PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32681941-8 2020 In terms of mechanism, 6-Gingerol potently suppressed phosphorylation of serine-threonine protein kinase (Akt) - mammalian target of rapamycin (mTOR) - signal transducer and activator of transcription 3 (STAT3) in LPS-treated microglia. Serine 73-79 signal transducer and activator of transcription 3 Homo sapiens 152-202 33003453-3 2020 STAT3 undergoes diverse post-translational modifications, such as the oxidation of cysteine by oxidative stress, the acetylation of lysine, or the phosphorylation of serine/threonine. Serine 166-172 signal transducer and activator of transcription 3 Homo sapiens 0-5 33098871-4 2021 Cdk5 activity was concomitantly induced from injured nerve and increased the phosphorylation of signal transducer and activator of transcription 3 (STAT3) on the serine 727 residue. Serine 162-168 signal transducer and activator of transcription 3 Homo sapiens 96-146 33098871-4 2021 Cdk5 activity was concomitantly induced from injured nerve and increased the phosphorylation of signal transducer and activator of transcription 3 (STAT3) on the serine 727 residue. Serine 162-168 signal transducer and activator of transcription 3 Homo sapiens 148-153 33639070-13 2021 It inhibits STAT3 activation by decreasing STAT3 phosphorylation at serine 727. Serine 68-74 signal transducer and activator of transcription 3 Homo sapiens 12-17 33639070-13 2021 It inhibits STAT3 activation by decreasing STAT3 phosphorylation at serine 727. Serine 68-74 signal transducer and activator of transcription 3 Homo sapiens 43-48 33313091-7 2020 HS-5 cell-induced upregulation of OXPHOS is dependent on the activation of STAT3, especially on that of mitochondrial serine phosphorylated STAT3 (pS-STAT3) in AML cells. Serine 118-124 signal transducer and activator of transcription 3 Homo sapiens 140-145 33313091-7 2020 HS-5 cell-induced upregulation of OXPHOS is dependent on the activation of STAT3, especially on that of mitochondrial serine phosphorylated STAT3 (pS-STAT3) in AML cells. Serine 118-124 signal transducer and activator of transcription 3 Homo sapiens 140-145 32681941-8 2020 In terms of mechanism, 6-Gingerol potently suppressed phosphorylation of serine-threonine protein kinase (Akt) - mammalian target of rapamycin (mTOR) - signal transducer and activator of transcription 3 (STAT3) in LPS-treated microglia. Serine 73-79 signal transducer and activator of transcription 3 Homo sapiens 204-209 32201262-0 2020 Constitutive STAT3 serine phosphorylation promotes Helicobacter-mediated gastric disease. Serine 19-25 signal transducer and activator of transcription 3 Homo sapiens 13-18 32803097-6 2020 As a readout of the variant, serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) was detected only in the nucleus of adrenocortical adenoma component but not in the pheochromocytoma component. Serine 29-35 signal transducer and activator of transcription 3 Homo sapiens 55-105 32803097-6 2020 As a readout of the variant, serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) was detected only in the nucleus of adrenocortical adenoma component but not in the pheochromocytoma component. Serine 29-35 signal transducer and activator of transcription 3 Homo sapiens 107-112 32201262-3 2020 By contrast, the transcriptional role of STAT3 serine phosphorylation (pS), which promotes STAT3-driven mitochondrial activities, is unclear. Serine 47-53 signal transducer and activator of transcription 3 Homo sapiens 41-46 32201262-3 2020 By contrast, the transcriptional role of STAT3 serine phosphorylation (pS), which promotes STAT3-driven mitochondrial activities, is unclear. Serine 47-53 signal transducer and activator of transcription 3 Homo sapiens 91-96 31398350-8 2019 Mechanically, p66Shc knockdown inhibited phosphorylation of STAT3 on serine 727 in vitro and in vivo. Serine 69-75 signal transducer and activator of transcription 3 Homo sapiens 60-65 32192776-3 2020 Herein, it was discovered that silencing NME4 can lead to the marked downregulation of PD-L1, with phosphorylated STAT3 at the 705th serine being inactivated in vitro in esophageal squamous cell carcinoma (ESCC) cell lines. Serine 133-139 signal transducer and activator of transcription 3 Homo sapiens 114-119 31481496-0 2019 Serine-phosphorylated STAT3 promotes tumorigenesis via modulation of RNA polymerase transcriptional activity. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 22-27 31481496-2 2019 By contrast, serine phosphorylation (pS) of STAT3 can augment its nuclear transcriptional activity and promote essential mitochondrial functions, yet the role of pS-STAT3 among epithelial cancers is ill-defined. Serine 13-19 signal transducer and activator of transcription 3 Homo sapiens 44-49 32114280-8 2020 At the molecular level, curcumin increased STAT3 serine 727 phosphorylation intensity and p-STAT3(+) CD4(+) T cells in patients with PsA and psoriasis. Serine 49-55 signal transducer and activator of transcription 3 Homo sapiens 43-48 31451482-6 2019 Mechanistically, MERTK regulated MDSC suppression and differentiation in part through regulation of STAT3 serine phosphorylation and nuclear localization. Serine 106-112 signal transducer and activator of transcription 3 Homo sapiens 100-105 30428347-7 2018 Thpo-regulated mitochondrial activity associated with mitochondrial translocation of STAT3 phosphorylated at serine 727. Serine 109-115 signal transducer and activator of transcription 3 Homo sapiens 85-90 30466966-13 2019 CONCLUSIONS: STAT3 (Ser 727) expression in the nucleus of the ccRCC cells can be a prognostic marker and an antiangiogenic-resistance marker. Serine 20-23 signal transducer and activator of transcription 3 Homo sapiens 13-18 30920934-5 2019 Substitution of phenylalanine for serine increased affinity for IFNAR2 ~4-fold and commensurately enhanced activation of STAT1, STAT3, and STAT5, transcription of a subset of interferon stimulated genes, and restriction of vesicular stomatitis virus infection in vitro. Serine 34-40 signal transducer and activator of transcription 3 Homo sapiens 128-133 30776726-8 2019 By studying intracellular mechanisms triggered by IL-10 and IFN-gamma, we demonstrated that they specifically induced PI3K-dependent serine-phosphorylation of signal transducer and activator of transcription (STAT)3 or STAT1, respectively. Serine 133-139 signal transducer and activator of transcription 3 Homo sapiens 159-215 30323145-8 2018 We found that H2O2 treatment activated NLRP3 inflammasomes and decreased phosphorylation of signal transduction and STAT3 serine 727. Serine 122-128 signal transducer and activator of transcription 3 Homo sapiens 116-121 29774125-6 2018 Moreover, we found that STAT3 was constitutively activated in GICs by phosphorylation on both tyrosine (Y705) and serine (S727) residues. Serine 114-120 signal transducer and activator of transcription 3 Homo sapiens 24-29 30208623-5 2018 STAT3 activation can be regulated, either positively or negatively, by different posttranslational mechanisms including serine or tyrosine phosphorylation/dephosphorylation, acetylation, or demethylation. Serine 120-126 signal transducer and activator of transcription 3 Homo sapiens 0-5 29683208-10 2018 In addition, downstream of ERK, CMC2.24 inhibited STAT3 phosphorylation levels at the serine 727 residue, enhanced the levels of superoxide anion in mitochondria, and induced intrinsic apoptosis as shown by the release of cytochrome c from the mitochondria to the cytosol and the further cleavage of caspase 9 in PC cells. Serine 86-92 signal transducer and activator of transcription 3 Homo sapiens 50-55 30263097-4 2018 Using Western immunoblotting, immunoprecipitation, and flow cytometry we found that, apart from its constitutive serine phosphorylation, STAT3 is constitutively acetylated on lysine 685 residues. Serine 113-119 signal transducer and activator of transcription 3 Homo sapiens 137-142 29488000-0 2018 Bilateral activation of STAT3 by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and its nuclear translocation in primary sensory neurons following unilateral sciatic nerve injury. Serine 80-86 signal transducer and activator of transcription 3 Homo sapiens 24-29 29488000-2 2018 STAT3 activation was by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and was followed by their nuclear translocation. Serine 71-77 signal transducer and activator of transcription 3 Homo sapiens 0-5 29212252-7 2017 Pharmacological inhibition and short hairpin RNA-mediated knockdown experiments showed that AKT-dependent mammalian target of rapamycin (mTOR) activation was involved in the phosphorylation of serine-727 of STAT3, which in turn stimulated the transcription of the CCR1 gene. Serine 193-199 signal transducer and activator of transcription 3 Homo sapiens 207-212 29652915-8 2018 PRKD3 silencing was also shown to reduce serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) as well as phosphorylation of all three mitogen-activated protein kinase groups. Serine 41-47 signal transducer and activator of transcription 3 Homo sapiens 67-117 29652915-8 2018 PRKD3 silencing was also shown to reduce serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) as well as phosphorylation of all three mitogen-activated protein kinase groups. Serine 41-47 signal transducer and activator of transcription 3 Homo sapiens 119-124 29431732-5 2018 Specifically, blocking AhR redirected IFN-beta signaling to STAT3 phosphorylation through both tyrosine and serine sites, which subsequently facilitated STAT3 nuclear translocation and subsequent binding to the p53 promoter in the nucleus. Serine 108-114 signal transducer and activator of transcription 3 Homo sapiens 60-65 29431732-5 2018 Specifically, blocking AhR redirected IFN-beta signaling to STAT3 phosphorylation through both tyrosine and serine sites, which subsequently facilitated STAT3 nuclear translocation and subsequent binding to the p53 promoter in the nucleus. Serine 108-114 signal transducer and activator of transcription 3 Homo sapiens 153-158 28130399-1 2017 In chronic lymphocytic leukemia (CLL), STAT3 is constitutively phosphorylated on serine 727 and plays a role in the pathobiology of CLL. Serine 81-87 signal transducer and activator of transcription 3 Homo sapiens 39-44 28968425-11 2017 Among these molecules, the protein levels of STAT3 were greatly enhanced in all hepatocyte nuclei and further elevated in the cytoplasm in HCC tissue samples at 18 months, and the levels of phosphorylated TP53 (p-p53-Ser 6 and -Ser 15) were increased in liver tissues. Serine 217-220 signal transducer and activator of transcription 3 Homo sapiens 45-50 28968425-11 2017 Among these molecules, the protein levels of STAT3 were greatly enhanced in all hepatocyte nuclei and further elevated in the cytoplasm in HCC tissue samples at 18 months, and the levels of phosphorylated TP53 (p-p53-Ser 6 and -Ser 15) were increased in liver tissues. Serine 228-231 signal transducer and activator of transcription 3 Homo sapiens 45-50 28781685-0 2017 Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells. Serine 4-10 signal transducer and activator of transcription 3 Homo sapiens 79-129 28542650-6 2017 In addition, significant phosphorylation of STAT1 (ser 727) and STAT3 (both tyr 705 and ser 727 residues) was also observed, accompanied by a decrease in total STAT1. Serine 88-91 signal transducer and activator of transcription 3 Homo sapiens 64-69 28542650-11 2017 These results suggest cross-communication between ERK1/2 and JAK-STAT pathways during EGF-mediated increase in invasion of trophoblast cells; phosphorylation at ser 727 residue of both STAT3 and STAT1 appears to be critical. Serine 161-164 signal transducer and activator of transcription 3 Homo sapiens 185-190 28130399-3 2017 Mass spectrometry was used to identify casein kinase 2 (CK2), a serine/threonine kinase that coimmunoprecipitated with serine phosphorylated STAT3 (pSTAT3). Serine 64-70 signal transducer and activator of transcription 3 Homo sapiens 141-146 28130399-4 2017 Furthermore, activated CK2 incubated with recombinant STAT3 induced phosphorylation of STAT3 on serine 727. Serine 96-102 signal transducer and activator of transcription 3 Homo sapiens 54-59 28130399-4 2017 Furthermore, activated CK2 incubated with recombinant STAT3 induced phosphorylation of STAT3 on serine 727. Serine 96-102 signal transducer and activator of transcription 3 Homo sapiens 87-92 28130399-10 2017 Finally, confocal microscopy determined that CD5 is cell membrane bound, and fractionation studies revealed that the CK2/CD5/BLNK/STAT3 complex remains in the cytoplasm, whereas serine pSTAT3 is shuttled to the nucleus.Implications: These data show that the cellular proteins CK2, CD5, and BLNK are required for constitutive phosphorylation of STAT3 in CLL. Serine 178-184 signal transducer and activator of transcription 3 Homo sapiens 186-191 26138072-0 2015 A Resveratrol Analogue Promotes ERKMAPK-Dependent Stat3 Serine and Tyrosine Phosphorylation Alterations and Antitumor Effects In Vitro against Human Tumor Cells. Serine 56-62 signal transducer and activator of transcription 3 Homo sapiens 50-55 28188292-7 2017 Additionally, we observed a novel cytosolic DNA-induced phosphorylation at serine 754 in the transactivation domain of STAT3. Serine 75-81 signal transducer and activator of transcription 3 Homo sapiens 119-124 27966451-3 2017 Consistent with their enhanced growth properties, FGFR4-R388-expressing cells show higher mitochondrial STAT3 serine phosphorylation driving basal and maximal oxygen consumption rate (OCR) than pituitary cells expressing the more common FGFR4-G388 isoform. Serine 110-116 signal transducer and activator of transcription 3 Homo sapiens 104-109 27966451-6 2017 The effects on OCR were recapitulated by introducing a constitutively active serine STAT3 but not by a tyrosine-active mutant. Serine 77-83 signal transducer and activator of transcription 3 Homo sapiens 84-89 27502766-4 2016 However, the role of serine phosphorylation of STAT3 (P-STAT3 Ser) is in large part undetermined. Serine 21-27 signal transducer and activator of transcription 3 Homo sapiens 47-52 27064016-6 2016 RESULTS: We found that HN3 and FaDu cells expressed strongly phosphorylated STAT3 on both tyrosine 705 and serine 727 residues as compared to other SCCHN cells. Serine 107-113 signal transducer and activator of transcription 3 Homo sapiens 76-81 26783739-0 2016 STAT3-Ser/Hes3 Signaling: A New Molecular Component of the Neuroendocrine System? Serine 6-9 signal transducer and activator of transcription 3 Homo sapiens 0-5 26783739-7 2016 The STAT3-Ser/Hes3 Signaling Axis was first identified as a major regulator of neural stem cells and, subsequently, cancer stem cells. Serine 10-13 signal transducer and activator of transcription 3 Homo sapiens 4-9 27127727-5 2015 Following recent observations of differences in mitochondrially localized serine 727 phosphorylated STAT3 (mtSTAT3-pS727) in preadipocytes and adipocytes, here, we hypothesize and speculate further on the role of mitochondrial STAT3 in adipogenesis. Serine 74-80 signal transducer and activator of transcription 3 Homo sapiens 100-105 27127727-5 2015 Following recent observations of differences in mitochondrially localized serine 727 phosphorylated STAT3 (mtSTAT3-pS727) in preadipocytes and adipocytes, here, we hypothesize and speculate further on the role of mitochondrial STAT3 in adipogenesis. Serine 74-80 signal transducer and activator of transcription 3 Homo sapiens 109-114 28188289-7 2017 UTP increased the phosphorylation of p38, ERK, CREB, and Ser-727 of STAT3 and induced nuclear translocation of pCaMKII. Serine 57-60 signal transducer and activator of transcription 3 Homo sapiens 68-73 26705936-7 2017 MCF-7 cells cocultured with M2 TAM showed activated JAK1/STAT3 and Raf/MEK/JNK pathways contributing to tyrosine and serine phophorylation of STAT3, respectively. Serine 117-123 signal transducer and activator of transcription 3 Homo sapiens 142-147 27502766-7 2016 Subcellular fractionation further revealed that P-STAT3 Ser was localized in mitochondria. Serine 56-59 signal transducer and activator of transcription 3 Homo sapiens 50-55 27502766-8 2016 Overexpression of SOCS3 with a Lentivirus-mediated approach in SH-SY5Y cells inhibited OSM-induced P-STAT3 Ser in both cytosol and mitochondria fractions. Serine 107-110 signal transducer and activator of transcription 3 Homo sapiens 101-106 27502766-9 2016 In contrast, OSM-induced P-STAT3 Ser was further upregulated in both cytosol and mitochondria when SOCS3 was knocked down by Lentivirus-delivered shSOCS3. Serine 33-36 signal transducer and activator of transcription 3 Homo sapiens 27-32 27502766-10 2016 Using a rat T8 spinal cord complete transection model, we found that SCI induced upregulation of P-STAT3 Ser in the mitochondria of macrophages/microglia and neurons both rostral and caudal to the injury site of spinal cord. Serine 105-108 signal transducer and activator of transcription 3 Homo sapiens 99-104 26727576-3 2016 In ABC DLBCL cells, eosinophils and perhaps all cells, four variant STAT3 mRNAs (Salpha, DeltaSalpha, Sbeta and DeltaSbeta) are present as a result of two alternative splicing events, one that results in the inclusion of a 55-residue C-terminal transactivation domain (alpha) or a truncated C-terminal domain with 7 unique residues (beta) and a second that includes (S) or excludes (DeltaS) the codon for Ser-701 in the linker between the SH2 and C-terminal domains. Serine 405-408 signal transducer and activator of transcription 3 Homo sapiens 68-73 25736961-0 2016 STAT3 Serine 727 Phosphorylation: A Relevant Target to Radiosensitize Human Glioblastoma. Serine 6-12 signal transducer and activator of transcription 3 Homo sapiens 0-5 25736961-5 2016 Here, we studied the role of STAT3 activation on tyrosine 705 (Y705) and serine 727 (S727) in glioma radioresistance. Serine 73-79 signal transducer and activator of transcription 3 Homo sapiens 29-34 26330924-0 2015 Prognostic Significance of Serine-Phosphorylated STAT3 Expression in pT1-T2 Oral Tongue Carcinoma. Serine 27-33 signal transducer and activator of transcription 3 Homo sapiens 49-54 26330924-8 2015 CONCLUSION: In spite of these results, it is worth further investigating the role of pSTAT3 (serine- and tyrosine-pSTAT3) in oral tongue SCC in larger series because preclinical models are increasingly showing that several anticancer strategies would benefit from STAT3 phosphorylation inhibition. Serine 93-99 signal transducer and activator of transcription 3 Homo sapiens 86-91 26045618-3 2015 STAT3 phosphorylated at serine 727 (Ser727) localizes in mitochondria, but little is known about mitochondrial STAT3"s contribution to carcinogenesis in Barrett"s esophagus, which is the focus of this study. Serine 24-30 signal transducer and activator of transcription 3 Homo sapiens 0-5 26106584-3 2015 STAT3 can, however, also localize to mitochondria, where its serine-phosphorylated (S-P) form preserves mitochondrial oxidative phosphorylation and controls the opening of the mitochondrial permeability transition pore, also promoting survival and resistance to apoptosis in response to specific signals/oncogenes such as RAS. Serine 61-67 signal transducer and activator of transcription 3 Homo sapiens 0-5 25383546-5 2014 The mechanism of TBr mediated inhibition of p-STAT3 was found to be due to the activation of ubiquitin dependent degradation of tyrosine 705 and serine 727 p-STAT3. Serine 145-151 signal transducer and activator of transcription 3 Homo sapiens 46-51 25584415-0 2015 Serine-727 phosphorylation activates hypothalamic STAT-3 independently from tyrosine-705 phosphorylation. