PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18754814-2 2009 The present study tested the hypothesis that NAADP induces Ca(2+) release from the lysosomal store via a TRP-ML1 (transient receptor potential-mucolipin 1)-mediated Ca(2+) release channel in coronary arterial myocytes (CAMs). NAADP 45-50 mucolipin TRP cation channel 1 Homo sapiens 105-112 22534131-1 2012 Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca(2+)-releasing second messenger that might regulate different ion channels, including the ryanodine receptor, two-pore channels, and TRP-ML1 (transient receptor potential channel, subtype mucolipin 1), a Ca(2+) channel localized to lysosomes. NAADP 0-45 mucolipin TRP cation channel 1 Homo sapiens 201-208 22534131-1 2012 Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca(2+)-releasing second messenger that might regulate different ion channels, including the ryanodine receptor, two-pore channels, and TRP-ML1 (transient receptor potential channel, subtype mucolipin 1), a Ca(2+) channel localized to lysosomes. NAADP 47-52 mucolipin TRP cation channel 1 Homo sapiens 201-208 21613607-2 2011 Given that lysosomal nicotinic acid adenine dinucleotide phosphate (NAADP)-Ca(2+) release channel activity is associated with TRP-ML1, the present study was designed to test the hypothesis that NAADP regulates lysosome function via activation of TRP-ML1 channel activity. NAADP 21-66 mucolipin TRP cation channel 1 Homo sapiens 126-133 21613607-2 2011 Given that lysosomal nicotinic acid adenine dinucleotide phosphate (NAADP)-Ca(2+) release channel activity is associated with TRP-ML1, the present study was designed to test the hypothesis that NAADP regulates lysosome function via activation of TRP-ML1 channel activity. NAADP 21-66 mucolipin TRP cation channel 1 Homo sapiens 246-253 21613607-2 2011 Given that lysosomal nicotinic acid adenine dinucleotide phosphate (NAADP)-Ca(2+) release channel activity is associated with TRP-ML1, the present study was designed to test the hypothesis that NAADP regulates lysosome function via activation of TRP-ML1 channel activity. NAADP 68-73 mucolipin TRP cation channel 1 Homo sapiens 126-133 21613607-2 2011 Given that lysosomal nicotinic acid adenine dinucleotide phosphate (NAADP)-Ca(2+) release channel activity is associated with TRP-ML1, the present study was designed to test the hypothesis that NAADP regulates lysosome function via activation of TRP-ML1 channel activity. NAADP 194-199 mucolipin TRP cation channel 1 Homo sapiens 126-133 21613607-2 2011 Given that lysosomal nicotinic acid adenine dinucleotide phosphate (NAADP)-Ca(2+) release channel activity is associated with TRP-ML1, the present study was designed to test the hypothesis that NAADP regulates lysosome function via activation of TRP-ML1 channel activity. NAADP 194-199 mucolipin TRP cation channel 1 Homo sapiens 246-253 21613607-3 2011 Using lysosomal preparations from wild-type (TRP-ML1(+/+)) human fibroblasts, channel reconstitution experiments demonstrated that NAADP (0.01-1.0 muM) produced a concentration-dependent increase in TRP-ML1 channel activity. NAADP 131-136 mucolipin TRP cation channel 1 Homo sapiens 45-52 21613607-3 2011 Using lysosomal preparations from wild-type (TRP-ML1(+/+)) human fibroblasts, channel reconstitution experiments demonstrated that NAADP (0.01-1.0 muM) produced a concentration-dependent increase in TRP-ML1 channel activity. NAADP 131-136 mucolipin TRP cation channel 1 Homo sapiens 199-206 21613607-4 2011 This NAADP-induced activation of TRP-ML1 channels could not be observed in lysosomes from TRP-ML1(-/-) cells, but was restored by introducing a TRP-ML1 transgene into these cells. NAADP 5-10 mucolipin TRP cation channel 1 Homo sapiens 33-40 21613607-4 2011 This NAADP-induced activation of TRP-ML1 