PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11279203-13 2001 In addition, we identify a beta-arrestin mutant with enhanced affinity for the agonist-activated beta2-adrenergic receptor that traffics into endocytic vesicles with receptors that lack serine/threonine clusters and normally dissociate from wild-type beta-arrestin at the plasma membrane. Serine 186-192 adrenoceptor beta 2 Homo sapiens 97-122 10964911-1 2000 A critical role for Ser-2035.42 in ligand binding to and activation of the beta 2-adrenergic receptor. Serine 20-23 adrenoceptor beta 2 Homo sapiens 75-101 11278469-8 2001 Abrogation of the phosphorylation of serine 685 on GRK2 by mutagenesis (S685A) or by expression of a dominant negative AKAP79 mutant reduces GRK2-mediated translocation to beta2AR and phosphorylation of agonist-occupied beta2AR, thus reducing subsequent receptor internalization. Serine 37-43 adrenoceptor beta 2 Homo sapiens 172-179 11278469-8 2001 Abrogation of the phosphorylation of serine 685 on GRK2 by mutagenesis (S685A) or by expression of a dominant negative AKAP79 mutant reduces GRK2-mediated translocation to beta2AR and phosphorylation of agonist-occupied beta2AR, thus reducing subsequent receptor internalization. Serine 37-43 adrenoceptor beta 2 Homo sapiens 220-227 11146000-2 2001 In this study, we investigated the role that the carboxyl tail PKA site (Ser(345,346)) of the beta(2)AR plays in its rapid agonist-promoted phosphorylation and desensitization. Serine 73-76 adrenoceptor beta 2 Homo sapiens 94-103 11146000-7 2001 Taken with the observation that mutation of either Ser(345,346) or of the beta ARK phosphorylation sites prevented both the hyper-phosphorylation and constitutive desensitization of a palmitoylation-less mutant (Gly(341)beta(2)AR), our data suggest a concerted/synergistic action of the two kinases that depends on the palmitoylation state of the receptor. Serine 51-54 adrenoceptor beta 2 Homo sapiens 220-229 10617695-1 2000 The binding free energy for the interaction between serines 204 and 207 of the fifth transmembrane helix of the beta(2)-adrenergic receptor (beta(2)-AR) and catecholic hydroxyl (OH) groups of adrenergic agonists was analyzed using double mutant cycles. Serine 52-59 adrenoceptor beta 2 Homo sapiens 112-139 11040066-3 2000 In this study, we examined the role of the carboxyl terminal serines, 355, 356, and 364, in the GRK-mediated desensitization of the betaAR. Serine 61-68 adrenoceptor beta 2 Homo sapiens 132-138 11040066-14 2000 Our data demonstrate that the cluster of serines within the 355 to 364 betaAR domain confer the rapid, GRK-mediated, receptor-level desensitization of the betaAR. Serine 41-48 adrenoceptor beta 2 Homo sapiens 71-77 11040066-14 2000 Our data demonstrate that the cluster of serines within the 355 to 364 betaAR domain confer the rapid, GRK-mediated, receptor-level desensitization of the betaAR. Serine 41-48 adrenoceptor beta 2 Homo sapiens 155-161 10617695-1 2000 The binding free energy for the interaction between serines 204 and 207 of the fifth transmembrane helix of the beta(2)-adrenergic receptor (beta(2)-AR) and catecholic hydroxyl (OH) groups of adrenergic agonists was analyzed using double mutant cycles. Serine 52-59 adrenoceptor beta 2 Homo sapiens 141-151 9309797-1 1997 Molecular modeling studies have predicted that the beta-hydroxyl group of the catecholamines interacts with the beta 2-adrenoceptor at the serine residue at position 165 (Ser165) located on transmembrane helix IV; however, this has not been confirmed by site-directed