine/threonine kinase 1 Homo sapiens 34-37 33995085-1 2021 The serine/threonine kinase AKT is a key component of the PI3K/AKT/mTOR signaling pathway as it exerts a pivotal role in cell growth, proliferation, survival, and metabolism. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 63-66 33660027-3 2021 We discovered that lithium enhanced glucose uptake by acute low-volume exercise-induced glycogen breakdown, which was facilitated by the dephosphorylation of serine 473-AKT (Ser473-AKT) and serine 9-GSK3beta. Serine 158-164 AKT serine/threonine kinase 1 Homo sapiens 169-172 33920446-1 2021 AKT, is a serine/threonine protein kinase comprising three isoforms-namely: AKT1, AKT2 and AKT3, whose inhibitors have been recognized as promising therapeutic targets for various human disorders, especially cancer. Serine 10-16 AKT serine/threonine kinase 1 Homo sapiens 0-3 33920446-1 2021 AKT, is a serine/threonine protein kinase comprising three isoforms-namely: AKT1, AKT2 and AKT3, whose inhibitors have been recognized as promising therapeutic targets for various human disorders, especially cancer. Serine 10-16 AKT serine/threonine kinase 1 Homo sapiens 76-80 33592378-6 2021 Upon observation of upstream regulators of epithelial-mesenchymal transition (EMT), levels of phosphorylated AKT (Ser-473 and Thr-308) and GSK-3beta (inactive form) were found to be decreased in TNNC1-KO cells. Serine 114-117 AKT serine/threonine kinase 1 Homo sapiens 109-112 33916378-4 2021 Preliminary mechanistic studies revealed that KA25 and KA39 exhibit time- and concentration-dependent inhibition of Akt Ser-473 phosphorylation. Serine 120-123 AKT serine/threonine kinase 1 Homo sapiens 116-119 33854635-6 2021 Mechanistically, SAA1 knockdown can inhibit serine/threonine protein kinase B (AKT) phosphorylation, thereby regulating the expression of apoptosis-related proteins such as Bcl2 and Bax, leading to GBM cell death. Serine 44-50 AKT serine/threonine kinase 1 Homo sapiens 79-82 33788710-2 2021 Along with the studies from our laboratory, it is well established that alpha-santalol targets the phosphatidylinositol-4,5-bisphosphate 3-kinase-AKT serine/ threonine kinase 1 (AKT) pathway to induce apoptosis but its growth-suppressive effects have not been fully elucidated. Serine 150-156 AKT serine/threonine kinase 1 Homo sapiens 146-149 33297755-9 2021 In primary human ECs, small interfering RNA-mediated TFEB knockdown blunts Akt (AKT serine/threonine kinase) signaling. Serine 84-90 AKT serine/threonine kinase 1 Homo sapiens 75-78 33635146-3 2021 Phosphorylation of beta-catenin at Ser 552 by AKT contributes to beta-catenin stability, transcriptional activity, and increase of cell proliferation. Serine 35-38 AKT serine/threonine kinase 1 Homo sapiens 46-49 33159506-8 2021 Western blot analysis was utilized to detect phosphatidylinositol-3 kinase/serine/threonine kinase (PI3K/AKT) signaling pathway-related protein expression and their phosphate expression levels. Serine 75-81 AKT serine/threonine kinase 1 Homo sapiens 105-108 33113244-6 2021 RNA interference of SP3 decreased active phosphorylation of Akt at serine and threonine sites. Serine 67-73 AKT serine/threonine kinase 1 Homo sapiens 60-63 33574926-3 2021 The present study found that the activity of the serine/threonine kinase Akt was negatively associated with H2A.X phosphorylated at the Ser16 site (H2A.X S16ph), but the mechanism of the inverse relationship remains elusive. Serine 49-55 AKT serine/threonine kinase 1 Homo sapiens 73-76 33574926-8 2021 The current study indicated that the serine/threonine kinase ribosomal S6 kinase 2 (RSK2) as a kinase of H2A.X could be phosphorylated by Akt at Ser19 site. Serine 37-43 AKT serine/threonine kinase 1 Homo sapiens 138-141 33670586-3 2021 The serine/threonine kinase AKT plays a crucial role in breast cancer bone metastasis but the effect of individual AKT isoforms remains unclear. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 33613259-2 2021 We have recently shown that the serine/threonine kinase Protein kinase N1 (PKN1) is a developmentally active regulator of cerebellar synaptic maturation by inhibiting AKT and the neurogenic transcription factor neurogenic differentiation factor-2 (NeuroD2). Serine 32-38 AKT serine/threonine kinase 1 Homo sapiens 167-170 33748278-9 2021 Y15 also downregulated the levels of p-FAK Tyr 397 and p-Akt Ser 473 in damaged external jugular vein and EA.hy926 cells. Serine 61-64 AKT serine/threonine kinase 1 Homo sapiens 57-60 33566837-6 2021 Second, MG53 gene knock-down in muscle cells does not lead to impaired insulin response as measured by Akt phosphorylation on Serine 473 and glucose uptake. Serine 126-132 AKT serine/threonine kinase 1 Homo sapiens 103-106 33297755-9 2021 In primary human ECs, small interfering RNA-mediated TFEB knockdown blunts Akt (AKT serine/threonine kinase) signaling. Serine 84-90 AKT serine/threonine kinase 1 Homo sapiens 80-83 33542904-1 2020 Serum and glucocorticoid-induced protein kinase 1 (SGK1) is a member of the "AGC" subfamily of protein kinases, which shares structural and functional similarities with the AKT family of kinases and displays serine/threonine kinase activity. Serine 208-214 AKT serine/threonine kinase 1 Homo sapiens 173-176 33152171-6 2021 Our data further revealed that DRP1 activation through serine 616 phosphorylation is regulated by ERK/AKT and CDK2 in lung adenocarcinoma cell lines. Serine 55-61 AKT serine/threonine kinase 1 Homo sapiens 102-105 33530625-2 2021 A double-negative feedback loop between two serine/threonine kinases AMPK (AMP-activated protein kinase) and Akt can regulate the adaptation of breast cancer cells to matrix-deprivation stress. Serine 44-50 AKT serine/threonine kinase 1 Homo sapiens 109-112 33190241-9 2021 Akt, a serine/threonine kinase is at the crossroad of cell survival, the progression of the cell cycle, cell signaling, cell growth, cell division, and inactivation of pro-apoptotic factors. Serine 7-13 AKT serine/threonine kinase 1 Homo sapiens 0-3 33181128-2 2021 The serine-threonine kinase Akt enhances survival and suppress apoptosis. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 33408715-3 2020 With this responsiveness, comes hastened apoptosis, due to a coupled mechanism of death and differentiation in both CD4+ and CD8+ lymphocytes via CD95 (Fas) and serine-threonine kinase (Akt). Serine 161-167 AKT serine/threonine kinase 1 Homo sapiens 186-189 33179291-5 2021 Phosphorylated Akt at serine 473 (p-Akt S473) expression was also evaluated by immunohistochemistry in mCRC patients. Serine 22-28 AKT serine/threonine kinase 1 Homo sapiens 15-18 33306168-1 2022 The serine/threonine p21-activated kinases (PAKs), as main effectors of the Rho GTPases Cdc42 and Rac, represent a group of important molecular switches linking the complex cytoskeletal networks to broad neural activity. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 98-101 30973040-4 2020 By immunohistochemical methods, we determined the expression of several factors [TGF-beta, Transforming Growth Factor B Receptor I (TGFBRI), TGFBRII, PI3K, AKT-Ser, AKT-Thr, mTOR, p-mTOR, inhibitor kB kinase (IKK), pIKK, inhibitor kB (IkB), pIkB, NF-kBp50, and NF-kBp65]. Serine 160-163 AKT serine/threonine kinase 1 Homo sapiens 156-159 33321953-5 2020 RTKs belong to the family of cell surface receptors that are activated by ligands which in turn activates two major downstream signaling pathways via Rapidly Accelerating Sarcoma/mitogen activated protein kinase/extracellular-signal-regulated kinase (Ras/MAPK/ERK) and phosphatidylinositol 3-kinase/a serine/threonine protein kinase/mammalian target of rapamycin (PI3K/AKT/mTOR). Serine 301-307 AKT serine/threonine kinase 1 Homo sapiens 369-372 33135306-11 2020 Co-immunoprecipitation (Co-IP) and computer-based molecular docking experiments demonstrated an interaction between Ac-SDKP or Ac-SDD KD P (Ac-SDKP/Ac-SDD KD P) and serine/arginine-rich protein-specific kinase 1 (SRPK1) that caused inhibition SRPK1-mediated phosphatidylinositol-3 kinase/ serine/threonine kinase (PIK3/AKT) signaling pathway activation and Smad2 phosphorylation and thereby attenuated lung fibrosis. Serine 165-171 AKT serine/threonine kinase 1 Homo sapiens 319-322 33334784-2 2020 Akt is a serine/threonine kinase which was originally discovered as an oncogene. Serine 9-15 AKT serine/threonine kinase 1 Homo sapiens 0-3 33227065-1 2020 AKT, a serine threonine kinase, exists in three different isoforms and is known for regulating several biological processes including tumorigenesis. Serine 7-13 AKT serine/threonine kinase 1 Homo sapiens 0-3 33253205-1 2020 Three AKT serine/threonine kinase isoforms (AKT1/AKT2/AKT3) mediate proliferation, metabolism, differentiation and anti-apoptotic signals. Serine 10-16 AKT serine/threonine kinase 1 Homo sapiens 6-9 33253205-1 2020 Three AKT serine/threonine kinase isoforms (AKT1/AKT2/AKT3) mediate proliferation, metabolism, differentiation and anti-apoptotic signals. Serine 10-16 AKT serine/threonine kinase 1 Homo sapiens 44-48 33253205-5 2020 In AKT, the HM is contiguous to the serine 473 and two other newly discovered (serine 477 and tyrosine 479) regulatory phosphorylation sites. Serine 36-42 AKT serine/threonine kinase 1 Homo sapiens 3-6 33253205-5 2020 In AKT, the HM is contiguous to the serine 473 and two other newly discovered (serine 477 and tyrosine 479) regulatory phosphorylation sites. Serine 79-85 AKT serine/threonine kinase 1 Homo sapiens 3-6 32780991-3 2020 This results in AKT serine/threonine kinase (Akt) activation, membrane focal adhesion turnover, and increased epithelial cell migration. Serine 20-26 AKT serine/threonine kinase 1 Homo sapiens 45-48 33179538-1 2020 Aim: The PHLDA (pleckstrin homology like domain, family A) gene family encodes proteins capable of inhibiting AKT (serine/threonine kinase) signaling through phosphoinositol binding competition. Serine 115-121 AKT serine/threonine kinase 1 Homo sapiens 110-113 32796067-15 2020 Interestingly, activation of the Akt family of serine/threonine protein kinases is linked to maintenance of latency. Serine 47-53 AKT serine/threonine kinase 1 Homo sapiens 33-36 32929041-0 2020 AKT Regulates NLRP3 Inflammasome Activation by Phosphorylating NLRP3 Serine 5. Serine 69-75 AKT serine/threonine kinase 1 Homo sapiens 0-3 33050597-3 2020 In the present study, we investigated the antimetastatic and anti-invasive effects of IMI in metastatic castration-resistant prostate cancer PC-3 cells, with an emphasis on the serine/threonine protein kinase AKT-mediated nuclear factor kappa B (NF-kappaB) signaling pathway. Serine 177-183 AKT serine/threonine kinase 1 Homo sapiens 209-212 33090513-5 2020 In addition, WS-PM2.5 accumulation in the cells induced the phosphorylation of serine/threonine protein kinase B (AKT) and nuclear factor kappa-light-chain enhancer of activated B cells (NFkappaB), as well as the expression of Kruppel-like factor 5 (KLF5). Serine 79-85 AKT serine/threonine kinase 1 Homo sapiens 114-117 33045011-1 2020 The serine/threonine protein kinase AKT is frequently over-activated in cancer and is associated with poor prognosis. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 36-39 32973139-4 2020 The binding of YB-1 with beta2-AR resulted in YB-1 phosphorylation at serine 102 (S102) via the beta-arrestin-1-dependent activation of the PI3K/AKT pathway, followed by the translocation of YB-1 to the nucleus to carry out its tumour-related function. Serine 70-76 AKT serine/threonine kinase 1 Homo sapiens 145-148 33123477-12 2020 Analysis of biological function revealed that the protein serine/threonine kinase activity and PI3K-AKT signaling pathway were the most significantly enriched functions and pathways, respectively. Serine 58-64 AKT serine/threonine kinase 1 Homo sapiens 100-103 33007920-10 2020 Thus, PI3K/AKT signaling was activated, inhibiting CHK1 activity via phosphorylation of its serine 280 residue preventing the repair of damaged DNA. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 11-14 32796067-4 2020 The Akt serine/threonine protein kinase family is linked to maintaining latency. Serine 8-14 AKT serine/threonine kinase 1 Homo sapiens 4-7 32937140-2 2020 The serine threonine kinase Akt plays a pivotal role in the control of cellular metabolism, survival, and mitogenic signaling. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 32948740-6 2020 The cyclin D1MEM was sufficient to induce phosphorylation of the serine threonine kinase Akt (Ser473) and augmented extranuclear localized 17beta-estradiol dendrimer conjugate (EDC)-mediated phosphorylation of Akt (Ser473). Serine 65-71 AKT serine/threonine kinase 1 Homo sapiens 89-92 32681941-8 2020 In terms of mechanism, 6-Gingerol potently suppressed phosphorylation of serine-threonine protein kinase (Akt) - mammalian target of rapamycin (mTOR) - signal transducer and activator of transcription 3 (STAT3) in LPS-treated microglia. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 106-109 32561413-3 2020 Although phosphorylation at serine (S) 166 site by AKT increases Mdm2 activity, phosphatases of Mdm2 have been still elusive. Serine 28-34 AKT serine/threonine kinase 1 Homo sapiens 51-54 32895368-11 2020 Taken together, our study demonstrated that LINC01559 accelerated GC progression via upregulating PGK1 and downregulating PTEN to trigger phosphatidylinositol 3-kinase/AKT serine/threonine kinase (PI3K/AKT) pathway, indicating LINC01559 as a potential biomarker for GC treatment. Serine 172-178 AKT serine/threonine kinase 1 Homo sapiens 168-171 32899862-3 2020 Sustained HSP25 expression also enhances AKT-serine (S) 473 phosphorylation leading to astroglial autophagy via glycogen synthase kinase-3beta/bax interacting factor 1 signaling pathway. Serine 45-51 AKT serine/threonine kinase 1 Homo sapiens 41-44 32561413-3 2020 Although phosphorylation at serine (S) 166 site by AKT increases Mdm2 activity, phosphatases of Mdm2 have been still elusive. Serine 36-37 AKT serine/threonine kinase 1 Homo sapiens 51-54 32493823-12 2020 Because monocytes are short-lived cells, HCMV must subvert the natural short lifespan of these blood cells by inducing a distinct activation of Akt, a serine/theonine-protein kinase. Serine 151-157 AKT serine/threonine kinase 1 Homo sapiens 144-147 32842547-7 2020 Several clinical trials for recurrent or advanced ECs have been attempted using PI3K-serine/threonine kinase (AKT) inhibitors. Serine 85-91 AKT serine/threonine kinase 1 Homo sapiens 110-113 32781572-0 2020 Brain-Specific Serine-47 Modification of Cytochrome c Regulates Cytochrome c Oxidase Activity Attenuating ROS Production and Cell Death: Implications for Ischemia/Reperfusion Injury and Akt Signaling. Serine 15-21 AKT serine/threonine kinase 1 Homo sapiens 186-189 32641498-9 2020 Activation of the serine threonine kinase Akt is required for Reelin-dependent dendritic growth and we find that CSPGs induce Akt dephosphorylation, an effect that can be counteracted by Reelin addition. Serine 18-24 AKT serine/threonine kinase 1 Homo sapiens 42-45 32641498-9 2020 Activation of the serine threonine kinase Akt is required for Reelin-dependent dendritic growth and we find that CSPGs induce Akt dephosphorylation, an effect that can be counteracted by Reelin addition. Serine 18-24 AKT serine/threonine kinase 1 Homo sapiens 126-129 32317083-0 2020 Serine 389 phosphorylation of 3-phosphoinositide-dependent kinase 1 by UNC-51-like kinase 1 affects its ability to regulate Akt and p70 S6kinase. Serine 0-6 AKT serine/threonine kinase 1 Homo sapiens 124-127 32627660-7 2021 ABBREVIATIONS: AKT1/PKB: AKT serine/threonine kinase 1; ATG: autophagy related; BECN1: beclin 1; CGAS: cyclic GMP-AMP synthase; EIF2AK4/GCN2: eukaryotic translation initiation factor 2 alpha kinase 4; ER: endoplasmic reticulum; FFAR: free fatty acid receptor; GABARAP: GABA type A receptor-associated protein; IFN: interferon; IL: interleukin; LAP: LC3-associated phagocytosis; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAP3K7/TAK1: mitogen-activated protein kinase kinase kinase 7; MAPK: mitogen-activated protein kinase; MTOR: mechanistic target of rapamycin kinase; NLR: NOD (nucleotide-binding oligomerization domain) and leucine-rich repeat containing proteins; PI3K, phosphoinositide 3-kinase; PRR: pattern-recognition receptor; PtdIns3K: phosphatidylinositol 3-kinase; RALB: RAS like proto-oncogene B; RHEB: Ras homolog, MTORC1 binding; RIPK1: receptor interacting serine/threonine kinase 1; RRAG: Ras related GTP binding; SQSTM1/p62: sequestosome 1; STING1/TMEM173: stimulator of interferon response cGAMP interactor 1; STK11/LKB1: serine/threonine kinase 11; TBK1: TANK binding kinase 1; TLR: toll like receptor; TNF: tumor necrosis factor; TRAF6: TNF receptor associated factor 6; TRIM: tripartite motif protein; ULK1: unc-51 like autophagy activating kinase 1; V-ATPase: vacuolar-type H+-proton-translocating ATPase. Serine 29-35 AKT serine/threonine kinase 1 Homo sapiens 15-19 32842445-5 2020 The effects of different concentrations of Tectoridin on the protein levels of phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT) signaling pathway were detected by western blot assay. Serine 112-118 AKT serine/threonine kinase 1 Homo sapiens 137-140 32760285-9 2020 In addition, PPARdelta activation recovered EtOH-induced loss of protein kinase B (AKT) phosphorylation at serine 473 via rapamycin-insensitive companion of mammalian target of rapamycin (Rictor) activation. Serine 107-113 AKT serine/threonine kinase 1 Homo sapiens 83-86 32383528-4 2020 The ZMF absolute enhanced the phosphorylation levels of serine/threonine-specific protein kinase (Akt), p38 mitogen-activated protein kinase (MAPK), and extracellular signal-regulated kinase1/2 in HaCaTs. Serine 56-62 AKT serine/threonine kinase 1 Homo sapiens 98-101 32350110-3 2020 AKT1 is itself activated by phosphorylation at Thr-308 and Ser-473. Serine 59-62 AKT serine/threonine kinase 1 Homo sapiens 0-4 32350110-9 2020 Compared with pAKT1Thr-308, the addition of Ser-473 phosphorylation increased AKT1 activities on some, but not all of its substrates. Serine 44-47 AKT serine/threonine kinase 1 Homo sapiens 15-19 30270668-5 2020 Among these serine/threonine kinases, PIM kinases have garnered much attention as they not only affect insulin sensitivity by phosphorylating IRSs directly and/or indirectly but also alter the activities of their downstream molecules like PI3K, AKT, and mTOR. Serine 12-18 AKT serine/threonine kinase 1 Homo sapiens 245-248 32545311-4 2020 Proliferation is upregulated through two mechanisms: (1) ATP binding to the G-protein-coupled receptor P2Y2, commencing a kinase signaling cascade that activates the serine-threonine kinase Akt, and (2) the transactivation of the epidermal growth factor receptor (EGFR), leading to a series of protein signals that activate the extracellular signal-regulated kinases (ERK) 1/2. Serine 166-172 AKT serine/threonine kinase 1 Homo sapiens 190-193 32347294-2 2020 mTORC1 phosphorylates S6K1 at Thr 389, whereas mTORC2 phosphorylates AKT at Ser 473 to promote cell growth. Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 69-72 32455838-6 2020 DAGs activate protein kinase C that inhibit insulin-PI3K-Akt signaling by phosphorylating serine residues on insulin receptor substrate (IRS). Serine 90-96 AKT serine/threonine kinase 1 Homo sapiens 57-60 32375768-10 2020 Additionally, circBFAR overexpression increased the expression of MET and activated downstream phosphorylation of Akt (Ser 473) and further activated the MET/PI3K/Akt signaling pathway, which ultimately promoted the progression of PDAC cells. Serine 119-122 AKT serine/threonine kinase 1 Homo sapiens 114-117 32312752-6 2020 Further analysis revealed that manassantin B specifically blocks mTOR complex 2 (mTORC2)-mediated phosphorylation of AKT Ser/Thr protein kinase at Ser-473 and protein kinase Calpha (PKCalpha) at Ser-657. Serine 121-124 AKT serine/threonine kinase 1 Homo sapiens 117-120 32312752-6 2020 Further analysis revealed that manassantin B specifically blocks mTOR complex 2 (mTORC2)-mediated phosphorylation of AKT Ser/Thr protein kinase at Ser-473 and protein kinase Calpha (PKCalpha) at Ser-657. Serine 147-150 AKT serine/threonine kinase 1 Homo sapiens 117-120 32410999-4 2020 Alkylphosphocholine (APC) derivatives represent a novel class of antineoplastic agents that inhibit the serine-threonine kinase Akt (i.e. protein kinase B), which mediates cell survival and cause cell cycle arrest. Serine 104-110 AKT serine/threonine kinase 1 Homo sapiens 128-131 31889370-9 2020 In addition, we observed that CCN5 knockdown increased the expression levels of PI3K (p85alpha and p110alpha) and phosphorylated AKT at serine 473 (p-AKT Ser473) in Tca-8113 cells. Serine 136-142 AKT serine/threonine kinase 1 Homo sapiens 129-132 32395524-8 2020 Dezocine has diverse effects on aerobic glycolysis and adjusts the serine/threonine kinase 1 (Akt1)-glycogen synthase kinase-3beta (GSK-3beta) pathway. Serine 67-73 AKT serine/threonine kinase 1 Homo sapiens 94-98 32183046-9 2020 Activation of the angiogenic factor with G patch and FHA domains 1 (AGGF1) caused enhanced phosphorylation of serine/threonine kinase (Akt) and extracellular regulated protein kinases (Erk1/2). Serine 110-116 AKT serine/threonine kinase 1 Homo sapiens 135-138 31879363-2 2020 Nuclear translocation of YBX1 is facilitated by YBX1 phosphorylation at serine 102 by AKT, p70S6K, and p90RSK, and the phosphorylated YBX1 (pYBX1) promotes expression of genes related to drug resistance and cell growth. Serine 72-78 AKT serine/threonine kinase 1 Homo sapiens 86-89 30798436-3 2020 Herein, in silico structure- and ligand-based approaches have been applied to screen in-house IIIM natural product repository for Akt1 (serine/threonine protein kinases) which is a well-known therapeutic target for cancer due to its overexpression and preventing the cells from undergoing apoptosis. Serine 136-142 AKT serine/threonine kinase 1 Homo sapiens 130-134 31917333-7 2020 Interestingly, acute exposure to both 2AG and Delta9-THC inhibited phosphorylation of serine/threonine kinase extracellular signal-regulated protein kinases (ERK1/2), whereas Delta9-THC also reduced phosphorylation of Akt (aka PKB). Serine 86-92 AKT serine/threonine kinase 1 Homo sapiens 218-221 31917333-7 2020 Interestingly, acute exposure to both 2AG and Delta9-THC inhibited phosphorylation of serine/threonine kinase extracellular signal-regulated protein kinases (ERK1/2), whereas Delta9-THC also reduced phosphorylation of Akt (aka PKB). Serine 86-92 AKT serine/threonine kinase 1 Homo sapiens 227-230 32020212-10 2020 Finally, it was observed that TGM3 inhibited epithelial-to-mesenchymal transition and activated phosphorylated AKT serine/threonine kinase in CRC cells. Serine 115-121 AKT serine/threonine kinase 1 Homo sapiens 111-114 31215069-5 2020 Knockdown of Nqo1 enhanced activity of the serine/threonine phosphatase, protein phosphatase 2A, which operates at the intersection of the PI3K/Akt and MAPK/ERK pathways and dephosphorylates and inactivates pyruvate dehydrogenase kinase 1, Akt, Raf, mitogen-activated protein kinase kinase, and ERK1/2. Serine 43-49 AKT serine/threonine kinase 1 Homo sapiens 144-147 31215069-5 2020 Knockdown of Nqo1 enhanced activity of the serine/threonine phosphatase, protein phosphatase 2A, which operates at the intersection of the PI3K/Akt and MAPK/ERK pathways and dephosphorylates and inactivates pyruvate dehydrogenase kinase 1, Akt, Raf, mitogen-activated protein kinase kinase, and ERK1/2. Serine 43-49 AKT serine/threonine kinase 1 Homo sapiens 240-243 31237701-4 2020 The most enriched phosphatidylinositol 3" kinase-serine/threonine kinase (PI3K-Akt) signaling pathway stimulated and directly regulated cellular metabolism by priming glucose aerobic glycolysis, arginine and proline metabolism, glutathione metabolism, and arachidonic acid metabolism during adipogenesis, targeting the potential key genes, such as fatty acid synthase (FABP4), phosphoenolpyruvate carboxykinase 1 (PKC1), stearoyl-CoA desaturase (SCD), and solute carrier family 2 member 1 of Gluts (SLC2A1). Serine 49-55 AKT serine/threonine kinase 1 Homo sapiens 79-82 31810747-1 2020 AKT1 encodes a serine/threonine kinase that has as one of its best-known substrates glycogen synthase kinase-3 (GSK3), a primary target for lithium. Serine 15-21 AKT serine/threonine kinase 1 Homo sapiens 0-4 31902918-6 2020 Furthermore, metformin also enhanced autophagic flux, inhibited the phosphorylation of the serine/threonine protein kinase (AKT)/mammalian target of rapamycin (mTOR), mitogen-activated protein kinases (MAPKs) related protein levels and the level of miR-221 in LPS-stimulated RAW264.7 cells. Serine 91-97 AKT serine/threonine kinase 1 Homo sapiens 124-127 32741948-8 2020 Mechanistic studies revealed that GN lowers protein levels of phospho-PI3K (p85 tyrosine (Tyr)458), phospho-Akt (serine (Ser)473), and Akt downstream molecules Mcl-1 in HepG2 and HCCLM3 cells. Serine 113-119 AKT serine/threonine kinase 1 Homo sapiens 108-111 32741948-8 2020 Mechanistic studies revealed that GN lowers protein levels of phospho-PI3K (p85 tyrosine (Tyr)458), phospho-Akt (serine (Ser)473), and Akt downstream molecules Mcl-1 in HepG2 and HCCLM3 cells. Serine 121-124 AKT serine/threonine kinase 1 Homo sapiens 108-111 31693862-2 2019 Specific molecules, such as mammalian target of rapamycin (mTOR) and the serine/threonine kinase Akt (AKT), have been proven to be important for PF activation. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 97-100 32120136-1 2020 The serine/threonine kinase AKT, also known as protein kinase B (PKB), is the major substrate to phosphoinositide 3-kinase (PI3K) and consists of three paralogs: AKT1 (PKBalpha), AKT2 (PKBbeta) and AKT3 (PKBgamma). Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 32120136-1 2020 The serine/threonine kinase AKT, also known as protein kinase B (PKB), is the major substrate to phosphoinositide 3-kinase (PI3K) and consists of three paralogs: AKT1 (PKBalpha), AKT2 (PKBbeta) and AKT3 (PKBgamma). Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 162-166 32120136-1 2020 The serine/threonine kinase AKT, also known as protein kinase B (PKB), is the major substrate to phosphoinositide 3-kinase (PI3K) and consists of three paralogs: AKT1 (PKBalpha), AKT2 (PKBbeta) and AKT3 (PKBgamma). Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 168-176 31693862-2 2019 Specific molecules, such as mammalian target of rapamycin (mTOR) and the serine/threonine kinase Akt (AKT), have been proven to be important for PF activation. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 102-105 31842576-10 2019 One of the intracellular signalling mechanism includes the activity of phosphatidylinositol-3-kinase (phosphoinositide 3-kinase) (PI3K) - protein kinase B (serine-threonine protein kinase Akt) (Akt) pathway. Serine 156-162 AKT serine/threonine kinase 1 Homo sapiens 188-191 31842576-10 2019 One of the intracellular signalling mechanism includes the activity of phosphatidylinositol-3-kinase (phosphoinositide 3-kinase) (PI3K) - protein kinase B (serine-threonine protein kinase Akt) (Akt) pathway. Serine 156-162 AKT serine/threonine kinase 1 Homo sapiens 194-197 31824825-7 2019 Western blot analyses revealed that EGM preconditioning with gelatin coating induced the expression of endothelial nitric oxide synthase (eNOS), a mature EC-specific marker, as well as phosphorylated Akt at serine 473, a signaling molecule related to eNOS activation. Serine 207-213 AKT serine/threonine kinase 1 Homo sapiens 200-203 31173856-1 2019 Akt is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Serine 9-15 AKT serine/threonine kinase 1 Homo sapiens 0-3 31542232-8 2019 We further showed that ectopically expressed USP46 obviously inhibited the AKT signaling pathway in cancer cells, while USP46 depletion caused a dramatic increase in AKT activity reflected by phosphorylation in the serine and threonine residues of AKT or downstream p70S6K1. Serine 215-221 AKT serine/threonine kinase 1 Homo sapiens 166-169 31542232-8 2019 We further showed that ectopically expressed USP46 obviously inhibited the AKT signaling pathway in cancer cells, while USP46 depletion caused a dramatic increase in AKT activity reflected by phosphorylation in the serine and threonine residues of AKT or downstream p70S6K1. Serine 215-221 AKT serine/threonine kinase 1 Homo sapiens 166-169 31564197-5 2019 Hence, the TCL1-dependent enhancement of serine/threonine AKT/PKB kinase activity favoring cell proliferation appears to be associated to the promotion of glucose transport and activation of glycolytic pathways. Serine 41-47 AKT serine/threonine kinase 1 Homo sapiens 58-65 31341021-8 2019 Mechanistically, shear stress stimulated activation of AKT Ser/Thr kinase 1 (AKT1), leading to phosphorylation of IGPR-1 at Ser-220. Serine 59-62 AKT serine/threonine kinase 1 Homo sapiens 77-81 31624287-4 2019 SM decreased the phosphorylation of Akt at Ser 473, which was mediated by mTOR complex 2 (mTORC2), in C2C12 myoblasts, leading to a decrease in the cell growth rate. Serine 43-46 AKT serine/threonine kinase 1 Homo sapiens 36-39 31624287-6 2019 In addition, SM increased the phospholipase D (PLD) activity by enhancing PLD2 expression, resulting in the dissociation of mSIN1 from the mTORC2, followed by decrease in the phosphorylation of Akt at Ser 473, and FOXO1 at Ser 256 in C2C12 myoblasts. Serine 201-204 AKT serine/threonine kinase 1 Homo sapiens 194-197 30721374-8 2019 In addition, L-serine reversed cellular insulin resistance determined by insulin-induced phosphorylation of Akt and GLUT4 expression and membrane translocation. Serine 15-21 AKT serine/threonine kinase 1 Homo sapiens 108-111 31399647-11 2019 Furthermore, AKT-mediated Thr-150, Ser-224, Thr-234, and Thr-262 phosphorylation promoted lung cancer cell growth and metastasis in vitro and in vivo. Serine 35-38 AKT serine/threonine kinase 1 Homo sapiens 13-16 31262199-11 2019 Abbreviations: AKT1: serine-threonine protein kinase 1; AMPK: 5" AMP-activated protein kinase; CAT: catalase; CON: control; FBS: fasting blood sugar; GLUT1: glucose transporter 1; GSH: reduced glutathione; GSSG: Glutathione disulfide; HE: hematoxylin and eosin stains; HDL: high-density lipoprotein; HFD: high fat diet; IL-6: interleukin-6; K18: keratin 18; LD: lactation day; LDL: low-density lipoprotein; LKB1: serine-threonine liver kinase B1; MDA: malondialdehyde; mTOR: the mammalian target of rapamycin; NAD: nicotinamide adenine dinucleotide; NADH: nicotinamide adenine dinucleotide phosphate; NS: nigella sativa; PBS: phosphate-buffered saline; PGC1alpha: peroxisome proliferator-activated receptor gamma coactivator 1-alpha; SIRT1: sirtuin 1; SOD: superoxide dismutase; T-AOC: total antioxidants; TFAM: transcription factor A mitochondrial; TG: triglycerides; TNF-alpha: tumor necrosis factor-alpha; TQ: thymoquinone; TQ10: high fat diet + thymoquinone 10% ppm; TQ20: high fat diet + thymoquinone 20% ppm; UCP2: uncoupling Protein 2. Serine 21-27 AKT serine/threonine kinase 1 Homo sapiens 15-19 30939964-12 2019 Abbreviations: 4HPR: 4-hydroxy(phenyl)retinamide; AKT: AKT1 serine/threonine kinase 1; ALS: amyotrophic lateral sclerosis; ATG: autophagy related; AVs: autophagic vesicle; C9orf72: chromosome 9 open reading frame 72; CASP3: caspase 3; CHAT: choline O-acetyltransferase; CYCS: cytochrome c, somatic; DIV: day in vitro; FTD: frontotemporal dementia; FUS: FUS RNA binding protein; GFP: green fluorescent protein; hiPSCs: human induced pluripotent stem cells; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MNs: motoneurons; mRFP: monomeric red fluorescent protein; MTOR: mechanistic target of rapamycin kinase; NFE2L2/NRF2: nuclear factor, erythroid 2 like 2; RARA: retinoic acid receptor alpha; SLC18A3/VACHT: solute carrier family 18 (vesicular acetylcholine transporter), member 3; SQSTM1/p62: sequestosome 1; TBK1: TANK binding kinase 1; TEM: transmission electron microscopy. Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 50-53 31540244-5 2019 AKT protein expression and activation (as measured by phosphorylation of serine 473) were differentially regulated in E-cadherin-null MCF10A and NCI-N87 cells, with downregulation in the normal breast cells, but upregulation in the gastric cancer cells. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 0-3 30680530-5 2019 Ectopic Rsu1 inhibited AKT1 phosphorylation while Rsu1 depletion induced AKT activation and AKT1 phosphorylation of MKK4 on serine 80, blocking MKK4 activity. Serine 124-130 AKT serine/threonine kinase 1 Homo sapiens 92-96 31502574-5 2019 Meanwhile a western blot assay showed that c-Met together with matrix metalloproteinases-9 (MMP9) and phosphorylation of the serine/threonine kinase AKT (p-AKT) was significantly down-regulated by calycosin. Serine 125-131 AKT serine/threonine kinase 1 Homo sapiens 149-152 31502574-5 2019 Meanwhile a western blot assay showed that c-Met together with matrix metalloproteinases-9 (MMP9) and phosphorylation of the serine/threonine kinase AKT (p-AKT) was significantly down-regulated by calycosin. Serine 125-131 AKT serine/threonine kinase 1 Homo sapiens 154-159 31308176-3 2019 In response to growth factors, the serine/threonine kinase AKT phosphorylates Thr24 and Ser256 in FOXO1 to stimulate binding of 14-3-3 proteins, causing FOXO1 inactivation. Serine 35-41 AKT serine/threonine kinase 1 Homo sapiens 59-62 31017040-10 2019 EVs also increased phosphorylation of Akt and its downstream target GSK3beta (Ser 9) indicating that PI3K/Akt signaling pathway may act as suppressor of NFkappaB activity. Serine 78-81 AKT serine/threonine kinase 1 Homo sapiens 38-41 31173266-9 2019 As indicated by western blot analysis, the mitogen-activated protein kinase (MAPK)/extracellular-signal-regulated kinase (ERK) signaling pathway and the phosphatidylinositol 3-kinase (PI3K)/AKT serine/threonine kinase (AKT) signaling pathway was regulated by PTPN1, while knockdown of PTPN1 significantly suppressed the MAPK/ERK and PI3K/AKT pathways, in addition to the downstream oncogenic transcription factor MYC Proto-Oncogene. Serine 194-200 AKT serine/threonine kinase 1 Homo sapiens 190-193 31467925-7 2019 We found that SFN decreased HG-induced production of reactive oxygen species (ROS), phosphorylation of PI3K/Akt at serine 473, and inhibitory phosphorylation of serine/threonine kinase glycogen synthase kinase-3beta (GSK-3beta) at serine 9. Serine 115-121 AKT serine/threonine kinase 1 Homo sapiens 108-111 30734931-5 2019 We provide evidence using primary cortical cultured neurons from Pink1-deficient mice that Pink1 increases AKT phosphorylation at Serine 473 (Ser473) challenged by 1-methyl-4-phenylpyridinium (MPP+ ) and that over-expression of FKBP5 using an adeno-associated virus delivery system negatively regulates AKT phosphorylation at Ser473 in murine-cultured cortical neurons. Serine 130-136 AKT serine/threonine kinase 1 Homo sapiens 303-306 31134093-3 2019 These neutrophil alterations include marked defects in intracellular signaling pathways involving serine/threonine kinases such as protein kinase B (AKT), p38-mitogen-activated protein kinase (MAPK), and the MAP kinases1/2; activation of the NADPH oxidase complex; myeloperoxidase (MPO) release; and bactericidal activity of neutrophils stimulated by the bacterial peptide formyl-Methionine-Leucine-Phenylalanine (fMLF). Serine 98-104 AKT serine/threonine kinase 1 Homo sapiens 149-152 31082618-0 2019 Metformin inhibits beta-catenin phosphorylation on Ser-552 through an AMPK/PI3K/Akt pathway in colorectal cancer cells. Serine 51-54 AKT serine/threonine kinase 1 Homo sapiens 80-83 31113871-7 2019 Furthermore, mechanistic investigations showed that the AKT (AKT serine/threonine kinase) pathway can regulate the effects of NPTX1 in HCC cells. Serine 65-71 AKT serine/threonine kinase 1 Homo sapiens 56-59 31150511-8 2019 Via its interaction and inhibition of ROR1, strictinin reduced AKT phosphorylation on ser-473, inhibiting downstream phosphorylation and inhibition of GSK3beta. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 63-66 31059711-7 2019 Decreased microRNA (miR)-144 expression and increased PTEN expression after TPL treatment were demonstrated, and TPL-enhanced p85alpha-PTEN complexes and inhibitory effects on Akt (Ser 473) phosphorylation and S phase arrest were suppressed by ectopic PTEN short hairpin RNA or miR-144 expression. Serine 181-184 AKT serine/threonine kinase 1 Homo sapiens 176-179 31136202-8 2019 Adipocyte insulin signaling (p-AKT at Ser-473 with 1.2 nM insulin) increased in HT (Pre: 0.29 +- 0.14, Post: 0.93 +- 0.29 AU; P = 0.021). Serine 38-41 AKT serine/threonine kinase 1 Homo sapiens 31-34 31056260-10 2019 Importantly, CDDP combined with LY294002, inhibitor of phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase (AKT) signaling, markedly decreased the expression of NRF2, HO-1, NQO1 and GCLC in drug-resistant cervical cancer cells. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 88-91 30986276-6 2019 On the other hand, chronic beta2-AR stimulation in masseter muscle induced muscle hypertrophy without histological abnormalities, as in the case of cardiac muscle, concomitantly with phosphorylation of Akt (Ser-473) and mTOR (Ser-2448) and increased expression of microtubule-associated protein light chain 3-II, an autophagosome marker. Serine 207-210 AKT serine/threonine kinase 1 Homo sapiens 202-205 31035650-7 2019 p85alpha-phosphatase and tensin homolog deleted from chromosome 10 (PTEN) complex-mediated inactivation of Akt was required for citrate-induced G2/M phase cell cycle arrest because PTEN short hairpin RNA or a PTEN inhibitor (SF1670) blocked the suppression of Akt Ser 473 phosphorylation and the induction of cyclin B1-p-CDK1 (Thr 161) complexes and G2/M phase arrest by citrate. Serine 264-267 AKT serine/threonine kinase 1 Homo sapiens 107-110 30992506-6 2019 Results showed that there is clear upregulation of serine 473 phosphorylation level of AKT in NS5A transfected cells as compared with control (without NS5A). Serine 51-57 AKT serine/threonine kinase 1 Homo sapiens 87-90 30992506-7 2019 In conclusion, upregulation of serine 473 phosphorylation by NS5A of HCV genotype 3a suggests that this gene impairs the normal insulin AKT/PKB signaling pathway that leads towards insulin resistance and Type 2 diabetes mellitus. Serine 31-37 AKT serine/threonine kinase 1 Homo sapiens 136-143 30381079-6 2019 Further studies suggested that serine and threonine residues, but not lysines, in OPB play a key role in YY1-AKT interaction. Serine 31-37 AKT serine/threonine kinase 1 Homo sapiens 109-112 30971932-10 2019 In addition, GGF2 treatment enhanced Akt phosphorylation (at both threonine and serine sites, by 75 and 139%, respectively, P = 0.029 and P = 0.01), which was blunted by ErbB 2/4 receptor blockade. Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 37-40 30846598-3 2019 Akt phosphorylates NAD kinase (NADK), the sole cytosolic enzyme that catalyzes the synthesis of NADP+ from NAD+ (the oxidized form of NADH), on three serine residues (Ser44, Ser46, and Ser48) within an amino-terminal domain. Serine 150-156 AKT serine/threonine kinase 1 Homo sapiens 0-3 30290714-11 2019 Abbreviations: AD: Alzheimer disease; AEC: airway epithelial cells; BALF: bronchoalveolar lavage fluid; AKT: AKT serine/threonine kinase; CALCOCO2/NDP52: calcium binding and coiled-coil domain 2; CDKN1A: cyclin dependent kinase inhibitor 1A; CDKN2A: cyclin dependent kinase inhibitor 2A; COPD: chronic obstructive pulmonary disease; CS: cigarette smoke; CSE: CS extract; CXCL1: C-X-C motif chemokine ligand 1; CXCL8: C-X-C motif chemokine ligand 8; HBEC: human bronchial epithelial cells; 4-HNE: 4-hydroxynonenal; IL: interleukin; KO: knockout; LF: lung fibroblasts; LPS: lipopolysaccharide; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MTOR: mechanistic target of rapamycin kinase; 8-OHdG: 8-hydroxy-2"-deoxyguanosine; OPTN: optineurin; PRKN: parkin RBR E3 ubiquitin protein ligase; PCD: programmed cell death; PFD: pirfenidone; PIK3C: phosphatidylinositol-4:5-bisphosphate 3-kinase catalytic subunit; PINK1: PTEN induced putative kinase 1; PTEN: phosphatase and tensin homolog; RA: rheumatoid arthritis; ROS: reactive oxygen species; SA-GLB1/beta-Gal: senescence-associated-galactosidase, beta 1; SASP: senescence-associated secretory phenotype; SNP: single nucleotide polymorphism; TNF: tumor necrosis factor. Serine 113-119 AKT serine/threonine kinase 1 Homo sapiens 104-107 30688659-8 2019 It also activated mTORC2 to phosphorylate AKT at serine 473 for full activation. Serine 49-55 AKT serine/threonine kinase 1 Homo sapiens 42-45 30535469-3 2019 Threonine and serine phosphorylation contribute to Akt translocation from the cytoplasm to the nucleus and further mediates enzymatic biological effects, including those involved in cell proliferation, apoptosis inhibition, cell migration, vesicle transport and cell cancerous transformation. Serine 14-20 AKT serine/threonine kinase 1 Homo sapiens 51-54 30535469-8 2019 Inhibition of glycogen synthase kinase 3beta activity by phosphorylation of its N-terminal serine increases accumulation of cyclin D1, which promotes the cell cycle and improves cell proliferation through the PI3K/Akt signaling pathway. Serine 91-97 AKT serine/threonine kinase 1 Homo sapiens 214-217 30169424-9 2019 Furthermore, bacillomycin D-C16 effectively repressed phosphorylation of the serine-threonine protein kinase Akt at Ser-473 and increased the levels of the FoxO3a protein. Serine 116-119 AKT serine/threonine kinase 1 Homo sapiens 109-112 30881497-10 2019 The PPI network revealed that these genes were involved in significant pathways, including "ECM organization" signaling pathways (Hippo signaling pathway, TGF-beta signaling pathway, Hedgehog signaling pathway and Wnt signaling pathway), "serine-type peptidase activity" signaling pathway (PI3K-Akt signaling pathway, TNF-alpha signaling pathway and Wnt signaling pathway) and "extracellular region" signaling pathways (RTP signaling pathway, G protein-coupled receptor signaling pathway and RAS-RAF-MAPK signaling pathway). Serine 239-245 AKT serine/threonine kinase 1 Homo sapiens 295-298 30536958-2 2019 Functional HSP90 is required for the stability of AKT, a serine-threonine kinase phosphorylated in response to growth factor stimulation. Serine 57-63 AKT serine/threonine kinase 1 Homo sapiens 50-53 30650200-6 2019 Further, OSI-027 almost completely blocked the phosphorylation of the mTORC1 substrates, S6K1, S6 and 4EBP1, and the mTORC2 substrate, AKT, at Ser-473. Serine 143-146 AKT serine/threonine kinase 1 Homo sapiens 135-138 30662561-12 2019 Mechanistic investigations showed that OTUB2 stimulated the Warburg effect and induced the activation of the serine/threonine kinase/mechanistic target of rapamycin kinase (AKT/mTOR) pathway in different NSCLC cells. Serine 109-115 AKT serine/threonine kinase 1 Homo sapiens 173-176 30518934-0 2018 LncRNA DANCR upregulates PI3K/AKT signaling through activating serine phosphorylation of RXRA. Serine 63-69 AKT serine/threonine kinase 1 Homo sapiens 30-33 30359271-14 2018 FBXO17 knockdown reduced Akt Ser 473 phosphorylation approaching statistical significance with no effect on Thr 308. Serine 29-32 AKT serine/threonine kinase 1 Homo sapiens 25-28 30571167-4 2018 Approach and Results- The phosphorylation of the mTORC2 target Akt at S473 (serine 473) was significantly elevated in platelets from the distal end of left anterior descending obstructions from patients who underwent off-pump coronary artery bypass grafting compared with platelets from healthy subjects. Serine 76-82 AKT serine/threonine kinase 1 Homo sapiens 63-66 29769704-7 2018 RESULTS: PCA restored insulin-induced phosphorylation in OB-VAT by increasing phospho-Tyr-IRS-1 and phospho-Ser-Akt after insulin stimulation as observed in NW-VAT (p < 0.05). Serine 108-111 AKT serine/threonine kinase 1 Homo sapiens 112-115 30042494-5 2018 Here, we demonstrated that the mtROS stimulated phosphatidylinositol 3 kinase/AKT/mammalian target of rapamycin (mTOR) cascade, and the activated mTORC1 subsequently induced autophagic signaling via phosphorylation of uncoordinated-51-like kinase 1 (ULK1) at serine 317 and upregulation of Atg proteins to prompt muscle differentiation. Serine 259-265 AKT serine/threonine kinase 1 Homo sapiens 78-81 30143532-5 2018 Sequence analysis identified a putative site for both Akt-mediated phosphorylation and 14-3-3 binding at Ser-124, and we found that Akt phosphorylates TBC1D7 at Ser-124. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 54-57 30143532-5 2018 Sequence analysis identified a putative site for both Akt-mediated phosphorylation and 14-3-3 binding at Ser-124, and we found that Akt phosphorylates TBC1D7 at Ser-124. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 132-135 30143532-5 2018 Sequence analysis identified a putative site for both Akt-mediated phosphorylation and 14-3-3 binding at Ser-124, and we found that Akt phosphorylates TBC1D7 at Ser-124. Serine 161-164 AKT serine/threonine kinase 1 Homo sapiens 54-57 30143532-5 2018 Sequence analysis identified a putative site for both Akt-mediated phosphorylation and 14-3-3 binding at Ser-124, and we found that Akt phosphorylates TBC1D7 at Ser-124. Serine 161-164 AKT serine/threonine kinase 1 Homo sapiens 132-135 30143532-10 2018 Our findings reveal that Akt activity determines the phosphorylation status of TBC1D7 at the phospho-switch Ser-124, which governs binding to either 14-3-3 or beta-TrCP2, resulting in increased or decreased stability of TBC1D7, respectively. Serine 108-111 AKT serine/threonine kinase 1 Homo sapiens 25-28 30047091-7 2018 Furthermore, we found that this compound inhibited phosphorylation of the protein kinase Akt at serine residue 473, but had no impact on phosphorylation at threonine 308. Serine 96-102 AKT serine/threonine kinase 1 Homo sapiens 89-92 30305866-9 2018 Furthermore, impairment of the antioxidant defense system caused by maternal serine deficiency was mediated by the Akt/AMPK/Sirt1 pathway. Serine 77-83 AKT serine/threonine kinase 1 Homo sapiens 115-118 30285764-9 2018 RESULTS: PDGF-BB markedly increased phosphorylation of mTORC2 substrate Akt, at serine 473 (S473) and threonine 450 (T450) in menin-/- MEFs but did not alter phosphorylation of mTORC1 substrates ribosomal protein S6 or eIF4B. Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 72-75 30249058-7 2018 The specific binding did not lead to the membrane translocation to phosphatidylinositol (3,4,5)-trisphosphate (PIP3), but directly activated the phosphorylation of AKT on Ser-473, induced the phosphorylation of the downstream molecules, glycogen synthase kinase 3beta (GSK3beta) and forkhead box O1 (FOXO1), and improved glucose metabolism. Serine 171-174 AKT serine/threonine kinase 1 Homo sapiens 164-167 29471001-8 2018 Furthermore, the active CXCL5-CXCR2 axis enhanced the phosphorylation of Akt at Ser 473 residue and that of glycogen synthase kinase-3 (GSK-3beta) at Ser 9 residue, and accelerated the nuclear accumulation of beta-catenin in PTC cells. Serine 80-83 AKT serine/threonine kinase 1 Homo sapiens 73-76 29883748-4 2018 Mechanistically, LPS treatment markedly upregulates TLR4 mediated inflammatory pathway which by activating mTORC2 induces Akt phosphorylation at serine 473 and that aggravate mTORC1 dependent scavenger receptors expression and consequent lipid accumulation in THP-1 macrophages. Serine 145-151 AKT serine/threonine kinase 1 Homo sapiens 122-125 30013652-10 2018 In addition, phosphorylation and subsequent activation of protein kinase B (Akt), which is known to phosphorylate GSK-3beta at serine 9 (Ser9), leading to its inhibition, were significantly decreased by juglone in LNCaP and LNCaP-AI cells. Serine 127-133 AKT serine/threonine kinase 1 Homo sapiens 76-79 28431901-6 2018 Clozapine downregulated LPS-induced Akt phosphorylation at Ser-473. Serine 59-62 AKT serine/threonine kinase 1 Homo sapiens 36-39 29707142-4 2018 In this study, we examined expression of AR and VDR, phosphorylation of AKT serine (Ser) 473 (AKT phospho-Ser473) and ERalpha Ser167 (ERalpha phospho-Ser167) by immunohistochemistry in ER-positive, HER2-negative early breast cancer. Serine 76-82 AKT serine/threonine kinase 1 Homo sapiens 72-75 29707142-4 2018 In this study, we examined expression of AR and VDR, phosphorylation of AKT serine (Ser) 473 (AKT phospho-Ser473) and ERalpha Ser167 (ERalpha phospho-Ser167) by immunohistochemistry in ER-positive, HER2-negative early breast cancer. Serine 84-87 AKT serine/threonine kinase 1 Homo sapiens 72-75 29377106-2 2018 Inhibitory Ser-9 phosphorylation of GSK3beta by AKT is an important mechanism for negative regulation of GSK3beta activity upon insulin stimulation. Serine 11-14 AKT serine/threonine kinase 1 Homo sapiens 48-51 29393871-5 2018 In addition, they stimulated the phosphorylation levels of the components of the insulin signaling pathway, including tyrosine phosphorylation of IRS-1, and serine phosphorylation of Akt and GSK-3beta. Serine 157-163 AKT serine/threonine kinase 1 Homo sapiens 183-186 29374705-10 2018 Combination of naftopidil and CQ reduced cell viability and phospho-AKT (Ser 473). Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 68-71 29377106-3 2018 Here, we report that Thr-7 and Thr-8 residues located in the AKT/PKB substrate consensus sequence on GSK3beta are essential for insulin-stimulated Ser-9 phosphorylation in vivo and for GSK3beta inactivation. Serine 147-150 AKT serine/threonine kinase 1 Homo sapiens 61-64 29242061-6 2018 These data suggest that a second Rac-binding site within the C-terminal region is opened by phosphorylation of Ser-525. Serine 111-114 AKT serine/threonine kinase 1 Homo sapiens 33-36 29077243-6 2018 In the ATL-cell lines and in the primary ATL samples, both dual inhibitors inhibited phosphorylation of AKT at serine-473, a target of mTORC2, as well as that of S6K, whereas the mTORC1 inhibitors only inhibited mTORC1. Serine 111-117 AKT serine/threonine kinase 1 Homo sapiens 104-107 29253569-8 2018 KGFR shRNA or Akt-mTORC1 inhibition not only blocked KGF-induced Nrf2 Ser-40 phosphorylation and activation, but also nullified KGF-mediated RPE cell protection against UV. Serine 70-73 AKT serine/threonine kinase 1 Homo sapiens 14-17 27975170-6 2018 Furthermore, antidepressants elevated the phosphorylation of extracellular signal-regulated-protein kinase (ERK1/2) and the serine/threonine kinase Akt. Serine 124-130 AKT serine/threonine kinase 1 Homo sapiens 148-151 29381395-8 2018 Western blot results of placental tissue showed a decrease in the expression levels of AKT-serine and threonine in the PE groups compared to the normotensives and a significantly higher expression in the GH groups compared to normotensives. Serine 91-97 AKT serine/threonine kinase 1 Homo sapiens 87-90 29212252-7 2017 Pharmacological inhibition and short hairpin RNA-mediated knockdown experiments showed that AKT-dependent mammalian target of rapamycin (mTOR) activation was involved in the phosphorylation of serine-727 of STAT3, which in turn stimulated the transcription of the CCR1 gene. Serine 193-199 AKT serine/threonine kinase 1 Homo sapiens 92-95 29114068-2 2017 The increased RhoA-GTP results from AKT phosphorylating three serines (S298, S329, and S567) in the DLC1 tumor suppressor, a Rho GTPase-activating protein (RhoGAP) associated with focal adhesions. Serine 62-69 AKT serine/threonine kinase 1 Homo sapiens 36-39 28976590-7 2017 Interestingly, following fMLP stimulation of TDM-activated neutrophils, AKT, a crucial kinase for neutrophil polarization and chemotaxis, showed prolonged phosphorylation at serine 473. Serine 174-180 AKT serine/threonine kinase 1 Homo sapiens 72-75 28912066-9 2017 Our results suggest that Epac and PKA activate eNOS via Ser 1177 phosphorylation by activating the PI3K/Akt pathway, and independently of AMPK or CaMKII activation or [Ca2+]c increase. Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 104-107 28669988-14 2017 This effect is accompanied by more pronounced insulin sensitivity and serine 473 phosphorylation of Akt in B/BAT of non-diabetic than in diabetic subjects. Serine 70-76 AKT serine/threonine kinase 1 Homo sapiens 100-103 28383821-14 2017 Endothelial nitric oxide synthase (eNOS) phosphorylation at serine 1177 and Akt phosphorylation at serine 308 and threonine 473 were significantly inhibited by NaHS. Serine 99-105 AKT serine/threonine kinase 1 Homo sapiens 76-79 28427860-7 2017 Cells with low levels of Akt Ser-473 phosphorylation were more resistant to the cytotoxic effects of mTOR inhibition with Torin-2, but not KU-0063794. Serine 29-32 AKT serine/threonine kinase 1 Homo sapiens 25-28 28427860-8 2017 Exposure to Torin-2 and KU-0063794 both potently and rapidly inhibited phosphorylation of Akt Ser-473 and Thr-308, and 4E-BP1 T37/46 in cell lines that included basal and nontype subtypes. Serine 94-97 AKT serine/threonine kinase 1 Homo sapiens 90-93 28427860-10 2017 Urothelial carcinomas characterized by high baseline Akt Ser-473 phosphorylation may be best suited for targeted mTOR therapies. