PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 12149270-5 2002 Both deoxycholic acid and lithocholic acid as well as CDCA, but not ursodeoxycholic acid, increase the mRNA level for the LDL receptor, even when Hep G2 cells are cultured with 25-hydroxycholesterol, a potent suppressor of gene expression for the LDL receptor. 25-hydroxycholesterol 177-198 low density lipoprotein receptor Homo sapiens 122-134 17156886-3 2007 METHODS: Expression and activity of LDLR were assessed by RT-PCR and LDL entry, in the absence or presence of squalestatin or 25-hydroxycholesterol that up- or down-regulates LDLR expression, respectively. 25-hydroxycholesterol 126-147 low density lipoprotein receptor Homo sapiens 175-179 17156886-8 2007 In hepatocytes pre-treated with squalestatin or 25-hydroxycholesterol before infection, viral RNA accumulation increased or decreased in parallel with LDLR mRNA expression and LDL entry. 25-hydroxycholesterol 48-69 low density lipoprotein receptor Homo sapiens 151-155 14704295-4 2004 Incubation of HepG2 cells in serum-free medium supplemented with 25-hydroxycholesterol (25OH, 5 mg/L) for 24 h decreased LDLr protein and mRNA by 50-70% (P<0.05) and mature SREBP-1 by 20-40% (P<0.05). 25-hydroxycholesterol 65-86 low density lipoprotein receptor Homo sapiens 121-125 14704295-4 2004 Incubation of HepG2 cells in serum-free medium supplemented with 25-hydroxycholesterol (25OH, 5 mg/L) for 24 h decreased LDLr protein and mRNA by 50-70% (P<0.05) and mature SREBP-1 by 20-40% (P<0.05). 25-hydroxycholesterol 88-92 low density lipoprotein receptor Homo sapiens 121-125 21168770-6 2010 Promoter-targeted duplex RNAs can overcome repression of LDLR expression by 25-hydroxycholesterol and do not interfere with activation of LDLR expression by lovastatin. 25-hydroxycholesterol 76-97 low density lipoprotein receptor Homo sapiens 57-61 19179626-5 2009 Cells treated with 25-hydroxycholesterol (50 microM) also displayed significant reduction in PCSK9 gene (37%, P<0.01) and protein (75% P<0.001) expression, whereas LDLr showed a decrease at the gene (30%, P<0.05) and protein (57%, P<0.01) levels, respectively. 25-hydroxycholesterol 19-40 low density lipoprotein receptor Homo sapiens 170-174 16251608-3 2005 Incubation of fibroblasts and HepG2 cells in serum-free medium supplemented with 25-hydroxycholesterol (25OH-cholesterol, 5 mg/L) for 24 h decreased LDLr protein and mRNA levels by 50-90% (P < 0.05). 25-hydroxycholesterol 81-102 low density lipoprotein receptor Homo sapiens 149-153 12149270-5 2002 Both deoxycholic acid and lithocholic acid as well as CDCA, but not ursodeoxycholic acid, increase the mRNA level for the LDL receptor, even when Hep G2 cells are cultured with 25-hydroxycholesterol, a potent suppressor of gene expression for the LDL receptor. 25-hydroxycholesterol 177-198 low density lipoprotein receptor Homo sapiens 247-259 10333389-10 1999 The transcriptional regulator 25-hydroxycholesterol suppresses both HMGR and LDLR activities, while the post-transcriptional regulatory lanosterol analogs exhibit a more desirable profile, lowering HMGR levels without suppressing LDLR expression, and in some cases actually enhancing cellular LDL metabolism. 25-hydroxycholesterol 30-51 low density lipoprotein receptor Homo sapiens 77-81 8057282-4 1994 In the same system, the related oxysterol, 25-hydroxycholesterol (1), at 10 microM lowered both HMGR mRNA levels and LDL-receptor activity by 58% and 64%, respectively. 25-hydroxycholesterol 43-64 low density lipoprotein receptor Homo sapiens 117-129 8576635-5 1995 In contrast, incubation of the cells with 25-hydroxycholesterol produced a 30% decrease of LDL receptor expression. 25-hydroxycholesterol 42-63 low density lipoprotein receptor Homo sapiens 91-103 9351383-8 1997 In contrast, no significant effect on the level of mRNA for the LDL receptor was observed after incubation with cafestol, whereas 25-hydroxycholesterol reduced the mRNA level for the LDL receptor by 40% to 50% (P < .05). 