PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7929167-0 1994 Forskolin specifically inhibits the bacterial galactose-H+ transport protein, GalP. Colforsin 0-9 galanin like peptide Homo sapiens 78-82 7929167-2 1994 Here we show that forskolin is a remarkably specific inhibitor of energized D-galactose transport by the GalP sugar-H+ symport protein of Escherichia coli. Colforsin 18-27 galanin like peptide Homo sapiens 105-109 7929167-4 1994 However, unlike GLUT1, photoactivation of the [3H]forskolin-GalP complex fails to incorporate radioactivity covalently into the protein, in contrast to the effective incorporation of radioactivity from [3H]cytochalasin B into both proteins. Colforsin 50-59 galanin like peptide Homo sapiens 60-64 7929167-5 1994 However, 3-[125I]iodo-4-azidophenethylamido-7-O-succinyldesacetylforskol in ([125I]APS-forskolin), which labels GLUT1, is a potent labeling reagent for GalP and, to a lesser extent, for AraE. Colforsin 87-96 galanin like peptide Homo sapiens 152-156 7929167-7 1994 Equilibrium binding studies using membranes from an E. coli strain that overexpresses GalP reveal a single set of high affinity binding sites for [3H]forskolin with a Kd of 1.3-1.4 microM, probably forming a 1:1 complex, compared with a value of 7.5 microM for GLUT1. Colforsin 150-159 galanin like peptide Homo sapiens 86-90 7929167-8 1994 Sugar substrates of GalP and cytochalasin B displace forskolin from the protein. Colforsin 53-62 galanin like peptide Homo sapiens 20-24 7929167-10 1994 Forskolin and [125I]APS-forskolin, therefore, constitute novel probes for exploring the structure-activity relationship of the bacterial GalP protein. Colforsin 0-9 galanin like peptide Homo sapiens 137-141 7929167-10 1994 Forskolin and [125I]APS-forskolin, therefore, constitute novel probes for exploring the structure-activity relationship of the bacterial GalP protein. Colforsin 24-33 galanin like peptide Homo sapiens 137-141 24804563-5 2014 The GalP/GLUT inhibitor forskolin was added to the ILV-labelled sample inducing a pronounced chemical shift change in one Ile residue and more subtle changes in other methyl groups. Colforsin 24-33 galanin like peptide Homo sapiens 4-8