PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10467260-0 1999 Overexpression of Akt (protein kinase B) confers protection against apoptosis and prevents formation of ceramide in response to pro-apoptotic stimuli. Ceramides 104-112 AKT serine/threonine kinase 1 Homo sapiens 18-21 10467260-6 1999 Both neo-transfected and the c-Akt dominant-negative transfected F-11 cells showed increased ceramide formation (twofold) in response to staurosporine, wortmannin, or LY294002; whereas cells with a constitutively active Akt (Myr-Akt) showed no increase in ceramide when treated with staurosporine, wortmannin, or LY294002. Ceramides 93-101 AKT serine/threonine kinase 1 Homo sapiens 31-34 10467260-6 1999 Both neo-transfected and the c-Akt dominant-negative transfected F-11 cells showed increased ceramide formation (twofold) in response to staurosporine, wortmannin, or LY294002; whereas cells with a constitutively active Akt (Myr-Akt) showed no increase in ceramide when treated with staurosporine, wortmannin, or LY294002. Ceramides 256-264 AKT serine/threonine kinase 1 Homo sapiens 31-34 10467260-8 1999 Overexpression of PI3K (p110) and Akt protected cells against ceramide-induced apoptosis, suggesting that Ceramide action is upstream of Akt in these cells and suggesting that Akt might be a target for inhibition by ceramide. Ceramides 62-70 AKT serine/threonine kinase 1 Homo sapiens 34-37 10467260-8 1999 Overexpression of PI3K (p110) and Akt protected cells against ceramide-induced apoptosis, suggesting that Ceramide action is upstream of Akt in these cells and suggesting that Akt might be a target for inhibition by ceramide. Ceramides 62-70 AKT serine/threonine kinase 1 Homo sapiens 137-140 10467260-8 1999 Overexpression of PI3K (p110) and Akt protected cells against ceramide-induced apoptosis, suggesting that Ceramide action is upstream of Akt in these cells and suggesting that Akt might be a target for inhibition by ceramide. Ceramides 62-70 AKT serine/threonine kinase 1 Homo sapiens 137-140 10467260-8 1999 Overexpression of PI3K (p110) and Akt protected cells against ceramide-induced apoptosis, suggesting that Ceramide action is upstream of Akt in these cells and suggesting that Akt might be a target for inhibition by ceramide. Ceramides 106-114 AKT serine/threonine kinase 1 Homo sapiens 34-37 10467260-8 1999 Overexpression of PI3K (p110) and Akt protected cells against ceramide-induced apoptosis, suggesting that Ceramide action is upstream of Akt in these cells and suggesting that Akt might be a target for inhibition by ceramide. Ceramides 106-114 AKT serine/threonine kinase 1 Homo sapiens 137-140 10467260-8 1999 Overexpression of PI3K (p110) and Akt protected cells against ceramide-induced apoptosis, suggesting that Ceramide action is upstream of Akt in these cells and suggesting that Akt might be a target for inhibition by ceramide. Ceramides 106-114 AKT serine/threonine kinase 1 Homo sapiens 137-140 10467260-8 1999 Overexpression of PI3K (p110) and Akt protected cells against ceramide-induced apoptosis, suggesting that Ceramide action is upstream of Akt in these cells and suggesting that Akt might be a target for inhibition by ceramide. Ceramides 216-224 AKT serine/threonine kinase 1 Homo sapiens 34-37 9710629-0 1998 Regulation of insulin-stimulated glucose transporter GLUT4 translocation and Akt kinase activity by ceramide. Ceramides 100-108 AKT serine/threonine kinase 1 Homo sapiens 77-80 8757935-4 1996 Upstream inputs are diverse, and include small GTPases (primarily Rac and Cdc42; secondarily Ras) acting through mammalian homologs of the yeast Ste20 kinase, other kinase subfamilies (e.g. GC kinase) and ceramide, a putative second messenger for certain TNF-alpha actions. Ceramides 205-213 AKT serine/threonine kinase 1 Homo sapiens 66-69 9710629-9 1998 These studies demonstrate ceramide"s capacity to inhibit activation of Akt and imply that this is a mechanism of antagonism of insulin-dependent physiological events, such as the peripheral activation of glucose transport and the suppression of apoptosis. Ceramides 26-34 AKT serine/threonine kinase 1 Homo sapiens 71-74 9649498-5 1998 Down-regulation of PI(3)K by ceramide results in inhibition of the kinase Akt and decreased phosphorylation of the death effector Bad. Ceramides 29-37 AKT serine/threonine kinase 1 Homo sapiens 74-77 27852416-6 2016 Conclusion: Itraconazole induces apoptosis of PC-3 cell through increasing the intracellular ceramide content, which might relate to upregulation of cleavage-caspase 3 and Bax, downregulation of Bcl-2 and inactivation of Akt-mTORC signal pathway. Ceramides 93-101 AKT serine/threonine kinase 1 Homo sapiens 221-224 33003449-0 2020 Ceramide-Enriched Membrane Domains Contribute to Targeted and Nontargeted Effects of Radiation through Modulation of PI3K/AKT Signaling in HNSCC Cells. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 122-125 29071793-6 2017 Overfeeding resulted in inhibition of Akt activity, which correlated with the reductions in smaller, peripherally located lipid droplets and drastic increases in ceramide content. Ceramides 162-170 AKT serine/threonine kinase 1 Homo sapiens 38-41 32455838-7 2020 Ceramides are sphingolipids with variable acyl group chain length and activate protein phosphatase 2A that dephosphorylates Akt to block insulin signaling. Ceramides 0-9 AKT serine/threonine kinase 1 Homo sapiens 124-127 29470962-7 2018 Results suggested that the ceramide/PP2A/Akt axis is involved in the apoptosis and a possible cyclooxygenase-independent target for indomethacin. Ceramides 27-35 AKT serine/threonine kinase 1 Homo sapiens 41-44 26201515-5 2015 Furthermore, the phosphatase-resistant Akt was refractory to ceramide-dependent dephosphorylation and amplified insulin-dependent Thr(308) phosphorylation in a regulated fashion. Ceramides 61-69 AKT serine/threonine kinase 1 Homo sapiens 39-42 26897748-7 2016 Signaling studies showed that AZD-8055 and C6 ceramide synergistically suppressed Akt-mTOR complex 1/2 cascade activation. Ceramides 46-54 AKT serine/threonine kinase 1 Homo sapiens 82-85 26698173-2 2016 However, in vitro treatments with saturated