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 50-56 25584415-3 2015 In nonhypothalamic cells, STAT-3 is also phosphorylated at serine-727 (Ser-727), but the functional significance of Ser-727 in the regulation of hypothalamic STAT-3 is not known. Serine 59-65 signal transducer and activator of transcription 3 Homo sapiens 26-32 25584415-3 2015 In nonhypothalamic cells, STAT-3 is also phosphorylated at serine-727 (Ser-727), but the functional significance of Ser-727 in the regulation of hypothalamic STAT-3 is not known. Serine 71-74 signal transducer and activator of transcription 3 Homo sapiens 26-32 25584415-3 2015 In nonhypothalamic cells, STAT-3 is also phosphorylated at serine-727 (Ser-727), but the functional significance of Ser-727 in the regulation of hypothalamic STAT-3 is not known. Serine 116-119 signal transducer and activator of transcription 3 Homo sapiens 26-32 25584415-3 2015 In nonhypothalamic cells, STAT-3 is also phosphorylated at serine-727 (Ser-727), but the functional significance of Ser-727 in the regulation of hypothalamic STAT-3 is not known. Serine 116-119 signal transducer and activator of transcription 3 Homo sapiens 158-164 25584415-12 2015 Thus, EGF-promoted Ser-727 phosphorylation by ERK-1/2 is not only sufficient to fully activate hypothalamic STAT-3, but, in terms of targeted genes and required cofactors, entails distinct modes of STAT-3 actions compared with IFN-gamma-induced Tyr-705 phosphorylation. Serine 19-22 signal transducer and activator of transcription 3 Homo sapiens 108-114 25584415-12 2015 Thus, EGF-promoted Ser-727 phosphorylation by ERK-1/2 is not only sufficient to fully activate hypothalamic STAT-3, but, in terms of targeted genes and required cofactors, entails distinct modes of STAT-3 actions compared with IFN-gamma-induced Tyr-705 phosphorylation. Serine 19-22 signal transducer and activator of transcription 3 Homo sapiens 198-204 25512602-8 2015 IL-10 induced phosphorylation of both Tyr(705) and Ser(727) residues of STAT3 in an AMPKalpha1-dependent manner, and these phosphorylation events were blocked by inhibition of Ca(2+)/calmodulin-dependent protein kinase kinase beta, an upstream activator of AMPK, and by the mTORC1 inhibitor rapamycin, respectively. Serine 51-54 signal transducer and activator of transcription 3 Homo sapiens 72-77 25383546-5 2014 The mechanism of TBr mediated inhibition of p-STAT3 was found to be due to the activation of ubiquitin dependent degradation of tyrosine 705 and serine 727 p-STAT3. Serine 145-151 signal transducer and activator of transcription 3 Homo sapiens 158-163 25074934-7 2014 In hTCEpi cells, both cell surface and intracellular EGFRs exhibited dose-dependent increases in effector activity after 15 min of ligand stimulation, but only the serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) was statistically significant when accounting for receptor phosphorylation. Serine 164-170 signal transducer and activator of transcription 3 Homo sapiens 190-240 25299776-5 2014 We observed that leukemic but not normal B cells from CLL patients exhibit constitutive activation of an atypical form of the STAT3 signaling factor, phosphorylated on serine 727 (Ser(727)) in the absence of detectable canonical tyrosine 705 (Tyr705)-dependent activation in vivo. Serine 168-174 signal transducer and activator of transcription 3 Homo sapiens 126-131 25299776-5 2014 We observed that leukemic but not normal B cells from CLL patients exhibit constitutive activation of an atypical form of the STAT3 signaling factor, phosphorylated on serine 727 (Ser(727)) in the absence of detectable canonical tyrosine 705 (Tyr705)-dependent activation in vivo. Serine 180-183 signal transducer and activator of transcription 3 Homo sapiens 126-131 25299776-6 2014 The Ser(727)-phosphorylated STAT3 molecule (pSTAT3Ser(727)) is localized to the mitochondria and associates with complex I of the respiratory chain. Serine 4-7 signal transducer and activator of transcription 3 Homo sapiens 28-33 25038288-12 2014 This suggests a specific role of serine-phosphorylated STAT3, independent of tyrosine phosphorylation in the oncogenesis of endometrial cancer. Serine 33-39 signal transducer and activator of transcription 3 Homo sapiens 55-60 24726840-0 2014 Reduced phosphorylation of Stat3 at Ser-727 mediated by casein kinase 2 - protein phosphatase 2A enhances Stat3 Tyr-705 induced tumorigenic potential of glioma cells. Serine 36-39 signal transducer and activator of transcription 3 Homo sapiens 27-32 24726840-0 2014 Reduced phosphorylation of Stat3 at Ser-727 mediated by casein kinase 2 - protein phosphatase 2A enhances Stat3 Tyr-705 induced tumorigenic potential of glioma cells. Serine 36-39 signal transducer and activator of transcription 3 Homo sapiens 106-111 24726840-12 2014 Thus, in glioma, reduced Stat3 Ser-727 phosphorylation enhances tumorigenicity which may be regulated in part by CK2-PP2A pathway. Serine 31-34 signal transducer and activator of transcription 3 Homo sapiens 25-30 24726840-2 2014 However, the role and regulation of Stat3 Ser-727 phosphorylation in cancer cells have not been clearly evaluated. Serine 42-45 signal transducer and activator of transcription 3 Homo sapiens 36-41 24726840-3 2014 In our findings, correlation studies on the expression of CK2 and Stat3 Ser-727 phosphorylation levels in human glioma patient samples as well as rat orthotopic tumor model show a degree of negative correlation. Serine 72-75 signal transducer and activator of transcription 3 Homo sapiens 66-71 24726840-5 2014 Here, increased Stat3 Ser-727 phosphorylation upon CK2 inhibition was observed. Serine 22-25 signal transducer and activator of transcription 3 Homo sapiens 16-21 24726840-6 2014 Overexpression of CK2 (alpha, alpha" or beta subunits) by transient transfection resulted in decreased Stat3 Ser-727 phosphorylation. Serine 109-112 signal transducer and activator of transcription 3 Homo sapiens 103-108 24726840-8 2014 Interestingly, we found PP2A, a protein phosphatase, to be a mediator in the negative regulation of Stat3 Ser-727 phosphorylation by CK2. Serine 106-109 signal transducer and activator of transcription 3 Homo sapiens 100-105 24726840-9 2014 In vitro assays prove that Ser-727 phosphorylation of Stat3 affects the transcriptional activity of its downstream targets like SOCS3, bcl-xl and Cyclin D1. Serine 27-30 signal transducer and activator of transcription 3 Homo sapiens 54-59 25031733-0 2014 STAT3 serine 727 phosphorylation influences clinical outcome in glioblastoma. Serine 6-12 signal transducer and activator of transcription 3 Homo sapiens 0-5 25219839-9 2014 In cultured atrial myocytes and fibroblasts, Ang-II induced tyrosine and serine phosphorylation of STAT3 showing interaction with MMP1 and MMP2 and DNA promoter sequences in atrial fibroblasts. Serine 73-79 signal transducer and activator of transcription 3 Homo sapiens 99-104 25089666-6 2014 Taken together with the recently uncovered role of STAT3 in regulating energy metabolism from within the mitochondrion when phosphorylated on Ser 727, these data place STAT3 at the center of a hub regulating energy metabolism under different conditions, in most cases promoting cell survival, proliferation and malignant transformation even though with distinct mechanisms. Serine 142-145 signal transducer and activator of transcription 3 Homo sapiens 51-56 25089666-6 2014 Taken together with the recently uncovered role of STAT3 in regulating energy metabolism from within the mitochondrion when phosphorylated on Ser 727, these data place STAT3 at the center of a hub regulating energy metabolism under different conditions, in most cases promoting cell survival, proliferation and malignant transformation even though with distinct mechanisms. Serine 142-145 signal transducer and activator of transcription 3 Homo sapiens 168-173 24847057-6 2014 Interestingly, at the same time Ser(P)(727)-STAT3 binding to its response element regions in the HAS2 promoter was unchanged or decreased. Serine 32-35 signal transducer and activator of transcription 3 Homo sapiens 44-49 24582688-1 2014 Our previous studies suggested that arsenic is able to induce serine 21 phosphorylation of the EZH2 protein through activation of JNK, STAT3, and Akt signaling pathways in the bronchial epithelial cell line, BEAS-2B. Serine 62-68 signal transducer and activator of transcription 3 Homo sapiens 135-140 24487064-2 2014 We have shown recently that heteroarylketones (HAKs) interfere with stimulated interleukin-6 expression in astrocytes by suppression of STAT3 phosphorylation at serine 727. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 136-141 24615012-4 2014 Using quantitative liquid chromatography-tandem mass spectrometry, we have discovered and characterized a novel STAT3 phosphoform that is simultaneously phosphorylated at Thr(714) and Ser(727) by glycogen synthase kinase 3alpha and -beta (GSK-3alpha/beta). Serine 184-187 signal transducer and activator of transcription 3 Homo sapiens 112-117 24615012-5 2014 Both Thr(714) and Ser(727) are required for STAT3-dependent gene induction in response to simultaneous activation of epidermal growth factor receptor (EGFR) and protease-activated receptor 1 (PAR-1) in endothelial cells. Serine 18-21 signal transducer and activator of transcription 3 Homo sapiens 44-49 24396070-9 2014 By affecting the subcellular distribution of STAT3, SH2B1beta increased serine phosphorylation and the concomitant transcriptional activity of STAT3. Serine 72-78 signal transducer and activator of transcription 3 Homo sapiens 45-50 23913134-5 2014 Importantly, HDAC3 inhibition downregulates phosphorylation (tyrosine 705 and serine 727) of signal transducers and activators of transcription 3 (STAT3). Serine 78-84 signal transducer and activator of transcription 3 Homo sapiens 93-145 23913134-5 2014 Importantly, HDAC3 inhibition downregulates phosphorylation (tyrosine 705 and serine 727) of signal transducers and activators of transcription 3 (STAT3). Serine 78-84 signal transducer and activator of transcription 3 Homo sapiens 147-152 24625004-5 2014 In contrast, expression of the minor FGFR4-R388 allele enhances STAT3 serine phosphorylation, driving cellular growth. Serine 70-76 signal transducer and activator of transcription 3 Homo sapiens 64-69 24396070-9 2014 By affecting the subcellular distribution of STAT3, SH2B1beta increased serine phosphorylation and the concomitant transcriptional activity of STAT3. Serine 72-78 signal transducer and activator of transcription 3 Homo sapiens 143-148 24389215-6 2014 Tumorigenic cells can make use of distinct, even opposing pathways, including JAK/STAT and the non-canonical STAT3-Ser/Hes3 signaling axis. Serine 115-118 signal transducer and activator of transcription 3 Homo sapiens 109-114 23455323-6 2014 We demonstrate that activation of STAT3 serine-727 and tyrosine-705 phosphorylations is promoted by B-RAF(V600E) activity and that the Mcl-1 promoter is dependent on a STAT consensus-site for B-RAF-mediated activation. Serine 40-46 signal transducer and activator of transcription 3 Homo sapiens 34-39 23455323-6 2014 We demonstrate that activation of STAT3 serine-727 and tyrosine-705 phosphorylations is promoted by B-RAF(V600E) activity and that the Mcl-1 promoter is dependent on a STAT consensus-site for B-RAF-mediated activation. Serine 40-46 signal transducer and activator of transcription 3 Homo sapiens 34-38 23872421-6 2013 Furthermore, serine but not tyrosine dominant negative mutant of STAT3 impaired NRTN induced neurite outgrowth, indicative of the role of STAT3 as a downstream mediator of NRTN function. Serine 13-19 signal transducer and activator of transcription 3 Homo sapiens 138-143 24312439-0 2013 The MEK-ERK pathway is necessary for serine phosphorylation of mitochondrial STAT3 and Ras-mediated transformation. Serine 37-43 signal transducer and activator of transcription 3 Homo sapiens 77-82 24312439-4 2013 This mitochondrial activity of STAT3 was supported by phosphorylation on serine 727 (S727) in the carboxyl-terminus of STAT3. Serine 73-79 signal transducer and activator of transcription 3 Homo sapiens 31-36 24312439-4 2013 This mitochondrial activity of STAT3 was supported by phosphorylation on serine 727 (S727) in the carboxyl-terminus of STAT3. Serine 73-79 signal transducer and activator of transcription 3 Homo sapiens 119-124 24101906-6 2013 NSCs use an alternative phosphorylation site, STAT3-Ser, to regulate survival and growth, a site that is largely redundant for this function in most other cell types. Serine 52-55 signal transducer and activator of transcription 3 Homo sapiens 46-51 24055291-4 2013 Interestingly, we observed a decrease in expression of proteins that control the tumor micro environment such as VEGF-A, STAT-3, ERK1/2, ERK-p, AKT-1 ser 473 phosphorylation in compounds treated breast cancer cells. Serine 20-23 signal transducer and activator of transcription 3 Homo sapiens 121-127 24101906-7 2013 STAT3-Ser regulates Hes3, and together they form a convergence point for several signals, including Notch, Tie2, and insulin receptor activation. Serine 6-9 signal transducer and activator of transcription 3 Homo sapiens 0-5 24101906-8 2013 Disregulation and manipulation of the STAT3-Ser/Hes3 signaling pathway is important in both tumorigenesis and regenerative medicine, and worthy of extensive study. Serine 44-47 signal transducer and activator of transcription 3 Homo sapiens 38-43 23831387-3 2013 We show that NGF stimulates serine phosphorylation (S727) of STAT3 in sympathetic neurons via ERK1/2, in contrast to cytokine phosphorylation of Y705. Serine 28-34 signal transducer and activator of transcription 3 Homo sapiens 61-66 23329839-5 2013 We further show that Gadd45a deletion increases phosphorylation of STAT3 at Ser-727 and, in turn, elevates the STAT3 transcriptional activity. Serine 76-79 signal transducer and activator of transcription 3 Homo sapiens 67-72 23740979-0 2013 Kaposi"s sarcoma-associated herpesvirus kaposin B induces unique monophosphorylation of STAT3 at serine 727 and MK2-mediated inactivation of the STAT3 transcriptional repressor TRIM28. Serine 97-103 signal transducer and activator of transcription 3 Homo sapiens 88-93 23329839-5 2013 We further show that Gadd45a deletion increases phosphorylation of STAT3 at Ser-727 and, in turn, elevates the STAT3 transcriptional activity. Serine 76-79 signal transducer and activator of transcription 3 Homo sapiens 111-116 23329839-7 2013 Interestingly, Gadd45a is able to physically associate with mammalian target of rapamycin (mTOR), a kinase that mediates Ser-727 phosphorylation of STAT3. Serine 121-124 signal transducer and activator of transcription 3 Homo sapiens 148-153 23329839-8 2013 The interaction of Gadd45a with mTOR suppresses STAT3 phosphorylation at Ser-727 and leads to down-regulated expression of VEGFa. Serine 73-76 signal transducer and activator of transcription 3 Homo sapiens 48-53 23174178-9 2013 In some cases, p-STAT3 at Ser(727) could also be detected, while p-STAT1 at Ser(727) could not. Serine 26-29 signal transducer and activator of transcription 3 Homo sapiens 17-22 23125416-6 2012 Subcellular fractionation and confocal microscopy illustrated that Tyr(705)-phosphorylated STAT3 translocated to the nucleus, whereas Ser(727)-phosphorylated STAT3 was retained in the cytosol after CCR1/Galpha(14) activation. Serine 134-137 signal transducer and activator of transcription 3 Homo sapiens 158-163 22967106-7 2012 Similarly, using a mutated form of p27, where the cysteine 211-isoprenylation residue was replaced by a serine, a significant reduction of STAT-3 and NF-kappaB activation was seen, suggesting the involvement of isoprenylation in this process. Serine 104-110 signal transducer and activator of transcription 3 Homo sapiens 139-145 22922995-8 2012 The change in the serine phosphorylation of STAT3 was subtle during the examination periods. Serine 18-24 signal transducer and activator of transcription 3 Homo sapiens 56-61 22696236-3 2012 As(3+) activates JNK and induces phosphorylation of the Stat3 at serine 727 (S727) in a dose- and time-dependent manner, which occurred concomitantly with Akt activation. Serine 65-71 signal transducer and activator of transcription 3 Homo sapiens 56-61 21954831-2 2012 In the present study, we show that this functional coupling elicited biphasic stimulatory phosphorylation on STAT3 in recombinant MT(1) /Galpha(16) cells and native Jurkat T cells (endogenously expressing MT(1) and Galpha(16) ), with maximal Ser(727) phosphorylation occurring at 15min, while marked Tyr(705) phosphorylation became detectable only upon agonist treatment for 4 hr or more. Serine 242-245 signal transducer and activator of transcription 3 Homo sapiens 109-114 22335604-5 2012 Employing western blot analysis, we found that exposure of NHEK to UVB, but not UVA, phosphorylates JNK1/2 at Th(183)/Tyr(185), STAT3 at Ser(727) , AKT at Ser(473) and increases c-Fos expression, whereas exposure to UVA, but not UVB, phosphorylates AKT at Thr(308). Serine 137-140 signal transducer and activator of transcription 3 Homo sapiens 128-133 22393233-5 2012 In the study reported here we demonstrate that during TNF-induced necroptosis, STAT3 is phosphorylated on serine 727, which is dependent on RIPK-1 expression or activity. Serine 106-112 signal transducer and activator of transcription 3 Homo sapiens 79-84 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Serine 208-211 signal transducer and activator of transcription 3 Homo sapiens 13-63 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Serine 208-211 signal transducer and activator of transcription 3 Homo sapiens 65-70 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Serine 