PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18296025-5 2008 This redistribution of BRCA1 is partially sensitive to leptomycin B, indicating that CRM-1-mediated nuclear export is involved. leptomycin B 55-67 exportin 1 Homo sapiens 85-90 17440726-6 2008 Leptomycin B, a potent inhibitor of Crm1-mediated nuclear export, is also a very potent G(2) checkpoint inhibitor. leptomycin B 0-12 exportin 1 Homo sapiens 36-40 18287525-6 2008 In cells with unperturbed centrosome nucleation, defective RanGAP1 recruitment at kinetochores after treatment with the Crm1 inhibitor leptomycin B activates kinetochore microtubule nucleation after cold. leptomycin B 135-147 exportin 1 Homo sapiens 120-124 18316374-9 2008 TRIP-Br2 was shown to be stabilized in G(2)/M phase cells through nuclear entrapment, either by deletion of the acidic C-terminal TAD, which includes the NES motif, or by leptomycin B-mediated inhibition of the CRM1-dependent nuclear export machinery. leptomycin B 171-183 exportin 1 Homo sapiens 211-215 18306389-8 2008 Because it was demonstrated previously that blocking of CRM1 by leptomycin B (LMB) contributes to the inhibition of nuclear export, the authors used a set of mechanistic assays to study the effects of CRM1 inhibition in cancer cells. leptomycin B 64-76 exportin 1 Homo sapiens 56-60 18306389-8 2008 Because it was demonstrated previously that blocking of CRM1 by leptomycin B (LMB) contributes to the inhibition of nuclear export, the authors used a set of mechanistic assays to study the effects of CRM1 inhibition in cancer cells. leptomycin B 78-81 exportin 1 Homo sapiens 56-60 17715235-9 2007 However, we found that the cytoplasmic accumulation of pp65 late in infection was sensitive to the Crm1 inhibitor leptomycin B. leptomycin B 114-126 exportin 1 Homo sapiens 99-103 17693924-3 2008 Leptomycin B specifically inhibits export of IkappaBalpha and the inactive IkappaBalpha/Rel A complex via the nuclear export protein exportin 1. leptomycin B 0-12 exportin 1 Homo sapiens 133-143 19066035-2 2008 Analysis of the nucleocytoplasmic protein shuttling has been greatly facilitated by the use of leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export. leptomycin B 95-107 exportin 1 Homo sapiens 131-135 19066035-2 2008 Analysis of the nucleocytoplasmic protein shuttling has been greatly facilitated by the use of leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export. leptomycin B 109-112 exportin 1 Homo sapiens 131-135 17652399-8 2007 Leptomycin B treatment resulted in nuclear accumulation of nsP2, demonstrating that nuclear export of nsP2 is mediated via the CRM1 nuclear export pathway. leptomycin B 0-12 exportin 1 Homo sapiens 127-131 17430897-5 2007 Incubation with leptomycin B prior to hypoxia abolished nuclear HO-1 and the faster migrating band on Western analysis, suggesting that this process was facilitated by CRM1. leptomycin B 16-28 exportin 1 Homo sapiens 168-172 17585339-4 2007 Here, we demonstrate that etoposide and ionizing radiation induce the exit of BID from the nucleus and that leptomycin B, a specific inhibitor of the nuclear export receptor CRM1, prevents the nuclear exit of BID. leptomycin B 108-120 exportin 1 Homo sapiens 174-178 17693610-1 2007 We used the Crm1 inhibitor leptomycin B (LMB) to examine a possible involvement of nuclear export in estrogen receptor alpha (ER) level and function in MCF-7 breast carcinoma cells. leptomycin B 27-39 exportin 1 Homo sapiens 12-16 17616529-8 2007 Formation of the FGF-1-exportin-1-Ran-GTP complex in vitro as well as nuclear export of FGF-1 in vivo was dependent on phosphorylation of FGF-1, and it was abolished by leptomycin B. leptomycin B 169-181 exportin 1 Homo sapiens 23-33 17570491-3 2007 Export of EcR and EcR/Usp is mediated by exportin-1 (CRM-1) as shown by the inhibiting effect of leptomycin B (LMB). leptomycin B 97-109 exportin 1 Homo sapiens 41-51 17570491-3 2007 Export of EcR and EcR/Usp is mediated by exportin-1 (CRM-1) as shown by the inhibiting effect of leptomycin B (LMB). leptomycin B 97-109 exportin 1 Homo sapiens 53-58 17570491-3 2007 Export of EcR and EcR/Usp is mediated by exportin-1 (CRM-1) as shown by the inhibiting effect of leptomycin B (LMB). leptomycin B 111-114 exportin 1 Homo sapiens 41-51 17570491-3 2007 Export of EcR and EcR/Usp is mediated by exportin-1 (CRM-1) as shown by the inhibiting effect of leptomycin B (LMB). leptomycin B 111-114 exportin 1 Homo sapiens 53-58 17074802-7 2007 Inhibition of the nuclear exporter CRM1 by leptomycin B did not interfere with NEMO nuclear export. leptomycin B 43-55 exportin 1 Homo sapiens 35-39 17412691-5 2007 Treatment with the nuclear export inhibitor leptomycin B and mutagenesis analysis using green fluorescent protein-fused Id1 revealed CRM1/exportin-dependent nuclear export of Id1 in HUVECs on Matrigel. leptomycin B 44-56 exportin 1 Homo sapiens 133-137 17405816-7 2007 Inhibition of the nuclear export factor, CRM1, using leptomycin B results in a change in the dynamics of interaction of newly imported