fatty acids or their derivative ceramide demonstrate an effect only at the Akt step. Ceramides 76-84 AKT serine/threonine kinase 1 Homo sapiens 119-122 26698173-6 2016 Short-term treatments of myotubes with palmitate, a ceramide precursor, or directly with ceramide induce an inhibition of Akt, whereas prolonged periods of treatment show an additive inhibition of insulin signaling through increased IRS1 serine 307 phosphorylation. Ceramides 52-60 AKT serine/threonine kinase 1 Homo sapiens 122-125 26698173-6 2016 Short-term treatments of myotubes with palmitate, a ceramide precursor, or directly with ceramide induce an inhibition of Akt, whereas prolonged periods of treatment show an additive inhibition of insulin signaling through increased IRS1 serine 307 phosphorylation. Ceramides 89-97 AKT serine/threonine kinase 1 Homo sapiens 122-125 26698173-10 2016 Together, in the long term, our results show that ceramide acts at two distinct levels of the insulin signaling pathway (IRS1 and Akt). Ceramides 50-58 AKT serine/threonine kinase 1 Homo sapiens 130-133 26446703-5 2015 Furthermore, in silico mutation studies and experimental validations led to a novel finding that concurrently targeting ceramide and PI3K/AKT pathway by chemical probes or marketed drugs achieves synergistic anti-cancer effects. Ceramides 120-128 AKT serine/threonine kinase 1 Homo sapiens 138-141 25576381-5 2015 Significantly, C6 ceramide plus docetaxel caused dramatic human epidermal growth factor receptor (HER)-1/-2 degradation and downstream Akt/Erk inhibition in HER-2 expressing MDA-231 cells. Ceramides 18-26 AKT serine/threonine kinase 1 Homo sapiens 135-138 26379195-9 2015 SGK-1 and AKT-1 two highly homologous kinases differently reacted to ceramide treatment, since SGK-1 increases in response to apoptotic stimulus while AKT-1 decreases. Ceramides 69-77 AKT serine/threonine kinase 1 Homo sapiens 10-15 26379195-9 2015 SGK-1 and AKT-1 two highly homologous kinases differently reacted to ceramide treatment, since SGK-1 increases in response to apoptotic stimulus while AKT-1 decreases. Ceramides 69-77 AKT serine/threonine kinase 1 Homo sapiens 151-156 26115843-4 2015 We found that both ceramide analogs D- and L-PDMP (1-phenyl 2-decanoylamino-3-morpholino-1-propanol), which have opposite effects on ganglioside synthesis, selectively inhibited GSK3beta via Ser9 phosphorylation independently of the upstream insulin/Akt pathway. Ceramides 19-27 AKT serine/threonine kinase 1 Homo sapiens 250-253 25058613-3 2014 The main aim of this study was to explore the mechanisms by which ceramide inhibits PKB/Akt in three different skeletal muscle-derived cell culture models; rat L6 myotubes, mouse C2C12 myotubes and primary human skeletal muscle cells. Ceramides 66-74 AKT serine/threonine kinase 1 Homo sapiens 84-91 26090071-6 2015 Ceramides may initiate a cascade of biochemical alterations, which ultimately leads to neuronal death by diverse mechanisms, including depolarization and permeabilization of mitochondria, increased production of reactive oxygen species (ROS), cytochrome c release, Bcl-2 depletion, and caspase-3 activation, mainly by modulating intracellular signalling, particularly along the pathways related to Akt/PKB kinase and mitogen-activated protein kinases (MAPKs). Ceramides 0-9 AKT serine/threonine kinase 1 Homo sapiens 398-401 26090071-6 2015 Ceramides may initiate a cascade of biochemical alterations, which ultimately leads to neuronal death by diverse mechanisms, including depolarization and permeabilization of mitochondria, increased production of reactive oxygen species (ROS), cytochrome c release, Bcl-2 depletion, and caspase-3 activation, mainly by modulating intracellular signalling, particularly along the pathways related to Akt/PKB kinase and mitogen-activated protein kinases (MAPKs). Ceramides 0-9 AKT serine/threonine kinase 1 Homo sapiens 402-405 25380816-0 2015 Akt/eNOS signaling pathway mediates inhibition of endothelial progenitor cells by palmitate-induced ceramide. Ceramides 100-108 AKT serine/threonine kinase 1 Homo sapiens 0-3 25152399-6 2014 By using pharmacological and siRNA-knockdown strategies, we showed that Akt-mammalian TOR (mTOR) over-activation was an important pemetrexed resistance factor in OS cells, and C6 ceramide-mediated pemetrexed sensitization effect was mediated, at least in part, by Akt-mTOR inhibition. Ceramides 179-187 AKT serine/threonine kinase 1 Homo sapiens 72-75 25152399-6 2014 By using pharmacological and siRNA-knockdown strategies, we showed that Akt-mammalian TOR (mTOR) over-activation was an important pemetrexed resistance factor in OS cells, and C6 ceramide-mediated pemetrexed sensitization effect was mediated, at least in part, by Akt-mTOR inhibition. Ceramides 179-187 AKT serine/threonine kinase 1 Homo sapiens 264-267 25152399-9 2014 Together, we conclude that C6 ceramide sensitizes pemetrexed-induced apoptosis and cytotoxicity in OS cells probably through in-activation of Akt-mTOR signaling. Ceramides 30-38 AKT serine/threonine kinase 1 Homo sapiens 142-145 24420784-4 2014 Our study indicated that ceramide significantly enhanced the level of free radicals and decreased the viability of the human neuroblastoma cell line (SH-SY5Y) through inhibition of the prosurvival PI3-K/Akt pathway. Ceramides 25-33 AKT serine/threonine kinase 1 Homo sapiens 203-206 25058613-4 2014 Our findings indicate that the mechanism by which ceramide acts to repress PKB/Akt is related to the myocellular abundance of caveolin-enriched domains (CEM) present at the plasma membrane. Ceramides 50-58 AKT serine/threonine kinase 1 Homo sapiens 75-82 25058613-5 2014 Here, we show that ceramide-enriched-CEMs are markedly more abundant in L6 myotubes compared to C2C12 myotubes, consistent with their previously reported role in coordinating aPKC-directed repression of PKB/Akt in L6 muscle cells. Ceramides 19-27 AKT serine/threonine kinase 1 Homo sapiens 203-210 25058613-6 2014 In contrast, a PP2A-dependent pathway predominantly mediates ceramide-induced inhibition of PKB/Akt in C2C12 myotubes. Ceramides 61-69 AKT serine/threonine kinase 1 Homo sapiens 92-99 25058613-7 2014 In addition, we demonstrate for the first time that ceramide engages an aPKC-dependent pathway to suppress insulin-induced PKB/Akt activation in palmitate-treated cultured human muscle cells as well as in muscle cells from diabetic patients. Ceramides 52-60 AKT serine/threonine kinase 1 Homo sapiens 123-130 24650522-0 2014 Ceramide inhibits insulin-stimulated Akt phosphorylation through activation of Rheb/mTORC1/S6K signaling in skeletal muscle. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 37-40 23019415-7 2012 ERK1/2 and Akt phosphorylation were significantly decreased after metronomic ceramide analog treatment. Ceramides 77-85 AKT serine/threonine kinase 1 Homo sapiens 11-14 24079644-6 2014 In these cells, caspase-3 was inhibited by ceramide-induced Akt phosphorylation, and by the induction of autophagic microtubule-associated protein-1 light-chain 3 lipidation. Ceramides 43-51 AKT serine/threonine kinase 1 Homo sapiens 60-63 24079644-9 2014 In cells from smokers, the prominent up-regulation of Akt pathways inhibited ceramide-triggered apoptosis, and was associated with elevated sphingosine and high-mobility group box 1, skewing the cell"s response toward autophagy and survival. Ceramides 77-85 AKT serine/threonine kinase 1 Homo sapiens 54-57 24079644-10 2014 In conclusion, the cell responses to ceramide are modulated by an intricate cross-talk between Akt signaling and sphingolipid metabolites, and profoundly modified by previous cigarette smoke exposure, which selects for an apoptosis-resistant phenotype. Ceramides 37-45 AKT serine/threonine kinase 1 Homo sapiens 95-98 24269881-4 2013 RESULTS: Ceramide treatment caused a dephosphorylation (p<0.05) of Akt and 4E-BP1, regardless of the presence of insulin. Ceramides 9-17 AKT serine/threonine kinase 1 Homo sapiens 70-84 23457308-0 2013 Ceramide mediates inhibition of the Akt/eNOS pathway by high levels of glucose in human vascular endothelial cells. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 36-39 23457308-1 2013 OBJECTIVE: To investigate how ceramide mediates the effects of high-glucose-induced inhibition of the Akt/endothelial nitric oxide synthase (eNOS) signalling pathway in human vascular endothelial cells (HUVECs). Ceramides 30-38 AKT serine/threonine kinase 1 Homo sapiens 102-105 23457308-7 2013 Preventing de novo ceramide synthesis attenuated the antagonistic effects of high-glucose levels on the Akt/eNOS signalling pathway (p<0.05); conversely, inducing ceramide build-up augmented the inhibitory effects of high-glucose levels on the Akt/eNOS signalling pathway (p<0.05). Ceramides 19-27 AKT serine/threonine kinase 1 Homo sapiens 104-107 23457308-7 2013 Preventing de novo ceramide synthesis attenuated the antagonistic effects of high-glucose levels on the Akt/eNOS signalling pathway (p<0.05); conversely, inducing ceramide build-up augmented the inhibitory effects of high-glucose levels on the Akt/eNOS signalling pathway (p<0.05). Ceramides 19-27 AKT serine/threonine kinase 1 Homo sapiens 247-250 23457308-8 2013 CONCLUSION: Ceramide is both necessary and sufficient for mediating the inhibition of the Akt/eNOS signalling pathway by high-glucose levels in endothelial cells. Ceramides 12-20 AKT serine/threonine kinase 1 Homo sapiens 90-93 24073738-7 2013 Ceramide treatment also increased H2O2 levels and reduced Akt/PKB (protein kinase B) phosphorylation in myotubes. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 58-65 23835113-6 2013 Ceramide induces beta-cell apoptosis by multiple mechanisms namely; activation of extrinsic apoptotic pathway, increasing cytochrome c release, free radical generation, induction of endoplasmic reticulum stress and inhibition of Akt. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 229-232 23835113-7 2013 Ceramide also modulates many of the insulin signaling intermediates such as insulin receptor substrate, Akt, Glut-4, and it causes insulin resistance. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 104-107 22652149-7 2012 Inhibition of the ceramide-responsive JNK and PP2A pathways did not abolish the effects of ceramide, and JNK phosphorylation was unresponsive to ceramide; however, ceramide significantly inhibited phosphorylation of Akt. Ceramides 18-26 AKT serine/threonine kinase 1 Homo sapiens 216-219 22383528-6 2012 We then showed that in fro/fro fibroblasts, the reduced ceramide was associated with decreased phosphorylation of protein phosphatase 2A (PP2A) and increased phosphorylation of its substrate Akt-p, together with PI3K, PDK1, mTOR (mammalian target of rapamycin), and p70S6K, although PTEN was unaffected. Ceramides 56-64 AKT serine/threonine kinase 1 Homo sapiens 191-194 22583777-6 2012 Increased cellular level of ceramide by the co-administration induced the association between Akt and Protein Phosphatase 1 (PP1) to dephosphorylate Akt, and to introduce a constitutively active Akt (CA-Akt) restored Akt activation and diminished cell growth inhibition. Ceramides 28-36 AKT serine/threonine kinase 1 Homo sapiens 94-97 22583777-6 2012 Increased cellular level of ceramide by the co-administration induced the association between Akt and Protein Phosphatase 1 (PP1) to dephosphorylate Akt, and to introduce a constitutively active Akt (CA-Akt) restored Akt activation and diminished cell growth inhibition. Ceramides 28-36 AKT serine/threonine kinase 1 Homo sapiens 149-152 22583777-6 2012 Increased cellular level of ceramide by the co-administration induced the association between Akt and Protein Phosphatase 1 (PP1) to dephosphorylate Akt, and to introduce a constitutively active Akt (CA-Akt) restored Akt activation and diminished cell growth inhibition. Ceramides 28-36 AKT serine/threonine kinase 1 Homo sapiens 149-152 22583777-6 2012 Increased cellular level of ceramide by the co-administration induced the association between Akt and Protein Phosphatase 1 (PP1) to dephosphorylate Akt, and to introduce a constitutively active Akt (CA-Akt) restored Akt activation and diminished cell growth inhibition. Ceramides 28-36 AKT serine/threonine kinase 1 Homo sapiens 149-152 22583777-6 2012 Increased cellular level of ceramide by the co-administration