208-211 signal transducer and activator of transcription 3 Homo sapiens 107-112 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Serine 208-211 signal transducer and activator of transcription 3 Homo sapiens 107-112 21954831-3 2012 By employing signal transducer and activator of transcription 3 (STAT3) phosphorylation-resistant mutants (STAT3-Y705F and STAT3-S727A), we further showed that the receptor-mediated STAT3 phosphorylations at Ser(727) and Tyr(705) were independent of each other. Serine 208-211 signal transducer and activator of transcription 3 Homo sapiens 107-112 23275693-5 2012 The involvement of residues like LYS-591, ARG-609, SER-611, GLU-612, SER-613, SER-636 and VAL-637 seems to play an important role in binding of curcumin natural derivatives and its amino acids conjugates with Src Homology (SH2) domain of Stat3 monomer. Serine 51-54 signal transducer and activator of transcription 3 Homo sapiens 238-243 23275693-5 2012 The involvement of residues like LYS-591, ARG-609, SER-611, GLU-612, SER-613, SER-636 and VAL-637 seems to play an important role in binding of curcumin natural derivatives and its amino acids conjugates with Src Homology (SH2) domain of Stat3 monomer. Serine 69-72 signal transducer and activator of transcription 3 Homo sapiens 238-243 23275693-5 2012 The involvement of residues like LYS-591, ARG-609, SER-611, GLU-612, SER-613, SER-636 and VAL-637 seems to play an important role in binding of curcumin natural derivatives and its amino acids conjugates with Src Homology (SH2) domain of Stat3 monomer. Serine 69-72 signal transducer and activator of transcription 3 Homo sapiens 238-243 21878628-6 2011 Further, we found that Src tyrosine kinase-mediated activation of p38 MAPK is required for Stat1 and Stat3 serine 727 phosphorylation in alternatively activated monocytes/macrophages. Serine 107-113 signal transducer and activator of transcription 3 Homo sapiens 101-106 23226414-3 2012 As well, OSM and IL-6/R induce tyrosine and serine phosphorylation of STAT3 in primary cortical neurons and SH-SY5Y cells. Serine 44-50 signal transducer and activator of transcription 3 Homo sapiens 70-75 22174695-0 2011 The FGFR4-G388R polymorphism promotes mitochondrial STAT3 serine phosphorylation to facilitate pituitary growth hormone cell tumorigenesis. Serine 58-64 signal transducer and activator of transcription 3 Homo sapiens 52-57 22174695-4 2011 Growth promoting effects of FGFR4-R388 as evidenced by enhanced colony formation was ascribed to Src activation and mitochondrial serine phosphorylation of STAT3 (pS-STAT3). Serine 130-136 signal transducer and activator of transcription 3 Homo sapiens 156-161 21325634-7 2011 Second, curcumin suppressed activation of signal transducer and activator of transcription-3 as indicated by decreased phosphorylation at both tyrosine(705) and serine(727) and inhibition of janus kinase-1 phosphorylation. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 42-92 21810609-4 2011 Of importance, we found negative regulatory effects of KAP1 on the serine phosphorylation of STAT3, the acetylation of NF-kappaB/p65 by p300, and the nuclear localization of NF-kappaB/p65. Serine 67-73 signal transducer and activator of transcription 3 Homo sapiens 93-98 21619877-9 2011 Finally, endocytic trafficking played a central role in regulating STAT3 serine 727 phosphorylation through crosstalk with the MAPK signaling system. Serine 73-79 signal transducer and activator of transcription 3 Homo sapiens 67-72 21490133-4 2011 STAT3 phosphorylation at both tyrosine residue 705 and serine residue 727 was inhibited by GA. Serine 55-61 signal transducer and activator of transcription 3 Homo sapiens 0-5 21801384-13 2011 Finally, HAK compounds were demonstrated to specifically suppress the OSM-induced phosphorylation of STAT3 at serine 727 and the physical interaction of pSTAT3S727 with p65. Serine 110-116 signal transducer and activator of transcription 3 Homo sapiens 101-106 20979115-7 2011 RESULTS: HDL increased serine 727 phosphorylation of Stat3, but not tyrosine 705 only in DU145 cells. Serine 23-29 signal transducer and activator of transcription 3 Homo sapiens 53-58 21107604-5 2011 Genetic study revealed a heterozygous mutation in exon 21 of STAT3 gene (g.66583 A > C, c.1970A > C) in the boy, which resulted in a substitution of tyrosine at the amino acid position 657 to serine (p.Y657S) in the Src homology 2 (SH2) domain of STAT3. Serine 198-204 signal transducer and activator of transcription 3 Homo sapiens 61-66 21307189-6 2011 The kinase responsible for LMP1-CTAR1-mediated serine phosphorylation of STAT3 was identified to be PKCdelta using specific RNAi, a dominant negative PKCdelta, and the PKCdelta inhibitor rottlerin. Serine 47-53 signal transducer and activator of transcription 3 Homo sapiens 73-78 20473906-6 2011 We demonstrate that the intracellular signal transducer and activator of transcription STAT3 is constitutively activated by phosphorylation preferentially on serine 727 in these cells. Serine 158-164 signal transducer and activator of transcription 3 Homo sapiens 87-92 21398617-4 2011 STAT3 is activated via phosphorylation of serine 727 but also NFAT2 through its translocation into the nucleus. Serine 42-48 signal transducer and activator of transcription 3 Homo sapiens 0-5 20978825-3 2011 In response to IL-6, the signal transducer and activator of transcription 3 (STAT3) becomes phosphorylated on tyrosine and serine residues. Serine 123-129 signal transducer and activator of transcription 3 Homo sapiens 25-75 20978825-3 2011 In response to IL-6, the signal transducer and activator of transcription 3 (STAT3) becomes phosphorylated on tyrosine and serine residues. Serine 123-129 signal transducer and activator of transcription 3 Homo sapiens 77-82 20978825-9 2011 Preincubating HepG(2) cells with adiponectin led to a decline in STAT3 phosphorylation on both tyrosine and serine residues. Serine 108-114 signal transducer and activator of transcription 3 Homo sapiens 65-70 20691278-6 2010 Specifically, we reveal that JAK2 mediates STAT3 tyrosine phosphorylation in response to the two BMPs, whereas BMP2- and BMP4-induced STAT3 serine phosphorylation involves two divergent cascades, namely the mTOR and ERK1/2 cascades, respectively. Serine 140-146 signal transducer and activator of transcription 3 Homo sapiens 134-139 22870139-5 2011 Notably, the expression level of signal transducers and activators of transcription 3 (STAT3) and serine 727-phosphorylated STAT3 (pSTAT3-Ser727) was highly increased in TR cells, while that of 705-phosphorylated STAT3 (pSTAT3-Tyr705) was decreased. Serine 98-104 signal transducer and activator of transcription 3 Homo sapiens 124-129 22870139-5 2011 Notably, the expression level of signal transducers and activators of transcription 3 (STAT3) and serine 727-phosphorylated STAT3 (pSTAT3-Ser727) was highly increased in TR cells, while that of 705-phosphorylated STAT3 (pSTAT3-Tyr705) was decreased. Serine 98-104 signal transducer and activator of transcription 3 Homo sapiens 124-129 21159613-1 2010 Phosphorylation of STAT3 on serine 727 regulates gene expression and is found to be elevated in many B-leukemia cells including chronic lymphocytic leukemia (CLL). Serine 28-34 signal transducer and activator of transcription 3 Homo sapiens 19-24 21159613-10 2010 Thus, we present here for the first time that JSI-124 induced suppression of serine 727 phosphorylation of STAT3, leading to apoptosis and cell-cycle arrest through alterations in gene transcription in B-leukemia cells. Serine 77-83 signal transducer and activator of transcription 3 Homo sapiens 107-112 20361349-11 2010 Only phosphorylation of STAT3 at ser 727 site paralleled the IL-6 stimulation, and JSI-124, a specific JAK-STAT3 pathway blocker, deterred the invasion and migration promotive effect of IL-6, indicating that the JAK/STAT3 pathway mediates signal transduction. Serine 33-36 signal transducer and activator of transcription 3 Homo sapiens 24-29 19009233-6 2009 In addition, trichodimerol blocked IFN-gamma, IL-6 and IL-4 induced activation of Stat1, Stat3 and Stat6 transcription factors by inhibiting serine and tyrosine phosphorylation. Serine 141-147 signal transducer and activator of transcription 3 Homo sapiens 89-94 20516069-5 2010 Following treatment, STAT3 is phosphorylated on its C-terminal serine 727 residue but not on its tyrosine 705 site. Serine 63-69 signal transducer and activator of transcription 3 Homo sapiens 21-26 20516069-9 2010 ChIP experiments indicated that STAT3 can be found associated with the Eme1 promoter when phosphorylated only on its serine 727 residue and not on tyrosine 705. Serine 117-123 signal transducer and activator of transcription 3 Homo sapiens 32-37 20154216-0 2010 STAT3 is constitutively phosphorylated on serine 727 residues, binds DNA, and activates transcription in CLL cells. Serine 42-48 signal transducer and activator of transcription 3 Homo sapiens 0-5 20154216-3 2010 In CLL, however, STAT3 is constitutively phosphorylated on serine 727, not tyrosine 705, residues. Serine 59-65 signal transducer and activator of transcription 3 Homo sapiens 17-22 20154216-7 2010 Furthermore, infection of CLL cells with lentiviral STAT3-small hairpin RNA reduced the expression of several STAT3-regulated survival and proliferation genes and induced apoptosis, suggesting that constitutive serine pSTAT3 initiates transcription in CLL cells. Serine 211-217 signal transducer and activator of transcription 3 Homo sapiens 52-57 20154216-7 2010 Furthermore, infection of CLL cells with lentiviral STAT3-small hairpin RNA reduced the expression of several STAT3-regulated survival and proliferation genes and induced apoptosis, suggesting that constitutive serine pSTAT3 initiates transcription in CLL cells. Serine 211-217 signal transducer and activator of transcription 3 Homo sapiens 110-115 20154216-8 2010 Taken together, our data suggest that constitutive phosphorylation of STAT3 on serine 727 residues is a hallmark of CLL and that STAT3 be considered a therapeutic target in this disease. Serine 79-85 signal transducer and activator of transcription 3 Homo sapiens 70-75 19875458-0 2009 Signal transducer and activator of transcription 3 (STAT3) mediates amino acid inhibition of insulin signaling through serine 727 phosphorylation. Serine 119-125 signal transducer and activator of transcription 3 Homo sapiens 0-50 19875458-0 2009 Signal transducer and activator of transcription 3 (STAT3) mediates amino acid inhibition of insulin signaling through serine 727 phosphorylation. Serine 119-125 signal transducer and activator of transcription 3 Homo sapiens 52-57 19875458-7 2009 Amino acids stimulated the phosphorylation of STAT3 at Ser(727), but not Tyr(705). Serine 55-58 signal transducer and activator of transcription 3 Homo sapiens 46-51 19875458-8 2009 Replacement of the endogenous STAT3 with wild-type, but not S727A, recombinant STAT3 restored the ability of amino acids to inhibit insulin signaling, suggesting that Ser(727) phosphorylation was critical for STAT3-mediated amino acid effect. Serine 167-170 signal transducer and activator of transcription 3 Homo sapiens 30-35 19875458-10 2009 Our results also indicated that mammalian target of rapamycin was likely responsible for the phosphorylation of STAT3 at Ser(727) in response to excess amino acids. Serine 121-124 signal transducer and activator of transcription 3 Homo sapiens 112-117 19734018-10 2009 Histamine enhanced STAT3 transcriptional activity and induced tyrosine and serine phosphorylation of STAT3. Serine 75-81 signal transducer and activator of transcription 3 Homo sapiens 101-106 19429262-4 2009 Serine phosphorylation of STAT3 was observed as a result of cadmium treatment while no tyrosine phosphorylation was detected. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-31 19797267-3 2009 STAT3 modifed by serine phosphorylation augmented oxidative phosphorylation in mitochondria and supported cellular transformation by oncogenic Ras. Serine 17-23 signal transducer and activator of transcription 3 Homo sapiens 0-5 19723038-7 2009 The phosphorylation of STAT-3 at serine 727 may regulate its activity negatively or positively. Serine 33-39 signal transducer and activator of transcription 3 Homo sapiens 23-29 19764561-3 2009 RESULTS: (1) The expression of p-ERK was activated at 1 h and remained at a high level until 4 h. (2) The expression of p-STAT3 (Ser) was activated at 30 min and remained at a high level until 4 h. CONCLUSION: Tensile stress can activate the expression of p-ERK and p-STAT3 (Ser) in alveolar epithelium A549 cells. Serine 129-132 signal transducer and activator of transcription 3 Homo sapiens 122-127 19764561-3 2009 RESULTS: (1) The expression of p-ERK was activated at 1 h and remained at a high level until 4 h. (2) The expression of p-STAT3 (Ser) was activated at 30 min and remained at a high level until 4 h. CONCLUSION: Tensile stress can activate the expression of p-ERK and p-STAT3 (Ser) in alveolar epithelium A549 cells. Serine 129-132 signal transducer and activator of transcription 3 Homo sapiens 268-273 19764561-3 2009 RESULTS: (1) The expression of p-ERK was activated at 1 h and remained at a high level until 4 h. (2) The expression of p-STAT3 (Ser) was activated at 30 min and remained at a high level until 4 h. CONCLUSION: Tensile stress can activate the expression of p-ERK and p-STAT3 (Ser) in alveolar epithelium A549 cells. Serine 275-278 signal transducer and activator of transcription 3 Homo sapiens 122-127 19429262-11 2009 Data suggest that STAT3 is phosphorylated at serine 727 by a Cd stress-activated signaling pathway inducing NADPH oxidase activity which produced ROS, leading ERK activation. Serine 45-51 signal transducer and activator of transcription 3 Homo sapiens 18-23 19885032-0 2009 Extracellular Signal-regulated Kinase Activation Is Required for Serine 727 Phosphorylation of STAT3 in Schwann Cells in vitro and in vivo. Serine 65-71 signal transducer and activator of transcription 3 Homo sapiens 95-100 19331815-0 2009 mTOR mediates human trophoblast invasion through regulation of matrix-remodeling enzymes and is associated with serine phosphorylation of STAT3. Serine 112-118 signal transducer and activator of transcription 3 Homo sapiens 138-143 19885032-2 2009 In this study, we determined that nerve injury-induced ERK activation was temporally correlated with STAT3 phosphorylation at the serine 727 residue. Serine 130-136 signal transducer and activator of transcription 3 Homo sapiens 101-106 19885032-6 2009 In contrast with the IL-6 response, serine phosphorylated STAT3 induced by NRG was not detected in the nucleus, thus indicating the non-nuclear function of serine phosphorylated STAT3 in response to NRG. Serine 156-162 signal transducer and activator of transcription 3 Homo sapiens 178-183 19885032-7 2009 Finally, we determined that the inhibition of ERK prevented injury-induced serine phosphorylation of STAT3 in an ex-vivo explants culture of the sciatic nerves. Serine 75-81 signal transducer and activator of transcription 3 Homo sapiens 101-106 19885032-8 2009 Collectively, the results of this study show that ERK may be an upstream kinase for the serine phosphorylation of STAT3 induced by multiple stimuli in Schwann cells after peripheral nerve injury. Serine 88-94 signal transducer and activator of transcription 3 Homo sapiens 114-119 19885032-3 2009 In cultured Schwann cells, we noted that ERK activation is required for the serine phosphorylation of STAT3 by neuropoietic cytokine interleukin-6 (IL-6). Serine 76-82 signal transducer and activator of transcription 3 Homo sapiens 102-107 19885032-4 2009 Serine phosphorylated STAT3 by IL-6 was transported into Schwann cell nuclei, thereby indicating that ERK may regulate the transcriptional activity of STAT3 via the induction of serine phosphorylation of STAT3. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 22-27 19885032-4 2009 Serine phosphorylated STAT3 by IL-6 was transported into Schwann cell nuclei, thereby indicating that ERK may regulate the transcriptional activity of STAT3 via the induction of serine phosphorylation of STAT3. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 151-156 19885032-4 2009 Serine phosphorylated STAT3 by IL-6 was transported into Schwann cell nuclei, thereby indicating that ERK may regulate the transcriptional activity of STAT3 via the induction of serine phosphorylation of STAT3. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 151-156 19885032-4 2009 Serine phosphorylated STAT3 by IL-6 was transported into Schwann cell nuclei, thereby indicating that ERK may regulate the transcriptional activity of STAT3 via the induction of serine phosphorylation of STAT3. Serine 178-184 signal transducer and activator of transcription 3 Homo sapiens 22-27 19885032-4 2009 Serine phosphorylated STAT3 by IL-6 was transported into Schwann cell nuclei, thereby indicating that ERK may regulate the transcriptional activity of STAT3 via the induction of serine phosphorylation of STAT3. Serine 178-184 signal transducer and activator of transcription 3 Homo sapiens 151-156 19885032-4 2009 Serine phosphorylated STAT3 by IL-6 was transported into Schwann cell nuclei, thereby indicating that ERK may regulate the transcriptional activity of STAT3 via the induction of serine phosphorylation of STAT3. Serine 178-184 signal transducer and activator of transcription 3 Homo sapiens 151-156 19885032-5 2009 Neuregulin-1 (NRG) also induced the serine phosphorylation of STAT3 in an ERK-dependent fashion. Serine 36-42 signal transducer and activator of transcription 3 Homo sapiens 62-67 19885032-6 2009 In contrast with the IL-6 response, serine phosphorylated STAT3 induced by NRG was not detected in the nucleus, thus indicating the non-nuclear function of serine phosphorylated STAT3 in response to NRG. Serine 36-42 signal transducer and activator of transcription 3 Homo sapiens 58-63 19121623-0 2009 HDAC3 influences phosphorylation of STAT3 at serine 727 by interacting with PP2A. Serine 45-51 signal transducer and activator of transcription 3 Homo sapiens 36-41 19097071-4 2009 For 18% of the cell lines, OSM resistance is associated with a phosphorylation defect of signal transducer and activator of transcription (STAT)3 on serine (Ser)727, in concordance with defects in the activation of various protein kinase C (PKC) isoforms, especially PKCdelta. Serine 149-155 signal transducer and activator of transcription 3 Homo sapiens 89-145 19097071-4 2009 For 18% of the cell lines, OSM resistance is associated with a phosphorylation defect of signal transducer and activator of transcription (STAT)3 on serine (Ser)727, in concordance with defects in the activation of various protein kinase C (PKC) isoforms, especially PKCdelta. Serine 157-160 signal transducer and activator of transcription 3 Homo sapiens 89-145 19351839-8 2009 Suppression of Ser(727) and Tyr(705) phosphorylation of STAT3 by KU-55933 reduced STAT3 transacting activity accompanied by elevation in DR5 expression. Serine 15-18 signal transducer and activator of transcription 3 Homo sapiens 56-61 19351839-8 2009 Suppression of Ser(727) and Tyr(705) phosphorylation of STAT3 by KU-55933 reduced STAT3 transacting activity accompanied by elevation in DR5 expression. Serine 15-18 signal transducer and activator of transcription 3 Homo sapiens 82-87 19182994-3 2009 Further studies show that both serine-727 and tyrosine-705 of Stat3 were phosphorylated while Ha-ras was overexpressed. Serine 31-37 signal transducer and activator of transcription 3 Homo sapiens 62-67 19182994-6 2009 We demonstrate that Ha-ras and Stat3 acting together synergistically induce Stat3 phosphorylation at serine-727 phosphorylation and cyclin D1 expression and further enhance transformation and tumorigenicity of the cell. Serine 101-107 signal transducer and activator of transcription 3 Homo sapiens 31-36 19182994-6 2009 We demonstrate that Ha-ras and Stat3 acting together synergistically induce Stat3 phosphorylation at serine-727 phosphorylation and cyclin D1 expression and further enhance transformation and tumorigenicity of the cell. Serine 101-107 signal transducer and activator of transcription 3 Homo sapiens 76-81 19182994-7 2009 Ha-ras-induced Stat3 phosphorylation at serine-727 plays a pivotal role in transcriptional activation of cyclin D1 and suppression of cell apoptosis. Serine 40-46 signal transducer and activator of transcription 3 Homo sapiens 15-20 19182994-8 2009 The effect of Ha-ras on Stat3 phosphorylation at serine-727 was also detected in human bladder (T24) and lung (H460) cancer cells. Serine 49-55 signal transducer and activator of transcription 3 Homo sapiens 24-29 19182994-9 2009 Stat3 phosphorylation at serine-727 is important in Ras-related tumorigenesis. Serine 25-31 signal transducer and activator of transcription 3 Homo sapiens 0-5 18398416-7 2008 The results indicated serine phosphorylation of STAT3 after PC or varepsilonPKC activation. Serine 22-28 signal transducer and activator of transcription 3 Homo sapiens 48-53 21479505-2 2008 Physiological STAT3 phosphorylation involves tyrosine (Y705) and serine (S727) activation. Serine 65-71 signal transducer and activator of transcription 3 Homo sapiens 14-19 18556347-9 2008 IL-1beta induced serine phosphorylation of inhibitory kappaBalpha, STAT1, and STAT3. Serine 17-23 signal transducer and activator of transcription 3 Homo sapiens 78-83 18676737-8 2008 RESULTS AND CONCLUSIONS: STAT-3 activation, as assessed by tyrosine and serine phosphorylation, was elevated in GBM tissue compared with control tissue. Serine 72-78 signal transducer and activator of transcription 3 Homo sapiens 25-31 18752497-2 2008 METHODS AND RESULTS: By immunohistochemistry, both cytoplasmic and nuclear expression of p-Stat3-Ser(727) was demonstrated in 88 trophoblastic tissues, including placentas and GTD. Serine 97-100 signal transducer and activator of transcription 3 Homo sapiens 91-96 18752497-3 2008 Nuclear immunoreactivity of p-Stat3-Ser(727) was significantly higher in hydatidiform mole (HM) (P < 0.001) and choriocarcinoma (P = 0.009) when compared with normal placentas. Serine 36-39 signal transducer and activator of transcription 3 Homo sapiens 30-35 18752497-4 2008 Placental site trophoblastic tumours (PSTT) and epithelioid trophoblastic tumours (ETT) also demonstrated higher nuclear p-Stat3-Ser(727) expression than their normal trophoblast counterparts. Serine 129-132 signal transducer and activator of transcription 3 Homo sapiens 123-128 18752497-5 2008 Higher p-Stat3-Ser(727) expression was confirmed in choriocarcinoma cell lines, JEG-3 and JAR, than in a normal trophoblast cell line, with both nuclear and cytoplasmic fractions demonstrated by immunoblotting. Serine 15-18 signal transducer and activator of transcription 3 Homo sapiens 9-14 18752497-6 2008 Spontaneously regressed HM showed significantly increased nuclear and cytoplasmic p-Stat3-Ser(727) immunoreactivity over those that developed gestational trophoblastic neoplasia (GTN) (P = 0.013, P = 0.039). Serine 90-93 signal transducer and activator of transcription 3 Homo sapiens 84-89 18752497-7 2008 There was a significant positive and inverse correlation between nuclear p-Stat3-Ser(727) immunoreactivity and apoptotic indices [terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling and M30 CytoDeath antibody] (P = 0.001, P < 0.001, Spearman"s rho test) and Bcl-2 expression (P = 0.034), respectively. Serine 81-84 signal transducer and activator of transcription 3 Homo sapiens 75-80 18752497-8 2008 CONCLUSIONS: p-Stat3-Ser(727) plays a role in the pathogenesis of GTD, probably through the regulation of apoptosis. Serine 21-24 signal transducer and activator of transcription 3 Homo sapiens 15-20 18752497-9 2008 p-Stat3-Ser(727) immunoreactivity is a potential marker in predicting GTN in HM. Serine 8-11 signal transducer and activator of transcription 3 Homo sapiens 2-7 18829527-0 2008 Activation of signal transducer and activator of transcription 3 through a phosphomimetic serine 727 promotes prostate tumorigenesis independent of tyrosine 705 phosphorylation. Serine 90-96 signal transducer and activator of transcription 3 Homo sapiens 14-64 18550668-5 2008 B19 infection as well as NS1 overexpression in HMEC-1 cells produced a significant upregulation in the phosphorylation of both tyrosine(705) and serine(727) STAT3 (P < 0.05). Serine 145-151 signal transducer and activator of transcription 3 Homo sapiens 157-162 18499896-6 2008 This was partially attributed to Hoxb1-dependent activation of the Notch signaling pathway and Notch-dependent STAT3 phosphorylation at Ser 727, thus linking Hox gene function with maintenance of active Notch signaling and the JAK/STAT pathway. Serine 136-139 signal transducer and activator of transcription 3 Homo sapiens 111-116 18398416-8 2008 Inhibition of either varepsilonPKC or ERK1/2 activation abolished PC-induced serine phosphorylation of STAT3. Serine 77-83 signal transducer and activator of transcription 3 Homo sapiens 103-108 17703129-6 2007 Moreover, we hypothesize that hyperactivation of the JAK kinases, particularly JAK2, mismatched STAT3 serine-tyrosine phosphorylation or heightened STAT3 transcriptional activity, and SOCS3 induction may ultimately prove detrimental. Serine 102-108 signal transducer and activator of transcription 3 Homo sapiens 96-101 18204781-3 2008 LMP1 stimulated STAT3 Tyr 705-dependent nuclear accumulation, as well as the phosphorylation of STAT3 at both Tyr 705 and Ser 727. Serine 122-125 signal transducer and activator of transcription 3 Homo sapiens 96-101 18204781-7 2008 Inhibition of ERK activity by an inhibitor (PD98059) of MAPK/extracellular signal-regulated kinase kinase (MEK1) decreased the LMP1-induced activation of STAT3 Ser 727. Serine 160-163 signal transducer and activator of transcription 3 Homo sapiens 154-159 17993646-6 2008 Interestingly, we find that the phosphorylation of STAT3 on Ser(727) and STAT3 transcriptional activity are regulated by mTOR upon IL-6 stimulation and that STAT3 is required for IL-6 inhibition of insulin signaling. Serine 60-63 signal transducer and activator of transcription 3 Homo sapiens 51-56 18583177-5 2008 Janus-activated kinase 2 was required for signal transducer and activator of transcription 3 tyrosine phosphorylation, whereas the extracellular signal-regulated kinase 1/2 mediated signal transducer and activator of transcription 3 serine phosphorylation in response to platelet-derived growth factor-BB. Serine 233-239 signal transducer and activator of transcription 3 Homo sapiens 182-232 17636039-5 2007 PGE2 stimulation resulted in STAT3 serine and tyrosine phosphorylation, although mutation of either of the two previously characterized STAT response elements on the SOCS3 promoter did not affect SOCS3 promoter activation by PGE2. Serine 35-41 signal transducer and activator of transcription 3 Homo sapiens 29-34 18519681-5 2008 The decrease in STAT3(Tyr705) phosphorylation was linked to a TGFbeta/Smad4-dependent and enhanced activation of extracellular signal-regulated kinases, which caused an increase in serine phosphorylation of STAT3(Ser727). Serine 181-187 signal transducer and activator of transcription 3 Homo sapiens 16-21 18519681-5 2008 The decrease in STAT3(Tyr705) phosphorylation was linked to a TGFbeta/Smad4-dependent and enhanced activation of extracellular signal-regulated kinases, which caused an increase in serine phosphorylation of STAT3(Ser727). Serine 181-187 signal transducer and activator of transcription 3 Homo sapiens 207-212 17917251-1 2007 Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine or serine phosphorylation, dimerization and nuclear translocation. Serine 197-203 signal transducer and activator of transcription 3 Homo sapiens 0-50 17917251-1 2007 Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine or serine phosphorylation, dimerization and nuclear translocation. Serine 197-203 signal transducer and activator of transcription 3 Homo sapiens 52-57 17267401-3 2007 Insulin reduces the tyrosine phosphorylation and increases the serine phosphorylation of STAT3, thereby reducing its nuclear localization and transcriptional activity. Serine 63-69 signal transducer and activator of transcription 3 Homo sapiens 89-94 17543278-4 2007 LIGHT induces dose-dependent activation of Stat3 by phosphorylation at both the tyrosine 705 and serine 727 residues. Serine 97-103 signal transducer and activator of transcription 3 Homo sapiens 43-48 17543278-7 2007 Overexpression of an active NIK induces Stat3 activation by phosphorylation at the both tyrosine 705 and serine 727 residues. Serine 105-111 signal transducer and activator of transcription 3 Homo sapiens 40-45 17496330-4 2007 Remarkably, by virtue of mammalian target of rapamycin/STAT3 Pi-Ser-727, Eps8 modulates FAK expression required for cell proliferation. Serine 64-67 signal transducer and activator of transcription 3 Homo sapiens 55-60 17145757-9 2007 Significantly, the STAT3 pathway was involved in Cdk5-dependent proliferation of MTC cells through Ser-727 phosphorylation. Serine 99-102 signal transducer and activator of transcription 3 Homo sapiens 19-24 17209045-1 2007 The precise role of STAT3 Ser(727) phosphorylation in RET-mediated cell transformation and oncogenesis is not well understood. Serine 26-29 signal transducer and activator of transcription 3 Homo sapiens 20-25 17209045-3 2007 Using inhibitors and dominant negative constructs, we have demonstrated that RET(Y791F) and RET(S891A) induce STAT3 Ser(727) phosphorylation via a canonical Ras/ERK1/2 pathway and that integration of the Ras/ERK1/2/ELK-1 and STAT3 pathways was required for up-regulation of the c-fos promoter by FMTC-RET. Serine 116-119 signal transducer and activator of transcription 3 Homo sapiens 110-115 17209045-3 2007 Using inhibitors and dominant negative constructs, we have demonstrated that RET(Y791F) and RET(S891A) induce STAT3 Ser(727) phosphorylation via a canonical Ras/ERK1/2 pathway and that integration of the Ras/ERK1/2/ELK-1 and STAT3 pathways was required for up-regulation of the c-fos promoter by FMTC-RET. Serine 116-119 signal transducer and activator of transcription 3 Homo sapiens 225-230 17209045-7 2007 These cells displayed elevated levels of activated ERK1/2 and Ser(727)-phosphorylated STAT3, which were inhibited by treatment with U0126. Serine 62-65 signal transducer and activator of transcription 3 Homo sapiens 86-91 17209045-9 2007 Immunohistochemistry in tumor samples from FMTC patients showed strong nuclear staining of phosphorylated ERK1/2 and Ser(727) STAT3. Serine 117-120 signal transducer and activator of transcription 3 Homo sapiens 126-131 17209045-10 2007 These data show that FMTC-RET mutants activate a Ras/ERK1/2/STAT3 Ser(727) pathway, which plays an important role in cell mitogenicity and transformation. Serine 66-69 signal transducer and activator of transcription 3 Homo sapiens 60-65 17027757-1 2006 To achieve maximal transcriptional activity in response to gp130 cytokines, Serine-727 (Ser-727) of Stat3 is phosphorylated. Serine 76-82 signal transducer and activator of transcription 3 Homo sapiens 100-105 17113289-4 2007 The order of affinity for Stat3 was Gln > Ser(CONH2) >> Thr(CONH2) suggesting a relatively tight binding pocket for the side chain of glutamine. Serine 45-48 signal transducer and activator of transcription 3 Homo sapiens 26-31 17258468-8 2006 Inhibition of tyrosine/serine phosphorylation mediated by MAPKs of STAT1 and STAT3 decrease the IL-6 secretion following porin stimulation. Serine 23-29 signal transducer and activator of transcription 3 Homo sapiens 77-82 17258468-9 2006 Therefore, suggesting a key role of this pathway in phosphorylation of Ser 727 in STAT1 and STAT3. Serine 71-74 signal transducer and activator of transcription 3 Homo sapiens 92-97 16879821-7 2006 Phosphorylation of STAT3 at Serine 727 by CDK5 decreased during serum deprivation, and partly recovered by mu-opioid agonist. Serine 28-34 signal transducer and activator of transcription 3 Homo sapiens 19-24 17027757-1 2006 To achieve maximal transcriptional activity in response to gp130 cytokines, Serine-727 (Ser-727) of Stat3 is phosphorylated. Serine 76-79 signal transducer and activator of transcription 3 Homo sapiens 100-105 17027757-2 2006 Ser-727 resides in the LPMSP motif, the only conserved sequence among the transcription activation domains of several STATs. Serine 0-3 signal transducer and activator of transcription 3 Homo sapiens 118-123 17027757-3 2006 We show here that in addition to Ser-727, other residues in this LPMSP motif are also required for Stat3 activity in response to cytokine signaling through regulation of Ser-727 phosphorylation and recruitment of the transcription co-activator CBP/p300 to the promoters of Stat3-target genes for transcription activation. Serine 33-36 signal transducer and activator of transcription 3 Homo sapiens 99-104 17027757-3 2006 We show here that in addition to Ser-727, other residues in this LPMSP motif are also required for Stat3 activity in response to cytokine signaling through regulation of Ser-727 phosphorylation and recruitment of the transcription co-activator CBP/p300 to the promoters of Stat3-target genes for transcription activation. Serine 170-173 signal transducer and activator of transcription 3 Homo sapiens 99-104 16540667-6 2006 In non-small-cell lung cancer cells, STAT3 activity is regulated by EGFR through modulation of STAT3 serine phosphorylation. Serine 101-107 signal transducer and activator of transcription 3 Homo sapiens 37-42 16951338-9 2006 Furthermore, by transfecting monocytes with Stat3 containing mutations in Tyr705 or Ser727 or with wild-type Stat3, we demonstrated that both Stat3 tyrosine and serine phosphorylations are required for optimal binding of Stat3 with DNA and maximal expression of 15-lipoxygenase, an important regulator of inflammation and apoptosis. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 44-49 16951338-9 2006 Furthermore, by transfecting monocytes with Stat3 containing mutations in Tyr705 or Ser727 or with wild-type Stat3, we demonstrated that both Stat3 tyrosine and serine phosphorylations are required for optimal binding of Stat3 with DNA and maximal expression of 15-lipoxygenase, an important regulator of inflammation and apoptosis. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 109-114 16951338-9 2006 Furthermore, by transfecting monocytes with Stat3 containing mutations in Tyr705 or Ser727 or with wild-type Stat3, we demonstrated that both Stat3 tyrosine and serine phosphorylations are required for optimal binding of Stat3 with DNA and maximal expression of 15-lipoxygenase, an important regulator of inflammation and apoptosis. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 109-114 16951338-9 2006 Furthermore, by transfecting monocytes with Stat3 containing mutations in Tyr705 or Ser727 or with wild-type Stat3, we demonstrated that both Stat3 tyrosine and serine phosphorylations are required for optimal binding of Stat3 with DNA and maximal expression of 15-lipoxygenase, an important regulator of inflammation and apoptosis. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 109-114 16669628-0 2006 Phosphorylation of STAT3 serine-727 by cyclin-dependent kinase 1 is critical for nocodazole-induced mitotic arrest. Serine 25-31 signal transducer and activator of transcription 3 Homo sapiens 19-24 16669628-2 2006 While phosphorylation of STAT3 at Tyr-705 has been demonstrated to be a prerequisite for STAT3 dimerization, nuclear translocation, and activation of gene transcription, the role of Ser-727 in regulation of STAT3 activity is controversial. Serine 182-185 signal transducer and activator of transcription 3 Homo sapiens 25-30 16669628-5 2006 The nocodazole-induced STAT3 Ser-727 phosphorylation was reduced by selective inhibition of CDK1 phosphotransferase activity, and CDK1 could directly phosphorylate GST-STAT3 Ser-727 in vitro and co-immunoprecipitate with STAT3 in vivo. Serine 29-32 signal transducer and activator of transcription 3 Homo sapiens 23-28 16669628-5 2006 The nocodazole-induced STAT3 Ser-727 phosphorylation was reduced by selective inhibition of CDK1 phosphotransferase activity, and CDK1 could directly phosphorylate GST-STAT3 Ser-727 in vitro and co-immunoprecipitate with STAT3 in vivo. Serine 29-32 signal transducer and activator of transcription 3 Homo sapiens 168-173 16669628-5 2006 The nocodazole-induced STAT3 Ser-727 phosphorylation was reduced by selective inhibition of CDK1 phosphotransferase activity, and CDK1 could directly phosphorylate GST-STAT3 Ser-727 in vitro and co-immunoprecipitate with STAT3 in vivo. Serine 29-32 signal transducer and activator of transcription 3 Homo sapiens 168-173 16669628-5 2006 The nocodazole-induced STAT3 Ser-727 phosphorylation was reduced by selective inhibition of CDK1 phosphotransferase activity, and CDK1 could directly phosphorylate GST-STAT3 Ser-727 in vitro and co-immunoprecipitate with STAT3 in vivo. Serine 174-177 signal transducer and activator of transcription 3 Homo sapiens 23-28 16669628-5 2006 The nocodazole-induced STAT3 Ser-727 phosphorylation was reduced by selective inhibition of CDK1 phosphotransferase activity, and CDK1 could directly phosphorylate GST-STAT3 Ser-727 in vitro and co-immunoprecipitate with STAT3 in vivo. Serine 174-177 signal transducer and activator of transcription 3 Homo sapiens 168-173 16669628-5 2006 The nocodazole-induced STAT3 Ser-727 phosphorylation was reduced by selective inhibition of CDK1 phosphotransferase activity, and CDK1 could directly phosphorylate GST-STAT3 Ser-727 in vitro and co-immunoprecipitate with STAT3 in vivo. Serine 174-177 signal transducer and activator of transcription 3 Homo sapiens 168-173 16669628-6 2006 Blocking Ser-727 phosphorylation enhanced STAT3 DNA-binding activity toward its target gene promoters, implying a negative effect of Ser-727 phosphorylation on its transcriptional activity. Serine 9-12 signal transducer and activator of transcription 3 Homo sapiens 42-47 16720575-6 2006 In an attempt to resolve the mechanism by which this was occurring, we examined the effect of heparin on STAT3 Ser-727 phosphorylation and extracellular signal-regulated kinases 1 and 2 (Erk1/2) activation, either in the presence or absence of IL-11. Serine 111-114 signal transducer and activator of transcription 3 Homo sapiens 105-110 16425286-1 2006 Although it is known that STAT3 transcriptional activity is modulated by phosphorylation at serine residue 727, the role of STAT3 serine phosphorylation in breast cancer remains mostly unexplored. Serine 92-98 signal transducer and activator of transcription 3 Homo sapiens 26-31 16425286-1 2006 Although it is known that STAT3 transcriptional activity is modulated by phosphorylation at serine residue 727, the role of STAT3 serine phosphorylation in breast cancer remains mostly unexplored. Serine 130-136 signal transducer and activator of transcription 3 Homo sapiens 124-129 16425286-2 2006 In this study, we examined the expression patterns of serine residue 727-phosphorylated STAT3 (p-ser727-STAT3) in breast infiltrating ductal carcinoma tissues and nearby noncancer tissues by using immunoblotting techniques, and correlated the expression profiles with clinicopathological characteristics. Serine 54-60 signal transducer and activator of transcription 3 Homo sapiens 88-93 16425286-2 2006 In this study, we examined the expression patterns of serine residue 727-phosphorylated STAT3 (p-ser727-STAT3) in breast infiltrating ductal carcinoma tissues and nearby noncancer tissues by using immunoblotting techniques, and correlated the expression profiles with clinicopathological characteristics. Serine 54-60 signal transducer and activator of transcription 3 Homo sapiens 95-109 16567810-8 2006 The present study also implicates that extracellular signal-regulated kinase (ERK1/2), which are serine/threonine kinases, are the mediators of STAT3 serine phosphorylation upon AVP stimulation. Serine 97-103 signal transducer and activator of transcription 3 Homo sapiens 144-149 16567810-9 2006 We further suggest that AVP-induced STAT3 serine phosphorylation negatively modulates AVP-induced STAT3 tyrosine phosphorylation. Serine 42-48 signal transducer and activator of transcription 3 Homo sapiens 36-41 16567810-9 2006 We further suggest that AVP-induced STAT3 serine phosphorylation negatively modulates AVP-induced STAT3 tyrosine phosphorylation. Serine 42-48 signal transducer and activator of transcription 3 Homo sapiens 98-103 16651438-5 2006 Of interest, the rapid down-regulation in STAT3 was consistent with an accelerated ubiquitin-dependent degradation in the Tyr(705)-phosphorylated STAT3, but not the Ser(727)-phosphorylated one, another regulator of STAT3 activity. Serine 165-168 signal transducer and activator of transcription 3 Homo sapiens 42-47 16651438-6 2006 The expression level of Ser(727)-phosphorylated STAT3 was gradually decreased by the luteolin treatment, followed by a fast and clear down-regulation in the active forms of CDK5, which can phosphorylate STAT3 at Ser(727). Serine 24-27 signal transducer and activator of transcription 3 Homo sapiens 48-53 16651438-6 2006 The expression level of Ser(727)-phosphorylated STAT3 was gradually decreased by the luteolin treatment, followed by a fast and clear down-regulation in the active forms of CDK5, which can phosphorylate STAT3 at Ser(727). Serine 24-27 signal transducer and activator of transcription 3 Homo sapiens 203-208 16651438-6 2006 The expression level of Ser(727)-phosphorylated STAT3 was gradually decreased by the luteolin treatment, followed by a fast and clear down-regulation in the active forms of CDK5, which can phosphorylate STAT3 at Ser(727). Serine 212-215 signal transducer and activator of transcription 3 Homo sapiens 48-53 16651438-6 2006 The expression level of Ser(727)-phosphorylated STAT3 was gradually decreased by the luteolin treatment, followed by a fast and clear down-regulation in the active forms of CDK5, which can phosphorylate STAT3 at Ser(727). Serine 212-215 signal transducer and activator of transcription 3 Homo sapiens 203-208 16651438-9 2006 These data suggested that luteolin targeted STAT3 through dual pathways-the ubiquitin-dependent degradation in Tyr(705)-phosphorylated STAT3 and the gradual down-regulation in Ser(727)-phosphorylated STAT3 through inactivation of CDK5, thereby triggering apoptosis via up-regulation in Fas/CD95. Serine 176-179 signal transducer and activator of transcription 3 Homo sapiens 44-49 16540667-6 2006 In non-small-cell lung cancer cells, STAT3 activity is regulated by EGFR through modulation of STAT3 serine phosphorylation. Serine 101-107 signal transducer and activator of transcription 3 Homo sapiens 95-100 15833272-8 2005 Serine phosphorylation of STAT-3 was increased by 2-fold after 15 min of cyclic strain, while tyrosine phosphorylation was increased by 2.3-fold after 60 min. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-32 16247467-4 2006 Here we show that Galpha(12) stimulates the phosphorylation of STAT3 at both Tyrosine-705 and Serine-727 residues. Serine 94-100 signal transducer and activator of transcription 3 Homo sapiens 63-68 16247467-5 2006 Studies to delineate the mechanism by which Galpha(12) stimulates STAT3 have indicated that the Tyrosine-705-phosphorylation of STAT3 involves the tyrosine kinases, Janus Kinase-3 as well as Src kinase, whereas the Serine-727 phosphorylation of STAT3 occurs via the receptor tyrosine kinase, PDGFRalpha and phosphatidylinositol 3-OH kinase pathway. Serine 215-221 signal transducer and activator of transcription 3 Homo sapiens 66-71 16247467-5 2006 Studies to delineate the mechanism by which Galpha(12) stimulates STAT3 have indicated that the Tyrosine-705-phosphorylation of STAT3 involves the tyrosine kinases, Janus Kinase-3 as well as Src kinase, whereas the Serine-727 phosphorylation of STAT3 occurs via the receptor tyrosine kinase, PDGFRalpha and phosphatidylinositol 3-OH kinase pathway. Serine 215-221 signal transducer and activator of transcription 3 Homo sapiens 128-133 16247467-5 2006 Studies to delineate the mechanism by which Galpha(12) stimulates STAT3 have indicated that the Tyrosine-705-phosphorylation of STAT3 involves the tyrosine kinases, Janus Kinase-3 as well as Src kinase, whereas the Serine-727 phosphorylation of STAT3 occurs via the receptor tyrosine kinase, PDGFRalpha and phosphatidylinositol 3-OH kinase pathway. Serine 215-221 signal transducer and activator of transcription 3 Homo sapiens 128-133 16007122-7 2005 Collectively, these results suggest that both Stat3 and AKT provide survival signals in U87 and D54 cells, and Ser/Thr phosphorylation of Stat3-TAD by the PI3K-AKT pathway negatively controls the DNA-binding function of Stat3. Serine 111-114 signal transducer and activator of transcription 3 Homo sapiens 138-143 16007122-7 2005 Collectively, these results suggest that both Stat3 and AKT provide survival signals in U87 and D54 cells, and Ser/Thr phosphorylation of Stat3-TAD by the PI3K-AKT pathway negatively controls the DNA-binding function of Stat3. Serine 111-114 signal transducer and activator of transcription 3 Homo sapiens 138-143 16005944-2 2005 However, the role of phospho-STAT3 (p-STAT3) at serine residue 727 is in large part undetermined. Serine 48-54 signal transducer and activator of transcription 3 Homo sapiens 29-34 16005944-2 2005 However, the role of phospho-STAT3 (p-STAT3) at serine residue 727 is in large part undetermined. Serine 48-54 signal transducer and activator of transcription 3 Homo sapiens 38-43 16418226-9 2006 Finally, overexpression of a STAT3 serine mutant abrogated insulin-induced STAT3 serine phosphorylation and STAT3-induced keratinocyte proliferation, whereas STAT3 tyrosine phosphorylation was induced and nuclear localization remained intact. Serine 35-41 signal transducer and activator of transcription 3 Homo sapiens 29-34 16418226-9 2006 Finally, overexpression of a STAT3 serine mutant abrogated insulin-induced STAT3 serine phosphorylation and STAT3-induced keratinocyte proliferation, whereas STAT3 tyrosine phosphorylation was induced and nuclear localization remained intact. Serine 35-41 signal transducer and activator of transcription 3 Homo sapiens 75-80 16418226-9 2006 Finally, overexpression of a STAT3 serine mutant abrogated insulin-induced STAT3 serine phosphorylation and STAT3-induced keratinocyte proliferation, whereas STAT3 tyrosine phosphorylation was induced and nuclear localization remained intact. Serine 35-41 signal transducer and activator of transcription 3 Homo sapiens 75-80 16418226-9 2006 Finally, overexpression of a STAT3 serine mutant abrogated insulin-induced STAT3 serine phosphorylation and STAT3-induced keratinocyte proliferation, whereas STAT3 tyrosine phosphorylation was induced and nuclear localization remained intact. Serine 35-41 signal transducer and activator of transcription 3 Homo sapiens 75-80 16418226-9 2006 Finally, overexpression of a STAT3 serine mutant abrogated insulin-induced STAT3 serine phosphorylation and STAT3-induced keratinocyte proliferation, whereas STAT3 tyrosine phosphorylation was induced and nuclear localization remained intact. Serine 81-87 signal transducer and activator of transcription 3 Homo sapiens 29-34 16418226-9 2006 Finally, overexpression of a STAT3 serine mutant abrogated insulin-induced STAT3 serine phosphorylation and STAT3-induced keratinocyte proliferation, whereas STAT3 tyrosine phosphorylation was induced and nuclear localization remained intact. Serine 81-87 signal transducer and activator of transcription 3 Homo sapiens 75-80 16418226-9 2006 Finally, overexpression of a STAT3 serine mutant abrogated insulin-induced STAT3 serine phosphorylation and STAT3-induced keratinocyte proliferation, whereas STAT3 tyrosine phosphorylation was induced and nuclear localization remained intact. Serine 81-87 signal transducer and activator of transcription 3 Homo sapiens 75-80 16418226-9 2006 Finally, overexpression of a STAT3 serine mutant abrogated insulin-induced STAT3 serine phosphorylation and STAT3-induced keratinocyte proliferation, whereas STAT3 tyrosine phosphorylation was induced and nuclear localization remained intact. Serine 81-87 signal transducer and activator of transcription 3 Homo sapiens 75-80 16418226-10 2006 This study indicates that PKCdelta activation is a primary regulator of STAT3 serine phosphorylation and that PKCdelta is essential in directing insulin-induced signaling in keratinocyte proliferation. Serine 78-84 signal transducer and activator of transcription 3 Homo sapiens 72-77 16219639-5 2005 ZIPK phosphorylated STAT3 on serine 727 (Ser727) and enhanced STAT3 transcriptional activity. Serine 29-35 signal transducer and activator of transcription 3 Homo sapiens 20-25 15940250-6 2005 Furthermore, the FGF-induced activation of ERK 1/2 contributed to the serine phosphorylation of STAT 3, suggesting that the signaling crosstalk between the cytokine receptor, IL-6 receptor alpha/gp 130 and the growth factor receptor tyrosine kinase, FGFR 3. Serine 70-76 signal transducer and activator of transcription 3 Homo sapiens 96-102 15833272-9 2005 Inhibition of ERK1/2 by PD98059 prevented serine phosphorylation of STAT-3, whereas inhibition of Src by PP1 prevented STAT-3 tyrosine phosphorylation. Serine 42-48 signal transducer and activator of transcription 3 Homo sapiens 68-74 15833272-10 2005 Pretreating the cells with SB202190, a specific inhibitor of p38, resulted in an increase in basal phosphorylation of ERK1/2 and a subsequent increase in basal serine phosphorylation of STAT-3. Serine 160-166 signal transducer and activator of transcription 3 Homo sapiens 186-192 15833272-12 2005 Serine phosphorylation of STAT-3 is mediated by ERK1/2, while tyrosine phosphorylation is mediated by Src. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-32 15764709-10 2005 Together, these results indicate that STAT3 enhances the efficiency of its own Ser-727 phosphorylation by acting as a scaffold for the TAK1-NLK kinases, specifically in the YXXQ motif-derived pathway. Serine 79-82 signal transducer and activator of transcription 3 Homo sapiens 38-43 15805288-2 2005 Treatment of cells with R115777 inhibited the tyrosine phosphorylation of STAT3((Tyr705)), while increasing the serine phosphorylation of STAT3((Ser727)). Serine 112-118 signal transducer and activator of transcription 3 Homo sapiens 138-143 15764709-0 2005 STAT3 regulates Nemo-like kinase by mediating its interaction with IL-6-stimulated TGFbeta-activated kinase 1 for STAT3 Ser-727 phosphorylation. Serine 120-123 signal transducer and activator of transcription 3 Homo sapiens 0-5 15764709-0 2005 STAT3 regulates Nemo-like kinase by mediating its interaction with IL-6-stimulated TGFbeta-activated kinase 1 for STAT3 Ser-727 phosphorylation. Serine 120-123 signal transducer and activator of transcription 3 Homo sapiens 114-119 15703780-5 2005 We find that CLL B cells abundantly express cytoplasmic serine phosphorylated (p)-STAT-1 and p-STAT-3, VEGF-R1/2 are physically associated with p-STAT-1 and p-STAT-3, and p-STAT-3 (but not p-STAT-1) is found in the CLL nucleus. Serine 56-62 signal transducer and activator of transcription 3 Homo sapiens 159-165 15703780-5 2005 We find that CLL B cells abundantly express cytoplasmic serine phosphorylated (p)-STAT-1 and p-STAT-3, VEGF-R1/2 are physically associated with p-STAT-1 and p-STAT-3, and p-STAT-3 (but not p-STAT-1) is found in the CLL nucleus. Serine 56-62 signal transducer and activator of transcription 3 Homo sapiens 159-165 15764709-2 2005 STAT3 requires phosphorylation on Ser-727, in addition to tyrosine phosphorylation on Tyr-705, to be transcriptionally active. Serine 34-37 signal transducer and activator of transcription 3 Homo sapiens 0-5 15653507-1 2005 Upon cytokine treatment, members of the signal transducers and activators of transcription (STAT) family of proteins are phosphorylated on tyrosine and serine sites within the carboxyl-terminal region in cells. Serine 152-158 signal transducer and activator of transcription 3 Homo sapiens 92-96 15764709-4 2005 Here, we show that TGF-beta-activated kinase 1 (TAK1) interacts with STAT3, that the TAK1-Nemo-like kinase (NLK) pathway is efficiently activated by IL-6 through the YXXQ motif, and that this is the YXXQ-mediated H7-sensitive pathway that leads to STAT3 Ser-727 phosphorylation. Serine 254-257 signal transducer and activator of transcription 3 Homo sapiens 69-74 15522880-5 2005 IL-12-induced phosphorylation of Stat3 on Ser-727 was affected by rapamycin, which may be due to the effect of rapamycin on the IL-12-induced interaction between mammalian target of rapamycin (mTOR) and Stat3. Serine 42-45 signal transducer and activator of transcription 3 Homo sapiens 33-38 15522880-5 2005 IL-12-induced phosphorylation of Stat3 on Ser-727 was affected by rapamycin, which may be due to the effect of rapamycin on the IL-12-induced interaction between mammalian target of rapamycin (mTOR) and Stat3. Serine 42-45 signal transducer and activator of transcription 3 Homo sapiens 203-208 15465816-6 2004 IRAK1 can directly use Stat3 as a substrate and cause Stat3 serine 727 phosphorylation. Serine 60-66 signal transducer and activator of transcription 3 Homo sapiens 23-28 15465816-6 2004 IRAK1 can directly use Stat3 as a substrate and cause Stat3 serine 727 phosphorylation. Serine 60-66 signal transducer and activator of transcription 3 Homo sapiens 54-59 15325847-0 2004 Activation of formyl peptide receptor-like 1 by WKYMVm induces serine phosphorylation of STAT3, which inhibits its tyrosine phosphorylation and nuclear translocation induced by hydrogen peroxide. Serine 63-69 signal transducer and activator of transcription 3 Homo sapiens 89-94 15325847-6 2004 n-butanol, a well-known phosphatidic acid (PA) acceptor, completely inhibited WKYMVm-induced STAT3 serine phosphorylation. Serine 99-105 signal transducer and activator of transcription 3 Homo sapiens 93-98 15325847-11 2004 Taken together, we found that WKYMVm stimulated FPRL1, and that this resulted in STAT3 serine phosphorylation via PLD-mediated ERK activation, and that the serine phosphorylation of STAT3 blocked hydrogen peroxide-induced STAT3 activity. Serine 156-162 signal transducer and activator of transcription 3 Homo sapiens 182-187 15325847-11 2004 Taken together, we found that WKYMVm stimulated FPRL1, and that this resulted in STAT3 serine phosphorylation via PLD-mediated ERK activation, and that the serine phosphorylation of STAT3 blocked hydrogen peroxide-induced STAT3 activity. Serine 156-162 signal transducer and activator of transcription 3 Homo sapiens 182-187 15325847-8 2004 We also found that WKYMVm stimulated extracellular signal regulated kinase (ERK), and that ERK activity is required for STAT3 serine phosphorylation. Serine 126-132 signal transducer and activator of transcription 3 Homo sapiens 120-125 15325847-10 2004 In terms of the functional aspects of the WKYMVm-induced serine phosphorylation of STAT3, we found that hydrogen peroxide-stimulated STAT3 activation was blocked by pretreating WKYMVm. Serine 57-63 signal transducer and activator of transcription 3 Homo sapiens 83-88 15325847-10 2004 In terms of the functional aspects of the WKYMVm-induced serine phosphorylation of STAT3, we found that hydrogen peroxide-stimulated STAT3 activation was blocked by pretreating WKYMVm. Serine 57-63 signal transducer and activator of transcription 3 Homo sapiens 133-138 15325847-11 2004 Taken together, we found that WKYMVm stimulated FPRL1, and that this resulted in STAT3 serine phosphorylation via PLD-mediated ERK activation, and that the serine phosphorylation of STAT3 blocked hydrogen peroxide-induced STAT3 activity. Serine 87-93 signal transducer and activator of transcription 3 Homo sapiens 81-86 14757756-2 2004 We recently revealed that p38 MAPK-mediated serine phosphorylation of both Stat1 and Stat3 is required for the induction of 15-lipoxygenase (15-LO) expression by IL-13. Serine 44-50 signal transducer and activator of transcription 3 Homo sapiens 85-90 12637318-0 2003 Serine phosphorylation of STAT3 is essential for Mcl-1 expression and macrophage survival. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-31 15115615-10 2004 Based upon these results, it is concluded that ERK1/2 negatively modulates STAT3 phosphorylation and this function is dependent on residual serine-727 (S727) of STAT3. Serine 140-146 signal transducer and activator of transcription 3 Homo sapiens 75-80 15115615-10 2004 Based upon these results, it is concluded that ERK1/2 negatively modulates STAT3 phosphorylation and this function is dependent on residual serine-727 (S727) of STAT3. Serine 140-146 signal transducer and activator of transcription 3 Homo sapiens 161-166 14715258-6 2004 Further analysis demonstrated that phosphorylation of HSP27 at serine residue 78 was induced by Stat3-C in TERT breast cells as well as in MCF-10A and MDA-MB-453 breast cells. Serine 63-69 signal transducer and activator of transcription 3 Homo sapiens 96-101 14715258-10 2004 Taken together, our findings demonstrate that constitutively activated Stat3 up-regulates HSP27 and may facilitate phosphorylation of HSP27 at serine residue 78. Serine 143-149 signal transducer and activator of transcription 3 Homo sapiens 71-76 12913253-8 2003 Tyr phosphorylation of Erk and Ser phosphorylation of STAT-3 were inhibited by PD98059, while Tyr phosphorylation of STAT-3 was not inhibited by PD98059. Serine 31-34 signal transducer and activator of transcription 3 Homo sapiens 54-60 12913253-10 2003 These results first indicate that Erk1/2 and STAT-3 regulate CCR5 expression, and that Erk-mediated phosphorylation of Ser is required for full stimulation of STAT-3 in CCR5 expression. Serine 119-122 signal transducer and activator of transcription 3 Homo sapiens 159-165 12681450-0 2003 IL-2 activation of a PI3K-dependent STAT3 serine phosphorylation pathway in primary human T cells. Serine 42-48 signal transducer and activator of transcription 3 Homo sapiens 36-41 12821944-6 2003 To study the possible relevance of STAT3 activation by BCR-ABL in human CML, Western blot analyses performed on the CD34+ cells, purified from CML patients at different stages of their disease, also demonstrated increased levels of STAT3 proteins phosphorylated both on tyrosine and serine residues. Serine 283-289 signal transducer and activator of transcription 3 Homo sapiens 232-237 12684046-6 2003 Subsequently, STAT-3 translocated to the nucleus where serine 727 (S727) was phosphorylated, establishing a transcriptionally active STAT-3 transcription factor. Serine 55-61 signal transducer and activator of transcription 3 Homo sapiens 14-20 12710894-4 2003 The ability of IL-6 to promote transcription of CRP is mediated, in large part, by activation of the transcription factor STAT3; this activation requires both a tyrosine phosphorylation (mediated by the IL-6 receptor complex) and a serine phosphorylation (Ser-727), the origin of which has been more obscure. Serine 232-238 signal transducer and activator of transcription 3 Homo sapiens 122-127 12710894-4 2003 The ability of IL-6 to promote transcription of CRP is mediated, in large part, by activation of the transcription factor STAT3; this activation requires both a tyrosine phosphorylation (mediated by the IL-6 receptor complex) and a serine phosphorylation (Ser-727), the origin of which has been more obscure. Serine 256-259 signal transducer and activator of transcription 3 Homo sapiens 122-127 12710894-5 2003 There is new evidence that, when hepatocytes are exposed to IL-6, the consequent serine phosphorylation of STATS is mediated by a signal transduction pathway in which the G-protein Rac-1 plays an obligate role. Serine 81-87 signal transducer and activator of transcription 3 Homo sapiens 107-112 12681450-5 2003 However, the coupling of STAT3 serine phosphorylation to PI3K in response to IL-2 has yet to be shown in either T cell lines or primary human T cells. Serine 31-37 signal transducer and activator of transcription 3 Homo sapiens 25-30 12681450-7 2003 Moreover, these inhibitors significantly reduce IL-2-triggered STAT3 serine phosphorylation without affecting STAT5 serine phosphorylation. Serine 69-75 signal transducer and activator of transcription 3 Homo sapiens 63-68 12748293-5 2003 We found that IL-13 induced a time-dependent serine phosphorylation of both Stat1 and Stat3, detectable at 15 min after IL-13 treatment. Serine 45-51 signal transducer and activator of transcription 3 Homo sapiens 86-91 12748293-7 2003 SB202190, a p38 MAPK-specific inhibitor, markedly inhibited IL-13-induced Stat1 and Stat3 serine phosphorylation as well as DNA binding. Serine 90-96 signal transducer and activator of transcription 3 Homo sapiens 84-89 12748293-9 2003 Taken together, our results provide the first evidence that IL-13 induces p38 MAPK phosphorylation/activation, which regulates Stat1 and Stat3 serine 727 phosphorylation. Serine 143-149 signal transducer and activator of transcription 3 Homo sapiens 137-142 12763138-0 2003 Erythropoietin-induced serine 727 phosphorylation of STAT3 in erythroid cells is mediated by a MEK-, ERK-, and MSK1-dependent pathway. Serine 23-29 signal transducer and activator of transcription 3 Homo sapiens 53-58 12763138-4 2003 In the present study, we investigated which molecular pathways mediate the STAT3 serine 727 phosphorylation and the functional implications of this phosphorylation. Serine 81-87 signal transducer and activator of transcription 3 Homo sapiens 75-80 12763138-5 2003 METHODS: The EPO-dependent erythroid cell line ASE2 was used to investigate which signaling routes were involved in the STAT3 serine 727 phosphorylation. Serine 126-132 signal transducer and activator of transcription 3 Homo sapiens 120-125 12763138-8 2003 RESULTS: Western blotting of extracts of cells exposed to various chemical inhibitors revealed that the MEK inhibitors PD98059 and U0126 abrogated the EPO-mediated STAT3 serine 727 phosphorylation without an effect on tyrosine phosphorylation. Serine 170-176 signal transducer and activator of transcription 3 Homo sapiens 164-169 12763138-9 2003 Further analysis showed that MSK1 is activated downstream of ERK, and retroviral transductions with kinase-inactive MSK1 revealed that MSK1 is necessary for STAT3 serine phosphorylation. Serine 163-169 signal transducer and activator of transcription 3 Homo sapiens 157-162 12763138-10 2003 Furthermore, the STAT3-mediated transactivation was reduced by blocking the STAT3 serine phosphorylation with the MEK inhibitor U0126 or by expression of kinase-inactive MSK1. Serine 82-88 signal transducer and activator of transcription 3 Homo sapiens 17-22 12763138-10 2003 Furthermore, the STAT3-mediated transactivation was reduced by blocking the STAT3 serine phosphorylation with the MEK inhibitor U0126 or by expression of kinase-inactive MSK1. Serine 82-88 signal transducer and activator of transcription 3 Homo sapiens 76-81 12763138-11 2003 CONCLUSIONS: The EPO-induced STAT3 serine 727 phosphorylation is mediated by a pathway involving MEK, ERK, and MSK1. Serine 35-41 signal transducer and activator of transcription 3 Homo sapiens 29-34 12763138-12 2003 Furthermore, serine phosphorylation of STAT3 augments the transactivational potential of STAT3. Serine 13-19 signal transducer and activator of transcription 3 Homo sapiens 39-44 12763138-12 2003 Furthermore, serine phosphorylation of STAT3 augments the transactivational potential of STAT3. Serine 13-19 signal transducer and activator of transcription 3 Homo sapiens 89-94 12562765-1 2003 Phosphorylation at Ser(727) is known to be required for complete activation of STAT3 by diverse stimuli including UV irradiation, but the kinase(s) responsible for phosphorylating STAT3 (Ser(727)) is still not well discerned. Serine 19-22 signal transducer and activator of transcription 3 Homo sapiens 79-84 12562765-1 2003 Phosphorylation at Ser(727) is known to be required for complete activation of STAT3 by diverse stimuli including UV irradiation, but the kinase(s) responsible for phosphorylating STAT3 (Ser(727)) is still not well discerned. Serine 187-190 signal transducer and activator of transcription 3 Homo sapiens 79-84 12361954-1 2002 The transcriptional regulation of Stat proteins is controlled through their C-terminal domains, which harbor both a tyrosine phosphorylation site, required for dimerization and subsequent nuclear translocation, and a serine phosphorylation site, required for maximum transcriptional activity. Serine 217-223 signal transducer and activator of transcription 3 Homo sapiens 34-38 12562765-1 2003 Phosphorylation at Ser(727) is known to be required for complete activation of STAT3 by diverse stimuli including UV irradiation, but the kinase(s) responsible for phosphorylating STAT3 (Ser(727)) is still not well discerned. Serine 187-190 signal transducer and activator of transcription 3 Homo sapiens 180-185 12562765-2 2003 In the present study, we observed that activation of ATM is required for a UVA-stimulated increase in Ser(727) phosphorylation of STAT3 as well as in activation and phosphorylation of p90 ribosomal protein S6 kinases (RSKs). Serine 102-105 signal transducer and activator of transcription 3 Homo sapiens 130-135 12562765-4 2003 Furthermore, we provide evidence that RSK2-deficient cells were defective for UV-induced Ser(727) phosphorylation of STAT3, and the defect was restored after ectopic expression of transfected full-length RSK2. Serine 89-92 signal transducer and activator of transcription 3 Homo sapiens 117-122 12562765-8 2003 Taken together, our results demonstrate that the STAT3 phosphorylation at Ser(727) is triggered by active RSK2 or JNK1 in the presence of a downstream kinase or a cofactor, and thereby the intracellular phosphorylation process is stimulated through a signaling pathway involving ATM, MAPKs, RSK2, and an as yet unidentified kinase or cofactor. Serine 74-77 signal transducer and activator of transcription 3 Homo sapiens 49-54 12562765-9 2003 Additionally, RSK2-mediated phosphorylation of STAT3 (Ser(727)) was further determined to be required for basal and UVA-stimulated STAT3 transcriptional activities. Serine 54-57 signal transducer and activator of transcription 3 Homo sapiens 47-52 12562765-9 2003 Additionally, RSK2-mediated phosphorylation of STAT3 (Ser(727)) was further determined to be required for basal and UVA-stimulated STAT3 transcriptional activities. Serine 54-57 signal transducer and activator of transcription 3 Homo sapiens 131-136 12474230-5 2003 The expression of the two phosphorylated (i.e. active) forms of STAT3, pSTAT3-tyr (phosphorylated at the tyrosine(705) residue) and pSTAT3-ser (phosphorylated at the serine(727) residue), was assessed in four MCL cell lines and 12 MCL tumours using western blots and/or immunofluorescence staining techniques. Serine 166-172 signal transducer and activator of transcription 3 Homo sapiens 64-69 11335711-7 2001 Studying the kinetics of STAT3 and PKCdelta phosphorylation in cytoplasmic and nuclear fractions revealed that STAT3 Tyr-705 phosphorylation and nuclear translocation precedes PKCdelta Thr-505 and STAT3 Ser-727 phosphorylation. Serine 203-206 signal transducer and activator of transcription 3 Homo sapiens 111-116 12351686-3 2002 In endothelial cells, Stat5 and Stat3 are rapidly phosphorylated on both tyrosine and serine residues in response to 17beta-estradiol, and nuclear translocation is subsequently induced. Serine 86-92 signal transducer and activator of transcription 3 Homo sapiens 32-37 12130710-8 2002 Also using immunohistochemistry to identify potential intracellular mechanisms associated with alcohol-induced c-Fos expression in Edinger-Westphal, we show time-dependent increases in serine 727 phospho-signal transducer and activator of transcription 3 (Stat3) but no changes in phospho-cAMP response element-binding protein and phospho-Elk1. Serine 185-191 signal transducer and activator of transcription 3 Homo sapiens 256-261 12130710-9 2002 Time-dependent increases in phospho-extracellular signal-regulated kinase (ERK) 1/2 were found to occur simultaneously with increases in serine 727 phospho-Stat3. Serine 137-143 signal transducer and activator of transcription 3 Homo sapiens 156-161 11922392-8 2002 Serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) was observed after FGF-1 treatment and pretreatment with 20 microM PD98059-abolished STAT3 phosphorylation. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-76 11922392-8 2002 Serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) was observed after FGF-1 treatment and pretreatment with 20 microM PD98059-abolished STAT3 phosphorylation. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 78-83 11922392-8 2002 Serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) was observed after FGF-1 treatment and pretreatment with 20 microM PD98059-abolished STAT3 phosphorylation. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 170-175 11922392-10 2002 We propose that the FGF-1-induced signaling pathway that leads to promatrilysin expression is ERK-dependent and leads to phosphorylation of Ser-727 on STAT3, phosphorylated STAT3, then binds and transactivates the matrilysin promoter. Serine 140-143 signal transducer and activator of transcription 3 Homo sapiens 151-156 11598209-0 2001 Simultaneous tyrosine and serine phosphorylation of STAT3 transcription factor is involved in Rho A GTPase oncogenic transformation. Serine 26-32 signal transducer and activator of transcription 3 Homo sapiens 52-57 11598209-2 2001 Here we report a novel signaling pathway whereby RhoA can efficiently modulate Stat3 transcriptional activity by inducing its simultaneous tyrosine and serine phosphorylation. Serine 152-158 signal transducer and activator of transcription 3 Homo sapiens 79-84 11598209-4 2001 Furthermore, cooperation of both tyrosine as well as serine phosphorylation is necessary for full activation of Stat3. Serine 53-59 signal transducer and activator of transcription 3 Homo sapiens 112-117 11598209-9 2001 Taken together, these results indicate that Stat3 is an important player in RhoA-mediated oncogenic transformation, which requires simultaneous phosphorylation at both tyrosine and serine residues by specific signaling events triggered by RhoA effectors. Serine 181-187 signal transducer and activator of transcription 3 Homo sapiens 44-49 12152985-7 2002 Among the biochemical and molecular events mediated by these cytokines are the down-regulation of p27kip1 and the independent phosphorylation of STAT3 on tyrosine and serine residues. Serine 167-173 signal transducer and activator of transcription 3 Homo sapiens 145-150 11875080-8 2002 STAT1 serine phosphorylation was perturbed by inhibition of ERK and p38 pathways, whereas only inhibition of ERK activation blocked STAT3 serine phosphorylation in response to EPO. Serine 138-144 signal transducer and activator of transcription 3 Homo sapiens 132-137 11553624-1 2001 Phosphorylation of Tyr(705) and Ser(727) of signal transducer and activator of transcription 3 (STAT3) are known to be required for maximal activation by diverse stimuli. Serine 32-35 signal transducer and activator of transcription 3 Homo sapiens 44-94 11553624-1 2001 Phosphorylation of Tyr(705) and Ser(727) of signal transducer and activator of transcription 3 (STAT3) are known to be required for maximal activation by diverse stimuli. Serine 32-35 signal transducer and activator of transcription 3 Homo sapiens 96-101 11553624-3 2001 But the mechanism for STAT3 (Ser(727)) phosphorylation is not well understood. Serine 29-32 signal transducer and activator of transcription 3 Homo sapiens 22-27 11553624-4 2001 Here, we provide evidence that UVA-induced phosphorylation of STAT3 at Ser(727) is inhibited by pretreatment of JB6 cells with PD98059 or SB202190. Serine 71-74 signal transducer and activator of transcription 3 Homo sapiens 62-67 11553624-5 2001 Phosphorylation of STAT3 (Ser(727)) is also markedly prevented by a dominant negative mutant of ERK2, c-Jun N-terminal kinase 1 (JNK1), or p38 kinase and in knockout Jnk1(-/-) or Jnk2(-/-) cells. Serine 26-29 signal transducer and activator of transcription 3 Homo sapiens 19-24 11553624-6 2001 Furthermore, STAT3 (Ser(727)) phosphorylation is suppressed by C- or N-terminal "kinase-dead" mutants of mitogen- and stress-activated protein kinase 1 (MSK1), a downstream kinase of ERKs and p38 kinase, and H89, a potential MSK1 inhibitor. Serine 20-23 signal transducer and activator of transcription 3 Homo sapiens 13-18 11553624-7 2001 In vitro experiments showed that active MSK1 and JNKs, but not ERKs or p38 kinase, phosphorylate STAT3 (Ser(727)). Serine 104-107 signal transducer and activator of transcription 3 Homo sapiens 97-102 11553624-9 2001 Overall, these results suggest that UVA-induced Ser(727) phosphorylation of STAT3 may occur through MSK1 and JNKs. Serine 48-51 signal transducer and activator of transcription 3 Homo sapiens 76-81 11335711-0 2001 Sequential activation of Rac-1, SEK-1/MKK-4, and protein kinase Cdelta is required for interleukin-6-induced STAT3 Ser-727 phosphorylation and transactivation. Serine 115-118 signal transducer and activator of transcription 3 Homo sapiens 109-114 11335711-1 2001 Activation of signal transducer and activator of transcription 3 (STAT3) by interleukin-6 (IL-6) involves phosphorylation of Tyr-705 and Ser-727, both of which are critical for STAT3 transactivation. Serine 137-140 signal transducer and activator of transcription 3 Homo sapiens 14-64 11335711-1 2001 Activation of signal transducer and activator of transcription 3 (STAT3) by interleukin-6 (IL-6) involves phosphorylation of Tyr-705 and Ser-727, both of which are critical for STAT3 transactivation. Serine 137-140 signal transducer and activator of transcription 3 Homo sapiens 66-71 11335711-1 2001 Activation of signal transducer and activator of transcription 3 (STAT3) by interleukin-6 (IL-6) involves phosphorylation of Tyr-705 and Ser-727, both of which are critical for STAT3 transactivation. Serine 137-140 signal transducer and activator of transcription 3 Homo sapiens 177-182 11335711-5 2001 Inhibition of PKCdelta using rottlerin (6 microm) or by overexpression of dominant negative PKCdelta demonstrates that PKCdelta kinase activity is required for STAT3 Ser-727 phosphorylation and transactivation but not for STAT3 Tyr-705 phosphorylation or nuclear import. Serine 166-169 signal transducer and activator of transcription 3 Homo sapiens 160-165 11335711-7 2001 Studying the kinetics of STAT3 and PKCdelta phosphorylation in cytoplasmic and nuclear fractions revealed