snRNPs with CBs. leptomycin B 53-65 exportin 1 Homo sapiens 41-45 16964287-7 2007 These results indicated that LMB inhibited the chromosomal region maintenance 1 (CRM1)-dependent nuclear export of PP2A, resulting in sustained dephosphorylation in the nucleus. leptomycin B 29-32 exportin 1 Homo sapiens 47-79 16964287-7 2007 These results indicated that LMB inhibited the chromosomal region maintenance 1 (CRM1)-dependent nuclear export of PP2A, resulting in sustained dephosphorylation in the nucleus. leptomycin B 29-32 exportin 1 Homo sapiens 81-85 17182034-7 2007 The CRM1 inhibitor Leptomycin B increases the nuclear localization of GFP-tagged topo IIalpha with a mutant NLS, suggesting that its export is being inhibited. leptomycin B 19-31 exportin 1 Homo sapiens 4-8 17245118-6 2007 In addition, the sensitivity of CYFIP2 protein subcellular localization to Leptomycin-B, a CRM-1/Exportin inhibitor, suggests that the biological functions of CYFIP2 may extend from the cytoplasmic compartment into the nucleus of the cell. leptomycin B 75-87 exportin 1 Homo sapiens 91-96 17142760-5 2006 Treatment of cells with leptomycin B, a specific inhibitor of CRM-1, completely inhibited IFN-gamma induction of HLA-A. leptomycin B 24-36 exportin 1 Homo sapiens 62-67 17064665-3 2006 Here, we report that leptomycin B (LMB), a specific inhibitor of CRM1-dependent nuclear protein export, inhibits TNFalpha release from LPS-stimulated human peripheral blood neutrophils and mononuclear cells. leptomycin B 21-33 exportin 1 Homo sapiens 65-69 17064665-3 2006 Here, we report that leptomycin B (LMB), a specific inhibitor of CRM1-dependent nuclear protein export, inhibits TNFalpha release from LPS-stimulated human peripheral blood neutrophils and mononuclear cells. leptomycin B 35-38 exportin 1 Homo sapiens 65-69 17099693-3 2006 We show that inhibition of the Survivin-Crm1 interaction by treatment with leptomycin B or by RNA-interference-mediated Crm1 depletion prevents centromeric targeting of Survivin. leptomycin B 75-87 exportin 1 Homo sapiens 40-44 16954218-10 2006 Treatment of the cells with leptomycin B, an inhibitor of the major exportin CRM1, reversed this exclusion and led to nuclear accumulation and partial recovery of the activity of the HIF-1alpha mutants. leptomycin B 28-40 exportin 1 Homo sapiens 77-81 17065226-4 2006 We showed that CRM1 and E1A coimmunoprecipitated and that blockage of CRM1 function by leptomycin B or small interfering RNA resulted in the nuclear localization of E1A. leptomycin B 87-99 exportin 1 Homo sapiens 70-74 16720834-7 2006 The specific Crm1/exportin-1 inhibitor leptomycin-B relocated NPM mutants from cytoplasm to nucleus of primary NPMc(+) AML cells, demonstrating that nuclear export is NES dependent. leptomycin B 39-49 exportin 1 Homo sapiens 13-17 16882664-7 2006 When treated with leptomycin B, Jab1-facilitated nuclear exclusion of WNVCp was prevented, which indicated that the CRM1 complex is required for Jab1-facilitated nuclear export of WNVCp. leptomycin B 18-30 exportin 1 Homo sapiens 116-120 16968744-6 2006 The localization of Kank in the cells before and after treatment with leptomycin B suggested that the transportation of Kank from the nucleus to the cytoplasm was mediated by a CRM1-dependent mechanism. leptomycin B 70-82 exportin 1 Homo sapiens 177-181 17005680-7 2006 We show that nuclear export dictated by this bUL47 NES is sensitive to leptomycin B (LMB) and therefore dependent on the export receptor CRM-1. leptomycin B 71-83 exportin 1 Homo sapiens 137-142 17005680-7 2006 We show that nuclear export dictated by this bUL47 NES is sensitive to leptomycin B (LMB) and therefore dependent on the export receptor CRM-1. leptomycin B 85-88 exportin 1 Homo sapiens 137-142 16720834-7 2006 The specific Crm1/exportin-1 inhibitor leptomycin-B relocated NPM mutants from cytoplasm to nucleus of primary NPMc(+) AML cells, demonstrating that nuclear export is NES dependent. leptomycin B 39-49 exportin 1 Homo sapiens 18-28 16777165-5 2006 This Rev-dependent translocation was specifically inhibited by leptomycin B suggesting export via the CRM1-dependent pathway used by late lentiviral transcripts. leptomycin B 63-75 exportin 1 Homo sapiens 102-106 16484227-7 2006 Likewise, inhibition of the CRM1 nuclear export pathway by leptomycin B or overexpression of a defective form of the nucleoporin Nup214/CAN diminished cytoplasmic CD83 mRNA levels. leptomycin B 59-71 exportin 1 Homo sapiens 28-32 16705178-3 2006 Treatment of cells with leptomycin B (LMB), a specific inhibitor of the nuclear export signal (NES)-dependent receptor CRM1, causes nuclear accumulation of Dok1. leptomycin B 24-36 exportin 1 Homo sapiens 119-123 16705178-3 2006 Treatment of cells with leptomycin B (LMB), a specific inhibitor of the nuclear export signal (NES)-dependent receptor CRM1, causes nuclear accumulation of Dok1. leptomycin B 38-41 exportin 1 Homo sapiens 119-123 16552725-9 2006 Finally, we found that nuclear export of EGFR may involve CRM1 exportin as we detected