25-hydroxycholesterol 130-151 low density lipoprotein receptor Homo sapiens 183-195 9034205-8 1997 To determine whether the increase in transcriptional activity of the LDL-R gene was secondary to changes in the cholesterol regulatory pool we performed experiments in which the cell cholesterol content was modified by the addition of either 25-hydroxycholesterol and mevalonate or inhibitors of ACAT activity (SA-58035 and progesterone). 25-hydroxycholesterol 242-263 low density lipoprotein receptor Homo sapiens 69-74 7595067-0 1995 Regulation of low density lipoprotein receptor gene expression in HepG2 and Caco2 cells by palmitate, oleate, and 25-hydroxycholesterol. 25-hydroxycholesterol 114-135 low density lipoprotein receptor Homo sapiens 14-46 7595067-8 1995 The suppressive effect of 25-hydroxycholesterol on LDL-receptor regulation was studied by incubating HepG2 and Caco2 cells grown either in 10% FCS or 10% LPDS for 24 h and then for 0-24 h with various doses of 25-hydroxycholesterol. 25-hydroxycholesterol 26-47 low density lipoprotein receptor Homo sapiens 51-63 7595067-14 1995 The combination of palmitate (0.8 mM) and 25-hydroxycholesterol (2.5-5 micrograms/ml) suppressed LDL-receptor activity by 65% in HepG2 cells and by 52% in Caco2 cells. 25-hydroxycholesterol 42-63 low density lipoprotein receptor Homo sapiens 97-109 8305487-0 1994 Ketoconazole and 25-hydroxycholesterol produce reciprocal changes in the rate of transcription of the human LDL receptor gene. 25-hydroxycholesterol 17-38 low density lipoprotein receptor Homo sapiens 108-120 8305487-5 1994 Ketoconazole- and 25-hydroxycholesterol-induced changes in LDL receptor mRNA accumulation were due to alterations in the relative rate of LDL receptor gene transcription as measured by nuclear run-on transcription. 25-hydroxycholesterol 18-39 low density lipoprotein receptor Homo sapiens 59-71 8305487-5 1994 Ketoconazole- and 25-hydroxycholesterol-induced changes in LDL receptor mRNA accumulation were due to alterations in the relative rate of LDL receptor gene transcription as measured by nuclear run-on transcription. 25-hydroxycholesterol 18-39 low density lipoprotein receptor Homo sapiens 138-150 8388388-7 1993 In contrast, 25-hydroxycholesterol strongly cosuppressed HMG-CoA reductase protein and mRNA levels and the low density lipoprotein receptor protein. 25-hydroxycholesterol 13-34 low density lipoprotein receptor Homo sapiens 107-139 8387332-8 1993 As assessed by immunoblots and steady-state labeling of proteins followed by immunoprecipitation of the LDL receptor, cells incubated with micellar 25-hydroxycholesterol contained substantially less receptor protein. 25-hydroxycholesterol 148-169 low density lipoprotein receptor Homo sapiens 104-116 2471639-3 1989 The content of LDL-receptor mRNA was reduced when the cells were pre-incubated in medium containing foetal calf serum, 25-hydroxycholesterol, or LDL, compared to that measured in cells which had been pre-incubated in medium containing lipoprotein-deficient serum (LPDS). 25-hydroxycholesterol 119-140 low density lipoprotein receptor Homo sapiens 15-27 2086705-10 1990 By contrast, the oxygenated sterol, 25-hydroxycholesterol, and mevalonate, the precursor of endogenously synthesized sterols, down-regulated LDL receptor mRNA levels comparably in mitogen-stimulated and control PBMC. 25-hydroxycholesterol 36-57 low density lipoprotein receptor Homo sapiens 141-153 2688859-3 1989 LDL and 25-hydroxycholesterol reduce LDL receptor expression, but this suppressive effect is partially overcome by 8-bromo-cAMP. 25-hydroxycholesterol 8-29 low density lipoprotein receptor Homo sapiens 37-49 1716067-7 1991 Mediation of binding by the LDL receptor is demonstrated by correspondence between the LDL receptor dissociation constant derived from this work and literature values; increased specific binding in lymphocytes cultured in lipoprotein-deficient media to up-regulate the LDL receptor; and decreased specific binding in lymphocytes cultured in the presence of 25-hydroxy cholesterol for 48 h to suppress the LDL receptor. 25-hydroxycholesterol 357-379 low density lipoprotein receptor Homo sapiens 28-40 2023060-3 1991 Some cholesterol oxides, notably cholestane-3 beta, 5 alpha, 6 beta-triol and 25-hydroxycholesterol, have been shown to cause injury to vascular endothelial and smooth muscle cells, to alter LDL receptor function, to enhance cholesteryl ester accumulation, to inhibit prostacyclin production, and to induce experimental atherosclerosis alone or in combination with cholesterol. 