PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 19153847-2 2009 The data show that cholesterol modulates electromotility mainly by influencing the motor protein prestin, less by affecting the passive membrane properties. Cholesterol 19-30 solute carrier family 26 member 5 Homo sapiens 97-104 23083705-0 2012 Membrane cholesterol strongly influences confined diffusion of prestin. Cholesterol 9-20 solute carrier family 26 member 5 Homo sapiens 63-70 23083705-2 2012 Prestin function is sensitive to membrane cholesterol levels, and numerous studies have suggested that prestin localizes in cholesterol-rich membrane microdomains. Cholesterol 42-53 solute carrier family 26 member 5 Homo sapiens 0-7 23083705-2 2012 Prestin function is sensitive to membrane cholesterol levels, and numerous studies have suggested that prestin localizes in cholesterol-rich membrane microdomains. Cholesterol 124-135 solute carrier family 26 member 5 Homo sapiens 103-110 23083705-3 2012 Previously, fluorescence recovery after photobleaching experiments were performed in HEK cells expressing prestin-GFP after cholesterol manipulations, and revealed evidence of transient confinement. Cholesterol 124-135 solute carrier family 26 member 5 Homo sapiens 106-113 23083705-8 2012 A complementary analysis of the distribution of squared displacements confirmed that cholesterol depletion reduces prestin confinement. Cholesterol 85-96 solute carrier family 26 member 5 Homo sapiens 115-122 23083705-9 2012 These findings support the hypothesis that prestin function is intimately linked to membrane organization, and further promote a regulatory role for cholesterol in OHC and auditory function. Cholesterol 149-160 solute carrier family 26 member 5 Homo sapiens 43-50 19898896-4 2010 Here, we show that the double mutant prestin(NN163/166AA) is not glycosylated and shows the expected NLC properties in the untreated and cholesterol-depleted HEK 293 cell model. Cholesterol 137-148 solute carrier family 26 member 5 Homo sapiens 37-44 19898896-7 2010 In an attempt to explain this finding, we discovered that both WT prestin and prestin(NN163/166AA) participate in cholesterol-dependent cellular trafficking. Cholesterol 114-125 solute carrier family 26 member 5 Homo sapiens 66-73 19898896-7 2010 In an attempt to explain this finding, we discovered that both WT prestin and prestin(NN163/166AA) participate in cholesterol-dependent cellular trafficking. Cholesterol 114-125 solute carrier family 26 member 5 Homo sapiens 78-85 19898896-8 2010 In contrast to WT prestin, prestin(NN163/166AA) shows a significant cholesterol-dependent decrease in cell-surface expression, which may explain the loss of NLC function. Cholesterol 68-79 solute carrier family 26 member 5 Homo sapiens 27-34 19898896-11 2010 We speculate that the cholesterol regulation of prestin occurs through localization to and internalization from membrane microdomains by clathrin- and caveolin-dependent mechanisms. Cholesterol 22-33 solute carrier family 26 member 5 Homo sapiens 48-55 19517190-2 2009 The importance of the plasma membrane environment in modulating OHC electromotility has been substantiated by recent studies demonstrating that membrane cholesterol alters prestin activity in a manner consistent with cholesterol-induced changes in auditory function. Cholesterol 153-164 solute carrier family 26 member 5 Homo sapiens 172-179 19517190-8 2009 These results support the idea that the cholesterol-dependent regulation of prestin function and electromotility correlates with changes in the properties of the lipid environment that surrounds and supports prestin. Cholesterol 40-51 solute carrier family 26 member 5 Homo sapiens 76-83 19517190-8 2009 These results support the idea that the cholesterol-dependent regulation of prestin function and electromotility correlates with changes in the properties of the lipid environment that surrounds and supports prestin. Cholesterol 40-51 solute carrier family 26 member 5 Homo sapiens 208-215 19153847-3 2009 OBJECTIVES: Elevated serum cholesterol is linked to inner ear disorders and may influence hearing by altering membrane properties of outer hair cells (OHCs) and by affecting the motor protein prestin. Cholesterol 27-38 solute carrier family 26 member 5 Homo sapiens 192-199 18567583-3 2008 We have previously shown that membrane cholesterol modulates the peak voltage of prestin-associated nonlinear capacitance in vivo and in vitro. Cholesterol 39-50 solute carrier family 26 member 5 Homo sapiens 81-88 18567583-4 2008 The present study explores the effects of membrane cholesterol and docosahexaenoic acid content on the peak and magnitude of prestin-associated charge movement in a human embryonic kidney (HEK 293) cell model. Cholesterol 51-62 solute carrier family 26 member 5 Homo sapiens 125-132 18567583-11 2008 Our results quantify the relation between membrane cholesterol concentration and prestin-associated charge movement and enhance our understanding of how membrane composition modulates prestin function. Cholesterol 51-62 solute carrier family 26 member 5 Homo sapiens 81-88 17321873-8 2007 Depleting membrane cholesterol content altered prestin localization and NLC. Cholesterol 19-30 solute carrier family 26 member 5 Homo sapiens 47-54 17933870-5 2007 To study the effects of cholesterol on hearing at the molecular level, we altered cholesterol levels in the OHC wall, which contains the membrane protein prestin. Cholesterol 82-93 solute carrier family 26 member 5 Homo sapiens 154-161 17933870-6 2007 We show a dynamic and reversible relationship between membrane cholesterol levels and voltage dependence of prestin-associated charge movement in both OHCs and prestin-transfected HEK 293 cells. Cholesterol 63-74 solute carrier family 26 member 5 Homo sapiens 108-115 17933870-6 2007 We show a dynamic and reversible relationship between membrane cholesterol levels and voltage dependence of prestin-associated charge movement in both OHCs and prestin-transfected HEK 293 cells. Cholesterol 63-74 solute carrier family 26 member 5 Homo sapiens 160-167 17933870-7 2007 Cholesterol levels also modulate the distribution of prestin within plasma membrane microdomains and affect prestin self-association in HEK 293 cells. Cholesterol 0-11 solute carrier family 26 member 5 Homo sapiens 53-60 17933870-7 2007 Cholesterol levels also modulate the distribution of prestin within plasma membrane microdomains and affect prestin self-association in HEK 293 cells. Cholesterol 0-11 solute carrier family 26 member 5 Homo sapiens 108-115 17933870-8 2007 These findings indicate that alterations in membrane cholesterol affect prestin function and functionally tune the outer hair cell. Cholesterol 53-64 solute carrier family 26 member 5 Homo sapiens 72-79