EGFR/CRM1 interaction and markedly increased nuclear EGFR following exposure to leptomycin B, a CRM1 inhibitor. leptomycin B 167-179 exportin 1 Homo sapiens 58-62 16552725-9 2006 Finally, we found that nuclear export of EGFR may involve CRM1 exportin as we detected EGFR/CRM1 interaction and markedly increased nuclear EGFR following exposure to leptomycin B, a CRM1 inhibitor. leptomycin B 167-179 exportin 1 Homo sapiens 92-96 16552725-9 2006 Finally, we found that nuclear export of EGFR may involve CRM1 exportin as we detected EGFR/CRM1 interaction and markedly increased nuclear EGFR following exposure to leptomycin B, a CRM1 inhibitor. leptomycin B 167-179 exportin 1 Homo sapiens 92-96 16507996-4 2006 Leptomycin B, chromosome region maintenance 1 (CRM1) overexpression, and heterokaryon experiments indicate that Bright actively shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. leptomycin B 0-12 exportin 1 Homo sapiens 180-184 16306087-8 2006 Leptomycin B, a specific inhibitor of CRM1-dependent nuclear export of pp32, significantly reduced ACTH-induced VEGF mRNA levels. leptomycin B 0-12 exportin 1 Homo sapiens 38-42 16378739-2 2006 In this report, we describe the effects of exogenous LEF-1 and of treatment with leptomycin B (LMB), a specific inhibitor of CRM1-medicated nuclear export, on the nuclear localization and export of beta-catenin. leptomycin B 81-93 exportin 1 Homo sapiens 125-129 16378739-2 2006 In this report, we describe the effects of exogenous LEF-1 and of treatment with leptomycin B (LMB), a specific inhibitor of CRM1-medicated nuclear export, on the nuclear localization and export of beta-catenin. leptomycin B 95-98 exportin 1 Homo sapiens 125-129 16388893-3 2006 The discovery of most commonly utilized CRM1/exportin1 dependent nuclear export pathway is attributed to a potent nuclear export inhibitor leptomycin B that helped dissecting this and other nuclear export pathways. leptomycin B 139-151 exportin 1 Homo sapiens 40-44 16388893-3 2006 The discovery of most commonly utilized CRM1/exportin1 dependent nuclear export pathway is attributed to a potent nuclear export inhibitor leptomycin B that helped dissecting this and other nuclear export pathways. leptomycin B 139-151 exportin 1 Homo sapiens 45-54 16110492-5 2006 The taxol-dependent cytoplasmic accumulation of RUNX2 is inhibited by leptomycin B, which blocks CRM-1 dependent nuclear export, and is not affected by the protein synthesis inhibitor cycloheximide. leptomycin B 70-82 exportin 1 Homo sapiens 97-102 16319068-8 2006 Leptomycin B could inhibit the nuclear export of prohibitin showing that it was a CRM-1-dependent event driven by Ran GTPase. leptomycin B 0-12 exportin 1 Homo sapiens 82-87 16140268-6 2005 Moreover, nuclear retention of STAT3 RA by a chromosomal region maintenance 1 (CRM1) inhibitor, leptomycin B, decreased the enhanced STAT3 activation by stimuli. leptomycin B 96-108 exportin 1 Homo sapiens 45-77 16414989-8 2006 Mutational and functional analysis revealed that the methionine residues within the MetR domain are critical for the activity of the NES of P. Furthermore, leptomycin B or small interfering RNA for inhibition of CRM1 strongly suggested that a CRM1-dependent pathway mediates nuclear export of P. Fluorescence loss in photobleaching analysis confirmed the nucleocytoplasmic shuttling of P. Moreover, we revealed that the nuclear export of P is not involved in the inhibition of the polymerase activity by X in the BDV minireplicon system. leptomycin B 156-168 exportin 1 Homo sapiens 243-247 16302269-4 2006 Western blot analysis of nuclear and cytoplasmic fractions identified Bok in both the nucleus and the cytoplasm of HEK 293T cells, HeLa cells, and breast cancer cells, and its nuclear concentration increased after treatment of those cells with leptomycin B, an inhibitor of the exportin Crm1. leptomycin B 244-256 exportin 1 Homo sapiens 287-291 16324157-7 2005 Since GAPDH is known to comprise a CRM1-mediated nuclear export signal, we blocked the nuclear export of GAPDH by treatment with leptomycin B, an inhibitor of CRM1-mediated nuclear export. leptomycin B 129-141 exportin 1 Homo sapiens 35-39 16324157-7 2005 Since GAPDH is known to comprise a CRM1-mediated nuclear export signal, we blocked the nuclear export of GAPDH by treatment with leptomycin B, an inhibitor of CRM1-mediated nuclear export. leptomycin B 129-141 exportin 1 Homo sapiens 159-163 16506698-1 2005 Leptomycin B (LMB) is a Streptomyces metabolite that inhibits the chromosomal region maintenance (CRM)1-dependent nuclear export of proteins. leptomycin B 0-12 exportin 1 Homo sapiens 98-103 16506698-1 2005 Leptomycin B (LMB) is a Streptomyces metabolite that inhibits the chromosomal region maintenance (CRM)1-dependent nuclear export of proteins. leptomycin B 14-17 exportin 1 Homo sapiens 98-103 16140268-6 2005 Moreover, nuclear retention of STAT3 RA by a chromosomal region maintenance 1 (CRM1) inhibitor, leptomycin B, decreased the enhanced STAT3 activation by stimuli. leptomycin B 96-108 