mutagenesis. Serine 139-145 adrenoceptor beta 2 Homo sapiens 112-131 10499588-6 1999 A serine residue at position 411 in the tail of the beta2-adrenergic receptor is a substrate for phosphorylation by GRK-5 (for G-protein-coupled-receptor kinase-5) and is required for interaction with EBP50 and for proper recycling of the receptor. Serine 2-8 adrenoceptor beta 2 Homo sapiens 52-77 1848190-3 1991 Mutation of the serine-261, -262, -344 and -345 of the beta 2-adrenergic receptor prevented the phorbol-ester-induced phosphorylation of the receptor. Serine 16-22 adrenoceptor beta 2 Homo sapiens 55-81 8662852-8 1996 Increasing the stoichiometry of GRK2-mediated beta2AR phosphorylation from approximately 1.0 to 5.0 mol Pi/mol receptor increases the stoichiometry of phosphorylation of Thr-384, Ser-396, Ser-401, and Ser-407 rather than increasing the number of phosphoacceptor sites. Serine 188-191 adrenoceptor beta 2 Homo sapiens 46-53 1849641-6 1991 We replaced four serine and threonine residues located within a 10-amino acid segment of this domain of beta 2AR and thereby prevented agonist-promoted phosphorylation, sequestration, and rapid desensitization of the adenylyl cyclase response. Serine 17-23 adrenoceptor beta 2 Homo sapiens 104-112 8702933-2 1996 In the present study, we show that additional mutation of serines 345 and 346 (Ala345,346Gly341beta2AR) restored normal phosphorylation and receptor-Gs coupling, thus suggesting that the increased phosphorylation of this site, rather than the lack of palmitoylation per se, is responsible for the poor coupling of the unpalmitoylated receptor. Serine 58-65 adrenoceptor beta 2 Homo sapiens 95-102 8662852-8 1996 Increasing the stoichiometry of GRK2-mediated beta2AR phosphorylation from approximately 1.0 to 5.0 mol Pi/mol receptor increases the stoichiometry of phosphorylation of Thr-384, Ser-396, Ser-401, and Ser-407 rather than increasing the number of phosphoacceptor sites. Serine 179-182 adrenoceptor beta 2 Homo sapiens 46-53 8662852-8 1996 Increasing the stoichiometry of GRK2-mediated beta2AR phosphorylation from approximately 1.0 to 5.0 mol Pi/mol receptor increases the stoichiometry of phosphorylation of Thr-384, Ser-396, Ser-401, and Ser-407 rather than increasing the number of phosphoacceptor sites. Serine 188-191 adrenoceptor beta 2 Homo sapiens 46-53 2836733-6 1988 Here we report that prevention of agonist-stimulated beta 2-adrenergic receptor (beta 2AR) phosphorylation by truncation of its serine and threonine-rich phosphate acceptor segment delays the onset of desensitization. Serine 128-134 adrenoceptor beta 2 Homo sapiens 53-79 2836733-6 1988 Here we report that prevention of agonist-stimulated beta 2-adrenergic receptor (beta 2AR) phosphorylation by truncation of its serine and threonine-rich phosphate acceptor segment delays the onset of desensitization. Serine 128-134 adrenoceptor beta 2 Homo sapiens 81-89 32067279-6 2020 In our study, we found that Abeta-M expression slowly induced cell apoptosis within 48 hours after transfection, beta2 adrenergic receptor (beta2AR) interacted with Abeta-M in the pull-down and the yeast two-hybrid assays, and Abeta-M played a major role in inducing phosphorylation of Tau at Ser-214, c-Jun N-terminal kinase (JNK) at Thr-183/Tyr-185, p70 ribosomal protein S6 kinase (p70S6K) at Thr-389. Serine 293-296 adrenoceptor beta 2 Homo sapiens 113-138 29393391-8 2018 The levels of angiotensin II receptor type 1b (Agtr1b), Agtr2 and Agt were markedly upregulated and the expression of beta2-AR phospho-Ser(355,356) steadily decreased in