PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9869656-0 1999 Chinese hamster ovary cells overexpressing the oxysterol binding protein (OSBP) display enhanced synthesis of sphingomyelin in response to 25-hydroxycholesterol. 25-hydroxycholesterol 139-160 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 47-72 9869656-0 1999 Chinese hamster ovary cells overexpressing the oxysterol binding protein (OSBP) display enhanced synthesis of sphingomyelin in response to 25-hydroxycholesterol. 25-hydroxycholesterol 139-160 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 74-78 9869656-3 1999 The role of oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol, in activation of SM synthesis was assessed by overexpression in CHO-K1 cells. 25-hydroxycholesterol 75-96 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 12-37 9869656-3 1999 The role of oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol, in activation of SM synthesis was assessed by overexpression in CHO-K1 cells. 25-hydroxycholesterol 75-96 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 39-43 9869656-4 1999 When compared to mock transfected controls, three CHO-K1 clones overexpressing OSBP by 10- to 15-fold displayed a 2- to 3-fold enhancement of [3H]serine incorporation into sphingomyelin when treated with 25-hydroxycholesterol. 25-hydroxycholesterol 204-225 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 79-83 9869656-5 1999 Closer examination of one of these clones (CHO-OSBP cells) revealed a >8.5-fold stimulation of sphingomyelin synthesis after a 6-h treatment with 25-hydroxycholesterol compared to 3.5-fold in controls, slightly higher basal levels of sphingomyelin synthesis, and a more rapid response to 25-hydroxycholesterol. 25-hydroxycholesterol 149-170 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 47-51 9869656-5 1999 Closer examination of one of these clones (CHO-OSBP cells) revealed a >8.5-fold stimulation of sphingomyelin synthesis after a 6-h treatment with 25-hydroxycholesterol compared to 3.5-fold in controls, slightly higher basal levels of sphingomyelin synthesis, and a more rapid response to 25-hydroxycholesterol. 25-hydroxycholesterol 291-312 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 47-51 9869656-11 1999 Results from overexpressing cells show that OSBP potentiates the stimulatory effects of 25-hydroxycholesterol on sphingomyelin synthesis. 25-hydroxycholesterol 88-109 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 44-48 9869656-12 1999 25-Hydroxycholesterol promotes translocation of OSBP to the Golgi apparatus where it appears to stimulate conversion of ceramide to sphingomyelin. 25-hydroxycholesterol 0-21 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 48-52 9806908-0 1998 Cholesterol regulates oxysterol binding protein (OSBP) phosphorylation and Golgi localization in Chinese hamster ovary cells: correlation with stimulation of sphingomyelin synthesis by 25-hydroxycholesterol. 25-hydroxycholesterol 185-206 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 49-53 9487139-1 1998 Oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol that localizes to a Golgi/vesicular compartment, migrated on SDS-PAGE as a doublet of 96 and 101 kDa. 25-hydroxycholesterol 63-84 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 0-25 9487139-1 1998 Oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol that localizes to a Golgi/vesicular compartment, migrated on SDS-PAGE as a doublet of 96 and 101 kDa. 25-hydroxycholesterol 63-84 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 27-31 9337870-1 1997 Oxysterol-binding protein (OSBP) is a high-affinity receptor for a variety of oxysterols, such as 25-hydroxycholesterol, that down-regulate cholesterol synthesis and stimulate cholesterol esterification. 25-hydroxycholesterol 98-119 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 0-25 9337870-1 1997 Oxysterol-binding protein (OSBP) is a high-affinity receptor for a variety of oxysterols, such as 25-hydroxycholesterol, that down-regulate cholesterol synthesis and stimulate cholesterol esterification. 25-hydroxycholesterol 98-119 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 27-31 9337870-3 1997 Compared with mock-transfected controls, several cell lines overexpressing wild-type OSBP (CHO-OSBP) displayed a 50% decrease in cholesteryl ester synthesis when cultured in medium with delipidated serum, 25-hydroxycholesterol or low-density lipoprotein (LDL). 25-hydroxycholesterol 205-226 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 85-89 9337870-3 1997 Compared with mock-transfected controls, several cell lines overexpressing wild-type OSBP (CHO-OSBP) displayed a 50% decrease in cholesteryl ester synthesis when cultured in medium with delipidated serum, 25-hydroxycholesterol or low-density lipoprotein (LDL). 25-hydroxycholesterol 205-226 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 91-99 26601944-2 2016 25-Hydroxycholesterol (25OH) competitively inhibits this exchange reaction in vitro and causes the constitutive localization of OSBP at the ER/Golgi interface and PI-4P-dependent recruitment of ceramide transfer protein (CERT) for sphingomyelin synthesis. 25-hydroxycholesterol 0-21 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 128-132 26601944-2 2016 25-Hydroxycholesterol (25OH) competitively inhibits this exchange reaction in vitro and causes the constitutive localization of OSBP at the ER/Golgi interface and PI-4P-dependent recruitment of ceramide transfer protein (CERT) for sphingomyelin synthesis. 25-hydroxycholesterol 23-27 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 128-132 26601944-4 2016 Treatment of fibroblasts or Chinese hamster ovary (CHO) cells with 25OH caused a 50-70% reduction in Golgi-associated immunoreactive PI-4P that correlated with Golgi localization of OSBP. 25-hydroxycholesterol 67-71 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 182-186 26601944-5 2016 In contrast, 25OH caused an OSBP-dependent enrichment in Golgi PI-4P that was detected with a pleckstrin homology domain probe. 25-hydroxycholesterol 13-17 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 28-32 26601944-7 2016 The PI-4P and sterol binding activities of OSBP were both required for 25OH activation of sphingomyelin synthesis, suggesting that 25OH must be exchanged for PI-4P to be concentrated at contact sites. 25-hydroxycholesterol 71-75 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 43-47 26601944-7 2016 The PI-4P and sterol binding activities of OSBP were both required for 25OH activation of sphingomyelin synthesis, suggesting that 25OH must be exchanged for PI-4P to be concentrated at contact sites. 25-hydroxycholesterol 131-135 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 43-47 26601944-8 2016 We propose a model wherein 25OH activation of OSBP promotes the binding and retention of PI-4P at ER-Golgi contact sites. 25-hydroxycholesterol 27-31 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 46-50 18450749-9 2008 Sphingomyelin synthesis was suppressed in OSBP-depleted cells treated with 25-hydroxycholesterol but not TO901317 or 22-hydroxycholesterol and did not correlate with ABCA1 stabilization. 25-hydroxycholesterol 75-96 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 42-46 16571669-4 2006 RNA interference (RNAi) experiments in Chinese hamster ovary (CHO)-K1 cells revealed that OSBP and vesicle-associated membrane protein-associated protein (VAP) were required for stimulation of CERT-dependent ceramide transport and SM synthesis by 25-hydroxycholesterol and cholesterol depletion in response to cyclodextrin. 25-hydroxycholesterol 247-268 LOW QUALITY PROTEIN: oxysterol-binding protein 1 Cricetulus griseus 90-94