exportin 1 Homo sapiens 79-83 15814898-5 2005 Unlike the other family members, the expression of a ZFP36L3-green fluorescent protein fusion protein was entirely cytoplasmic when expressed in 293 cells, even in the presence of the CRM1-dependent nuclear export inhibitor leptomycin B. leptomycin B 224-236 exportin 1 Homo sapiens 184-188 16133873-7 2005 Leptomycin B, an inhibitor of Crm1-mediated nuclear export, decreased the level of FKHRL1 phosphorylation in the presence of IGF-I in vector and FKHR overexpressing cells, but had no effect on the phosphorylation status of FKHR. leptomycin B 0-12 exportin 1 Homo sapiens 30-34 16037817-5 2005 Interestingly, inhibition of exportin Crm1/Xpo1 with leptomycin B strongly alters processing of the 5"-external transcribed spacer, upstream of nuclear export, and reveals a new cleavage site in this transcribed spacer. leptomycin B 53-65 exportin 1 Homo sapiens 38-42 16037817-5 2005 Interestingly, inhibition of exportin Crm1/Xpo1 with leptomycin B strongly alters processing of the 5"-external transcribed spacer, upstream of nuclear export, and reveals a new cleavage site in this transcribed spacer. leptomycin B 53-65 exportin 1 Homo sapiens 43-47 15917227-6 2005 The enhanced green fluorescent protein-tagged ZIP domain of Nrf2 (amino acids 503-589) exhibited a CRM1-dependent cytosolic distribution that could be abrogated by site-directed mutations or treatment with the nuclear export inhibitor, leptomycin B. leptomycin B 236-248 exportin 1 Homo sapiens 99-103 15741163-10 2005 6) Of note, treatment of proliferative Caco-2/15 cells with (R)-roscovitine or leptomycin (an inhibitor of nuclear export through CRM1) led to an accumulation of CDX2 into the nucleus. leptomycin B 79-89 exportin 1 Homo sapiens 130-134 16041368-4 2005 NPM contains a functional nuclear export signal (NES) that is responsible for both its nucleocytoplasmic shuttling and its association with centrosomes, which are Ran-Crm1-dependent as they are sensitive to Crm1-specific nuclear export inhibition, either by leptomycin B (LMB) or by the expression of a Ran-binding protein, RanBP1. leptomycin B 258-270 exportin 1 Homo sapiens 207-211 16041368-4 2005 NPM contains a functional nuclear export signal (NES) that is responsible for both its nucleocytoplasmic shuttling and its association with centrosomes, which are Ran-Crm1-dependent as they are sensitive to Crm1-specific nuclear export inhibition, either by leptomycin B (LMB) or by the expression of a Ran-binding protein, RanBP1. leptomycin B 272-275 exportin 1 Homo sapiens 207-211 15855159-6 2005 Nuclear localization of IEX-1 is also enhanced upon treatment of cells with leptomycin B, an inhibitor of the nuclear exporter CRM1. leptomycin B 76-88 exportin 1 Homo sapiens 127-131 15899855-4 2005 Mutation of the hydrophobic amino acids resulted in nuclear accumulation of both Keap1 and Nrf2, as did treatment with the drug leptomycin B, which inactivates Crm1/exportin. leptomycin B 128-140 exportin 1 Homo sapiens 160-164 15790592-4 2005 After treatment with leptomycin B, a specific inhibitor of CRM1-mediated nuclear export, the accumulation of epitope-tagged FANCA in the nucleus increased, whereas FANCC was affected to a lesser extent and FANCG showed no response. leptomycin B 21-33 exportin 1 Homo sapiens 59-63 15798196-8 2005 Leptomycin B abolished its cytoplasmic accumulation, showing CRM1 dependency. leptomycin B 0-12 exportin 1 Homo sapiens 61-65 15780878-7 2005 In the presence of Leptomycin B, P is retained in the nucleus indicating that it contains a CRM1-dependent nuclear export signal (NES). leptomycin B 19-31 exportin 1 Homo sapiens 92-96 15735731-6 2005 Treatment with Leptomycin B (LMB) blocked the translocation of p27Kip1 to the cytoplasm and prevented its degradation, indicating that CRM1-dependent nuclear export is required for p27Kip1 degradation. leptomycin B 15-27 exportin 1 Homo sapiens 135-139 15735731-6 2005 Treatment with Leptomycin B (LMB) blocked the translocation of p27Kip1 to the cytoplasm and prevented its degradation, indicating that CRM1-dependent nuclear export is required for p27Kip1 degradation. leptomycin B 29-32 exportin 1 Homo sapiens 135-139 15331747-5 2004 Nuclear export of vRNPs representing all of the mutant viruses was completely inhibited in the presence of a CRM1 inhibitor, leptomycin B, as was the transport of wild-type virus, indicating that the CRM1-mediated pathway is responsible for the nuclear export of both wild-type and mutant vRNPs. leptomycin B 125-137 exportin 1 Homo sapiens 109-113 15752138-3 2005 T-plastin shows nuclear accumulation upon incubation of cells with the CRM1 antagonist leptomycin B (LMB). leptomycin B 87-99 exportin 1 Homo sapiens 71-75 15752138-3 2005 T-plastin shows nuclear accumulation upon incubation of cells with the CRM1 antagonist leptomycin B (LMB). leptomycin B 101-104 exportin 1 Homo sapiens 71-75 16128000-6 2005 Interestingly, inhibition of the chromosome region maintenance 1 (CRM1) exportin by leptomycin B (LMB) led to accumulation