channels could not be observed in lysosomes from TRP-ML1(-/-) cells, but was restored by introducing a TRP-ML1 transgene into these cells. NAADP 5-10 mucolipin TRP cation channel 1 Homo sapiens 90-97 21613607-4 2011 This NAADP-induced activation of TRP-ML1 channels could not be observed in lysosomes from TRP-ML1(-/-) cells, but was restored by introducing a TRP-ML1 transgene into these cells. NAADP 5-10 mucolipin TRP cation channel 1 Homo sapiens 90-97 21613607-5 2011 Microscopic Ca(2+) fluorescence imaging showed that NAADP significantly increased intracellular Ca(2+) concentration to 302.4 +- 74.28 nM (vs. 180 +- 44.13 nM of the basal) in TRP-ML1(+/+) cells, but it had no effect in TRP-ML1(-/-) cells. NAADP 52-57 mucolipin TRP cation channel 1 Homo sapiens 176-183 21613607-5 2011 Microscopic Ca(2+) fluorescence imaging showed that NAADP significantly increased intracellular Ca(2+) concentration to 302.4 +- 74.28 nM (vs. 180 +- 44.13 nM of the basal) in TRP-ML1(+/+) cells, but it had no effect in TRP-ML1(-/-) cells. NAADP 52-57 mucolipin TRP cation channel 1 Homo sapiens 220-227 21613607-6 2011 If a TRP-ML1 gene was transfected into TRP-ML1(-/-) cells, the Ca(2+) response to NAADP was restored to the level comparable to TRP-ML1(+/+) cells. NAADP 82-87 mucolipin TRP cation channel 1 Homo sapiens 5-12 21613607-6 2011 If a TRP-ML1 gene was transfected into TRP-ML1(-/-) cells, the Ca(2+) response to NAADP was restored to the level comparable to TRP-ML1(+/+) cells. NAADP 82-87 mucolipin TRP cation channel 1 Homo sapiens 39-46 21613607-6 2011 If a TRP-ML1 gene was transfected into TRP-ML1(-/-) cells, the Ca(2+) response to NAADP was restored to the level comparable to TRP-ML1(+/+) cells. NAADP 82-87 mucolipin TRP cation channel 1 Homo sapiens 39-46 21613607-7 2011 Functionally, confocal microscopy revealed that NAADP significantly enhanced the dynamic interaction of endosomes and lysosomes and the lipid delivery to lysosomes in TRP-ML1(+/+) cells. NAADP 48-53 mucolipin TRP cation channel 1 Homo sapiens 167-174 21613607-8 2011 This functional action of NAADP was abolished in TRP-ML1(-/-) cells, but restored after TRP-ML1 gene was rescued in these cells. NAADP 26-31 mucolipin TRP cation channel 1 Homo sapiens 49-56 21613607-8 2011 This functional action of NAADP was abolished in TRP-ML1(-/-) cells, but restored after TRP-ML1 gene was rescued in these cells. NAADP 26-31 mucolipin TRP cation channel 1 Homo sapiens 88-95 21613607-9 2011 Our results suggest that NAADP increases lysosomal TRP-ML1 channel activity to release Ca(2+), which promotes the interaction of endosomes and lysosomes and thereby regulates lipid transport to lysosomes. NAADP 25-30 mucolipin TRP cation channel 1 Homo sapiens 51-58 21540176-14 2011 We conclude that although TRPML1 and TPCs are present in the same complex, they function as two independent organellar ion channels and that TPCs, not TRPMLs, are the targets for NAADP. NAADP 179-184 mucolipin TRP cation channel 1 Homo sapiens 26-32 18754814-2 2009 The present study tested the hypothesis that NAADP induces Ca(2+) release from the lysosomal store via a TRP-ML1 (transient receptor potential-mucolipin 1)-mediated Ca(2+) release channel in coronary arterial myocytes (CAMs). NAADP 45-50 mucolipin TRP cation channel 1 Homo sapiens 114-154 18754814-8 2009 Immunoprecipitation or blockade of TRP-ML1 by anti-TRP-ML1 antibodies almost abolished NAADP-induced activation of lysosomal Ca(2+) channels (to 14.0 +/- 4.4% of control). NAADP 87-92 mucolipin TRP cation channel 1 Homo sapiens 35-42 18754814-8 2009 Immunoprecipitation or blockade of TRP-ML1 by anti-TRP-ML1 antibodies almost abolished NAADP-induced activation of lysosomal Ca(2+) channels (to 14.0 +/- 4.4% of control). NAADP 87-92 mucolipin TRP cation channel 1 Homo sapiens 51-58