the right heart. Serine 135-138 adrenoceptor beta 2 Homo sapiens 118-126 21868357-4 2011 The beta(2)-adrenergic receptor (beta(2)AR) has many serine and threonine residues in the carboxyl-terminal tail and the intracellular loops, which are potential sites of phosphorylation. Serine 53-59 adrenoceptor beta 2 Homo sapiens 4-31 27481942-2 2016 Basal S-palmitoylation of Cys-265 is negligible, but agonist-induced beta2AR activation results in enhanced S-palmitoylation, which requires phosphorylation by the cAMP-dependent protein kinase of Ser-261/Ser-262. Serine 197-200 adrenoceptor beta 2 Homo sapiens 69-76 27481942-2 2016 Basal S-palmitoylation of Cys-265 is negligible, but agonist-induced beta2AR activation results in enhanced S-palmitoylation, which requires phosphorylation by the cAMP-dependent protein kinase of Ser-261/Ser-262. Serine 205-208 adrenoceptor beta 2 Homo sapiens 69-76 26902407-2 2016 Internalization of beta2AR is initiated by phosphorylations of certain serines and threonines by cAMP dependent protein kinase A (PKA) and G protein-coupled receptor kinases (GRK). Serine 71-78 adrenoceptor beta 2 Homo sapiens 19-26 25666616-4 2015 Upon beta2AR activation, a specific isoform of the second messenger cAMP-dependent protein kinase A (PKAalpha) rapidly phosphorylates USP20 on serine 333 located in its unique insertion domain. Serine 143-149 adrenoceptor beta 2 Homo sapiens 5-12 27169346-4 2016 The protein kinase activity of PI3K phosphorylates serine residue 70 on Src to enhance its activity and induce EGFR transactivation following betaAR stimulation. Serine 51-57 adrenoceptor beta 2 Homo sapiens 142-148 21868357-4 2011 The beta(2)-adrenergic receptor (beta(2)AR) has many serine and threonine residues in the carboxyl-terminal tail and the intracellular loops, which are potential sites of phosphorylation. Serine 53-59 adrenoceptor beta 2 Homo sapiens 33-42 18056263-7 2008 Subsequent studies showed that the Epi-activated beta(2)AR underwent a rapid phosphorylation by G protein-coupled receptor kinase 2 (GRK2) and subsequent dephosphorylation on serine residues 355 and 356, which was critical for sufficient receptor recycling and G(i) coupling. Serine 175-181 adrenoceptor beta 2 Homo sapiens 49-58 21419339-4 2011 Mechanistically, PI3Kgamma inhibits PP2A activity at the betaAR complex by phosphorylating an intracellular inhibitor of PP2A (I2PP2A) on serine residues 9 and 93, resulting in enhanced binding to PP2A. Serine 138-144 adrenoceptor beta 2 Homo sapiens 57-63 21225244-7 2011 A 5-min treatment with NAD decreased forskolin-stimulated phosphorylation at the beta(2)AR PKA site Ser 262 while a 24-h treatment with NAD increased it. Serine 100-103 adrenoceptor beta 2 Homo sapiens 81-90 20345371-7 2010 beta(2)AR-TRHR was phosphorylated well at the PKA site in the third intracellular loop, but poorly at GRK sites in the tail, whereas TRHR-beta(2)AR was phosphorylated strongly at GRK sites in the tail (Ser(355)/Ser(356) of the beta(2)AR). Serine 202-205 adrenoceptor beta 2 Homo sapiens 0-9 20345371-7 2010 beta(2)AR-TRHR was phosphorylated well at the PKA site in the third intracellular loop, but poorly at GRK sites in the tail, whereas TRHR-beta(2)AR was phosphorylated strongly at GRK sites in the tail (Ser(355)/Ser(356) of the beta(2)AR). Serine 211-214 adrenoceptor beta 2 Homo sapiens 0-9 18056263-10 2008 Together, our data revealed that distinct temporal phosphorylation of beta(2)AR on serine 355 and 356 by GRK2 plays a critical role for dictating receptor cellular events and signaling properties induced