of alphaNAC in the nucleus. leptomycin B 84-96 exportin 1 Homo sapiens 66-70 16128000-6 2005 Interestingly, inhibition of the chromosome region maintenance 1 (CRM1) exportin by leptomycin B (LMB) led to accumulation of alphaNAC in the nucleus. leptomycin B 98-101 exportin 1 Homo sapiens 66-70 15632073-5 2005 Interestingly, we found that the inhibitory effect of leptomycin B on this CRM1-cargo interaction is Ran dependent. leptomycin B 54-66 exportin 1 Homo sapiens 75-79 15574331-4 2004 Residues critical for NES recognition are identified adjacent to the cysteine residue targeted by leptomycin B (LMB), a specific CRM1 inhibitor. leptomycin B 98-110 exportin 1 Homo sapiens 129-133 15574331-4 2004 Residues critical for NES recognition are identified adjacent to the cysteine residue targeted by leptomycin B (LMB), a specific CRM1 inhibitor. leptomycin B 112-115 exportin 1 Homo sapiens 129-133 15175343-7 2004 STAT2 export could be inhibited with leptomycin B, indicating a nuclear export signal within STAT2 is recognized by the CRM1 exportin carrier. leptomycin B 37-49 exportin 1 Homo sapiens 120-124 15331747-5 2004 Nuclear export of vRNPs representing all of the mutant viruses was completely inhibited in the presence of a CRM1 inhibitor, leptomycin B, as was the transport of wild-type virus, indicating that the CRM1-mediated pathway is responsible for the nuclear export of both wild-type and mutant vRNPs. leptomycin B 125-137 exportin 1 Homo sapiens 200-204 15128734-3 2004 This interaction was inhibited by leptomycin B, a specific inhibitor of CRM1-dependent nuclear export. leptomycin B 34-46 exportin 1 Homo sapiens 72-76 15173319-8 2004 Export by both signals was blocked by treatment of the cells with leptomycin B, indicating that a CRM-1-dependent pathway mediates export. leptomycin B 66-78 exportin 1 Homo sapiens 98-103 15126627-7 2004 Leptomycin B sensitivity experiments revealed that the cytoplasmic expression of IFN-alpha1 mRNA was arrested upon inhibition of CRM1. leptomycin B 0-12 exportin 1 Homo sapiens 129-133 15084399-4 2004 The nuclear export of YY1 was blocked by leptomycin B, an inhibitor of the Crm1-dependent export system. leptomycin B 41-53 exportin 1 Homo sapiens 75-79 15189895-6 2004 The accuracy and consistency of our algorithm was further illustrated by measuring, for the first time in live cells, the dissociation rate (k(d)) of the HIV-1 Rev/CRM1 export complex induced by the cytotoxin leptomycin B. leptomycin B 209-221 exportin 1 Homo sapiens 164-168 15189895-7 2004 Rev/CRM1 colocalization in nucleoli dropped exponentially after addition of leptomycin B at a rate of 1.25 x 10(-3) s(-1). leptomycin B 76-88 exportin 1 Homo sapiens 4-8 15039997-11 2004 Treatment with leptomycin B (LMB) inhibits the translocation of Mre11, Rad50, and Nbs1 to the cytoplasm, leading us to speculate that the relocalization of the proteins to the cytoplasm occurs via CRM1-mediated nuclear export. leptomycin B 15-27 exportin 1 Homo sapiens 197-201 15039997-11 2004 Treatment with leptomycin B (LMB) inhibits the translocation of Mre11, Rad50, and Nbs1 to the cytoplasm, leading us to speculate that the relocalization of the proteins to the cytoplasm occurs via CRM1-mediated nuclear export. leptomycin B 29-32 exportin 1 Homo sapiens 197-201 15051498-6 2004 We found that leptomycin B (LMB) inhibited nuclear export of GFP-alpha-catenin in HCT116 colon cancer cells, suggesting that alpha-catenin localization is regulated by CRM-1-dependent nuclear export. leptomycin B 14-26 exportin 1 Homo sapiens 168-173 15051498-6 2004 We found that leptomycin B (LMB) inhibited nuclear export of GFP-alpha-catenin in HCT116 colon cancer cells, suggesting that alpha-catenin localization is regulated by CRM-1-dependent nuclear export. leptomycin B 28-31 exportin 1 Homo sapiens 168-173 14684748-7 2004 The cytoplasmic localization of Hsc54 was impaired by a mutation in NES as well as by a nuclear export inhibitor, leptomycin B, suggesting that Hsc54 is actively exported from the nucleus to the cytoplasm through a CRM1-dependent mechanism. leptomycin B 114-126 exportin 1 Homo sapiens 215-219 15003534-6 2004 Treatment with leptomycin B, a potent inhibitor of CRM1/exportin1, disrupted the cytoplasmic localization of both Flag-tagged CDC25B and NES-fused GFP. leptomycin B 15-27 exportin 1 Homo sapiens 51-55 15003534-6 2004 Treatment with leptomycin B, a potent inhibitor of CRM1/exportin1, disrupted the cytoplasmic localization of both Flag-tagged CDC25B and NES-fused GFP. leptomycin B 15-27 exportin 1 Homo sapiens 56-65 14729678-5 2004 The sensitivity of subcellular trafficking of these mutants to leptomycin B (LMB), a specific inhibitor of CRM1, was assessed by fluorescence microscopy. leptomycin B 63-75 exportin 1 Homo sapiens 107-111 14729678-5 2004 The sensitivity of subcellular trafficking of these mutants to leptomycin B (LMB), a specific inhibitor of CRM1, was assessed by fluorescence microscopy. leptomycin B 77-80 exportin 1 Homo sapiens 107-111 14996909-9 2004 The 14-3-3 