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 53-56 28927088-7 2017 Restoration of PCDH8 decreased phosphorylated (p)-Rac-alpha serine/threonine-protein kinase (AKT) [Threonine (T)308/Serine (S)473] and p-glycogen synthase kinase-3beta (p-GSK3beta) (S9) protein expression, thereby reducing the level of beta-catenin when compared with the control. Serine 116-122 AKT serine/threonine kinase 1 Homo sapiens 50-59 28468838-4 2017 Tat appears to stabilise Mdm2 at the post-translational level by inducing its phosphorylation at serine-166 position through AKT. Serine 97-103 AKT serine/threonine kinase 1 Homo sapiens 125-128 28460125-5 2017 For the PI3K/AKT pathway, PKA activates the Ser/Thr PP1 complexed with the insulinlike growth factor 1 receptor (IGF-1R) and insulin receptor substrate 1 (IRS1) to dephosphorylate Ser residues on IRS1, authorizing phosphorylation of IRS1 by the IGF-1R to activate PI3K. Serine 44-47 AKT serine/threonine kinase 1 Homo sapiens 13-16 28460125-5 2017 For the PI3K/AKT pathway, PKA activates the Ser/Thr PP1 complexed with the insulinlike growth factor 1 receptor (IGF-1R) and insulin receptor substrate 1 (IRS1) to dephosphorylate Ser residues on IRS1, authorizing phosphorylation of IRS1 by the IGF-1R to activate PI3K. Serine 180-183 AKT serine/threonine kinase 1 Homo sapiens 13-16 28969041-5 2017 Aplysin treatment inhibited the phosphorylation levels of AKT (Ser-473) and AKT-dependent phosphorylation of FOXO3a (Ser-253) in breast cancer cell lines and breast cancer tissues. Serine 117-120 AKT serine/threonine kinase 1 Homo sapiens 76-79 28383821-16 2017 However, eNOS phosphorylation at serine 1177 and Akt phosphorylation at serine 308 and threonine 473 were significantly potentiated by AOAA. Serine 72-78 AKT serine/threonine kinase 1 Homo sapiens 49-52 28383821-17 2017 Cystathionine beta-synthase siRNA interference significantly increased eNOS phosphorylation at serine 1177 and Akt phosphorylation at serine 308 and threonine 473. Serine 134-140 AKT serine/threonine kinase 1 Homo sapiens 111-114 28279977-2 2017 We found that, after AKT-mediated phosphorylation at serine 319, FOXO1 binds to IQGAP1, a hub for activation of the MAPK pathway, and impedes IQGAP1-dependent phosphorylation of ERK1/2 (pERK1/2). Serine 53-59 AKT serine/threonine kinase 1 Homo sapiens 21-24 28443643-4 2017 Importantly, activated AKT directly phosphorylates FAF1 at Ser 582, which disrupts the FAF1-VCP complex and reduces FAF1 at the plasma membrane. Serine 59-62 AKT serine/threonine kinase 1 Homo sapiens 23-26 28322991-4 2017 The molecular basis showed that thyroxine receptor stimulation triggers Phosphatidyl Inositol 3-kinase (PI3K)/Akt signaling activation leading to the E3 ligase MDM2 phosphorylation at serine 166, which directly interacted with p53 for degradation. Serine 184-190 AKT serine/threonine kinase 1 Homo sapiens 110-113 28498467-4 2017 Decrease in the amount of AKT phosphorylated on serine (S) 473 indicated that PI3K/AKT signaling was suppressed in the cells. Serine 48-54 AKT serine/threonine kinase 1 Homo sapiens 26-29 28498467-4 2017 Decrease in the amount of AKT phosphorylated on serine (S) 473 indicated that PI3K/AKT signaling was suppressed in the cells. Serine 48-54 AKT serine/threonine kinase 1 Homo sapiens 83-86 27988337-8 2017 Significantly, Akt Ser-473 phosphorylation in PANC-1 cells was also inhibited by miR-101, but was augmented with antagomiR-101 expression. Serine 19-22 AKT serine/threonine kinase 1 Homo sapiens 15-18 27922982-13 2017 In addition, we confirmed that miR-21 could activate Akt pathway by phosphorylating Akt at Ser 473. Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 53-56 27922982-13 2017 In addition, we confirmed that miR-21 could activate Akt pathway by phosphorylating Akt at Ser 473. Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 84-87 27868170-5 2017 The phosphorylation of serine residues in the insulin receptor and IRS-1 molecule results in diminished enzymatic activity in the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Serine 23-29 AKT serine/threonine kinase 1 Homo sapiens 167-170 28123351-1 2017 Rictor is a key regulatory/structural subunit of the mammalian target of rapamycin complex 2 (mTORC2) and is required for phosphorylation of Akt at serine 473. Serine 148-154 AKT serine/threonine kinase 1 Homo sapiens 141-144 27965087-3 2017 In the present study, decreased SHIP and increased phospho-Akt (Ser 473, Thr 308) were found in renal tubular cells of diabetic mice accompanied by overexpression of connective tissue growth factor (CTGF) and extracellular matrix deposition versus normal mice. Serine 64-67 AKT serine/threonine kinase 1 Homo sapiens 59-62 27717743-4 2017 AKT1 has two distinct actions on GR transcriptional activity, one through segregation of GR in the cytoplasm by phosphorylating GR at Ser-134 and subsequent association of 14-3-3, and the other through direct modulation of GR transcriptional activity in the nucleus. Serine 134-137 AKT serine/threonine kinase 1 Homo sapiens 0-4 27393705-5 2017 RESULTS: A kinome-level screen and Kds analyses against a panel of 102 human kinase targets showed that Del binds to three lipid (PIK3CG, PIK3C2B, and PIK3CA) and six serine/threonine (PIM1, PIM3, mTOR, S6K1, PLK2, and AURKB) kinases, five of which belong to the PI3K/Akt/mTOR pathway. Serine 167-173 AKT serine/threonine kinase 1 Homo sapiens 268-271 27381982-2 2016 Here, we found that following B-cell receptor (BCR)-induced tyrosine phosphorylation of the cytoplasmic tyrosine kinase SYK and the adaptor BLNK, the AKT/PKB enzyme strongly induced BLNK (>100-fold) and SYK (>100-fold) serine/threonine phosphorylation (pS/pT). Serine 225-231 AKT serine/threonine kinase 1 Homo sapiens 150-153 27533087-5 2016 Moreover, we report that within such Ephrin B3/EphA2 complex both Akt Ser 129 and p38MAPK are found indicating a potential to drive migration/proliferation. Serine 70-73 AKT serine/threonine kinase 1 Homo sapiens 66-69 27856640-7 2016 Additional experiments indicate that this mechanism also occurs in breast cancer, thyroid, and preovulatory granulosa cells, suggesting that the PKA-dependent dephosphorylation of IRS1 Ser/Thr residues is a conserved mechanism by which GPCRs signal to activate the PI3K/AKT pathway downstream of the IGF1R. Serine 185-188 AKT serine/threonine kinase 1 Homo sapiens 270-273 27246912-1 2016 Akt substrate of 160 kDa (AS160) phosphorylation on Thr(642) and Ser(588) by Akt is essential for insulin"s full effect on glucose transport. Serine 65-68 AKT serine/threonine kinase 1 Homo sapiens 0-3 27381982-2 2016 Here, we found that following B-cell receptor (BCR)-induced tyrosine phosphorylation of the cytoplasmic tyrosine kinase SYK and the adaptor BLNK, the AKT/PKB enzyme strongly induced BLNK (>100-fold) and SYK (>100-fold) serine/threonine phosphorylation (pS/pT). Serine 225-231 AKT serine/threonine kinase 1 Homo sapiens 154-157 27323966-7 2016 We then test protein expression of p-GSK3beta(Ser-9), the downstream protein of PI3K/AKT, which was significantly decreased under the circumstance of GKN2 overexpression. Serine 46-49 AKT serine/threonine kinase 1 Homo sapiens 85-88 27020593-10 2016 Phosphorylations of Akt (Ser-473) and S6 were also decreased in WYE-354-treated HT-29 xenografts. Serine 25-28 AKT serine/threonine kinase 1 Homo sapiens 20-23 27602284-8 2016 The threonine-24 (Thr-24) and serine-319 (Ser-319) residues on Foxo1, and the Thr-32 residue on Foxo3a are phosphorylated by Akt, leading to cytoplasmic localization of Foxo. Serine 30-36 AKT serine/threonine kinase 1 Homo sapiens 125-128 27602284-8 2016 The threonine-24 (Thr-24) and serine-319 (Ser-319) residues on Foxo1, and the Thr-32 residue on Foxo3a are phosphorylated by Akt, leading to cytoplasmic localization of Foxo. Serine 42-45 AKT serine/threonine kinase 1 Homo sapiens 125-128 27300007-0 2016 Serine 707 of APPL1 is Critical for the Synaptic NMDA Receptor-Mediated Akt Phosphorylation Signaling Pathway. Serine 0-6 AKT serine/threonine kinase 1 Homo sapiens 72-75 27494022-9 2016 Exposure of H2O2 (1mM H2O2 for 30min) to DLA cells caused ROS accumulation and resulted in increased phosphorylation of PI3K and downstream proteins PDK1 and AKT (Ser-473 and Thr-308), cell survival factors BAD and ERK1/2, as well as TNFR1. Serine 163-166 AKT serine/threonine kinase 1 Homo sapiens 158-161 27493677-4 2016 The main signaling pathway regulating GSK3 activity through serine-phosphorylation is the phosphoinositide 3-kinase (PI3K)/phosphoinositide-dependent kinase-1 (PDK1)/Akt relay that catalyzes serine-phosphorylation and thus inactivation of GSK3. Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 166-169 27493677-4 2016 The main signaling pathway regulating GSK3 activity through serine-phosphorylation is the phosphoinositide 3-kinase (PI3K)/phosphoinositide-dependent kinase-1 (PDK1)/Akt relay that catalyzes serine-phosphorylation and thus inactivation of GSK3. Serine 191-197 AKT serine/threonine kinase 1 Homo sapiens 166-169 27481183-3 2016 At the protein expression level, compared to the lean, the phosphorylation of AMPK, and p-Akt at serine 473 were significantly reduced from the overweight (OW) and/or obese (OB); while the protein expression of p-JNK, cleaved caspase 3, CHOP and p-eIF2alpha were elevated from the OW and/or OB. Serine 97-103 AKT serine/threonine kinase 1 Homo sapiens 90-93 26959555-2 2016 However, it is recognized that the activation of Akt1 requires phosphorylation events at threonine (T) 308 and serine (S) 473. Serine 111-117 AKT serine/threonine kinase 1 Homo sapiens 49-53 27207509-5 2016 In contrast, insulin, but not LPS, induced mammalian target of rapamycin complex 2 (mTORC2)-dependent phosphorylation of Akt on Ser(473) and FoxO1/O3a on Thr(24/32) in leukocytes and neutrophils. Serine 128-131 AKT serine/threonine kinase 1 Homo sapiens 121-124 27300007-6 2016 Furthermore, we identified serine 707 of APPL1, a predicted phosphorylation site within the PDZ-binding motif at the C-terminus, as critical for the binding of APPL1 to PSD95, as well as for activation of the Akt signaling pathway during synaptic activity. Serine 27-33 AKT serine/threonine kinase 1 Homo sapiens 209-212 27300007-7 2016 This suggests that serine 707 of APPL1 is a potential phosphorylation site and may be involved in regulating the neuroprotective Akt signaling pathway that depends on synaptic NMDAR activity. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 129-132 27154977-7 2016 Additionally, we have found that SNG inhibits Akt, a key anti-apoptotic protein kinase by dephosphorylating it at Ser(473), leading to the dephosphorylation of its downstream targets, GSK3beta and mTOR. Serine 114-117 AKT serine/threonine kinase 1 Homo sapiens 46-49 27463705-9 2016 These results indicate that fast non-genomic effects of ER with downstream signaling through the PI3K/Akt pathway and consecutive eNOS phosphorylation at serine 1177 are involved in BA-induced eNOS activation. Serine 154-160 AKT serine/threonine kinase 1 Homo sapiens 102-105 27412013-4 2016 Inhibiting or silencing DNA-PKcs suppressed AKT Ser-473 phosphorylation and HIF-2alpha expression. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 44-47 27099352-4 2016 We found that inhibitions of calpain-2 by using calpain inhibitor MDL28170 and calpain-2 small interfering RNA attenuated Akt phosphorylations at serine-473 (S473) and threonine-308 (T308), as well as collagen synthesis and cell proliferation of PASMCs induced by PDGF. Serine 146-152 AKT serine/threonine kinase 1 Homo sapiens 122-125 27340866-3 2016 Herein, we report that AKT directly interacts with PKM2 and phosphorylates it at Ser-202, which is essential for the nuclear translocation of PKM2 protein under stimulation of IGF-1. Serine 81-84 AKT serine/threonine kinase 1 Homo sapiens 23-26 27187380-5 2016 The levels of phosphorylated-HSP27 (Ser-78) were correlated with the levels of phosphorylated-p38 MAP kinase and phosphorylated-Akt in the platelets stimulated by 10 microM TRAP but not with those of phosphorylated-p44/p42 MAP kinase. Serine 36-39 AKT serine/threonine kinase 1 Homo sapiens 128-131 26994872-5 2016 Most importantly, TA markedly reversed the inhibition of protein expression of phosphorylated Akt (Ser 473) and phosphorylated GSK3beta (Ser 9) caused by MPP(+). Serine 99-102 AKT serine/threonine kinase 1 Homo sapiens 94-97 27273097-7 2016 Through bioinformatics and cell-based experiments, we identified the AKT-repressed signal as glycogen synthase kinase 3 (GSK3)-catalyzed phosphorylation of Ser(37) on the long form of the transcription factor GATA6. Serine 156-159 AKT serine/threonine kinase 1 Homo sapiens 69-72 27067055-7 2016 ACLY becomes phosphorylated on serine 455 in T lymphocytes upon IL-2-driven activation of AKT, and depletion or inactivation of ACLY compromises IL-2-promoted T-cell growth. Serine 31-37 AKT serine/threonine kinase 1 Homo sapiens 90-93 27121065-4 2016 We also found that the phosphorylation of Akt at Ser 473 might be mediated through p-ATM (Ser 1981). Serine 49-52 AKT serine/threonine kinase 1 Homo sapiens 42-45 27121065-4 2016 We also found that the phosphorylation of Akt at Ser 473 might be mediated through p-ATM (Ser 1981). Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 42-45 26869446-8 2016 In HeLa cells transfected with Cx43 (HeLa-Cx43), LA induced phosphorylation of surface Cx43 at serine 373 (S373), site for Akt phosphorylation. Serine 95-101 AKT serine/threonine kinase 1 Homo sapiens 123-126 26945062-2 2016 Akt1-3 kinases are specifically activated by two phosphorylation events on residues Thr(308) and Ser(473) upon growth factor signaling, which subsequently phosphorylate a vast cohort of downstream targets. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 0-4 26945062-5 2016 First, we show that the accumulation of multiple phosphorylation events on Akt forms occur concurrently with Ser(473) and Thr(308) phosphorylation upon acute PI3K activation and provide evidence for uncoupling of Ser(473) and Thr(308) phosphorylation, as well as differential sensitivities of Akt1 forms upon PI3K inhibition. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 75-78 26945062-5 2016 First, we show that the accumulation of multiple phosphorylation events on Akt forms occur concurrently with Ser(473) and Thr(308) phosphorylation upon acute PI3K activation and provide evidence for uncoupling of Ser(473) and Thr(308) phosphorylation, as well as differential sensitivities of Akt1 forms upon PI3K inhibition. Serine 213-216 AKT serine/threonine kinase 1 Homo sapiens 75-78 26945062-7 2016 Third, we show differential sensitivities of Ser(473)-Akt species to PTEN deletion in mature neurons, which suggests inherent differences in the Akt pools that are accessible to growth factors as compared with the pools that are controlled by PTEN. Serine 45-48 AKT serine/threonine kinase 1 Homo sapiens 54-57 26869446-10 2016 Thus, the increase in membrane permeability induced by LA is mediated by an intracellular signaling pathway activated by GPR40 that leads to an increase in membrane levels of Cx43 phosphorylated at serine 373 via Akt. Serine 198-204 AKT serine/threonine kinase 1 Homo sapiens 213-216 27105349-4 2016 Using synthetic peptides and a set of recombinant proteins expressing mutants of the LBR RS domain we now demonstrate that while all serines of the RS domain represent more or less equal phosphoacceptor sites for SRPK1, Ser80 and Ser82 are mainly targeted by Akt. Serine 133-140 AKT serine/threonine kinase 1 Homo sapiens 259-262 26912789-2 2016 We show that PRMT5 interacts with and methylates ASK1 at arginine residue 89 and thereby negatively regulates its activity by promoting the interaction between ASK1 and Akt and thus phosphorylating ASK1 at serine residue 83. Serine 206-212 AKT serine/threonine kinase 1 Homo sapiens 169-172 27027448-1 2016 The ser-thr Akt plays a critical role in the regulation of cell survival, cell growth and proliferation, as well as energy metabolism and is dysregulated in many cancers. Serine 4-7 AKT serine/threonine kinase 1 Homo sapiens 12-15 26729266-10 2016 In a human PASMC line, immunoblot analysis showed the following: (1) eNOS expression, (2) Ser(1177) phosphorylation by U46619 and H2O2, and (3) Akt activation (Ser(473) phosphorylation) by U46619. Serine 160-163 AKT serine/threonine kinase 1 Homo sapiens 144-147 27076174-3 2016 In primary T cells treated with exogenous Nef or acutely infected with Nef-expressing HIV-1 in vitro, Akt became phosphorylated on serine(473) and threonine(308). Serine 131-137 AKT serine/threonine kinase 1 Homo sapiens 102-105 26983022-4 2016 RACalpha serine/threonine-protein kinase (AKT1) was a highly essential central protein. Serine 9-15 AKT serine/threonine kinase 1 Homo sapiens 42-46 27294206-7 2016 BEZ235 affects PI3K/Akt/mTOR signaling pathway by increasing the phosphorylation of AKT at Ser(473) and RAS/RAF/MEK/ERK pathway by decreasing the phosphorylation of ERK at Tyr(204). Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 20-23 27294206-7 2016 BEZ235 affects PI3K/Akt/mTOR signaling pathway by increasing the phosphorylation of AKT at Ser(473) and RAS/RAF/MEK/ERK pathway by decreasing the phosphorylation of ERK at Tyr(204). Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 84-87 26393424-0 2015 CCN1 acutely increases nitric oxide production via integrin alphavbeta3-Akt-S6K-phosphorylation of endothelial nitric oxide synthase at the serine 1177 signaling axis. Serine 140-146 AKT serine/threonine kinase 1 Homo sapiens 72-75 26868304-7 2016 We found that protein phosphorylation of Akt and GSK3beta varied over the 24h and 48 h time points, with constitutive phosphorylation of Akt at serine 473 (pAkt) decreasing to its most significant level when treated with Wnt3a+sFRP4 at the 24h time point. Serine 144-150 AKT serine/threonine kinase 1 Homo sapiens 41-44 26868304-7 2016 We found that protein phosphorylation of Akt and GSK3beta varied over the 24h and 48 h time points, with constitutive phosphorylation of Akt at serine 473 (pAkt) decreasing to its most significant level when treated with Wnt3a+sFRP4 at the 24h time point. Serine 144-150 AKT serine/threonine kinase 1 Homo sapiens 137-140 26792718-7 2016 At the molecular level, WYE-687 concurrently inhibited activation of mTORC1 (p70S6K1 and S6 phosphorylations) and mTORC2 (AKT Ser-473 and FoxO1/3a phosphorylations), whiling downregulating mTORC1/2-regulated genes (Bcl-xL and hypoxia-inducible factor 1/2alpha) in both HL-60/U937 cells and human AML progenitor cells. Serine 126-129 AKT serine/threonine kinase 1 Homo sapiens 122-125 26781309-17 2016 Western blot analysis revealed dephosphorylation of Akt (Ser 473) with activation of p53, Bax, Bak, Bid and reduction in Bcl-2 and Bcl-xL levels. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 52-55 26311050-7 2016 PEBP4 interacted with AKT and promoted the phosphorylation of serine 473 in AKT. Serine 62-68 AKT serine/threonine kinase 1 Homo sapiens 76-79 26839558-11 2016 The reduction of Akt-p at Ser-473 by NTG, DETA NONOate, and 8-Br-cGMP was significantly inhibited by ODQ, PKG-I. Serine 26-29 AKT serine/threonine kinase 1 Homo sapiens 17-20 26483070-5 2016 We measured insulin sensitivity as the homeostasis model assessment-estimated insulin resistance (HOMA-IR) and by the euglycemic hyperinsulinemic clamp, and phosphorylation of protein kinase B (Akt) on Ser473 and Thr308 and of GSK3 alpha-beta on Ser 9 and Ser21 in skeletal muscle biopsy specimens by Western blot analysis. Serine 202-205 AKT serine/threonine kinase 1 Homo sapiens 194-197 26483070-13 2016 Although other mechanisms of action, such as the effect of weight loss and reduced food intake, cannot be excluded, the reduction of muscle Akt hyperphosphorylation on the serine residue can play a role in the early improvement of insulin sensitivity. Serine 172-178 AKT serine/threonine kinase 1 Homo sapiens 140-143 26393424-7 2015 Consistently, CCN1 increased the phosphorylation of Akt-Ser(473) and S6K-Thr(389). Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 52-55 26393424-8 2015 However, CCN1 did not alter the expression or secretion of VEGF, a known downstream factor of CCN1 and a potential upstream factor of Akt-mediated eNOS-Ser(1177) phosphorylation. Serine 152-155 AKT serine/threonine kinase 1 Homo sapiens 134-137 26393424-12 2015 In conclusion, CCN1 acutely increases NO production via activation of a signaling axis in integrin alphavbeta3-Akt-S6K-eNOS-Ser(1177) phosphorylation, suggesting an important role for CCN1 in vasodilation. Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 111-114 25633180-9 2015 Moreover, 17-AAG pretreatment decreased the level of phsophorylated Akt at serine 473 residue and suppressed active Akt-dependent inactivation of glycogen synthase kinase 3beta. Serine 75-81 AKT serine/threonine kinase 1 Homo sapiens 68-71 26025205-5 2015 Threonine 308 and serine 473 are the two critical phosphorylation sites of Akt1. Serine 18-24 AKT serine/threonine kinase 1 Homo sapiens 75-79 26527864-9 2015 The phosphorylation of insulin receptor substrate-1 (IRS-1) Ser(307) and Akt Ser(473) was increased, whereas the protein expression of IRS-1, Akt, and glucose transporter-4 was decreased. Serine 77-80 AKT serine/threonine kinase 1 Homo sapiens 73-76 26149501-5 2015 We found that Akt phosphorylated PIP5Kgamma90 specifically at serine 555 (S555) in vitro and in epidermal growth factor (EGF)-treated cells phosphoinositide 3-kinase-dependently. Serine 62-68 AKT serine/threonine kinase 1 Homo sapiens 14-17 26387611-6 2015 In addition, Akt(Ser(473)) and p53(Ser(15)/Ser(315)) dephosphorylation occurred. Serine 17-20 AKT serine/threonine kinase 1 Homo sapiens 13-16 26374334-1 2015 UNLABELLED: The serine/threonine protein kinase Akt plays a critical role in regulating proliferation, growth, and survival through phosphorylation of different downstream substrates. Serine 16-22 AKT serine/threonine kinase 1 Homo sapiens 48-51 26458825-3 2015 During etoposide-induced apoptosis of HeLa cells, Akt enhances the interaction of second mitochondria-derived activator of caspases/direct IAP binding protein with low pI (Smac/DIABLO) and X-linked inhibitor of apoptosis protein by phosphorylating Smac at serine 67, and thus promotes apoptosis. Serine 256-262 AKT serine/threonine kinase 1 Homo sapiens 50-53 26201515-4 2015 Modifications of amino acids surrounding the Akt1 C-terminal mTORC2 (mammalian target of rapamycin complex 2) phosphorylation site (Ser(473)) increased phosphatase resistance of the phosphorylated activation loop (pThr(308)) and amplified Akt phosphorylation. Serine 132-135 AKT serine/threonine kinase 1 Homo sapiens 45-49 26201515-4 2015 Modifications of amino acids surrounding the Akt1 C-terminal mTORC2 (mammalian target of rapamycin complex 2) phosphorylation site (Ser(473)) increased phosphatase resistance of the phosphorylated activation loop (pThr(308)) and amplified Akt phosphorylation. Serine 132-135 AKT serine/threonine kinase 1 Homo sapiens 45-48 26267517-7 2015 Our study demonstrates that there is frequent and unexpected cross-talk that occurs between tyrosine signaling pathways and serine/threonine signaling pathways activated by the canonical PI3K-AKT axis. Serine 124-130 AKT serine/threonine kinase 1 Homo sapiens 192-195 25893736-3 2015 Mahanine suppression of rictor gene expression and consequent attenuation of its protein expression affected the inhibition of mTOR (Ser-2481) and Akt (Ser-473) phosphorylation. Serine 152-155 AKT serine/threonine kinase 1 Homo sapiens 147-150 26170450-0 2015 Akt Phosphorylates Wnt Coactivator and Chromatin Effector Pygo2 at Serine 48 to Antagonize Its Ubiquitin/Proteasome-mediated Degradation. Serine 67-73 AKT serine/threonine kinase 1 Homo sapiens 0-3 26170450-5 2015 Furthermore, Pygo2 is phosphorylated at multiple residues, and Akt-mediated phosphorylation at serine 48 leads to its decreased ubiquitylation and increased stability. Serine 95-101 AKT serine/threonine kinase 1 Homo sapiens 63-66 26031292-5 2015 Within the PI3K/Akt pathway, we found activation of Akt (serine 473) to be significantly increased at 5 hours while mTOR (serine 2448) was strongly activated early and was sustained over 24 hours after SSL. Serine 57-63 AKT serine/threonine kinase 1 Homo sapiens 16-19 26031292-5 2015 Within the PI3K/Akt pathway, we found activation of Akt (serine 473) to be significantly increased at 5 hours while mTOR (serine 2448) was strongly activated early and was sustained over 24 hours after SSL. Serine 57-63 AKT serine/threonine kinase 1 Homo sapiens 52-55 26031292-5 2015 Within the PI3K/Akt pathway, we found activation of Akt (serine 473) to be significantly increased at 5 hours while mTOR (serine 2448) was strongly activated early and was sustained over 24 hours after SSL. Serine 122-128 AKT serine/threonine kinase 1 Homo sapiens 16-19 25979648-6 2015 The anticancer effect of delta-T3 was achieved by its up-regulation of cyclin-dependent kinase inhibitors (p21 and p27), the activation of caspases and the suppression of phosphorylation of protein kinase B (Akt) at Thr(308) and Ser(473). Serine 229-232 AKT serine/threonine kinase 1 Homo sapiens 208-211 26055712-5 2015 A large scale proteomics screen revealed that connecdenn 1 is phosphorylated at residues Ser-536 and Ser-538 in an Akt-dependent manner in response to insulin stimulation of adipocytes. Serine 89-92 AKT serine/threonine kinase 1 Homo sapiens 115-118 26055712-5 2015 A large scale proteomics screen revealed that connecdenn 1 is phosphorylated at residues Ser-536 and Ser-538 in an Akt-dependent manner in response to insulin stimulation of adipocytes. Serine 101-104 AKT serine/threonine kinase 1 Homo sapiens 115-118 26158630-3 2015 It has been reported that ligand-independent phosphorylation of EphA2 at Ser-897 is induced by Akt. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 95-98 25979114-5 2015 Emerging evidence suggests that two key post-translational modifications of polyglutamine-expanded androgen receptor, namely serine phosphorylation by protein kinase B/Akt and arginine methylation by protein arginine methyltransferases, occur at the same consensus site, are mutually exclusive, and have opposing effects on neurotoxicity. Serine 125-131 AKT serine/threonine kinase 1 Homo sapiens 168-171 26152725-6 2015 In addition, the noted inhibitory effect of HER2 on MLK3 kinase activity was mediated via its phosphorylation on Ser(674) by AKT and that pharmacological inhibitors of PI3K/AKT prevented trastuzumab- and lapatinib-induced stimulation of MLK3 activity. Serine 113-116 AKT serine/threonine kinase 1 Homo sapiens 125-128 26152725-6 2015 In addition, the noted inhibitory effect of HER2 on MLK3 kinase activity was mediated via its phosphorylation on Ser(674) by AKT and that pharmacological inhibitors of PI3K/AKT prevented trastuzumab- and lapatinib-induced stimulation of MLK3 activity. Serine 113-116 AKT serine/threonine kinase 1 Homo sapiens 173-176 25797048-5 2015 DiC8-PIP3 induced the phosphorylation of Akt-Ser(473) which was reduced by the Akt inhibitor IV, wortmannin and EGTA (suggesting a dependence on Ca(2+) entry). Serine 45-48 AKT serine/threonine kinase 1 Homo sapiens 41-44 25797048-5 2015 DiC8-PIP3 induced the phosphorylation of Akt-Ser(473) which was reduced by the Akt inhibitor IV, wortmannin and EGTA (suggesting a dependence on Ca(2+) entry). Serine 45-48 AKT serine/threonine kinase 1 Homo sapiens 79-82 25944616-4 2015 Additional biochemical and biological studies revealed that serine 2152 (S2152) of filamin A is phosphorylated by activated Akt in the cells treated with As(3+). Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 124-127 25678401-8 2015 Mechanistically, ACK1 phosphorylated AKT at Thr 308 and Ser 473 and activated the AKT pathway to up-regulate the transcription factor POU2F1, which directly bound to the promoter region of its novel target gene ECD and thus regulated ECD expression in GC cells. Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 37-40 25882230-8 2015 It also suppressed NF-kappaB translocated to the nucleus and inhibited cAMP response element-binding protein (CREB) Ser-133 phosphorylation level in the phosphoinositide 3-kinase/protein kinase B/CREB (PI3K/AKT/CREB) pathway. Serine 116-119 AKT serine/threonine kinase 1 Homo sapiens 207-210 25935150-5 2015 This effect resulted in blockade of agonist-mediated phosphorylation of Akt and eNOS at serine 1179. Serine 88-94 AKT serine/threonine kinase 1 Homo sapiens 72-75 25560489-2 2015 The PAKs are highly conserved serine/threonine and effector proteins for Cdc42 and Rac and are essential in regulating cell motility and survival. Serine 30-36 AKT serine/threonine kinase 1 Homo sapiens 83-86 25831148-3 2015 The anti-miRNAs significantly reduced miR-21 and miR-95 levels, increased PTEN, SNX1, and SGPP1 protein expression, but reduced Akt Ser(473) and Thr(308) phosphorylation. Serine 132-135 AKT serine/threonine kinase 1 Homo sapiens 128-131 26191127-10 2015 Furthermore, we observed mitochondrial transmembrane depolarization along with decreased phosphorylation levels for PI3K, AKT (Ser 473), AKT (Thr 308), GSK-3beta, and BAD. Serine 127-130 AKT serine/threonine kinase 1 Homo sapiens 122-125 25784556-8 2015 A direct correlation between Ser(P)(473)-AKT and Ser(P)(401)-GATA2 was evident, and inhibition of AKT phosphorylation by the PI3K inhibitor LY294002 blocked Ser(401)-GATA2 phosphorylation and the increase in GRB10 expression. Serine 49-52 AKT serine/threonine kinase 1 Homo sapiens 41-44 25667991-7 2015 RESULTS: Our data showed that Abeta1-42 oligomers inhibited insulin-induced serine phosphorylation of Akt at 473 and GSK3beta at serine 9, as well as glycogen storage. Serine 76-82 AKT serine/threonine kinase 1 Homo sapiens 102-105 25784556-8 2015 A direct correlation between Ser(P)(473)-AKT and Ser(P)(401)-GATA2 was evident, and inhibition of AKT phosphorylation by the PI3K inhibitor LY294002 blocked Ser(401)-GATA2 phosphorylation and the increase in GRB10 expression. Serine 49-52 AKT serine/threonine kinase 1 Homo sapiens 41-44 25784556-3 2015 To identify the additional mechanism responsible for the decrease in Ser(P)(473)-AKT, studies were performed in human HepG2 cells and in C57BL/6J mice. Serine 69-72 AKT serine/threonine kinase 1 Homo sapiens 81-84 25784556-10 2015 These mechanisms converge to inhibit the insulin-induced increase in Ser(P)(473)-AKT. Serine 69-72 AKT serine/threonine kinase 1 Homo sapiens 81-84 25784556-6 2015 The combination of GRB10 silencing with BCL10 silencing and the reactive oxygen species inhibitor Tempol completely reversed the decline in Ser(P)(473)-AKT. Serine 140-143 AKT serine/threonine kinase 1 Homo sapiens 152-155 25784556-8 2015 A direct correlation between Ser(P)(473)-AKT and Ser(P)(401)-GATA2 was evident, and inhibition of AKT phosphorylation by the PI3K inhibitor LY294002 blocked Ser(401)-GATA2 phosphorylation and the increase in GRB10 expression. Serine 29-32 AKT serine/threonine kinase 1 Homo sapiens 41-44 25784556-11 2015 They provide internal feedback, mediated by Ser(P)(473)-AKT, Ser(P)(401)-GATA2, and nuclear GATA2, which links the opposing effects of serine and tyrosine phosphorylations of IRS1 and can modulate insulin responsiveness. Serine 44-47 AKT serine/threonine kinase 1 Homo sapiens 56-59 25784556-11 2015 They provide internal feedback, mediated by Ser(P)(473)-AKT, Ser(P)(401)-GATA2, and nuclear GATA2, which links the opposing effects of serine and tyrosine phosphorylations of IRS1 and can modulate insulin responsiveness. Serine 135-141 AKT serine/threonine kinase 1 Homo sapiens 56-59 25633838-5 2015 The expression of VEGF-A and phosphorylated Ser(473)-AKT, cell proliferation, migration and metastasis were enhanced in EZH2-overexpressing A549 cells, but inhibited in parental H2087 cells with EZH2 silencing or GSK126 treatment. Serine 44-47 AKT serine/threonine kinase 1 Homo sapiens 53-56 25501279-6 2015 Mechanistically, PCAF-mediated Akt1 acetylation enhanced Akt1 phosphorylation at both sites of Thr(308) and Ser(473) and further promoted the proliferation of glioblastoma cells. Serine 108-111 AKT serine/threonine kinase 1 Homo sapiens 31-35 25609086-3 2015 In an effort to identify novel substrates of B56-containing PP2As, we found that B56-containing PP2As and Akt act antagonistically to control reversible phosphorylation of Fam13a on Ser-322. Serine 182-185 AKT serine/threonine kinase 1 Homo sapiens 106-109 25609086-6 2015 When Ser-322 is phosphorylated by Akt, the binding between Fam13a and 14-3-3 is enhanced, leading to cytoplasmic sequestration of Fam13a. Serine 5-8 AKT serine/threonine kinase 1 Homo sapiens 34-37 25575882-7 2015 Without affecting PI3K-IB, ouabain doubled PI3K-IA activity and Akt phosphorylation at Ser(473). Serine 87-90 AKT serine/threonine kinase 1 Homo sapiens 64-67 25673820-11 2015 Doses of PI3K/TOR-KIs that enhanced MEK/ERK activation coincided with those that inhibited mTORC2-mediated AKT phosphorylation on Ser(473), suggesting a role of mTORC2. Serine 130-133 AKT serine/threonine kinase 1 Homo sapiens 107-110 25902785-7 2015 Phosphorylation of Akt (Ser(473)) decreased at 6 h in both trials (P < 0.05). Serine 24-27 AKT serine/threonine kinase 1 Homo sapiens 19-22 25761241-7 2015 Knockdown of Tspan8 by the targeted siRNAs prevented mTOR-rictor (mTORC2) assembly as well as phosphorylation of AKT (Ser-473) and protein kinase C alpha (PKCalpha) in U251MG cells. Serine 118-121 AKT serine/threonine kinase 1 Homo sapiens 113-116 25556058-4 2015 Conversely, activation-specific, Ser/Thr phosphorylation of MEK and Akt proteins was stable in Man-A-resistant tumors suggesting that stimulation of Ser/Thr PPase activity might be required for Man-A tumoricidal activity. Serine 33-36 AKT serine/threonine kinase 1 Homo sapiens 68-71 25501279-6 2015 Mechanistically, PCAF-mediated Akt1 acetylation enhanced Akt1 phosphorylation at both sites of Thr(308) and Ser(473) and further promoted the proliferation of glioblastoma cells. Serine 108-111 AKT serine/threonine kinase 1 Homo sapiens 57-61 25564612-3 2015 Here, we found that live P. gingivalis, but not heat-killed P. gingivalis, reduced Akt phosphorylation at both Thr-308 and Ser-473, which implies a decrease in Akt activity. Serine 123-126 AKT serine/threonine kinase 1 Homo sapiens 83-86 25686839-6 2015 The AKT pathway is known to phosphorylate XIAP at serine 87 leading to protein stability and PN1 expression is shown to interfere with this process. Serine 50-56 AKT serine/threonine kinase 1 Homo sapiens 4-7 25428717-12 2015 In dependence of the stimulus we found a temporal difference for the characteristic phosphorylation time of 20 and 150 seconds for the Ser-473 and Thr-308 residues on the Akt kinase, respectively. Serine 135-138 AKT serine/threonine kinase 1 Homo sapiens 171-174 24038446-4 2015 Our data reveals that Akt could bind to and phosphorylate Smac at serine residue 67, which enhances the ability of Smac to interact with the cytosolic X-chromosome linked IAP (XIAP) protein. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 22-25 25309720-3 2014 The PI3K-dependent Akt serine/threonine phosphorylation by PDK1 and mTORC2 has long been thought to be the primary mechanism accounting for Akt activation. Serine 23-29 AKT serine/threonine kinase 1 Homo sapiens 19-22 25569096-5 2015 ATCM activated the phosphorylation of ERK1/2, JNK and Akt at serine 308 in SH-SY5Y cells. Serine 61-67 AKT serine/threonine kinase 1 Homo sapiens 54-57 25370471-7 2015 Levels of phosphorylated serine-473 AKT were suppressed >90% in PRP at 3 hours after dose at the MTD and in tumor at pictilisib doses associated with AUC >20 h mumol/L. Serine 25-31 AKT serine/threonine kinase 1 Homo sapiens 36-39 25239188-2 2014 EphA2, which is overexpressed in a variety of human cancers, is phosphorylated by Akt on serine 897 and mediates ligand ephrin-independent promotion of anoikis resistance through the RhoG activator Ephexin4. Serine 89-95 AKT serine/threonine kinase 1 Homo sapiens 82-85 24977346-6 2014 Incubation with DETA NONOate (a stable NO donor) and 8-Br-cGMP (a cell membrane permeable analog of cGMP) attenuated Akt phosphorylation at Ser-473, which was prevented by Rp-8-Br-PET-cGMPS (a specific inhibitor of PKG) and calyculin A (an inhibitor of protein phosphatase 1 and 2A) but not by okadaic acid (a selective inhibitor of protein phosphatase 2A). Serine 140-143 AKT serine/threonine kinase 1 Homo sapiens 117-120 25590801-0 2015 Phosphorylation of the translation initiation factor eIF2alpha at serine 51 determines the cell fate decisions of Akt in response to oxidative stress. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 114-117 25790097-8 2015 These findings suggest a role of IGFIR/Akt axis in regulating glycolysis by Ser/Thr PKM2 phosphorylation in cancer cells. Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 39-42 25253241-8 2014 Furthermore, both Akt isoforms phosphorylated Dab2 at Ser residues 448 and 449. Serine 54-57 AKT serine/threonine kinase 1 Homo sapiens 18-21 25674239-4 2014 The mTORC2 pathway involves downstream activation of nuclear factor (NF)-kappaB and p-Akt (Ser 473). Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 86-89 25309720-3 2014 The PI3K-dependent Akt serine/threonine phosphorylation by PDK1 and mTORC2 has long been thought to be the primary mechanism accounting for Akt activation. Serine 23-29 AKT serine/threonine kinase 1 Homo sapiens 140-143 25074979-3 2014 In various breast cancer cell lines, we found that SIRT6 was phosphorylated at Ser(338) by the kinase AKT1, which induced the interaction and ubiquitination of SIRT6 by MDM2, targeting SIRT6 for protease-dependent degradation. Serine 79-82 AKT serine/threonine kinase 1 Homo sapiens 102-106 25122066-6 2014 Combination of simvastatin and metformin decreased Akt Ser-473 and Thr-308 phosphorylation and AMPKalpha Ser-485/491 phosphorylation; increased Thr-172 phosphorylation and AMPKalpha activity, as assessed by increased Ser-79 and Ser-872 phosphorylation of acetyl-CoA carboxylase and HMG-CoAR, respectively; decreased HMG-CoAR activity; and reduced total cellular cholesterol and its synthesis in both cell lines. Serine 55-58 AKT serine/threonine kinase 1 Homo sapiens 51-54 24990947-6 2014 Importantly, IKKalpha is shown to phosphorylate mTOR at serine 1415 in a manner dependent on Akt to promote mTORC1 activity. Serine 56-62 AKT serine/threonine kinase 1 Homo sapiens 93-96 24928510-6 2014 Gad8 is phosphorylated by TORC2 at Ser-546 (equivalent to AKT Ser-473), leading to its activation. Serine 35-38 AKT serine/threonine kinase 1 Homo sapiens 58-61 24928510-6 2014 Gad8 is phosphorylated by TORC2 at Ser-546 (equivalent to AKT Ser-473), leading to its activation. Serine 62-65 AKT serine/threonine kinase 1 Homo sapiens 58-61 25179402-4 2014 In addition, it stimulated the phosphorylation of ERK1/2 at Thr 202/Tyr 204 and of Akt1 at Ser 473 and the nuclear translocation of EGFR, STAT3, c-Jun, and c-Fos. Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 83-87 25086031-11 2014 These findings support a model in which PKC-mediated phosphorylation of CEP4 at Ser(18) and Ser(80) causes its dissociation from Cdc42, thereby increasing its affinity for TEM4 and producing Rac activation, filopodium formation, and cell motility. Serine 80-83 AKT serine/threonine kinase 1 Homo sapiens 191-194 24676635-7 2014 This change was associated with higher renal cortical phosphorylation of AKT at serine 473 (Ser(473)), which is the AKT site phosphorylated by mammalian target of rapamycin complex-2 (mTORC2). Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 73-76 24676635-7 2014 This change was associated with higher renal cortical phosphorylation of AKT at serine 473 (Ser(473)), which is the AKT site phosphorylated by mammalian target of rapamycin complex-2 (mTORC2). Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 116-119 24676635-7 2014 This change was associated with higher renal cortical phosphorylation of AKT at serine 473 (Ser(473)), which is the AKT site phosphorylated by mammalian target of rapamycin complex-2 (mTORC2). Serine 92-95 AKT serine/threonine kinase 1 Homo sapiens 73-76 24676635-7 2014 This change was associated with higher renal cortical phosphorylation of AKT at serine 473 (Ser(473)), which is the AKT site phosphorylated by mammalian target of rapamycin complex-2 (mTORC2). Serine 92-95 AKT serine/threonine kinase 1 Homo sapiens 116-119 24621265-6 2014 Moreover, COX-2-derived PGE2 signals the activation of the PI3-K/AKT and PKA/CREB pathways via cyclic AMP; these pathways transactivate the NF-kappaB p65 subunit via phosphorylation at Ser 536 and Ser 276, leading to IL-1beta synthesis. Serine 185-188 AKT serine/threonine kinase 1 Homo sapiens 65-68 24621265-6 2014 Moreover, COX-2-derived PGE2 signals the activation of the PI3-K/AKT and PKA/CREB pathways via cyclic AMP; these pathways transactivate the NF-kappaB p65 subunit via phosphorylation at Ser 536 and Ser 276, leading to IL-1beta synthesis. Serine 197-200 AKT serine/threonine kinase 1 Homo sapiens 65-68 24743054-8 2014 Rather, ET-3 stimulation of melanocytes promotes Akt phosphorylation at Ser 473, which is suppressed by PI3K inhibition or silencing of Akt, in turn blocking ET-3 upregulation of MCAM. Serine 72-75 AKT serine/threonine kinase 1 Homo sapiens 49-52 24743054-8 2014 Rather, ET-3 stimulation of melanocytes promotes Akt phosphorylation at Ser 473, which is suppressed by PI3K inhibition or silencing of Akt, in turn blocking ET-3 upregulation of MCAM. Serine 72-75 AKT serine/threonine kinase 1 Homo sapiens 136-139 24654606-7 2014 KEY RESULTS: Morphine stimulated Akt phosphorylation on Ser(473) and Thr(308) in a dose- and time-dependent manner indicating a functional mu-opioid receptor/Akt signalling pathway in mu-SK-N-LO cells. Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 33-36 25050889-8 2014 In addition, AKT serine phosphorylation was higher in PT-SGA compared to PT-AGA and T-SGA placentas (90% and 390% respectively). Serine 17-23 AKT serine/threonine kinase 1 Homo sapiens 13-16 24874604-6 2014 Interestingly, mutation of this serine to alanine (S106A), a non-phosphorylatable analogue, greatly diminished the ability of Asef2 to activate Rac, while a phosphomimetic mutation (serine to aspartic acid, S106D) enhanced Rac activation. Serine 32-38 AKT serine/threonine kinase 1 Homo sapiens 144-147 24874604-6 2014 Interestingly, mutation of this serine to alanine (S106A), a non-phosphorylatable analogue, greatly diminished the ability of Asef2 to activate Rac, while a phosphomimetic mutation (serine to aspartic acid, S106D) enhanced Rac activation. Serine 32-38 AKT serine/threonine kinase 1 Homo sapiens 223-226 24654606-7 2014 KEY RESULTS: Morphine stimulated Akt phosphorylation on Ser(473) and Thr(308) in a dose- and time-dependent manner indicating a functional mu-opioid receptor/Akt signalling pathway in mu-SK-N-LO cells. Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 158-161 24727557-5 2014 WJ1376-1 and WJ1398-1 also inhibited the TGF-beta-induced phosphorylation of Smad2 and of Akt at Ser 473, and the nuclear translocation of Smad2 was substantially lower in WJ1376-1- and WJ1398-1-treated cells than it was in control cells. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 90-93 24764294-7 2014 Akt phosphorylation activated eNOS (at Ser(1177)), and Akt also regulated the ability of Src to phosphorylate GIT1 as well as GIT1-eNOS association. Serine 39-42 AKT serine/threonine kinase 1 Homo sapiens 0-3 24792722-8 2014 These results suggest that betaPix phosphorylation at Ser-340 upregulates Nox1 through Rac activation, confirming Rac as a trigger for acute Nox1-dependent ROS production. Serine 54-57 AKT serine/threonine kinase 1 Homo sapiens 87-90 24522204-7 2014 Follow-up studies confirmed that AKT phosphorylates PHB2 on Ser-91 and that forced expression of the PHB2(S91A) mutant results in a rapid loss of viability and apoptotic cell death. Serine 60-63 AKT serine/threonine kinase 1 Homo sapiens 33-36 24851866-5 2014 By western blotting, we showed that the Akt/mTOR pathway, indicated by increased phosphorylation of Akt at serine 473 and S6 kinase 1 at threonine 389, was constitutively activated in hICD-expressing cells, similar to that in FPC knockdown cells and ARPKD kidneys. Serine 107-113 AKT serine/threonine kinase 1 Homo sapiens 40-43 24851866-5 2014 By western blotting, we showed that the Akt/mTOR pathway, indicated by increased phosphorylation of Akt at serine 473 and S6 kinase 1 at threonine 389, was constitutively activated in hICD-expressing cells, similar to that in FPC knockdown cells and ARPKD kidneys. Serine 107-113 AKT serine/threonine kinase 1 Homo sapiens 100-103 24365171-5 2014 RESULTS: After 14days of DDC treatment, there was an increase in periportal cells expressing FGFR1, FGFR2, and AKT-activated phospho-Serine 552 (pSer552) beta-Catenin in association with up-regulation of genes encoding the FGFR2IIIb ligands, Fgf7, Fgf10, and Fgf22. Serine 133-139 AKT serine/threonine kinase 1 Homo sapiens 111-114 24467442-1 2014 The insulin/IGF-1 (insulin-like growth factor 1)-activated protein kinase Akt (also known as protein kinase B) phosphorylates Ser487 in the "ST loop" (serine/threonine-rich loop) within the C-terminal domain of AMPK-alpha1 (AMP-activated protein kinase-alpha1), leading to inhibition of phosphorylation by upstream kinases at the activating site, Thr172. Serine 151-157 AKT serine/threonine kinase 1 Homo sapiens 74-77 24582688-1 2014 Our previous studies suggested that arsenic is able to induce serine 21 phosphorylation of the EZH2 protein through activation of JNK, STAT3, and Akt signaling pathways in the bronchial epithelial cell line, BEAS-2B. Serine 62-68 AKT serine/threonine kinase 1 Homo sapiens 146-149 24627641-13 2014 The resected tumor showed a heterogeneous reaction for phosphorylated Akt (Ser-473) by Western blotting and immunohistochemistry, indicating that parts of the tumor were sensitive to axitinib and other parts were not. Serine 75-78 AKT serine/threonine kinase 1 Homo sapiens 70-73 24535192-6 2014 At the same time, short-term exposure to arsenite induced a concentration-dependent increase in phosphorylation of Akt at Ser-473, followed by phosphorylation of FoxO proteins at sites known to be phosphorylated by Akt. Serine 122-125 AKT serine/threonine kinase 1 Homo sapiens 115-118 24523415-6 2014 First we showed that NMDA or K25 addition promoted a rapid stimulation of PI3K and a biphasic phosphorylation on Ser-473 of Akt, a PI3K substrate. Serine 113-116 AKT serine/threonine kinase 1 Homo sapiens 124-127 24446434-2 2014 AKT activation involves phosphorylation at two residues, Thr(308) and Ser(473), mediated by PDK1 and the mammalian target of rapamycin complex 2 (mTORC2), respectively. Serine 70-73 AKT serine/threonine kinase 1 Homo sapiens 0-3 24563481-9 2014 Together, our results suggest that AKT activated by FFSS directly phosphorylates Cx43 and integrin alpha5, and Ser-373 of Cx43 plays a predominant role in mediating the interaction between these two proteins and Cx43 hemichannel opening, a crucial step to mediate the anabolic function of mechanical loading in the bone. Serine 111-114 AKT serine/threonine kinase 1 Homo sapiens 35-38 24308965-7 2014 By overexpression of HSP27(15A) or HSP27(78/82A) phosphorylation mutant, we further showed that phosphorylation of HSP27 at serine 78/82 residues was essential to TRAIL-triggered Src-Akt/ERK signaling transduction. Serine 124-130 AKT serine/threonine kinase 1 Homo sapiens 183-186 24398676-14 2014 The conclusions were: 1) NHE3 basal activity is regulated by a signaling complex that is controlled by sequential effects of two kinases, Akt and GSK-3, which act on a Ser cluster in the same NHE3 C-terminal domain that binds ezrin; and 2) these kinases regulate the dynamic association of ezrin with NHE3 to affect basal NHE3 activity. Serine 168-171 AKT serine/threonine kinase 1 Homo sapiens 138-141 23748345-1 2014 Phosphorylation by Akt on Ser 280 was reported to induce cytoplasmic retention and inactivation of CHK1 with consequent genetic instability in PTEN-/- cells. Serine 26-29 AKT serine/threonine kinase 1 Homo sapiens 19-22 24525237-3 2014 Here, we show that downregulation of wild-type H-Ras or N-Ras in mutant K-Ras cancer cells leads to hyperactivation of the Erk/p90RSK and PI3K/Akt pathways and, consequently, the phosphorylation of Chk1 at an inhibitory site, Ser 280. Serine 226-229 AKT serine/threonine kinase 1 Homo sapiens 143-146 24337632-4 2014 Herein, expression of phosphorylated form of AKT (Ser 473) was investigated in HCC tumor (n=73), cirrhosis (n=17), normal liver (n=22) samples and in HCC cell lines (n=8). Serine 50-53 AKT serine/threonine kinase 1 Homo sapiens 45-48 24378649-7 2014 Furthermore, application of thrombin rescues beta-arrestin 1-lacking astrocytes from apoptosis by enhanced Akt (Ser 473) phosphorylation. Serine 112-115 AKT serine/threonine kinase 1 Homo sapiens 107-110 24378649-10 2014 Our most striking novel finding is that beta-arrestin 1 inhibits long-term thrombin-stimulated phosphorylation of Akt (Ser 473). Serine 119-122 AKT serine/threonine kinase 1 Homo sapiens 114-117 24378649-11 2014 This has been demonstrated by enhanced Akt (Ser 473) phosphorylation in astrocytes with knockdown of beta-arrestin 1. Serine 44-47 AKT serine/threonine kinase 1 Homo sapiens 39-42 24378649-13 2014 In addition, we also found that thrombin-induced phosphorylation of Akt (Ser 473) is increased by transactivation of the EGF and PDGF receptors in beta-arrestin 1-silenced astrocytes. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 68-71 24302004-4 2014 Exposure of HepG2 cells to excess amino acids reduced AMPK phosphorylation, upregulated Notch1 expression, and impaired the insulin-stimulated phosphorylation of Akt Ser(473) and insulin receptor substrate-1 (IRS-1) Tyr(612). Serine 166-169 AKT serine/threonine kinase 1 Homo sapiens 162-165 24479521-6 2014 RESULTS: The high Runx2 levels in invasive mammary epithelial cell lines promoted cell survival in Akt phosphorylation (pAkt-serine 473) dependent manner. Serine 125-131 AKT serine/threonine kinase 1 Homo sapiens 99-102 25171750-0 2014 Dipalmitoleoylphosphoethanolamine as a PP2A enhancer obstructs insulin signaling by promoting Ser/Thr dephosphorylation of Akt. Serine 94-97 AKT serine/threonine kinase 1 Homo sapiens 123-126 24309100-5 2014 AZD-2014 blocked activation of both mTORC1 (S6K and S6 phosphorylation) and mTORC2 (Akt Ser 473 phosphorylation), and activated autophagy in colorectal cancer cells. Serine 88-91 AKT serine/threonine kinase 1 Homo sapiens 84-87 25171750-7 2014 CONCLUSION: The results of the present study indicate that the PP2A enhancer DPPE obstructs insulin signaling by promoting serine/threonine dephosphorylation of Akt1/2, resulting in the suppression of GLUT4 translocation to the cell surface and glucose uptake into adipocytes. Serine 123-129 AKT serine/threonine kinase 1 Homo sapiens 161-167 24144700-4 2013 Knockdown of endogenous Rac or treatment of cells with a Rac-specific inhibitor decreases the amount of active MyoII, as determined by serine 19 (S19) phosphorylation, and negates the Asef2-promoted increase in contractility. Serine 135-141 AKT serine/threonine kinase 1 Homo sapiens 24-27 24365180-0 2013 DNA-dependent protein kinase catalytic subunit (DNA-PKcs)-SIN1 association mediates ultraviolet B (UVB)-induced Akt Ser-473 phosphorylation and skin cell survival. Serine 116-119 AKT serine/threonine kinase 1 Homo sapiens 112-115 24365180-1 2013 BACKGROUND: The exposure of skin keratinocytes to Ultraviolet (UV) irradiation leads to Akt phosphorylation at Ser-473, which is important for the carcinogenic effects of excessive sun exposure. Serine 111-114 AKT serine/threonine kinase 1 Homo sapiens 88-91 24365180-2 2013 The present study investigated the underlying mechanism of Akt Ser-473 phosphorylation by UVB radiation. Serine 63-66 AKT serine/threonine kinase 1 Homo sapiens 59-62 24365180-3 2013 RESULTS: We found that DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and mammalian target of rapamycin (mTOR) complex 2 (mTORC2) were both required for UVB-induced Akt Ser-473 phosphorylation in keratinocytes. Serine 180-183 AKT serine/threonine kinase 1 Homo sapiens 176-179 24365180-4 2013 Inhibition of DNA-PKcs activity via its inhibitor NU7026, a dominant-negative kinase-dead mutation, RNA interference (RNAi) or gene depletion led to the attenuation of UVB-induced Akt Ser-473 phosphorylation. Serine 184-187 AKT serine/threonine kinase 1 Homo sapiens 180-183 24365180-5 2013 Meanwhile, siRNA silencing or gene depletion of SIN1, a key component of mTORC2, abolished Akt Ser-473 phosphorylation by UVB. Serine 95-98 AKT serine/threonine kinase 1 Homo sapiens 91-94 24365180-6 2013 Significantly, we discovered that DNA-PKcs was associated with SIN1 in cytosol upon UVB radiation, and this complexation appeared required for Akt Ser-473 phosphorylation. Serine 147-150 AKT serine/threonine kinase 1 Homo sapiens 143-146 24365180-10 2013 CONCLUSION: Taken together, these results strongly suggest that DNA-PKcs-mTORC2 association is required for UVB-induced Akt Ser-473 phosphorylation and cell survival, and might be important for tumor cell transformation. Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 120-123 24144700-4 2013 Knockdown of endogenous Rac or treatment of cells with a Rac-specific inhibitor decreases the amount of active MyoII, as determined by serine 19 (S19) phosphorylation, and negates the Asef2-promoted increase in contractility. Serine 135-141 AKT serine/threonine kinase 1 Homo sapiens 57-60 23900743-9 2013 Levels of Akt phosphorylated at serine 473 declined with increasing doses of MK-2206, indicating successful Akt inhibition. Serine 32-38 AKT serine/threonine kinase 1 Homo sapiens 10-13 23958999-4 2013 Our results demonstrate that SNAP or l-arginine enhances AKT phosphorylation on both serine-473 and threonine-308 residues in a concentration and time-dependent manner. Serine 85-91 AKT serine/threonine kinase 1 Homo sapiens 57-60 24081328-1 2013 Our previous study showed that Akt phosphorylates TopBP1 at the Ser-1159 residue and induces its oligomerization. Serine 64-67 AKT serine/threonine kinase 1 Homo sapiens 31-34 24081328-8 2013 Hyperactivation of Akt inhibits Chk1 phosphorylation after hydroxyurea treatment, and this effect is dependent on TopBP1 phosphorylation at Ser-1159. Serine 140-143 AKT serine/threonine kinase 1 Homo sapiens 19-22 23872070-1 2013 mTORC2, the mammalian target of rapamycin complex 2 is activated by upstream growth factors, and performs two major functions, phosphorylation of AKT at the serine of 473 and cell cycle-dependent organization of actin cytoskeleton. Serine 157-163 AKT serine/threonine kinase 1 Homo sapiens 146-149 24093956-8 2013 Western blotting analysis indicated that LBH589 could decrease the expression of gankyrin and subsequently reduced serine-phosphorylated Akt and tyrosine-phosphorylated STAT3 expression although the total Akt and STAT3 were unaffected. Serine 115-121 AKT serine/threonine kinase 1 Homo sapiens 137-140 24055291-4 2013 Interestingly, we observed a decrease in expression of proteins that control the tumor micro environment such as VEGF-A, STAT-3, ERK1/2, ERK-p, AKT-1 ser 473 phosphorylation in compounds treated breast cancer cells. Serine 20-23 AKT serine/threonine kinase 1 Homo sapiens 144-149 24113173-6 2013 Proving the responsibility of the pathway, comparable effects were obtained with an Akt inhibitor (MK-2206); while suppressed phosphorylation of serine-184 may be attributed to reduced Akt activity itself, the causes of enhanced threonine-167 phosphorylation were addressed here. Serine 145-151 AKT serine/threonine kinase 1 Homo sapiens 84-87 24113173-6 2013 Proving the responsibility of the pathway, comparable effects were obtained with an Akt inhibitor (MK-2206); while suppressed phosphorylation of serine-184 may be attributed to reduced Akt activity itself, the causes of enhanced threonine-167 phosphorylation were addressed here. Serine 145-151 AKT serine/threonine kinase 1 Homo sapiens 185-188 23938603-8 2013 Rapamycin fully inhibited mTORC1 and partially inhibited mTORC2 activities, including the phosphorylation of Akt (serine 473) and PKCalpha, in vascular tumor cells. Serine 114-120 AKT serine/threonine kinase 1 Homo sapiens 109-112 24098385-3 2013 Angiotensin II caused attenuation of phosphorylated Akt (p-Akt), at serine 473; the p-Akt/Akt ratio decreased to 0.5+-0.2-fold the control value without angiotensin II (p<0.001). Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 52-55 24098385-3 2013 Angiotensin II caused attenuation of phosphorylated Akt (p-Akt), at serine 473; the p-Akt/Akt ratio decreased to 0.5+-0.2-fold the control value without angiotensin II (p<0.001). Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 59-62 24098385-3 2013 Angiotensin II caused attenuation of phosphorylated Akt (p-Akt), at serine 473; the p-Akt/Akt ratio decreased to 0.5+-0.2-fold the control value without angiotensin II (p<0.001). Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 59-62 24098385-3 2013 Angiotensin II caused attenuation of phosphorylated Akt (p-Akt), at serine 473; the p-Akt/Akt ratio decreased to 0.5+-0.2-fold the control value without angiotensin II (p<0.001). Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 59-62 23707764-6 2013 The elevated p27(kip1) expression levels were concurrent with the decrease of its phosphorylation at threonine 187 through suppression of CDK2 kinase activity and the reduced phosphorylation of Akt at Serine 473 by diminishing the kinase activity of the mammalian target of rapamycin (mTOR). Serine 201-207 AKT serine/threonine kinase 1 Homo sapiens 194-197 23940660-0 2013 Akt-induced phosphorylation of N-CoR at serine 1450 contributes to its misfolded conformational dependent loss (MCDL) in acute myeloid leukemia of the M5 subtype. Serine 40-46 AKT serine/threonine kinase 1 Homo sapiens 0-3 23991179-4 2013 Western blotting showed that the PP242 inhibition of mTORC2-mediated AKT phosphorylation at Ser 473 (AKT(S473)) was transient only in the first few hours of the PP242 treatment. Serine 92-95 AKT serine/threonine kinase 1 Homo sapiens 69-72 23991179-4 2013 Western blotting showed that the PP242 inhibition of mTORC2-mediated AKT phosphorylation at Ser 473 (AKT(S473)) was transient only in the first few hours of the PP242 treatment. Serine 92-95 AKT serine/threonine kinase 1 Homo sapiens 101-104 23843462-7 2013 The activated AKT phosphorylated MDM2 at Ser(166) and promoted degradation of the tumor suppressor p53. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 14-17 23940660-9 2013 Site directed mutagenic analysis of N-CoR identified serine 1450 as the crucial residue whose phosphorylation by Akt was essential for the misfolding and loss of N-CoR protein. Serine 53-59 AKT serine/threonine kinase 1 Homo sapiens 113-116 23623968-3 2013 In capacitated spermatozoa, progesterone transiently and concentration dependently lead to phosphorylation of AKT at both Thr 308 and Ser 473 in the tail region. Serine 134-137 AKT serine/threonine kinase 1 Homo sapiens 110-113 23922674-6 2013 PRINCIPAL FINDINGS: In DSRCT the PI3K/Akt/mTOR pathway is constitutively activated by p-Akt (Ser 473) expression in the nuclear compartment of the tumor cells and p-mTOR phosphorylated on Ser 2448, suggesting mTORC2 (rictor+mTOR) as the dominant form. Serine 93-96 AKT serine/threonine kinase 1 Homo sapiens 38-41 23922674-6 2013 PRINCIPAL FINDINGS: In DSRCT the PI3K/Akt/mTOR pathway is constitutively activated by p-Akt (Ser 473) expression in the nuclear compartment of the tumor cells and p-mTOR phosphorylated on Ser 2448, suggesting mTORC2 (rictor+mTOR) as the dominant form. Serine 93-96 AKT serine/threonine kinase 1 Homo sapiens 88-91 23922674-6 2013 PRINCIPAL FINDINGS: In DSRCT the PI3K/Akt/mTOR pathway is constitutively activated by p-Akt (Ser 473) expression in the nuclear compartment of the tumor cells and p-mTOR phosphorylated on Ser 2448, suggesting mTORC2 (rictor+mTOR) as the dominant form. Serine 188-191 AKT serine/threonine kinase 1 Homo sapiens 38-41 23922674-6 2013 PRINCIPAL FINDINGS: In DSRCT the PI3K/Akt/mTOR pathway is constitutively activated by p-Akt (Ser 473) expression in the nuclear compartment of the tumor cells and p-mTOR phosphorylated on Ser 2448, suggesting mTORC2 (rictor+mTOR) as the dominant form. Serine 188-191 AKT serine/threonine kinase 1 Homo sapiens 88-91 23497867-3 2013 Considering apoptosis-related factors, the polysaccharides suppressed AKT activity through the inhibition of AKT phosphorylation at Thr(308) and/or Ser(473). Serine 148-151 AKT serine/threonine kinase 1 Homo sapiens 70-73 23274913-7 2013 Overexpression of NYGGF4 significantly inhibited tyrosine phosphorylation of Insulin receptor substrate 1 (IRS-1) and serine phosphorylation of Akt, whereas overexpression of TFAM strongly induced phosphorylation of IRS-1 and Akt in NYGGF4-overexpressing adipocytes. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 144-147 23478261-7 2013 Hypoxia disrupts hnRNP E1/eNOS 3"-UTR interactions via increased Akt-mediated serine phosphorylation (including serine 43) and increased nuclear localization of hnRNP E1. Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 65-68 23478261-7 2013 Hypoxia disrupts hnRNP E1/eNOS 3"-UTR interactions via increased Akt-mediated serine phosphorylation (including serine 43) and increased nuclear localization of hnRNP E1. Serine 112-118 AKT serine/threonine kinase 1 Homo sapiens 65-68 23448267-2 2013 PDK1 is responsible for regulating the activity of related kinases in the AGC kinase family, including AKT, by phosphorylating a specific threonine or serine residue within the activation loop which is critical for kinase activation. Serine 151-157 AKT serine/threonine kinase 1 Homo sapiens 103-106 23457334-9 2013 In accordance with these results, kinase assays showed that amino acid-activated Akt phosphorylated PFKFB2 at Ser-483 and that knockdown experiments confirmed that the increase in Fru-2,6-P2 concentration induced by amino acids was due to PFKFB2. Serine 110-113 AKT serine/threonine kinase 1 Homo sapiens 81-84 23596468-5 2013 Knockdown of PODXL in U-87 cells significantly decreased cell invasion, MMP-9 expression, cell survival against temozolomide, and phosphorylation of Akt at serine 473 (ser473), which was further decreased by LY treatment. Serine 156-162 AKT serine/threonine kinase 1 Homo sapiens 149-152 23533049-10 2013 NEL suppressed insulin-induced phosphorylation of both Akt and eNOS at serine residues 473 and 1177, respectively. Serine 71-77 AKT serine/threonine kinase 1 Homo sapiens 55-58 23466053-7 2013 The phosphorylation level of Akt (Ser 473) and the expression level of mTOR increased in IDM fed the fish oil diet compared with those fed the lard diet (P < 0.05). Serine 34-37 AKT serine/threonine kinase 1 Homo sapiens 29-32 23421998-6 2013 Akt phosphorylates p57 on Ser 282 or Thr310. Serine 26-29 AKT serine/threonine kinase 1 Homo sapiens 0-3 23223135-0 2013 mTORC2-PKBalpha/Akt1 Serine 473 phosphorylation axis is essential for regulation of FOXP3 Stability by chemokine CCL3 in psoriasis. Serine 21-27 AKT serine/threonine kinase 1 Homo sapiens 7-15 22976217-10 2013 B potentiated phosphorylation (relative to P) of Akt (Ser(473)) and p70 S6 k (Thr(389)) (p = 0.016 and p = 0.005, respectively). Serine 54-57 AKT serine/threonine kinase 1 Homo sapiens 49-52 23255608-3 2013 Our data indicate that exposure of pulmonary arterial endothelial cells to ADMA enhanced eNOS phosphorylation at the Akt1-dependent phosphorylation sites Ser(617) and Ser(1179). Serine 154-157 AKT serine/threonine kinase 1 Homo sapiens 117-121 23255608-3 2013 Our data indicate that exposure of pulmonary arterial endothelial cells to ADMA enhanced eNOS phosphorylation at the Akt1-dependent phosphorylation sites Ser(617) and Ser(1179). Serine 167-170 AKT serine/threonine kinase 1 Homo sapiens 117-121 23255608-11 2013 In conclusion, we have identified a new mechanism by which eNOS translocation to mitochondria is regulated by the phosphorylation of eNOS at Ser(617) and Ser(1179) by Akt1 and that this is enhanced when Akt1 becomes nitrated at Tyr(350). Serine 141-144 AKT serine/threonine kinase 1 Homo sapiens 167-171 23255608-11 2013 In conclusion, we have identified a new mechanism by which eNOS translocation to mitochondria is regulated by the phosphorylation of eNOS at Ser(617) and Ser(1179) by Akt1 and that this is enhanced when Akt1 becomes nitrated at Tyr(350). Serine 141-144 AKT serine/threonine kinase 1 Homo sapiens 203-207 23255608-11 2013 In conclusion, we have identified a new mechanism by which eNOS translocation to mitochondria is regulated by the phosphorylation of eNOS at Ser(617) and Ser(1179) by Akt1 and that this is enhanced when Akt1 becomes nitrated at Tyr(350). Serine 154-157 AKT serine/threonine kinase 1 Homo sapiens 167-171 23255608-11 2013 In conclusion, we have identified a new mechanism by which eNOS translocation to mitochondria is regulated by the phosphorylation of eNOS at Ser(617) and Ser(1179) by Akt1 and that this is enhanced when Akt1 becomes nitrated at Tyr(350). Serine 154-157 AKT serine/threonine kinase 1 Homo sapiens 203-207 23426781-4 2013 In Saos-2 cells, overexpression of TWIST significantly decreased ET-1 mRNA and protein expression levels, cell survival against cisplatin and phosphorylation of Akt at serine 473 (ser473), which was abolished by the selective phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002, or the selective ETAR inhibitor, BQ123. Serine 168-174 AKT serine/threonine kinase 1 Homo sapiens 161-164 24024143-6 2013 A time- and concentration-dependent increase in phosphorylation of FoxO1a and FoxO3a at sites known to be phosphorylated by Akt as well as phosphorylation of Akt at Ser-473 was detected. Serine 165-168 AKT serine/threonine kinase 1 Homo sapiens 158-161 23223135-0 2013 mTORC2-PKBalpha/Akt1 Serine 473 phosphorylation axis is essential for regulation of FOXP3 Stability by chemokine CCL3 in psoriasis. Serine 21-27 AKT serine/threonine kinase 1 Homo sapiens 16-20 23223135-8 2013 This degradation was mainly dependent on upregulation of Serine 473 phosphorylation of the PKBalpha/Akt1 isoform, and almost independent of mTORC1 (mammalian target of rapamycin complex 1) activity. Serine 57-63 AKT serine/threonine kinase 1 Homo sapiens 91-99 23223135-8 2013 This degradation was mainly dependent on upregulation of Serine 473 phosphorylation of the PKBalpha/Akt1 isoform, and almost independent of mTORC1 (mammalian target of rapamycin complex 1) activity. Serine 57-63 AKT serine/threonine kinase 1 Homo sapiens 100-104 23275565-5 2013 Degradation is initiated by phosphorylation of p300 at serine 1834, which is catalyzed by the cooperative action of p38 mitogen-activated protein kinases and Akt kinases. Serine 55-61 AKT serine/threonine kinase 1 Homo sapiens 158-161 24094245-6 2013 Moreover, DNA-PKcs and phosphorylated Akt at Ser(473) proved to be crucial in regulating the Nrf2-ARE pathway. Serine 45-48 AKT serine/threonine kinase 1 Homo sapiens 38-41 23072587-9 2013 RESULTS: OxLDL impaired T(3)-mediated AKT phosphorylation at serine 473 and significantly decreased the production of both NO (oxLDL+T(3) vs. T(3), 9.79+-0.5 AU vs. 80.75+-2.8 AU, mean+-standard deviation, p<0.0001) and cGMP. Serine 61-67 AKT serine/threonine kinase 1 Homo sapiens 38-41 22349818-6 2013 Mutation of CBP Thr-1872 or Mdm2 Ser-186/188 Akt sites resulted in a dissociation of the ERbeta-CBP-Mdm2 complex and reduced ERbeta turnover. Serine 33-36 AKT serine/threonine kinase 1 Homo sapiens 45-48 23147412-6 2013 Treatment with MK-2206 effectively suppressed AKT phosphorylation at serine 473 and significantly reduced cell proliferation in a dose-dependent manner. Serine 69-75 AKT serine/threonine kinase 1 Homo sapiens 46-49 23437362-5 2013 Rapamycin caused a striking increase in Akt phosphorylation at Ser(473) while the active-site inhibitors of mTOR (KU63794 and PP242) completely abrogated Akt phosphorylation at this site. Serine 63-66 AKT serine/threonine kinase 1 Homo sapiens 40-43 23437362-8 2013 In contrast, metformin abolished mTORC1 activation without over-stimulating Akt phosphorylation on Ser(473) and prevented mitogen-stimulated ERK activation in PDAC cells. Serine 99-102 AKT serine/threonine kinase 1 Homo sapiens 76-79 23603888-2 2013 Upon insulin activation of cellular Akt, the enzyme multi-phosphorylated a single serine residue of a diserine DCF substrate in a time-dependent manner, culminating in monophospho- to triphospho-serine products. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 36-39 23603888-2 2013 Upon insulin activation of cellular Akt, the enzyme multi-phosphorylated a single serine residue of a diserine DCF substrate in a time-dependent manner, culminating in monophospho- to triphospho-serine products. Serine 104-110 AKT serine/threonine kinase 1 Homo sapiens 36-39 22968630-4 2012 NYGGF4 overexpression resulted in significant inhibition of tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt, whereas incubation with metformin strongly activated IRS-1 and Akt phosphorylation in NYGGF4 overexpression adipocytes. Serine 98-104 AKT serine/threonine kinase 1 Homo sapiens 124-127 23030823-2 2012 Akt is activated by phosphorylation of its T-loop residue (Thr(308)) by PDK1 (3-phosphoinositide-dependent kinase-1) and its C-terminal hydrophobic motif (Ser(473)) by mTORC2 [mTOR (mammalian target of rapamycin) complex 2]. Serine 155-158 AKT serine/threonine kinase 1 Homo sapiens 0-3 23030823-7 2012 The second entails recruitment of PDK1 to Akt after its phosphorylation at Ser(473) by mTORC2, via a substrate-docking motif termed the PIF-pocket. Serine 75-78 AKT serine/threonine kinase 1 Homo sapiens 42-45 23030823-9 2012 These findings suggest that suppression of Ser(473) phosphorylation by using mTOR inhibitors would disrupt the PIF-pocket mechanism and thereby sensitize Akt to PDK1 inhibitors. Serine 43-46 AKT serine/threonine kinase 1 Homo sapiens 154-157 23035229-8 2012 Infected hepatocytes had significantly increased levels of phosphorylated beta-catenin (Ser(552)) as an EMT mediator, which translocated into the nucleus and activated Akt. Serine 88-91 AKT serine/threonine kinase 1 Homo sapiens 168-171 23131847-2 2012 One of these mechanisms is the phosphorylation of the serine and threonine kinase Akt at the cytosolic side of the plasma membrane by phosphoinositide-dependent protein kinase 1 (PDK1), which is recruited and activated by binding to the phosphoinositides produced by PI3K. Serine 54-60 AKT serine/threonine kinase 1 Homo sapiens 82-85 23095642-5 2012 Mechanistic studies show that Akt causes CSN6 phosphorylation at Ser 60, which, in turn, reduces ubiquitin-mediated protein degradation of CSN6. Serine 65-68 AKT serine/threonine kinase 1 Homo sapiens 30-33 23085536-6 2012 Silencing also diminished insulin-stimulated tyrosinephosphorylation of IRS-1 and serine phosphorylation of Akt. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 108-111 22962253-6 2012 Moreover, E(2) through phosphatidylinositol 3 kinase/Akt activation caused a significant decrease of PTP1B catalytic activity along with an increase in its serine phosphorylation. Serine 156-162 AKT serine/threonine kinase 1 Homo sapiens 53-56 22771387-6 2012 Akt phosphorylation at serine 473 correlated with JNK activity, and co-immunoprecipitation studies revealed a direct interaction between JNK and Akt. Serine 23-29 AKT serine/threonine kinase 1 Homo sapiens 0-3 22458600-9 2012 It was also discovered S100A4 cDNA induced protein kinase B (AKT) phosphorylation at Serine-473(phospho-AKT [p-AKT]) levels, followed by the Slug upregulation, and S100A4 siRNA decreases the phospho-AKT levels, followed by the Slug downregulation. Serine 85-91 AKT serine/threonine kinase 1 Homo sapiens 61-64 23045700-7 2012 PI3K activation leads to activation of AKT through phosphorylation of AKT on Thr(308) and Ser(473). Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 39-42 22766511-7 2012 In addition, TAE226 inhibited the expression of phospho-FAK Tyr(397) and phospho AKT Ser(473), resulting in caspase-mediated apoptosis. Serine 85-88 AKT serine/threonine kinase 1 Homo sapiens 81-84 22820890-5 2012 These changes were accompanied by a marked upregulation in expression of insulin-stimulated tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt following treatment with alpha-LA. Serine 130-136 AKT serine/threonine kinase 1 Homo sapiens 156-159 22927331-5 2012 CXCL16 increased Akt phosphorylation (Thr(308)/Ser(473)), an effect abrogated by phosphatidylinositide 3-kinase inhibitors wortmannin (100 nmol/L) and LY294002 (25 micromol/L). Serine 47-50 AKT serine/threonine kinase 1 Homo sapiens 17-20 22307544-2 2012 Although phosphatidylinositol-3-OH kinase (PI3K), a lipid kinase, is well characterized as a major regulator of AKT activation in response to a variety of ligands, recent studies highlight a diverse group of tyrosine (Ack1/TNK2, Src, PTK6) and serine/threonine (TBK1, IKBKE, DNAPKcs) kinases that activate AKT directly to promote its pro-proliferative signaling functions. Serine 244-250 AKT serine/threonine kinase 1 Homo sapiens 112-115 22644784-8 2012 This is supported by the following data: (1) IL-1beta induces 473 serine phosphorylation of Akt; (2) IL-1beta-mediated Akt activation occurs in a PI-3K-dependent manner, and specific inhibition of PI-3K prevents Akt phosphorylation; and (3) inhibition of Akt prevents IL-1beta-mediated DEC1 and HIF-1alpha induction. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 119-122 22644784-8 2012 This is supported by the following data: (1) IL-1beta induces 473 serine phosphorylation of Akt; (2) IL-1beta-mediated Akt activation occurs in a PI-3K-dependent manner, and specific inhibition of PI-3K prevents Akt phosphorylation; and (3) inhibition of Akt prevents IL-1beta-mediated DEC1 and HIF-1alpha induction. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 119-122 22904301-8 2012 Our results demonstrate that Akt1 signals through beta-catenin by phosphorylation on Ser(552), a site that differs from the glycogen synthase kinase 3 beta phosphorylation site. Serine 85-88 AKT serine/threonine kinase 1 Homo sapiens 29-33 22904301-9 2012 Stimulation of a chemically activated version of Akt1, in the absence of other TLR3-dependent signaling, was sufficient for accumulation and phosphorylation of beta-catenin at Ser(552). Serine 176-179 AKT serine/threonine kinase 1 Homo sapiens 49-53 22644784-8 2012 This is supported by the following data: (1) IL-1beta induces 473 serine phosphorylation of Akt; (2) IL-1beta-mediated Akt activation occurs in a PI-3K-dependent manner, and specific inhibition of PI-3K prevents Akt phosphorylation; and (3) inhibition of Akt prevents IL-1beta-mediated DEC1 and HIF-1alpha induction. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 92-95 22644784-8 2012 This is supported by the following data: (1) IL-1beta induces 473 serine phosphorylation of Akt; (2) IL-1beta-mediated Akt activation occurs in a PI-3K-dependent manner, and specific inhibition of PI-3K prevents Akt phosphorylation; and (3) inhibition of Akt prevents IL-1beta-mediated DEC1 and HIF-1alpha induction. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 119-122 22685298-3 2012 Here, we show that mutation of Ser-1248 (S1248A) enhances IGF-1R in vitro kinase activity, autophosphorylation, Akt/mammalian target of rapamycin activity, and cell growth. Serine 31-34 AKT serine/threonine kinase 1 Homo sapiens 112-115 22767580-7 2012 Conversely, up-regulation of ADAM12L induces the Akt Ser-473 phosphorylation-dependent survival pathway via stimulation of beta1 integrins and activation of phosphoinositide 3-kinase (PI3K). Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 49-52 22766331-9 2012 The beneficial modulation of serine/tyrosine phosphorylation of IRS-1 by diosgenin contributed to the improvement of insulin signaling along PI3K/Akt/eNOS pathways and thereby increased insulin-mediated NO production. Serine 29-35 AKT serine/threonine kinase 1 Homo sapiens 146-149 22817723-6 2012 This pathway is aberrantly overactivated in AD brain at the level of increased activation of the serine/threonine kinase Akt and the phosphorylation of its downstream targets, including mTOR (mammalian target of rapamycin). Serine 97-103 AKT serine/threonine kinase 1 Homo sapiens 121-124 22496446-4 2012 Increasing Ser-9 phosphorylation of GSK-3beta by inhibiting phosphatase 1/2A or pretreating with purified active Akt inhibited calpain-mediated cleavage of GSK-3beta at both N and C termini, whereas non-phosphorylatable mutant GSK-3beta S9A facilitated its cleavage. Serine 11-14 AKT serine/threonine kinase 1 Homo sapiens 113-116 22781553-7 2012 We show for the first time that induction of AKT phosphorylation at serine 473 by AT13148, as reported for other ATP-competitive inhibitors of AKT, is not a therapeutically relevant reactivation step. Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 45-48 22759359-13 2012 CONCLUSIONS: Collectively, these data suggest that phosphorylation of TG2 at Ser(216) plays a role in TG2 mediated activation of NF-kappaB, Akt and in the downregulation of PTEN. Serine 77-80 AKT serine/threonine kinase 1 Homo sapiens 140-143 21840189-6 2012 Antroquinonol inhibited Akt phosphorylation at Ser(473), the phosphorylation site critical for Akt kinase activity, and blocked the mammalian target of rapamycin (mTOR) phosphorylation at Ser(2448), a site dependent on mTOR activity. Serine 47-50 AKT serine/threonine kinase 1 Homo sapiens 24-27 21840189-6 2012 Antroquinonol inhibited Akt phosphorylation at Ser(473), the phosphorylation site critical for Akt kinase activity, and blocked the mammalian target of rapamycin (mTOR) phosphorylation at Ser(2448), a site dependent on mTOR activity. Serine 188-191 AKT serine/threonine kinase 1 Homo sapiens 24-27 22707636-5 2012 In addition, TNF-alpha treatment inhibited glycogen synthase kinase 3beta (GSK-3beta) activity through serine-9 phosphorylation mediated by the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway in RCC cells. Serine 103-109 AKT serine/threonine kinase 1 Homo sapiens 197-200 22492939-5 2012 At 1 h postexercise, phosphorylation of Akt on Ser(473) (CE = 7.7; RE = 4.6) and Thr(308) (CE = 4.4; RE = 2.9), and PRAS40 on Thr(246) (CE = 3.8; AE = 2.5) increased (P < 0.05), with CE greater than AE for Akt Ser(473)-Thr(308) and greater than RE for PRAS40 (P < 0.05). Serine 47-50 AKT serine/threonine kinase 1 Homo sapiens 40-43 22492939-5 2012 At 1 h postexercise, phosphorylation of Akt on Ser(473) (CE = 7.7; RE = 4.6) and Thr(308) (CE = 4.4; RE = 2.9), and PRAS40 on Thr(246) (CE = 3.8; AE = 2.5) increased (P < 0.05), with CE greater than AE for Akt Ser(473)-Thr(308) and greater than RE for PRAS40 (P < 0.05). Serine 213-216 AKT serine/threonine kinase 1 Homo sapiens 40-43 21996732-4 2012 TOPK concurrently promoted AKT phosphorylation at Ser(473) and decreased the phosphatase and tensin homolog (PTEN) levels, whereas TOPK knockdown had the reverse effects. Serine 50-53 AKT serine/threonine kinase 1 Homo sapiens 27-30 22366458-5 2012 We also show that Akt-mediated phosphorylation at serine 227 is necessary for directing nuclear translocation of hTERT. Serine 50-56 AKT serine/threonine kinase 1 Homo sapiens 18-21 22431710-6 2012 Notably, parkin-expressing glioma cells showed a reduction in levels of cyclin D1, but not cyclin E, and a selective downregulation of Akt serine-473 phosphorylation and VEGF receptor levels. Serine 139-145 AKT serine/threonine kinase 1 Homo sapiens 135-138 22550181-3 2012 We provide evidence that mTORC2 complexes control IFN-induced phosphorylation of AKT on serine 473 and their function is ultimately required for IFN-dependent gene transcription via interferon-stimulated response elements. Serine 88-94 AKT serine/threonine kinase 1 Homo sapiens 81-84 22173835-9 2012 Silencing Rictor gene expression by RNAi significantly suppressed 8-CPT-2Me-cAMP-induced phosphorylation of Akt at Ser(473) . Serine 115-118 AKT serine/threonine kinase 1 Homo sapiens 108-111 22569334-1 2012 The protein serine-threonine kinase Akt undergoes a substantial conformational change upon activation, which is induced by the phosphorylation of two critical regulatory residues, threonine 308 and serine 473. Serine 12-18 AKT serine/threonine kinase 1 Homo sapiens 36-39 22286127-7 2012 NBP increased the phosphorylation of Akt and eNOS at serine 1177, which was blocked by LY294002. Serine 53-59 AKT serine/threonine kinase 1 Homo sapiens 37-40 22309289-3 2012 Here we show that HBx interacts with Akt1 kinase and is phosphorylated at serine 31 as indicated by mutational analysis of the Akt recognition motif (creating the HBxS31A mutant) or immunoblotting of HBx immunoprecipitates using Akt motif-specific antibody. Serine 74-80 AKT serine/threonine kinase 1 Homo sapiens 37-41 22309289-3 2012 Here we show that HBx interacts with Akt1 kinase and is phosphorylated at serine 31 as indicated by mutational analysis of the Akt recognition motif (creating the HBxS31A mutant) or immunoblotting of HBx immunoprecipitates using Akt motif-specific antibody. Serine 74-80 AKT serine/threonine kinase 1 Homo sapiens 127-130 22801313-10 2012 CONCLUSION: Genistein could promote the activity of eNOS through increasing phosphorylation eNOS(Ser(1179)) level through PI3K/AKT pathway. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 127-130 22334668-6 2012 Akt phosphorylates PHF20 at Ser(291) in vitro and in vivo, which results in its translocation from the nucleus to the cytoplasm and attenuation of PHF20 function. Serine 28-31 AKT serine/threonine kinase 1 Homo sapiens 0-3 22801313-7 2012 The effect of genistein on phosphorylation eNOS(Ser(1179)) level was also observed in the presence of LY294002 or NSC154020 (PI3K and AKT inhibitors). Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 134-137 22801313-9 2012 Furthermore, the expression level of phosphorylation eNOS(Ser(1179)) was significantly lower in PIK3/AKT inhibitors LY294002 and NSC154020 treated cells compared with ox-LDL + genistein treated cells (all P < 0.05). Serine 58-61 AKT serine/threonine kinase 1 Homo sapiens 101-104 22371971-4 2012 In this study, we report that calcineurin, a Ca2+/calmodulin-dependent serine (ser)/threonine (thr) phosphatase, participates in the circadian output pathway to regulate L-VGCCs through modulating both Ras-MAPK and Ras-PI3K-AKT signaling. Serine 71-77 AKT serine/threonine kinase 1 Homo sapiens 224-227 22280833-13 2012 Stimulation of prostate tissues with noradrenaline (30 muM, n=8 patients) or phenylephrine (10 muM, n=7 patients) caused significant Akt phosphorylation at serine-473, indicating activation of Akt. Serine 156-162 AKT serine/threonine kinase 1 Homo sapiens 133-136 22204954-6 2012 We measured a 36% decrease in phosphorylation of Akt Ser(473) during ischemia and 37% during reperfusion (P < 0.05). Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 49-52 22371971-4 2012 In this study, we report that calcineurin, a Ca2+/calmodulin-dependent serine (ser)/threonine (thr) phosphatase, participates in the circadian output pathway to regulate L-VGCCs through modulating both Ras-MAPK and Ras-PI3K-AKT signaling. Serine 71-74 AKT serine/threonine kinase 1 Homo sapiens 224-227 21818627-7 2012 Akt and NF-kappaB related inflammatory cytokine IL-1alpha, and IL-6 and the chemokine IL-8 were upregulated in treated cells at 6 and 24 h. The calpain inhibitor leupeptin limited Akt phosphorylation to Ser(473) and reduced release of IL-1alpha, IL-6, and IL-8, indicating that calpain or similar protease(s) are involved in PM-induced activation of Akt and subsequent release of inflammatory cytokines. Serine 203-206 AKT serine/threonine kinase 1 Homo sapiens 0-3 22142888-10 2012 Furthermore, Chloroquine was able to block the RAD001-induced phosphorylation of Akt serine 473. Serine 85-91 AKT serine/threonine kinase 1 Homo sapiens 81-84 22184107-12 2012 Resistance of Ser-473, but not Thr-308, phosphorylation of AKT to PI3K inhibitors suggested an activation of mTORC2 by NS5A. Serine 14-17 AKT serine/threonine kinase 1 Homo sapiens 59-62 22290291-5 2012 The concomitant inactivation of Akt and MAP kinases in response to DIM facilitated the dephosphorylation of the proapoptotic protein Bad at Ser-136 and Ser-112, respectively. Serine 140-143 AKT serine/threonine kinase 1 Homo sapiens 32-35 22290291-5 2012 The concomitant inactivation of Akt and MAP kinases in response to DIM facilitated the dephosphorylation of the proapoptotic protein Bad at Ser-136 and Ser-112, respectively. Serine 152-155 AKT serine/threonine kinase 1 Homo sapiens 32-35 22262182-1 2012 AGC kinases, including the three Akt (protein kinase B) isoforms, protein kinase A (PKA) and all protein kinase C (PKC) isoforms, require activation loop phosphorylation (threonine 308 in Akt1) as well as phosphorylation of a C-terminal residue (serine 473 in Akt1) for catalytic activity and phosphorylation of downstream targets. Serine 246-252 AKT serine/threonine kinase 1 Homo sapiens 33-36 22262182-1 2012 AGC kinases, including the three Akt (protein kinase B) isoforms, protein kinase A (PKA) and all protein kinase C (PKC) isoforms, require activation loop phosphorylation (threonine 308 in Akt1) as well as phosphorylation of a C-terminal residue (serine 473 in Akt1) for catalytic activity and phosphorylation of downstream targets. Serine 246-252 AKT serine/threonine kinase 1 Homo sapiens 188-192 21973116-4 2012 Leptin activates endothelial NO synthase (eNOS) through a mechanism involving AMP-activated protein kinase (AMPK) and protein kinase B/Akt, which phosphorylates eNOS at Ser(1177) , increasing its activity. Serine 169-172 AKT serine/threonine kinase 1 Homo sapiens 135-138 21763075-5 2012 We measured by Western blot the phosphorylated forms of Akt at serine 473 and threonine 308, GSK3beta at serine 9 and tyrosine 216 and GSK3alpha at serine 21 in anterior, middle and posterior caudate nucleus and putamen. Serine 63-69 AKT serine/threonine kinase 1 Homo sapiens 56-59 22084251-2 2012 A key step in Akt activation is phosphorylation at Ser-473 by the mammalian target of rapamycin (mTOR) complex 2 (mTORC2). Serine 51-54 AKT serine/threonine kinase 1 Homo sapiens 14-17 21743495-9 2012 A single 14-3-3-binding motif encompassing serine 83 on p85 is largely responsible for 14-3-3zeta-mediated p85 binding and PI3K/Akt activation. Serine 43-49 AKT serine/threonine kinase 1 Homo sapiens 128-131 22012613-10 2012 Indeed, our results showed that the phosphorylation of Akt at Thr 308 in the endothelial cells and phosphorylation of Akt at Ser 473 in adventitia was decreased in GK rats, compared with Wistar control. Serine 125-128 AKT serine/threonine kinase 1 Homo sapiens 118-121 22911754-8 2012 A decrease in the basal activity of c-Src (phospho-Tyr-416), focal adhesion kinase (phospho-Tyr-397), and Akt (phospho-Ser-473) was also detected following eEF-2K down-regulation in MDA-MB-231 cells, as determined by Western blotting. Serine 119-122 AKT serine/threonine kinase 1 Homo sapiens 106-109 21879255-12 2012 The activation of Akt (Ser 473) and Erk (Thr202/Tyr204) by radiation was both dose- and time-dependent. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 18-21 22293289-4 2012 Insulin increased the Ser(473) phosphorylation of Akt. Serine 22-25 AKT serine/threonine kinase 1 Homo sapiens 50-53 22912850-11 2012 IGF-1-dependent phosphorylation of serine 573 was reduced by inhibition of PI 3-kinase and Akt/PKB. Serine 35-41 AKT serine/threonine kinase 1 Homo sapiens 91-98 22905202-5 2012 Treatment of Miapaca-2 and PANC-1 cells with GSPs also decreased the levels of phosphatidylinositol-3-kinase (PI3K) and phosphorylation of Akt at ser(473). Serine 146-149 AKT serine/threonine kinase 1 Homo sapiens 139-142 22194727-6 2011 Pharmacological inhibition of Akt, the kinase responsible for Ser 1177 phosphorylation, attenuated the stimulatory effect of H(2)S on NO production. Serine 62-65 AKT serine/threonine kinase 1 Homo sapiens 30-33 22536436-6 2012 Akt phosphorylation at Ser-473 (pAkt) is commonly used as a marker for assessing IR. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 0-3 22023045-5 2011 However, very recent reports from our laboratory and others demonstrate that AKT can also directly regulate FAK through direct association and serine phosphorylation. Serine 143-149 AKT serine/threonine kinase 1 Homo sapiens 77-80 21664164-6 2011 RESULTS: Compared with cells from non-diabetic subjects, adipocytes from T2DM patients showed signs of insulin resistance, with significantly reduced IRS-1 tyrosine and AKT (Ser 473) phosphorylation levels in response to insulin stimulation. Serine 174-177 AKT serine/threonine kinase 1 Homo sapiens 169-172 21937042-4 2011 In addition we measured eNOS and Akt activation through the evaluation of their phosphorylation at Ser(1177) and Ser(473) sites respectively. Serine 99-102 AKT serine/threonine kinase 1 Homo sapiens 33-36 21908613-1 2011 The pro-survival kinase Akt requires phosphorylation at two conserved residues, the activation loop site (Thr-308) and the hydrophobic motif site (Ser-473), for maximal activation. Serine 147-150 AKT serine/threonine kinase 1 Homo sapiens 24-27 22000207-5 2011 These compounds also exhibited inhibition of NF-kappaB as well as its downstream target gene Akt1 and the phosphorylated form of AKt ser 474 proteins. Serine 133-136 AKT serine/threonine kinase 1 Homo sapiens 129-132 21904110-6 2011 Treatment with TNF-alpha resulted in a strong increase in the phosphorylation of Akt on Ser-473 and when cells were treated with phosphatidylinositol (PI) 3-kinase inhibitors such as LY294002 or wortmannin, or Akt inhibitor (Akt inhibitor IV), induction of STS mRNA expression by TNF-alpha was significantly prevented. Serine 88-91 AKT serine/threonine kinase 1 Homo sapiens 81-84 21965657-3 2011 The PI3K-dependent signaling kinase complex mTORC2 (mammalian target of rapamycin complex 2) has been defined as the regulatory Ser-473 kinase of Akt. Serine 128-131 AKT serine/threonine kinase 1 Homo sapiens 146-149 21965657-6 2011 We show that the functional mTOR kinase domain is required for the mTORC2 activity as the Ser-473 kinase of Akt. Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 108-111 21908613-3 2011 Here we show that Akt can be phosphorylated at the hydrophobic motif site (Ser-473) in the absence of mTORC2. Serine 75-78 AKT serine/threonine kinase 1 Homo sapiens 18-21 21908613-6 2011 Second, constructs of Akt in which the PH domain is constitutively disengaged from the kinase domain are phosphorylated at the hydrophobic motif site in Sin1(-/-) MEFs; both myristoylated-Akt and Akt lacking the PH domain are phosphorylated at Ser-473. Serine 244-247 AKT serine/threonine kinase 1 Homo sapiens 22-25 21908613-8 2011 In summary, these data support a model in which Akt can be phosphorylated at Ser-473 and activated in the absence of mTORC2 by mechanisms that depend on removal of the PH domain from the kinase domain. Serine 77-80 AKT serine/threonine kinase 1 Homo sapiens 48-51 21741919-13 2011 DARPP-32-mediated resistance to gefitinib resulted from increased phosphorylation of and interaction between EGFR and ERBB3, which led to phosphorylation of AKT (at serine 473). Serine 165-171 AKT serine/threonine kinase 1 Homo sapiens 157-160 21956113-0 2011 Mechanical regulation of glycogen synthase kinase 3beta (GSK3beta) in mesenchymal stem cells is dependent on Akt protein serine 473 phosphorylation via mTORC2 protein. Serine 121-127 AKT serine/threonine kinase 1 Homo sapiens 109-112 21956113-4 2011 Mechanical strain (2% magnitude, 0.17 Hz) induced phosphorylation of Akt at Ser-473 and Thr-308 in parallel with phosphorylation of GSK3beta at Ser-9. Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 69-72 21956113-5 2011 Inhibiting Akt (Akt1/2 kinase inhibitor treatment or Akt knockdown) prevented strain-induced phosphorylation of GSK3beta at Ser-9. Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 11-14 21956113-5 2011 Inhibiting Akt (Akt1/2 kinase inhibitor treatment or Akt knockdown) prevented strain-induced phosphorylation of GSK3beta at Ser-9. Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 16-19 21956113-7 2011 As Rictor/mTORC2 (mammalian target of rapamycin complex 2) is known to transduce phosphorylation of Akt at Ser-473 by insulin, we investigated whether it contributes to strain-induced Ser-473 phosphorylation. Serine 107-110 AKT serine/threonine kinase 1 Homo sapiens 100-103 21956113-10 2011 In sum, mechanical input initiates a signaling cascade that is uniquely dependent on mTORC2 activation and phosphorylation of Akt at Ser-473, an effect sufficient to cause inactivation of GSK3beta. Serine 133-136 AKT serine/threonine kinase 1 Homo sapiens 126-129 21956113-11 2011 Thus, mechanical regulation of GSK3beta downstream of Akt is dependent on phosphorylation of Akt at Ser-473 in a manner distinct from that of growth factors. Serine 100-103 AKT serine/threonine kinase 1 Homo sapiens 54-57 21956113-11 2011 Thus, mechanical regulation of GSK3beta downstream of Akt is dependent on phosphorylation of Akt at Ser-473 in a manner distinct from that of growth factors. Serine 100-103 AKT serine/threonine kinase 1 Homo sapiens 93-96 21705328-9 2011 One pathway involves PKC-alpha catalyzed phosphorylation of CREB and the other involves a PKC-mediated the PP2A catalyzed dephosphorylation of Akt at Ser(473) which in turn leads to FOXO3a Ser(253) dephosphorylation and its activation. Serine 150-153 AKT serine/threonine kinase 1 Homo sapiens 143-146 21823616-3 2011 Compound 22 facilitated the dephosphorylation of Akt at Ser-473 and other ILK targets, including glycogen synthase kinase-3beta and myosin light chain. Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 49-52 21741389-8 2011 Agonist-induced phosphorylation of eNOS on serine 1177 was attenuated by Akt inhibition, whereas association of eNOS with actin was not. Serine 43-49 AKT serine/threonine kinase 1 Homo sapiens 73-76 21741389-10 2011 We conclude that association of globular actin with eNOS plays an essential and necessary role in agonist-induced eNOS activation, through enabling its phosphorylation by Akt at serine residue 1177. Serine 178-184 AKT serine/threonine kinase 1 Homo sapiens 171-174 21821012-4 2011 In this study, we show that two PARP1 inhibitors, PJ-34 and 3-AB, attenuate AKT phosphorylation at serine 473 (S473) independent of DNA repair impairment. Serine 99-105 AKT serine/threonine kinase 1 Homo sapiens 76-79 21867682-2 2011 Activation of Akt driven by the growth factor dependent PI3K (phosphatidylinositol-3-OH kinase) is coupled to the plasma membrane translocation and phosphorylation of Akt on two sites by PDK1 (phosphoinositide-dependent protein kinase-1) on Thr-308 and by mTORC2 (mammalian Target of Rapamycin Complex 2) on Ser-473. Serine 308-311 AKT serine/threonine kinase 1 Homo sapiens 14-17 21867682-2 2011 Activation of Akt driven by the growth factor dependent PI3K (phosphatidylinositol-3-OH kinase) is coupled to the plasma membrane translocation and phosphorylation of Akt on two sites by PDK1 (phosphoinositide-dependent protein kinase-1) on Thr-308 and by mTORC2 (mammalian Target of Rapamycin Complex 2) on Ser-473. Serine 308-311 AKT serine/threonine kinase 1 Homo sapiens 167-170 21867682-7 2011 Based on our study we suggest that the mTORC2-dependent phosphorylation of Akt on Ser-473 takes place on the surface of ER. Serine 82-85 AKT serine/threonine kinase 1 Homo sapiens 75-78 21705328-9 2011 One pathway involves PKC-alpha catalyzed phosphorylation of CREB and the other involves a PKC-mediated the PP2A catalyzed dephosphorylation of Akt at Ser(473) which in turn leads to FOXO3a Ser(253) dephosphorylation and its activation. Serine 189-192 AKT serine/threonine kinase 1 Homo sapiens 143-146 21672148-8 2011 In vivo rapamycin treatment induced higher degree of RICTOR and AKT Ser(473) expression directly correlating with long-term rapamycin exposure, FE(PO4) and HOMA index. Serine 68-71 AKT serine/threonine kinase 1 Homo sapiens 64-67 21602475-11 2011 Mimicking AMPK activators in the presence of insulin, rapamycin inhibited p70S6K and reduced IRS-1 phosphorylation on serine, resulting in the overphosphorylation of PKB/Akt and AS160. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 166-173 22140653-3 2011 Inhibition of mTORC2 leads to AKT serine 473 (S473) dephosphorylation and a rapid but transient inhibition of AKT T308 phosphorylation and AKT signaling. Serine 34-40 AKT serine/threonine kinase 1 Homo sapiens 30-33 21592956-4 2011 Both PP242 and Torin1 blocked thrombin and insulin-like growth factor 1-mediated Akt Ser(473) phosphorylation with an IC(50) between 1 and 5 nm, whereas the mTORC1 inhibitor rapamycin had no effect. Serine 85-88 AKT serine/threonine kinase 1 Homo sapiens 81-84 21724622-2 2011 AKT is activated by its phosphorylation at Thr 308 and ser 473 by PDK1 and mTORC2, respectively, in response to trophic stimuli such as insulin and insulin growth factor. Serine 55-58 AKT serine/threonine kinase 1 Homo sapiens 0-3 21592956-0 2011 mTORC2 protein complex-mediated Akt (Protein Kinase B) Serine 473 Phosphorylation is not required for Akt1 activity in human platelets [corrected]. Serine 55-61 AKT serine/threonine kinase 1 Homo sapiens 32-35 21592956-1 2011 Protein kinase B (PKB, Akt) is a Ser/Thr kinase involved in the regulation of cell survival, proliferation, and metabolism and is activated by dual phosphorylation on Thr(308) in the activation loop and Ser(473) in the hydrophobic motif. Serine 33-36 AKT serine/threonine kinase 1 Homo sapiens 23-26 21592956-8 2011 Together, these results demonstrate that mTORC2 is the kinase that phosphorylates Akt Ser(473) in human platelets but that this phosphorylation is dispensable for Thr(308) phosphorylation and Akt1 activity. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 82-85 21732756-2 2011 Akt is recruited to the plasma membrane, a process that facilitates its phosphorylation at threonine 308 and serine (S)473 mediated by the PI3K-dependent kinase 1 and mTORC2 kinase, respectively. Serine 109-115 AKT serine/threonine kinase 1 Homo sapiens 0-3 21505148-8 2011 Muscle lysates were analyzed for AMPK activity and Akt phosphorylation and for TBC1D1 and AS160 phosphorylation on known or putative AMPK and Akt sites as follows: AS160 Ser(711) (AMPK), TBC1D1 Ser(231) (AMPK), TBC1D1 Ser(660) (AMPK), TBC1D1 Ser(700) (AMPK), and TBC1D1 Thr(590) (Akt). Serine 170-173 AKT serine/threonine kinase 1 Homo sapiens 142-145 21505148-8 2011 Muscle lysates were analyzed for AMPK activity and Akt phosphorylation and for TBC1D1 and AS160 phosphorylation on known or putative AMPK and Akt sites as follows: AS160 Ser(711) (AMPK), TBC1D1 Ser(231) (AMPK), TBC1D1 Ser(660) (AMPK), TBC1D1 Ser(700) (AMPK), and TBC1D1 Thr(590) (Akt). Serine 170-173 AKT serine/threonine kinase 1 Homo sapiens 142-145 21750652-6 2011 Full expression of hepsin led to cell death and detachment and was specifically associated with reduced phosphorylation of AKT at Ser(473), which was restored by growth on matrix derived from RWPE1 normal prostatic epithelial cells. Serine 130-133 AKT serine/threonine kinase 1 Homo sapiens 123-126 21750658-5 2011 In this study, we identify merlin serine 10 as a novel substrate for Akt phosphorylation. Serine 34-40 AKT serine/threonine kinase 1 Homo sapiens 69-72 21325073-9 2011 suppressed Akt phosphorylation [at threonine(T)308 and serine(S)473] for up to 36 hours, with cleaved PARP (cPARP) evident up to 18 hours. Serine 55-61 AKT serine/threonine kinase 1 Homo sapiens 11-14 21368105-3 2011 Our data demonstrate that chronic hypoxia promoted mTOR(Ser-2481) phosphorylation, an indicator of mTOR intrinsic catalytic activity, mTORC1-specific S6 and mTORC2-specific Akt (Ser-473) phosphorylation, and proliferation of human and rat PAVSM cells that was inhibited by siRNA mTOR. Serine 178-181 AKT serine/threonine kinase 1 Homo sapiens 173-176 21309701-4 2011 Expressions of PI3-kinase, PKB-kinase, Akt, phosphorylated Akt at Ser(473) (p-Akt), endothelial nitric oxide synthase (eNOS), vascular endothelial-cadherin (VE-cadherin), and actin were examined by Western blot analysis, together with their immunohistochemistry. Serine 66-69 AKT serine/threonine kinase 1 Homo sapiens 59-62 21309701-4 2011 Expressions of PI3-kinase, PKB-kinase, Akt, phosphorylated Akt at Ser(473) (p-Akt), endothelial nitric oxide synthase (eNOS), vascular endothelial-cadherin (VE-cadherin), and actin were examined by Western blot analysis, together with their immunohistochemistry. Serine 66-69 AKT serine/threonine kinase 1 Homo sapiens 59-62 21505451-2 2011 Akt activation involves the phosphorylation of two residues: threonine 308 (Thr308) in the activation loop and serine 473 (Ser473) in the C-terminal hydrophobic motif. Serine 111-117 AKT serine/threonine kinase 1 Homo sapiens 0-3 21397040-10 2011 Incubation of HUVECs with alogliptin resulted in eNOS and Akt phosphorylation (Ser(1177) and Ser(473) respectively) paralleled by a rapid increase in nitric oxide. Serine 79-82 AKT serine/threonine kinase 1 Homo sapiens 58-61 21487020-7 2011 Moreover, nuclear translocation of EGFR requires phosphorylation at Ser-229 by Akt. Serine 68-71 AKT serine/threonine kinase 1 Homo sapiens 79-82 21325637-3 2011 To date, the roles of PDGF-induced key proliferative signaling components including Akt, p70S6kinase, and ERK1/2 on the serine phosphorylation/expression of IRS-1 and IRS-2 isoforms remain unclear in VSMCs. Serine 120-126 AKT serine/threonine kinase 1 Homo sapiens 84-87 21325637-7 2011 Notably, phosphoinositide 3-kinase (PI 3-kinase) inhibitor (PI-103) and mammalian target of rapamycin inhibitor (rapamycin), which abolished PDGF-induced Akt and p70S6kinase phosphorylation, respectively, blocked PDGF-induced IRS-1 serine phosphorylation and IRS-2 downregulation. Serine 232-238 AKT serine/threonine kinase 1 Homo sapiens 154-157 21559284-8 2011 Conversely, the OGTT induced acute phosphorylation of the insulin signaling proteins Akt (ser(473) and thr(308)), and AS160.The combination of OGTT and GH suppressed Akt activation, whereas the downstream expression of AS160 was amplified by GH. Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 85-88 21413766-7 2011 Upstream of the EphA2 phosphorylation, activation of Akt1 on Ser 129 was also revealed as part of the ephrin B3-mediated signaling pathway. Serine 61-64 AKT serine/threonine kinase 1 Homo sapiens 53-57 21454539-0 2011 Phosphomimetic substitution of heterogeneous nuclear ribonucleoprotein A1 at serine 199 abolishes AKT-dependent internal ribosome entry site-transacting factor (ITAF) function via effects on strand annealing and results in mammalian target of rapamycin complex 1 (mTORC1) inhibitor sensitivity. Serine 77-83 AKT serine/threonine kinase 1 Homo sapiens 98-101 21454539-5 2011 AKT phosphorylation of hnRNP A1 at serine 199 has been demonstrated to inhibit IRES-mediated translation initiation. Serine 35-41 AKT serine/threonine kinase 1 Homo sapiens 0-3 21454539-8 2011 We also demonstrate that activated AKT is strongly correlated with elevated Ser(P)(199)-hnRNP A1 levels in a panel of 22 glioblastomas. Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 35-38 21454539-9 2011 These data demonstrate that the phosphorylation status of hnRNP A1 serine 199 regulates the AKT-dependent sensitivity of cells to rapamycin and functionally links IRES-transacting factor annealing activity to cellular responses to mTOR complex 1 inhibition. Serine 67-73 AKT serine/threonine kinase 1 Homo sapiens 92-95 21378028-4 2011 Immunostains of human airway sections revealed concordant activation of Akt (reflected in Ser(473) phosphorylation) and of its downstream effector p70(S6Kinase) (reflected in Thr(389) phosphorylation) within airway muscle bundles, but there was no phosphorylation of the alternative Akt downstream target glycogen synthase kinase (GSK) 3beta. Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 72-75 21321187-5 2011 This structural modification increased Akt1 phosphorylation at Ser(473) and Thr(308). Serine 63-66 AKT serine/threonine kinase 1 Homo sapiens 39-43 21360572-2 2011 Serine 9 phosphorylation of GSK3beta (pGSK3betaSer9), usually promoted by activation of the PI3K/Akt survival pathway, triggers GSK3beta inhibition. Serine 0-6 AKT serine/threonine kinase 1 Homo sapiens 97-100 21343617-5 2011 We found that during cellular stress, GSK-3beta phosphorylated the mTORC2 component rictor at serine-1235, a modification that interfered with the binding of Akt to mTORC2. Serine 94-100 AKT serine/threonine kinase 1 Homo sapiens 158-161 21308747-7 2011 Moreover, SPH-induced dephosphorylation of Akt at Ser(473) subsequently leads to the activation of GSK-3beta, caspase 3, PARP cleavage, and ultimately apoptosis. Serine 50-53 AKT serine/threonine kinase 1 Homo sapiens 43-46 21177249-5 2011 Phosphorylation of Akt at Ser-473 was enhanced by overexpression of PINK1, and the Akt activation was crucial for protection of SH-SY5Y cells from various cytotoxic agents, including oxidative stress. Serine 26-29 AKT serine/threonine kinase 1 Homo sapiens 19-22 21209368-0 2011 Akt directly regulates focal adhesion kinase through association and serine phosphorylation: implication for pressure-induced colon cancer metastasis. Serine 69-75 AKT serine/threonine kinase 1 Homo sapiens 0-3 21209368-9 2011 In addition, our results demonstrated that Akt phosphorylated FAK at three novel serine phosphorylation sites, which were also not required for FAK-Akt binding. Serine 81-87 AKT serine/threonine kinase 1 Homo sapiens 43-46 21343617-6 2011 The inhibitory effect of GSK-3beta on mTORC2-Akt signaling and cell proliferation was eliminated by blocking phosphorylation of rictor at serine-1235. Serine 138-144 AKT serine/threonine kinase 1 Homo sapiens 45-48 21321111-3 2011 Although it is well documented that the mammalian target of rapamycin complex 2 (mTORC2)-dependent phosphorylation of the Akt hydrophobic motif (Ser-473 in Akt1) is essential for full Akt activation, it remains unclear whether this phosphorylation has additional roles in regulating Akt activity. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 122-125 21321111-3 2011 Although it is well documented that the mammalian target of rapamycin complex 2 (mTORC2)-dependent phosphorylation of the Akt hydrophobic motif (Ser-473 in Akt1) is essential for full Akt activation, it remains unclear whether this phosphorylation has additional roles in regulating Akt activity. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 156-160 21321111-3 2011 Although it is well documented that the mammalian target of rapamycin complex 2 (mTORC2)-dependent phosphorylation of the Akt hydrophobic motif (Ser-473 in Akt1) is essential for full Akt activation, it remains unclear whether this phosphorylation has additional roles in regulating Akt activity. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 156-159 21321111-3 2011 Although it is well documented that the mammalian target of rapamycin complex 2 (mTORC2)-dependent phosphorylation of the Akt hydrophobic motif (Ser-473 in Akt1) is essential for full Akt activation, it remains unclear whether this phosphorylation has additional roles in regulating Akt activity. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 156-159 21321111-4 2011 In this study, we found that abolishing Akt Ser-473 phosphorylation stabilizes Akt following agonist stimulation. Serine 44-47 AKT serine/threonine kinase 1 Homo sapiens 40-43 21321111-4 2011 In this study, we found that abolishing Akt Ser-473 phosphorylation stabilizes Akt following agonist stimulation. Serine 44-47 AKT serine/threonine kinase 1 Homo sapiens 79-82 21321111-5 2011 The Akt Ser-473 phosphorylation promotes a Lys-48-linked polyubiquitination of Akt, resulting in its rapid proteasomal degradation. Serine 8-11 AKT serine/threonine kinase 1 Homo sapiens 4-7 21321111-5 2011 The Akt Ser-473 phosphorylation promotes a Lys-48-linked polyubiquitination of Akt, resulting in its rapid proteasomal degradation. Serine 8-11 AKT serine/threonine kinase 1 Homo sapiens 79-82 21321111-8 2011 Taken together, this study reveals that the Akt Ser-473 phosphorylation-dependent ubiquitination and degradation is an important negative feedback regulation that specifically terminates Akt activation. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 44-47 21321111-8 2011 Taken together, this study reveals that the Akt Ser-473 phosphorylation-dependent ubiquitination and degradation is an important negative feedback regulation that specifically terminates Akt activation. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 187-190 21444773-6 2011 Consistent with the hypothesis, PRMT1-mediated methylation of these two arginine residues inhibits Akt-mediated phosphorylation of BAD at Ser-99 in vitro and in vivo. Serine 138-141 AKT serine/threonine kinase 1 Homo sapiens 99-102 21310961-3 2011 Although phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P(3)) is known to regulate the phosphorylation of AKT Ser(473), the hydrophobic motif (HM) site, by mTORC2, it is not clear whether PtdIns(3,4,5)P(3) can directly regulate mTORC2 kinase activity. Serine 118-121 AKT serine/threonine kinase 1 Homo sapiens 114-117 21392397-2 2011 Phosphorylation of YB-1 at serine residue 102 (S102) in response to growth factors is required for its transcriptional activity and is thought to be regulated by cytoplasmic signaling phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathways. Serine 27-33 AKT serine/threonine kinase 1 Homo sapiens 221-224 21183727-7 2011 Western blot analysis revealed that resistin treatment was associated with enhanced phosphorylation of Akt, at both the serine-473 (+ 51.9%, P = .01) and threonine-308 (+107%, P < .01) phosphorylation sites, although not to the extent seen with ischemic preconditioning (+132.5%, P = .002 and +389.1%, P < .01, respectively). Serine 120-126 AKT serine/threonine kinase 1 Homo sapiens 103-106 21131356-6 2011 Whereas both phosphorylations were dependent on the presence of mTOR, only Akt phosphorylation at Ser-473 was dependent on the presence of rictor, a specific component of mTORC2. Serine 98-101 AKT serine/threonine kinase 1 Homo sapiens 75-78 22060274-8 2011 Pathway analysis was performed by pharmacological inhibition of phosphorylation of AKT (pAKT-Ser 473) or modulation of TWIST1 expression. Serine 93-96 AKT serine/threonine kinase 1 Homo sapiens 83-86 21097843-7 2011 The expression of EEA1 is required for phosphorylation of Akt at both Thr-308 and Ser-473 as well as for phosphorylation of its downstream targets mTOR and S6 kinase, but not for Erk1/2 activation. Serine 82-85 AKT serine/threonine kinase 1 Homo sapiens 58-61 21110830-4 2011 Analysis of all Akt kinases reveals conserved homology for an N-terminal regulatory domain, which contains a pleckstrin-homology (PH) domain for cellular translocation, a kinase domain with serine/threonine specificity, and a C-terminal extension domain. Serine 190-196 AKT serine/threonine kinase 1 Homo sapiens 16-19 20688159-3 2011 Overexpression of Akt1 down-regulated ERK activity inhibiting Ser 259 phosphorylation of c-Raf and subsequent downstream signaling. Serine 62-65 AKT serine/threonine kinase 1 Homo sapiens 18-22 21035837-7 2011 In addition, thioridazine was found to inhibit p-Akt (Ser 473) in a dose-dependent manner. Serine 54-57 AKT serine/threonine kinase 1 Homo sapiens 49-52 21998731-8 2011 HF-mediated apoptosis was associated with dephosphorylation of active Akt1 (at Ser(473)) and Akt1 substrate Bad (at Ser(136)) which activates Bad pro-apoptotic function. Serine 79-82 AKT serine/threonine kinase 1 Homo sapiens 70-74 21998731-8 2011 HF-mediated apoptosis was associated with dephosphorylation of active Akt1 (at Ser(473)) and Akt1 substrate Bad (at Ser(136)) which activates Bad pro-apoptotic function. Serine 116-119 AKT serine/threonine kinase 1 Homo sapiens 70-74 21998731-8 2011 HF-mediated apoptosis was associated with dephosphorylation of active Akt1 (at Ser(473)) and Akt1 substrate Bad (at Ser(136)) which activates Bad pro-apoptotic function. Serine 116-119 AKT serine/threonine kinase 1 Homo sapiens 93-97 20980511-3 2011 Akt phosphorylation at both threonine 308 (Thr308) and serine 473 (Ser473) was inhibited in cells infected with VSV. Serine 55-61 AKT serine/threonine kinase 1 Homo sapiens 0-3 21045158-9 2010 Finally, Akt was found to mediate exclusion of the exon 3,4,5,6 cassette of Casp9 via the phosphorylation state of the RNA splicing factor SRp30a via serines 199, 201, 227, and 234. Serine 150-157 AKT serine/threonine kinase 1 Homo sapiens 9-12 21034728-1 2010 Insulin-like growth factor-I and insulin induce the production of phospho-Ser-166 MDM2, a target of Akt, and influence the formation of the MDM2 complex. Serine 74-77 AKT serine/threonine kinase 1 Homo sapiens 100-103 20826143-7 2010 PMA inhibited insulin-stimulated phosphorylation of Tyr in insulin receptor beta subunit and Ser/Thr in Akt. Serine 93-96 AKT serine/threonine kinase 1 Homo sapiens 104-107 21142450-6 2010 Furthermore, epithelial 14.7K expression inhibited serine phosphorylation of Akt, GSK-3beta, and the p65 subunit of NF-kappaB, as well as recruitment of NF-kappaB for DNA binding in vivo. Serine 51-57 AKT serine/threonine kinase 1 Homo sapiens 77-80 20729916-5 2010 Inhibiting AKT decreased serine-127 phosphorylation and enhanced nuclear translocation of YAP. Serine 25-31 AKT serine/threonine kinase 1 Homo sapiens 11-14 20686120-4 2010 Although comparable to rapamycin against phosphorylation of the TORC1 substrates p70S6kinase and 4E-BP-1, pp242 could also inhibit phosphorylation of AKT on serine 473, a TORC2 substrate, while rapamycin was ineffective. Serine 157-163 AKT serine/threonine kinase 1 Homo sapiens 150-153 20686120-8 2010 TORC2 activation was frequently identified in primary specimens by immunostaining for AKT phosphorylation on serine 473. Serine 109-115 AKT serine/threonine kinase 1 Homo sapiens 86-89 20601126-4 2010 We found that Ang II significantly inhibited insulin-induced phosphorylation of tyrosine 608 of IRS-1 and serine 473 of Akt, a downstream member of anti-mitogenic pathway of insulin. Serine 106-112 AKT serine/threonine kinase 1 Homo sapiens 120-123 20597130-0 2010 Insulin-like growth factor-1 receptor and phosphorylated AKT-serine 473 expression in 132 resected thymomas and thymic carcinomas. Serine 61-67 AKT serine/threonine kinase 1 Homo sapiens 57-60 20597130-4 2010 METHODS: The authors investigated the expression of IGF-1R and phosphorylated AKT serine 473 (p-AKT) by using immunohistochemistry and examined the clinicopathologic correlations in a retrospective, single-institution surgical series of 132 patients with thymic epithelial malignancies. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 78-81 20696212-3 2010 In this study, we demonstrated that insulin inhibited PS1 phosphorylation at serine residues (serine 353, 357) via phosphatidylinositol 3-kinase (PI3K)/Akt signal pathway and strengthened the trimeric complex of PS1/N-cadherin/beta-catenin, consequently relocalizing PS1 to the cell surface. Serine 77-83 AKT serine/threonine kinase 1 Homo sapiens 152-155 20696212-3 2010 In this study, we demonstrated that insulin inhibited PS1 phosphorylation at serine residues (serine 353, 357) via phosphatidylinositol 3-kinase (PI3K)/Akt signal pathway and strengthened the trimeric complex of PS1/N-cadherin/beta-catenin, consequently relocalizing PS1 to the cell surface. Serine 94-100 AKT serine/threonine kinase 1 Homo sapiens 152-155 20682768-3 2010 AKT directly binds to and phosphorylates CLK2 on serine 34 and threonine 127, in vitro and in vivo. Serine 49-55 AKT serine/threonine kinase 1 Homo sapiens 0-3 20648624-5 2010 In this study we show that PED coadiuvates Rac1 activation by regulating AKT mediated Rac1-Ser(71) phosphorylation. Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 73-76 20451318-5 2010 SFN also suppressed the phosphorylation of Akt (Ser-473), thereby inhibiting hTERT phosphorylation and this effect was reversed by pretreatment with NAC. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 43-46 20691662-0 2010 TCR-induced Akt serine 473 phosphorylation is regulated by protein kinase C-alpha. Serine 16-22 AKT serine/threonine kinase 1 Homo sapiens 12-15 20691662-2 2010 Phosphorylation at Ser(473) in the hydrophobic motif, along with Thr(308) in its activation loop, is considered necessary for Akt function. Serine 19-22 AKT serine/threonine kinase 1 Homo sapiens 126-129 20691662-3 2010 It is widely accepted that phosphoinositide-dependent kinase 1 (PDK-1) phosphorylates Akt at Thr(308), but the kinase(s) responsible for phosphorylating Akt at Ser(473) (PDK-2) remains elusive. Serine 160-163 AKT serine/threonine kinase 1 Homo sapiens 153-156 20691662-6 2010 In this study, we found that conventional PKC positively regulated TCR-induced Akt Ser(473) phosphorylation. Serine 83-86 AKT serine/threonine kinase 1 Homo sapiens 79-82 20691662-7 2010 PKC-alpha purified from T cells can phosphorylate Akt at Ser(473) in vitro upon TCR stimulation. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 50-53 21368869-5 2010 We show that Akt can phosphorylate the serine 134 residue of CSDA but, downstream of Bcr-Abl activity, this modification is mediated through the activation of MEK/p90 ribosomal S6 kinase (RSK) signaling. Serine 39-45 AKT serine/threonine kinase 1 Homo sapiens 13-16 20844753-2 2010 We recently illustrated by an in vitro kinase assay that a novel peptide to YB-1 was highly phosphorylated by the serine/threonine p90 S6 kinases RSK-1 and RSK-2, and to a lesser degree PKCalpha and AKT. Serine 114-120 AKT serine/threonine kinase 1 Homo sapiens 199-202 20186153-2 2010 Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases to prime its activation; then, phosphoinositide-dependent kinase 1 phosphorylates AKT at Thr-308 to expose the Ser-473 residue; and finally, AKT is phosphorylated at Ser-473 by several kinases (PKD2 and others) to achieve its full activation. Serine 269-272 AKT serine/threonine kinase 1 Homo sapiens 33-36 20186153-2 2010 Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases to prime its activation; then, phosphoinositide-dependent kinase 1 phosphorylates AKT at Thr-308 to expose the Ser-473 residue; and finally, AKT is phosphorylated at Ser-473 by several kinases (PKD2 and others) to achieve its full activation. Serine 269-272 AKT serine/threonine kinase 1 Homo sapiens 110-113 20186153-2 2010 Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases to prime its activation; then, phosphoinositide-dependent kinase 1 phosphorylates AKT at Thr-308 to expose the Ser-473 residue; and finally, AKT is phosphorylated at Ser-473 by several kinases (PKD2 and others) to achieve its full activation. Serine 269-272 AKT serine/threonine kinase 1 Homo sapiens 110-113 20186153-2 2010 Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases to prime its activation; then, phosphoinositide-dependent kinase 1 phosphorylates AKT at Thr-308 to expose the Ser-473 residue; and finally, AKT is phosphorylated at Ser-473 by several kinases (PKD2 and others) to achieve its full activation. Serine 269-272 AKT serine/threonine kinase 1 Homo sapiens 110-113 20186153-2 2010 Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases to prime its activation; then, phosphoinositide-dependent kinase 1 phosphorylates AKT at Thr-308 to expose the Ser-473 residue; and finally, AKT is phosphorylated at Ser-473 by several kinases (PKD2 and others) to achieve its full activation. Serine 324-327 AKT serine/threonine kinase 1 Homo sapiens 33-36 20186153-2 2010 Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases to prime its activation; then, phosphoinositide-dependent kinase 1 phosphorylates AKT at Thr-308 to expose the Ser-473 residue; and finally, AKT is phosphorylated at Ser-473 by several kinases (PKD2 and others) to achieve its full activation. Serine 324-327 AKT serine/threonine kinase 1 Homo sapiens 110-113 20186153-2 2010 Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases to prime its activation; then, phosphoinositide-dependent kinase 1 phosphorylates AKT at Thr-308 to expose the Ser-473 residue; and finally, AKT is phosphorylated at Ser-473 by several kinases (PKD2 and others) to achieve its full activation. Serine 324-327 AKT serine/threonine kinase 1 Homo sapiens 110-113 20186153-2 2010 Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases to prime its activation; then, phosphoinositide-dependent kinase 1 phosphorylates AKT at Thr-308 to expose the Ser-473 residue; and finally, AKT is phosphorylated at Ser-473 by several kinases (PKD2 and others) to achieve its full activation. Serine 324-327 AKT serine/threonine kinase 1 Homo sapiens 110-113 20186153-3 2010 For its inactivation, the PH-domain containing phosphatases dephosphorylate AKT at Ser-473, and protein serine/threonine phosphatase-2A (PP-2A) dephosphorylates it at Thr-308. Serine 83-86 AKT serine/threonine kinase 1 Homo sapiens 76-79 20516073-6 2010 PKA and PI3-K/Akt transactivate the NF-kappaB p65 subunit via phosphorylation at Ser-276 and Ser-536, respectively. Serine 81-84 AKT serine/threonine kinase 1 Homo sapiens 14-17 20736484-2 2010 The Akt, RSK, and p70 S6 family of protein kinases transmits signals by phosphorylating substrates on an RxRxxS/T motif (R, arginine; S, serine; T, threonine; and x, any amino acid). Serine 137-143 AKT serine/threonine kinase 1 Homo sapiens 4-7 20167420-4 2010 Further studies reveal that IBC significantly abates Akt phosphorylation at Ser-473 and Akt kinase activity in cells, which subsequently leads to inhibition of Akt downstream substrates and evokes significant levels of apoptosis associated with mitochondria pathway. Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 53-56 20516073-6 2010 PKA and PI3-K/Akt transactivate the NF-kappaB p65 subunit via phosphorylation at Ser-276 and Ser-536, respectively. Serine 93-96 AKT serine/threonine kinase 1 Homo sapiens 14-17 20555032-8 2010 IGF-I-stimulated phosphorylation of AKT on serine(473) but not threonine(308) was decreased in omental cells, and activation of downstream targets, including S6Kinase, glycogen synthase kinase-3, and Forkhead box O1 was also impaired. Serine 43-49 AKT serine/threonine kinase 1 Homo sapiens 36-39 20555032-10 2010 Over-expression of IRS-1 by lentivirus in omental preadipocytes increased IGF-I-stimulated AKT-serine(473) phosphorylation. Serine 95-101 AKT serine/threonine kinase 1 Homo sapiens 91-94 20530982-7 2010 Akt phosphorylation on Ser-473, which is catalyzed by mTOR and required for Akt activation, increased with increasing amounts of IQGAP1, and decreased with IQGAP1 mutation. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 0-3 20547325-3 2010 HRAP enhanced the inhibition of phosphorylation of serine 473 in Akt and Ser380/Thy382/The383 in PTEN. Serine 51-57 AKT serine/threonine kinase 1 Homo sapiens 65-68 20530982-7 2010 Akt phosphorylation on Ser-473, which is catalyzed by mTOR and required for Akt activation, increased with increasing amounts of IQGAP1, and decreased with IQGAP1 mutation. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 76-79 20427478-6 2010 More importantly, upregulation of p70S6K signaling impaired insulin-stimulated phosphorylation of Akt Ser(473) and p70S6K in IR(+/+) and Rec B but not in Rec A cell lines. Serine 102-105 AKT serine/threonine kinase 1 Homo sapiens 98-101 20362557-7 2010 GlbA treatment also promoted the activation of Akt/PKB via phosphorylation at residue Ser(473) and induced autophagy as judged by the presence of the lipidated form of microtubule-associated protein 1 light chain 3 (LC3) and autophagosomes. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 47-54 20515848-3 2010 The Akt / protein kinase B (PKB) is a family of serine / threonine protein kinases, which play prominent roles in a diverse number of processes including cell survival, cell growth, gene expression, apoptosis, protein synthesis, energy metabolism and oncogenesis. Serine 48-54 AKT serine/threonine kinase 1 Homo sapiens 4-7 20527022-3 2010 In this process, we also found that bFGF inhibited Akt phosphorylation at serine 473 and induced a rapid loss of phosphorylation of focal adhesion kinase (FAK) at tyrosine 397 in pretreated GF-1 cells, an event that coincided with the dissociation of phosphorylated FAK from the focal adhesions. Serine 74-80 AKT serine/threonine kinase 1 Homo sapiens 51-54 20513428-6 2010 However, if Akt is inactive, GSK3beta phosphorylates p52 at Ser 222. Serine 60-63 AKT serine/threonine kinase 1 Homo sapiens 12-15 20501647-3 2010 The PI3K-dependent signaling kinase complex defined as mammalian target of rapamycin complex 2 (mTORC2) functions as a regulatory Ser-473 kinase of Akt. Serine 130-133 AKT serine/threonine kinase 1 Homo sapiens 148-151 21234858-8 2010 Western blotting studies revealed that AGEs decreased NOS-3 phosphorylation on serine-1177, increased NOS-3 O-glycosylation, and decreased serine phosphorylation of protein kinase Akt; all of these changes were abrogated by pyridoxine. Serine 139-145 AKT serine/threonine kinase 1 Homo sapiens 180-183 20357092-4 2010 Phosphorylation of FoxO1 at Ser(256), a downstream target for Akt, was inhibited in hepatocytes infected with HCV or expressing the core protein or full-length (FL) genome of HCV. Serine 28-31 AKT serine/threonine kinase 1 Homo sapiens 62-65 20299462-4 2010 We hypothesized that Ang II stimulates TAL NO production via AT(2)-mediated Akt1 activation, which phosphorylates NOS3 at serine 1177. Serine 122-128 AKT serine/threonine kinase 1 Homo sapiens 76-80 20299462-18 2010 We concluded that Ang II enhances TAL NO production via activation of AT(2) and Akt1-dependent phosphorylation of NOS3 at serines 1177 and 633. Serine 122-129 AKT serine/threonine kinase 1 Homo sapiens 80-84 20364113-7 2010 mTORC2 activates AKT directly by phosphorylating Serine 473. pAKT(S473) phosphorylates TSC2 (tuberin) and inactivates it, preventing its association with TSC1 (hamartin) and the inhibition of Rheb, an activator of mTOR. Serine 49-55 AKT serine/threonine kinase 1 Homo sapiens 17-20 20383171-13 2010 AKT ser(473) phosphorylation was also enhanced significantly in the presence of berberine. Serine 4-7 AKT serine/threonine kinase 1 Homo sapiens 0-3 20384568-4 2010 Ang II caused significant (p < 0.05) inhibition of insulin-stimulated glucose transport (39%) and decreased phosphorylation of Akt Ser(473) (37%) and glycogen synthase kinase-3beta Ser(9) (42%) without affecting phosphorylation of IRS-1 Ser(307) or p38 MAPK. Serine 134-137 AKT serine/threonine kinase 1 Homo sapiens 130-133 20384568-4 2010 Ang II caused significant (p < 0.05) inhibition of insulin-stimulated glucose transport (39%) and decreased phosphorylation of Akt Ser(473) (37%) and glycogen synthase kinase-3beta Ser(9) (42%) without affecting phosphorylation of IRS-1 Ser(307) or p38 MAPK. Serine 184-187 AKT serine/threonine kinase 1 Homo sapiens 130-133 20384568-4 2010 Ang II caused significant (p < 0.05) inhibition of insulin-stimulated glucose transport (39%) and decreased phosphorylation of Akt Ser(473) (37%) and glycogen synthase kinase-3beta Ser(9) (42%) without affecting phosphorylation of IRS-1 Ser(307) or p38 MAPK. Serine 184-187 AKT serine/threonine kinase 1 Homo sapiens 130-133 20395207-10 2010 The relevance of our studies to human breast cancer is highlighted by the finding that low CCN6 protein levels are associated with upregulated expression of phospho-Akt-1 (Ser(473)) in 21% of invasive breast carcinomas. Serine 172-175 AKT serine/threonine kinase 1 Homo sapiens 165-170 20411591-9 2010 Bradykinin stimulation also increased the phosphorylation of the p85 subunit of PI-3 kinase and serine 473 of AKT. Serine 96-102 AKT serine/threonine kinase 1 Homo sapiens 110-113 20224869-5 2010 The polyphenol-induced NO formation is due to the redox-sensitive activation of the phosphatidylinositol3-kinase/Akt pathway leading to endothelial NO synthase (eNOS) activation subsequent to its phosphorylation on Ser 1177. Serine 215-218 AKT serine/threonine kinase 1 Homo sapiens 113-116 20348139-13 2010 CONCLUSIONS: Propofol induces the Ser(1177) phosphorylation-dependent eNOS activation through the drug-stimulated translocation of PKC isoforms to distinct intracellular sites in HUVECs, which is independent of PI3K/Akt-independent pathway. Serine 34-37 AKT serine/threonine kinase 1 Homo sapiens 216-219 20179300-4 2010 Activation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin axis normally results in serine phosphorylation and subsequent downregulation of these adaptor proteins. Serine 103-109 AKT serine/threonine kinase 1 Homo sapiens 44-47 20383279-5 2010 Tyrosine nitration of ERK1/2 was required for their phosphorylation on Thr and Tyr and their subsequent activation, whereas it completely inhibited Akt phosphorylation on Ser(473) and Thr(308) as well as its activity. Serine 171-174 AKT serine/threonine kinase 1 Homo sapiens 148-151 20068047-6 2010 Notably, the kinase activity and phosphorylation (e.g. Thr(P)(308) and Ser(P)(473)) levels of constitutively active Akt, including a naturally occurring mutant AKT1-E17K, were inhibited by API-1. Serine 71-74 AKT serine/threonine kinase 1 Homo sapiens 116-119 20054000-6 2010 Of the targets in this pathway, HQ directly targeted and bound to the sulfhydryl group of Cys-310 of Akt and sequentially interrupted the phosphorylation of both Thr-308 and Ser-473 by mediation of beta-mercaptoethanol, according to the liquid chromatography/mass spectroscopy analysis of the interaction of HQ with an Akt-derived peptide. Serine 174-177 AKT serine/threonine kinase 1 Homo sapiens 101-104 20054000-6 2010 Of the targets in this pathway, HQ directly targeted and bound to the sulfhydryl group of Cys-310 of Akt and sequentially interrupted the phosphorylation of both Thr-308 and Ser-473 by mediation of beta-mercaptoethanol, according to the liquid chromatography/mass spectroscopy analysis of the interaction of HQ with an Akt-derived peptide. Serine 174-177 AKT serine/threonine kinase 1 Homo sapiens 319-322 19757038-7 2010 In addition, we found suppression of SSeCKS expression promoted Akt serine 473 phosphorylation in cAMP-treated Schwann cells. Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 64-67 19687739-9 2010 Akt was not phosphorylated in response to propofol (50 micromol l(-1)) at serine(473). Serine 74-80 AKT serine/threonine kinase 1 Homo sapiens 0-3 20145118-6 2010 Furthermore, MLK3 inhibition by E(2) involved phosphorylation of MLK3 Ser(674) by AKT, attenuating the proapoptotic function of MLK3. Serine 70-73 AKT serine/threonine kinase 1 Homo sapiens 82-85 20014066-7 2010 The inhibition of mTORC2 results in the de-phosphorylation of Akt at Ser(473) in the hydrophobic motif of C-terminal tail required for Akt full activation and precludes Akt re-phosphorylation in response to IM. Serine 69-72 AKT serine/threonine kinase 1 Homo sapiens 62-65 20014066-7 2010 The inhibition of mTORC2 results in the de-phosphorylation of Akt at Ser(473) in the hydrophobic motif of C-terminal tail required for Akt full activation and precludes Akt re-phosphorylation in response to IM. Serine 69-72 AKT serine/threonine kinase 1 Homo sapiens 135-138 20014066-7 2010 The inhibition of mTORC2 results in the de-phosphorylation of Akt at Ser(473) in the hydrophobic motif of C-terminal tail required for Akt full activation and precludes Akt re-phosphorylation in response to IM. Serine 69-72 AKT serine/threonine kinase 1 Homo sapiens 135-138 19915805-2 2010 Insulin signaling through Akt kinase has been demonstrated to phosphorylate PGC-1alpha at serine 571 and downregulate its activity in the liver. Serine 90-96 AKT serine/threonine kinase 1 Homo sapiens 26-29 19925457-7 2010 We propose that the rapid PKB-mediated stimulation of phosphorylation of Ser-615 contributes to insulin-stimulated NO synthesis. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 26-29 20045673-6 2010 The majority of OSCC cell lines showed an activation of PI3K/Akt signaling, and enforced expression of NDRG2 in HSC-3 cells decreased the level of phosphorylated Akt at Serine 473 (p-Akt). Serine 169-175 AKT serine/threonine kinase 1 Homo sapiens 162-165 19925457-5 2010 Insulin-stimulated Ser-615 phosphorylation was abrogated by incubation with the PI3K (phosphoinositide 3-kinase) inhibitor wortmannin, infection with adenoviruses expressing a dominant-negative mutant PKB/Akt or pre-incubation with TNFalpha (tumour necrosis factor alpha), but was unaffected by high culture glucose concentrations. Serine 19-22 AKT serine/threonine kinase 1 Homo sapiens 201-204 19925457-5 2010 Insulin-stimulated Ser-615 phosphorylation was abrogated by incubation with the PI3K (phosphoinositide 3-kinase) inhibitor wortmannin, infection with adenoviruses expressing a dominant-negative mutant PKB/Akt or pre-incubation with TNFalpha (tumour necrosis factor alpha), but was unaffected by high culture glucose concentrations. Serine 19-22 AKT serine/threonine kinase 1 Homo sapiens 205-208 20045673-6 2010 The majority of OSCC cell lines showed an activation of PI3K/Akt signaling, and enforced expression of NDRG2 in HSC-3 cells decreased the level of phosphorylated Akt at Serine 473 (p-Akt). Serine 169-175 AKT serine/threonine kinase 1 Homo sapiens 162-165 20068168-4 2010 Here, we investigated this model using the somatostatin analogue octreotide as a tool to decrease levels of activated Ser(473)-phosphorylated Akt (pAkt-Ser(473)) in pituitary tumor cells that express somatostatin receptors. Serine 118-121 AKT serine/threonine kinase 1 Homo sapiens 142-145 20068168-4 2010 Here, we investigated this model using the somatostatin analogue octreotide as a tool to decrease levels of activated Ser(473)-phosphorylated Akt (pAkt-Ser(473)) in pituitary tumor cells that express somatostatin receptors. Serine 152-155 AKT serine/threonine kinase 1 Homo sapiens 142-145 20217555-4 2010 Here, we demonstrate that PI3K inhibition affects FLJ10540-mediated cell migration and invasion and further, that FLJ10540 knockdown ablates AKT-Ser(473) phosphorylation. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 141-144 21192818-10 2010 Stimulating cells with chemerin(21-157) resulted in phosphorylation of p44/p42 MAPKs (ERK 1/2) and Akt (Ser 473). Serine 104-107 AKT serine/threonine kinase 1 Homo sapiens 99-102 21326806-6 2010 Moreover, its putative downstream effector, Akt phosphorylated on serine 473 represents a signal transduction pathway for tumor survival. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 44-47 20042122-3 2009 In this paper, we employed an RNA interference library targeting all human kinases to screen for kinases involved in the regulation of Akt activation, in particular serine 473 phosphorylation. Serine 165-171 AKT serine/threonine kinase 1 Homo sapiens 135-138 19672862-5 2009 Despite the absence of functional phosphatase and tensin homolog (PTEN) protein in Ishikawa cells, PKCalpha knockdown reduced Akt phosphorylation at serine 473 and concomitantly inhibited phosphorylation of the Akt target, glycogen synthase kinase-3beta (GSK-3beta). Serine 149-155 AKT serine/threonine kinase 1 Homo sapiens 126-129 19888469-8 2009 Consistent with this, we found that ghrelin promoted the PI3-K/Akt pathway in a Galphaq-dependent manner, resulting in Akt recruitment to PPARgamma, enhanced PPARgamma phosphorylation and activation independently of Ser-84, and increased expression of LXRalpha and ABCA1/G1. Serine 216-219 AKT serine/threonine kinase 1 Homo sapiens 63-66 20015441-4 2009 After 1- or 2-day culture, Astragalus injection, astragaloside IV, formononetin and astragaloside IV plus formononetin decreased the expressions of p-Akt (Thr 308) and p-Akt (Ser 473) in MDA-MB-468 cells. Serine 175-178 AKT serine/threonine kinase 1 Homo sapiens 170-173 19526459-6 2009 Mutation of the phosphorylation site from serine to alanine rescues the DNA-binding ability in the presence of activated AKT. Serine 42-48 AKT serine/threonine kinase 1 Homo sapiens 121-124 19724902-7 2009 Interestingly, knockdown of either of RQCD1, GIGYF1 or GIGYF2 resulted in significant reduction of the phosphorylation of Akt at Ser 473 in breast cancer cell lines. Serine 129-132 AKT serine/threonine kinase 1 Homo sapiens 122-125 19701246-4 2009 Akt activation, as determined by serine-473 phosphorylation, was inhibited by ACS-1 in cells stimulated with either EGF or fibronectin. Serine 33-39 AKT serine/threonine kinase 1 Homo sapiens 0-3 19863783-7 2009 Interestingly, mTORC2 has been shown to contain the kinase responsible for the phosphorylation of Akt at Serine 473. Serine 105-111 AKT serine/threonine kinase 1 Homo sapiens 98-101 19703998-0 2009 Mutation of the Rb1 pathway leads to overexpression of mTor, constitutive phosphorylation of Akt on serine 473, resistance to anoikis, and a block in c-Raf activation. Serine 100-106 AKT serine/threonine kinase 1 Homo sapiens 93-96 19703998-3 2009 Phosphorylation on serine 473 (Akt-473) is catalyzed by mTor in a second complex (TORC2), and Akt-473 phosphorylates Foxo3a to inhibit apoptosis. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 31-34 19703998-3 2009 Phosphorylation on serine 473 (Akt-473) is catalyzed by mTor in a second complex (TORC2), and Akt-473 phosphorylates Foxo3a to inhibit apoptosis. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 94-97 19861406-6 2009 However, small interfering RNA (siRNA)-mediated depletion of either Akt1 or Akt2 showed that BAD serine 136 phosphorylation is dependent specifically on Akt2 function. Serine 97-103 AKT serine/threonine kinase 1 Homo sapiens 68-72 19531639-4 2009 In particular, PKC phosphorylates PED/PEA-15 at Ser(104) and CAM kinase II or Akt at Ser(116), modifying its stability. Serine 85-88 AKT serine/threonine kinase 1 Homo sapiens 78-81 19615971-2 2009 Treatment of T84 cells with the selective inhibitor of EGF receptor (EGFR) tyrosine kinase AG1478 abrogated Akt phosphorylation on Ser(473) induced by either carbachol or EGF, indicating that carbachol-induced Akt activation is mediated through EGFR transactivation. Serine 131-134 AKT serine/threonine kinase 1 Homo sapiens 108-111 19574087-4 2009 Western blot showed that both total PTEN and membrane-bound PTEN were induced by TSA treatment, whereas phosphorylation level (Ser 473) of AKT was correspondingly down-regulated by TSA treatment. Serine 127-130 AKT serine/threonine kinase 1 Homo sapiens 139-142 19482076-0 2009 ROS-driven Akt dephosphorylation at Ser-473 is involved in 4-HPR-mediated apoptosis in NB4 cells. Serine 36-39 AKT serine/threonine kinase 1 Homo sapiens 11-14 19261608-10 2009 Thus, Bcr-Abl represses the expression of PHLPP1 and PHLPP2 and continuously activates Akt1, -2, and -3 via phosphorylation on Ser-473, resulting in the proliferation of CML cells. Serine 127-130 AKT serine/threonine kinase 1 Homo sapiens 87-103 19696224-8 2009 In addition, beta-catenin significantly increased the rate of phosphorylation at Bax serine(184), an Akt-specific target. Serine 85-91 AKT serine/threonine kinase 1 Homo sapiens 101-104 19261608-7 2009 The Abl kinase inhibitors and depletion of Bcr-Abl induced the expression of PHLPP1 and PHLPP2, which dephosphorylated Ser-473 on Akt1, -2, and -3, resulting in inhibited proliferation of CML cells. Serine 119-122 AKT serine/threonine kinase 1 Homo sapiens 130-146 19477925-6 2009 E(2) inhibits ATR activation through rapid PI3K/AKT signaling: AKT phosphorylates TopBP1 at Serine 1159, thereby preventing the enhanced association of ATR with TopBP1 after DNA damage. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 48-51 19359389-7 2009 By contrast, siRNA-mediated knockdown of Akt1 did not affect AMPKalpha1 phosphorylation, but it abolished leptin-induced phosphorylation of Akt-Ser(473) and eNOS-Ser(1177), suggesting that Akt functions downstream of AMPKalpha1. Serine 144-147 AKT serine/threonine kinase 1 Homo sapiens 41-45 19359389-7 2009 By contrast, siRNA-mediated knockdown of Akt1 did not affect AMPKalpha1 phosphorylation, but it abolished leptin-induced phosphorylation of Akt-Ser(473) and eNOS-Ser(1177), suggesting that Akt functions downstream of AMPKalpha1. Serine 144-147 AKT serine/threonine kinase 1 Homo sapiens 41-44 19359389-7 2009 By contrast, siRNA-mediated knockdown of Akt1 did not affect AMPKalpha1 phosphorylation, but it abolished leptin-induced phosphorylation of Akt-Ser(473) and eNOS-Ser(1177), suggesting that Akt functions downstream of AMPKalpha1. Serine 144-147 AKT serine/threonine kinase 1 Homo sapiens 140-143 19359389-7 2009 By contrast, siRNA-mediated knockdown of Akt1 did not affect AMPKalpha1 phosphorylation, but it abolished leptin-induced phosphorylation of Akt-Ser(473) and eNOS-Ser(1177), suggesting that Akt functions downstream of AMPKalpha1. Serine 162-165 AKT serine/threonine kinase 1 Homo sapiens 41-45 19359389-7 2009 By contrast, siRNA-mediated knockdown of Akt1 did not affect AMPKalpha1 phosphorylation, but it abolished leptin-induced phosphorylation of Akt-Ser(473) and eNOS-Ser(1177), suggesting that Akt functions downstream of AMPKalpha1. Serine 162-165 AKT serine/threonine kinase 1 Homo sapiens 41-44 19573808-4 2009 Surprisingly, the latter effects required phosphorylation of EphA2 on serine 897 by Akt, and S897A mutation abolished ligand-independent promotion of cell motility. Serine 70-76 AKT serine/threonine kinase 1 Homo sapiens 84-87 19477925-6 2009 E(2) inhibits ATR activation through rapid PI3K/AKT signaling: AKT phosphorylates TopBP1 at Serine 1159, thereby preventing the enhanced association of ATR with TopBP1 after DNA damage. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 63-66 19661333-6 2009 Surprisingly, curcumin induced phosphorylation of Akt(Ser 473); this effect may be attributed to a decrease in levels of the PHLPP1 phosphatase, an inhibitor of Akt. Serine 54-57 AKT serine/threonine kinase 1 Homo sapiens 50-53 19006485-5 2009 On the basis of the sequence around Ser-32 and the finding that phosphorylation is inhibited by the PI3K (phosphoinositide 3-kinase) inhibitor wortmannin, it is likely that Akt is the kinase for Ser-32. Serine 36-39 AKT serine/threonine kinase 1 Homo sapiens 173-176 19006485-5 2009 On the basis of the sequence around Ser-32 and the finding that phosphorylation is inhibited by the PI3K (phosphoinositide 3-kinase) inhibitor wortmannin, it is likely that Akt is the kinase for Ser-32. Serine 195-198 AKT serine/threonine kinase 1 Homo sapiens 173-176 19638206-8 2009 p110 alpha overexpression was associated with increased phosphorylation of AKT-Ser 473 and with the proliferation marker Ki-67. Serine 79-82 AKT serine/threonine kinase 1 Homo sapiens 75-78 19584241-7 2009 Moreover, the antiapoptotic protein Akt/protein kinase B was down-regulated and also dephosphorylated at residue Ser(473) in a dose- and time-dependent manner and NB cells entered apoptotic cell death. Serine 113-116 AKT serine/threonine kinase 1 Homo sapiens 36-39 18996613-6 2009 OPN stimulation increased the phosphorylation of focal adhesion kinase (FAK), p85 subunit of PI3K, serine 473 of Akt and ERK. Serine 99-105 AKT serine/threonine kinase 1 Homo sapiens 113-116 19406987-5 2009 This resulted in a significant enhancement of Akt (ser(473)) phosphorylation and T-cell receptor-induced proliferative activity of CD8+CD28-CD27- T cells. Serine 51-54 AKT serine/threonine kinase 1 Homo sapiens 46-49 19303913-0 2009 Phosphorylation of FADD (Fas-associated death domain protein) at serine 194 is increased in the prefrontal cortex of opiate abusers: relation to mitogen activated protein kinase, phosphoprotein enriched in astrocytes of 15 kDa, and Akt signaling pathways involved in neuroplasticity. Serine 65-71 AKT serine/threonine kinase 1 Homo sapiens 232-235 19477675-9 2009 But capacitation seems to be more complex with the contribution of other kinases (from the PI3K/Akt pathway and phosphotyrosine kinases) towards the phosphorylation of other Ser/Thr and Tyr proteins. Serine 174-177 AKT serine/threonine kinase 1 Homo sapiens 96-99 19351806-9 2009 Visfatin increased total Akt and Ser(473)-phospho-Akt expression with concomitant rises in eNOS phosphorylation at Ser(1177); these effects were blocked by LY-2940002. Serine 33-36 AKT serine/threonine kinase 1 Homo sapiens 50-53 19376254-5 2009 Western blotting showed that 1.0microg/ml LPS enhanced phosphorylation of both Akt at Ser 473 and nuclear factor-kappa B (NF-kappaB) p65 at Ser 536. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 79-82 18996613-9 2009 The OPN-mediated increases in IKK alpha/beta, IkappaBalpha and p65 Ser(536) phosphorylation were inhibited by Ly294002, Akt inhibitor and PD98059. Serine 67-70 AKT serine/threonine kinase 1 Homo sapiens 120-123 19158829-2 2009 As full activation of Akt requires phosphorylation on both threonine 308 (Thr308) and serine 473 (Ser473) residues, we studied the level of phosphorylation on the both sites in 58 AML samples by flow cytometry. Serine 86-92 AKT serine/threonine kinase 1 Homo sapiens 22-25 19324870-1 2009 PHLPP2 (PH domain leucine-rich repeat protein phosphatase 2) terminates Akt and protein kinase C (PKC) activity by specifically dephosphorylating these kinases at a key regulatory site, the hydrophobic motif (Ser-473 in Akt1). Serine 209-212 AKT serine/threonine kinase 1 Homo sapiens 72-75 19284309-7 2009 Importantly, time-dependent signaling consequences associated with ILK silencing, including suppression of phosphorylated (serine 473)-AKT and changes in mTOR expression, were observed independently of ILK suppression when the target cells were exposed to cationic lipids following nucleofection-based delivery of ILK siRNA. Serine 123-129 AKT serine/threonine kinase 1 Homo sapiens 135-138 19324870-1 2009 PHLPP2 (PH domain leucine-rich repeat protein phosphatase 2) terminates Akt and protein kinase C (PKC) activity by specifically dephosphorylating these kinases at a key regulatory site, the hydrophobic motif (Ser-473 in Akt1). Serine 209-212 AKT serine/threonine kinase 1 Homo sapiens 220-224 19324870-3 2009 Depletion of endogenous PHLPP2 variants in breast cancer cells revealed the Ser-1016 variant is less functional toward both Akt and PKC. Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 124-127 19435822-7 2009 Indeed, distinct from its known ability to induce c-fos and fra1 and to stabilize c-Jun, Akt appeared to directly regulate the transcriptional activity of c-Jun independently of the phosphorylation sites targeted by c-Jun NH(2)-terminal kinase (Ser(63)/Ser(73)) and glycogen synthase kinase-3 (Thr(239)). Serine 245-248 AKT serine/threonine kinase 1 Homo sapiens 89-92 19240153-6 2009 When cells were treated with 100 nm R5020 for 1 and 24 h, the levels of phospho(Ser 473)-AKT increased. Serine 80-83 AKT serine/threonine kinase 1 Homo sapiens 89-92 19477150-4 2009 Here, we demonstrate that PKB/Akt directly phosphorylates NR2C on serine 1096 (S1096). Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 26-29 19477150-4 2009 Here, we demonstrate that PKB/Akt directly phosphorylates NR2C on serine 1096 (S1096). Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 30-33 19339247-7 2009 Moreover, TNFalpha enhanced FLIP(L) serine phosphorylation, which was increased by activated Akt-1. Serine 36-42 AKT serine/threonine kinase 1 Homo sapiens 93-98 19339247-8 2009 Serine 273, a putative Akt-1 phosphorylation site in FLIP(L), was critical for the activation-induced reduction of FLIP(L). Serine 0-6 AKT serine/threonine kinase 1 Homo sapiens 23-28 19435822-7 2009 Indeed, distinct from its known ability to induce c-fos and fra1 and to stabilize c-Jun, Akt appeared to directly regulate the transcriptional activity of c-Jun independently of the phosphorylation sites targeted by c-Jun NH(2)-terminal kinase (Ser(63)/Ser(73)) and glycogen synthase kinase-3 (Thr(239)). Serine 253-256 AKT serine/threonine kinase 1 Homo sapiens 89-92 19383978-3 2009 This mTOR complex also promotes the constitutive phosphorylation of proline-directed serine or threonine sites in the turn motif of Akt and protein kinase C isoforms. Serine 85-91 AKT serine/threonine kinase 1 Homo sapiens 132-135 19229860-4 2009 Magnolol treatment (60 microM) also caused a decrease in Ser((136)) phosphorylation of Bad (a proapoptotic protein), which is a downstream target of Akt. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 149-152 18771813-4 2009 TGF-beta1 stimulation increased phosphorylation of p85alpha subunit of phosphatidylinositol 3-kinase (PI3K) and Ser(473) of Akt was determined. Serine 112-115 AKT serine/threonine kinase 1 Homo sapiens 124-127 19162005-10 2009 Mutation of RGC-32 protein at Ser 45 and Ser 47 prevented Akt mediated phosphorylation. Serine 30-33 AKT serine/threonine kinase 1 Homo sapiens 58-61 19162005-10 2009 Mutation of RGC-32 protein at Ser 45 and Ser 47 prevented Akt mediated phosphorylation. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 58-61 19326566-2 2009 Activation of insulin receptor beta and insulin receptor substrate 1 was tested in pull-down assays, and phosphorylation of Protein kinase B (PKB/akt) on ser 473 was measured by Western blot. Serine 154-157 AKT serine/threonine kinase 1 Homo sapiens 142-149 19073147-4 2009 CCL5 stimulation increased phosphorylation of the p85alpha subunit of phosphatidylinositol 3-kinase (PI3K) and serine 473 of Akt. Serine 111-117 AKT serine/threonine kinase 1 Homo sapiens 125-128 19270695-4 2009 We show further that Akt1 phosphorylates Skp2 at Ser 72, which is required to disrupt the interaction between Cdh1 and Skp2. Serine 49-52 AKT serine/threonine kinase 1 Homo sapiens 21-25 19270695-5 2009 In addition, we show that Ser 72 is localized within a putative nuclear localization sequence and that phosphorylation of Ser 72 by Akt leads to cytoplasmic translocation of Skp2. Serine 122-125 AKT serine/threonine kinase 1 Homo sapiens 132-135 19073147-8 2009 Furthermore, the CCL5-mediated increases in p65 Ser(536) phosphorylation were inhibited by Ly294002 and Akt inhibitor. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 104-107 18948408-5 2009 Reduction in expression of either p85alpha or S6K1 achieved with small interfering RNA in the presence of myristoylated Akt rescued 3T3-L1 adipocytes from the insulin resistance induced by serine phosphorylation of IRS-1 and completely restored insulin-stimulated activation of PI 3-kinase and glucose uptake. Serine 189-195 AKT serine/threonine kinase 1 Homo sapiens 120-123 18948408-3 2009 In this study, we transduced fully differentiated 3T3-L1 adipocytes with a constitutively active myristoylated Akt that led to hyperactivation of mammalian target of rapamycin and p70 S6 kinase (S6K1), increased serine phosphorylation of IRS-1, and reduction in insulin-stimulated phosphatidylinositol (PI) 3-kinase activity and glucose transport. Serine 212-218 AKT serine/threonine kinase 1 Homo sapiens 111-114 19112161-7 2009 In contrast, phosphorylation of protein kinase B/Akt (Thr(308) and Ser(473)) tended to decrease, and downstream targets linked to hypertrophy (p70 ribosomal S6 kinase and 4E binding protein 1) were unchanged after exercise and recovery. Serine 67-70 AKT serine/threonine kinase 1 Homo sapiens 49-52 18694847-5 2009 Almost all Akt kinase that translocates to the nucleus shows a marked phosphorylation on serine 473. Serine 89-95 AKT serine/threonine kinase 1 Homo sapiens 11-14 20108211-11 2009 It also increased NOS-3 phosphorylation on serine-1177, and increased Akt serine phosphorylation. Serine 74-80 AKT serine/threonine kinase 1 Homo sapiens 70-73 19122209-4 2009 Ser(245) was also found to be a substrate for AKT and the resulting Ser(245) phosphorylation induced the TTP-14-3-3 interaction. Serine 0-3 AKT serine/threonine kinase 1 Homo sapiens 46-49 19122209-4 2009 Ser(245) was also found to be a substrate for AKT and the resulting Ser(245) phosphorylation induced the TTP-14-3-3 interaction. Serine 68-71 AKT serine/threonine kinase 1 Homo sapiens 46-49 19122209-6 2009 These results suggest that TTP Ser(245) is the principal target for the modulation of this protein via the AKT signalling cascade. Serine 31-34 AKT serine/threonine kinase 1 Homo sapiens 107-110 19176382-5 2009 In addition, we have found that activated Akt can bind and phosphorylate GGAP2 at serine 629, which enhances GTP binding by GGAP2. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 42-45 19143635-7 2009 The growth-factor-stimulated kinase activity of mTORC2 [also known as the mTOR-rictor (rapamycin-insensitive companion of mTOR) complex], which normally enhances Akt signalling by phosphorylating its hydrophobic motif (Ser(473)), was found to be defective in cells lacking the TSC1-TSC2 complex. Serine 219-222 AKT serine/threonine kinase 1 Homo sapiens 162-165 19109987-8 2009 Finally, a single Cr(VI) challenge resulted in a rapid and persistent increase in the number of airways immunoreactive for phosphorylation of the survival signaling protein Akt, on serine 473. Serine 181-187 AKT serine/threonine kinase 1 Homo sapiens 173-176 18812223-5 2008 Akt also became phosphorylated on Ser(473) in cells subjected to repetitive oxidative stress. Serine 34-37 AKT serine/threonine kinase 1 Homo sapiens 0-3 19064616-8 2009 Western blots revealed that compared with -LMN myocytes, +LMN myocytes showed a significant increase in Akt phosphorylation at Ser-473, which was prevented by LY. Serine 127-130 AKT serine/threonine kinase 1 Homo sapiens 104-107 18992820-1 2009 Huntingtin is phosphorylated on serine-421 (S421) by the pro-survival signaling protein kinases Akt and SGK. Serine 32-38 AKT serine/threonine kinase 1 Homo sapiens 96-99 18836482-3 2009 Using an in vitro kinase assay, we found that PKB can directly phosphorylate eIF4B on serine 422 (ser422). Serine 86-92 AKT serine/threonine kinase 1 Homo sapiens 46-49 19009553-6 2009 US stimulation increased the phosphorylation of p85 subunit of PI3K and serine 473 of Akt. Serine 72-78 AKT serine/threonine kinase 1 Homo sapiens 86-89 20641681-0 2004 NFluc-FHA2-Aktpep-CFluc The serine/threonine kinase (Akt), also known as protein kinase B (PKB), is an enzyme that covalently attaches ATP-phosphate groups to the serine/threonine on protein substrates to alter the activity of the targeted protein (1, 2). Serine 28-34 AKT serine/threonine kinase 1 Homo sapiens 11-14 20641681-0 2004 NFluc-FHA2-Aktpep-CFluc The serine/threonine kinase (Akt), also known as protein kinase B (PKB), is an enzyme that covalently attaches ATP-phosphate groups to the serine/threonine on protein substrates to alter the activity of the targeted protein (1, 2). Serine 28-34 AKT serine/threonine kinase 1 Homo sapiens 91-94 20641681-4 2004 The activation of Akt is completed via the phosphorylation of Thr-308 and Ser-473 (i.e., in Akt1), leading to increased Akt activity toward a variety of downstream substrates, such as the family of forkhead transcription factors for inhibiting tumor proliferations, the mammalian target of rapamycin (mTOR) for modulating protein synthesis, and the Bcl-xL/Bcl-2-associated death promoter (BAD) for uncontrolled proliferation (3, 5). Serine 74-77 AKT serine/threonine kinase 1 Homo sapiens 18-21 20641681-4 2004 The activation of Akt is completed via the phosphorylation of Thr-308 and Ser-473 (i.e., in Akt1), leading to increased Akt activity toward a variety of downstream substrates, such as the family of forkhead transcription factors for inhibiting tumor proliferations, the mammalian target of rapamycin (mTOR) for modulating protein synthesis, and the Bcl-xL/Bcl-2-associated death promoter (BAD) for uncontrolled proliferation (3, 5). Serine 74-77 AKT serine/threonine kinase 1 Homo sapiens 92-96 20641681-4 2004 The activation of Akt is completed via the phosphorylation of Thr-308 and Ser-473 (i.e., in Akt1), leading to increased Akt activity toward a variety of downstream substrates, such as the family of forkhead transcription factors for inhibiting tumor proliferations, the mammalian target of rapamycin (mTOR) for modulating protein synthesis, and the Bcl-xL/Bcl-2-associated death promoter (BAD) for uncontrolled proliferation (3, 5). Serine 74-77 AKT serine/threonine kinase 1 Homo sapiens 92-95 19036927-10 2008 E4ORF1 augments survival of PECs in part by maintaining FGF-2/FGF-R1 signaling and through tonic Ser-473 phosphorylation of Akt, thereby activating the mTOR and NF-kappaB pathways. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 124-127 18931031-5 2008 HCMVEC exposed to 500 microM H2O2 had increased Akt phosphorylation within 10 min at both Ser-473 and Thr-308 sites, an effect blocked by the phosphatidylinositol 3-kinase inhibitor LY-294002. Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 48-51 18931031-6 2008 H2O2 also induced NO generation that was associated with NOS3 Ser-1177 site phosphorylation and Thr-495 dephosphorylation, with Ser-1177 effects attenuated by LY-294002 and an Akt inhibitor, Akt/PKB signaling inhibitor-2 (API-2). Serine 128-131 AKT serine/threonine kinase 1 Homo sapiens 176-179 18720410-5 2008 BMP-2 increased the phosphorylation of p85 subunit of PI3K and serine 473 of Akt. Serine 63-69 AKT serine/threonine kinase 1 Homo sapiens 77-80 19074832-4 2008 Ionizing radiation triggered Thr(308) and Ser(473) phosphorylation of Akt. Serine 42-45 AKT serine/threonine kinase 1 Homo sapiens 70-73 19074832-7 2008 Interestingly, inhibition of p38 MAPK completely attenuated radiation-induced Ser(473) phosphorylation of Akt but did not affect Thr(308) phosphorylation. Serine 78-81 AKT serine/threonine kinase 1 Homo sapiens 106-109 19074832-8 2008 Conversely, overexpression of p38 MAPK enhanced Ser(473) phosphorylation of Akt in response to radiation. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 76-79 19074832-10 2008 Ectopic expression of RacN17, dominant-negative form of Rac1, inhibited p38 MAPK activation and Ser(473) phosphorylation of Akt. Serine 96-99 AKT serine/threonine kinase 1 Homo sapiens 124-127 19074832-12 2008 Inhibition of c-Src attenuated Rac1 and p38 MAPK activations and Ser(473) phosphorylation of Akt. Serine 65-68 AKT serine/threonine kinase 1 Homo sapiens 93-96 18838385-5 2008 In cultured cardiac myocytes, increased expression of adenylyl cyclase VI activates Akt by phosphorylation at serine 473 and threonine 308 and is associated with increased nuclear phospho-Akt. Serine 110-116 AKT serine/threonine kinase 1 Homo sapiens 84-87 19079291-8 2009 It also diminished insulin-stimulated tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt without affecting the phosphorylation of IR, ERK1/2, p38, and JNK. Serine 76-82 AKT serine/threonine kinase 1 Homo sapiens 102-105 18691253-7 2008 Overexpression of RhoH leads to dephosphorylation of Bad at Serine 75 residue possibly through deactivation of Rac. Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 111-114 18845784-10 2008 Phospho(Ser/Thr)-Akt substrate (PAS) phosphorylation (often used as a marker for AS160) was unchanged immediately postexercise and increased at 1 h (P <or= 0.05) and 2 h postexercise (P = 0.07). Serine 8-11 AKT serine/threonine kinase 1 Homo sapiens 17-20 18557931-6 2008 As we have recently shown that protein kinase B/Akt is involved in the TNFalpha signalling pathway and promotes cell survival and skin carcinogenesis, we measured activatory phosphorylations of Akt (Ser-473 and Thr-308) and the signalling via related pathways Erk 1/2, p38 and p70-S6K. Serine 199-202 AKT serine/threonine kinase 1 Homo sapiens 48-51 18557931-6 2008 As we have recently shown that protein kinase B/Akt is involved in the TNFalpha signalling pathway and promotes cell survival and skin carcinogenesis, we measured activatory phosphorylations of Akt (Ser-473 and Thr-308) and the signalling via related pathways Erk 1/2, p38 and p70-S6K. Serine 199-202 AKT serine/threonine kinase 1 Homo sapiens 194-197 18818526-4 2008 A series of steps leading to metastatic properties can be initiated upon activation of AKT by phosphorylation on Ser-473. Serine 113-116 AKT serine/threonine kinase 1 Homo sapiens 87-90 18721797-0 2008 Temperature sensitivity of phospho-Ser(473)-PKB/AKT. Serine 35-38 AKT serine/threonine kinase 1 Homo sapiens 44-47 18721797-0 2008 Temperature sensitivity of phospho-Ser(473)-PKB/AKT. Serine 35-38 AKT serine/threonine kinase 1 Homo sapiens 48-51 18721797-3 2008 Here, we determined that p-Ser(473)-PKB/Akt but not p-Thr(308)-PKB/Akt phosphorylation is highly temperature sensitive. Serine 27-30 AKT serine/threonine kinase 1 Homo sapiens 36-39 18721797-4 2008 p-Ser(473)-PKB/Akt phosphorylation was rapidly reduced to levels below 50% on exposure to 20-25 degrees C in murine and human cell lines including cells expressing constitutively active PI3K or lacking PTEN. Serine 2-5 AKT serine/threonine kinase 1 Homo sapiens 11-14 18721797-5 2008 Down-regulation of p-Ser(473)-PKB/Akt was reversible and re-exposure to physiological temperature resulted in increased p-Ser(473)-PKB/Akt phosphorylation levels. Serine 21-24 AKT serine/threonine kinase 1 Homo sapiens 30-33 18721797-5 2008 Down-regulation of p-Ser(473)-PKB/Akt was reversible and re-exposure to physiological temperature resulted in increased p-Ser(473)-PKB/Akt phosphorylation levels. Serine 21-24 AKT serine/threonine kinase 1 Homo sapiens 34-37 18721797-5 2008 Down-regulation of p-Ser(473)-PKB/Akt was reversible and re-exposure to physiological temperature resulted in increased p-Ser(473)-PKB/Akt phosphorylation levels. Serine 21-24 AKT serine/threonine kinase 1 Homo sapiens 131-134 18721797-5 2008 Down-regulation of p-Ser(473)-PKB/Akt was reversible and re-exposure to physiological temperature resulted in increased p-Ser(473)-PKB/Akt phosphorylation levels. Serine 21-24 AKT serine/threonine kinase 1 Homo sapiens 135-138 18721797-5 2008 Down-regulation of p-Ser(473)-PKB/Akt was reversible and re-exposure to physiological temperature resulted in increased p-Ser(473)-PKB/Akt phosphorylation levels. Serine 122-125 AKT serine/threonine kinase 1 Homo sapiens 30-33 18721797-5 2008 Down-regulation of p-Ser(473)-PKB/Akt was reversible and re-exposure to physiological temperature resulted in increased p-Ser(473)-PKB/Akt phosphorylation levels. Serine 122-125 AKT serine/threonine kinase 1 Homo sapiens 34-37 18721797-5 2008 Down-regulation of p-Ser(473)-PKB/Akt was reversible and re-exposure to physiological temperature resulted in increased p-Ser(473)-PKB/Akt phosphorylation levels. Serine 122-125 AKT serine/threonine kinase 1 Homo sapiens 131-134 18721797-5 2008 Down-regulation of p-Ser(473)-PKB/Akt was reversible and re-exposure to physiological temperature resulted in increased p-Ser(473)-PKB/Akt phosphorylation levels. Serine 122-125 AKT serine/threonine kinase 1 Homo sapiens 135-138 18721797-6 2008 Phosphatase activity at low temperature was sustained at 75% baseline level and phosphatase inhibition prevented p-Ser(473)-PKB/Akt dephosphorylation induced by the low temperature shift. Serine 115-118 AKT serine/threonine kinase 1 Homo sapiens 124-127 18721797-6 2008 Phosphatase activity at low temperature was sustained at 75% baseline level and phosphatase inhibition prevented p-Ser(473)-PKB/Akt dephosphorylation induced by the low temperature shift. Serine 115-118 AKT serine/threonine kinase 1 Homo sapiens 128-131 18721797-7 2008 Interestingly temperature-dependent deregulation of the p-Ser(473)-PKB/Akt status was also observed in response to irradiation. Serine 58-61 AKT serine/threonine kinase 1 Homo sapiens 67-70 18721797-7 2008 Interestingly temperature-dependent deregulation of the p-Ser(473)-PKB/Akt status was also observed in response to irradiation. Serine 58-61 AKT serine/threonine kinase 1 Homo sapiens 71-74 18721797-8 2008 Thus our data demonstrate that minimal additional stress factors deregulate the PI3K/Akt-survival pathway and the p-Ser(473)-PKB/Akt status as experimental endpoint. Serine 116-119 AKT serine/threonine kinase 1 Homo sapiens 125-128 18622039-5 2008 Phosphorylation of Ser-1177 by Akt led to an increase (>50%) in maximal O(2)* generation from eNOS. Serine 19-22 AKT serine/threonine kinase 1 Homo sapiens 31-34 18829572-4 2008 Consistent with this, we find that levels of p-AKT (Ser(473)) induced by mutant Ras or knockdown of PTEN were dramatically increased by addition of mutant PIK3CA. Serine 52-55 AKT serine/threonine kinase 1 Homo sapiens 47-50 18676874-7 2008 These results suggest that PAK1-specific paxillin phosphorylation at Ser(273) is critically involved in the positive-feedback regulation of the Rac-PAK1 pathway and may contribute to sustained enhancement of the EC barrier caused by oxidized phospholipids. Serine 69-72 AKT serine/threonine kinase 1 Homo sapiens 144-147 18615850-9 2008 Insulin-stimulated phosphorylation of Akt-Ser(473) or Akt-Thr(308) was comparable in type 2 diabetic and non-diabetic muscles, and unaltered by the addition of globular adiponectin in either group. Serine 42-45 AKT serine/threonine kinase 1 Homo sapiens 38-41 18667411-6 2008 Comparison across tissue specimens showed an >20% decrease of protein kinase B (AKT) Ser-473 (p < 0.002) and myristoylated alanine-rich C-kinase substrate protein Ser-152/156 (p < 0.0001) within the first 90-min postexcision. Serine 88-91 AKT serine/threonine kinase 1 Homo sapiens 83-86 18687633-7 2008 Persistence of phosphorylation for AKT Ser-473 following ligand stimulation was found for the mutant. Serine 39-42 AKT serine/threonine kinase 1 Homo sapiens 35-38 18687633-8 2008 These data suggest that a higher proportion of the EGFR mutant carcinoma cells may exhibit activation of the phosphatidylinositol 3-kinase/protein kinase B (AKT)/mammalian target of rapamycin (MTOR) pathway through Tyr-1148 and Tyr-1068 and suppression of IRS-1 Ser-612, altered heterodimerization with ERBB2, reduced response to transforming growth factor beta suppression, and reduced ubiquitination/degradation of the EGFR through EGFR Tyr-1045, thus providing a survival advantage. Serine 262-265 AKT serine/threonine kinase 1 Homo sapiens 157-160 18652836-7 2008 Together, our results show that Src kinase mediates ERbeta-induced increases in phosphorylation levels of nNOS at Ser(1412) and NO production by activating the PI3K/Akt pathway. Serine 114-117 AKT serine/threonine kinase 1 Homo sapiens 165-168 18794113-5 2008 On expanding our analysis to other Akt targets, using isogenic LKB1 knockdown cell line pairs and a phospho-specific antibody microarray, we observed that there was a requirement for LKB1 in the phosphorylation of other Akt downstream targets, including Ask1 (Ser(83)), Bad (Ser(136)), FoxO1 (Ser(319)), FoxO4 (Ser(197)), and glycogen synthase kinase 3beta (GSK3beta; Ser(9)). Serine 260-263 AKT serine/threonine kinase 1 Homo sapiens 220-223 18759402-8 2008 The RDF design allows inclusion of extended binding determinants to maximize recognition by the cognate kinase, which has now permitted the construction of chemosensors for a variety of representative Ser/Thr (PKC alpha, PKC betaIota, PKC delta, Pim2, Akt1, MK2, and PKA) as well as receptor (IRK) and nonreceptor (Src, Abl) Tyr kinases with greatly enhanced selectivity. Serine 201-204 AKT serine/threonine kinase 1 Homo sapiens 252-256 18794113-5 2008 On expanding our analysis to other Akt targets, using isogenic LKB1 knockdown cell line pairs and a phospho-specific antibody microarray, we observed that there was a requirement for LKB1 in the phosphorylation of other Akt downstream targets, including Ask1 (Ser(83)), Bad (Ser(136)), FoxO1 (Ser(319)), FoxO4 (Ser(197)), and glycogen synthase kinase 3beta (GSK3beta; Ser(9)). Serine 275-278 AKT serine/threonine kinase 1 Homo sapiens 220-223 18794113-5 2008 On expanding our analysis to other Akt targets, using isogenic LKB1 knockdown cell line pairs and a phospho-specific antibody microarray, we observed that there was a requirement for LKB1 in the phosphorylation of other Akt downstream targets, including Ask1 (Ser(83)), Bad (Ser(136)), FoxO1 (Ser(319)), FoxO4 (Ser(197)), and glycogen synthase kinase 3beta (GSK3beta; Ser(9)). Serine 275-278 AKT serine/threonine kinase 1 Homo sapiens 220-223 18794113-5 2008 On expanding our analysis to other Akt targets, using isogenic LKB1 knockdown cell line pairs and a phospho-specific antibody microarray, we observed that there was a requirement for LKB1 in the phosphorylation of other Akt downstream targets, including Ask1 (Ser(83)), Bad (Ser(136)), FoxO1 (Ser(319)), FoxO4 (Ser(197)), and glycogen synthase kinase 3beta (GSK3beta; Ser(9)). Serine 275-278 AKT serine/threonine kinase 1 Homo sapiens 220-223 18794113-5 2008 On expanding our analysis to other Akt targets, using isogenic LKB1 knockdown cell line pairs and a phospho-specific antibody microarray, we observed that there was a requirement for LKB1 in the phosphorylation of other Akt downstream targets, including Ask1 (Ser(83)), Bad (Ser(136)), FoxO1 (Ser(319)), FoxO4 (Ser(197)), and glycogen synthase kinase 3beta (GSK3beta; Ser(9)). Serine 275-278 AKT serine/threonine kinase 1 Homo sapiens 220-223 18598695-13 2008 These data indicate that caveolin-1 specifies filamin A as a novel target for Akt-mediated filamin A Ser-2152 phosphorylation thus mediating the effects of caveolin-1 on IGF-I-induced cancer cell migration. Serine 101-104 AKT serine/threonine kinase 1 Homo sapiens 78-81 18617528-10 2008 Blocking Akt activation abolished the initial rise of eNOS Ser(1179) phosphorylation after H(2)O(2) treatment. Serine 59-62 AKT serine/threonine kinase 1 Homo sapiens 9-12 18617528-11 2008 In long term H(2)O(2)-treated cells where Akt was deactivated, significant amounts of Ser(1179)-phosphorylated eNOS remained. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 42-45 18617528-13 2008 Taken together, these studies revealed that Akt and AMPK orchestrated a bidirectional action on eNOS Ser(1179) phosphorylation in H(2)O(2)-treated cells. Serine 101-104 AKT serine/threonine kinase 1 Homo sapiens 44-47 18617528-2 2008 Reactive oxygen species such as H(2)O(2) was reported to activate Akt, leading to increased eNOS Ser(1179) phosphorylation and activity. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 66-69 18617528-8 2008 Parallel to the alterations of eNOS Ser(1179) phosphorylation, Akt was transiently activated by H(2)O(2) and subsequently became dormant. Serine 36-39 AKT serine/threonine kinase 1 Homo sapiens 63-66 18678787-4 2008 High glucose simultaneously augmented Akt phosphorylation at Ser 473, as well as Thr 308 in the human vascular smooth muscle cells. Serine 61-64 AKT serine/threonine kinase 1 Homo sapiens 38-41 18556572-6 2008 Cilostazol increased phosphorylation of Akt at Ser(473) and of endothelial nitric oxide synthase (eNOS) at Ser(1177), with a dose-dependent increase in Sirt1 expression. Serine 47-50 AKT serine/threonine kinase 1 Homo sapiens 40-43 18562319-4 2008 Akt regulates this IRES activity by inducing phosphorylation of hnRNP A1 on serine 199. Serine 76-82 AKT serine/threonine kinase 1 Homo sapiens 0-3 18826385-6 2008 In contrast, AKT was expressed in 83.3% of ameloblastomas, showing high expression of the p-thr(308)AKT and p-ser(473) AKT forms in 93.3% and 56.6% of cases, respectively. Serine 110-113 AKT serine/threonine kinase 1 Homo sapiens 13-16 18487214-6 2008 Further analysis indicated that the p38 MAPK inhibitor SB 203580 inhibited EGF-stimulated phosphorylation of Akt on serine 473, which may be responsible for the effect SB 203580 has on CTB motility. Serine 116-122 AKT serine/threonine kinase 1 Homo sapiens 109-112 18495429-10 2008 In contrast, silencing Epac1 gene expression reduced Akt(Ser-473) kinase activity and p-Akt(Ser-473) levels in plasma membranes, but showed negligible effects on nuclear activity. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 53-56 18615096-4 2008 Huntingtin is phosphorylated at serine 421 by the kinase Akt but the role of this modification is unknown. Serine 32-38 AKT serine/threonine kinase 1 Homo sapiens 57-60 18443205-11 2008 siRNA-mediated IKKbeta reduction prevented TNF-alpha-induced insulin resistance on Akt Ser(473) and Thr(308) phosphorylation and phosphorylation of the 160-kDa Akt substrate AS160. Serine 87-90 AKT serine/threonine kinase 1 Homo sapiens 83-86 18467333-5 2008 Additionally, the Pim kinases phosphorylate Mdm2 in vitro and in cultured cells at Ser(166) and Ser(186), two previously identified targets of other signaling pathways, including Akt. Serine 83-86 AKT serine/threonine kinase 1 Homo sapiens 179-182 18586515-5 2008 EPO stimulated the phosphorylation of Akt at serine-473 and co-incubation of the Akt/PI-3 kinase pathway inhibitor LY294002 with EPO abolished its effects on Akt phosphorylation and axonal growth. Serine 45-51 AKT serine/threonine kinase 1 Homo sapiens 38-41 18480058-8 2008 In Western blot analysis, a low concentration of AYPGKF, but not SFLLRN, stimulated phosphorylation of Akt on serine 473. Serine 110-116 AKT serine/threonine kinase 1 Homo sapiens 103-106 18663626-4 2008 It was also found that shear-induced activation of the Akt pathway was significantly decreased in cells grown without serine. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 55-58 18426911-4 2008 Furthermore, while RNA interference-directed silencing of Rictor results in the expected attenuation of Akt phosphorylation at serine 473, depletion of Myo1c is without effect. Serine 127-133 AKT serine/threonine kinase 1 Homo sapiens 104-107 18632646-6 2008 Our results show that inhibiting PI3K activity reduces phosphorylation of AKT at serine 473. Serine 81-87 AKT serine/threonine kinase 1 Homo sapiens 74-77 18397976-8 2008 During the clamp the phosphorylation of protein kinase B/AKT at serine(473) in proportion to the total amount of protein kinase B/AKT was significantly lower. Serine 64-70 AKT serine/threonine kinase 1 Homo sapiens 57-60 18397976-8 2008 During the clamp the phosphorylation of protein kinase B/AKT at serine(473) in proportion to the total amount of protein kinase B/AKT was significantly lower. Serine 64-70 AKT serine/threonine kinase 1 Homo sapiens 130-133 18397976-10 2008 CONCLUSIONS: Fasting for 62 h decreases insulin-mediated peripheral glucose uptake with lower phosphorylation of AKT at serine(473). Serine 120-126 AKT serine/threonine kinase 1 Homo sapiens 113-116 18426911-1 2008 Insulin signaling through phosphatidylinositol 3-kinase (PI 3-kinase) activates the protein kinase Akt through phosphorylation of its threonine 308 and serine 473 residues by the PDK1 protein kinase and the Rictor-mammalian target of rapamycin complex (mTORC2), respectively. Serine 152-158 AKT serine/threonine kinase 1 Homo sapiens 99-102 18467333-5 2008 Additionally, the Pim kinases phosphorylate Mdm2 in vitro and in cultured cells at Ser(166) and Ser(186), two previously identified targets of other signaling pathways, including Akt. Serine 96-99 AKT serine/threonine kinase 1 Homo sapiens 179-182 18174290-8 2008 Knocking down DKK1 abrogated dexamethasone-modulated expression of nuclear beta-catenin and phosphorylated Ser(473)-Akt and survival of osteoblasts and adipocytic differentiation of mesenchymal progenitor cell cultures. Serine 107-110 AKT serine/threonine kinase 1 Homo sapiens 116-119 18519679-11 2008 Akt phosphorylation at Ser(473) was reduced by Mel-18 overexpression in SK-BR-3 cells and enhanced by Mel-18 suppression in T-47D cells. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 0-3 18321849-5 2008 These effects were accompanied by increased activity of the lipid phosphatase PTEN, decreased activity and phosphorylation (Ser(473)) of Akt, and induction of apoptosis. Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 137-140 18321849-6 2008 ONOO(-) enhanced Akt-Ser(473) phosphorylation in LKB1-deficient HeLa S3 cells or in HeLa S3 cells transfected with kinase-dead LKB1. Serine 21-24 AKT serine/threonine kinase 1 Homo sapiens 17-20 18321849-7 2008 Conversely, ONOO(-) inhibited Akt Ser(473) phosphorylation when wild type LKB1 were reintroduced in HeLa S3 cells. Serine 34-37 AKT serine/threonine kinase 1 Homo sapiens 30-33 18093597-8 2008 Analysis of hepatic Akt phosphorylation and mass revealed a small (25%) increase in serine phosphorylation of Akt with no significant change in Akt mass, although serine-phosphorylation and mass of Akt2 were significantly increased (+32%, p=0.03, and +42%, p=0.01, respectively). Serine 84-90 AKT serine/threonine kinase 1 Homo sapiens 20-23 18093597-8 2008 Analysis of hepatic Akt phosphorylation and mass revealed a small (25%) increase in serine phosphorylation of Akt with no significant change in Akt mass, although serine-phosphorylation and mass of Akt2 were significantly increased (+32%, p=0.03, and +42%, p=0.01, respectively). Serine 84-90 AKT serine/threonine kinase 1 Homo sapiens 110-113 18093597-8 2008 Analysis of hepatic Akt phosphorylation and mass revealed a small (25%) increase in serine phosphorylation of Akt with no significant change in Akt mass, although serine-phosphorylation and mass of Akt2 were significantly increased (+32%, p=0.03, and +42%, p=0.01, respectively). Serine 84-90 AKT serine/threonine kinase 1 Homo sapiens 110-113 18370992-8 2008 Phosphorylation at serine 473 of Akt was rescued in the presence high concentrations of insulin. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 33-36 18381446-7 2008 Furthermore, we show that inhibition of mTOR by rapamycin induces the activation of Akt as shown by increased Akt phosphorylation at Ser(473) and the inhibition of 6-TG-induced apoptosis and cell death. Serine 133-136 AKT serine/threonine kinase 1 Homo sapiens 84-87 18381446-7 2008 Furthermore, we show that inhibition of mTOR by rapamycin induces the activation of Akt as shown by increased Akt phosphorylation at Ser(473) and the inhibition of 6-TG-induced apoptosis and cell death. Serine 133-136 AKT serine/threonine kinase 1 Homo sapiens 110-113 18332867-5 2008 In mechanistic studies identifying AR degradation, isosilybin B caused increased phosphorylation of Akt (Ser-473 and Thr-308) and Mdm2 (Ser-166), which was linked with AR degradation as pretreatment with PI3K inhibitor (LY294002)-restored AR level. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 100-103 18064605-4 2008 DCT caused dose- and time-dependent Akt (Ser(473)) phosphorylation, a commonly used marker of activated PI3K/Akt signaling. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 36-39 18064605-4 2008 DCT caused dose- and time-dependent Akt (Ser(473)) phosphorylation, a commonly used marker of activated PI3K/Akt signaling. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 109-112 18064605-8 2008 DCT-induced PI3K/Akt activation resulted in downstream phosphorylation of GSK-3 (Ser(21/9)) and BAD (Ser(136)), and nuclear translocation (activation) of NF-kappaB, thereby confirming that DCT-induced activation of PI3K/Akt signaling regulates both proproliferative and prosurvival signals. Serine 81-84 AKT serine/threonine kinase 1 Homo sapiens 17-20 18064605-8 2008 DCT-induced PI3K/Akt activation resulted in downstream phosphorylation of GSK-3 (Ser(21/9)) and BAD (Ser(136)), and nuclear translocation (activation) of NF-kappaB, thereby confirming that DCT-induced activation of PI3K/Akt signaling regulates both proproliferative and prosurvival signals. Serine 101-104 AKT serine/threonine kinase 1 Homo sapiens 17-20 18370992-7 2008 Phosphorylation at serine 473 of the Akt protein (P-Akt), a protein representing a common pathway for insulin receptors (IR), insulinlike growth factor receptors (IGFR), and hybrid receptors formed by IR and IGFR subunits, was abolished in the presence of toxic doses of the phenylalkylamine CCB verapamil. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 37-40 18370992-7 2008 Phosphorylation at serine 473 of the Akt protein (P-Akt), a protein representing a common pathway for insulin receptors (IR), insulinlike growth factor receptors (IGFR), and hybrid receptors formed by IR and IGFR subunits, was abolished in the presence of toxic doses of the phenylalkylamine CCB verapamil. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 52-55 18350142-2 2008 MAPK and Akt have been shown to phosphorylate the estrogen receptor (ER) at Ser-118 and Ser-167, respectively, thereby mimicking the effects of estrogenic activity such as estrogen responsive element (ERE)-dependent transcription. Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 9-12 18261725-4 2008 Rapamycin pretreatment resulted in a decrease (20-30%) in the insulin mediated phosphorylation of Akt1 (Ser 473) in parental HepG2 cells but showed an upregulation of phosphorylation in HepG2-CA-Akt/PKB cells. Serine 104-107 AKT serine/threonine kinase 1 Homo sapiens 98-102 18261725-4 2008 Rapamycin pretreatment resulted in a decrease (20-30%) in the insulin mediated phosphorylation of Akt1 (Ser 473) in parental HepG2 cells but showed an upregulation of phosphorylation in HepG2-CA-Akt/PKB cells. Serine 104-107 AKT serine/threonine kinase 1 Homo sapiens 98-101 18261725-6 2008 Furthermore, rictor knockdown decreased the phosphorylation of Akt (Ser 473) by 40-60% upon rapamycin pretreatment. Serine 68-71 AKT serine/threonine kinase 1 Homo sapiens 63-66 18261725-8 2008 The changes in the levels of phosphorylated Akt/PKB (Ser 473) correlated with GS and protein phoshatase-1 activity. Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 44-47 18261725-8 2008 The changes in the levels of phosphorylated Akt/PKB (Ser 473) correlated with GS and protein phoshatase-1 activity. Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 48-51 17721885-3 2008 IRS-1 knockdown in both cell lines resulted in reduction of insulin stimulated Akt1 phosphorylation at Ser 473. Serine 103-106 AKT serine/threonine kinase 1 Homo sapiens 79-83 18350142-2 2008 MAPK and Akt have been shown to phosphorylate the estrogen receptor (ER) at Ser-118 and Ser-167, respectively, thereby mimicking the effects of estrogenic activity such as estrogen responsive element (ERE)-dependent transcription. Serine 88-91 AKT serine/threonine kinase 1 Homo sapiens 9-12 18242595-1 2008 Previously, we demonstrated that hepatocyte growth factor (HGF) potently stimulates endothelial nitric oxide synthase (eNOS) activity and nitric oxide (NO) production through a calcium- and Akt-mediated phosphorylation at Ser-1179 (Ser-1177 human) in bovine aortic endothelial cells. Serine 232-235 AKT serine/threonine kinase 1 Homo sapiens 190-193 18191107-5 2008 TGF-beta1 stimulation increased the phosphorylation of p85 subunit of PI3K, and serine 473 of Akt. Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 94-97 18191107-8 2008 The TGF-beta1-mediated increases in IKKalpha/beta phosphorylation and p65 Ser(536) phosphorylation were inhibited by Ly294002 and Akt inhibitor. Serine 74-77 AKT serine/threonine kinase 1 Homo sapiens 130-133 18242595-1 2008 Previously, we demonstrated that hepatocyte growth factor (HGF) potently stimulates endothelial nitric oxide synthase (eNOS) activity and nitric oxide (NO) production through a calcium- and Akt-mediated phosphorylation at Ser-1179 (Ser-1177 human) in bovine aortic endothelial cells. Serine 222-225 AKT serine/threonine kinase 1 Homo sapiens 190-193 18256541-5 2008 The following manuscript demonstrates that Akt can directly associate with Raf-1 causing its inactivation via phosphorylation of a negative regulatory residue (serine 259). Serine 160-166 AKT serine/threonine kinase 1 Homo sapiens 43-46 18339839-6 2008 Depletion of ILK and rictor in breast and prostate cancer cell lines resulted in inhibition of Akt Ser(473) phosphorylation and induction of apoptosis, whereas, in several cell lines, depletion of mTOR increased Akt phosphorylation. Serine 99-102 AKT serine/threonine kinase 1 Homo sapiens 95-98 18339839-7 2008 Akt and Ser(473)P-Akt were detected in ILK immunoprecipitates and small interfering RNA-mediated depletion of rictor, but not mTOR, inhibited the amount of Ser(473)P-Akt in the ILK complex. Serine 8-11 AKT serine/threonine kinase 1 Homo sapiens 18-21 18339839-7 2008 Akt and Ser(473)P-Akt were detected in ILK immunoprecipitates and small interfering RNA-mediated depletion of rictor, but not mTOR, inhibited the amount of Ser(473)P-Akt in the ILK complex. Serine 8-11 AKT serine/threonine kinase 1 Homo sapiens 18-21 18339839-7 2008 Akt and Ser(473)P-Akt were detected in ILK immunoprecipitates and small interfering RNA-mediated depletion of rictor, but not mTOR, inhibited the amount of Ser(473)P-Akt in the ILK complex. Serine 156-159 AKT serine/threonine kinase 1 Homo sapiens 0-3 18339839-8 2008 Expression of the NH(2)-terminal (1-398 amino acids) rictor domain also resulted in the inhibition of ILK-associated Akt Ser(473) phosphorylation. Serine 121-124 AKT serine/threonine kinase 1 Homo sapiens 117-120 17983605-4 2008 Western blotting showed that total Akt protein did not change in hibernation but active, phosphorylated Akt (Ser 473) was reduced by 40% in muscle compared with euthermic controls and was almost undetectable in liver. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 104-107 18160431-6 2008 Hepatocytes infected with cell culture-grown HCV genotype 1a or 2a displayed a significant increase in the Ser(473) phosphorylation status of the Ser/Thr kinase protein kinase B (Akt/PKB), while Thr(308) phosphorylation was not significantly altered. Serine 107-110 AKT serine/threonine kinase 1 Homo sapiens 179-182 17961125-2 2008 PIF induced transient phosphorylation of Akt at Ser(473) within 30 min, which was attenuated by the PI3K (phosphoinositide 3-kinase) inhibitor LY294002 and the tyrosine kinase inhibitor genistein. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 41-44 18787612-2 2008 Downstream signaling from PI3"-K/Akt leads to phosphorylation (p) of mTOR at serine 2448 and to activation of its substrate, p70S6Kinase (p70S6K), phosphorylated on threonine 389. Serine 77-83 AKT serine/threonine kinase 1 Homo sapiens 33-36 18068336-13 2008 Downregulation of rictor inhibited serine 473 phosphorylation of Akt without any effect on phosphorylation of its substrate, tuberin. Serine 35-41 AKT serine/threonine kinase 1 Homo sapiens 65-68 17941081-6 2008 In the physiologically hypertrophied heart after exercise training, total Akt protein level was unchanged, but Akt was chronically hyperphosphorylated at serine 473. Serine 154-160 AKT serine/threonine kinase 1 Homo sapiens 111-114 17724478-6 2008 Furthermore, we found that while ARC inhibited the phosphorylation of Akt Ser-473 in multiple cancer cell lines, forced expression of myristoylated Akt promoted resistance to ARC-induced apoptosis in neuroblastoma cells. Serine 74-77 AKT serine/threonine kinase 1 Homo sapiens 70-73 17906677-6 2008 In addition, Ang II diminished insulin-stimulated phosphorylation of Akt (at Ser(473)) and eNOS (at Ser(1177)) and NO generation, effects which were reversed by ACE2 gene transfer and anti-MIF treatment in endothelial cells. Serine 77-80 AKT serine/threonine kinase 1 Homo sapiens 69-72 18441516-6 2008 Insulin stimulation increased the activity of IR-kinase, IRS-1 phosphorylation, p85 association with IRS-1 and Ser(273)-phosphorylation of Akt by at least 250%, respectively and decreased the C3-activity by 45% (p < 0.01, respectively). Serine 111-114 AKT serine/threonine kinase 1 Homo sapiens 139-142 17993260-8 2008 A two-threefold increase in Akt Thr-308 and Akt Ser-473 protein kinase activities and their phosphoprotein levels was observed in TCL1 immunoprecipitates of plasma membranes and nuclei of the treated cells. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 28-31 17993260-8 2008 A two-threefold increase in Akt Thr-308 and Akt Ser-473 protein kinase activities and their phosphoprotein levels was observed in TCL1 immunoprecipitates of plasma membranes and nuclei of the treated cells. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 44-47 18409144-2 2008 Akt is activated by phosphorylation on two critical residues, namely threonine 308 (Thr308) and serine 473 (Ser473). Serine 96-102 AKT serine/threonine kinase 1 Homo sapiens 0-3 19011679-9 2008 In addition LBW had reduced insulin stimulated phospho-Akt (Ser 473) (p<0.01), indicative of reduced Akt signalling. Serine 60-63 AKT serine/threonine kinase 1 Homo sapiens 55-58 19240370-10 2008 Additionally, synergistic phosphorylation of Akt (Ser-473) and phospho-ERK1+ERK2 (Thr202/Tyr204) was also seen indicating that EGF and HGF could induce synergistic phosphorylation of important signaling intermediates. Serine 50-53 AKT serine/threonine kinase 1 Homo sapiens 45-48 18199958-11 2008 Immunohistochemical analysis of 14 VS tissue sections detected positive staining for activated AKT phosphorylated at both serine-473 and threonine-308 in all tumors. Serine 122-128 AKT serine/threonine kinase 1 Homo sapiens 95-98 18059161-12 2007 Moreover EGCG and GTP decreased AKT phosphorylation as found out by Western blotting for Phospho-AKT (Ser-473). Serine 102-105 AKT serine/threonine kinase 1 Homo sapiens 32-35 17951252-6 2007 Inhibitory Ser-9 phosphorylation of glycogen synthase kinase 3beta (GSK3beta) by Akt prevented proteasome-mediated cyclin D1 degradation and induced cell cycle progress in LeTx-intoxicated THP-1 cells. Serine 11-14 AKT serine/threonine kinase 1 Homo sapiens 81-84 18059161-12 2007 Moreover EGCG and GTP decreased AKT phosphorylation as found out by Western blotting for Phospho-AKT (Ser-473). Serine 102-105 AKT serine/threonine kinase 1 Homo sapiens 97-100 17961557-4 2007 More importantly, we demonstrate that TGFbeta significantly abolished Akt-mediated serine-9 phosphorylation of glycogen synthase kinase 3beta (GSK3beta), thus prevented its inactivation. Serine 83-89 AKT serine/threonine kinase 1 Homo sapiens 70-73 17974987-8 2007 VX-680, a selective Aurora kinase inhibitor, decreased Akt1 phosphorylation at Ser(473) and inhibited cell migration, but failed to do so in constitutive active Akt1 (myr-Akt1)-overexpressed cells. Serine 79-82 AKT serine/threonine kinase 1 Homo sapiens 55-59 17458623-4 2007 The cytoplasmic/nuclear localization of tuberin is known to be regulated by the serine/threonine protein kinase Akt. Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 112-115 17615157-2 2007 In contrast to a previous study, we found CTMP overexpression to significantly enhance Akt phosphorylation at both Thr(308) and Ser(473) as well as the kinase activity of Akt, while phosphatidylinositol 3-kinase (PI3-kinase) activity was unaffected. Serine 128-131 AKT serine/threonine kinase 1 Homo sapiens 87-90 17720765-6 2007 Akt kinase, which can phosphorylate eNOS Ser(1177), was also decreased by hypoxia, regarding both total protein content and the phosphorylated (active) form. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 0-3 17715136-7 2007 Furthermore, mutation of consensus phosphorylation sites on Nedd4-2 for Akt1 and Sgk1, Ser(342) and Ser(428), completely abolished the inhibitory effect of Sgk1 and Akt1 on Nedd4-2 action. Serine 87-90 AKT serine/threonine kinase 1 Homo sapiens 165-169 17888676-6 2007 Akt co-localizes with IIGP165 protein in the Golgi in vivo, and phosphorylates IIGP165 on serine residues 345 and 134. Serine 90-96 AKT serine/threonine kinase 1 Homo sapiens 0-3 17715136-7 2007 Furthermore, mutation of consensus phosphorylation sites on Nedd4-2 for Akt1 and Sgk1, Ser(342) and Ser(428), completely abolished the inhibitory effect of Sgk1 and Akt1 on Nedd4-2 action. Serine 100-103 AKT serine/threonine kinase 1 Homo sapiens 165-169 17684102-6 2007 After the 12- but not the 48-h fast, insulin-stimulated AKT Ser(473) phosphorylation was greater in lean than obese subjects. Serine 60-63 AKT serine/threonine kinase 1 Homo sapiens 56-59 17640974-6 2007 PKA inhibitor H89 is able to decrease threonine phosphorylation of Akt and to increase serine phosphorylation of Akt by p38 MAPK and counteracts the cytoprotective effect induced by PGE2. Serine 87-93 AKT serine/threonine kinase 1 Homo sapiens 113-116 17640974-8 2007 The effect of PGE2 on Caco-2 cell survival through PKA activation is mediated and regulated by the balance of threonine/serine phosphorylation of Akt by p38 kinase and PI3K. Serine 120-126 AKT serine/threonine kinase 1 Homo sapiens 146-149 17908967-2 2007 Subsequent studies have shown that Ser(167) phosphorylation is important in the regulation of ER activity and have identified p90RSK and AKT as protein kinases that phosphorylate Ser(167). Serine 35-38 AKT serine/threonine kinase 1 Homo sapiens 137-140 17908967-2 2007 Subsequent studies have shown that Ser(167) phosphorylation is important in the regulation of ER activity and have identified p90RSK and AKT as protein kinases that phosphorylate Ser(167). Serine 179-182 AKT serine/threonine kinase 1 Homo sapiens 137-140 17595317-6 2007 Serine-9 on GSK3 can be phosphorylated by Sgk (serum- and glucocorticoid-induced protein kinase) and by Akt. Serine 0-6 AKT serine/threonine kinase 1 Homo sapiens 104-107 17640984-4 2007 Sequence analysis surrounding Ser(629) revealed conformity to the consensus phosphorylation sequence recognized by Akt. Serine 30-33 AKT serine/threonine kinase 1 Homo sapiens 115-118 17640984-6 2007 In addition, Ser(629) of IRS-1 is directly phosphorylated by Akt in vitro. Serine 13-16 AKT serine/threonine kinase 1 Homo sapiens 61-64 17640984-9 2007 Finally, in vitro phosphorylation of a Ser(629)-containing IRS-1 fragment with Akt reduces the subsequent ability of ERK to phosphorylate Ser(636/639). Serine 39-42 AKT serine/threonine kinase 1 Homo sapiens 79-82 17640984-9 2007 Finally, in vitro phosphorylation of a Ser(629)-containing IRS-1 fragment with Akt reduces the subsequent ability of ERK to phosphorylate Ser(636/639). Serine 138-141 AKT serine/threonine kinase 1 Homo sapiens 79-82 17640984-10 2007 These results suggest that a feed-forward mechanism may exist whereby insulin activation of Akt leads to phosphorylation of IRS-1 at Ser(629), resulting in decreased phosphorylation of IRS-1 at Ser(636) and enhanced downstream signaling. Serine 133-136 AKT serine/threonine kinase 1 Homo sapiens 92-95 17640984-10 2007 These results suggest that a feed-forward mechanism may exist whereby insulin activation of Akt leads to phosphorylation of IRS-1 at Ser(629), resulting in decreased phosphorylation of IRS-1 at Ser(636) and enhanced downstream signaling. Serine 194-197 AKT serine/threonine kinase 1 Homo sapiens 92-95 17696989-5 2007 The protective effect of reelin includes activating the Src-family kinases/PI3 K/Akt pathway which then led to selective phosphorylation of Bcl-2/Bcl-XL associated death promoter (BAD) at serine-136, while the phosphorylation-incompetent mutation of BAD (S136A) suppressed this protection. Serine 188-194 AKT serine/threonine kinase 1 Homo sapiens 81-84 17646428-5 2007 These metastatic AGS-GFPM1/2 cells expressed a higher level of phosphorylated serine 473 on AKT (p-AKT). Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 92-95 17646428-5 2007 These metastatic AGS-GFPM1/2 cells expressed a higher level of phosphorylated serine 473 on AKT (p-AKT). Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 97-102 17566113-7 2007 LA"s antiapoptotic activity was reduced by PI 3-kinase inhibitors (wortmannin, LY-294002), being in line with LA-induced Akt phosphorylation (Ser(437), +159 +/- 43%; Thr(308), +98 +/- 25%; P < 0.01). Serine 142-145 AKT serine/threonine kinase 1 Homo sapiens 121-124 17891137-4 2007 Here, we show that the protein kinase Akt directly binds to and phosphorylates merlin on residues Thr 230 and Ser 315, which abolishes merlin NTD/CTD interactions and binding to merlin"s effector protein PIKE-L and other binding partners. Serine 110-113 AKT serine/threonine kinase 1 Homo sapiens 38-41 17684018-7 2007 Suppression of PTEN expression with siRNA significantly reduced the p53 and p21 levels and activated Akt phosphorylation at Ser(473) and Thr(308), resulting in decreased apoptosis and increased cell survival. Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 101-104 17562705-7 2007 Treatment of cells with 401 blocked the phosphorylation of sites modified by mTOR-Raptor and mTOR-Rictor complexes (ribosomal protein S6 kinase 1 Thr(389) and Akt Ser(473), respectively). Serine 163-166 AKT serine/threonine kinase 1 Homo sapiens 159-162 17634416-10 2007 In an in vitro kinase assay, active recombinant ERK phosphorylated recombinant Akt on Ser(473). Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 79-82 17634416-13 2007 These findings suggest that ouabain-stimulated ERK phosphorylation is required for Akt phosphorylation on Ser(473), cell proliferation, and stimulation of Na(+)/K(+)-ATPase-mediated (86)Rb uptake in OK cells. Serine 106-109 AKT serine/threonine kinase 1 Homo sapiens 83-86 18163231-6 2007 Here we demonstrate that Akt phosphorylates Cx43 on Ser373 and Ser369; antibodies recognizing Akt-phosphorylated sites or phospho-Ser "mode-1" 14-3-3-binding sites recognize a protein from EGF-treated cells that migrates as Cx43, and GST-14-3-3 binds to Cx43 phosphorylated endogenously in EGF-treated cells. Serine 52-55 AKT serine/threonine kinase 1 Homo sapiens 25-28 17572661-5 2007 Akt binds and phosphorylates zyxin on serine 142, leading to its association with acinus. Serine 38-44 AKT serine/threonine kinase 1 Homo sapiens 0-3 17512084-5 2007 Phosphorylation of the key cell survival kinase, Akt, was significantly increased, resulting in increased serine phosphorylation of the downstream target, GSK3-beta. Serine 106-112 AKT serine/threonine kinase 1 Homo sapiens 49-52 17540008-3 2007 Previous work demonstrated that phosphorylation of mutant ATXN1 at serine 776 (S776), a putative Akt phosphorylation site, is critical for pathogenesis. Serine 67-73 AKT serine/threonine kinase 1 Homo sapiens 97-100 17334390-0 2007 Akt inhibitor A-443654 induces rapid Akt Ser-473 phosphorylation independent of mTORC1 inhibition. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 0-3 17334390-0 2007 Akt inhibitor A-443654 induces rapid Akt Ser-473 phosphorylation independent of mTORC1 inhibition. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 37-40 17334390-1 2007 Rapamycin, a natural product inhibitor of the Raptor-mammalian target of rapamycin complex (mTORC1), is known to induce Protein kinase B (Akt/PKB) Ser-473 phosphorylation in a subset of human cancer cell lines through inactivation of S6K1, stabilization of insulin receptor substrate (IRS)-1, and increased signaling through the insulin/insulin-like growth factor-I/phosphatidylinositol 3-kinase (PI3K) axis. Serine 147-150 AKT serine/threonine kinase 1 Homo sapiens 138-145 17334390-2 2007 We report that A-443654, a potent small-molecule inhibitor of Akt serine/threonine kinases, induces Akt Ser-473 phosphorylation in all human cancer cell lines tested, including PTEN- and TSC2-deficient lines. Serine 104-107 AKT serine/threonine kinase 1 Homo sapiens 100-103 17334390-6 2007 Small interfering RNA-mediated knockdown of mTOR or Rictor, components of the rapamycin-insensitive mTORC2 complex, but not the mTORC1 component Raptor, also inhibited Akt Ser-473 phosphorylation induced by A-443654. Serine 172-175 AKT serine/threonine kinase 1 Homo sapiens 168-171 17535800-3 2007 We report here that POSH is a direct substrate for phosphorylation by Akt in vivo and in vitro, and we identify a major site of Akt phosphorylation as serine 304 of POSH, which lies within the Rac-binding domain. Serine 151-157 AKT serine/threonine kinase 1 Homo sapiens 70-73 17665439-10 2007 JNK, ERK-1/2, and Akt (at both Ser-473 and Thr-308) were each up-regulated in a time-dependent manner. Serine 31-34 AKT serine/threonine kinase 1 Homo sapiens 18-21 17565979-10 2007 mTORC2 has been described as the catalytic complex that phosphorylates AKT/PKB at Ser-473 and elicits activation of Rho GTPases and cytoskeletal reorganization. Serine 82-85 AKT serine/threonine kinase 1 Homo sapiens 75-78 17505062-10 2007 BRCA1 knockdown caused an increase in ER-alpha phosphorylation on serine-167 (but not serine-118 or serine-104/106) that was dependent on PI3K/Akt signaling and was mimicked by pharmacologic inhibition of protein phosphatase 2A. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 143-146 17505062-11 2007 These findings suggest that BRCA1 regulates Akt signaling and the PI3K/Akt pathway modulates the ability of BRCA1 to repress ER-alpha, in part through serine phosphorylation events in the activation function-1 domain of ER-alpha. Serine 151-157 AKT serine/threonine kinase 1 Homo sapiens 44-47 17505062-11 2007 These findings suggest that BRCA1 regulates Akt signaling and the PI3K/Akt pathway modulates the ability of BRCA1 to repress ER-alpha, in part through serine phosphorylation events in the activation function-1 domain of ER-alpha. Serine 151-157 AKT serine/threonine kinase 1 Homo sapiens 71-74 17535800-3 2007 We report here that POSH is a direct substrate for phosphorylation by Akt in vivo and in vitro, and we identify a major site of Akt phosphorylation as serine 304 of POSH, which lies within the Rac-binding domain. Serine 151-157 AKT serine/threonine kinase 1 Homo sapiens 128-131 17848732-7 2007 Western blots indicated increased (P < 0.05) Akt (Ser 473) phosphorylation in skeletal muscle from animals refed ZS diets for 3 and 6 h as compared to F animals. Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 48-51 17652589-3 2007 Ca2+ supplied through Ca2+-permeable AMPA receptor phosphorylated Akt at Ser-473, thereby facilitating proliferation and mobility. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 66-69 17556533-6 2007 BMOV also induced the phosphorylation of Thr(308) and Ser(473) of Akt in both cell lines receiving insulin post-treatment, while dexamethasone decreased those phosphorylations. Serine 54-57 AKT serine/threonine kinase 1 Homo sapiens 66-69 17922342-7 2007 CSB treatment also decreased the level of phospho-Ser(473)-Akt (p < .05 versus control). Serine 50-53 AKT serine/threonine kinase 1 Homo sapiens 59-62 17922342-8 2007 Inhibition of PKA by H-89 did not affect CSB-induced phosphorylation, whereas the PKB inhibitor LY-294002 enhanced it at Ser(1117). Serine 121-124 AKT serine/threonine kinase 1 Homo sapiens 82-85 17515613-3 2007 Here we identify an Akt consensus phosphorylation motif in the actin-based motor protein myosin 5a and show that insulin stimulation leads to phosphorylation of myosin 5a at serine 1650. Serine 174-180 AKT serine/threonine kinase 1 Homo sapiens 20-23 17548608-10 2007 Furthermore, the defective Akt phosphorylation in these cells was specific for the Ser(473) but not the Thr(308) phosphorylation site of this molecule. Serine 83-86 AKT serine/threonine kinase 1 Homo sapiens 27-30 17339610-3 2007 Akt activity is stimulated by fMLP through phosphorylation on threonine 308 (Thr308) and serine 473 (Ser473) by 3-phosphoinositide-dependent kinase 1 (PDK1) and MAPK-AP kinase (APK)-APK-2 (MAPKAPK-2), respectively, in a PI-3K-dependent manner. Serine 89-95 AKT serine/threonine kinase 1 Homo sapiens 0-3 17549359-5 2007 Using tissue microarray technology, immunohistochemical expression of phosphorylated Akt (pAkt) at Ser-473 was evaluated in 127 cases of invasive ductal carcinomas, together with hormone receptors, HER2, p53, Ki-67 and other clinicopathological variables. Serine 99-102 AKT serine/threonine kinase 1 Homo sapiens 85-88 17545609-5 2007 For Akt to be fully activated, Ser(473) on its COOH terminus needs to be phosphorylated. Serine 31-34 AKT serine/threonine kinase 1 Homo sapiens 4-7 17545609-6 2007 We detected more Ser(473) Akt phosphorylation in MT cells, derived from a pleural effusion, compared with cells from the primary tumor. Serine 17-20 AKT serine/threonine kinase 1 Homo sapiens 26-29 17545609-8 2007 By using genetic and pharmacologic activators and inhibitors, we showed that Ser(473) Akt phosphorylation is more sensitive to mTOR/rictor inhibition in metastatic tumor cells than normal mammary epithelial and primary tumor cells. Serine 77-80 AKT serine/threonine kinase 1 Homo sapiens 86-89 17545609-9 2007 The mTOR/rictor kinase activity was indispensable for both Ser(473) Akt phosphorylation and migration of metastatic MT2 cells. Serine 59-62 AKT serine/threonine kinase 1 Homo sapiens 68-71 17545609-10 2007 In addition, a large decrease of protein phosphatase PH domain leucine-rich repeat protein phosphatase (PHLPP) was found, which could be responsible for the overexpression of Ser(473) Akt in MT cells. Serine 175-178 AKT serine/threonine kinase 1 Homo sapiens 184-187 17276500-13 2007 The PI3K/Akt pathway inhibitor wortmannin decreased the amount of ser-80 MKK4 by 50%, and inhibited EGF stimulation of MKK4 ser-80 phosphorylation by 60%. Serine 66-69 AKT serine/threonine kinase 1 Homo sapiens 9-12 17332141-6 2007 The erbB4 receptor ligand neuregulin-1beta (NRG1beta) promoted erbB2/4 tryosine phosphorylation and Akt serine phosphorylation in ventricular myocytes, whereas treatment with ET-1 did not. Serine 104-110 AKT serine/threonine kinase 1 Homo sapiens 100-103 17400190-5 2007 It was demonstrated that activation of phosphatidylinositol 3-kinase (PI3K) and Akt1 facilitated the nuclear translocation of Akt1, but the phosphorylation at threonine 308 and serine 473 was not prerequisite. Serine 177-183 AKT serine/threonine kinase 1 Homo sapiens 80-84 17276500-13 2007 The PI3K/Akt pathway inhibitor wortmannin decreased the amount of ser-80 MKK4 by 50%, and inhibited EGF stimulation of MKK4 ser-80 phosphorylation by 60%. Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 9-12 17336927-1 2007 Previously we identified an unusual potential dual Akt/protein kinase B consensus phosphorylation motif in the protein Synip (RxKxRS(97)xS(99)) with serine 99 as a unique Akt2, but not Akt1 or for Akt3, substrate phosphorylation site. Serine 149-155 AKT serine/threonine kinase 1 Homo sapiens 51-54 17255531-7 2007 The action of CK2 and AKT on STAT1 activation was mediated, at least in part, through the regulation of serine 727 phosphorylation. Serine 104-110 AKT serine/threonine kinase 1 Homo sapiens 22-25 17311912-0 2007 Akt attenuation of the serine protease activity of HtrA2/Omi through phosphorylation of serine 212. Serine 23-29 AKT serine/threonine kinase 1 Homo sapiens 0-3 17311912-4 2007 Here we report that serine/threonine kinases Akt1 and Akt2 phosphorylate mitochondria-released HtrA2/Omi on serine 212 in vivo and in vitro, which results in attenuation of its serine protease activity and pro-apoptotic function. Serine 20-26 AKT serine/threonine kinase 1 Homo sapiens 45-49 17440093-9 2007 Finally, immunofluorescence staining revealed elevated phosphorylated Akt (Ser(473)) and phosphorylated mammalian target of rapamycin (mTOR; Ser(2448)) in human gallbladder cancer compared with normal gallbladder tissue. Serine 75-78 AKT serine/threonine kinase 1 Homo sapiens 70-73 17332930-8 2007 Furthermore, curcumin inhibited expression of phosphatidyl-inositol-3 kinase (PI3K) p110 and p85 subunits, and phosphorylation of Ser 473 AKT/PKB. Serine 130-133 AKT serine/threonine kinase 1 Homo sapiens 138-141 17166829-6 2007 Mutation of serine 255, an Akt consensus site contained in the hinge region of ERbeta, prevented the release of CBP and rendered ERbeta transcriptionally more responsive to CBP coactivation, suggesting that Ser-255 may serve as a regulatory site to restrain ERbeta activity in Akt-activated cells. Serine 12-18 AKT serine/threonine kinase 1 Homo sapiens 27-30 17062729-4 2007 CD4+CD25+ Treg cells displayed a consistent defect in phosphorylation of AKT at serine 473 and reduced phosphorylation of the AKT substrates FOXO and S6. Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 73-76 17200115-7 2007 By using overexpression and RNA interference, we show here that IEX-1 increases Akt/PKB activity in response to various growth factors by preventing Akt dephosphorylation on both Thr(308) and Ser(473) residues. Serine 192-195 AKT serine/threonine kinase 1 Homo sapiens 80-87 17166829-6 2007 Mutation of serine 255, an Akt consensus site contained in the hinge region of ERbeta, prevented the release of CBP and rendered ERbeta transcriptionally more responsive to CBP coactivation, suggesting that Ser-255 may serve as a regulatory site to restrain ERbeta activity in Akt-activated cells. Serine 12-18 AKT serine/threonine kinase 1 Homo sapiens 277-280 17166829-6 2007 Mutation of serine 255, an Akt consensus site contained in the hinge region of ERbeta, prevented the release of CBP and rendered ERbeta transcriptionally more responsive to CBP coactivation, suggesting that Ser-255 may serve as a regulatory site to restrain ERbeta activity in Akt-activated cells. Serine 207-210 AKT serine/threonine kinase 1 Homo sapiens 27-30 17166829-6 2007 Mutation of serine 255, an Akt consensus site contained in the hinge region of ERbeta, prevented the release of CBP and rendered ERbeta transcriptionally more responsive to CBP coactivation, suggesting that Ser-255 may serve as a regulatory site to restrain ERbeta activity in Akt-activated cells. Serine 207-210 AKT serine/threonine kinase 1 Homo sapiens 277-280 17107963-6 2007 In contrast, Ser-166 phosphorylation of Mdm2 in lung cells was mediated by Akt. Serine 13-16 AKT serine/threonine kinase 1 Homo sapiens 75-78 17263796-4 2007 Exogenous galanin stimulates the phosphorylation of the serine/threonine kinase Akt and extracellular signal-regulated kinase (ERK) in wild-type (WT) cultures by 435 +/- 5% and 278 +/- 2%, respectively. Serine 56-62 AKT serine/threonine kinase 1 Homo sapiens 80-83 17309805-12 2007 Moreover, following infection with baculovirus, SHSY-5Y and C3A cells respond to insulin by means of phosphorylation of Akt on serine-473 in the same manner as uninfected cells. Serine 127-133 AKT serine/threonine kinase 1 Homo sapiens 120-123 17107963-10 2007 Furthermore, we have shown that myr-Akt-induced overexpression of pAkt suppressed the levels of pMdm2 Ser-166 in hepatocytes. Serine 102-105 AKT serine/threonine kinase 1 Homo sapiens 36-39 16545819-4 2006 In Western blot analysis, cilostazol increased phosphorylation of endothelial nitric oxide synthase (eNOS) at Ser(1177) and of Akt at Ser(473) and dephosphorylation of eNOS at Thr(495). Serine 134-137 AKT serine/threonine kinase 1 Homo sapiens 127-130 17822324-4 2007 Mouse monoclonal antibodies against GFAP, PTEN, PKB/Akt phosphorylated on serine 473, EGFR, p53, p21Waf1/Cip1 and MDM2 were used, followed by standard indirect immunohistochemical method. Serine 74-80 AKT serine/threonine kinase 1 Homo sapiens 52-55 17234785-3 2007 Here, we provide for the first time evidence that phosphorylation of Akt at serine 473 (S473) and/or threonine 308 (T308), S6 ribosomal protein, and ERK frequently occurs in primary neuroblastoma. Serine 76-82 AKT serine/threonine kinase 1 Homo sapiens 69-72 17237292-7 2007 Both total Akt protein and active phosphorylated levels of Akt (Ser(473)) and phosphoinositide 3-kinase were significantly decreased in sulforaphane-treated SKOV3, C3, and T3 cells with a concomitant inhibition of Akt kinase activity by sulforaphane in SKOV3 and C3 cells. Serine 64-67 AKT serine/threonine kinase 1 Homo sapiens 59-62 17237292-7 2007 Both total Akt protein and active phosphorylated levels of Akt (Ser(473)) and phosphoinositide 3-kinase were significantly decreased in sulforaphane-treated SKOV3, C3, and T3 cells with a concomitant inhibition of Akt kinase activity by sulforaphane in SKOV3 and C3 cells. Serine 64-67 AKT serine/threonine kinase 1 Homo sapiens 59-62 17178855-6 2006 Conversely, forced HMGA1 overexpression resulted in significant increases in cellular invasiveness; in cellular MMP-9 activity, mRNA levels, and promoter activity; and in Akt phosphorylation at Ser(473). Serine 194-197 AKT serine/threonine kinase 1 Homo sapiens 171-174 17013611-9 2006 Immunohistochemistry with antibodies against phosphorylated Akt (phospho-Akt; Ser 473) in FCD(IIb) (n=23) showed strong phospho-Akt expression in dysplastic FCD(IIb) components. Serine 78-81 AKT serine/threonine kinase 1 Homo sapiens 60-63 17013611-9 2006 Immunohistochemistry with antibodies against phosphorylated Akt (phospho-Akt; Ser 473) in FCD(IIb) (n=23) showed strong phospho-Akt expression in dysplastic FCD(IIb) components. Serine 78-81 AKT serine/threonine kinase 1 Homo sapiens 73-76 17013611-9 2006 Immunohistochemistry with antibodies against phosphorylated Akt (phospho-Akt; Ser 473) in FCD(IIb) (n=23) showed strong phospho-Akt expression in dysplastic FCD(IIb) components. Serine 78-81 AKT serine/threonine kinase 1 Homo sapiens 73-76 16940331-6 2006 PMA induced the phosphorylation of Akt at Ser-473 and Thr-308 and the phosphorylation of Akt substrates, independently of PI-3K in B-CLL cells. Serine 42-45 AKT serine/threonine kinase 1 Homo sapiens 35-38 17077177-10 2006 Ser-244 phosphorylation, which is a putative Akt phosphorylation site, on PAK3 but not on PAK1 also regulates the morphological polarization of activated T cells by AILIM/ICOS signaling. Serine 0-3 AKT serine/threonine kinase 1 Homo sapiens 45-48 17058267-4 2006 Our results showed that a KD did not affect the expression levels of Akt, Bad, Bcl-xL, Bax, and 14-3-3 but increased phospho-Akt [serine 473; p-Akt (Ser473)] and phospho-Bad [serine 136; p-Bad (Ser136)] expression levels as well as decreased cleaved caspase-3 levels following a KA-induced seizure in the hippocampus. Serine 130-136 AKT serine/threonine kinase 1 Homo sapiens 125-128 17058267-4 2006 Our results showed that a KD did not affect the expression levels of Akt, Bad, Bcl-xL, Bax, and 14-3-3 but increased phospho-Akt [serine 473; p-Akt (Ser473)] and phospho-Bad [serine 136; p-Bad (Ser136)] expression levels as well as decreased cleaved caspase-3 levels following a KA-induced seizure in the hippocampus. Serine 130-136 AKT serine/threonine kinase 1 Homo sapiens 125-128 17041888-11 2006 RESULTS: Serum-stimulated phosphorylation of AKT1 was inhibited by SH-6 at doses > or =10 microM (>70% decrease in Threonine 308 and Serine 473 phosphorylation of AKT1). Serine 139-145 AKT serine/threonine kinase 1 Homo sapiens 45-49 17177604-3 2006 AKT phosphorylates the mRNA decay-promoting factor KSRP at a unique serine residue, induces its association with the multifunctional protein 14-3-3, and prevents KSRP interaction with the exoribonucleolytic complex exosome. Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 0-3 17308356-5 2006 Generally, the level of Akt phosphorylation at site Ser 473 (P-Akt) transiently increases after focal ischemia, whereas the levels of phosphorylation of PTEN, PDK1, forkhead transcription factor, and GSK3beta decrease. Serine 52-55 AKT serine/threonine kinase 1 Homo sapiens 24-27 17308356-5 2006 Generally, the level of Akt phosphorylation at site Ser 473 (P-Akt) transiently increases after focal ischemia, whereas the levels of phosphorylation of PTEN, PDK1, forkhead transcription factor, and GSK3beta decrease. Serine 52-55 AKT serine/threonine kinase 1 Homo sapiens 63-66 17016640-5 2006 Moreover, by Western blotting, IKKbeta was found to be able to upregulate Akt phosphorylation on Ser 473. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 74-77 16873402-7 2006 beta(2)AR activation also increased NOS activity and phosphorylation of NOS-3 on serine-1177, and these increases were attenuated by inhibition of protein kinase A (PKA), phosphatidylinositol 3-kinase (PI3K) or Akt, and abolished by coinhibition of PKA and Akt. Serine 81-87 AKT serine/threonine kinase 1 Homo sapiens 211-214 16926151-5 2006 Using newly developed phosphospecific antibodies to p300 (pSer(1834)), and site-directed mutant proteins, we find that suberoylanilide hydroxamic acid stimulates Akt activity, which is required to phosphorylate p300 at Ser(1834). Serine 59-62 AKT serine/threonine kinase 1 Homo sapiens 162-165 16873402-7 2006 beta(2)AR activation also increased NOS activity and phosphorylation of NOS-3 on serine-1177, and these increases were attenuated by inhibition of protein kinase A (PKA), phosphatidylinositol 3-kinase (PI3K) or Akt, and abolished by coinhibition of PKA and Akt. Serine 81-87 AKT serine/threonine kinase 1 Homo sapiens 257-260 16873402-8 2006 These findings suggest that beta(2)AR-mediated NOS-3 activation in HUVEC is mediated through phosphorylation of NOS-3 on serine-1177 through both the PKA and the PI3K/Akt systems, and is sustained by an increase in l-arginine uptake resulting from NO-mediated membrane hyperpolarization. Serine 121-127 AKT serine/threonine kinase 1 Homo sapiens 167-170 16987028-4 2006 In contrast, hydrogen peroxide-induced phosphorylation of Akt on serine 473 (S473) was downregulated by both PI3K and CaM-K inhibition, indicating that hydrogen peroxideinduced phosphorylation of Akt on S473 was largely dependent on both PI3K and a CaM-K activity. Serine 65-71 AKT serine/threonine kinase 1 Homo sapiens 58-61 16987028-4 2006 In contrast, hydrogen peroxide-induced phosphorylation of Akt on serine 473 (S473) was downregulated by both PI3K and CaM-K inhibition, indicating that hydrogen peroxideinduced phosphorylation of Akt on S473 was largely dependent on both PI3K and a CaM-K activity. Serine 65-71 AKT serine/threonine kinase 1 Homo sapiens 196-199 16760675-7 2006 Furthermore, SLD dose- and time-dependently inhibited the phosphorylation of Raf-1 on Ser 259, which is an established event by which Akt inhibits ERK1/2 activation. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 134-137 16737962-2 2006 In particular, phosphorylation of serine 1179 (bovine)/1177 (human) by Akt has been shown to be the central mechanism of eNOS regulation. Serine 34-40 AKT serine/threonine kinase 1 Homo sapiens 71-74 16785241-4 2006 Hyperactivation of Akt(Ser(P)473) was observed in PAI-1-/- EC, and this was probably due to enhanced inactivation of tumor suppressor PTEN, thus rendering the cells resistant to apoptotic signals. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 19-22 16234845-1 2006 Activation of protein kinase B (PKB, also known as Akt) by phosphorylation at serine-473 and threonine-308 promotes cell survival in multiple in vitro and in vivo models where neuronal death is seen, including traumatic brain injury (TBI); however, whether PKB is activated in humans after TBI was heretofore unknown. Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 51-54 16637063-7 2006 The activated Akt kinase was shown to directly phosphorylate ERalpha at its serine residues and cause ligand independent activation. Serine 76-82 AKT serine/threonine kinase 1 Homo sapiens 14-17 16670089-5 2006 C5b-9 activates Akt as shown by in vitro kinase assay and phosphorylation of Ser-473. Serine 77-80 AKT serine/threonine kinase 1 Homo sapiens 16-19 16679323-2 2006 We have recently discovered that Bax phosphorylation at serine 184 induced by nicotine through activation of protein kinase AKT abolishes its proapoptotic function in human lung cancer cells. Serine 56-62 AKT serine/threonine kinase 1 Homo sapiens 124-127 16731842-5 2006 Akt Ser(473) phosphorylation was increased (1.8-fold; P=0.011) after endurance but was unchanged after resistance exercise. Serine 4-7 AKT serine/threonine kinase 1 Homo sapiens 0-3 16780593-10 2006 P27 phosphorylated by Akt1 was detected by a phospho-S10 specific antibody, confirming this serine was targeted. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 22-26 16780593-14 2006 Phospho-serine was still present, however, confirming Akt1 sequentially targets multiple serines in this region. Serine 8-14 AKT serine/threonine kinase 1 Homo sapiens 54-58 16780593-14 2006 Phospho-serine was still present, however, confirming Akt1 sequentially targets multiple serines in this region. Serine 89-96 AKT serine/threonine kinase 1 Homo sapiens 54-58 16780593-22 2006 Using recombinant Akt1 recapitulating this specificity, we showed modification of p27S10 also leads to phosphorylation of an adjacent serine. Serine 134-140 AKT serine/threonine kinase 1 Homo sapiens 18-22 16721060-7 2006 Moreover, the cap-dependent repressor activity of YB-1 appears to be negatively regulated via Akt-mediated phosphorylation of the Ser-102 residue of YB-1. Serine 130-133 AKT serine/threonine kinase 1 Homo sapiens 94-97 16532036-6 2006 Furthermore, AKT phosphorylation of apoptosis signal-regulating kinase 1 (ASK1) on Ser-83 was reduced by DPI and siNox4RNAs. Serine 83-86 AKT serine/threonine kinase 1 Homo sapiens 13-16 16540596-6 2006 US stimulation increased the phosphorylation of focal adhesion kinase (FAK), extracellular signal-regulated kinases (ERK), p85 subunit of PI3K, and serine 473 of Akt. Serine 148-154 AKT serine/threonine kinase 1 Homo sapiens 162-165 16531397-2 2006 Upon growth factor receptor stimulation, cytosolic Akt is recruited to the plasma membrane by phospholipid binding and activated through phosphorylation at Thr(308) and Ser(473). Serine 169-172 AKT serine/threonine kinase 1 Homo sapiens 51-54 16620785-3 2006 In both differentiated hippocampal neurons and neuroblastoma SH-SY5Y cells we found that these two agents not only activated AMPK, but conversely greatly reduced the activating Ser/Thr phosphorylation of Akt. Serine 177-180 AKT serine/threonine kinase 1 Homo sapiens 204-207 16620785-4 2006 This blockade of Akt activity consequently lowered the inhibitory serine-phosphorylation of its substrates, glycogen synthase kinase-3alpha/beta (GSK3alpha/beta). Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 17-20 16551632-4 2006 Mass spectrometry analysis allowed the identification of VCP, and we have shown a serine/threonine phosphorylation on an Akt consensus site upon activation by growth factors. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 121-124 16551632-5 2006 Site-directed mutagenesis identified Ser-351, Ser-745, and Ser-747 as Akt phosphorylation sites on VCP. Serine 37-40 AKT serine/threonine kinase 1 Homo sapiens 70-73 16549426-3 2006 We here propose that the kinase activity of Akt is regulated via autophosphorylation in trans at two putative sites (threonine 72 and serine 246) that lie in the characteristic Akt substrate motif (RXRXX(S/T)). Serine 134-140 AKT serine/threonine kinase 1 Homo sapiens 44-47 16682621-8 2006 However, phosphorylation of AR by AKT at Ser-213 and Ser-791 is not critical for this synergy. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 34-37 16549426-3 2006 We here propose that the kinase activity of Akt is regulated via autophosphorylation in trans at two putative sites (threonine 72 and serine 246) that lie in the characteristic Akt substrate motif (RXRXX(S/T)). Serine 134-140 AKT serine/threonine kinase 1 Homo sapiens 177-180 16543232-3 2006 Exposure of cells to alpha2M* (50 pM) induced phosphatidylinositol 3-kinase-dependent activation of Akt by phosphorylation at Thr-308 and Ser-473 with a concomitant 60-80% increase in Akt-associated kinase activity. Serine 138-141 AKT serine/threonine kinase 1 Homo sapiens 100-103 16549426-6 2006 Autophosphorylation of Akt on Thr-72 and Ser-246 appeared to require prior phosphorylation of Akt on Thr-308 and Ser-473. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 23-26 16549426-6 2006 Autophosphorylation of Akt on Thr-72 and Ser-246 appeared to require prior phosphorylation of Akt on Thr-308 and Ser-473. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 94-97 16549426-6 2006 Autophosphorylation of Akt on Thr-72 and Ser-246 appeared to require prior phosphorylation of Akt on Thr-308 and Ser-473. Serine 113-116 AKT serine/threonine kinase 1 Homo sapiens 23-26 16549426-6 2006 Autophosphorylation of Akt on Thr-72 and Ser-246 appeared to require prior phosphorylation of Akt on Thr-308 and Ser-473. Serine 113-116 AKT serine/threonine kinase 1 Homo sapiens 94-97 16287065-6 2006 The activation state of the PI3K-AKT pathway was assessed immunohistochemically using an antibody with specificity towards serine 473-phosphorylated AKT. Serine 123-129 AKT serine/threonine kinase 1 Homo sapiens 33-36 16338973-1 2006 In endothelial cells, two ways of endothelial nitric oxide (NO) synthase (eNOS) activation are known: 1) translocation and 2) Akt-dependent phosphorylation of the enzyme at Ser(1177) (Ser(1177) eNOS). Serine 173-176 AKT serine/threonine kinase 1 Homo sapiens 126-129 16338973-1 2006 In endothelial cells, two ways of endothelial nitric oxide (NO) synthase (eNOS) activation are known: 1) translocation and 2) Akt-dependent phosphorylation of the enzyme at Ser(1177) (Ser(1177) eNOS). Serine 184-187 AKT serine/threonine kinase 1 Homo sapiens 126-129 16287065-6 2006 The activation state of the PI3K-AKT pathway was assessed immunohistochemically using an antibody with specificity towards serine 473-phosphorylated AKT. Serine 123-129 AKT serine/threonine kinase 1 Homo sapiens 149-152 16497409-10 2006 Surprisingly, the phosphorylation of Akt at serine 473 was increased in A549 cells treated with 7.5 microM OSU03013. Serine 44-50 AKT serine/threonine kinase 1 Homo sapiens 37-40 16545678-2 2006 Exposure of primary human lung microvascular endothelial cells (HLMVEC) to hyperoxia caused transient Akt activation after 60 min, as determined by Western blot analysis of phosphorylated Ser 473 of Akt. Serine 188-191 AKT serine/threonine kinase 1 Homo sapiens 102-105 16545678-2 2006 Exposure of primary human lung microvascular endothelial cells (HLMVEC) to hyperoxia caused transient Akt activation after 60 min, as determined by Western blot analysis of phosphorylated Ser 473 of Akt. Serine 188-191 AKT serine/threonine kinase 1 Homo sapiens 199-202 16537601-5 2006 (i) Infection of target cells with HSV-1 induces transient phosphorylation of serine 473 of PKB/Akt early in infection, with a mechanism that is dependent on PI3K. Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 92-95 16537601-5 2006 (i) Infection of target cells with HSV-1 induces transient phosphorylation of serine 473 of PKB/Akt early in infection, with a mechanism that is dependent on PI3K. Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 96-99 16585200-5 2006 Western blot analysis indicated that KP372-1 decreased the phosphorylation of AKT on both Ser(473) and Thr(308); abrogated the phosphorylation of p70S6 kinase, BAD, and Foxo3a via PI3K/AKT signaling; and down-regulated expression of PIM-1 through direct inhibition of FLT3. Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 78-81 16205782-10 2006 In line with these results, a Western blot study showed that these drugs increased phospho-Ser-473 Akt level, had no effect on total Akt, and decreased glycogen synthase kinase-3beta level. Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 99-102 16247451-6 2006 siRNAs targeting casein kinase I gamma 3 (CSNK1G3) or the inositol polyphosphate multikinase (IPMK) significantly enhanced A-443654-mediated cell killing, and caused decreases in Akt Ser-473 and ribosomal protein S6 phosphorylation. Serine 183-186 AKT serine/threonine kinase 1 Homo sapiens 179-182 16619521-7 2006 RESULTS: Treatment with Qu (25 microM) for 0.5, 1 and 3 h completely suppressed constitutively activated Akt/PKB phosphorylation at Ser-473 in HCC1937 cells. Serine 132-135 AKT serine/threonine kinase 1 Homo sapiens 105-108 16619521-8 2006 Pre-exposing T47D cells to Qu (25 microM) or LY (10 microM) abrogated EGF-induced Akt/PKB phosphorylation at Ser-473. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 86-89 16619521-7 2006 RESULTS: Treatment with Qu (25 microM) for 0.5, 1 and 3 h completely suppressed constitutively activated Akt/PKB phosphorylation at Ser-473 in HCC1937 cells. Serine 132-135 AKT serine/threonine kinase 1 Homo sapiens 109-112 16619521-8 2006 Pre-exposing T47D cells to Qu (25 microM) or LY (10 microM) abrogated EGF-induced Akt/PKB phosphorylation at Ser-473. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 82-85 16049961-6 2006 We evaluated Akt activation by immunohistochemical assessment of the expression of phosphorylated Akt (pAkt) at Ser-473. Serine 112-115 AKT serine/threonine kinase 1 Homo sapiens 13-16 16169930-4 2006 Treatment of PC-3/DU145 cells with apoptosis inducing concentration of DATS (40 microM) resulted in a rapid decrease in Ser(473) and Thr(308) phosphorylation of Akt leading to inhibition of its kinase activity. Serine 120-123 AKT serine/threonine kinase 1 Homo sapiens 161-164 16243840-9 2006 In vitro 32P-phosphorylation studies revealed that Akt phosphorylates CSP on serine 10. Serine 77-83 AKT serine/threonine kinase 1 Homo sapiens 51-54 16243840-11 2006 Taken together, these findings reveal a novel role for Akt phosphorylation in regulating the late stages of exocytosis and suggest that this is achieved via the phosphorylation of CSP on serine 10. Serine 187-193 AKT serine/threonine kinase 1 Homo sapiens 55-58 16421604-5 2006 In human PrCa, PTEN loss is correlated with substantial increases in Akt(Ser473) and integrin-linked kinase expression, both of which promote Ser(9) phospho-inhibition of GSK3beta and inactivation of apoptotic factors. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 69-72 16224792-6 2006 Subtle differences were also observed between insulin and zinc in the serine-473 phosphorylation of Akt. Serine 70-76 AKT serine/threonine kinase 1 Homo sapiens 100-103 16224792-7 2006 Zinc increased serine-473 phosphorylation of Akt, but to a lesser extent than insulin. Serine 15-21 AKT serine/threonine kinase 1 Homo sapiens 45-48 16243840-4 2006 Secretory stimuli in both intact and permeabilized cells induced Akt phosphorylation on serine 473, and the time course of Ca2+-induced Akt phosphorylation was similar to that of exocytosis in permeabilized cells. Serine 88-94 AKT serine/threonine kinase 1 Homo sapiens 65-68 16049961-6 2006 We evaluated Akt activation by immunohistochemical assessment of the expression of phosphorylated Akt (pAkt) at Ser-473. Serine 112-115 AKT serine/threonine kinase 1 Homo sapiens 98-101 16210317-2 2005 To examine the phosphorylation of AR at serine 213, a putative substrate for Akt, a phosphorylation site-specific antibody was generated. Serine 40-46 AKT serine/threonine kinase 1 Homo sapiens 77-80 16166268-7 2006 Substitution of Pro326 in the C terminus of murine CX3CR1 with Ser (residue found in the analogous position of human CX3CR1) produced a mutant receptor that mimicked the human receptor in its ability to stimulate the phosphorylation of both Akt and extracellular signal-regulated kinase in a time-, PI3K-, and pertussis toxin-sensitive G-protein-dependent manner. Serine 63-66 AKT serine/threonine kinase 1 Homo sapiens 241-244 16357133-5 2005 Our results show that Akt specifically phosphorylates Ser(67) and Ser(457) residues of Pdcd4 in vitro and in vivo. Serine 54-57 AKT serine/threonine kinase 1 Homo sapiens 22-25 16357133-5 2005 Our results show that Akt specifically phosphorylates Ser(67) and Ser(457) residues of Pdcd4 in vitro and in vivo. Serine 66-69 AKT serine/threonine kinase 1 Homo sapiens 22-25 16210322-8 2005 Incubation of hepatocytes with arachidonic acid blocks the activation of PI 3-kinase by insulin as observed by a decrease in Ser(473) phosphorylation of Akt, the downstream effector of PI 3-kinase. Serine 125-128 AKT serine/threonine kinase 1 Homo sapiens 153-156 17168719-6 2006 Moreover, it has been recently reported that nicotine in lung adenocarcinoma A549 cells, potently induces Bad phosphorylation at serine (S)112, S136 and S155 in a mechanism involving activation of MAPKs, ERK1/2, PI3K/AKT and PKA through the linking to alpha7-receptors [9]. Serine 129-135 AKT serine/threonine kinase 1 Homo sapiens 217-220 16221682-1 2005 The insulin-signaling pathway leading to the activation of Akt/protein kinase B has been well characterized except for a single step, the phosphorylation of Akt at Ser-473. Serine 164-167 AKT serine/threonine kinase 1 Homo sapiens 59-62 16221682-1 2005 The insulin-signaling pathway leading to the activation of Akt/protein kinase B has been well characterized except for a single step, the phosphorylation of Akt at Ser-473. Serine 164-167 AKT serine/threonine kinase 1 Homo sapiens 157-160 16221682-8 2005 Although purified DNA-PK could phosphorylate a peptide derived from Akt that contains amino acid Ser-473, it could not phosphorylate full-length Akt2. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 68-71 16210317-9 2005 In fetal tissue the AR-Ser(P)-213 antigen was present in epithelial cells of the urogenital sinus when endogenous androgen levels were high and activated Akt was prevalent, but absent at a later stage of development when endogenous androgen levels were low and Akt activation was minimal. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 154-157 16210317-9 2005 In fetal tissue the AR-Ser(P)-213 antigen was present in epithelial cells of the urogenital sinus when endogenous androgen levels were high and activated Akt was prevalent, but absent at a later stage of development when endogenous androgen levels were low and Akt activation was minimal. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 261-264 16245318-3 2005 METHODS: PKB/Akt activation was assessed by immunohistochemistry using a phosphorylation state-specific antibody (Ser 473) in tongue cancer and preneoplastic specimens of oral cavity. Serine 114-117 AKT serine/threonine kinase 1 Homo sapiens 9-12 16245318-3 2005 METHODS: PKB/Akt activation was assessed by immunohistochemistry using a phosphorylation state-specific antibody (Ser 473) in tongue cancer and preneoplastic specimens of oral cavity. Serine 114-117 AKT serine/threonine kinase 1 Homo sapiens 13-16 16044149-5 2005 The use of specific antibodies shows that some proportion of PKB/Akt that is phosphorylated both on threonine 308 and serine 473 is maintained in the presence of E7 in a PI3 kinase-independent manner, and is activated for phosphorylation of BAD, a known downstream target of PKB/Akt. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 65-68 16015604-3 2005 EGFR activates NF-kappaB through the PI3K/Akt pathway that leads to the phosphorylation of IkappaBalpha on serines 32 and 36, thereby promoting the nuclear translocation of the p65 subunit. Serine 107-114 AKT serine/threonine kinase 1 Homo sapiens 42-45 16044149-5 2005 The use of specific antibodies shows that some proportion of PKB/Akt that is phosphorylated both on threonine 308 and serine 473 is maintained in the presence of E7 in a PI3 kinase-independent manner, and is activated for phosphorylation of BAD, a known downstream target of PKB/Akt. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 61-64 16044149-5 2005 The use of specific antibodies shows that some proportion of PKB/Akt that is phosphorylated both on threonine 308 and serine 473 is maintained in the presence of E7 in a PI3 kinase-independent manner, and is activated for phosphorylation of BAD, a known downstream target of PKB/Akt. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 279-282 16172130-3 2005 Incubation of macrophages with forskolin triggered the activation of Akt, predominantly by phosphorylation of Ser-473, as measured by Western blotting and assay of its kinase activity. Serine 110-113 AKT serine/threonine kinase 1 Homo sapiens 69-72 16169518-8 2005 Treatment of MCF-7 cells with E2 resulted in a marked increase in PI3K (p85) expression, which paralleled an increase in phospho-Akt (Ser-473) and PIP(3) level. Serine 134-137 AKT serine/threonine kinase 1 Homo sapiens 129-132 16007122-7 2005 Collectively, these results suggest that both Stat3 and AKT provide survival signals in U87 and D54 cells, and Ser/Thr phosphorylation of Stat3-TAD by the PI3K-AKT pathway negatively controls the DNA-binding function of Stat3. Serine 111-114 AKT serine/threonine kinase 1 Homo sapiens 160-163 16172130-7 2005 Silencing of CREB gene expression by RNA interference prior to forskolin treatment not only decreased CREB protein and its phosphorylation at Ser-133, but also phosphorylation of Akt at Ser-473, and Thr-308. Serine 186-189 AKT serine/threonine kinase 1 Homo sapiens 179-182 16177823-4 2005 We demonstrate that Akt phosphorylation of acinus on serine 422 and 573 results in its resistance to caspase cleavage in the nucleus and the inhibition of acinus-dependent chromatin condensation. Serine 53-59 AKT serine/threonine kinase 1 Homo sapiens 20-23 16182322-5 2005 In the present study, PDGF increased the level of Akt phosphorylation (Ser 473) in C6 glioma cells, a cell line that has been used to study regulated trafficking of endogenous EAAC1. Serine 71-74 AKT serine/threonine kinase 1 Homo sapiens 50-53 16009706-2 2005 In cellular assays at 5 mum, 17 compounds inhibited insulin-like growth factor 1 (IGF-I)-stimulated phosphorylation of Akt (Ser-473) by at least 50% but did not inhibit IGF-I-stimulated phosphorylation of Erk-1/2 (Thr-202/Tyr-204). Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 119-122 16224021-2 2005 Here, we show that Akt phosphorylates EZH2 at serine 21 and suppresses its methyltransferase activity by impeding EZH2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes. Serine 46-52 AKT serine/threonine kinase 1 Homo sapiens 19-22 16118242-6 2005 While the shear-stress-induced tyrosine phosphorylation of PECAM-1 as well as the serine phosphorylation of Akt and eNOS were abolished by the pre-treatment of cells with the tyrosine kinase inhibitor PP1 the phosphorylation of AMPK was unaffected. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 108-111 16009713-2 2005 Previous studies established that IFNalpha/beta activates NF-kappaB to promote cell survival through a phosphatidylinositol 3-kinase (PI3K)/Akt pathway, which involves serine phosphorylation and degradation of IkappaB alpha. Serine 168-174 AKT serine/threonine kinase 1 Homo sapiens 140-143 16009706-4 2005 Inhibition of Akt phosphorylation was associated with the inhibition of IGF-I stimulation of the mammalian target of rapamycin phosphorylation (Ser-2448 and Ser-2481), phosphorylation of p70 S6 kinase (Thr-389), and ribosomal protein S6 (Ser-235/236) in Rh1, Rh18, and Rh30 cell lines. Serine 144-147 AKT serine/threonine kinase 1 Homo sapiens 14-17 16009706-4 2005 Inhibition of Akt phosphorylation was associated with the inhibition of IGF-I stimulation of the mammalian target of rapamycin phosphorylation (Ser-2448 and Ser-2481), phosphorylation of p70 S6 kinase (Thr-389), and ribosomal protein S6 (Ser-235/236) in Rh1, Rh18, and Rh30 cell lines. Serine 157-160 AKT serine/threonine kinase 1 Homo sapiens 14-17 16009706-4 2005 Inhibition of Akt phosphorylation was associated with the inhibition of IGF-I stimulation of the mammalian target of rapamycin phosphorylation (Ser-2448 and Ser-2481), phosphorylation of p70 S6 kinase (Thr-389), and ribosomal protein S6 (Ser-235/236) in Rh1, Rh18, and Rh30 cell lines. Serine 157-160 AKT serine/threonine kinase 1 Homo sapiens 14-17 16024795-0 2005 Akt phosphorylation of p300 at Ser-1834 is essential for its histone acetyltransferase and transcriptional activity. Serine 31-34 AKT serine/threonine kinase 1 Homo sapiens 0-3 16139227-5 2005 Akt phosphorylates serine at position 1416 in Girdin, and phosphorylated Girdin accumulates at the leading edge of migrating cells. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 0-3 16034301-6 2005 Immunohistochemistry for phospho-Akt was performed using an anti-phospho-Akt (Ser 473) rabbit antibody. Serine 78-81 AKT serine/threonine kinase 1 Homo sapiens 33-36 16024795-4 2005 Nuclear Akt associates with p300 and phosphorylates its Ser-1834 both in vivo and in vitro. Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 8-11 15996564-5 2005 The involvement of PI3-K/Akt pathway in the imipramine action was confirmed by Western blot study, which showed that this drug increased phospho-Ser-473 Akt level, but had no effect on total Akt and glycogen synthase kinase (GSK-3beta) levels. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 25-28 15996564-5 2005 The involvement of PI3-K/Akt pathway in the imipramine action was confirmed by Western blot study, which showed that this drug increased phospho-Ser-473 Akt level, but had no effect on total Akt and glycogen synthase kinase (GSK-3beta) levels. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 153-156 15996564-5 2005 The involvement of PI3-K/Akt pathway in the imipramine action was confirmed by Western blot study, which showed that this drug increased phospho-Ser-473 Akt level, but had no effect on total Akt and glycogen synthase kinase (GSK-3beta) levels. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 153-156 15998322-6 2005 By mutagenesis analysis and subsequent immunoprecipitation (IP), we established that Akt phosphorylates JFC1 at serine 241. Serine 112-118 AKT serine/threonine kinase 1 Homo sapiens 85-88 15911620-7 2005 Dissociated Akt1 binds to SEK1 and ASK1 and inhibits their enzyme activity by phosphorylating SEK1 on the Ser-80 residue and ASK1 on the Ser-83 residue. Serine 106-109 AKT serine/threonine kinase 1 Homo sapiens 12-16 15911620-7 2005 Dissociated Akt1 binds to SEK1 and ASK1 and inhibits their enzyme activity by phosphorylating SEK1 on the Ser-80 residue and ASK1 on the Ser-83 residue. Serine 137-140 AKT serine/threonine kinase 1 Homo sapiens 12-16 15960984-3 2005 Recent work has identified novel pathways, such as the Wnt pathway and the serine-threonine kinase Akt, as central modulators that oversee cellular apoptosis and the formation of neurofibrillary tangles through their downstream substrates that include glycogen synthase kinase-3beta, Bad, and Bcl-xL. Serine 75-81 AKT serine/threonine kinase 1 Homo sapiens 99-102 15899889-3 2005 Previous studies have shown that mTOR is a direct substrate for the AKT kinase and identified Ser-2448 as the AKT target site in mTOR. Serine 94-97 AKT serine/threonine kinase 1 Homo sapiens 68-71 15899889-3 2005 Previous studies have shown that mTOR is a direct substrate for the AKT kinase and identified Ser-2448 as the AKT target site in mTOR. Serine 94-97 AKT serine/threonine kinase 1 Homo sapiens 110-113 15913552-5 2005 A recent study presents evidence that Akt phosphorylates Synip on serine 99, at least in vitro, and proposes that this phosphorylation enables GLUT4 translocation by causing the dissociation of Synip from syntaxin4. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 38-41 15799972-6 2005 Treatment with wortmannin or calphostin C or the expression of dominant negative Akt inhibited phosphorylation of GSK-3beta at Ser-9 following the KCl-induced depolarization of SH-SY5Y cells. Serine 127-130 AKT serine/threonine kinase 1 Homo sapiens 81-84 16006742-2 2005 FKHR is phosphorylated on three sites (Thr-24, Ser-256, and Ser-319) in a phosphatidylinositol 3-kinase (PI3K)/Akt-dependent manner, thereby inhibiting death signals. Serine 47-50 AKT serine/threonine kinase 1 Homo sapiens 111-114 16006742-2 2005 FKHR is phosphorylated on three sites (Thr-24, Ser-256, and Ser-319) in a phosphatidylinositol 3-kinase (PI3K)/Akt-dependent manner, thereby inhibiting death signals. Serine 60-63 AKT serine/threonine kinase 1 Homo sapiens 111-114 15964826-7 2005 Serine-642 was identified as an Akt-dependent phosphorylation site. Serine 0-6 AKT serine/threonine kinase 1 Homo sapiens 32-35 15809304-3 2005 This neuroprotective effect involves the phosphorylation of huntingtin at Ser(421) by the prosurvival kinase Akt (Humbert, S., Bryson, E. A., Cordelieres, F. P., Connors, N. C., Datta, S. R., Finkbeiner, S., Greenberg, M. E., and Saudou, F. (2002) Dev. Serine 74-77 AKT serine/threonine kinase 1 Homo sapiens 109-112 15809304-7 2005 Akt phosphorylated arfaptin 2 at Ser(260). Serine 33-36 AKT serine/threonine kinase 1 Homo sapiens 0-3 15802620-2 2005 Recently, serine phosphorylation and degradation of insulin receptor substrate-1 (IRS-1) were shown to inhibit Akt activation and reduce glucose uptake. Serine 10-16 AKT serine/threonine kinase 1 Homo sapiens 111-114 15805282-6 2005 Intracellular accumulation correlated temporally with inhibition of p-Ser(473) Akt and GSK-3alpha/beta phosphorylation, suggesting that Ser(473) is an Akt autophosphorylation site. Serine 136-139 AKT serine/threonine kinase 1 Homo sapiens 151-154 15534860-3 2005 We found that HGF/Cpd 5-induced ERK phosphorylation and cell growth inhibition were mediated by Akt (protein kinase B) pathway, since combination HGF/Cpd 5 treatment of Hep3B cells inhibited Akt phosphorylation at Ser-473 and its kinase activity, which led to the suppression of Raf-1 phosphorylation at Ser-259. Serine 214-217 AKT serine/threonine kinase 1 Homo sapiens 96-99 15534860-3 2005 We found that HGF/Cpd 5-induced ERK phosphorylation and cell growth inhibition were mediated by Akt (protein kinase B) pathway, since combination HGF/Cpd 5 treatment of Hep3B cells inhibited Akt phosphorylation at Ser-473 and its kinase activity, which led to the suppression of Raf-1 phosphorylation at Ser-259. Serine 304-307 AKT serine/threonine kinase 1 Homo sapiens 96-99 15657900-8 2005 Moreover, the combined treatment of C75 and Taxol inactivated the anti-apoptotic AKT (protein kinase B) kinase more than either agent alone, as evidenced by a synergistic down-regulation of AKT phosphorylation at its activating site Ser(473) without affecting AKT protein levels. Serine 233-236 AKT serine/threonine kinase 1 Homo sapiens 81-84 15657900-8 2005 Moreover, the combined treatment of C75 and Taxol inactivated the anti-apoptotic AKT (protein kinase B) kinase more than either agent alone, as evidenced by a synergistic down-regulation of AKT phosphorylation at its activating site Ser(473) without affecting AKT protein levels. Serine 233-236 AKT serine/threonine kinase 1 Homo sapiens 190-193 15657900-8 2005 Moreover, the combined treatment of C75 and Taxol inactivated the anti-apoptotic AKT (protein kinase B) kinase more than either agent alone, as evidenced by a synergistic down-regulation of AKT phosphorylation at its activating site Ser(473) without affecting AKT protein levels. Serine 233-236 AKT serine/threonine kinase 1 Homo sapiens 190-193 15738003-8 2005 Phosphorylation of Akt/PKB at Ser-473 and Thr-308 was inhibited by latrunculin B treatment, indicating that the defect in signaling lies prior to Akt/PKB activation. Serine 30-33 AKT serine/threonine kinase 1 Homo sapiens 19-26 15738003-8 2005 Phosphorylation of Akt/PKB at Ser-473 and Thr-308 was inhibited by latrunculin B treatment, indicating that the defect in signaling lies prior to Akt/PKB activation. Serine 30-33 AKT serine/threonine kinase 1 Homo sapiens 19-22 15738003-8 2005 Phosphorylation of Akt/PKB at Ser-473 and Thr-308 was inhibited by latrunculin B treatment, indicating that the defect in signaling lies prior to Akt/PKB activation. Serine 30-33 AKT serine/threonine kinase 1 Homo sapiens 23-26 15862750-3 2005 Immunohistochemical expression of serine 473 specific phosphorylated Akt (pAkt) isoforms (Akt-1,2,3) was evaluated in 97 patients, together with subcellular expression of p53/Hdm2. Serine 34-40 AKT serine/threonine kinase 1 Homo sapiens 69-72 15862750-3 2005 Immunohistochemical expression of serine 473 specific phosphorylated Akt (pAkt) isoforms (Akt-1,2,3) was evaluated in 97 patients, together with subcellular expression of p53/Hdm2. Serine 34-40 AKT serine/threonine kinase 1 Homo sapiens 90-99 15805282-6 2005 Intracellular accumulation correlated temporally with inhibition of p-Ser(473) Akt and GSK-3alpha/beta phosphorylation, suggesting that Ser(473) is an Akt autophosphorylation site. Serine 70-73 AKT serine/threonine kinase 1 Homo sapiens 79-82 15805282-6 2005 Intracellular accumulation correlated temporally with inhibition of p-Ser(473) Akt and GSK-3alpha/beta phosphorylation, suggesting that Ser(473) is an Akt autophosphorylation site. Serine 136-139 AKT serine/threonine kinase 1 Homo sapiens 79-82 15919790-10 2005 Impaired AS160 phosphorylation was related to aberrant Akt signaling; insulin action on Akt Ser(473) phosphorylation was not significantly reduced in type 2 diabetic compared with control subjects, whereas Thr(308) phosphorylation was impaired 51% (P < 0.05). Serine 92-95 AKT serine/threonine kinase 1 Homo sapiens 88-91 15782140-2 2005 PTEN mutation leads to constitutive activation of protein kinase B (PKB)/Akt with phosphorylation at the PKB/Akt sites Thr-308 and Ser-473. Serine 131-134 AKT serine/threonine kinase 1 Homo sapiens 68-71 15782140-2 2005 PTEN mutation leads to constitutive activation of protein kinase B (PKB)/Akt with phosphorylation at the PKB/Akt sites Thr-308 and Ser-473. Serine 131-134 AKT serine/threonine kinase 1 Homo sapiens 73-76 15782140-2 2005 PTEN mutation leads to constitutive activation of protein kinase B (PKB)/Akt with phosphorylation at the PKB/Akt sites Thr-308 and Ser-473. Serine 131-134 AKT serine/threonine kinase 1 Homo sapiens 109-112 15782140-6 2005 Transfection of ILK antisense (ILKAS) or exposure to a small-molecule ILK inhibitor suppresses the constitutive phosphorylation of PKB/Akt on Ser-473 in PTEN-mutant glioblastoma cell lines. Serine 142-145 AKT serine/threonine kinase 1 Homo sapiens 131-134 15782140-6 2005 Transfection of ILK antisense (ILKAS) or exposure to a small-molecule ILK inhibitor suppresses the constitutive phosphorylation of PKB/Akt on Ser-473 in PTEN-mutant glioblastoma cell lines. Serine 142-145 AKT serine/threonine kinase 1 Homo sapiens 135-138 15781227-5 2005 HGF elicited PI3K-Akt activation and inhibited GSK3, a downstream transducer of PI3K-Akt, by inhibitory phosphorylation at Ser-9. Serine 123-126 AKT serine/threonine kinase 1 Homo sapiens 85-88 15735038-4 2005 There was also suppression in phosphorylated PKB Thr(308), forkhead in rhabdomyosarcoma, S6K1, S6, 4E-BP1, and signal transducers and activators of transcription 3 Tyr(705) and Ser(727) protein levels with ILK inhibition by QLT0254. Serine 177-180 AKT serine/threonine kinase 1 Homo sapiens 45-48 15706421-4 2005 In A549 cells, FK228 suppressed the phosphorylation of Akt at Ser-473 and glycogen synthase kinase-3 without affecting these protein levels, indicating inhibition of the Akt-mediated signaling pathway. Serine 62-65 AKT serine/threonine kinase 1 Homo sapiens 55-58 15733940-5 2005 We found that treatment of EC with a low dose of pitavastatin induced eNOS phosphorylation at Ser-1177, activated Akt phosphorylation at Ser-473 in a time-and dose-dependent manner, and increased NO production. Serine 137-140 AKT serine/threonine kinase 1 Homo sapiens 114-117 15546863-5 2005 This effect is mediated through the phosphatidylinositol 3-kinase domain of ATM that specifically affects Akt serine 473 phosphorylation. Serine 110-116 AKT serine/threonine kinase 1 Homo sapiens 106-109 15710380-4 2005 Changing them from Ser, Glu and Ser to Phe, Ala and Pro, respectively, transformed the nonapeptide into an excellent substrate for PKBalpha and RSK1. Serine 19-22 AKT serine/threonine kinase 1 Homo sapiens 131-139 15710380-4 2005 Changing them from Ser, Glu and Ser to Phe, Ala and Pro, respectively, transformed the nonapeptide into an excellent substrate for PKBalpha and RSK1. Serine 32-35 AKT serine/threonine kinase 1 Homo sapiens 131-139 15546863-8 2005 Further supporting these results, we also observed that biological responses tightly regulated by Akt, such as transcription factor of the forkhead family activity after insulin treatment or gamma-radiation response, were altered in cell lines derived from AT patients and knockout mice for ATM in which phosphorylation in serine 473 was almost abolished. Serine 323-329 AKT serine/threonine kinase 1 Homo sapiens 98-101 15537647-5 2005 DEX decreased the serum-responsive phosphorylation of protein kinase B/Akt-Ser(473) within minutes, and this inhibition was also observed after 12 h. When the phosphatidylinositol 3-kinase (PI3K)/Akt pathway was inhibited by wortmannin, DEX no longer inhibited beta-catenin levels. Serine 75-78 AKT serine/threonine kinase 1 Homo sapiens 71-74 15545271-4 2005 It was demonstrated that mechanical stretch rapidly induced the phosphorylation of the proto-oncogene protein kinase B (PKB)/Akt at both phosphorylation sites (serine 473/threonine 308) in different epithelial cells (HaCaT, A-431, and human embryonic kidney-293). Serine 160-166 AKT serine/threonine kinase 1 Homo sapiens 120-123 15545271-4 2005 It was demonstrated that mechanical stretch rapidly induced the phosphorylation of the proto-oncogene protein kinase B (PKB)/Akt at both phosphorylation sites (serine 473/threonine 308) in different epithelial cells (HaCaT, A-431, and human embryonic kidney-293). Serine 160-166 AKT serine/threonine kinase 1 Homo sapiens 125-128 15710331-2 2005 The checkpoint defect was traced to the ability of AKT to phosphorylate CHK1 at serine 280, since a nonphosphorylated mutant of CHK1 (S280A) complemented the checkpoint defect and restored CDC25A degradation. Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 51-54 15882775-6 2005 Current work has identified exciting pathways, such as the Wnt pathway and the serine-threonine kinase Akt, as central modulators that oversee cellular apoptosis and their downstream substrates that include Forkhead transcription factors, glycogen synthase kinase-3beta, mitochondrial dysfunction, Bad, and Bcl-x(L). Serine 79-85 AKT serine/threonine kinase 1 Homo sapiens 103-106 15671247-5 2005 Overexpression of AND-34 in MCF-7 cells increased PI3K activity and augmented Akt Ser(473) phosphorylation and kinase activity. Serine 82-85 AKT serine/threonine kinase 1 Homo sapiens 78-81 15733744-3 2005 The activation of PKB in response to insulin propagates insulin signaling and promotes the phosphorylation of IRS1 on serine residue in turn generating a positive-feedback loop for insulin action. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 18-21 15235105-6 2004 Expression of an activated AKT (Myr-AKT2) increased basal phospho-p70S6K (Thr(389) and Thr(421)/Ser(424)) content, but failed to stimulate cell migration. Serine 96-99 AKT serine/threonine kinase 1 Homo sapiens 27-30 15364919-4 2004 Here we show that PKC phosphorylates IRS1 at serine 1101 blocking IRS1 tyrosine phosphorylation and downstream activation of the Akt pathway. Serine 45-51 AKT serine/threonine kinase 1 Homo sapiens 129-132 15372623-4 2004 Basal as well as insulin-stimulated levels of Ser(473) and Thr(308) phosphorylation in PKB-alpha transfected HepG2 cells were much higher than HepG2 cells. Serine 46-49 AKT serine/threonine kinase 1 Homo sapiens 87-96 15327518-0 2004 Detection of serine 473 phosphorylated Akt in acute myeloid leukaemia blasts by flow cytometry. Serine 13-19 AKT serine/threonine kinase 1 Homo sapiens 39-42 15327518-2 2004 Akt kinase is activated by phosphorylation on Thr 308 and Ser 473. Serine 58-61 AKT serine/threonine kinase 1 Homo sapiens 0-3 15327518-5 2004 This work aimed to establish whether it was possible to detect Akt phosphorylation on Ser 473 of AML blasts by means of flow cytometry. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 63-66 15327518-8 2004 Double immunostaining enabled the simultaneous flow cytometric detection of an AML-associated antigen (CD33) and Ser 473 phosphorylated Akt in leukaemic blast populations. Serine 113-116 AKT serine/threonine kinase 1 Homo sapiens 136-139 15327518-9 2004 Our results indicate that flow cytometry enabled the rapid and quantitative assessment of Ser 473 phosphorylated Akt of AML blasts that, when used in combination with cell surface staining, can provide more accurate phenotyping of AML blasts. Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 113-116 15574783-5 2004 Naltrindole treatment reduced constitutive phosphorylation of Akt/PKB on serine 473 and threonine 308 in cells. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 62-65 15574783-5 2004 Naltrindole treatment reduced constitutive phosphorylation of Akt/PKB on serine 473 and threonine 308 in cells. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 66-69 15574783-6 2004 We found that the levels of constitutive phosphorylation of Akt/PKB on serine 473 correlate with the sensitivity of the three cell lines to naltrindole treatment. Serine 71-77 AKT serine/threonine kinase 1 Homo sapiens 60-63 15574783-6 2004 We found that the levels of constitutive phosphorylation of Akt/PKB on serine 473 correlate with the sensitivity of the three cell lines to naltrindole treatment. Serine 71-77 AKT serine/threonine kinase 1 Homo sapiens 64-67 15528359-5 2004 A deletion mutant (Bax(1-171)) without the C terminus mitochondrial targeting sequence or an Akt target site (Ser(184)) localizes to the cytoplasm and triggers low level apoptosis that is enhanced by DN-Akt or DN-MEK1. Serine 110-113 AKT serine/threonine kinase 1 Homo sapiens 93-96 15364915-0 2004 Protein kinase C betaII regulates Akt phosphorylation on Ser-473 in a cell type- and stimulus-specific fashion. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 34-37 15364915-3 2004 Membrane-bound Akt is then phosphorylated at two sites for its full activation; Thr-308 in the activation loop of the kinase domain is phosphorylated by 3-phosphoinositide-dependent kinase-1 (PDK1) and Ser-473 in the C-terminal hydrophobic motif by a putative kinase PDK2. Serine 202-205 AKT serine/threonine kinase 1 Homo sapiens 15-18 15364915-5 2004 Here we present evidence that conventional isoforms of protein kinase C (PKC), particularly PKCbetaII, can regulate Akt activity by directly phosphorylating Ser-473 in vitro and in IgE/antigen-stimulated mast cells. Serine 157-160 AKT serine/threonine kinase 1 Homo sapiens 116-119 15527798-3 2004 In the present study, we identify Ser-166, a site previously reported as an AKT target, and Ser-188, a novel site which is the major site of phosphorylation of MDM2 by AKT in vitro. Serine 92-95 AKT serine/threonine kinase 1 Homo sapiens 168-171 15527798-4 2004 Analysis of MDM2 in cultured cells confirms that Ser-166 and Ser-188 are phosphorylated by AKT in a physiological context. Serine 49-52 AKT serine/threonine kinase 1 Homo sapiens 91-94 15527798-4 2004 Analysis of MDM2 in cultured cells confirms that Ser-166 and Ser-188 are phosphorylated by AKT in a physiological context. Serine 61-64 AKT serine/threonine kinase 1 Homo sapiens 91-94 15319269-3 2004 METHODS AND RESULTS: In human endothelial cells, the Rho-kinase inhibitor, hydroxyfasudil (HF) (1 to 100 micromol/L), increased Akt serine-473 phosphorylation within 15 minutes, leading to a 2.2-fold and 4.0-fold increase in Akt kinase activity and nitric oxide (NO) release, respectively. Serine 132-138 AKT serine/threonine kinase 1 Homo sapiens 128-131 15309026-0 2004 beta1-Integrins induce phosphorylation of Akt on serine 473 independently of focal adhesion kinase and Src family kinases. Serine 49-55 AKT serine/threonine kinase 1 Homo sapiens 42-45 15235105-6 2004 Expression of an activated AKT (Myr-AKT2) increased basal phospho-p70S6K (Thr(389) and Thr(421)/Ser(424)) content, but failed to stimulate cell migration. Serine 96-99 AKT serine/threonine kinase 1 Homo sapiens 32-40 15082754-3 2004 Activated Akt phosphorylates human eNOS at serine 1177 and subsequently increases NOS activity. Serine 43-49 AKT serine/threonine kinase 1 Homo sapiens 10-13 15084475-5 2004 Inhibition of p38 MAPK activity with SB-203580 dose-dependently inhibits Akt phosphorylation on Ser(473), but not Thr(308). Serine 96-99 AKT serine/threonine kinase 1 Homo sapiens 73-76 15084475-8 2004 Finally, intracellular delivery of a MAPKAPK-2 peptide inhibitor blocks Akt phosphorylation on Ser(473). Serine 95-98 AKT serine/threonine kinase 1 Homo sapiens 72-75 15291810-6 2004 Coexpression of HA-Akt augmented dHAND phosphorylation at multiple serine and threonine residues mainly located in the bHLH domain and, as a result, decreased the transcriptional activity of dHAND. Serine 67-73 AKT serine/threonine kinase 1 Homo sapiens 19-22 15211372-6 2004 RESULTS: Adipocyte-conditioned medium strongly inhibited insulin-induced serine phosphorylation of Akt in myocytes with a rapid parallel activation of the nuclear factor kappaB pathway in these cells. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 99-102 15289327-5 2004 Cytoplasmic mislocalization of p27Kip1 was significantly associated with the constitutive serine(473) Akt/PKB phosphorylation in AML cells (P < 0.05). Serine 90-96 AKT serine/threonine kinase 1 Homo sapiens 102-109 15289328-1 2004 We previously showed, by immunohistochemistry with phospho-specific antibodies, increased phosphorylation (activation) of Akt (Ser(473)) [phosphorylated Akt (pAkt)] in high-Gleason grade prostate cancer (Malik SN, et al., Clin Cancer Res 2002;8:1168-71). Serine 127-130 AKT serine/threonine kinase 1 Homo sapiens 122-125 15289328-1 2004 We previously showed, by immunohistochemistry with phospho-specific antibodies, increased phosphorylation (activation) of Akt (Ser(473)) [phosphorylated Akt (pAkt)] in high-Gleason grade prostate cancer (Malik SN, et al., Clin Cancer Res 2002;8:1168-71). Serine 127-130 AKT serine/threonine kinase 1 Homo sapiens 153-156 15178423-7 2004 Increases in Akt activity were accompanied by increases in Akt Thr(308) and Ser(473) phosphorylation, decreased GSK3alpha activity ( approximately 30% at both intensities), and increased phosphorylation of GSK3alpha Ser(21). Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 13-16 15183529-0 2004 Positive correlation between overexpression of phospho-BAD with phosphorylated Akt at serine 473 but not threonine 308 in colorectal carcinoma. Serine 86-92 AKT serine/threonine kinase 1 Homo sapiens 79-82 15178423-7 2004 Increases in Akt activity were accompanied by increases in Akt Thr(308) and Ser(473) phosphorylation, decreased GSK3alpha activity ( approximately 30% at both intensities), and increased phosphorylation of GSK3alpha Ser(21). Serine 216-219 AKT serine/threonine kinase 1 Homo sapiens 13-16 15026413-4 2004 The main objective of the present study was to investigate the potential involvement of the serine threonine kinase Akt (also known as protein kinase B) signaling pathway in the mechanisms of beta-synuclein neuroprotection. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 116-119 15122336-5 2004 Also, while MAPK and p38 kinase inhibitors did not alter the invasive behavior of these cells, an increase in the phosphorylation of Akt at serine-473 was detected in N-cadherin transfectants, suggestive of N-cadherin-mediated Akt activation in bladder cell invasion. Serine 140-146 AKT serine/threonine kinase 1 Homo sapiens 133-136 15015934-6 2004 The suppressive effects of HC-gp39 were dependent on phosphoinositide 3-kinase activity, and treatment of cells with HC-gp39 resulted in AKT-mediated serine/threonine phosphorylation of apoptosis signal-regulating kinase 1. Serine 150-156 AKT serine/threonine kinase 1 Homo sapiens 137-140 15047712-9 2004 Furthermore, LGI1 expression promotes phosphorylation of AKT, which leads to phosphorylation of Raf1(Ser-259), an event shown previously to negatively regulate ERK1/2 signaling. Serine 101-104 AKT serine/threonine kinase 1 Homo sapiens 57-60 14990993-5 2004 Treatment of HMEC-E6 cells with 1.0 microM QTam resulted in a 50% decrease in mitochondrial transmembrane potential, sequential activation of caspase-9 and -3, and a 90% decrease in AKT Ser-473 phosphorylation. Serine 186-189 AKT serine/threonine kinase 1 Homo sapiens 182-185 14766748-6 2004 We suggest that Bax is regulated by phosphorylation of Ser(184) in an Akt-dependent manner and that phosphorylation inhibits Bax effects on the mitochondria by maintaining the protein in the cytoplasm, heterodimerized with antiapoptotic Bcl-2 family members. Serine 55-58 AKT serine/threonine kinase 1 Homo sapiens 70-73 14990993-7 2004 These data indicate that Tam and QTam induce apoptosis in HMEC-E6 cells through a plasma membrane-activated AKT-signaling pathway that results in (1) decreased AKT phosphorylation at Ser-473, (2) mitochondrial membrane depolarization, and (3) activated caspase-9 and -3. Serine 183-186 AKT serine/threonine kinase 1 Homo sapiens 108-111 14990993-7 2004 These data indicate that Tam and QTam induce apoptosis in HMEC-E6 cells through a plasma membrane-activated AKT-signaling pathway that results in (1) decreased AKT phosphorylation at Ser-473, (2) mitochondrial membrane depolarization, and (3) activated caspase-9 and -3. Serine 183-186 AKT serine/threonine kinase 1 Homo sapiens 160-163 15047168-7 2004 Phosphorylation of Akt at serine 473 and its downstream molecular Bad at serine 136 was induced by HAPO, but was blocked by two PI3K inhibitors, LY294002 and wortmannin. Serine 26-32 AKT serine/threonine kinase 1 Homo sapiens 19-22 15076714-1 2004 Enzyme activities of the serine/threonine kinase Akt were compared in mid-temporal and mid-frontal cortices from Alzheimer"s disease cases and matched controls. Serine 25-31 AKT serine/threonine kinase 1 Homo sapiens 49-52 15076714-5 2004 Strong Ser Akt immunoreactivity was seen in Alzheimer"s disease pyramidal neurons likely undergoing degeneration and in reactive astroglia. Serine 7-10 AKT serine/threonine kinase 1 Homo sapiens 11-14 15107605-1 2004 We have shown recently that DNA damage effector kinase Chk1 is phosphorylated in vitro by protein kinase B/Akt (PKB/Akt) on serine 280. Serine 124-130 AKT serine/threonine kinase 1 Homo sapiens 112-115 15107605-3 2004 In this study we show that Chk1 is phosphorylated by PKB in vivo, and that increased phosphorylation by PKB on serine 280 correlates with impairment of Chk1 activation by DNA damage. Serine 111-117 AKT serine/threonine kinase 1 Homo sapiens 53-56 15107605-3 2004 In this study we show that Chk1 is phosphorylated by PKB in vivo, and that increased phosphorylation by PKB on serine 280 correlates with impairment of Chk1 activation by DNA damage. Serine 111-117 AKT serine/threonine kinase 1 Homo sapiens 104-107 15107605-5 2004 The Chk1 protein phosphorylated by PKB on serine 280 does not enter into protein complexes after replication arrest. Serine 42-48 AKT serine/threonine kinase 1 Homo sapiens 35-38 15100319-3 2004 In these experiments, we found activation of Akt in neutrophils stimulated with the TLR2-specific ligands peptidoglycan and the lipopeptide tri-palmitoyl-S-glyceryl-Cys-Ser-(Lys)(4) that occurred earlier and was of greater magnitude than that present after exposure to the TLR4 agonist LPS. Serine 169-172 AKT serine/threonine kinase 1 Homo sapiens 45-48 15047168-7 2004 Phosphorylation of Akt at serine 473 and its downstream molecular Bad at serine 136 was induced by HAPO, but was blocked by two PI3K inhibitors, LY294002 and wortmannin. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 19-22 15048882-5 2004 H2O2-induced Akt ser-473 phosphorylation and upregulation of Ran protein were prevented by wortmannin but not by rapamycin, indicating that PI3K is upstream of Akt and mTOR is downstream from Akt. Serine 17-20 AKT serine/threonine kinase 1 Homo sapiens 13-16 15044083-3 2004 TGFbeta-mediated phosphorylation of Akt at Ser-473 was inhibited by dominant-negative ILK and PI3K inhibitors, LY294002 and wortmannin. Serine 43-46 AKT serine/threonine kinase 1 Homo sapiens 36-39 15048882-6 2004 Overexpression of myr-Akt or Ran-wt in type II pneumocytes increased Akt ser-473 phosphorylation and mitosis in a catalase-dependent manner, indicating that H2O2 is essential for Akt and Ran signaling. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 22-25 15048882-6 2004 Overexpression of myr-Akt or Ran-wt in type II pneumocytes increased Akt ser-473 phosphorylation and mitosis in a catalase-dependent manner, indicating that H2O2 is essential for Akt and Ran signaling. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 69-72 15048882-6 2004 Overexpression of myr-Akt or Ran-wt in type II pneumocytes increased Akt ser-473 phosphorylation and mitosis in a catalase-dependent manner, indicating that H2O2 is essential for Akt and Ran signaling. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 69-72 14660586-3 2004 Here, we showed that AdE4(+), but not AdE4(-) vectors, selectively stimulated phosphorylation of both Akt at Ser(473) and Src kinase in ECs. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 102-105 15056278-8 2004 Serine phosphorylation of Akt, downstream of Shc activation, was also p75(NTR)-dependent. Serine 0-6 AKT serine/threonine kinase 1 Homo sapiens 26-29 15047842-6 2004 We have demonstrated that CVB3 infection leads to phosphorylation of PKB/Akt on both Ser-473 and Thr-308 residues through a PI3K-dependent mechanism. Serine 85-88 AKT serine/threonine kinase 1 Homo sapiens 73-76 14707121-8 2004 Thus, we propose that Ras-GAP interacts with Akt and is necessary for its activation, possibly via integrin-linked kinase-mediated phosphorylation of Ser-473. Serine 150-153 AKT serine/threonine kinase 1 Homo sapiens 45-48 15107404-4 2004 In this paper, we present evidence that the activation of the PI3K/Akt pathway in liver tumor cells blocks the growth inhibitory activity of C/EBPalpha through the PP2A-mediated dephosphorylation of C/EBPalpha on Ser 193, leading to a failure of C/EBPalpha to interact with and inhibit cdks and E2F. Serine 213-216 AKT serine/threonine kinase 1 Homo sapiens 67-70 14668339-5 2004 This activation is found in conjunction with an increased time-dependent phosphorylation of the downstream kinase AKT/protein kinase B on both Ser(473) and Thr(308) residues. Serine 143-146 AKT serine/threonine kinase 1 Homo sapiens 114-117 14982852-1 2004 A novel member of the human AMPK family, ARK5, was recently discovered to be a key molecule in mediating cancer cell migration activity in human pancreas cancer cell line PANC-1, and its activation was found to be induced by Akt-dependent phosphorylation at Ser 600. Serine 258-261 AKT serine/threonine kinase 1 Homo sapiens 225-228 14981538-2 2004 Activation of AKT is a complex process involving translocation to the plasma membrane and phosphorylation of serine and threonine amino-acid residues. Serine 109-115 AKT serine/threonine kinase 1 Homo sapiens 14-17 15068229-1 2004 BACKGROUND: Insulin increases endothelial nitric oxide (NO) production by activating endothelial nitric oxide synthase (eNOS) through protein kinase B (Akt)-mediated phosphorylation of serine residue 1179 (p-eNOS serine 1179). Serine 185-191 AKT serine/threonine kinase 1 Homo sapiens 152-155 15068229-1 2004 BACKGROUND: Insulin increases endothelial nitric oxide (NO) production by activating endothelial nitric oxide synthase (eNOS) through protein kinase B (Akt)-mediated phosphorylation of serine residue 1179 (p-eNOS serine 1179). Serine 213-219 AKT serine/threonine kinase 1 Homo sapiens 152-155 14679308-4 2004 VEGF induced phosphorylation of protein kinase B (PKB/Akt), a regulator of cell survival and apoptosis, on serine 473, but not on threonine 308, in an ILK-dependent manner. Serine 107-113 AKT serine/threonine kinase 1 Homo sapiens 50-57 14636889-0 2004 Akt/GSK3beta serine/threonine kinases: evidence for a signalling pathway mediated by familial Alzheimer"s disease mutations. Serine 13-19 AKT serine/threonine kinase 1 Homo sapiens 0-3 14636889-2 2004 Here, we report selective down regulation of the serine/threonine kinase, Akt/PKB, concurrent with elevated endogenous GSK3beta kinase activity in familial Alzheimer"s disease beta-amyloid precursor protein expressing human embryonic kidney (HEK) and familial Alzheimer"s disease presenilin lymphoblast cells. Serine 49-55 AKT serine/threonine kinase 1 Homo sapiens 74-81 14747535-5 2004 In this study, we found that LMP2A induced the phosphorylation of Akt (serine 473) in Burkitt"s lymphoma cell line Ramos and in gastric carcinoma cell line HSC-39 and partially enhanced cell viability following TGF-beta 1 treatment. Serine 71-77 AKT serine/threonine kinase 1 Homo sapiens 66-69 14767488-11 2004 Notably, phospho-Akt (Ser-473) was increased in FL (Bcl-2+ or -) (P<0.03) with increased phospho-Bad (Ser-136), as compared with levels in FH. Serine 22-25 AKT serine/threonine kinase 1 Homo sapiens 17-20 14767488-11 2004 Notably, phospho-Akt (Ser-473) was increased in FL (Bcl-2+ or -) (P<0.03) with increased phospho-Bad (Ser-136), as compared with levels in FH. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 17-20 14500571-5 2004 Akt and apoptosis signal-regulating kinase 1 (ASK1) were physically associated, and E2 induced the phosphorylation of ASK1 at serine-83, which is a consensus Akt phosphorylation site. Serine 126-132 AKT serine/threonine kinase 1 Homo sapiens 0-3 14500571-5 2004 Akt and apoptosis signal-regulating kinase 1 (ASK1) were physically associated, and E2 induced the phosphorylation of ASK1 at serine-83, which is a consensus Akt phosphorylation site. Serine 126-132 AKT serine/threonine kinase 1 Homo sapiens 158-161 14725621-3 2004 Indeed, upon IGF-1 activation, Akt phosphorylates polyQ-huntingtin at serine 421 and abrogates its toxicity. Serine 70-76 AKT serine/threonine kinase 1 Homo sapiens 31-34 12860993-7 2003 Dominant negative Akt adenovirus also inhibited only Ser-65 phosphorylation (3.7 +/- 0.1 versus 1.0 +/- 0.03). Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 18-21 14505491-5 2003 Akt/PKB phosphorylates Ron Ser-1394, thus providing a docking site for 14-3-3 (scaffold proteins binding to phosphoserine/phosphothreonine-containing sequences). Serine 27-30 AKT serine/threonine kinase 1 Homo sapiens 4-7 14603261-0 2003 Levels of PTEN protein modulate Akt phosphorylation on serine 473, but not on threonine 308, in IGF-II-overexpressing rhabdomyosarcomas cells. Serine 55-61 AKT serine/threonine kinase 1 Homo sapiens 32-35 14630392-3 2003 Here we report that the protein kinase PKB/Akt phosphorylates CDC25B on serine 353, resulting in a nuclear export-dependent cytoplasmic accumulation of the phosphatase. Serine 72-78 AKT serine/threonine kinase 1 Homo sapiens 39-42 14630392-3 2003 Here we report that the protein kinase PKB/Akt phosphorylates CDC25B on serine 353, resulting in a nuclear export-dependent cytoplasmic accumulation of the phosphatase. Serine 72-78 AKT serine/threonine kinase 1 Homo sapiens 43-46 14585966-0 2003 Akt phosphorylation of serine 21 on Pak1 modulates Nck binding and cell migration. Serine 23-29 AKT serine/threonine kinase 1 Homo sapiens 0-3 14585966-4 2003 The major site phosphorylated by Akt on Pak1 mapped to serine 21, a site originally shown to be weakly autophosphorylated on Pak1 when Cdc42 or Rac activates it. Serine 55-61 AKT serine/threonine kinase 1 Homo sapiens 33-36 14585966-4 2003 The major site phosphorylated by Akt on Pak1 mapped to serine 21, a site originally shown to be weakly autophosphorylated on Pak1 when Cdc42 or Rac activates it. Serine 55-61 AKT serine/threonine kinase 1 Homo sapiens 144-147 14585966-5 2003 A peptide derived from the region surrounding serine 21 was a substrate for Akt but not Pak1 in vitro, and Akt stimulated serine 21 phosphorylation on the full-length Pak1 much better than Rac did. Serine 46-52 AKT serine/threonine kinase 1 Homo sapiens 76-79 14585966-5 2003 A peptide derived from the region surrounding serine 21 was a substrate for Akt but not Pak1 in vitro, and Akt stimulated serine 21 phosphorylation on the full-length Pak1 much better than Rac did. Serine 46-52 AKT serine/threonine kinase 1 Homo sapiens 107-110 14585966-5 2003 A peptide derived from the region surrounding serine 21 was a substrate for Akt but not Pak1 in vitro, and Akt stimulated serine 21 phosphorylation on the full-length Pak1 much better than Rac did. Serine 46-52 AKT serine/threonine kinase 1 Homo sapiens 189-192 14585966-5 2003 A peptide derived from the region surrounding serine 21 was a substrate for Akt but not Pak1 in vitro, and Akt stimulated serine 21 phosphorylation on the full-length Pak1 much better than Rac did. Serine 122-128 AKT serine/threonine kinase 1 Homo sapiens 107-110 14727512-7 2003 Furthermore, sublethal ischemia gradually and persistently stimulated the phosphorylation of Akt-Ser-473 in the CA1 region after reperfusion. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 93-96 14727512-9 2003 Intracerebroventricular injection of wortmannin before preconditioning blocked both the increased in Akt-Ser-473 phosphorylation and the neuroprotective action of preconditioning. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 101-104 12900420-1 2003 Glycogen synthase kinase-3beta (GSK-3beta) activity is suppressed when it becomes phosphorylated on serine 9 by protein kinase B (Akt). Serine 100-106 AKT serine/threonine kinase 1 Homo sapiens 130-133 12867426-3 2003 During mitogenic sufficiency, the phosphoinositide 3-kinase (PI3K)/Akt pathway phosphorylates tuberin on Ser-939 and Thr-1462 that inhibits the tumor suppressor function of the TSC complex. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 67-70 12704229-7 2003 We now find that Akt activates the oxidase in vitro by phosphorylating serines S304 and S328 of p47(PHOX). Serine 71-78 AKT serine/threonine kinase 1 Homo sapiens 17-20 12911635-2 2003 We report that the phosphorylation of Akt, ERK, JNK and p38 kinases, whose activities depend on serine, threonine and tyrosine phosphorylation, were all increased during OGD. Serine 96-102 AKT serine/threonine kinase 1 Homo sapiens 38-41 12740362-4 2003 The present study shows that Akt phosphorylated Hsp27 on Ser-82 in vitro and in intact cells, and phosphorylation of Hsp27 resulted in its dissociation from Akt. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 29-32 12691603-2 2003 In the present study, we examined heregulin (HRG)-induced signal transduction of ErbB4 receptor and found that the phosphatidylinositol 3"-kinase (PI3K)-Akt pathway negatively regulated the extracellular signal-regulated kinase (ERK) cascade by phosphorylating Raf-1 on Ser(259). Serine 270-273 AKT serine/threonine kinase 1 Homo sapiens 153-156 12869408-0 2003 Increased phospho-AKT (Ser(473)) expression in bronchial dysplasia: implications for lung cancer prevention studies. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 18-21 12869408-2 2003 We performed immunohistochemical analysis of activated AKT [phosphorylated (p)-AKT Ser(473)] in tissue specimens of normal bronchial epithelium, bronchial hyperplasia and squamous metaplasia ("reactive" epithelium), bronchial dysplasia, and NSCLC. Serine 83-86 AKT serine/threonine kinase 1 Homo sapiens 55-58 12734380-8 2003 Mutation analysis indicated that 2 aa residues, Ser(304) and Ser(328), were phosphorylated by Akt. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 94-97 12734380-8 2003 Mutation analysis indicated that 2 aa residues, Ser(304) and Ser(328), were phosphorylated by Akt. Serine 61-64 AKT serine/threonine kinase 1 Homo sapiens 94-97 12764143-10 2003 At that time, Akt was no longer present or active, which resulted in a decrease in the inhibiting phosphorylation of GSK3beta on Ser-9 and hence in an increased GSK3beta activity. Serine 129-132 AKT serine/threonine kinase 1 Homo sapiens 14-17 12839924-6 2003 In addition, AKT was found to be activated by HBx, as evidenced by not only the phosphorylation of AKT at serine 473 but by the phosphorylation of the exogenous substrate histone H2B as well, and these were specifically blocked by the presence of wortmannin. Serine 106-112 AKT serine/threonine kinase 1 Homo sapiens 13-16 12839924-6 2003 In addition, AKT was found to be activated by HBx, as evidenced by not only the phosphorylation of AKT at serine 473 but by the phosphorylation of the exogenous substrate histone H2B as well, and these were specifically blocked by the presence of wortmannin. Serine 106-112 AKT serine/threonine kinase 1 Homo sapiens 99-102 12808093-1 2003 The antiapoptotic protein PED/PEA-15 features an Akt phosphorylation motif upstream from Ser(116). Serine 89-92 AKT serine/threonine kinase 1 Homo sapiens 49-52 12808093-3 2003 Based on Western blotting with specific phospho-Ser(116) PED/PEA-15 antibodies, Akt phosphorylation of PED/PEA-15 occurred mainly at Ser(116). Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 80-83 12808093-3 2003 Based on Western blotting with specific phospho-Ser(116) PED/PEA-15 antibodies, Akt phosphorylation of PED/PEA-15 occurred mainly at Ser(116). Serine 133-136 AKT serine/threonine kinase 1 Homo sapiens 80-83 12808093-4 2003 In addition, a mutant of PED/PEA-15 featuring the substitution of Ser(116)-->Gly (PED(S116-->G)) showed 10-fold-decreased phosphorylation by Akt. Serine 66-69 AKT serine/threonine kinase 1 Homo sapiens 147-150 12808093-5 2003 In intact 293 cells, Akt also induced phosphorylation of PED/PEA-15 at Ser(116). Serine 71-74 AKT serine/threonine kinase 1 Homo sapiens 21-24 12686550-2 2003 PKB/Akt activation is regulated by phosphoinositide phospholipid-mediated plasma membrane anchoring and by phosphorylation on Thr-308 and Ser-473. Serine 138-141 AKT serine/threonine kinase 1 Homo sapiens 0-3 12686550-2 2003 PKB/Akt activation is regulated by phosphoinositide phospholipid-mediated plasma membrane anchoring and by phosphorylation on Thr-308 and Ser-473. Serine 138-141 AKT serine/threonine kinase 1 Homo sapiens 4-7 12686550-4 2003 The integrin-linked kinase (ILK) is a potential regulator of phosphorylation of PKB/Akt on Ser-473. Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 80-83 12686550-4 2003 The integrin-linked kinase (ILK) is a potential regulator of phosphorylation of PKB/Akt on Ser-473. Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 84-87 12686550-7 2003 The inhibition of PKB/Akt Ser-473 phosphorylation was rescued by kinase-active ILK but not by a kinase-deficient mutant of ILK, suggesting a role for the kinase activity of ILK in the stimulation of PKB/Akt phosphorylation. Serine 26-29 AKT serine/threonine kinase 1 Homo sapiens 18-21 12686550-7 2003 The inhibition of PKB/Akt Ser-473 phosphorylation was rescued by kinase-active ILK but not by a kinase-deficient mutant of ILK, suggesting a role for the kinase activity of ILK in the stimulation of PKB/Akt phosphorylation. Serine 26-29 AKT serine/threonine kinase 1 Homo sapiens 22-25 12686550-7 2003 The inhibition of PKB/Akt Ser-473 phosphorylation was rescued by kinase-active ILK but not by a kinase-deficient mutant of ILK, suggesting a role for the kinase activity of ILK in the stimulation of PKB/Akt phosphorylation. Serine 26-29 AKT serine/threonine kinase 1 Homo sapiens 199-202 12686550-7 2003 The inhibition of PKB/Akt Ser-473 phosphorylation was rescued by kinase-active ILK but not by a kinase-deficient mutant of ILK, suggesting a role for the kinase activity of ILK in the stimulation of PKB/Akt phosphorylation. Serine 26-29 AKT serine/threonine kinase 1 Homo sapiens 203-206 12682057-4 2003 Utilizing the advantages provided by a cell-free methodology, we characterized phosphoinositide-dependent kinase 2 (PDK2), the putative kinase responsible for phosphorylating Akt on Ser-473. Serine 182-185 AKT serine/threonine kinase 1 Homo sapiens 175-178 12682057-5 2003 Immunodepleting cytosolic PDK1 from an in vitro reaction containing plasma membrane and cytosol markedly inhibited insulin-stimulated phosphorylation of Akt at the PDK1 site (Thr-308) but had no effect on phosphorylation at the PDK2 site (Ser-473). Serine 239-242 AKT serine/threonine kinase 1 Homo sapiens 153-156 12682057-7 2003 Akt isoforms 1-3 and a kinase-dead Akt1 (K179A) mutant were phosphorylated in a phosphatidylinositol 3,4,5-trisphosphate-dependent manner at Ser-473 in an in vitro reaction containing this novel adipocyte subcellular fraction. Serine 141-144 AKT serine/threonine kinase 1 Homo sapiens 0-3 12682057-7 2003 Akt isoforms 1-3 and a kinase-dead Akt1 (K179A) mutant were phosphorylated in a phosphatidylinositol 3,4,5-trisphosphate-dependent manner at Ser-473 in an in vitro reaction containing this novel adipocyte subcellular fraction. Serine 141-144 AKT serine/threonine kinase 1 Homo sapiens 35-39 12591925-3 2003 Mutation of serines 116 and 617 to alanine promoted a greater protein-protein interaction with hsp90 and Akt and greater phosphorylation on serine 1179, the major site for Akt phosphorylation. Serine 12-19 AKT serine/threonine kinase 1 Homo sapiens 105-108 12591925-3 2003 Mutation of serines 116 and 617 to alanine promoted a greater protein-protein interaction with hsp90 and Akt and greater phosphorylation on serine 1179, the major site for Akt phosphorylation. Serine 12-19 AKT serine/threonine kinase 1 Homo sapiens 172-175 12591925-3 2003 Mutation of serines 116 and 617 to alanine promoted a greater protein-protein interaction with hsp90 and Akt and greater phosphorylation on serine 1179, the major site for Akt phosphorylation. Serine 12-18 AKT serine/threonine kinase 1 Homo sapiens 105-108 12591925-3 2003 Mutation of serines 116 and 617 to alanine promoted a greater protein-protein interaction with hsp90 and Akt and greater phosphorylation on serine 1179, the major site for Akt phosphorylation. Serine 12-18 AKT serine/threonine kinase 1 Homo sapiens 172-175 12644590-4 2003 We report here that eNOS activation in response to TNF-alpha correlated with phosphorylation of Akt at Ser 473 and of eNOS itself at Ser 1179. Serine 103-106 AKT serine/threonine kinase 1 Homo sapiens 96-99 12556562-3 2003 The resistant clone displayed an activated phosphoinositide 3-kinase (PI3K)/AKT1 pathway, with levels of phosphatidylinositol (3,4,5) trisphosphate higher than the parental cells and increased levels of both Thr 308 and Ser 473 phosphorylated AKT1. Serine 220-223 AKT serine/threonine kinase 1 Homo sapiens 76-80 12649200-3 2003 Apoptosis assays and Western blot analyses revealed that apoptosis resistance to TRAIL correlates well with the level of Akt phosphorylation at Ser 473 rather than protein expression levels of TRAIL receptors, DR4 and DR5. Serine 144-147 AKT serine/threonine kinase 1 Homo sapiens 121-124 12458207-8 2003 AKT phosphorylates MLK3 on serine 674 both in vitro and in vivo. Serine 27-33 AKT serine/threonine kinase 1 Homo sapiens 0-3 12458207-9 2003 Furthermore, the expression of activated AKT1 inhibits MLK3-mediated cell death in a manner dependent on serine 674 phosphorylation. Serine 105-111 AKT serine/threonine kinase 1 Homo sapiens 41-45 12409306-3 2003 It has a putative Akt phosphorylation motif at amino acids 595-600, and Ser(600) was found to be phosphorylated by active Akt resulting in the activation of kinase activity toward the SAMS peptide, a consensus AMPK substrate. Serine 72-75 AKT serine/threonine kinase 1 Homo sapiens 18-21 12409306-3 2003 It has a putative Akt phosphorylation motif at amino acids 595-600, and Ser(600) was found to be phosphorylated by active Akt resulting in the activation of kinase activity toward the SAMS peptide, a consensus AMPK substrate. Serine 72-75 AKT serine/threonine kinase 1 Homo sapiens 122-125 12535517-2 2003 One protein that associates with 14-3-3 in an Akt-dependent manner is shown here to be the Yes-associated protein (YAP), which is phosphorylated by Akt at serine 127, leading to binding to 14-3-3. Serine 155-161 AKT serine/threonine kinase 1 Homo sapiens 46-49 12535517-2 2003 One protein that associates with 14-3-3 in an Akt-dependent manner is shown here to be the Yes-associated protein (YAP), which is phosphorylated by Akt at serine 127, leading to binding to 14-3-3. Serine 155-161 AKT serine/threonine kinase 1 Homo sapiens 148-151 12473596-6 2002 RESULTS: LY294002 suppressed growth and decreased expression of Akt (Ser(473)) expression in cancer cells. Serine 69-72 AKT serine/threonine kinase 1 Homo sapiens 64-67 12434148-1 2002 The protein kinase Akt/PKB is stimulated by the phosphorylation of two regulatory residues, Thr 309 of the activation segment and Ser 474 of the hydrophobic motif (HM), that are structurally and functionally conserved within the AGC kinase family. Serine 130-133 AKT serine/threonine kinase 1 Homo sapiens 19-22 12429987-2 2002 Akt activation requires the phosphorylation of both Thr-308 and Ser-473. Serine 64-67 AKT serine/threonine kinase 1 Homo sapiens 0-3 12446767-2 2002 It can be activated by protein kinase B (PKB)/Akt via phosphorylation at Ser-1177. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 41-44 12446767-2 2002 It can be activated by protein kinase B (PKB)/Akt via phosphorylation at Ser-1177. Serine 73-76 AKT serine/threonine kinase 1 Homo sapiens 46-49 12434148-1 2002 The protein kinase Akt/PKB is stimulated by the phosphorylation of two regulatory residues, Thr 309 of the activation segment and Ser 474 of the hydrophobic motif (HM), that are structurally and functionally conserved within the AGC kinase family. Serine 130-133 AKT serine/threonine kinase 1 Homo sapiens 23-26 12434148-4 2002 Comparison with the inactive PKB structure indicates that the role of Ser 474 phosphorylation is to promote the engagement of the HM with the N-lobe of the kinase domain, promoting a disorder-to-order transition of the alphaC helix. Serine 70-73 AKT serine/threonine kinase 1 Homo sapiens 29-32 12504922-2 2002 Treatment with sodium valproate caused a gradual but relatively large increase in the activation-associated phosphorylation of Akt on Ser-473, and a similarly gradual but more modest increase in the inhibition-associated phosphorylation of GSK3beta on Ser-9. Serine 134-137 AKT serine/threonine kinase 1 Homo sapiens 127-130 12351701-3 2002 Overexpression of wild-type SHIP2 (WT-SHIP2) inhibited insulin-induced phosphorylation of Akt at both Thr(308) and Ser(473) in Rat1 fibroblasts expressing insulin receptors. Serine 115-118 AKT serine/threonine kinase 1 Homo sapiens 90-93 12504922-5 2002 These results identify novel effects of sodium valproate on the Akt/GSK3beta signaling pathway, indicating that histone deacetylase inhibition is linked to activation of Akt, and show that two anti-bipolar agents have a common action, the increased inhibitory phosphorylation of Ser-9-GSK3beta. Serine 279-282 AKT serine/threonine kinase 1 Homo sapiens 64-67 12438236-9 2002 We also found constitutive phosphorylation of AKT at the Ser-473 site in TrkB transfectants, whereas there was only a minimal level of constitutive phosphorylation of AKT in SY5Y cells. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 46-49 12171932-4 2002 PDGF-BB stimulated Akt phosphorylation predominantly at Ser-473. Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 19-22 12374740-1 2002 The growth factor-activated AGC protein kinases RSK, S6K, PKB, MSK and SGK are activated by serine/threonine phosphorylation in the activation loop and in the hydrophobic motif, C-terminal to the kinase domain. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 58-61 12149249-2 2002 The paradigm for activation of Akt involves phosphatidylinositol 3-kinase-dependent membrane localization followed by activating phosphorylation of Thr-308 and Ser-473. Serine 160-163 AKT serine/threonine kinase 1 Homo sapiens 31-34 12244301-8 2002 Expression of Ser(473) phospho Akt in primary human breast cancers statistically correlated with expression of p27 in tumor cytosol. Serine 14-17 AKT serine/threonine kinase 1 Homo sapiens 31-34 12087097-6 2002 The phosphorylation of BAD at Ser-136 was blocked by treatment with wortmannin or cotransfection with the dominant negative Akt. Serine 30-33 AKT serine/threonine kinase 1 Homo sapiens 124-127 12138086-6 2002 EGF and/or insulin increased protein kinase B/Akt serine phosphorylation to a similar extent, whereas mitogen-activated protein kinase phosphorylation was more pronounced for EGF than for insulin. Serine 50-56 AKT serine/threonine kinase 1 Homo sapiens 46-49 12138086-7 2002 The impaired insulin-stimulated downstream signaling, measured as protein kinase B/Akt serine phosphorylation, in insulin-resistant cells (Type 2 diabetes) was improved by the addition of EGF. Serine 87-93 AKT serine/threonine kinase 1 Homo sapiens 83-86 12087097-10 2002 Moreover, because paclitaxel-induced apoptosis is mediated by activated Raf-1 and the region surrounding Ser-259 in Raf-1 conforms to a consensus sequence for phosphorylation by Akt, the regulation of Raf-1 by Akt was examined. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 178-181 12087097-10 2002 Moreover, because paclitaxel-induced apoptosis is mediated by activated Raf-1 and the region surrounding Ser-259 in Raf-1 conforms to a consensus sequence for phosphorylation by Akt, the regulation of Raf-1 by Akt was examined. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 210-213 12095980-8 2002 N-cadherin homophilic ligation initiates phosphatidylinositol 3-kinase-dependent activation of Akt resulting in Akt phosphorylation of Bad on serine 136. Serine 142-148 AKT serine/threonine kinase 1 Homo sapiens 95-98 12087097-11 2002 We demonstrated an association between Akt and Raf-1 and showed that the phosphorylation of Raf-1 on Ser-259 induced by paclitaxel was blocked by treatment with wortmannin or LY294002. Serine 101-104 AKT serine/threonine kinase 1 Homo sapiens 39-42 12095980-8 2002 N-cadherin homophilic ligation initiates phosphatidylinositol 3-kinase-dependent activation of Akt resulting in Akt phosphorylation of Bad on serine 136. Serine 142-148 AKT serine/threonine kinase 1 Homo sapiens 112-115 12006586-7 2002 The enhanced serine kinase specifically phosphorylates the conserved Ser(789) residue in IRS-1, which is in a sequence motif separate from the ones for MAPK, c-Jun N-terminal kinase, glycogen-synthase kinase 3 (GSK-3), Akt, phosphatidylinositol 3"-kinase, or casein kinase. Serine 69-72 AKT serine/threonine kinase 1 Homo sapiens 219-222 12208782-9 2002 The phosphorylation of Akt at Ser(473) and Thr(308) was significantly decreased following CEES treatment, reflecting the suppressed kinase activity of both PDK1 and PDK2. Serine 30-33 AKT serine/threonine kinase 1 Homo sapiens 23-26 12048182-4 2002 High doses of insulin-like growth factor I (IGF-I) activate Akt quickly and strongly enough to suppress Raf kinase activity via phosphorylation of Ser-259, whereas low doses of IGF-I do not trigger this cross-talk but are still mitogenic. Serine 147-150 AKT serine/threonine kinase 1 Homo sapiens 60-63 12042314-8 2002 Although Akt also phosphorylated p27(Kip1) at Ser(10) and Thr(187), these two sites were not involved in the binding to 14-3-3 proteins. Serine 46-49 AKT serine/threonine kinase 1 Homo sapiens 9-12 12000750-4 2002 This effect was correlated with inhibition of the phosphorylation of the PDK1 downstream substrate Akt/protein kinase B (PKB) on two regulatory sites, Thr(308) and Ser(473). Serine 164-167 AKT serine/threonine kinase 1 Homo sapiens 99-102 12000750-4 2002 This effect was correlated with inhibition of the phosphorylation of the PDK1 downstream substrate Akt/protein kinase B (PKB) on two regulatory sites, Thr(308) and Ser(473). Serine 164-167 AKT serine/threonine kinase 1 Homo sapiens 121-124 12138205-7 2002 The regulation of NF-kappa B-dependent transcription by Cot requires Akt-dependent phosphorylation of serine 400 (S400), near the carboxy terminus of Cot. Serine 102-108 AKT serine/threonine kinase 1 Homo sapiens 69-72 12060641-4 2002 The expression of phosphorylated Akt (Ser(473)) and apoptosis in cancer cells were determined by immunoblotting and immunohistochemistry. Serine 38-41 AKT serine/threonine kinase 1 Homo sapiens 33-36 12000752-7 2002 RA treatment activates the PI3K/Akt signaling pathway, resulting in increased PI3K activity in extracts from RA-treated cells and a rapid increase in phosphorylation of Akt in Ser-473. Serine 176-179 AKT serine/threonine kinase 1 Homo sapiens 32-35 12000752-7 2002 RA treatment activates the PI3K/Akt signaling pathway, resulting in increased PI3K activity in extracts from RA-treated cells and a rapid increase in phosphorylation of Akt in Ser-473. Serine 176-179 AKT serine/threonine kinase 1 Homo sapiens 169-172 12164932-6 2002 Both serine-473 and threonine-308 sites of protein kinase B/Akt show increased phosphorylation in ganglioside-deficient cells, but the cell survival correlates with increased phosphorylation of the serine-473 site of Akt, not with increased phosphorylation of the threonine-308 site. Serine 5-11 AKT serine/threonine kinase 1 Homo sapiens 60-63 12164932-6 2002 Both serine-473 and threonine-308 sites of protein kinase B/Akt show increased phosphorylation in ganglioside-deficient cells, but the cell survival correlates with increased phosphorylation of the serine-473 site of Akt, not with increased phosphorylation of the threonine-308 site. Serine 198-204 AKT serine/threonine kinase 1 Homo sapiens 60-63 12164932-6 2002 Both serine-473 and threonine-308 sites of protein kinase B/Akt show increased phosphorylation in ganglioside-deficient cells, but the cell survival correlates with increased phosphorylation of the serine-473 site of Akt, not with increased phosphorylation of the threonine-308 site. Serine 198-204 AKT serine/threonine kinase 1 Homo sapiens 217-220 12164932-7 2002 Consistently, blockade of ganglioside GT1b function activates integrin-linked kinase and only the serine-473 site of protein kinase B/Akt. Serine 98-104 AKT serine/threonine kinase 1 Homo sapiens 134-137 11956222-10 2002 These data indicate that PKB/Akt phosphorylates Ser-58 on 14-3-3zeta both in vitro and in intact cells. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 25-28 11956222-10 2002 These data indicate that PKB/Akt phosphorylates Ser-58 on 14-3-3zeta both in vitro and in intact cells. Serine 48-51 AKT serine/threonine kinase 1 Homo sapiens 29-32 12060641-6 2002 RESULTS: LY294002 demonstrated a remarkable growth-inhibitory and apoptosis-inducing effect in these colon cancer cell lines, with decreased expression of phosphorylated Akt (Ser(473)). Serine 175-178 AKT serine/threonine kinase 1 Homo sapiens 170-173 11809767-4 2002 Phosphorylation studies of the C-terminal cytoplasmic domain of the beta(2)-adrenergic receptor by Akt in vitro identified Ser(345) and Ser(346) within a consensus motif for Akt phosphorylation. Serine 123-126 AKT serine/threonine kinase 1 Homo sapiens 99-102 11809746-5 2002 Insulin-stimulated serine phosphorylation of Akt/protein kinase B was significantly impaired in mutant-transfected cells. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 45-48 12062094-1 2002 In the search for neuroprotective factors in Huntington"s disease, we found that insulin growth factor 1 via activation of the serine/threonine kinase Akt/PKB is able to inhibit neuronal death specifically induced by mutant huntingtin containing an expanded polyglutamine stretch. Serine 127-133 AKT serine/threonine kinase 1 Homo sapiens 151-154 12062094-1 2002 In the search for neuroprotective factors in Huntington"s disease, we found that insulin growth factor 1 via activation of the serine/threonine kinase Akt/PKB is able to inhibit neuronal death specifically induced by mutant huntingtin containing an expanded polyglutamine stretch. Serine 127-133 AKT serine/threonine kinase 1 Homo sapiens 155-158 11809767-4 2002 Phosphorylation studies of the C-terminal cytoplasmic domain of the beta(2)-adrenergic receptor by Akt in vitro identified Ser(345) and Ser(346) within a consensus motif for Akt phosphorylation. Serine 123-126 AKT serine/threonine kinase 1 Homo sapiens 174-177 11809767-4 2002 Phosphorylation studies of the C-terminal cytoplasmic domain of the beta(2)-adrenergic receptor by Akt in vitro identified Ser(345) and Ser(346) within a consensus motif for Akt phosphorylation. Serine 136-139 AKT serine/threonine kinase 1 Homo sapiens 99-102 11809767-4 2002 Phosphorylation studies of the C-terminal cytoplasmic domain of the beta(2)-adrenergic receptor by Akt in vitro identified Ser(345) and Ser(346) within a consensus motif for Akt phosphorylation. Serine 136-139 AKT serine/threonine kinase 1 Homo sapiens 174-177 11756412-6 2002 Two sites (Thr(145) and Ser(146)) in the carboxyl terminus of p21 are phosphorylated by AKT/PKB in vitro and in vivo. Serine 24-27 AKT serine/threonine kinase 1 Homo sapiens 88-91 11756412-6 2002 Two sites (Thr(145) and Ser(146)) in the carboxyl terminus of p21 are phosphorylated by AKT/PKB in vitro and in vivo. Serine 24-27 AKT serine/threonine kinase 1 Homo sapiens 92-95 11855836-3 2002 FANCA has a consensus sequence for Akt kinase near serine 1149 (Ser1149), suggesting that Akt can phosphorylate FANCA. Serine 51-57 AKT serine/threonine kinase 1 Homo sapiens 35-38 11960368-4 2002 This association was concurrent with phosphorylation of Akt (at Ser 473), and resulted in elevated expression of HDM2 and enhanced nuclear localization. Serine 64-67 AKT serine/threonine kinase 1 Homo sapiens 56-59 11960368-5 2002 However, analysis of HDM2 proteins mutated at the consensus Akt recognition sites at serines 166 and 186 indicated that modification at these residues was not sufficient for the increased expression of the protein, which was blocked by the PI3 kinase inhibitor LY294002. Serine 85-92 AKT serine/threonine kinase 1 Homo sapiens 60-63 11855836-3 2002 FANCA has a consensus sequence for Akt kinase near serine 1149 (Ser1149), suggesting that Akt can phosphorylate FANCA. Serine 51-57 AKT serine/threonine kinase 1 Homo sapiens 90-93 11850850-5 2002 On the other hand upon serum stimulation, when Akt becomes active and enhances cell survival, phosphorylation occurs at an Akt consensus site (serine 166) within the Mdm2 protein, a key regulator of p53 function. Serine 143-149 AKT serine/threonine kinase 1 Homo sapiens 47-50 11804874-5 2002 Moreover, these stimuli appeared to induce the serine and threonine phosphorylation of Akt, which was reduced by the PI 3-kinase inhibitor LY294002. Serine 47-53 AKT serine/threonine kinase 1 Homo sapiens 87-90 11850850-5 2002 On the other hand upon serum stimulation, when Akt becomes active and enhances cell survival, phosphorylation occurs at an Akt consensus site (serine 166) within the Mdm2 protein, a key regulator of p53 function. Serine 143-149 AKT serine/threonine kinase 1 Homo sapiens 123-126 11707464-7 2002 In vitro and in vivo (32)P labeling indicated that Akt phosphorylated SEK1 on serine 78. Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 51-54 12503617-5 2002 Interleukin-4 stimulation induced mono-phosphorylation at serine residue S473 of Akt kinase but failed to activate the kinase. Serine 58-64 AKT serine/threonine kinase 1 Homo sapiens 81-84 11826414-8 2002 In conclusion, these findings show that in PHT gastric mucosa, TNF-alpha stimulates eNOS phosphorylation at serine 1177 (required for its activation) via the PI 3-kinase-Akt signal transduction pathway. Serine 108-114 AKT serine/threonine kinase 1 Homo sapiens 170-173 11818508-8 2002 Phosphorylation of Akt on threonine-308 was more sensitive to Ca(2+) depletion than phosphorylation of Akt on serine-473 at the same insulin dose (10 nM). Serine 110-116 AKT serine/threonine kinase 1 Homo sapiens 103-106 11723060-6 2001 Insulin increased Akt serine phosphorylation in control subjects and IGT relatives, with a tendency for reduced phosphorylation in IGT relatives (P = 0.12). Serine 22-28 AKT serine/threonine kinase 1 Homo sapiens 18-21 12210737-0 2002 Flow cytometric detection of total and serine 473 phosphorylated Akt. Serine 39-45 AKT serine/threonine kinase 1 Homo sapiens 65-68 12210737-3 2002 For its full activation Akt needs to be phosphorylated on Serine 473 residue. Serine 58-64 AKT serine/threonine kinase 1 Homo sapiens 24-27 11726183-0 2001 Development and validation of a competitive AKT serine/threonine kinase fluorescence polarization assay using a product-specific anti-phospho-serine antibody. Serine 48-54 AKT serine/threonine kinase 1 Homo sapiens 44-47 11756242-10 2002 Furthermore, utilizing an antibody that specifically recognizes the serine-473 phosphorylated form of AKT, we observed that activated AKT was detectable in the neoplastic but not normal epithelium. Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 102-105 11756242-10 2002 Furthermore, utilizing an antibody that specifically recognizes the serine-473 phosphorylated form of AKT, we observed that activated AKT was detectable in the neoplastic but not normal epithelium. Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 134-137 11756319-11 2002 Insulin-stimulated serine phosphorylation of Akt was augmented after troglitazone (170 +/- 34% of pre-Rx response, P < 0.05) treatment and unchanged by metformin. Serine 19-25 AKT serine/threonine kinase 1 Homo sapiens 45-48 11777539-4 2002 In contrast to IL-10, IGF-I increases enzymatic activity of PI 3-kinase and causes phosphorylation on serine(473) of Akt but does not prevent microglial apoptosis. Serine 102-108 AKT serine/threonine kinase 1 Homo sapiens 117-120 11731414-7 2001 Our results showed that estrogen stimulated Akt activation, as indicated by phosphorylation at Ser(473) of the oncoprotein, in ER-negative breast cancer cells. Serine 95-98 AKT serine/threonine kinase 1 Homo sapiens 44-47 11577096-5 2001 Consistently, blockade of GT1b function with anti-GT1b antibody specifically activated the Ser-473 site of Akt, markedly suppressing apoptosis. Serine 91-94 AKT serine/threonine kinase 1 Homo sapiens 107-110 11546775-8 2001 Akt phosphorylation at Thr-308 increased 4-fold and at Ser-473 1.4-fold within 10 min after decorin addition. Serine 55-58 AKT serine/threonine kinase 1 Homo sapiens 0-3 11579209-0 2001 Phosphorylation of PTP1B at Ser(50) by Akt impairs its ability to dephosphorylate the insulin receptor. Serine 28-31 AKT serine/threonine kinase 1 Homo sapiens 39-42 11679435-6 2001 C2-ceramide completely abolished insulin-stimulated Akt/protein kinase B phosphorylation on regulatory residues Thr 308 and Ser 473, as did TNF-alpha, and inhibited insulin-induced mobility shift in Akt1 and Akt2 separated in PAGE. Serine 124-127 AKT serine/threonine kinase 1 Homo sapiens 52-55 11696579-1 2001 Endothelial nitric oxide synthase (eNOS) is activated by phosphorylation of serine 1177 by the protein kinase Akt/PKB. Serine 76-82 AKT serine/threonine kinase 1 Homo sapiens 110-117 11602740-10 2001 PI-3K activates Akt by phosphorylation of threonine 308 and serine 473. Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 16-19 11711890-2 2001 Phosphorylation of Akt (serine-473) was enhanced in cortex after 1-hour ischemia, and also after 1h and 6 h of reperfusion, but it returned back to that in controls by 24 h. After this first wave of Akt activation, a second increase was observed between 4 and 7 days. Serine 24-30 AKT serine/threonine kinase 1 Homo sapiens 19-22 11711890-2 2001 Phosphorylation of Akt (serine-473) was enhanced in cortex after 1-hour ischemia, and also after 1h and 6 h of reperfusion, but it returned back to that in controls by 24 h. After this first wave of Akt activation, a second increase was observed between 4 and 7 days. Serine 24-30 AKT serine/threonine kinase 1 Homo sapiens 199-202 11598301-5 2001 Binding of CTMP reduces the activity of PKBalpha by inhibiting phosphorylation on serine 473 and threonine 308. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 40-48 11579209-3 2001 Interestingly, PTP1B contains this motif (RYRDVS(50)), and wild-type PTP1B (but not mutants with substitutions for Ser(50)) was significantly phosphorylated by Akt in vitro. Serine 115-118 AKT serine/threonine kinase 1 Homo sapiens 160-163 11579209-14 2001 We conclude that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling. Serine 96-99 AKT serine/threonine kinase 1 Homo sapiens 48-51 11579209-14 2001 We conclude that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling. Serine 96-99 AKT serine/threonine kinase 1 Homo sapiens 89-92 11438544-2 2001 We show that phosphatidylinositol 3-kinase (PI3K) and its effector kinase Akt play an important role in the serine phosphorylation of STAT1 and in the activation of gene expression in response to interferon-gamma (IFN gamma). Serine 108-114 AKT serine/threonine kinase 1 Homo sapiens 74-77 11504915-3 2001 Mitogen-induced activation of phosphatidylinositol 3-kinase (PI3-kinase) and its downstream target, the Akt/PKB serine-threonine kinase, results in phosphorylation of Mdm2 on serine 166 and serine 186. Serine 112-118 AKT serine/threonine kinase 1 Homo sapiens 104-107 11504915-3 2001 Mitogen-induced activation of phosphatidylinositol 3-kinase (PI3-kinase) and its downstream target, the Akt/PKB serine-threonine kinase, results in phosphorylation of Mdm2 on serine 166 and serine 186. Serine 175-181 AKT serine/threonine kinase 1 Homo sapiens 104-107 11443134-8 2001 Moreover, Akt phosphorylated Raf at Ser(259), resulting in a reduced Raf kinase activity and a termination of MEK and ERK1/2 phosphorylation. Serine 36-39 AKT serine/threonine kinase 1 Homo sapiens 10-13 11438544-6 2001 In addition to PI3K and Akt, JAK1, JAK2, and the tyrosine 440 STAT1 docking residue of IFNGR1 are required for STAT1 to be phosphorylated on serine. Serine 141-147 AKT serine/threonine kinase 1 Homo sapiens 24-27 11438544-7 2001 Taken together, these results suggest that the following events lead to the activation of STAT1 upon IFN gamma stimulation: 1) PI3K and Akt are activated by the occupied receptor and Tyr-440 is phosphorylated by the activated JAKs; 2) STAT1 docks to Tyr-440; and 3) Tyr-701 is phosphorylated by the JAKs and Ser-727 is phosphorylated by a kinase downstream of Akt. Serine 308-311 AKT serine/threonine kinase 1 Homo sapiens 136-139 11438544-7 2001 Taken together, these results suggest that the following events lead to the activation of STAT1 upon IFN gamma stimulation: 1) PI3K and Akt are activated by the occupied receptor and Tyr-440 is phosphorylated by the activated JAKs; 2) STAT1 docks to Tyr-440; and 3) Tyr-701 is phosphorylated by the JAKs and Ser-727 is phosphorylated by a kinase downstream of Akt. Serine 308-311 AKT serine/threonine kinase 1 Homo sapiens 360-363 11313365-3 2001 The kinase responsible for phosphorylation of threonine 308 is the PI 3-kinase-dependent kinase-1 (PDK-1), whereas phosphorylation of serine 473 has been suggested to be regulated by PKB/Akt autophosphorylation in a PDK-1-dependent manner. Serine 134-140 AKT serine/threonine kinase 1 Homo sapiens 183-186 11425855-7 2001 Further experiments in transfected COS cells expressing either wild-type or S1177A mutant eNOS led us to identify the serine 1177 as the critical residue for the hsp90-dependent Akt-mediated activation of eNOS. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 178-181 11445557-2 2001 Phosphorylation of Thr(308) and Ser(473) of Akt by its upstream kinase(s) or autophosphorylation is critical for optimal activation of its kinase activity. Serine 32-35 AKT serine/threonine kinase 1 Homo sapiens 44-47 11313365-0 2001 Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343. Serine 35-41 AKT serine/threonine kinase 1 Homo sapiens 31-34 11313365-3 2001 The kinase responsible for phosphorylation of threonine 308 is the PI 3-kinase-dependent kinase-1 (PDK-1), whereas phosphorylation of serine 473 has been suggested to be regulated by PKB/Akt autophosphorylation in a PDK-1-dependent manner. Serine 134-140 AKT serine/threonine kinase 1 Homo sapiens 187-190 11313365-0 2001 Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343. Serine 157-163 AKT serine/threonine kinase 1 Homo sapiens 31-34 11313365-2 2001 To become fully activated, PKB/Akt requires phosphorylation at two sites, threonine 308 and serine 473, in a phosphatidylinositol (PI) 3-kinase-dependent manner. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 27-30 11313365-8 2001 The amino acid residue serine 343 of ILK within the activation loop is required for kinase activity as well as for its interaction with PKB/Akt. Serine 23-29 AKT serine/threonine kinase 1 Homo sapiens 136-139 11313365-2 2001 To become fully activated, PKB/Akt requires phosphorylation at two sites, threonine 308 and serine 473, in a phosphatidylinositol (PI) 3-kinase-dependent manner. Serine 92-98 AKT serine/threonine kinase 1 Homo sapiens 31-34 11313365-8 2001 The amino acid residue serine 343 of ILK within the activation loop is required for kinase activity as well as for its interaction with PKB/Akt. Serine 23-29 AKT serine/threonine kinase 1 Homo sapiens 140-143 11359783-7 2001 Arachidonic acid also transiently stimulated the phosphorylation of Akt on Thr-308 and Ser-473. Serine 87-90 AKT serine/threonine kinase 1 Homo sapiens 68-71 11313365-9 2001 Mutational analysis of ILK further shows a crucial role for arginine 211 of ILK within the phosphoinositide phospholipid binding domain in the regulation of PKB- serine 473 phosphorylation. Serine 162-168 AKT serine/threonine kinase 1 Homo sapiens 157-160 11278872-7 2001 Phosphorylation of Akt at serine 473 and Bad at serine 136 were induced by HBx, which were specifically blocked by wortmannin and dominant negative mutants of Akt and Bad, respectively. Serine 26-32 AKT serine/threonine kinase 1 Homo sapiens 19-22 11350959-7 2001 Instead, Akt was phosphorylated at both threonine 308 and serine 473 and activated by UVB-activated mitogen- and stress-activated protein kinase 1 (Msk1). Serine 58-64 AKT serine/threonine kinase 1 Homo sapiens 9-12 11373274-1 2001 Full activation of protein kinase B (PKB, also called Akt) requires phosphorylation on two regulatory sites, Thr-308 in the activation loop and Ser-473 in the hydrophobic C-terminal regulatory domain (numbering for PKB alpha/Akt-1). Serine 144-147 AKT serine/threonine kinase 1 Homo sapiens 54-57 11404460-2 2001 Here, we demonstrate that Akt phosphorylates the androgen receptor (AR) at Ser-210 and Ser-790. Serine 75-78 AKT serine/threonine kinase 1 Homo sapiens 26-29 11404460-2 2001 Here, we demonstrate that Akt phosphorylates the androgen receptor (AR) at Ser-210 and Ser-790. Serine 87-90 AKT serine/threonine kinase 1 Homo sapiens 26-29 11404460-3 2001 A mutation at AR Ser-210 results in the reversal of Akt-mediated suppression of AR transactivation. Serine 17-20 AKT serine/threonine kinase 1 Homo sapiens 52-55 11278872-7 2001 Phosphorylation of Akt at serine 473 and Bad at serine 136 were induced by HBx, which were specifically blocked by wortmannin and dominant negative mutants of Akt and Bad, respectively. Serine 26-32 AKT serine/threonine kinase 1 Homo sapiens 159-162 11278872-7 2001 Phosphorylation of Akt at serine 473 and Bad at serine 136 were induced by HBx, which were specifically blocked by wortmannin and dominant negative mutants of Akt and Bad, respectively. Serine 48-54 AKT serine/threonine kinase 1 Homo sapiens 19-22 11278872-7 2001 Phosphorylation of Akt at serine 473 and Bad at serine 136 were induced by HBx, which were specifically blocked by wortmannin and dominant negative mutants of Akt and Bad, respectively. Serine 48-54 AKT serine/threonine kinase 1 Homo sapiens 159-162 11313398-5 2001 Furthermore, FcalphaR cross-linking triggered recruitment of PDK1 and serine-phosphorylated PKBalpha to capped cell surface FcalphaR irrespective of the gamma-chain ITAM. Serine 70-76 AKT serine/threonine kinase 1 Homo sapiens 92-100 11292821-2 2001 Multiple protein kinases including Akt/PKB, cAMP-dependent protein kinase (PKA), and the AMP-activated protein kinase (AMPK) activate eNOS by phosphorylating Ser-1177 in response to various stimuli. Serine 158-161 AKT serine/threonine kinase 1 Homo sapiens 35-42 11313398-7 2001 In wt-ITAM cells, PDK1 and serine-phosphorylated PKBalpha translocated to lysosomal-associated membrane glycoprotein 1- and cathepsin B-containing vesicles, consistent with MHC class II peptide-loading compartments (MIIC) described by other groups. Serine 27-33 AKT serine/threonine kinase 1 Homo sapiens 49-57 11274386-5 2001 We further show that Akt specifically phosphorylates Ser-350 of the Nur77 protein within its DNA-binding domain in vitro and in vivo in 293 and NIH 3T3 cells. Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 21-24 11340092-12 2001 Zinc significantly increased serine-473 phosphorylation of Akt in both preadipocytes and adipocytes (P < 0.05). Serine 29-35 AKT serine/threonine kinase 1 Homo sapiens 59-62 11323521-4 2001 Sublethal ischemia gradually and persistently stimulated phosphorylation of Akt-Ser-473 in the hippocampal CA1 region after reperfusion. Serine 80-83 AKT serine/threonine kinase 1 Homo sapiens 76-79 11323521-5 2001 After lethal ischemia, phosphorylation of Akt-Ser-473 showed no obvious decrease in preconditioned gerbils but a marked decrease in nonconditioned gerbils. Serine 46-49 AKT serine/threonine kinase 1 Homo sapiens 42-45 11323521-6 2001 Changes in Akt-Ser-473 phosphorylation were correlated with changes in Akt activities, as measured by an in vitro kinase assay. Serine 15-18 AKT serine/threonine kinase 1 Homo sapiens 11-14 11323521-6 2001 Changes in Akt-Ser-473 phosphorylation were correlated with changes in Akt activities, as measured by an in vitro kinase assay. Serine 15-18 AKT serine/threonine kinase 1 Homo sapiens 71-74 11323521-7 2001 Intracerebral ventricular infusion of wortmannin before preconditioning blocked both the increase in Akt-Ser-473 phosphorylation in a dose-dependent manner and the neuroprotective action of preconditioning. Serine 105-108 AKT serine/threonine kinase 1 Homo sapiens 101-104 11312610-4 2001 The activation of Akt and MAPK in parallel pathways downstream from PI3K resulted in the phosphorylation of Bad at different serine residues. Serine 125-131 AKT serine/threonine kinase 1 Homo sapiens 18-21 11319202-2 2001 Stimulating ECs with vascular endothelial growth factor (VEGF) leads to the activation of Akt/protein kinase B, which in turn activates endothelial nitric oxide synthase (eNOS) by phosphorylation on serine 1177. Serine 199-205 AKT serine/threonine kinase 1 Homo sapiens 90-93 11278269-2 2001 Akt contains a pleckstrin homology (PH) domain and is activated by phosphorylation at threonine 308 and serine 473. Serine 104-110 AKT serine/threonine kinase 1 Homo sapiens 0-3 11278269-7 2001 cAMP potently inhibits phosphorylation at threonine 308 and serine 473 of Akt, which is required for the protein kinase activities of Akt. Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 74-77 11278269-7 2001 cAMP potently inhibits phosphorylation at threonine 308 and serine 473 of Akt, which is required for the protein kinase activities of Akt. Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 134-137 11254732-4 2001 We observed that LPS exposure results in phosphorylation of Akt (serine 473). Serine 65-71 AKT serine/threonine kinase 1 Homo sapiens 60-63 11254732-6 2001 AKT activation resulted in the phosphorylation-dependent inactivation of glycogen synthase kinase (GSK-3) (serine 21/9). Serine 107-113 AKT serine/threonine kinase 1 Homo sapiens 0-3 11139588-7 2001 The consensus AKT phosphorylation site Ser-167 of ERalpha is required for phosphorylation and activation by AKT. Serine 39-42 AKT serine/threonine kinase 1 Homo sapiens 14-17 11139588-7 2001 The consensus AKT phosphorylation site Ser-167 of ERalpha is required for phosphorylation and activation by AKT. Serine 39-42 AKT serine/threonine kinase 1 Homo sapiens 108-111 11274386-8 2001 Thus, we show that Akt interacts with Nur77 and inactivates Nur77 by phosphorylation at Ser-350 in a phosphatidylinositol 3-kinase-dependent manner, connecting the phosphatidylinositol 3-kinase-dependent Akt pathway and a nuclear receptor pathway. Serine 88-91 AKT serine/threonine kinase 1 Homo sapiens 19-22 11274386-8 2001 Thus, we show that Akt interacts with Nur77 and inactivates Nur77 by phosphorylation at Ser-350 in a phosphatidylinositol 3-kinase-dependent manner, connecting the phosphatidylinositol 3-kinase-dependent Akt pathway and a nuclear receptor pathway. Serine 88-91 AKT serine/threonine kinase 1 Homo sapiens 204-207 11042204-1 2001 Akt activation requires phosphorylation of Thr(308) and Ser(473) by 3-phosphoinositide-dependent kinase-1 and 2 (PDK1 and PDK2), respectively. Serine 56-59 AKT serine/threonine kinase 1 Homo sapiens 0-3 11289110-4 2001 Hypoxia-induced signaling also resulted in PI 3-K-dependent phosphorylation of Akt on Ser-473, a modification of Akt that is important for its activation. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 79-82 11289110-4 2001 Hypoxia-induced signaling also resulted in PI 3-K-dependent phosphorylation of Akt on Ser-473, a modification of Akt that is important for its activation. Serine 86-89 AKT serine/threonine kinase 1 Homo sapiens 113-116 11289110-7 2001 Activation of Akt by hypoxia resulted in the phosphorylation of GSK-3alpha and GSK-3beta at Ser-9 and Ser-21, two well-documented Akt phosphorylation sites, respectively, that are inactivating modifications of each GSK-3 isoform. Serine 92-95 AKT serine/threonine kinase 1 Homo sapiens 14-17 11289110-7 2001 Activation of Akt by hypoxia resulted in the phosphorylation of GSK-3alpha and GSK-3beta at Ser-9 and Ser-21, two well-documented Akt phosphorylation sites, respectively, that are inactivating modifications of each GSK-3 isoform. Serine 102-105 AKT serine/threonine kinase 1 Homo sapiens 14-17 11289110-7 2001 Activation of Akt by hypoxia resulted in the phosphorylation of GSK-3alpha and GSK-3beta at Ser-9 and Ser-21, two well-documented Akt phosphorylation sites, respectively, that are inactivating modifications of each GSK-3 isoform. Serine 102-105 AKT serine/threonine kinase 1 Homo sapiens 130-133 11042204-4 2001 Inhibition of p38 kinase activity with SB203580 inhibited Akt Ser(473) phosphorylation following neutrophil stimulation with formyl-methionyl-leucyl-phenylalanine, FcgammaR cross-linking, or phosphatidylinositol 3,4,5-trisphosphate. Serine 62-65 AKT serine/threonine kinase 1 Homo sapiens 58-61 11208609-7 2001 Furthermore, gp 120 also exhibited a dose-dependent inhibition of Akt phosphorylation at Ser-473 without any significant alteration of Akt expression. Serine 89-92 AKT serine/threonine kinase 1 Homo sapiens 66-69 11162641-1 2001 In the present study, treatment of the PTEN negative U87MG human glioblastoma cell line with C2-ceramide resulted in a dose- and time-dependent decrease in the constitutive phosphorylation of Akt at threonine 308 and serine 473. Serine 217-223 AKT serine/threonine kinase 1 Homo sapiens 192-195 11156964-3 2001 In blocking experiments performed with pharmacological inhibitors, Tat decreased the intracellular levels of cAMP and CREB Ser-133 phosphorylation through a signal transduction pathway involving the sequential activation of phosphatidylinositol 3-kinase, AKT, and cyclic nucleoside phosphodiesterases. Serine 123-126 AKT serine/threonine kinase 1 Homo sapiens 255-258 11162573-4 2001 Chronic TGZ treatment increased insulin-stimulated phosphatidylinositol (PI) 3-kinase activity and membranous protein kinase B/Akt (PKB/Akt) Ser-473 phosphorylation more than 4-fold. Serine 141-144 AKT serine/threonine kinase 1 Homo sapiens 110-139 11154276-3 2001 Akt decreased ASK1 kinase activity stimulated by both oxidative stress and overexpression in 293 cells by phosphorylating a consensus Akt site at serine 83 of ASK1. Serine 146-152 AKT serine/threonine kinase 1 Homo sapiens 0-3 11154276-3 2001 Akt decreased ASK1 kinase activity stimulated by both oxidative stress and overexpression in 293 cells by phosphorylating a consensus Akt site at serine 83 of ASK1. Serine 146-152 AKT serine/threonine kinase 1 Homo sapiens 134-137 11154276-7 2001 Finally, activation of the PI3-K/Akt pathway reduced apoptosis induced by ASK1 in a manner dependent on phosphorylation of serine 83 of ASK1. Serine 123-129 AKT serine/threonine kinase 1 Homo sapiens 33-36 11154281-10 2001 While both kinases can phosphorylate Thr-32, SGK displays a marked preference for Ser-315 whereas Akt favors Ser-253. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 98-101 11058583-0 2001 Rac/Cdc42 and p65PAK regulate the microtubule-destabilizing protein stathmin through phosphorylation at serine 16. Serine 104-110 AKT serine/threonine kinase 1 Homo sapiens 0-3 11752635-1 2001 The kinase Akt contains two phosphatidylinositol-3 kinase (PI3K)-dependent phosphorylation sites, one in the activation loop (Thr(308)) and one in the carboxyl-terminal tail (Ser(473)), both of which are conserved among the members of the AGC kinase family. Serine 175-178 AKT serine/threonine kinase 1 Homo sapiens 11-14 11752635-6 2001 This Perspective examines the regulation and biological significance of Akt phosphorylation at Ser(473). Serine 95-98 AKT serine/threonine kinase 1 Homo sapiens 72-75 11752635-9 2001 These interactions may induce conformational changes in Akt that make Ser(473) accessible to phosphorylation. Serine 70-73 AKT serine/threonine kinase 1 Homo sapiens 56-59 11108261-9 2000 Serines S104 S106, S118, and S167 appear to play a role in the activation of ER-alpha by Akt. Serine 0-7 AKT serine/threonine kinase 1 Homo sapiens 89-92 11156376-4 2000 Akt specifically binds to AR and phosphorylates serines 213 and 791 of AR. Serine 48-55 AKT serine/threonine kinase 1 Homo sapiens 0-3 10986289-3 2000 In HEK-293 and COS-7 cells transiently transfected with beta(2)-adrenergic or m2 muscarinic receptors, respectively, treatment with agonist-induced phosphorylation of Akt at serine 473 as evidenced by phosphoserine-specific immunoblots. Serine 174-180 AKT serine/threonine kinase 1 Homo sapiens 167-170 10986289-13 2000 In m1 muscarinic receptor transfected HEK-293 cells, carbachol inhibited insulin-like growth factor-1 stimulated phosphorylation at Ser(473) of endogenous Akt in an atropine-reversible fashion. Serine 132-135 AKT serine/threonine kinase 1 Homo sapiens 155-158 11007772-5 2000 Transduction of cardiomyocytes with constitutively active PI 3-kinase specifically lead to serine phosphorylation of Akt, whereas phosphorylation of IGF-I receptor, IRS1/2 and p44/42 mitogen-activated protein kinase were not increased. Serine 91-97 AKT serine/threonine kinase 1 Homo sapiens 117-120 11035810-2 2000 These serine residues of GSK-3 have been previously identified as targets of protein kinase B (PKB/Akt), a serine/threonine kinase located downstream of phosphatidylinositol 3-kinase. Serine 6-12 AKT serine/threonine kinase 1 Homo sapiens 95-102 11054802-3 2000 We demonstrate that PI3K and Akt (Ser-473 phosphorylation) are activated in response to platelet-derived growth factor but not basic fibroblast growth factor-2 (FGF2) and that distinct forms of PI3K are activated in early progenitors and later-maturation pro-oligodendroblasts as identified by their sensitivity to wortmannin. Serine 34-37 AKT serine/threonine kinase 1 Homo sapiens 29-32 11085518-9 2000 Cisplatin did not stimulate the phosphorylation of BAD Ser-136, but did stimulate the phosphorylation of BAD Ser-112 in cells expressing a dominant-negative Akt, suggesting that BAD Ser-136 but not Ser-112 was phosphorylated by Akt. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 157-160 11085518-9 2000 Cisplatin did not stimulate the phosphorylation of BAD Ser-136, but did stimulate the phosphorylation of BAD Ser-112 in cells expressing a dominant-negative Akt, suggesting that BAD Ser-136 but not Ser-112 was phosphorylated by Akt. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 157-160 11085518-9 2000 Cisplatin did not stimulate the phosphorylation of BAD Ser-136, but did stimulate the phosphorylation of BAD Ser-112 in cells expressing a dominant-negative Akt, suggesting that BAD Ser-136 but not Ser-112 was phosphorylated by Akt. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 157-160 11029403-6 2000 E(2) stimulated rapid Akt phosphorylation on serine 473. Serine 45-51 AKT serine/threonine kinase 1 Homo sapiens 22-25 11007949-9 2000 ILK has been shown to phosphorylate PKB/Akt on Ser-473 in vitro and in vivo. Serine 47-50 AKT serine/threonine kinase 1 Homo sapiens 36-43 11007949-13 2000 Loss of expression or mutational inactivation of PTEN leads to the constitutive activation of PKB/Akt via enhanced phosphorylation of Thr-308 and Ser-473. Serine 146-149 AKT serine/threonine kinase 1 Homo sapiens 94-101 11007949-15 2000 A small molecule ILK inhibitor suppresses the phosphorylation of PKB at the Ser-473 but not the Thr-308 site in the PTEN mutant cells. Serine 76-79 AKT serine/threonine kinase 1 Homo sapiens 65-68 11027222-5 2000 In C6 glioma cells and primary cultures of oligodendrocytes, heregulin induced time- and dose-dependent Akt phosphorylation at Ser(473) in a wortmannin-sensitive manner. Serine 127-130 AKT serine/threonine kinase 1 Homo sapiens 104-107 11029403-7 2000 As has been shown for vascular endothelial growth factor, eNOS is an E(2)-activated Akt substrate, demonstrated by rapid eNOS phosphorylation on serine 1177, a critical residue for eNOS activation and enhanced sensitivity to resting cellular Ca(2+) levels. Serine 145-151 AKT serine/threonine kinase 1 Homo sapiens 84-87 10908564-4 2000 Upon EGF simulation, phosphorylation of Akt1 at the Ser-473 residue was remarkably induced. Serine 52-55 AKT serine/threonine kinase 1 Homo sapiens 40-44 10874027-3 2000 Here we report that the major PKB subtype in platelets is PKBalpha, which is activated by phosphorylation of Thr(308) and Ser(473) and has a constitutively phosphorylated Thr(450) that does not contribute to PKB activation. Serine 122-125 AKT serine/threonine kinase 1 Homo sapiens 58-66 10874027-8 2000 These data reveal a PI-3K-independent PKB activation in which PKCalpha/beta regulates the phosphorylation of Ser(473) in PKBalpha. Serine 109-112 AKT serine/threonine kinase 1 Homo sapiens 121-129 11043856-10 2000 Furthermore, insulin-stimulated translocation of protein kinase B/Akt to the plasma membrane, where serine phosphorylation and full activation occurs, is impaired in Type II diabetes. Serine 100-106 AKT serine/threonine kinase 1 Homo sapiens 66-69 10949937-4 2000 Integrin-linked kinase (ILK) is an integrin-interacting protein kinase which has been identified as a potential PDK-2, as it is capable of phosphorylating PKB/Akt on Ser-473, and stimulating its activity. Serine 166-169 AKT serine/threonine kinase 1 Homo sapiens 155-158 10958690-4 2000 In addition to constitutive membrane association of Itk, unstimulated JTAg T cells also exhibited constitutive phosphorylation of Akt on Ser-473, an indication of elevated basal levels of the phosphatidylinositol 3-kinase (PI3K) products PI-3,4-P(2) and PI-3,4,5-P(3) in the plasma membrane. Serine 137-140 AKT serine/threonine kinase 1 Homo sapiens 130-133 10958690-6 2000 Inhibition of D3 phosphorylation by PI3K inhibitors, or by expression of PTEN, blocked constitutive phosphorylation of Akt on Ser-473 and caused Itk to redistribute to the cytosol. Serine 126-129 AKT serine/threonine kinase 1 Homo sapiens 119-122 10869359-5 2000 The alteration of the serine residues within the Akt consensus sites to alanines results in a progressive increase in enzymatic activity in vitro and in vivo. Serine 22-28 AKT serine/threonine kinase 1 Homo sapiens 49-52 10994851-4 2000 Ischemic preconditioning does not affect the amount of Akt protein, but rather decreases the phosphorylation of Akt at Ser-473 after 10 minutes reperfusion after 3.5 minutes BCAO. Serine 119-122 AKT serine/threonine kinase 1 Homo sapiens 112-115 10949937-4 2000 Integrin-linked kinase (ILK) is an integrin-interacting protein kinase which has been identified as a potential PDK-2, as it is capable of phosphorylating PKB/Akt on Ser-473, and stimulating its activity. Serine 166-169 AKT serine/threonine kinase 1 Homo sapiens 159-162 10908731-2 2000 The protein kinase Akt activates the endothelial NO synthase (eNOS) by phosphorylation of Ser-1177. Serine 90-93 AKT serine/threonine kinase 1 Homo sapiens 19-22 10908731-7 2000 Our data indicate that eNOS activation via phosphorylation of Ser-1177 by Akt is necessary and sufficient for VEGF-mediated EC migration. Serine 62-65 AKT serine/threonine kinase 1 Homo sapiens 74-77 10822383-5 2000 Immunostaining and Western blot analyses using a phospho-ser-473 Akt antibody that detects the activated form of AKT2 (AKT2 phosphorylated at serine-474) confirmed the frequent activation of AKT2 in ovarian cancer specimens. Serine 57-60 AKT serine/threonine kinase 1 Homo sapiens 65-68 10825497-4 2000 Interestingly, lower concentrations of this agent (< or =10 microM) were sufficient to suppress Akt phosphorylation at Ser-473, a putative downstream target of PI 3-kinase, under these conditions. Serine 122-125 AKT serine/threonine kinase 1 Homo sapiens 99-102 10788440-0 2000 Ceramide inhibits protein kinase B/Akt by promoting dephosphorylation of serine 473. Serine 73-79 AKT serine/threonine kinase 1 Homo sapiens 35-38 10880354-3 2000 Ser(136) is thought to be phosphorylated by protein kinase B (PKB, also called Akt), which is activated when cells are exposed to agonists that stimulate phosphatidylinositol 3-kinase (PI3K). Serine 0-3 AKT serine/threonine kinase 1 Homo sapiens 62-65 10880354-3 2000 Ser(136) is thought to be phosphorylated by protein kinase B (PKB, also called Akt), which is activated when cells are exposed to agonists that stimulate phosphatidylinositol 3-kinase (PI3K). Serine 0-3 AKT serine/threonine kinase 1 Homo sapiens 79-82 10893341-7 2000 Insulin-stimulated phosphatidylinositol (PI) 3-kinase, Akt (protein kinase B) serine phosphorylation, and Akt activity were reduced 34, 65, and 20%, respectively, after ECC (P < 0.05). Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 55-58 10898514-6 2000 The down-regulation of cyclin D3 was at the level of translation, and this event was preceded by a pronounced inhibition of Akt/protein kinase B phosphorylation at Ser 473. Serine 164-167 AKT serine/threonine kinase 1 Homo sapiens 124-127 10822383-5 2000 Immunostaining and Western blot analyses using a phospho-ser-473 Akt antibody that detects the activated form of AKT2 (AKT2 phosphorylated at serine-474) confirmed the frequent activation of AKT2 in ovarian cancer specimens. Serine 142-148 AKT serine/threonine kinase 1 Homo sapiens 65-68 10617634-0 2000 Akt protein kinase inhibits Rac1-GTP binding through phosphorylation at serine 71 of Rac1. Serine 72-78 AKT serine/threonine kinase 1 Homo sapiens 0-3 10749665-6 2000 The functionality of the cannabinoid-induced stimulation of PKB was proved by the increased phosphorylation of glycogen synthase kinase-3 serine 21 observed in cannabinoid-treated cells and its prevention by SR141716 and wortmannin. Serine 138-144 AKT serine/threonine kinase 1 Homo sapiens 60-63 10716737-2 2000 Somatic mutations of PTEN are found in a number of human malignancies, and loss of expression, or mutational inactivation of PTEN, leads to the constitutive activation of protein kinase B (PKB)/Akt via enhanced phosphorylation of Thr-308 and Ser-473. Serine 242-245 AKT serine/threonine kinase 1 Homo sapiens 189-192 10716737-2 2000 Somatic mutations of PTEN are found in a number of human malignancies, and loss of expression, or mutational inactivation of PTEN, leads to the constitutive activation of protein kinase B (PKB)/Akt via enhanced phosphorylation of Thr-308 and Ser-473. Serine 242-245 AKT serine/threonine kinase 1 Homo sapiens 194-197 10716737-3 2000 We recently have demonstrated that the integrin-linked kinase (ILK) can phosphorylate PKB/Akt on Ser-473 in a phosphoinositide phospholipid-dependent manner. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 86-89 10716737-3 2000 We recently have demonstrated that the integrin-linked kinase (ILK) can phosphorylate PKB/Akt on Ser-473 in a phosphoinositide phospholipid-dependent manner. Serine 97-100 AKT serine/threonine kinase 1 Homo sapiens 90-93 10716737-5 2000 Transfection of a kinase-deficient, dominant-negative form of ILK or exposure to a small molecule ILK inhibitor suppresses the constitutive phosphorylation of PKB/Akt on Ser-473, but not on Thr-308, in the PTEN-mutant prostate carcinoma cell lines PC-3 and LNCaP. Serine 170-173 AKT serine/threonine kinase 1 Homo sapiens 159-162 10716737-5 2000 Transfection of a kinase-deficient, dominant-negative form of ILK or exposure to a small molecule ILK inhibitor suppresses the constitutive phosphorylation of PKB/Akt on Ser-473, but not on Thr-308, in the PTEN-mutant prostate carcinoma cell lines PC-3 and LNCaP. Serine 170-173 AKT serine/threonine kinase 1 Homo sapiens 163-166 10722653-1 2000 The function of Akt (protein kinase B) is regulated by phosphorylation on two sites conserved within the AGC kinase family: the activation loop (Thr-308) in the kinase core and a hydrophobic phosphorylation site on the carboxyl terminus (Ser-473). Serine 238-241 AKT serine/threonine kinase 1 Homo sapiens 16-19 10722653-4 2000 First we show that a kinase-inactive construct of Akt fails to incorporate phosphate at Ser-473 following IGF-1 stimulation in vivo but does incorporate phosphate at Thr-308 and a second carboxyl-terminal site, Thr-450; this ligand triggers the phosphorylation of both sites in wild-type enzyme. Serine 88-91 AKT serine/threonine kinase 1 Homo sapiens 50-53 10722653-7 2000 Thus, Akt is regulated by autophosphorylation at the Ser-473 hydrophobic site. Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 6-9 10617634-7 2000 Thus, the results suggest that Akt kinase of the phosphoinositide 3-kinase signal transduction pathway phosphorylates serine 71 of Rac1 as one of its authentic substrates and modulates the Rac1 signal transduction pathway through phosphorylation. Serine 118-124 AKT serine/threonine kinase 1 Homo sapiens 31-34 10601235-5 1999 Both p70(S6K) Ser(411) and Akt Ser(473) phosphorylation by Ang II appear to involve EGF receptor transactivation and were inhibited by dominant-negative Ras, whereas the phosphorylation of p70(S6K) and ERK but not Akt was sensitive to the MEK inhibitor. Serine 31-34 AKT serine/threonine kinase 1 Homo sapiens 27-30 10691783-4 2000 Inhibition of phosphatidylinositol 3-kinase (PI3K), using wortmannin or a dominant negative mutant of its downstream target, Akt (protein kinase B), prevented the maintained serine phosphorylation and activation of eNOS. Serine 174-180 AKT serine/threonine kinase 1 Homo sapiens 125-128 10358075-2 1999 Here we show that FKHR, a human homologue of the DAF16 transcription factor in Caenorhabditis elegans, is rapidly phosphorylated by human protein kinase Balpha (PKBalpha) at Thr-24, Ser-256, and Ser-319 in vitro and at a much faster rate than BAD, which is thought to be a physiological substrate for PKB. Serine 182-185 AKT serine/threonine kinase 1 Homo sapiens 138-159 10576742-2 1999 Akt (protein kinase B), a member of a different signaling pathway that also regulates these responses, interacted with Raf and phosphorylated this protein at a highly conserved serine residue in its regulatory domain in vivo. Serine 177-183 AKT serine/threonine kinase 1 Homo sapiens 0-3 10597268-4 1999 It has been shown that phosphorylation of BAD at Ser-136 is mediated by the serine/threonine protein kinase Akt-1/PKB which is downstream of phosphatidylinositol 3-kinase (PI3K). Serine 49-52 AKT serine/threonine kinase 1 Homo sapiens 108-113 10597268-4 1999 It has been shown that phosphorylation of BAD at Ser-136 is mediated by the serine/threonine protein kinase Akt-1/PKB which is downstream of phosphatidylinositol 3-kinase (PI3K). Serine 49-52 AKT serine/threonine kinase 1 Homo sapiens 114-117 10597268-7 1999 While Ser-136 phosphorylation is concordant with activation of Akt, Ser-112 phosphorylation does not correlate with Akt activation. Serine 6-9 AKT serine/threonine kinase 1 Homo sapiens 63-66 10597268-10 1999 Therefore, the PI3K-Akt and Ras-MAPK pathways converge at BAD by mediating phosphorylation of distinct serine residues. Serine 103-109 AKT serine/threonine kinase 1 Homo sapiens 20-23 10491755-8 1999 Although tyrosine phosphorylation patterns were not affected by lipoic acid pretreatment, insulin-stimulated protein kinase B/Akt serine 473 phosphorylation and activity were considerably impaired by oxidation but protected by lipoic acid pretreatment. Serine 130-136 AKT serine/threonine kinase 1 Homo sapiens 126-129 10377430-3 1999 Protein kinase B (PKB)/Akt, a key mediator of PI3 kinase signal transduction, phosphorylated recombinant FKHR1 in vitro at threonine-24 and serine-253. Serine 140-146 AKT serine/threonine kinase 1 Homo sapiens 23-26 10358075-2 1999 Here we show that FKHR, a human homologue of the DAF16 transcription factor in Caenorhabditis elegans, is rapidly phosphorylated by human protein kinase Balpha (PKBalpha) at Thr-24, Ser-256, and Ser-319 in vitro and at a much faster rate than BAD, which is thought to be a physiological substrate for PKB. Serine 195-198 AKT serine/threonine kinase 1 Homo sapiens 138-159 10358075-2 1999 Here we show that FKHR, a human homologue of the DAF16 transcription factor in Caenorhabditis elegans, is rapidly phosphorylated by human protein kinase Balpha (PKBalpha) at Thr-24, Ser-256, and Ser-319 in vitro and at a much faster rate than BAD, which is thought to be a physiological substrate for PKB. Serine 195-198 AKT serine/threonine kinase 1 Homo sapiens 161-169 10358075-2 1999 Here we show that FKHR, a human homologue of the DAF16 transcription factor in Caenorhabditis elegans, is rapidly phosphorylated by human protein kinase Balpha (PKBalpha) at Thr-24, Ser-256, and Ser-319 in vitro and at a much faster rate than BAD, which is thought to be a physiological substrate for PKB. Serine 195-198 AKT serine/threonine kinase 1 Homo sapiens 161-164 10358075-3 1999 The same three sites, which all lie in the canonical PKB consensus sequences (Arg-Xaa-Arg-Xaa-Xaa-(Ser/Thr)), became phosphorylated when FKHR was cotransfected with either PKB or PDK1 (an upstream activator of PKB). Serine 99-102 AKT serine/threonine kinase 1 Homo sapiens 53-56 10358075-3 1999 The same three sites, which all lie in the canonical PKB consensus sequences (Arg-Xaa-Arg-Xaa-Xaa-(Ser/Thr)), became phosphorylated when FKHR was cotransfected with either PKB or PDK1 (an upstream activator of PKB). Serine 99-102 AKT serine/threonine kinase 1 Homo sapiens 172-175 10415336-3 1999 PTEN, when ectopically expressed in human breast carcinoma cells, exhibited an inhibition of phosphorylation of both activating residues of protein kinase B (PKB)/AKT at Ser-473 and Thr-308 without any significant alteration of AKT expression. Serine 170-173 AKT serine/threonine kinase 1 Homo sapiens 158-161 10415336-3 1999 PTEN, when ectopically expressed in human breast carcinoma cells, exhibited an inhibition of phosphorylation of both activating residues of protein kinase B (PKB)/AKT at Ser-473 and Thr-308 without any significant alteration of AKT expression. Serine 170-173 AKT serine/threonine kinase 1 Homo sapiens 163-166 10358075-3 1999 The same three sites, which all lie in the canonical PKB consensus sequences (Arg-Xaa-Arg-Xaa-Xaa-(Ser/Thr)), became phosphorylated when FKHR was cotransfected with either PKB or PDK1 (an upstream activator of PKB). Serine 99-102 AKT serine/threonine kinase 1 Homo sapiens 172-175 10358075-2 1999 Here we show that FKHR, a human homologue of the DAF16 transcription factor in Caenorhabditis elegans, is rapidly phosphorylated by human protein kinase Balpha (PKBalpha) at Thr-24, Ser-256, and Ser-319 in vitro and at a much faster rate than BAD, which is thought to be a physiological substrate for PKB. Serine 182-185 AKT serine/threonine kinase 1 Homo sapiens 161-169 10358075-8 1999 The conservation of Thr-24, Ser-256, and Ser-319 and the sequences surrounding them in DAF16 therefore suggests that DAF16 is also a direct substrate for PKB in C. elegans. Serine 28-31 AKT serine/threonine kinase 1 Homo sapiens 154-157 10358075-8 1999 The conservation of Thr-24, Ser-256, and Ser-319 and the sequences surrounding them in DAF16 therefore suggests that DAF16 is also a direct substrate for PKB in C. elegans. Serine 41-44 AKT serine/threonine kinase 1 Homo sapiens 154-157 10358075-2 1999 Here we show that FKHR, a human homologue of the DAF16 transcription factor in Caenorhabditis elegans, is rapidly phosphorylated by human protein kinase Balpha (PKBalpha) at Thr-24, Ser-256, and Ser-319 in vitro and at a much faster rate than BAD, which is thought to be a physiological substrate for PKB. Serine 182-185 AKT serine/threonine kinase 1 Homo sapiens 161-164 10187851-2 1999 Hydrogen peroxide (1 mM) markedly induced tyrosine phosphorylation of focal adhesion kinase (FAK) and serine phosphorylation of Akt at 1 h after stimulation. Serine 102-108 AKT serine/threonine kinase 1 Homo sapiens 128-131 10347145-9 1999 Mutation of serine 253, located in a consensus Akt phosphorylation site at the carboxyl-terminal end of the forkhead domain, abolished the effect of insulin on FKHR phosphorylation. Serine 12-18 AKT serine/threonine kinase 1 Homo sapiens 47-50 10347145-10 1999 In contrast, mutation of two additional Akt phosphorylation sites, at amino acids threonine 24 or serine 316, did not abolish insulin-induced phosphorylation. Serine 98-104 AKT serine/threonine kinase 1 Homo sapiens 40-43 10362672-2 1999 Stimulation of the cells with ANG II led to a marked increase in the kinase activity of Akt/PKB, which coincided with Ser-473 phosphorylation. Serine 118-121 AKT serine/threonine kinase 1 Homo sapiens 88-95 10376602-3 1999 Here we show that the serine/threonine protein kinase Akt (protein kinase B) can directly phosphorylate eNOS on serine 1179 and activate the enzyme, leading to NO production, whereas mutant eNOS (S1179A) is resistant to phosphorylation and activation by Akt. Serine 22-28 AKT serine/threonine kinase 1 Homo sapiens 54-57 10376602-3 1999 Here we show that the serine/threonine protein kinase Akt (protein kinase B) can directly phosphorylate eNOS on serine 1179 and activate the enzyme, leading to NO production, whereas mutant eNOS (S1179A) is resistant to phosphorylation and activation by Akt. Serine 22-28 AKT serine/threonine kinase 1 Homo sapiens 254-257 10376603-5 1999 Inhibition of the phosphatidylinositol-3-OH kinase/Akt pathway or mutation of the Akt site on eNOS protein (at serine 1177) attenuates the serine phosphorylation and prevents the activation of eNOS. Serine 111-117 AKT serine/threonine kinase 1 Homo sapiens 82-85 10376603-5 1999 Inhibition of the phosphatidylinositol-3-OH kinase/Akt pathway or mutation of the Akt site on eNOS protein (at serine 1177) attenuates the serine phosphorylation and prevents the activation of eNOS. Serine 139-145 AKT serine/threonine kinase 1 Homo sapiens 51-54 10376603-5 1999 Inhibition of the phosphatidylinositol-3-OH kinase/Akt pathway or mutation of the Akt site on eNOS protein (at serine 1177) attenuates the serine phosphorylation and prevents the activation of eNOS. Serine 139-145 AKT serine/threonine kinase 1 Homo sapiens 82-85 9722592-5 1998 Activation of Akt by DAMGO correlates with its phosphorylation at serine 473. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 14-17 9859994-3 1998 Here we report that Ca2+/calmodulin-dependent protein kinase kinase (CaM-KK) activates PKB directly, resulting in phosphorylation of BAD on serine residue 136 and the interaction of BAD with protein 14-3-3. Serine 140-146 AKT serine/threonine kinase 1 Homo sapiens 87-90 9736715-8 1998 In addition, kinase-active ILK can phosphorylate PKB/AKT on serine-473, whereas kinase-deficient ILK severely inhibits endogenous phosphorylation of PKB/AKT on serine-473, demonstrating that ILK is involved in agonist stimulated, Pi(3)K-dependent, PKB/AKT activation. Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 49-52 9736715-8 1998 In addition, kinase-active ILK can phosphorylate PKB/AKT on serine-473, whereas kinase-deficient ILK severely inhibits endogenous phosphorylation of PKB/AKT on serine-473, demonstrating that ILK is involved in agonist stimulated, Pi(3)K-dependent, PKB/AKT activation. Serine 60-66 AKT serine/threonine kinase 1 Homo sapiens 53-56 9736715-8 1998 In addition, kinase-active ILK can phosphorylate PKB/AKT on serine-473, whereas kinase-deficient ILK severely inhibits endogenous phosphorylation of PKB/AKT on serine-473, demonstrating that ILK is involved in agonist stimulated, Pi(3)K-dependent, PKB/AKT activation. Serine 160-166 AKT serine/threonine kinase 1 Homo sapiens 149-152 9736715-8 1998 In addition, kinase-active ILK can phosphorylate PKB/AKT on serine-473, whereas kinase-deficient ILK severely inhibits endogenous phosphorylation of PKB/AKT on serine-473, demonstrating that ILK is involved in agonist stimulated, Pi(3)K-dependent, PKB/AKT activation. Serine 160-166 AKT serine/threonine kinase 1 Homo sapiens 153-156 9736715-8 1998 In addition, kinase-active ILK can phosphorylate PKB/AKT on serine-473, whereas kinase-deficient ILK severely inhibits endogenous phosphorylation of PKB/AKT on serine-473, demonstrating that ILK is involved in agonist stimulated, Pi(3)K-dependent, PKB/AKT activation. Serine 160-166 AKT serine/threonine kinase 1 Homo sapiens 149-152 9736715-8 1998 In addition, kinase-active ILK can phosphorylate PKB/AKT on serine-473, whereas kinase-deficient ILK severely inhibits endogenous phosphorylation of PKB/AKT on serine-473, demonstrating that ILK is involved in agonist stimulated, Pi(3)K-dependent, PKB/AKT activation. Serine 160-166 AKT serine/threonine kinase 1 Homo sapiens 153-156 9829964-4 1998 When overexpressed in serum-stimulated cells, Akt/PKB potently induced Ser-133 phosphorylation of CREB and promoted recruitment of CBP. Serine 71-74 AKT serine/threonine kinase 1 Homo sapiens 50-53 9829964-5 1998 Correspondingly, Akt/PKB stimulated target gene expression via CREB in a phospho(Ser-133)-dependent manner. Serine 81-84 AKT serine/threonine kinase 1 Homo sapiens 17-24 9812896-4 1998 Akt phosphorylated recombinant Casp9 in vitro on serine-196 and inhibited its protease activity. Serine 49-55 AKT serine/threonine kinase 1 Homo sapiens 0-3 9786927-5 1998 We report here the identification of a novel serine- and proline-rich GTPase-activating protein, CdGAP, which is active in vitro on both Cdc42 and Rac. Serine 45-51 AKT serine/threonine kinase 1 Homo sapiens 147-150 9660823-7 1998 Further, Ser/Thr phosphorylation of downstream molecules Akt and p70 S6 kinase was inhibited. Serine 9-12 AKT serine/threonine kinase 1 Homo sapiens 57-60 9373175-0 1997 Further evidence that the inhibition of glycogen synthase kinase-3beta by IGF-1 is mediated by PDK1/PKB-induced phosphorylation of Ser-9 and not by dephosphorylation of Tyr-216. Serine 131-134 AKT serine/threonine kinase 1 Homo sapiens 100-103 9374542-5 1997 Activation was due to phosphorylation on Thr-309 and Ser-474, which correspond to Thr-308 and Ser-473 implicated in the regulation of PKBalpha. Serine 53-56 AKT serine/threonine kinase 1 Homo sapiens 134-142 9374542-5 1997 Activation was due to phosphorylation on Thr-309 and Ser-474, which correspond to Thr-308 and Ser-473 implicated in the regulation of PKBalpha. Serine 94-97 AKT serine/threonine kinase 1 Homo sapiens 134-142 9373175-5 1997 Coexpression of WT-GSK3beta in 293 cells with either PKB alpha (also known as AKT) or PDK1 (the "upstream" activator of PKB) mimicked the IGF-1- or insulin-induced phosphorylation of Ser-9 and inactivation of GSK3beta. Serine 183-186 AKT serine/threonine kinase 1 Homo sapiens 78-81 8985174-4 1996 PKBalpha phosphorylated histone H2B (Km 5 microM, Vmax 68 U/mg) specifically at Ser-36 which also lies in an Arg-Xaa-Arg-Xaa-Xaa-Ser-Hyd motif. Serine 80-83 AKT serine/threonine kinase 1 Homo sapiens 0-8 8985174-4 1996 PKBalpha phosphorylated histone H2B (Km 5 microM, Vmax 68 U/mg) specifically at Ser-36 which also lies in an Arg-Xaa-Arg-Xaa-Xaa-Ser-Hyd motif. Serine 129-132 AKT serine/threonine kinase 1 Homo sapiens 0-8 1833819-4 1991 This suggests that Akt may form a functional link between tyrosine and serine-threonine phosphorylation pathways. Serine 71-77 AKT serine/threonine kinase 1 Homo sapiens 19-22 8650155-7 1996 Deletion of the serine/threonine rich C-terminal region impaired both RAC-PKalpha basal and vanadate-stimulated activity. Serine 16-22 AKT serine/threonine kinase 1 Homo sapiens 70-81 33769916-4 2021 The tyrosine kinase A (TrkA) and phosphoinositide 3-kinase (PI3K)-serine/threonine protein kinase (Akt) signaling pathways in hUCMSCs were involved in the NGF-induced protection. Serine 66-72 AKT serine/threonine kinase 1 Homo sapiens 99-102 33796531-1 2021 The p21-activated kinases (PAKs), downstream effectors of Ras-related Rho GTPase Cdc42 and Rac, are serine/threonine kinases. Serine 100-106 AKT serine/threonine kinase 1 Homo sapiens 91-94 33941854-7 2021 In particular, SNORD12B could interact with PP-1alpha, one of the three catalytic subunits of serine/threonine protein phosphatase 1, which is a major phosphatase that directly dephosphorylates AKT to suppress its activation. Serine 94-100 AKT serine/threonine kinase 1 Homo sapiens 194-197 33815291-5 2021 Adverse effects of sphingolipid accumulation have recently been linked to the activation of protein kinase Czeta (PKCzeta) and protein phosphatase 2A (PP2A), which, in turn, negatively affect phosphorylation of serine/threonine kinase Akt [also known as protein kinase B (PKB)], leading to decreased glucose uptake in skeletal muscles as well as increased gluconeogenesis and glycogenolysis in the liver. Serine 211-217 AKT serine/threonine kinase 1 Homo sapiens 235-238 33815291-5 2021 Adverse effects of sphingolipid accumulation have recently been linked to the activation of protein kinase Czeta (PKCzeta) and protein phosphatase 2A (PP2A), which, in turn, negatively affect phosphorylation of serine/threonine kinase Akt [also known as protein kinase B (PKB)], leading to decreased glucose uptake in skeletal muscles as well as increased gluconeogenesis and glycogenolysis in the liver. Serine 211-217 AKT serine/threonine kinase 1 Homo sapiens 272-275 33802884-6 2021 Western bolt assay showed that Serine/threonine Kinase (Akt) signaling related proteins including Ataxia telangiectasia mutated kinase (ATM), Phosphatase and tensin homolog (PTEN), Akt, Mammalian target of rapamycin (mTOR), Ribosome S6 protein kinase (p70S6K) and e IF4E-binding protein 1(4E-BP1) were regulated, and Hypoxia inducible factor-1alpha (HIF-1alpha) protein expression was decreased by TSE1 in OVCAR-3 cells. Serine 31-37 AKT serine/threonine kinase 1 Homo sapiens 56-59 33802884-6 2021 Western bolt assay showed that Serine/threonine Kinase (Akt) signaling related proteins including Ataxia telangiectasia mutated kinase (ATM), Phosphatase and tensin homolog (PTEN), Akt, Mammalian target of rapamycin (mTOR), Ribosome S6 protein kinase (p70S6K) and e IF4E-binding protein 1(4E-BP1) were regulated, and Hypoxia inducible factor-1alpha (HIF-1alpha) protein expression was decreased by TSE1 in OVCAR-3 cells. Serine 31-37 AKT serine/threonine kinase 1 Homo sapiens 181-184 34973450-9 2022 Mechanistically, lactate increases the activity of adenosine monophosphate-activated protein kinase (AMPK) and suppresses the phosphorylation of serine/threonine kinase 1 (Akt) and mammalian target of rapamycin (mTOR). Serine 145-151 AKT serine/threonine kinase 1 Homo sapiens 172-175 34724321-9 2022 We found that DAS disrupted the positive regulatory loop between ER-alpha36 and EGFR, and decreased the phosphorylation of AKT at Ser 473 both in vivo and in vitro. Serine 130-133 AKT serine/threonine kinase 1 Homo sapiens 123-126 34974186-5 2022 Various key findings in podocyte autophagy were reported in the past ten years, such as the role of endoplasmic reticulum (ER) stress in podocyte autophagy impairment, podocyte autophagy-related gene, essential roles of the signaling pathways: Mammalian Target of Rapamycin (mTOR)/ Phosphoinositide 3-kinase (PI3k)/ serine/threonine kinase 1 (Akt) in podocyte autophagy. Serine 316-322 AKT serine/threonine kinase 1 Homo sapiens 343-346 34697424-1 2022 The p21-Activated Kinases (PAKs) are a family of six serine/threonine kinases that were originally identified as downstream effectors of the Rho GTPases Cdc42 and Rac. Serine 53-59 AKT serine/threonine kinase 1 Homo sapiens 163-166 34318428-6 2022 The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelial-mesenchymal transition (EMT) was detected by Western blot assays. Serine 41-47 AKT serine/threonine kinase 1 Homo sapiens 37-40 34963894-1 2021 The serine/threonine protein kinase Akt regulates a wide range of cellular functions via phosphorylation of various substrates distributed throughout the cell, including at the plasma membrane and endomembrane compartments. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 36-39 34839095-3 2022 Akt is a serine-threonine protein kinase with main phosphorylation sites at Thr308 and Ser473, which are critical to generate a high level of Akt activity. Serine 9-15 AKT serine/threonine kinase 1 Homo sapiens 0-3 34839095-3 2022 Akt is a serine-threonine protein kinase with main phosphorylation sites at Thr308 and Ser473, which are critical to generate a high level of Akt activity. Serine 9-15 AKT serine/threonine kinase 1 Homo sapiens 142-145 34965411-5 2021 In parallel, downregulation of SIRT2 hyperacetylates the serine/threonine protein kinase AKT1 at Lys20 in a non-canonical way, activating DRP1 and metabolic reprogramming. Serine 57-63 AKT serine/threonine kinase 1 Homo sapiens 89-93 34757844-2 2022 Here, we revealed that SVV infection inhibited the phosphorylation of mechanistic target of rapamycin kinase (MTOR) and activated phosphorylation of the serine/threonine kinase AKT. Serine 153-159 AKT serine/threonine kinase 1 Homo sapiens 177-180 34855399-1 2021 The serine/threonine kinase AKT functions as a critical node of the phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of rapamycin (m-TOR) signaling pathway. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 34855399-1 2021 The serine/threonine kinase AKT functions as a critical node of the phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of rapamycin (m-TOR) signaling pathway. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 105-108 34695518-5 2021 We found adriamycin-induced VSMC senescence was accompanied by reduced PTEN protein expression and upregulation of the mTORC2-Akt (Ser 473) pathway and that fisetin treatment reduced VSMC senescence by increasing PTEN and decreasing mTORC2 protein levels. Serine 131-134 AKT serine/threonine kinase 1 Homo sapiens 126-129 34948236-2 2021 Akt is a phosphoinositide-dependent serine/threonine kinase, controlling numerous pathological processes, including oncogenesis and autoimmunity. Serine 36-42 AKT serine/threonine kinase 1 Homo sapiens 0-3 34948236-4 2021 The expression of the entire and activated forms of Akt (partially and fully activated: phosphorylated at threonine-308 (T308) and serine-473 (S473), respectively), and two of its substrates, the proline-rich Akt-substrate of 40 kDa (PRAS40) and FoxO1 transcription factor has been immunohistochemically examined in minor salivary glands (MSG) of pSS patients (n = 29; including 9 with pSS-associated lymphoma) and sicca-complaining controls (sicca-controls; n = 10). Serine 131-137 AKT serine/threonine kinase 1 Homo sapiens 52-55 34948057-5 2021 Our results show that the surface movement of TrkA and p75NTR and the activation of TrkA- and p75NTR-related phosphoinositide-3-kinase (PI3K)/serine/threonine-protein kinase (AKT) signaling pathways are altered in neurons that are derived from patients suffering from fAD compared to controls. Serine 142-148 AKT serine/threonine kinase 1 Homo sapiens 175-178 34891098-7 2021 We uncover the mechanism of heme-mediated platelet mtROS production which is dependent on the activation of platelet toll-like receptor 4 (TLR4) signaling and leads to the downstream phosphorylation and inhibition of complex-V by the serine kinase Akt. Serine 234-240 AKT serine/threonine kinase 1 Homo sapiens 248-251 34695518-6 2021 Furthermore, PTEN played a primary role in the anti-aging effect of fisetin, and fisetin-activated PTEN directly regulated the mTORC2-Akt (Ser 473) signaling pathway, and attenuated senescence phenotypes such as senescence-associated beta-galactosidase (SA-beta-gal) and the p53-p21 signaling pathway in VSMCs. Serine 139-142 AKT serine/threonine kinase 1 Homo sapiens 134-137 34787460-4 2022 In this study, we explored the effect of Akt serine 473 phosphorylation (p-Akt) during murine norovirus (MNV) infection. Serine 45-51 AKT serine/threonine kinase 1 Homo sapiens 41-44 33724154-6 2021 The improved understanding of JE pathogenesis might lay a foundation for the development of novel therapies to halt JE.Abbreviations AKT: a serine/threonine-specific protein kinase; AP1: activator protein 1; ASC: apoptosis-associated speck-like protein containing a CARD; ASK1: apoptosis signal-regulated kinase 1; ATF3/4/6: activating transcription factor 3/4/6; ATG5/7: autophagy-related 5/7; BBB: blood-brain barrier; Bcl-3/6: B-cell lymphoma 3/6 protein; CCL: C-C motif chemokine ligand; CCR2: C-C motif chemokine receptor 2; CHOP: C/EBP homologous protein; circRNA: circular RNA; CNS: central nervous system; CXCL: C-X-C motif chemokine ligand; dsRNA: double-stranded RNA; EDEM1: endoplasmic reticulum degradation enhancer mannosidase alpha-like 1; eIF2-alpha: eukaryotic initiation factor 2 alpha; ER: endoplasmic reticulum; ERK: extracellular signal-regulated kinase; GRP78: 78-kDa glucose-regulated protein; ICAM: intercellular adhesion molecule; IFN: interferon; IL: interleukin; iNOS: inducible nitric oxide synthase; IRAK1/2: interleukin-1 receptor-associated kinase 1/2; IRE-1: inositol-requiring enzyme 1; IRF: interferon regulatory factor; ISG15: interferon-stimulated gene 15; JE: Japanese encephalitis; JEV: Japanese encephalitis virus; JNK: c-Jun N-terminal kinase; LAMP2: lysosome-associated membrane protein type 2; LC3-I/II: microtubule-associated protein 1 light chain 3-I/II; lncRNA: long non-coding RNA; MAPK: mitogen-activated protein kinase; miR/miRNA: microRNA; MK2: mitogen-activated protein kinase-activated protein kinase 2; MKK4: mitogen-activated protein kinase kinase 4; MLKL: mixed-linage kinase domain-like protein; MMP: matrix metalloproteinase; MyD88: myeloid differentiation factor 88; Nedd4: neural precursor cell-expressed developmentally downregulated 4; NF-kappaB: nuclear factor kappa B; NKRF: nuclear factor kappa B repressing factor; NLRP3: NLR family pyrin domain containing 3; NMDAR: N-methyl-D-aspartate receptor; NO: nitric oxide; NS2B/3/4: JEV non-structural protein 2B/3/4; P: phosphorylation. Serine 140-146 AKT serine/threonine kinase 1 Homo sapiens 133-136 34801692-6 2022 Furthermore, both EPA (200 muM) and DHA (100 muM) may significantly promote the serine/threonine protein kinase (Akt) phosphorylation by 70% and 40% in the physiological state of adipocytes, respectively. Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 113-116 34858045-1 2021 The serine/threonine kinase AKT is a critical effector of the phosphoinositide 3-kinase (PI3K) signaling cascade and has a pivotal role in cell growth, proliferation, survival, and metabolism. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 34551282-1 2021 AKT is a serine/threonine kinase that plays an important role in metabolism, cell growth, and cytoskeletal dynamics. Serine 9-15 AKT serine/threonine kinase 1 Homo sapiens 0-3 34787460-4 2022 In this study, we explored the effect of Akt serine 473 phosphorylation (p-Akt) during murine norovirus (MNV) infection. Serine 45-51 AKT serine/threonine kinase 1 Homo sapiens 75-78 34593522-6 2021 Mechanistically, HIF1A-AS1 facilitated the interaction between serine/threonine kinase AKT and Y-box binding protein 1 (YB1), which promoted phosphorylation of YB1 (pYB1). Serine 63-69 AKT serine/threonine kinase 1 Homo sapiens 87-90 34725436-7 2021 Akt suppression following sorafenib treatment resulted in an increase of the H3K27me3 levels through a decrease in EZH2 phosphorylation at serine 21. Serine 139-145 AKT serine/threonine kinase 1 Homo sapiens 0-3 34464661-3 2021 The evolutionarily conserved serine/threonine protein kinase B (PKB, also referred to as Akt) -mammalian target of rapamycin (mTOR) pathway is universal to nearly all multicellular organisms and plays an integral role in most cellular processes. Serine 29-35 AKT serine/threonine kinase 1 Homo sapiens 64-67 34464661-3 2021 The evolutionarily conserved serine/threonine protein kinase B (PKB, also referred to as Akt) -mammalian target of rapamycin (mTOR) pathway is universal to nearly all multicellular organisms and plays an integral role in most cellular processes. Serine 29-35 AKT serine/threonine kinase 1 Homo sapiens 89-92 34499618-7 2021 PKN2-mediated eNOS phosphorylation at serine 1177 involved phosphorylation of AKT synergistically with mTORC2-mediated AKT phosphorylation while active PKN2 directly phosphorylated human eNOS at serine 1179. Serine 38-44 AKT serine/threonine kinase 1 Homo sapiens 78-81 34358366-8 2021 Phosphorylation of Akt Ser 473 was also induced by EGF and TGFalpha and a one-hour pre-treatment with the tyrosine kinas inhibitor(s) reduced this phosphorylation. Serine 23-26 AKT serine/threonine kinase 1 Homo sapiens 19-22 34670536-1 2021 BACKGROUND: The V-Akt murine thymoma viral oncogene (AKT) 1 (E17K) is a subfamily of serine/threonine protein kinases that affects the survival, proliferation, and invasion of cancer cells. Serine 85-91 AKT serine/threonine kinase 1 Homo sapiens 16-59 34769278-9 2021 Furthermore, we found that nivolumab and IL-10 differentially modulate the signal transducer and activator of transcription 3 (STAT3) and AKT serine-threonine kinase pathways. Serine 142-148 AKT serine/threonine kinase 1 Homo sapiens 138-141 34716294-4 2021 As a novel phosphoinositide 3-kinase (PI3K)/mTOR dual inhibitor, BEZ235, exerts antitumour activity by effectively and specifically blocking the dysfunctional activation of the PI3K/serine/threonine kinase (AKT)/mTOR pathway. Serine 182-188 AKT serine/threonine kinase 1 Homo sapiens 207-210 34682865-12 2021 Treatment of LNCaP and PC-3 cells with diosmetin inhibited the phosphorylation of Rictor (Thr-1135), AKT (Ser-473) and PKCalpha (Ser-657) in a dose-dependent manner. Serine 106-109 AKT serine/threonine kinase 1 Homo sapiens 101-104 34768807-1 2021 Hyperactivity of serine-threonine kinase AKT is one of the most common molecular abnormalities in cancer, where it contributes to poor outcomes by facilitating the growth and survival of malignant cells. Serine 17-23 AKT serine/threonine kinase 1 Homo sapiens 41-44 34682865-10 2021 Increased phosphorylation of Rictor at the Thr-1135 residue, AKT at the Ser-473 residue and PKCalpha at the Ser-657 residue were observed after treatment with IGF-1 and IL-6. Serine 72-75 AKT serine/threonine kinase 1 Homo sapiens 61-64 34241580-9 2021 Importantly, we further confirmed that ZBED3-AS1 promoted the malignant progression of melanoma by regulating miR-381-3p/ARID4B axis to activate the phosphatidylinositol 3-kinase/AKT serine/threonine kinase (PI3K/AKT) signaling pathway. Serine 183-189 AKT serine/threonine kinase 1 Homo sapiens 213-216 34645797-7 2021 The TSSK4 kinase activity is found to be closely related to the function of HSP90-AKT pathway that TSSK4 can phosphorylate its substrate HSP90beta on serine 255, to inhibit the ATPase activity of HSP90beta and reduce its molecular chaperone function on AKT. Serine 150-156 AKT serine/threonine kinase 1 Homo sapiens 82-85 33433498-5 2021 Here we showed that both exendin-4 and DA-CH5 protected against 6-hydroxydopamine (6-OHDA) cytotoxicity, inhibited apoptosis, improved mitogenesis and induced autophagy flux in SH-SY5Y cells via activation of the insulin receptor substrate-1 (IRS-1)/alpha serine/threonine-protein kinase (Akt)/cAMP response element-binding protein (CREB) pathway. Serine 256-262 AKT serine/threonine kinase 1 Homo sapiens 289-292 34320369-1 2021 PH domain leucine-rich repeat protein phosphatase (PHLPP) is a family of enzymes made up of two isoforms (PHLPP1 and PHLPP2), whose actions modulate intracellular activity via the dephosphorylation of specific serine/threonine (Ser/Thr) residues on proteins such as Akt. Serine 210-216 AKT serine/threonine kinase 1 Homo sapiens 266-269 34320369-1 2021 PH domain leucine-rich repeat protein phosphatase (PHLPP) is a family of enzymes made up of two isoforms (PHLPP1 and PHLPP2), whose actions modulate intracellular activity via the dephosphorylation of specific serine/threonine (Ser/Thr) residues on proteins such as Akt. Serine 228-231 AKT serine/threonine kinase 1 Homo sapiens 266-269 34603386-4 2021 Akt, a serine/threonine kinase, can modulate the role of several downstream proteins involved in cell proliferation, migration, invasion, metabolism, and apoptosis. Serine 7-13 AKT serine/threonine kinase 1 Homo sapiens 0-3 34405859-6 2021 In addition, FTY720 induced autophagic flux, LC3B-II upregulation, Akt phosphorylation inhibition on Serine 473 but downregulated TFEB, suggesting stalled autophagy. Serine 101-107 AKT serine/threonine kinase 1 Homo sapiens 67-70 34419139-6 2021 The serine/threonine kinase Akt is frequently activated in various types of cancer. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 34452154-1 2021 The phosphatidylinositol 3-kinase (PI3K)/serine-threonine kinase (Akt)/mammalian target of the rapamycin (mTOR)-signaling pathway has been suggested to have connections with the malignant transformation, growth, proliferation, and metastasis of various cancers and solid tumors. Serine 41-47 AKT serine/threonine kinase 1 Homo sapiens 66-69 34093748-6 2021 Mediation of the COPB2 silencing effect may be associated with regulating the phosphorylation of serine/threonine kinase AKT in the PI3K/AKT signaling pathway. Serine 97-103 AKT serine/threonine kinase 1 Homo sapiens 121-124 34093748-6 2021 Mediation of the COPB2 silencing effect may be associated with regulating the phosphorylation of serine/threonine kinase AKT in the PI3K/AKT signaling pathway. Serine 97-103 AKT serine/threonine kinase 1 Homo sapiens 137-140 34359752-8 2021 Together, the results of a previous report demonstrated that AKT mediates PKM2 phosphorylation at serine-202; these results suggest that IGF-1R signaling and PKM2 mutually regulate each other to facilitate cell growth and survival, particularly under hypoxic conditions, in solid tumors with dysregulated IGF-1R expression. Serine 98-104 AKT serine/threonine kinase 1 Homo sapiens 61-64 34294681-6 2021 Furthermore, overexpression of GLT8D2 activated FGFR/PI3K signaling axis and upregulated the phosphorylation levels of FRS2a and AKT (AKT serine/threonine kinase). Serine 138-144 AKT serine/threonine kinase 1 Homo sapiens 129-132 34472272-8 2021 As demonstrated by the Western blot test, astragaloside IV significantly down-regulated the protein expression of serine/threonine-protein kinase(Akt1) and hypoxia-inducible factor 1alpha(HIF-1alpha) in the HIF-1 signaling pathway, and up-regulated the protein expression of vascular endothelial growth factor A(VEGFA). Serine 114-120 AKT serine/threonine kinase 1 Homo sapiens 146-150 34152396-5 2021 Kinome analysis of dexamethasone-treated HMCLs revealed a reduction in serine/threonine peptide phosphorylation, which was predicted to result from reduced Akt activity. Serine 71-77 AKT serine/threonine kinase 1 Homo sapiens 156-159 34230143-3 2021 MATERIALS AND METHODS: We investigated the molecular mechanism of the antitumor effect of metformin alone and in combination with AKT serine/threonine kinase (AKT) inhibition via cell viability and western blot analyses. Serine 134-140 AKT serine/threonine kinase 1 Homo sapiens 159-162 34230143-4 2021 RESULTS: Notably, metformin increased the phosphorylation of AKT at serine 473 using protein array screening. Serine 68-74 AKT serine/threonine kinase 1 Homo sapiens 61-64 34408809-0 2021 Type II transmembrane serine proteases 4 (TMPRSS4) promotes proliferation, invasion and epithelial-mesenchymal transition in endometrial carcinoma cells (HEC1A and Ishikawa) via activation of MAPK and AKT. Serine 22-28 AKT serine/threonine kinase 1 Homo sapiens 201-204 34081908-3 2021 We observe waves of extracellular-signal-regulated kinase (ERK) and AKT serine/threonine kinase (Akt) activity pulses that originate from apoptotic cells and propagate radially to healthy surrounding cells. Serine 72-78 AKT serine/threonine kinase 1 Homo sapiens 97-100 34200497-7 2021 Further, depletion of Yap reduced TGFbeta/hypoxia-induced cardiac fibroblast proliferation and Akt phosphorylation at Ser 473 and Thr308, without any significant effect on TGFbeta/hypoxia-induced ERK1/2 activation or reduction in S6 and S6 kinase activities. Serine 118-121 AKT serine/threonine kinase 1 Homo sapiens 95-98 34073781-6 2021 The BSD-mediated biological response to increase GLUT4 translocation was obliterated by PI-3-K inhibitor wortmannin, and BSD significantly increased the phosphorylation of AKT (Ser-473). Serine 177-180 AKT serine/threonine kinase 1 Homo sapiens 172-175 34206047-6 2021 TCL1A acts as a coactivator of the serine threonine kinase Akt and through other interactions favoring cell survival, growth, and proliferation. Serine 35-41 AKT serine/threonine kinase 1 Homo sapiens 59-62 34938598-8 2021 Further, circPTEN was validated to inhibit K63-linked ubiquitination of protein kinase B (AKT) and AKT phosphorylation at Thr-308 and Ser-473 by competitively binding with tumor necrosis factor (TNF)-receptor-associated factor 6 (TRAF6). Serine 134-137 AKT serine/threonine kinase 1 Homo sapiens 99-102 34066419-4 2021 We discovered that RAB7A regulates AKT and PAK1, and we demonstrated that increased vimentin phosphorylation at Ser38 (Serine 38), observed upon RAB7A overexpression, is due to AKT activity. Serine 119-125 AKT serine/threonine kinase 1 Homo sapiens 35-38 34589270-9 2021 Downregulated FOXO3 was identified in OC, whereas miR-29a-3p targeted FOXO3 to suppress glycogen synthase kinase 3beta (GSK3beta) activity via the serine/threonine protein kinase (AKT)/GSK3beta pathway. Serine 147-153 AKT serine/threonine kinase 1 Homo sapiens 180-183 34065746-1 2021 The serine/threonine kinase AKT is a major effector during phosphatidylinositol 3-kinase (PI3K)-driven cell signal transduction in response to extracellular stimuli. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 34066419-4 2021 We discovered that RAB7A regulates AKT and PAK1, and we demonstrated that increased vimentin phosphorylation at Ser38 (Serine 38), observed upon RAB7A overexpression, is due to AKT activity. Serine 119-125 AKT serine/threonine kinase 1 Homo sapiens 177-180 35176419-3 2022 p70S6K is a critical downstream effector of the oncogenic PI3K/Akt/mTOR pathway and its activation is tightly regulated by an ordered cascade of Ser/Thr phosphorylation events. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 63-66 34064589-5 2021 Therefore, we sought to review the role of the serine/threonine kinase AKT in bone metastasis. Serine 47-53 AKT serine/threonine kinase 1 Homo sapiens 71-74 35438043-10 2022 Our findings suggest a crucial regulatory and adaptive function of FKBP5-regulated autophagy within the MBH in response to metabolic challenges.Abbreviations: AKT: thymoma viral proto-oncogene; AMPK: AMP-activated protein kinase; BECN1: beclin 1, autophagy related; eWAT: epididymal white adipose tissue; FKBP5/FKBP51: FK506 binding protein 5; KO, knockout; MBH, mediobasal hypothalamus; MTORC1, mechanistic target of rapamycin kinase complex 1; p: phosphorylated; PHLPP: PH domain and leucine rich repeat protein phosphatase; RPS6KB/p70S6K: ribosomal protein S6 kinase; SKP2: S-phase kinase-associated protein 2; SM: soleus muscle; SQSTM1/p62, sequestosome 1; STK11/LKB1: serine/threonine kinase 11; TSC: TSC complex; ULK1: unc-51 like kinase 1; WIPI: WD repeat domain, phosphoinositide interacting; WT: wild type. Serine 673-679 AKT serine/threonine kinase 1 Homo sapiens 159-162 35607451-14 2022 Interestingly, melatonin induced the phosphorylation of protein kinase B/AKT at serine 473 residue and GSK-3beta at serine 9 residue. Serine 80-86 AKT serine/threonine kinase 1 Homo sapiens 73-76 35561312-6 2022 Signal transduction was analysed by measuring consecutive phosphorylation of serine/threonine-protein kinase Akt. Serine 77-83 AKT serine/threonine kinase 1 Homo sapiens 109-112 35547877-6 2022 Moreover, the internalization of exosomes from EveR into parental cells induced deregulation of the mTOR pathway mainly by triggering the activation of the serine/threonine protein kinase Akt, involved in the cellular survival pathway, as demonstrated by Western blot analysis. Serine 156-162 AKT serine/threonine kinase 1 Homo sapiens 188-191 35124166-4 2022 Here, we showed protein kinase B/Akt1, a serine/ threonine kinase implicated in schizophrenia, phosphorylated dysbindin-1A at serine 10 (S10). Serine 41-47 AKT serine/threonine kinase 1 Homo sapiens 33-37 35201653-5 2022 The effects of miR-186-5p and ITGA6 on the cell migration, proliferation, and phosphatidylinositol 3-kinase (PI3K)/serine-threonine kinase (AKT) pathway activity were evaluated by transwell assay, cell counting kit 8 assay, and western blotting, respectively. Serine 115-121 AKT serine/threonine kinase 1 Homo sapiens 140-143 35277472-2 2022 Here, we show AKT serine/threonine kinase-dependent epigenetic control of ACE2 and TMPRSS2 expression by high-cannabidiol (CBD) cannabis extracts and their individual components. Serine 18-24 AKT serine/threonine kinase 1 Homo sapiens 14-17 35155201-5 2021 As a potential molecular link, CD133-mediated activation of phosphoinositide 3-kinase/serine-threonine kinase (PI3K/AKT) signaling appears to increase the NRF2 protein levels via phosphorylation and the consequent inhibition of glycogen synthase kinase (GSK)-3beta. Serine 86-92 AKT serine/threonine kinase 1 Homo sapiens 116-119 35330934-1 2022 The serine/threonine kinase Akt, also known as protein kinase B (PKB), is one of the key factors regulating glucose and lipid energy metabolism, and is the core focus of current research on diabetes and metabolic diseases. Serine 4-10 AKT serine/threonine kinase 1 Homo sapiens 28-31 35402577-11 2022 Western blot analysis revealed that circulating exosome miR-21 in HUVECs might weaken negative regulation of phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase (AKT) by target-inhibiting phosphatase and tensin homolog (PTEN) and inducing the expression of hypoxia-inducible factor 1-alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF). Serine 146-152 AKT serine/threonine kinase 1 Homo sapiens 171-174 35111368-2 2022 Serine/threonine kinase (AKT) is a highly recognized antioxidant, immunomodulatory, anti-proliferation, and endocrine modulatory molecule. Serine 0-6 AKT serine/threonine kinase 1 Homo sapiens 25-28 34985995-0 2022 A Pseudorabies Virus Serine/Threonine Kinase, US3, Promotes Retrograde Transport in Axons via Akt/mToRC1. Serine 21-27 AKT serine/threonine kinase 1 Homo sapiens 94-97 34985995-15 2022 The US3 serine/threonine kinase of PRV activates Akt-mToRC1 signaling pathways early during infection to promote axonal translation. Serine 8-14 AKT serine/threonine kinase 1 Homo sapiens 49-52 35016518-1 2022 OBJECTIVE: To explore the role of phosphatidylinositol-3-kinase/protein kinase B and alpha serine/threonine protein kinase PI3K/PKB (also known as PI3K/Akt) signaling pathway in liver ischemia reperfusion injury. Serine 91-97 AKT serine/threonine kinase 1 Homo sapiens 128-131 35125881-8 2022 The mutation of the PIK3CA gene was also found to lead to increased expression of serine-threonine kinase (AKT) in adipocytes in the PI3K-AKT-mTOR signaling pathway. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 107-110 35125881-8 2022 The mutation of the PIK3CA gene was also found to lead to increased expression of serine-threonine kinase (AKT) in adipocytes in the PI3K-AKT-mTOR signaling pathway. Serine 82-88 AKT serine/threonine kinase 1 Homo sapiens 138-141 35072776-2 2022 Increased glycolysis is one of the most common cellular adaptations to hypoxia and mostly is regulated via hypoxia-inducible factor (HIF) and RAC-alpha serine/threonine-protein kinase (Akt) signaling, which gets activated under reduced oxygen content. Serine 152-158 AKT serine/threonine kinase 1 Homo sapiens 185-188 35056723-9 2022 Contrastingly, the upregulation of PP2A-A subunit expression may have modulated the suppression of various cell survival proteins such as p-Akt (Ser 473), FoxO3a and beta-catenin. Serine 145-148 AKT serine/threonine kinase 1 Homo sapiens 140-143 34907409-6 2022 The underlying molecular mechanism lies in the activation of the integrin alpha2-PI3K-Akt signaling pathway, where the SLM-1MAH surface increased the protein expressions of integrin alpha2 (Itga2), phosphatidylinositol 3-kinase (PI3K), and phosphorylated serine/threonine kinase Akt (p-Akt) to enhance osteogenesis and osseointegration. Serine 255-261 AKT serine/threonine kinase 1 Homo sapiens 86-89 34907409-6 2022 The underlying molecular mechanism lies in the activation of the integrin alpha2-PI3K-Akt signaling pathway, where the SLM-1MAH surface increased the protein expressions of integrin alpha2 (Itga2), phosphatidylinositol 3-kinase (PI3K), and phosphorylated serine/threonine kinase Akt (p-Akt) to enhance osteogenesis and osseointegration. Serine 255-261 AKT serine/threonine kinase 1 Homo sapiens 279-282 34907409-6 2022 The underlying molecular mechanism lies in the activation of the integrin alpha2-PI3K-Akt signaling pathway, where the SLM-1MAH surface increased the protein expressions of integrin alpha2 (Itga2), phosphatidylinositol 3-kinase (PI3K), and phosphorylated serine/threonine kinase Akt (p-Akt) to enhance osteogenesis and osseointegration. Serine 255-261 AKT serine/threonine kinase 1 Homo sapiens 284-289 35161226-8 2022 SKAFAb increased sprout outgrowth and increased the phosphorylations of serine/threonine-specific protein kinase (Akt), c-Jun NH2-terminal kinase, extracellular signal-regulated kinase1/2, and p38 mitogen-activated protein kinase (MAPK) in HaCaT cells. Serine 72-78 AKT serine/threonine kinase 1 Homo sapiens 114-117 35069784-8 2022 NUCKS1, a positive regulator of phosphatidylinositol-3-kinases (PI3K)/protein-serine-threonine kinase (AKT) pathway, was confirmed as a direct target of miR-641. Serine 78-84 AKT serine/threonine kinase 1 Homo sapiens 103-106