25-hydroxycholesterol 78-99 low density lipoprotein receptor Homo sapiens 191-203 2180958-4 1990 Coincubations with 25-hydroxycholesterol markedly attenuated fibroblast LDL receptor protein and mRNA response to growth activation. 25-hydroxycholesterol 19-40 low density lipoprotein receptor Homo sapiens 72-84 3320557-4 1987 The tropic hormone effects on LDL receptor mRNA are observed even in the presence of 25-hydroxycholesterol and aminoglutethimide, which by themselves suppress LDL receptor mRNA. 25-hydroxycholesterol 85-106 low density lipoprotein receptor Homo sapiens 30-42 3794551-9 1986 Although 25-hydroxycholesterol induced a decrease in LDL receptor content and synthesis within 6 hr, this action was overridden by simultaneous exposure to hCG. 25-hydroxycholesterol 9-30 low density lipoprotein receptor Homo sapiens 53-65 3241124-1 1988 Treatment of cultured human skin fibroblasts with cycloheximide retarded the down-regulation of low density lipoprotein (LDL) receptor activity caused by 25-hydroxycholesterol. 25-hydroxycholesterol 154-175 low density lipoprotein receptor Homo sapiens 96-134 3390448-5 1988 25-Hydroxycholesterol in the presence of aminoglutethimide suppressed low-density lipoprotein (LDL) receptor mRNA, but 8-bromo-cAMP effected a significant stimulation of LDL receptor mRNA levels when added with hydroxysterol and aminoglutethimide. 25-hydroxycholesterol 0-21 low density lipoprotein receptor Homo sapiens 70-108 2840565-2 1987 After 1 h of treatment with 8-bromo-cAMP, an appreciable increase in hybridizable LDL receptor mRNA was found which increased to apparently maximal levels within 4-6 h. Treatment of the granulosa cells with 25-hydroxycholesterol, in the presence of aminoglutethimide, resulted in a reduction in LDL receptor mRNA content within 6 h of treatment. 25-hydroxycholesterol 207-228 low density lipoprotein receptor Homo sapiens 82-94 2840565-2 1987 After 1 h of treatment with 8-bromo-cAMP, an appreciable increase in hybridizable LDL receptor mRNA was found which increased to apparently maximal levels within 4-6 h. Treatment of the granulosa cells with 25-hydroxycholesterol, in the presence of aminoglutethimide, resulted in a reduction in LDL receptor mRNA content within 6 h of treatment. 25-hydroxycholesterol 207-228 low density lipoprotein receptor Homo sapiens 295-307 3320557-4 1987 The tropic hormone effects on LDL receptor mRNA are observed even in the presence of 25-hydroxycholesterol and aminoglutethimide, which by themselves suppress LDL receptor mRNA. 25-hydroxycholesterol 85-106 low density lipoprotein receptor Homo sapiens 159-171 3949765-7 1986 Compound 58-035 also increased the down-regulation of the J774 LDL receptor in the presence of 25-hydroxycholesterol and acetyl-LDL but not in the presence of cholesteryl hemisuccinate, which is not an ACAT substrate. 25-hydroxycholesterol 95-116 low density lipoprotein receptor Homo sapiens 63-75 3004340-6 1986 25-Hydroxycholesterol, which enters cells without the requirement of low density lipoprotein-receptor binding, inhibited the HMG-CoA reductase activity in familial hypercholesterolemic cells by reversible phosphorylation. 25-hydroxycholesterol 0-21 low density lipoprotein receptor Homo sapiens 69-101 4055779-6 1985 A combination of 25-hydroxycholesterol and aminoglutethimide resulted in a 60% suppression of label incorporation into mature LDL receptor compared to untreated cells. 25-hydroxycholesterol 17-38 low density lipoprotein receptor Homo sapiens 126-138 2991417-5 1985 The glucocorticoid-induced enhancement of LDL receptor and cholesterol synthesis is abolished by preincubation of the cells with dexamethasone in combination with 25-hydroxycholesterol. 25-hydroxycholesterol 163-184 low density lipoprotein receptor Homo sapiens 42-54 7356845-3 1980 In normal fibroblasts preincubated in lipoprotein-deficient serum, LDL or 25-hydroxycholesterol decreased cholesterol synthesis and LDL receptor activity and increased cholesterol ester formation. 25-hydroxycholesterol 74-95 low density lipoprotein receptor Homo sapiens 132-144