proteins are exported from the nucleus at least in part by a Crm1-dependent, leptomycin B-sensitive mechanism. leptomycin B 88-100 exportin 1 Homo sapiens 72-76 12896982-6 2003 ACF relocates to the cytoplasm after actinomycin D treatment, an effect blocked by the CRM1 inhibitor leptomycin B. leptomycin B 102-114 exportin 1 Homo sapiens 87-91 14996936-15 2004 Furthermore, inhibition of CRM-1-mediated nuclear export by leptomycin B partially prevented the depolarization-induced nuclear efflux of GFP-Sam68. leptomycin B 60-72 exportin 1 Homo sapiens 27-32 14575709-5 2003 Furthermore, upon treatment with leptomycin B, a specific inhibitor of the nuclear export receptor CRM1, a marked nuclear accumulation of hSPHK1 was observed, indicating that hSPHK1 shuttles between the cytoplasm and the nucleus. leptomycin B 33-45 exportin 1 Homo sapiens 99-103 12904306-6 2003 Under conditions of G1 blockade, p27Kip1 was stabilized by inhibition of CRM1-dependent nuclear export with leptomycin B or by mutation of p27Kip1 (Ser10 --> Ala; S10A) interfering with CRM1/p27Kip1 interaction. leptomycin B 108-120 exportin 1 Homo sapiens 73-77 14592774-4 2003 We treated infected cells with the cytotoxin leptomycin B to inhibit CRM1-mediated export; treatment initially delays the onset of late gene expression, but this activity completely recovers as the late phase progresses. leptomycin B 45-57 exportin 1 Homo sapiens 69-73 12874183-9 2003 Leptomycin B, an inhibitor of Crm1 that results in ERK nuclear accumulation, similarly increased p21Waf1/Cip1. leptomycin B 0-12 exportin 1 Homo sapiens 30-34 12917326-8 2003 Leptomycin B, a specific inhibitor of nuclear export receptor CRM1, was found to reverse the cytoplasmic sequestration of HSF1 mediated by 14-3-3 epsilon, suggesting that CRM1/14-3-3 epsilon directed nuclear export plays a major role in repression of HSF1 by the ERK/GSK3/14-3-3 epsilon pathway. leptomycin B 0-12 exportin 1 Homo sapiens 62-66 12917326-8 2003 Leptomycin B, a specific inhibitor of nuclear export receptor CRM1, was found to reverse the cytoplasmic sequestration of HSF1 mediated by 14-3-3 epsilon, suggesting that CRM1/14-3-3 epsilon directed nuclear export plays a major role in repression of HSF1 by the ERK/GSK3/14-3-3 epsilon pathway. leptomycin B 0-12 exportin 1 Homo sapiens 171-175 12807744-5 2003 Caffeine inhibited the accumulation of p53 induced by leptomycin B (LMB), an inhibitor of CRM1, but not N-acetyl-leu-leu-norleucinal, a proteasome inhibitor. leptomycin B 54-66 exportin 1 Homo sapiens 90-94 12807744-5 2003 Caffeine inhibited the accumulation of p53 induced by leptomycin B (LMB), an inhibitor of CRM1, but not N-acetyl-leu-leu-norleucinal, a proteasome inhibitor. leptomycin B 68-71 exportin 1 Homo sapiens 90-94 12468543-0 2003 Leptomycin B, an inhibitor of the nuclear export receptor CRM1, inhibits COX-2 expression. leptomycin B 0-12 exportin 1 Homo sapiens 58-62 12749860-8 2003 Like LMB, it seems that the ratjadones covalently bind to CRM1, inhibit cargo protein binding via LR-NES, and thereby block nuclear export. leptomycin B 5-8 exportin 1 Homo sapiens 58-62 12678777-8 2003 LMB binds directly to CRM1, which results in dissociation of the NES from the nuclear export machinery containing CRM1. leptomycin B 0-3 exportin 1 Homo sapiens 22-26 12678777-8 2003 LMB binds directly to CRM1, which results in dissociation of the NES from the nuclear export machinery containing CRM1. leptomycin B 0-3 exportin 1 Homo sapiens 114-118 12519765-5 2003 In transfected cells, the Smurf1-Smad7 complex was accumulated in the cytoplasm by the nuclear export receptor, CRM1; this action was prevented by treatment with leptomycin B, a specific inactivator of CRM1 function. leptomycin B 162-174 exportin 1 Homo sapiens 112-116 12519765-5 2003 In transfected cells, the Smurf1-Smad7 complex was accumulated in the cytoplasm by the nuclear export receptor, CRM1; this action was prevented by treatment with leptomycin B, a specific inactivator of CRM1 function. leptomycin B 162-174 exportin 1 Homo sapiens 202-206 12724356-8 2003 Indeed, we observed that nuclear export of human NMD3 (hNMD3) is sensitive to leptomycin B (LMB), which inactivates CRM1. leptomycin B 78-90 exportin 1 Homo sapiens 116-120 12724356-8 2003 Indeed, we observed that nuclear export of human NMD3 (hNMD3) is sensitive to leptomycin B (LMB), which inactivates CRM1. leptomycin B 92-95 exportin 1 Homo sapiens 116-120 12670929-5 2003 The results reported herein indicate that Tax contains a leucine-rich nuclear export signal (NES) that, when fused to green fluorescent protein (GFP), can direct nuclear export via the CRM-1 pathway, as determined by leptomycin B inhibition of nuclear export. leptomycin B 217-229 exportin 1 Homo sapiens 185-190 12637523-6 2003 Moreover, 69-kDa choline acetyltransferase but not the 82-kDa enzyme is transported out of the nucleus by the leptomycin B-sensitive Crm-1 export pathway. leptomycin B 110-122 exportin 1 Homo sapiens 133-138 12424202-4 2003 Treatment of both cell types with