that STAT3 Tyr-705 phosphorylation and nuclear translocation precedes PKCdelta Thr-505 and STAT3 Ser-727 phosphorylation. Serine 203-206 signal transducer and activator of transcription 3 Homo sapiens 111-116 11335711-8 2001 Furthermore, the IL-6-induced PKCdelta Thr-505 and STAT3 Ser-727 phosphorylation were only observed in nuclear fractions of HepG2 cells. Serine 57-60 signal transducer and activator of transcription 3 Homo sapiens 51-56 11335711-10 2001 Our results further indicate that PKCdelta-mediated STAT3 Ser-727 phosphorylation is mainly a nuclear event. Serine 58-61 signal transducer and activator of transcription 3 Homo sapiens 52-57 11278353-3 2001 Ser-727 phosphorylation of Stat3 plays an additional role in the regulation of transcription. Serine 0-3 signal transducer and activator of transcription 3 Homo sapiens 27-32 11278353-6 2001 Kinase-inactive MEKK1 inhibits Stat3 phosphorylation on tyrosine and serine, and its transcriptional activity stimulated by epidermal growth factor and platelet-derived growth factor in different cell types. Serine 69-75 signal transducer and activator of transcription 3 Homo sapiens 31-36 11278353-7 2001 In contrast, active MEKK1 induces Stat3 tyrosine and serine phosphorylation leading to a functionally active Stat3 capable of binding DNA and enhancing transcription. Serine 53-59 signal transducer and activator of transcription 3 Homo sapiens 109-114 11322950-2 2001 Here, we describe that the 65 C-terminal amino acids of STAT3 can function as an independent transcription activation domain (TAD), particularly when a negative charge is introduced at position 727 by mutation of the serine residue into aspartate. Serine 217-223 signal transducer and activator of transcription 3 Homo sapiens 56-61 11429693-8 2001 Thus, the YXXQ motif regulates STAT3 activities in two ways in response to even a low concentration of IL-6: it recruits STAT3 to the receptor for tyrosine phosphorylation, and activates an unidentified H7-sensitive pathway leading to the serine phosphorylation of STAT3. Serine 239-245 signal transducer and activator of transcription 3 Homo sapiens 31-36 11429693-8 2001 Thus, the YXXQ motif regulates STAT3 activities in two ways in response to even a low concentration of IL-6: it recruits STAT3 to the receptor for tyrosine phosphorylation, and activates an unidentified H7-sensitive pathway leading to the serine phosphorylation of STAT3. Serine 239-245 signal transducer and activator of transcription 3 Homo sapiens 121-126 11429693-8 2001 Thus, the YXXQ motif regulates STAT3 activities in two ways in response to even a low concentration of IL-6: it recruits STAT3 to the receptor for tyrosine phosphorylation, and activates an unidentified H7-sensitive pathway leading to the serine phosphorylation of STAT3. Serine 239-245 signal transducer and activator of transcription 3 Homo sapiens 121-126 11071637-9 2000 The pathways by which SCF and G-CSF lead to serine phosphorylation of STAT3 are distinct and are partially dependent on phosphatidylinositol-3 kinase and ERKs, pathways that are also necessary for the synergistic effects of SCF and G-CSF on proliferation and c-fos induction. Serine 44-50 signal transducer and activator of transcription 3 Homo sapiens 70-75 11150545-3 2001 We have applied this method for screening kinases which phosphorylate STAT3 at serine(727). Serine 79-85 signal transducer and activator of transcription 3 Homo sapiens 70-75 11150545-4 2001 In this screening, antibody (PS727 antibody) specifically recognizing STAT3 in which serine(727) is phosphorylated was first prepared. Serine 85-91 signal transducer and activator of transcription 3 Homo sapiens 70-75 11150545-5 2001 Escherichia coli, bacteria expressing a serine(727)-containing fragment of STAT3 which was fused to glutathione-S-transferase (GST) (GST-STAT3-WT) were infected by lambda phage cDNA expression libraries. Serine 40-46 signal transducer and activator of transcription 3 Homo sapiens 75-80 11150545-8 2001 These kinases have a potential to phosphorylate serine(727) in STAT3 protein also in mammalian cells. Serine 48-54 signal transducer and activator of transcription 3 Homo sapiens 63-68 10777558-6 2000 Last, the IFNalpha-induced serine phosphorylation of STAT1 and STAT3 is not inhibited by piceatannol but is sensitive to the Src kinase-specific inhibitor PP2. Serine 27-33 signal transducer and activator of transcription 3 Homo sapiens 63-68 11021801-3 2000 Dominant negative Rac1 inhibited STAT3 activation by growth factors, whereas activated Rac1 stimulated STAT3 phosphorylation on both tyrosine and serine residues. Serine 146-152 signal transducer and activator of transcription 3 Homo sapiens 103-108 10777558-7 2000 Thus, our results not only demonstrate that the IFNalpha/beta receptor utilizes distinct mechanisms to trigger the tyrosine phosphorylation of specific STAT proteins, but they also indicate a diverging pathway that leads to the serine phosphorylation of STAT1 and STAT3. Serine 228-234 signal transducer and activator of transcription 3 Homo sapiens 264-269 10660304-2 2000 Crosstalk occurs between these pathways, because studies have shown that STAT3 requires phosphorylation on tyrosine and serine residues by independent protein kinase activities for maximal activation of target gene transcription. Serine 120-126 signal transducer and activator of transcription 3 Homo sapiens 73-78 10727406-1 2000 In the present study, signal transducer and activator of transcription 3 (STAT3) Ser(727) phosphorylation and transactivation was investigated in relation to activation of mitogen-activated protein (MAP) kinase family members including extracellular-signal-regulated protein kinase (ERK)-1, c-Jun N-terminal kinase (JNK)-1 and p38 ("reactivating kinase") in response to interleukin (IL)-6 stimulation. Serine 81-84 signal transducer and activator of transcription 3 Homo sapiens 22-72 10727406-1 2000 In the present study, signal transducer and activator of transcription 3 (STAT3) Ser(727) phosphorylation and transactivation was investigated in relation to activation of mitogen-activated protein (MAP) kinase family members including extracellular-signal-regulated protein kinase (ERK)-1, c-Jun N-terminal kinase (JNK)-1 and p38 ("reactivating kinase") in response to interleukin (IL)-6 stimulation. Serine 81-84 signal transducer and activator of transcription 3 Homo sapiens 74-79 10542198-6 1999 In contrast, phosphorylation of STAT3 occurs exclusively on serine and is sensitive to inhibitors of the PI3-kinase and the ERK1/2 pathways. Serine 60-66 signal transducer and activator of transcription 3 Homo sapiens 32-37 10485875-3 1999 Recent reports suggest that serine phosphorylation of STATs also is involved in the regulation of STAT-mediated gene transcription. Serine 28-34 signal transducer and activator of transcription 3 Homo sapiens 54-59 10521505-2 1999 Recently, serine phosphorylation of Stat3 on Ser-727 by ERK has been identified in response to epidermal growth factor (EGF). Serine 10-16 signal transducer and activator of transcription 3 Homo sapiens 36-41 10521505-2 1999 Recently, serine phosphorylation of Stat3 on Ser-727 by ERK has been identified in response to epidermal growth factor (EGF). Serine 45-48 signal transducer and activator of transcription 3 Homo sapiens 36-41 10521505-3 1999 Here, we report that Ser-727 phosphorylation of Stat3 can also be induced by JNK and activated either by stress or by its upstream kinase and that various stress treatments induce serine phosphorylation of Stat3 in the absence of tyrosine phosphorylation. Serine 21-24 signal transducer and activator of transcription 3 Homo sapiens 48-53 10521505-3 1999 Here, we report that Ser-727 phosphorylation of Stat3 can also be induced by JNK and activated either by stress or by its upstream kinase and that various stress treatments induce serine phosphorylation of Stat3 in the absence of tyrosine phosphorylation. Serine 21-24 signal transducer and activator of transcription 3 Homo sapiens 206-211 10521505-3 1999 Here, we report that Ser-727 phosphorylation of Stat3 can also be induced by JNK and activated either by stress or by its upstream kinase and that various stress treatments induce serine phosphorylation of Stat3 in the absence of tyrosine phosphorylation. Serine 180-186 signal transducer and activator of transcription 3 Homo sapiens 48-53 10521505-3 1999 Here, we report that Ser-727 phosphorylation of Stat3 can also be induced by JNK and activated either by stress or by its upstream kinase and that various stress treatments induce serine phosphorylation of Stat3 in the absence of tyrosine phosphorylation. Serine 180-186 signal transducer and activator of transcription 3 Homo sapiens 206-211 10521505-8 1999 Our results suggest that Stat3 is a target of JNK that may regulate Stat3 activity via both Ser-727 phosphorylation-dependent and -independent mechanisms. Serine 92-95 signal transducer and activator of transcription 3 Homo sapiens 25-30 10521505-8 1999 Our results suggest that Stat3 is a target of JNK that may regulate Stat3 activity via both Ser-727 phosphorylation-dependent and -independent mechanisms. Serine 92-95 signal transducer and activator of transcription 3 Homo sapiens 68-73 10542280-5 1999 The extent of STAT1 and STAT3 tyrosine and serine phosphorylation are also increased under HG conditions. Serine 43-49 signal transducer and activator of transcription 3 Homo sapiens 24-29 10523640-4 1999 Although the Src tyrosine kinase induces constitutive Stat3 phosphorylation on tyrosine, activation of Stat3-mediated gene regulation requires both tyrosine and serine phosphorylation of Stat3. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 103-108 10523640-4 1999 Although the Src tyrosine kinase induces constitutive Stat3 phosphorylation on tyrosine, activation of Stat3-mediated gene regulation requires both tyrosine and serine phosphorylation of Stat3. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 103-108 10523640-8 1999 Furthermore, inhibition of p38 and JNK activities suppresses constitutive Stat3 serine phosphorylation and Stat3-mediated gene regulation. Serine 80-86 signal transducer and activator of transcription 3 Homo sapiens 74-79 10523640-9 1999 In vitro kinase assays with purified full-length Stat3 as the substrate show that both JNK and p38 can phosphorylate Stat3 on serine. Serine 126-132 signal transducer and activator of transcription 3 Homo sapiens 49-54 10523640-9 1999 In vitro kinase assays with purified full-length Stat3 as the substrate show that both JNK and p38 can phosphorylate Stat3 on serine. Serine 126-132 signal transducer and activator of transcription 3 Homo sapiens 117-122 10523640-10 1999 Moreover, inhibition of p38 activity and thus of Stat3 serine phosphorylation results in suppression of transformation by v-Src but not v-Ras, consistent with a requirement for Stat3 serine phosphorylation in Src transformation. Serine 55-61 signal transducer and activator of transcription 3 Homo sapiens 49-54 10523640-10 1999 Moreover, inhibition of p38 activity and thus of Stat3 serine phosphorylation results in suppression of transformation by v-Src but not v-Ras, consistent with a requirement for Stat3 serine phosphorylation in Src transformation. Serine 183-189 signal transducer and activator of transcription 3 Homo sapiens 49-54 10523640-10 1999 Moreover, inhibition of p38 activity and thus of Stat3 serine phosphorylation results in suppression of transformation by v-Src but not v-Ras, consistent with a requirement for Stat3 serine phosphorylation in Src transformation. Serine 183-189 signal transducer and activator of transcription 3 Homo sapiens 177-182 10485875-5 1999 We show that an inhibitor of protein phosphatases (PPs) PP1/PP2A, calyculin A, induces (i) phosphorylation of STAT3 on serine and threonine residues, (ii) inhibition of STAT3 tyrosine phosphorylation and DNA binding activity, and (iii) relocation of STAT3 from the nucleus to the cytoplasm. Serine 119-125 signal transducer and activator of transcription 3 Homo sapiens 110-115 10446219-2 1999 Serine phosphorylation of Stat3 by mitogen-activated protein kinase has also been observed in cells responding to epidermal growth factor and shown to affect its tyrosine phosphorylation and transcriptional activity. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-31 10446219-3 1999 Serine phosphorylation of Stat3 is also induced by interleukin-6 (IL-6) stimulation, which is shown to be independent of mitogen-activated protein kinase and sensitive to the Ser/Thr kinase inhibitor H7. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-31 10446219-4 1999 In this study, we investigated whether protein kinase C (PKC) is the kinase that is induced and responsible for Stat3 serine phosphorylation by IL-6 stimulation and which isoform of PKCs is likely to be involved. Serine 118-124 signal transducer and activator of transcription 3 Homo sapiens 112-117 10446219-6 1999 Furthermore, Stat3 was phosphorylated by PKC delta in vivo on Ser-727, which could be inhibited either by a specific PKC delta inhibitor or by a dominant-negative mutant of PKC delta. Serine 62-65 signal transducer and activator of transcription 3 Homo sapiens 13-18 10347203-6 1999 Serine phosphorylation of STAT3 was only apparent after somatostatin treatment and was abolished by pertussis toxin or PD 98059, together with the associated increases in proliferation. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-31 10438903-2 1999 Here, we show that CD3 ligation by mAb and Staphylococcal enterotoxin (SE) induce a rapid, gradually accumulating, long-lasting tyrosine, and serine phosphorylation of Stat3 (but not Stat5) in allogen-specific human CD4+ T cell lines. Serine 142-148 signal transducer and activator of transcription 3 Homo sapiens 168-173 10438903-3 1999 In contrast, IL-2 induces a rapid and transient tyrosine and serine phosphorylation of Stat3. Serine 61-67 signal transducer and activator of transcription 3 Homo sapiens 87-92 10438903-6 1999 In conclusion, we show that CD3 ligation by mAb and SE triggers a rapid, PP1-sensitive tyrosine and serine phosphorylation of Stat3 in human CD4+ T cells. Serine 100-106 signal transducer and activator of transcription 3 Homo sapiens 126-131 10417333-0 1999 Extracellular signal-regulated protein kinase (ERK)-dependent and ERK-independent pathways target STAT3 on serine-727 in human neutrophils stimulated by chemotactic factors and cytokines. Serine 107-113 signal transducer and activator of transcription 3 Homo sapiens 98-103 10417333-3 1999 Here we demonstrate, with a site-specific antibody, that STAT3 is phosphorylated on Ser-727 in human neutrophils stimulated with chemotactic factors (N-formyl-methionyl-leucyl-phenylalanine and complement C5a), cytokines [granulocyte/macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF)], or a protein kinase C activator (PMA). Serine 84-87 signal transducer and activator of transcription 3 Homo sapiens 57-62 10417333-4 1999 (2-Amino-3"-methoxyphenyl)oxanaphthalen-4-one (PD 98059), an inhibitor of extracellular signal-regulated protein kinase (ERK) activation, blocked the serine phosphorylation of STAT3 induced by chemotactic factors or PMA. Serine 150-156 signal transducer and activator of transcription 3 Homo sapiens 176-181 10417333-7 1999 We propose that neutrophils use both ERK-dependent and ERK-independent pathways to phosphorylate Ser-727 on STAT3. Serine 97-100 signal transducer and activator of transcription 3 Homo sapiens 108-113 10391929-6 1999 In addition, we show that Ang II-induced serine phosphorylation of STAT3 in VSMCs is mediated by mitogen-activated protein kinase and that dephosphorylation is mediated by protein phosphatase 2A (PP2A). Serine 41-47 signal transducer and activator of transcription 3 Homo sapiens 67-72 10347203-8 1999 We conclude that the difference in the magnitude of the proliferative response evoked by the two agonists at the sst4 receptor can be accounted for by their differential ability to phosphorylate STAT3 on serine residues and supports the concept that selective signaling can be achieved through pharmacological diversity. Serine 204-210 signal transducer and activator of transcription 3 Homo sapiens 195-200 9872331-2 1998 Recent studies have indicated that STATs are also phosphorylated by MAPK, or extracellular signal-regulated kinase (ERK) on serine. Serine 124-130 signal transducer and activator of transcription 3 Homo sapiens 35-40 10201984-5 1999 The functional synergy between IL-2 and IL-12 is also associated with a prominent increase in STAT1 and STAT3 serine phosphorylation over that observed with IL-12 or IL-2 alone. Serine 110-116 signal transducer and activator of transcription 3 Homo sapiens 104-109 10201984-7 1999 A specific inhibitor of p38 MAP kinase completely inhibits the serine phosphorylation of STAT1 and STAT3 induced by IL-12 and IL-2 and abrogates the functional synergy between IL-12 and IL-2 without affecting STAT tyrosine phosphorylation. Serine 63-69 signal transducer and activator of transcription 3 Homo sapiens 99-104 9872331-6 1998 ERK2 phosphorylates Stat3 on three serine-containing peptides and decreases its tyrosine phosphorylation induced by EGF treatment. Serine 35-41 signal transducer and activator of transcription 3 Homo sapiens 20-25 9558728-11 1998 In addition to the tyrosine phosphorylation we have observed that IL-6 also induces a serine phosphorylation of STAT3. Serine 86-92 signal transducer and activator of transcription 3 Homo sapiens 112-117 9736697-8 1998 MEKs and ERKs inhibited IL-6 activation of Stat3 harboring a mutation at serine-727, the major site for serine phosphorylation, similar to inhibition of wild-type Stat3, and inhibited Janus kinases Jak1 and Jak2 upstream of Stat3 in the Jak-STAT-signaling pathway. Serine 73-79 signal transducer and activator of transcription 3 Homo sapiens 43-48 9736697-8 1998 MEKs and ERKs inhibited IL-6 activation of Stat3 harboring a mutation at serine-727, the major site for serine phosphorylation, similar to inhibition of wild-type Stat3, and inhibited Janus kinases Jak1 and Jak2 upstream of Stat3 in the Jak-STAT-signaling pathway. Serine 104-110 signal transducer and activator of transcription 3 Homo sapiens 43-48 9668040-2 1998 In addition to being tyrosine-phosphorylated, several signal transducers and activators of transcription (Stats), including Stat1alpha, Stat3, and Stat4, are phosphorylated on a conserved serine residue, which is a consensus phosphorylation site for mitogen-activated protein kinases (MAPKs). Serine 188-194 signal transducer and activator of transcription 3 Homo sapiens 136-141 9558728-13 1998 We propose that the STAT3 serine phosphorylation is required for transactivation of IL-6 target genes which is also inhibited by H7. Serine 26-32 signal transducer and activator of transcription 3 Homo sapiens 20-25 9399961-0 1997 B lymphocytes from patients with chronic lymphocytic leukemia contain signal transducer and activator of transcription (STAT) 1 and STAT3 constitutively phosphorylated on serine residues. Serine 171-177 signal transducer and activator of transcription 3 Homo sapiens 132-137 9399961-3 1997 To assess the phosphorylation of serine residues of STAT1 and STAT3 in CLL cells, we raised antibodies that specifically recognize the form of STAT1 phosphorylated on ser-727 and the form of STAT3 phosphorylated on ser-727. Serine 33-39 signal transducer and activator of transcription 3 Homo sapiens 191-196 9399961-4 1997 We found that in 100% of patients with CLL (n = 32), STAT1 and STAT3 were constitutively phosphorylated on serine. Serine 107-113 signal transducer and activator of transcription 3 Homo sapiens 63-68 9399961-6 1997 Serine phosphorylation of STAT1 and STAT3 was seen occasionally in other leukemias, but it was a universal finding only in CLL. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 36-41 9399961-9 1997 In summary, the constitutive serine phosphorylation of STAT1 and STAT3 is present in all CLL samples tested to date, although the physiologic significance of this modification remains to be determined. Serine 29-35 signal transducer and activator of transcription 3 Homo sapiens 65-70 9322921-0 1997 Signal transducer and activator of transcription-3 serine phosphorylation by insulin is mediated by a Ras/Raf/MEK-dependent pathway. Serine 51-57 signal transducer and activator of transcription 3 Homo sapiens 0-50 9343414-0 1997 STAT3 serine phosphorylation by ERK-dependent and -independent pathways negatively modulates its tyrosine phosphorylation. Serine 6-12 signal transducer and activator of transcription 3 Homo sapiens 0-5 9343414-1 1997 Recent studies have indicated that serine phosphorylation regulates the activities of STAT1 and STAT3. Serine 35-41 signal transducer and activator of transcription 3 Homo sapiens 96-101 9343414-4 1997 We provide in vitro and in vivo evidence that the ERK family of mitogen-activated protein (MAP) kinases, but not JNK or p38, specifically phosphorylate STAT3 at serine 727 in response to growth factors. Serine 161-167 signal transducer and activator of transcription 3 Homo sapiens 152-157 9343414-7 1997 STAT3 serine phosphorylation, not its tyrosine phosphorylation, results in retarded mobility of the STAT3 protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Serine 6-12 signal transducer and activator of transcription 3 Homo sapiens 0-5 9343414-7 1997 STAT3 serine phosphorylation, not its tyrosine phosphorylation, results in retarded mobility of the STAT3 protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Serine 6-12 signal transducer and activator of transcription 3 Homo sapiens 100-105 9343414-8 1997 Importantly, serine 727 phosphorylation negatively modulates STAT3 tyrosine phosphorylation, which is required for dimer formation, nuclear translocation, and the DNA binding activity of this transcriptional regulator. Serine 13-19 signal transducer and activator of transcription 3 Homo sapiens 61-66 9343414-9 1997 Interestingly, the cytokine interleukin-6 also stimulates STAT3 serine phosphorylation, but in contrast to growth factors, this occurs by an ERK-independent process. Serine 64-70 signal transducer and activator of transcription 3 Homo sapiens 58-63 9322921-1 1997 We recently reported that insulin stimulation results in the serine phosphorylation of STAT3 (signal transducer and activator of transcription-3). Serine 61-67 signal transducer and activator of transcription 3 Homo sapiens 87-92 9322921-1 1997 We recently reported that insulin stimulation results in the serine phosphorylation of STAT3 (signal transducer and activator of transcription-3). Serine 61-67 signal transducer and activator of transcription 3 Homo sapiens 94-144 9322921-2 1997 In the present study, we identified serine 727 as the site of insulin-stimulated STAT3 serine phosphorylation. Serine 36-42 signal transducer and activator of transcription 3 Homo sapiens 81-86 9322921-2 1997 In the present study, we identified serine 727 as the site of insulin-stimulated STAT3 serine phosphorylation. Serine 87-93 signal transducer and activator of transcription 3 Homo sapiens 81-86 9322921-6 1997 In contrast, activation of the ERK MAP kinase pathway with both insulin and osmotic shock resulted in the serine phosphorylation of STAT3. Serine 106-112 signal transducer and activator of transcription 3 Homo sapiens 132-137 9322921-7 1997 In addition, expression of a dominant-interfering Ras mutant (N17Ras) or treatment with the specific MEK inhibitor (PD98059) prevented the insulin stimulation of STAT3 serine phosphorylation. Serine 168-174 signal transducer and activator of transcription 3 Homo sapiens 162-167 9322921-9 1997 Together, these data demonstrate that the insulin-stimulated serine phosphorylation of STAT3 occurs by a MEK-dependent pathway that is independent of ERK activation. Serine 61-67 signal transducer and activator of transcription 3 Homo sapiens 87-92 9182578-2 1997 For full transcriptional activation, Stat1 and Stat3 also require phosphorylation of a conserved serine residue within a mitogen-activated protein kinase phosphorylation consensus site. Serine 97-103 signal transducer and activator of transcription 3 Homo sapiens 47-52 9305919-9 1997 Hence, the present results show that STAT3 serine phosphorylation can be regulated independently of the tyrosine phosphorylation of this molecule. Serine 43-49 signal transducer and activator of transcription 3 Homo sapiens 37-42 8704168-4 1996 Simultaneous stimulation with SLF plus other cytokines that induce tyrosine phosphorylation of Stat3, such as interleukin-9 (IL-9) in MO7e cells or IL-6 in TF-1 cells, resulted in tyrosine phosphorylation and enhanced serine phosphorylation of Stat3. Serine 218-224 signal transducer and activator of transcription 3 Homo sapiens 95-100 8704168-0 1996 Steel factor induces serine phosphorylation of Stat3 in human growth factor-dependent myeloid cell lines. Serine 21-27 signal transducer and activator of transcription 3 Homo sapiens 47-52 9153303-0 1997 Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3. Serine 17-23 signal transducer and activator of transcription 3 Homo sapiens 11-16 9153303-2 1997 By examining phosphopeptide maps of wild-type and mutant protein we show here that the Stat3 serine phosphorylation, like the Stat1 serine phosphorylation, occurs on a single residue, serine 727. Serine 93-99 signal transducer and activator of transcription 3 Homo sapiens 87-92 9153303-2 1997 By examining phosphopeptide maps of wild-type and mutant protein we show here that the Stat3 serine phosphorylation, like the Stat1 serine phosphorylation, occurs on a single residue, serine 727. Serine 132-138 signal transducer and activator of transcription 3 Homo sapiens 87-92 9153303-2 1997 By examining phosphopeptide maps of wild-type and mutant protein we show here that the Stat3 serine phosphorylation, like the Stat1 serine phosphorylation, occurs on a single residue, serine 727. Serine 132-138 signal transducer and activator of transcription 3 Homo sapiens 87-92 9162009-1 1997 STAT (signal transducers and activators of transcription) proteins undergo cytokine-dependent phosphorylation on serine and tyrosine. Serine 113-119 signal transducer and activator of transcription 3 Homo sapiens 0-4 8704168-6 1996 However, costimulation with SLF plus IL-9 in MO7e cells resulted in the nuclear translocation of serine-hyperphosphorylated Stat3. Serine 97-103 signal transducer and activator of transcription 3 Homo sapiens 124-129 8704168-7 1996 Serine phosphorylation of Stat3 was also observed by the stimulation of cells with granulocyte-macrophage colony-stimulating factor and IL-3, which do not induce tyrosine phosphorylation of Stat3. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-31 8626489-5 1996 Furthermore, tyrosine-phosphorylated STAT3 bound to the IFNAR-1 chain also undergoes a secondary modification involving serine phosphorylation. Serine 120-126 signal transducer and activator of transcription 3 Homo sapiens 37-42 8647800-7 1996 Together, these data demonstrate that insulin is a specific activator of STAT3 serine phosphorylation without affecting the other STAT isoforms. Serine 79-85 signal transducer and activator of transcription 3 Homo sapiens 73-78 8647800-0 1996 Insulin stimulates the serine phosphorylation of the signal transducer and activator of transcription (STAT3) isoform. Serine 23-29 signal transducer and activator of transcription 3 Homo sapiens 103-108 7543024-0 1995 Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Serine 82-88 signal transducer and activator of transcription 3 Homo sapiens 49-54 7543024-3 1995 Both cytokines and growth factors are capable of inducing the serine phosphorylation of Stat1 and Stat3. Serine 62-68 signal transducer and activator of transcription 3 Homo sapiens 98-103 7701321-3 1995 Serine phosphorylation of Stat3 was constitutive and was enhanced by signaling through gp130. Serine 0-6 signal transducer and activator of transcription 3 Homo sapiens 26-31 7624343-3 1995 Here we show that all cytokines that utilize gp130 sequentially induce two distinct forms of STAT3 in all responding cells examined, with the two forms apparently differing because of a time-dependent secondary serine/threonine phosphorylation involving an H7-sensitive kinase. Serine 211-217 signal transducer and activator of transcription 3 Homo sapiens 93-98 7701321-4 1995 In cells of lymphoid and neuronal origins, inhibition of serine phosphorylation prevented the formation of complexes of DNA with Stat3-Stat3 but not with Stat3-Stat1 or Stat1-Stat1 dimers. Serine 57-63 signal transducer and activator of transcription 3 Homo sapiens 129-134 7701321-4 1995 In cells of lymphoid and neuronal origins, inhibition of serine phosphorylation prevented the formation of complexes of DNA with Stat3-Stat3 but not with Stat3-Stat1 or Stat1-Stat1 dimers. Serine 57-63 signal transducer and activator of transcription 3 Homo sapiens 135-140 7701321-4 1995 In cells of lymphoid and neuronal origins, inhibition of serine phosphorylation prevented the formation of complexes of DNA with Stat3-Stat3 but not with Stat3-Stat1 or Stat1-Stat1 dimers. Serine 57-63 signal transducer and activator of transcription 3 Homo sapiens 135-140 7701321-5 1995 In vitro serine dephosphorylation of Stat3 also inhibited DNA binding of Stat3-Stat3. Serine 9-15 signal transducer and activator of transcription 3 Homo sapiens 37-42 7701321-5 1995 In vitro serine dephosphorylation of Stat3 also inhibited DNA binding of Stat3-Stat3. Serine 9-15 signal transducer and activator of transcription 3 Homo sapiens 73-78 7701321-5 1995 In vitro serine dephosphorylation of Stat3 also inhibited DNA binding of Stat3-Stat3. Serine 9-15 signal transducer and activator of transcription 3 Homo sapiens 73-78 7701321-6 1995 The requirement of serine phosphorylation for Stat3-Stat3.DNA complex formation was inversely correlated with the affinity of Stat3-Stat3 for the binding site. Serine 19-25 signal transducer and activator of transcription 3 Homo sapiens 46-51 7701321-6 1995 The requirement of serine phosphorylation for Stat3-Stat3.DNA complex formation was inversely correlated with the affinity of Stat3-Stat3 for the binding site. Serine 19-25 signal transducer and activator of transcription 3 Homo sapiens 52-57 7701321-6 1995 The requirement of serine phosphorylation for Stat3-Stat3.DNA complex formation was inversely correlated with the affinity of Stat3-Stat3 for the binding site. Serine 19-25 signal transducer and activator of transcription 3 Homo sapiens 52-57 7701321-6 1995 The requirement of serine phosphorylation for Stat3-Stat3.DNA complex formation was inversely correlated with the affinity of Stat3-Stat3 for the binding site. Serine 19-25 signal transducer and activator of transcription 3 Homo sapiens 52-57 7701321-7 1995 Thus, serine phosphorylation appears to enhance or to be required for the formation of stable Stat3-Stat3.DNA complexes. Serine 6-12 signal transducer and activator of transcription 3 Homo sapiens 94-99 7701321-7 1995 Thus, serine phosphorylation appears to enhance or to be required for the formation of stable Stat3-Stat3.DNA complexes. Serine 6-12 signal transducer and activator of transcription 3 Homo sapiens 100-105 33772065-3 2021 Previously, we showed that nuclear expression of the signal transducer and activator of transcription 3 (STAT3), phosphorylated at its serine 727 (pS727), was inversely proportional to the overall survival of ccRCC patients. Serine 135-141 signal transducer and activator of transcription 3 Homo sapiens 53-103 7533107-0 1995 Interleukin-6-induced serine phosphorylation of transcription factor APRF: evidence for a role in interleukin-6 target gene induction. Serine 22-28 signal transducer and activator of transcription 3 Homo sapiens 69-73 7533107-4 1995 We now show that IL-6 triggers a delayed phosphorylation of APRF at serine resudues which can be reversed in vitro by protein phosphatase 2A and is also inhibited by H7. Serine 68-74 signal transducer and activator of transcription 3 Homo sapiens 60-64 7533107-5 1995 Therefore, APRF serine phosphorylation is likely to represent a crucial event in IL-6 signal transduction leading to target gene induction. Serine 16-22 signal transducer and activator of transcription 3 Homo sapiens 11-15 33972660-8 2021 Importantly, FMRP could promote the IL-6-mediated translation of STAT3, and serine 114 of FMRP is identified as a potential phosphorylation site required for IL-6-mediated STAT3 translation. Serine 76-82 signal transducer and activator of transcription 3 Homo sapiens 172-177 33772065-3 2021 Previously, we showed that nuclear expression of the signal transducer and activator of transcription 3 (STAT3), phosphorylated at its serine 727 (pS727), was inversely proportional to the overall survival of ccRCC patients. Serine 135-141 signal transducer and activator of transcription 3 Homo sapiens 105-110 34731785-5 2021 However, STAT3 phosphorylated at a single serine residue can allow incorporation of this protein into the inner mitochondrial membrane to support oxidative phosphorylation (OXPHOS) and maximize the utility of glucose sources. Serine 42-48 signal transducer and activator of transcription 3 Homo sapiens 9-14 34857889-0 2022 Oncogenic dependency on STAT3 serine phosphorylation in KRAS mutant lung cancer. Serine 30-36 signal transducer and activator of transcription 3 Homo sapiens 24-29 34699606-4 2021 The mechanism investigation showed that zinc induced activation of extracellular regulated protein kinases (ERK) and Janus kinase 2 (JAK2), which phosphorylated signal transduction and transcription activator 3 (Stat3) at serine 727 (S727-Stat3) and tyrosine 705 (Y705-Stat3) respectively, resulting in activation of Stat3. Serine 222-228 signal transducer and activator of transcription 3 Homo sapiens 161-210 34699606-4 2021 The mechanism investigation showed that zinc induced activation of extracellular regulated protein kinases (ERK) and Janus kinase 2 (JAK2), which phosphorylated signal transduction and transcription activator 3 (Stat3) at serine 727 (S727-Stat3) and tyrosine 705 (Y705-Stat3) respectively, resulting in activation of Stat3. Serine 222-228 signal transducer and activator of transcription 3 Homo sapiens 212-217 34699606-4 2021 The mechanism investigation showed that zinc induced activation of extracellular regulated protein kinases (ERK) and Janus kinase 2 (JAK2), which phosphorylated signal transduction and transcription activator 3 (Stat3) at serine 727 (S727-Stat3) and tyrosine 705 (Y705-Stat3) respectively, resulting in activation of Stat3. Serine 222-228 signal transducer and activator of transcription 3 Homo sapiens 239-244 34699606-4 2021 The mechanism investigation showed that zinc induced activation of extracellular regulated protein kinases (ERK) and Janus kinase 2 (JAK2), which phosphorylated signal transduction and transcription activator 3 (Stat3) at serine 727 (S727-Stat3) and tyrosine 705 (Y705-Stat3) respectively, resulting in activation of Stat3. Serine 222-228 signal transducer and activator of transcription 3 Homo sapiens 317-322 34857889-2 2022 By contrast, an alternate mitochondrial pool of serine phosphorylated (pS727 site) STAT3 has been shown to promote tumourigenesis by regulating metabolic processes, although this has been reported in only a restricted number of mutant RAS-addicted neoplasms. Serine 48-54 signal transducer and activator of transcription 3 Homo sapiens 83-88 34884773-0 2021 Opposing Effects of Chelidonine on Tyrosine and Serine Phosphorylation of STAT3 in Human Uveal Melanoma Cells. Serine 48-54 signal transducer and activator of transcription 3 Homo sapiens 74-79 34885140-6 2021 Sorafenib-adapted cells also exhibited increased serine 727 phosphorylated (pSer727) STAT3, the prevalent form in mitochondria, suggesting that STAT3 might be an actionable target to counteract resistance. Serine 49-55 signal transducer and activator of transcription 3 Homo sapiens 85-90 34884773-7 2021 According to our flow cytometry and confocal microscopy data, chelidonine abrogated IL-6-induced activation and nuclear translocation, but amplified constitutive serine phosphorylation of STAT3. Serine 162-168 signal transducer and activator of transcription 3 Homo sapiens 188-193 34884773-3 2021 STAT3 also contains a serine phosphorylation site, with a postulated regulatory role in STAT3 activation and G2/M transition. Serine 22-28 signal transducer and activator of transcription 3 Homo sapiens 0-5 34100606-7 2021 Chrysin suppressed pseudo-allergic reactions through the PLC/IP3/Ca2+ and ERK/STAT3 serine 727 pathways downstream of MrgX2. Serine 84-90 signal transducer and activator of transcription 3 Homo sapiens 78-83 34626364-13 2022 CONCLUSIONS: Our findings suggest that, instead of tyrosine phosphorylation, serine phosphorylation of STAT3 is commonly activated in the kidney of patients with COVID-19. Serine 77-83 signal transducer and activator of transcription 3 Homo sapiens 103-108 34440160-4 2021 In this review, we describe the studies performed on the main modifications affecting the activity of STAT3: phosphorylation of tyrosine 705 and serine 727; acetylation of lysine 49, 87, 601, 615, 631, 685, 707, and 709; and methylation of lysine 49, 140, and 180. Serine 145-151 signal transducer and activator of transcription 3 Homo sapiens 102-107