induced the association between Akt and Protein Phosphatase 1 (PP1) to dephosphorylate Akt, and to introduce a constitutively active Akt (CA-Akt) restored Akt activation and diminished cell growth inhibition. Ceramides 28-36 AKT serine/threonine kinase 1 Homo sapiens 149-152 22764099-2 2012 While investigating the mechanism of Akt inhibition, we found that short-term incubation with these compounds induced rapid shedding of cellular nanovesicles containing EGFR, IGFR and p-Akt that occurred in vitro and in vivo, while prolonged incubation led to cell detachment and death that depended on sphingomyelinase-mediated generation of ceramide. Ceramides 343-351 AKT serine/threonine kinase 1 Homo sapiens 37-40 22764099-4 2012 Similarly, exogenous ceramide also decreased active Akt and induced nanovesicle release. Ceramides 21-29 AKT serine/threonine kinase 1 Homo sapiens 52-55 22764099-7 2012 These results indicate ceramide generation underlies the Akt inhibition and cytotoxicity of this group of agents, and suggests nanovesicle shedding and uptake might potentially propagate their cytotoxicity in vivo. Ceramides 23-31 AKT serine/threonine kinase 1 Homo sapiens 57-60 22320914-6 2012 The PI3K/Akt signaling cascade is altered by direct interaction with ceramides as well as through PTEN upregulation as a downstream target gene of enhanced p53 transcriptional activity. Ceramides 69-78 AKT serine/threonine kinase 1 Homo sapiens 9-12 19959757-1 2010 OBJECTIVE: Ceramide is now recognized as a negative regulator of insulin signaling by impairing protein kinase B (PKB)/Akt activation. Ceramides 11-19 AKT serine/threonine kinase 1 Homo sapiens 114-117 19959757-1 2010 OBJECTIVE: Ceramide is now recognized as a negative regulator of insulin signaling by impairing protein kinase B (PKB)/Akt activation. Ceramides 11-19 AKT serine/threonine kinase 1 Homo sapiens 119-122 19959757-2 2010 In different cells, two distinct mechanisms have been proposed to mediate ceramide inhibition of PKB/Akt: one involving atypical protein kinase C zeta (PKCzeta) and the other the protein phosphatase-2 (PP2A). Ceramides 74-82 AKT serine/threonine kinase 1 Homo sapiens 97-100 19959757-2 2010 In different cells, two distinct mechanisms have been proposed to mediate ceramide inhibition of PKB/Akt: one involving atypical protein kinase C zeta (PKCzeta) and the other the protein phosphatase-2 (PP2A). Ceramides 74-82 AKT serine/threonine kinase 1 Homo sapiens 101-104 19959757-5 2010 RESULTS: Although the PKCzeta-mediated negative effect of ceramide on insulin-stimulated PKB/Akt was dominant in adipocytes, a ceramide action through PP2A outside CEMs, prevented by OKA, was also unraveled. Ceramides 58-66 AKT serine/threonine kinase 1 Homo sapiens 70-96 19959757-8 2010 CONCLUSIONS: Our results show that ceramide can switch from a PKCzeta-dependent mechanism to a PP2A pathway, acting negatively on PKB/Akt, and hence revealing a critical role of CEMs of the PM in this process. Ceramides 35-43 AKT serine/threonine kinase 1 Homo sapiens 130-133 19959757-8 2010 CONCLUSIONS: Our results show that ceramide can switch from a PKCzeta-dependent mechanism to a PP2A pathway, acting negatively on PKB/Akt, and hence revealing a critical role of CEMs of the PM in this process. Ceramides 35-43 AKT serine/threonine kinase 1 Homo sapiens 134-137 19103588-0 2009 Phosphatidylinositol 3-kinase/AKT pathway regulates the endoplasmic reticulum to golgi traffic of ceramide in glioma cells: a link between lipid signaling pathways involved in the control of cell survival. Ceramides 98-106 AKT serine/threonine kinase 1 Homo sapiens 30-33 20954073-4 2010 Treatment of cells with the PP2A inhibitor okadaic acid or with PP2A-Calpha siRNA inhibited ceramide-induced enhanced p27(kip1) protein expression and Akt dephosphorylation, and prevented Skp2 downregulation. Ceramides 92-100 AKT serine/threonine kinase 1 Homo sapiens 151-154 20954073-5 2010 Overexpression of constitutively active Akt attenuated ceramide-induced Skp2 downregulation and p27(kip1) upregulation. Ceramides 55-63 AKT serine/threonine kinase 1 Homo sapiens 40-43 20954073-6 2010 In addition, ceramide stimulated binding of the PP2A catalytic subunit PP2A-Calphabeta to Akt as assessed by immunoprecipitation experiments, indicating that PP2A is involved in the induction of p27(kip1) via inhibition of Akt pathway. Ceramides 13-21 AKT serine/threonine kinase 1 Homo sapiens 90-93 20954073-6 2010 In addition, ceramide stimulated binding of the PP2A catalytic subunit PP2A-Calphabeta to Akt as assessed by immunoprecipitation experiments, indicating that PP2A is involved in the induction of p27(kip1) via inhibition of Akt pathway. Ceramides 13-21 AKT serine/threonine kinase 1 Homo sapiens 223-226 20954073-9 2010 These data reveal that PP2A is a regulator of ceramide-induced p27(kip1) expression via Akt-dependent and Akt-independent pathways. Ceramides 46-54 AKT serine/threonine kinase 1 Homo sapiens 88-91 20954073-9 2010 These data reveal that PP2A is a regulator of ceramide-induced p27(kip1) expression via Akt-dependent and Akt-independent pathways. Ceramides 46-54 AKT serine/threonine kinase 1 Homo sapiens 106-109 19721393-0 2009 Ceramide mediates inhibition of the AKT/eNOS signaling pathway by palmitate in human vascular endothelial cells. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 36-39 19721393-7 2009 Preventing de novo ceramide synthesis abolished the antagonistic effect of palmitate toward the Akt/ eNOS pathway. Ceramides 19-27 AKT serine/threonine kinase 1 Homo sapiens 96-99 19721393-9 2009 CONCLUSIONS: Taken together, we have demonstrated that palmitic acid induces accumulation of ceramide, which appears to mediate palmitic acid"s inhibitory effects on the Akt/eNOS pathway, leading to a significant decrease in NO generation. Ceramides 93-101 AKT serine/threonine kinase 1 Homo sapiens 170-173 19721393-10 2009 Therefore, ceramide is a necessary and sufficient intermediate mediating the inhibition of the AKT/eNOS signaling pathway by palmitate in endothelial cells. Ceramides 11-19 AKT serine/threonine kinase 1 Homo sapiens 95-98 20954073-0 2010 Ceramide produces apoptosis through induction of p27(kip1) by protein phosphatase 2A-dependent Akt dephosphorylation in PC-3 prostate cancer cells. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 95-98 18996148-4 2009 The early inhibition of the neuronal survival pathway regulated by phosphatidil-inositol-3-kinase/protein kinase B or AKT mediated by ceramide may be a relevant early event in the decision of neuronal survival/death. Ceramides 134-142 AKT serine/threonine kinase 1 Homo sapiens 118-121 18996148-8 2009 Subtle and early metabolic alterations caused by inhibition of the PI3K/AKT pathway mediated by ceramide may potentially work with genes associated with neurodegenerative diseases such as Parkinson"s and Alzheimer"s disease. Ceramides 96-104 AKT serine/threonine kinase 1 Homo sapiens 72-75 19103588-3 2009 A reciprocal control between PI3K/Akt and ceramide signaling in glioma cell survival/death is suggested by data demonstrating a protective role of PI3K/Akt on ceramide-induced cell death in glial cells. Ceramides 42-50 AKT serine/threonine kinase 1 Homo sapiens 152-155 19103588-3 2009 A reciprocal control between PI3K/Akt and ceramide signaling in glioma cell survival/death is suggested by data demonstrating a protective role of PI3K/Akt on ceramide-induced cell death in glial cells. Ceramides 159-167 AKT serine/threonine kinase 1 Homo sapiens 34-37 19103588-3 2009 A reciprocal control between PI3K/Akt and ceramide signaling in glioma cell survival/death is suggested by data demonstrating a protective role of PI3K/Akt on ceramide-induced cell death in glial cells. Ceramides 159-167 AKT serine/threonine kinase 1 Homo sapiens 152-155 19103588-4 2009 In this study we investigated the role of the PI3K/Akt pathway in the regulation of the ceramide metabolism in C6 glioma cells, a cell line in which the PI3K/Akt pathway is constitutively activated. Ceramides 88-96 AKT serine/threonine kinase 1 Homo sapiens 51-54 19103588-5 2009 Metabolic experiments performed with different radioactive metabolic precursors of sphingolipids and microscopy studies with fluorescent ceramides demonstrated that the chemical inhibition of PI3K and the transfection with a dominant negative Akt strongly inhibited ceramide utilization for the biosynthesis of complex sphingolipids by controlling the endoplasmic reticulum (ER) to Golgi vesicular transport of ceramide. Ceramides 137-146 AKT serine/threonine kinase 1 Homo sapiens 243-246 19103588-5 2009 Metabolic experiments performed with different radioactive metabolic precursors of sphingolipids and microscopy studies with fluorescent ceramides demonstrated that the chemical inhibition of PI3K and the transfection with a dominant negative Akt strongly inhibited ceramide utilization for the biosynthesis of complex sphingolipids by controlling the endoplasmic reticulum (ER) to Golgi vesicular transport of ceramide. Ceramides 137-145 AKT serine/threonine kinase 1 Homo sapiens 243-246 19103588-5 2009 Metabolic experiments performed with different radioactive metabolic precursors of sphingolipids and microscopy studies with fluorescent ceramides demonstrated that the chemical inhibition of PI3K and the transfection with a dominant negative Akt strongly inhibited ceramide utilization for the biosynthesis of complex sphingolipids by controlling the endoplasmic reticulum (ER) to Golgi vesicular transport of ceramide. Ceramides 266-274 AKT serine/threonine kinase 1 Homo sapiens 243-246 19103588-6 2009 These findings constitute the first evidence for a PI3K/Akt-dependent regulation of vesicle-mediated movements of ceramide in the ER-Golgi district. Ceramides 114-122 AKT serine/threonine kinase 1 Homo sapiens 56-59 19103588-7 2009 Moreover, the findings also suggest the activation of the PI3K/Akt pathway as crucial to coordinate the biosynthesis of membrane complex sphingolipids with cell proliferation and growth and/or to maintain low ceramide levels, especially as concerns those treatments that promote ceramide biosynthesis in the ER. Ceramides 209-217 AKT serine/threonine kinase 1 Homo sapiens 63-66 19103588-7 2009 Moreover, the findings also suggest the activation of the PI3K/Akt pathway as crucial to coordinate the biosynthesis of membrane complex sphingolipids with cell proliferation and growth and/or to maintain low ceramide levels, especially as concerns those treatments that promote ceramide biosynthesis in the ER. Ceramides 279-287 AKT serine/threonine kinase 1 Homo sapiens 63-66 18054155-0 2008 Ceramide induces p38 MAPK-dependent apoptosis and Bax translocation via inhibition of Akt in HL-60 cells. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 86-89 18397976-12 2008 Whether the decrease in AKT can be attributed to the trend to higher muscle ceramide remains unanswered. Ceramides 76-84 AKT serine/threonine kinase 1 Homo sapiens 24-27 18054155-5 2008 Overexpression of Akt also led to suppression of Bax translocation to mitochondria during ceramide-induced apoptosis in HL-60 cells. Ceramides 90-98 AKT serine/threonine kinase 1 Homo sapiens 18-21 17666435-8 2007 These results indicate a role for GSK-3beta in ceramide-induced apoptosis, in which GSK-3beta acts downstream of PP2A and the PI 3-kinase-Akt pathway, and upstream of caspase-2 and caspase-8. Ceramides 47-55 AKT serine/threonine kinase 1 Homo sapiens 138-141 17471535-0 2007 PKB/Akt inhibits ceramide-induced apoptosis in neuroblastoma cells by blocking apoptosis-inducing factor (AIF) translocation. Ceramides 17-25 AKT serine/threonine kinase 1 Homo sapiens 0-3 17471535-0 2007 PKB/Akt inhibits ceramide-induced apoptosis in neuroblastoma cells by blocking apoptosis-inducing factor (AIF) translocation. Ceramides 17-25 AKT serine/threonine kinase 1 Homo sapiens 4-7 17471535-4 2007 Treatment of the human neuroblastoma cell line SH-SY5Y with ceramide induced dephosphorylation of the PKB/Akt kinase and subsequent mitochondrial dysfunction. Ceramides 60-68 AKT serine/threonine kinase 1 Homo sapiens 102-105 17471535-4 2007 Treatment of the human neuroblastoma cell line SH-SY5Y with ceramide induced dephosphorylation of the PKB/Akt kinase and subsequent mitochondrial dysfunction. Ceramides 60-68 AKT serine/threonine kinase 1 Homo sapiens 106-109 17471535-7 2007 Furthermore, overexpression of active PKB/Akt or Bcl-2 successfully blocked ceramide-induced neuronal cell death through inhibition of the translocation of apoptosis-inducing factor. Ceramides 76-84 AKT serine/threonine kinase 1 Homo sapiens 38-41 17471535-7 2007 Furthermore, overexpression of active PKB/Akt or Bcl-2 successfully blocked ceramide-induced neuronal cell death through inhibition of the translocation of apoptosis-inducing factor. Ceramides 76-84 AKT serine/threonine kinase 1 Homo sapiens 42-45 17259384-0 2007 Ceramide- and oxidant-induced insulin resistance involve loss of insulin-dependent Rac-activation and actin remodeling in muscle cells. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 83-86 17259384-8 2007 We propose that ceramide and oxidative stress can each affect two independent arms of insulin signaling to GLUT4 at distinct steps, Rac-GTP loading and Akt phosphorylation. Ceramides 16-24 AKT serine/threonine kinase 1 Homo sapiens 132-135 15361788-4 2004 Some of the proapoptotic actions of ceramide are to facilitate assembly of death receptor complexes in the plasma membrane, to prevent the activation of protein kinase B/Akt, and to promote the activation of caspase 3. Ceramides 36-44 AKT serine/threonine kinase 1 Homo sapiens 170-173 17259384-8 2007 We propose that ceramide and oxidative stress can each affect two independent arms of insulin signaling to GLUT4 at distinct steps, Rac-GTP loading and Akt phosphorylation. Ceramides 16-24 AKT serine/threonine kinase 1 Homo sapiens 152-155 17225464-5 2006 In addition, the ceramide analog decreased the phospho-Akt and phospho-Bad levels. Ceramides 17-25 AKT serine/threonine kinase 1 Homo sapiens 55-58 15968641-2 2005 Here we present evidence that ceramide recruits the tumor suppressor PTEN (phosphatase and tensin homolog deleted from chromosome 10) into membrane microdomains (rafts), where it could act to reduce the levels of polyphosphoinositides necessary for the activation of Akt. Ceramides 30-38 AKT serine/threonine kinase 1 Homo sapiens 267-270 16290312-0 2005 IGF-I protects cortical neurons against ceramide-induced apoptosis via activation of the PI-3K/Akt and ERK pathways; is this protection independent of CREB and Bcl-2? Ceramides 40-48 AKT serine/threonine kinase 1 Homo sapiens 95-98 16290312-5 2005 Ceramide decreases Akt phosphorylation during apoptotic process whereas a simultaneous treatment with IGF-I increases Akt phosphorylation. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 19-22 15978590-5 2005 Based on these and our previous observations, we propose a working model for C1P in which inhibition of acid sphingomyelinase and the subsequent decrease in ceramide levels would allow cell signaling through stimulation of the PI3-K/PKB pathway to promote cell survival. Ceramides 157-165 AKT serine/threonine kinase 1 Homo sapiens 233-236 15781658-6 2005 Enforced expression of constitutively active mitogen-activated protein kinase kinase (MEK) 1 or myristoylated Akt blocked HDACI/perifosine-mediated ceramide production and cell death, suggesting that MEK/ERK and Akt inactivation play a primary role in these phenomena. Ceramides 148-156 AKT serine/threonine kinase 1 Homo sapiens 110-113 15781658-6 2005 Enforced expression of constitutively active mitogen-activated protein kinase kinase (MEK) 1 or myristoylated Akt blocked HDACI/perifosine-mediated ceramide production and cell death, suggesting that MEK/ERK and Akt inactivation play a primary role in these phenomena. Ceramides 148-156 AKT serine/threonine kinase 1 Homo sapiens 212-215 15220355-0 2004 Regulation of insulin action by ceramide: dual mechanisms linking ceramide accumulation to the inhibition of Akt/protein kinase B. Ceramides 32-40 AKT serine/threonine kinase 1 Homo sapiens 109-112 15220355-0 2004 Regulation of insulin action by ceramide: dual mechanisms linking ceramide accumulation to the inhibition of Akt/protein kinase B. Ceramides 66-74 AKT serine/threonine kinase 1 Homo sapiens 109-112 15220355-4 2004 Surprisingly the findings obtained with these separate domains reveal that ceramide blocks insulin stimulation of Akt/PKB by two independent mechanisms. Ceramides 75-83 AKT serine/threonine kinase 1 Homo sapiens 118-121 15220355-3 2004 Herein we utilized deletion constructs encoding two different functional domains of Akt/PKB to identify which region of the enzyme conferred responsiveness to ceramide. Ceramides 159-167 AKT serine/threonine kinase 1 Homo sapiens 84-91 15220355-5 2004 First, using the isolated pleckstrin homology domain, we found that ceramide specifically blocks the translocation of Akt/PKB, but not its upstream activator phosphoinositide-dependent kinase-1, to the plasma membrane. Ceramides 68-76 AKT serine/threonine kinase 1 Homo sapiens 118-125 15220355-6 2004 Second, using a construct lacking this pleckstrin homology domain, which does not require translocation for activation, we found that ceramide stimulates the dephosphorylation of Akt/PKB by protein phosphatase 2A. Ceramides 134-142 AKT serine/threonine kinase 1 Homo sapiens 179-182 15220355-4 2004 Surprisingly the findings obtained with these separate domains reveal that ceramide blocks insulin stimulation of Akt/PKB by two independent mechanisms. Ceramides 75-83 AKT serine/threonine kinase 1 Homo sapiens 114-117 15220355-6 2004 Second, using a construct lacking this pleckstrin homology domain, which does not require translocation for activation, we found that ceramide stimulates the dephosphorylation of Akt/PKB by protein phosphatase 2A. Ceramides 134-142 AKT serine/threonine kinase 1 Homo sapiens 183-186 12676512-5 2003 It is through these protein phosphatases that ceramide can indirectly inhibit kinases that are key components of pro-growth signaling processes such as the classical and novel PKC isoforms and protein kinase B (PKB; also known as Akt). Ceramides 46-54 AKT serine/threonine kinase 1 Homo sapiens 211-214 15220355-8 2004 Moreover the results obtained provide a unifying theory to account for the numerous dissenting reports investigating the actions of ceramide