leptomycin B, an inhibitor of CRM1-dependent cyclin B1 nuclear export, induces apoptosis. leptomycin B 34-46 exportin 1 Homo sapiens 64-68 12468543-3 2003 Here, we report that leptomycin B (LMB), a specific inhibitor of the nuclear export factor CRM1 potently inhibits the stabilization of COX-2 mRNA in MDA-MB-231 human mammary cancer cells. leptomycin B 21-33 exportin 1 Homo sapiens 91-95 12468543-3 2003 Here, we report that leptomycin B (LMB), a specific inhibitor of the nuclear export factor CRM1 potently inhibits the stabilization of COX-2 mRNA in MDA-MB-231 human mammary cancer cells. leptomycin B 35-38 exportin 1 Homo sapiens 91-95 12239324-6 2002 When cells were treated with the CRM1 inhibitor leptomycin B in the presence of Rev protein, the env intron containing HIV RNAs formed clusters throughout the nucleoplasm and accumulated at the nuclear pores. leptomycin B 48-60 exportin 1 Homo sapiens 33-37 12384512-8 2002 MST1 is predominantly cytoplasmic, but cycles continuously through the nucleus, as evidenced by its rapid accumulation in the nucleus after addition of the Crm1 inhibitor, leptomycin B. leptomycin B 172-184 exportin 1 Homo sapiens 156-160 12438613-3 2002 ICP27 export was reported to be sensitive to the CRM1 inhibitor leptomycin B (LMB) in HSV-1-infected cells but not in Xenopus oocytes, where ICP27 interacts with the export factor Aly/REF to access the TAP export pathway. leptomycin B 64-76 exportin 1 Homo sapiens 49-53 12438613-3 2002 ICP27 export was reported to be sensitive to the CRM1 inhibitor leptomycin B (LMB) in HSV-1-infected cells but not in Xenopus oocytes, where ICP27 interacts with the export factor Aly/REF to access the TAP export pathway. leptomycin B 78-81 exportin 1 Homo sapiens 49-53 12360403-6 2002 The HPIP C-terminal domain (aa 443-731) bears a nuclear export activity that is blocked by the CRM1 inhibitor Leptomycin B. leptomycin B 110-122 exportin 1 Homo sapiens 95-99 12374846-9 2002 These effects are similar to those of the known CRM1 inhibitor leptomycin B and suggest that the inhibitory effect of the compound is caused by binding to CRM1 at a similar site. leptomycin B 63-75 exportin 1 Homo sapiens 48-52 12374846-9 2002 These effects are similar to those of the known CRM1 inhibitor leptomycin B and suggest that the inhibitory effect of the compound is caused by binding to CRM1 at a similar site. leptomycin B 63-75 exportin 1 Homo sapiens 155-159 12138193-6 2002 Mutational inactivation of this nuclear export signal or treatment of cells with the CRM1-specific export inhibitor leptomycin B inhibited nuclear export and consequently nucleocytoplasmic shuttling of Sox10. leptomycin B 116-128 exportin 1 Homo sapiens 85-89 12217955-12 2002 Deletion of this NES or treatment with the CRM1-specific inhibitor leptomycin B leads to overall nuclear accumulation of merlin isoforms missing exon 2. leptomycin B 67-79 exportin 1 Homo sapiens 43-47 12193389-4 2002 A series of embryo culture experiments that employed a specific inhibitor of CRM1, leptomycin B, indicated that CRM1 function is not required for development until after the 4-cell stage of porcine embryo development. leptomycin B 83-95 exportin 1 Homo sapiens 77-81 12193389-4 2002 A series of embryo culture experiments that employed a specific inhibitor of CRM1, leptomycin B, indicated that CRM1 function is not required for development until after the 4-cell stage of porcine embryo development. leptomycin B 83-95 exportin 1 Homo sapiens 112-116 12145331-12 2002 Treatment with leptomycin B, an inhibitor of CRM-1 receptor-mediated export, inhibited export of unliganded GFP-VDR but did not influence export of liganded GFP-VDR or YFP-RXR. leptomycin B 15-27 exportin 1 Homo sapiens 45-50 12071851-6 2002 Leptomycin B is an inhibitor of CRM1/exportin-1, and reversed the ceramide-induced decrease in nuclear C/EBPbeta at 24 h. Taken together, these data support the hypothesis that ceramide may inhibit adipogenesis, at least in part, by enhancing dephosphorylation and premature nuclear export of C/EBPbeta at a time when its maximal transcriptional activity is required to drive adipogenesis. leptomycin B 0-12 exportin 1 Homo sapiens 32-36 12071851-6 2002 Leptomycin B is an inhibitor of CRM1/exportin-1, and reversed the ceramide-induced decrease in nuclear C/EBPbeta at 24 h. Taken together, these data support the hypothesis that ceramide may inhibit adipogenesis, at least in part, by enhancing dephosphorylation and premature nuclear export of C/EBPbeta at a time when its maximal transcriptional activity is required to drive adipogenesis. leptomycin B 0-12 exportin 1 Homo sapiens 37-47 11889117-4 2002 The p27(Kip1) protein was translocated from the nucleus to the cytoplasm at the G(0)-G(1) transition of the cell cycle, and this export was inhibited by leptomycin B, a specific inhibitor of CRM1-dependent nuclear export. leptomycin B 153-165 exportin 1 Homo sapiens 191-195 11781313-6 2002 The PTB NES cannot be shown to bind to CAS or Crm1, cellular receptors known to export proteins