by Epi or NE in cardiomyocytes. Serine 83-89 adrenoceptor beta 2 Homo sapiens 70-79 16551744-4 2006 The predicted structures for apo-beta2AR and butoxamine-beta2AR are stable in MD, but in epinephrine-beta2AR, extracellular water trickles into the binding pocket to mediate hydrogen bonding between the catechol of epinephrine and Ser-204 on helix 5. Serine 231-234 adrenoceptor beta 2 Homo sapiens 33-40 16873402-7 2006 beta(2)AR activation also increased NOS activity and phosphorylation of NOS-3 on serine-1177, and these increases were attenuated by inhibition of protein kinase A (PKA), phosphatidylinositol 3-kinase (PI3K) or Akt, and abolished by coinhibition of PKA and Akt. Serine 81-87 adrenoceptor beta 2 Homo sapiens 0-9 16873402-8 2006 These findings suggest that beta(2)AR-mediated NOS-3 activation in HUVEC is mediated through phosphorylation of NOS-3 on serine-1177 through both the PKA and the PI3K/Akt systems, and is sustained by an increase in l-arginine uptake resulting from NO-mediated membrane hyperpolarization. Serine 121-127 adrenoceptor beta 2 Homo sapiens 28-37 16551744-4 2006 The predicted structures for apo-beta2AR and butoxamine-beta2AR are stable in MD, but in epinephrine-beta2AR, extracellular water trickles into the binding pocket to mediate hydrogen bonding between the catechol of epinephrine and Ser-204 on helix 5. Serine 231-234 adrenoceptor beta 2 Homo sapiens 56-63 16551744-4 2006 The predicted structures for apo-beta2AR and butoxamine-beta2AR are stable in MD, but in epinephrine-beta2AR, extracellular water trickles into the binding pocket to mediate hydrogen bonding between the catechol of epinephrine and Ser-204 on helix 5. Serine 231-234 adrenoceptor beta 2 Homo sapiens 56-63 16551744-5 2006 The epinephrine-beta2AR structure shows dynamic flexibility with small, piston-like movements of helices 3 and 6 and transient interhelical hydrogen bonding between Ser-165 on transmembrane 4 and Ser-207 on transmembrane 5. Serine 165-168 adrenoceptor beta 2 Homo sapiens 16-23 16551744-5 2006 The epinephrine-beta2AR structure shows dynamic flexibility with small, piston-like movements of helices 3 and 6 and transient interhelical hydrogen bonding between Ser-165 on transmembrane 4 and Ser-207 on transmembrane 5. Serine 196-199 adrenoceptor beta 2 Homo sapiens 16-23 12167654-4 2002 In the beta(2) adrenergic receptor, mutation of Cys(6.47) to Thr, which in an alpha-helix has a different rotamer distribution from Cys and Ser, produced a constitutively active receptor, whereas the Ser mutant was similar to wild-type receptor. Serine 140-143 adrenoceptor beta 2 Homo sapiens 7-34 16407241-0 2006 Role of the G protein-coupled receptor kinase site serine cluster in beta2-adrenergic receptor internalization, desensitization, and beta-arrestin translocation. Serine 51-57 adrenoceptor beta 2 Homo sapiens 69-94 15845544-1 2005 We converted Ser-207, located in helix 5 of the beta2-adrenergic receptor, into all other natural amino acids. Serine 13-16 adrenoceptor beta 2 Homo sapiens 48-73 15749741-5 2005 Moreover, TARC was able to induce Src-dependent serine phosphorylation of the beta(2)-AR as well as its association with GRK2 and beta-arrestin. Serine 48-54 adrenoceptor beta 2 Homo sapiens 78-88 12167654-4 2002 In the beta(2) adrenergic receptor, mutation of Cys(6.47) to Thr, which in an alpha-helix has a different rotamer distribution from Cys and Ser, produced a constitutively active receptor, whereas the Ser mutant was similar to wild-type receptor. Serine 200-203 adrenoceptor beta 2 Homo sapiens 7-34