PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32893576-1 2020 In this study, citrate synthase gene(CIT2), and malate synthase gene(MLS1) were successfully knocked out in beta-amyrin-producing yeast cells by using CRISPR/CAS9. beta-amyrin 108-119 malate synthase MLS1 Saccharomyces cerevisiae S288C 69-73 32893576-4 2020 Compared with the control strain, the production of beta-amyrin was increased by 1.85 times after deleting MLS1, reaching into 3.3 mg L~(-1). beta-amyrin 56-63 malate synthase MLS1 Saccharomyces cerevisiae S288C 107-111 32893576-6 2020 This study successfully knocked out the CITT2 and MLS1 genes and weakened the PGI1 gene by using CRISPR/CAS9, which directly influenced the production of beta-amyrin and provided some reference for the the metabolic engineering of triterpernoid producing strain. beta-amyrin 154-165 malate synthase MLS1 Saccharomyces cerevisiae S288C 50-54