toward Akt/PKB. Ceramides 132-140 AKT serine/threonine kinase 1 Homo sapiens 148-155 14693694-11 2004 This twofold increase in ceramide may be involved in the decrease in Akt phosphorylation observed after insulin infusion and could theoretically play a role in the reduced ability of insulin to stimulate glucose uptake in skeletal muscle from obese subjects. Ceramides 25-33 AKT serine/threonine kinase 1 Homo sapiens 69-72 14560023-0 2003 Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 105-108 12975495-8 2003 Liposomal ceramide formulations inhibited phosphorylated Akt levels and stimulated caspase-3/7 activity more effectively than nonliposomal ceramide, events consistent with apoptosis. Ceramides 10-18 AKT serine/threonine kinase 1 Homo sapiens 57-60 12676512-5 2003 It is through these protein phosphatases that ceramide can indirectly inhibit kinases that are key components of pro-growth signaling processes such as the classical and novel PKC isoforms and protein kinase B (PKB; also known as Akt). Ceramides 46-54 AKT serine/threonine kinase 1 Homo sapiens 230-233 12482824-7 2002 CONCLUSIONS: SMC proliferation elicited by moderate concentrations of oxLDL involves the sphingomyelin/ceramide/sphingosine-1-phosphate pathway, which leads to extracellular regulated kinase 1/2 activation and DNA synthesis, and the EGFR/PI-3K/Akt pathway, which prevents the apoptotic effect of oxLDL. Ceramides 103-111 AKT serine/threonine kinase 1 Homo sapiens 244-247 12706871-5 2003 Exposure of CNE2 cells to ceramide resulted in a dose-dependent up-regulation of the cyclin-dependent kinase inhibitor p27 and a decrease of phospho-Akt without reduced expression of total AKT protein. Ceramides 26-34 AKT serine/threonine kinase 1 Homo sapiens 149-152 12706871-5 2003 Exposure of CNE2 cells to ceramide resulted in a dose-dependent up-regulation of the cyclin-dependent kinase inhibitor p27 and a decrease of phospho-Akt without reduced expression of total AKT protein. Ceramides 26-34 AKT serine/threonine kinase 1 Homo sapiens 189-192 12706871-8 2003 In addition, up-regulation of p27 resulting from ceramide treatment may be due to the interruption of Akt, but decrease of phospho-Akt is independent of PI3K function or PTEN protein expression. Ceramides 49-57 AKT serine/threonine kinase 1 Homo sapiens 102-105 12525490-4 2003 Preventing de novo ceramide synthesis negated the antagonistic effect of saturated FFAs toward Akt/protein kinase B. Ceramides 19-27 AKT serine/threonine kinase 1 Homo sapiens 95-98 12691738-0 2003 Ceramide-induced neuronal apoptosis is associated with dephosphorylation of Akt, BAD, FKHR, GSK-3beta, and induction of the mitochondrial-dependent intrinsic caspase pathway. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 76-79 12691738-4 2003 We show that ceramide treatment initiates a cascade of biochemical alterations associated with cell death: earliest signal transduction changes involve Akt dephosphorylation and inactivation followed by dephosphorylation of proapoptotic regulators such as BAD (proapoptotic Bcl-2 family member), Forkhead family transcription factors, glycogen synthase kinase 3-beta, mitochondrial depolarization and permeabilization, release of cytochrome c into the cytosol, and caspase-3 activation. Ceramides 13-21 AKT serine/threonine kinase 1 Homo sapiens 152-155 12213802-0 2002 Ceramide and reactive oxygen species generated by H2O2 induce caspase-3-independent degradation of Akt/protein kinase B. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 99-102 11821946-0 2002 Low glucose-enhanced TRAIL cytotoxicity is mediated through the ceramide-Akt-FLIP pathway. Ceramides 64-72 AKT serine/threonine kinase 1 Homo sapiens 73-76 11706021-0 2002 Akt protein kinase inhibits non-apoptotic programmed cell death induced by ceramide. Ceramides 75-83 AKT serine/threonine kinase 1 Homo sapiens 0-3 11706021-6 2002 However, strikingly, the ceramide-induced non-apoptotic cell death was inhibited by the activation of the Akt/protein kinase B pathway through the expression of a constitutively active version of Akt. Ceramides 25-33 AKT serine/threonine kinase 1 Homo sapiens 106-109 11706021-6 2002 However, strikingly, the ceramide-induced non-apoptotic cell death was inhibited by the activation of the Akt/protein kinase B pathway through the expression of a constitutively active version of Akt. Ceramides 25-33 AKT serine/threonine kinase 1 Homo sapiens 196-199 12213802-2 2002 H2O2 produced early activation of Akt/PKB and also DNA damage that was followed by stabilization of p53 levels, formation of reactive oxygen species (ROS), and generation of ceramide through activation of a glutathione-sensitive neutral sphingomyelinase. Ceramides 174-182 AKT serine/threonine kinase 1 Homo sapiens 34-41 12213802-5 2002 Proteolysis of Akt was prevented by exogenous addition of glutathione, indicating a role of ROS and ceramide in Akt degradation. Ceramides 100-108 AKT serine/threonine kinase 1 Homo sapiens 15-18 12213802-10 2002 Our results suggest that strong oxidants generate intracellular ROS and ceramide which in term lead to down-regulation of Akt by dephosphorylation and caspase-3-independent proteolysis. Ceramides 72-80 AKT serine/threonine kinase 1 Homo sapiens 122-125 11821946-7 2002 The elevation of ceramide may cause dephosphorylation of Akt and maintain dephosphorylation of Akt in the presence of TRAIL and then subsequently down-regulate the expression of FLIP. Ceramides 17-25 AKT serine/threonine kinase 1 Homo sapiens 57-60 11821946-7 2002 The elevation of ceramide may cause dephosphorylation of Akt and maintain dephosphorylation of Akt in the presence of TRAIL and then subsequently down-regulate the expression of FLIP. Ceramides 17-25 AKT serine/threonine kinase 1 Homo sapiens 95-98 11821946-8 2002 Taken together, the present studies suggest that glucose deprivation enhances TRAIL-induced cytotoxicity through the ceramide-Akt-FLIP pathway. Ceramides 117-125 AKT serine/threonine kinase 1 Homo sapiens 126-129 11751589-3 2002 In this study we assessed whether ceramide and/or glucosamine, two known insulin-signaling antagonists, also affected the PI3K/Akt-independent signal. Ceramides 34-42 AKT serine/threonine kinase 1 Homo sapiens 127-130 11171115-4 2001 Here we show that ceramide analogues specifically prevent the recruitment of the PtdIns(3,4,5)P(3)-binding proteins Akt/protein kinase B (PKB) or the general receptor for phosphoinositides-1 (GRP1). Ceramides 18-26 AKT serine/threonine kinase 1 Homo sapiens 116-119 11679435-8 2001 Our results indicate that ceramide produced by TNF-alpha induces insulin resistance in brown adipocytes by maintaining Akt in an inactive dephosphorylated state. Ceramides 26-34 AKT serine/threonine kinase 1 Homo sapiens 119-122 11527425-0 2001 Ceramide blocks PDGF-induced DNA synthesis in mesangial cells via inhibition of Akt kinase in the absence of apoptosis. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 80-83 11527425-11 2001 Incubation of mesangial cells with C2 ceramide inhibited PDGF-induced Akt activity. Ceramides 38-46 AKT serine/threonine kinase 1 Homo sapiens 70-73 11527425-14 2001 These data provide the first evidence that in mesangial cells, ceramide cross-talks with PI 3 kinase-dependent Akt kinase to inhibit PDGF-induced DNA synthesis without inducing apoptosis. Ceramides 63-71 AKT serine/threonine kinase 1 Homo sapiens 111-114 11751455-8 2001 Finally, we demonstrate that in cells treated with ceramide, cleavage of TIAM1 coincided with the inactivation of endogenous Rac. Ceramides 51-59 AKT serine/threonine kinase 1 Homo sapiens 125-128 11698477-0 2001 Ceramide regulates lipopolysaccharide-induced phosphatidylinositol 3-kinase and Akt activity in human alveolar macrophages. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 80-83 11698477-5 2001 We found that ceramide exposure activated PI 3-kinase and Akt. Ceramides 14-22 AKT serine/threonine kinase 1 Homo sapiens 58-61 11698477-6 2001 When we blocked LPS-induced ceramide with the inhibitor D609, we blocked LPS-induced PI 3-kinase and Akt activation. Ceramides 28-36 AKT serine/threonine kinase 1 Homo sapiens 101-104 11679435-0 2001 Ceramide mediates insulin resistance by tumor necrosis factor-alpha in brown adipocytes by maintaining Akt in an inactive dephosphorylated state. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 103-106 11480555-13 2001 Ceramide activation of protein phosphatases has been shown to promote inactivation of a number of pro-growth cellular regulators including the kinases PKC alpha and Akt, Bcl2 and the retinoblastoma protein. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 165-168 11312266-2 2001 The precise mechanism that p75NTR uses to promote cell death is not certain, but one possibility is that p75NTR-dependent ceramide accumulation inhibits phosphatidylinositol 3-kinase-mediated Akt activation. Ceramides 122-130 AKT serine/threonine kinase 1 Homo sapiens 192-195 11171115-4 2001 Here we show that ceramide analogues specifically prevent the recruitment of the PtdIns(3,4,5)P(3)-binding proteins Akt/protein kinase B (PKB) or the general receptor for phosphoinositides-1 (GRP1). Ceramides 18-26 AKT serine/threonine kinase 1 Homo sapiens 138-141 11171115-5 2001 Specifically, the short-chain ceramide derivative C2-ceramide inhibited the platelet-derived growth factor (PDGF)-stimulated translocation of full-length Akt/PKB, as well as truncated proteins encoding only the PH domains of Akt/PKB or GRP1. Ceramides 30-38 AKT serine/threonine kinase 1 Homo sapiens 154-161 11171115-5 2001 Specifically, the short-chain ceramide derivative C2-ceramide inhibited the platelet-derived growth factor (PDGF)-stimulated translocation of full-length Akt/PKB, as well as truncated proteins encoding only the PH domains of Akt/PKB or GRP1. Ceramides 30-38 AKT serine/threonine kinase 1 Homo sapiens 225-232 10669728-1 2000 Previous studies have indicated that proapoptotic stresses downregulate the phosphatidylinositol 3-kinase [PI(3)K]/Akt survival pathway via the activation of acid-sphingomyelinase (A-SMase) and ceramide production. Ceramides 194-202 AKT serine/threonine kinase 1 Homo sapiens 115-118 11162641-0 2001 Ceramide induces the dephosphorylation and inhibition of constitutively activated Akt in PTEN negative U87mg cells. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 82-85 10788440-0 2000 Ceramide inhibits protein kinase B/Akt by promoting dephosphorylation of serine 473. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 35-38 10694479-9 2000 Our data demonstrated that inhibition of anti-apoptotic kinases, such as Akt and ERK1/2, may play an important role in ceramide-mediated apoptosis of rheumatoid synovial cells. Ceramides 119-127 AKT serine/threonine kinase 1 Homo sapiens 73-76 11042022-0 2000 Akt mediates insulin rescue from apoptosis in brown adipocytes: effect of ceramide. Ceramides 74-82 AKT serine/threonine kinase 1 Homo sapiens 0-3 11042022-7 2000 Ceramide treatment blunted Akt activity but not PI 3-kinase activity, and insulin and EGF were unable to activate Akt. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 27-30 11042022-8 2000 Ceramide also caused apoptosis in cells transfected with a constitutively active Akt construct, since phosphorylation of Akt was impaired under these experimental conditions. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 81-84 11042022-8 2000 Ceramide also caused apoptosis in cells transfected with a constitutively active Akt construct, since phosphorylation of Akt was impaired under these experimental conditions. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 121-124 10585289-5 1999 Ceramide, an intermediate of several apoptotic pathways, interferes with growth factor-mediated Akt activation. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 96-99 10585289-6 1999 Ceramide induced endothelial cell death and abolished angiopoietin-1-mediated activation of Akt and the effect on cell survival. Ceramides 0-8 AKT serine/threonine kinase 1 Homo sapiens 92-95 10842662-7 1999 Interestingly, soluble analogues of ceramide antagonize both insulin"s activation of Akt/PKB as well as its stimulation of glucose transport, consistent with a causal relationship between the two. Ceramides 36-44 AKT serine/threonine kinase 1 Homo sapiens 89-92