from the nucleus, and it functions in the presence of leptomycin B, a specific inhibitor of Crm1-dependent export. leptomycin B 150-162 exportin 1 Homo sapiens 188-192 11891341-5 2002 We also found that Gag is transported out of the nucleus through the CRM1 nuclear export pathway, based on observations that treatment of virus-expressing cells with leptomycin B resulted in the redistribution of Gag proteins from the cytoplasm to the nucleus. leptomycin B 166-178 exportin 1 Homo sapiens 69-73 11884514-6 2002 When cells were treated with the CRM-1-dependent nuclear export inhibitor, leptomycin B (LMB), there was nuclear accumulation of IkappaBalpha-EGFP and p65-dsRed, with IkappaBalpha-EGFP accumulating more rapidly. leptomycin B 75-87 exportin 1 Homo sapiens 33-38 11884514-6 2002 When cells were treated with the CRM-1-dependent nuclear export inhibitor, leptomycin B (LMB), there was nuclear accumulation of IkappaBalpha-EGFP and p65-dsRed, with IkappaBalpha-EGFP accumulating more rapidly. leptomycin B 89-92 exportin 1 Homo sapiens 33-38 11741900-8 2002 The increase in Rev activity was sensitive to leptomycin B, but not to olomoucine, indicating that the effect of SLM-1, SLM-2, QKI-5, QKI-6, and QKI-7 is exerted through a CRM-1-dependent mRNA export pathway. leptomycin B 46-58 exportin 1 Homo sapiens 172-177 11604520-4 2001 Mutation of the nuclear export signal or treatment with the CRM1-specific drug leptomycin B induces nuclear accumulation of (i)ADAR1 fused to the green fluorescent protein and increases the nuclear editing activity. leptomycin B 79-91 exportin 1 Homo sapiens 60-64 11741955-5 2002 To determine whether intact KSR is normally imported to the nucleus, REF-52 fibroblasts expressing KSR were treated with 10 nm leptomycin B, which inhibits Crm1-dependent nuclear export. leptomycin B 127-139 exportin 1 Homo sapiens 156-160 11704659-2 2002 Nuclear-cytoplasmic translocation plays an important role because leptomycin B (LMB), a chemical inhibitor of CRM1-dependent nuclear export, prevents p27 degradation mediated by Jab1/CSN5. leptomycin B 66-78 exportin 1 Homo sapiens 110-114 11704659-2 2002 Nuclear-cytoplasmic translocation plays an important role because leptomycin B (LMB), a chemical inhibitor of CRM1-dependent nuclear export, prevents p27 degradation mediated by Jab1/CSN5. leptomycin B 80-83 exportin 1 Homo sapiens 110-114 11704659-4 2002 Jab1/CSN5, but not p27, contains a typical leucine-rich nuclear export signal (NES) sequence conserved among different species, through which CRM1 bound to Jab1/CSN5 in an LMB-sensitive manner. leptomycin B 172-175 exportin 1 Homo sapiens 142-146 11726507-5 2001 The cytoplasmic localization and association of C/EBPbeta-PSer239 with CRM1 (exportin-1) in TNF-alpha-treated hepatocytes was inhibited by leptomycin B, a blocker of CRM1 activity. leptomycin B 139-151 exportin 1 Homo sapiens 71-75 11726507-5 2001 The cytoplasmic localization and association of C/EBPbeta-PSer239 with CRM1 (exportin-1) in TNF-alpha-treated hepatocytes was inhibited by leptomycin B, a blocker of CRM1 activity. leptomycin B 139-151 exportin 1 Homo sapiens 77-87 11726507-5 2001 The cytoplasmic localization and association of C/EBPbeta-PSer239 with CRM1 (exportin-1) in TNF-alpha-treated hepatocytes was inhibited by leptomycin B, a blocker of CRM1 activity. leptomycin B 139-151 exportin 1 Homo sapiens 166-170 11567023-8 2001 TAF(II)105 nuclear export signal is leptomycin B-resistant indicating that it belongs to a CRM1-independent nuclear export pathway. leptomycin B 36-48 exportin 1 Homo sapiens 91-95 11733048-4 2001 Here we use fluorescence recovery after photobleaching for the first time to show that, in living cells, parathyroid hormone-related protein is exported from the nucleus in a leptomycin B-sensitive manner, implicating CRM1 as the parathyroid hormone-related protein nuclear export receptor. leptomycin B 175-187 exportin 1 Homo sapiens 218-222 11337505-6 2001 Under similar conditions, nuclear c-ABL was only exported after the addition of cytosolic extract, and the export was blocked by the CRM1-specific inhibitor, leptomycin B. leptomycin B 158-170 exportin 1 Homo sapiens 133-137 11565755-7 2001 CRM1 coimmunoprecipitates with HuR only after heat shock, and nuclear export of HuR becomes sensitive to leptomycin B, an inhibitor of CRM1. leptomycin B 105-117 exportin 1 Homo sapiens 135-139 11382772-8 2001 Leptomycin B, an inhibitor for Crm1, increased basal nuclear GATA4 and suppressed GSK3beta-induced decreases in nuclear GATA4. leptomycin B 0-12 exportin 1 Homo sapiens 31-35 11477086-4 2001 This effect of Su(fu) overexpression can be blocked by treatment of these cells with leptomycin B, a specific inhibitor of CRM1-mediated nuclear export. leptomycin B 85-97 exportin 1 Homo sapiens 123-127 11543664-7 2001 Cytoplasmic accumulation of both the aa 225-237 and 218-227 containing reporters was minimal in the presence of the inhibitor leptomycin B, indicating that both motifs mediated Crm-1-dependent export. leptomycin B 126-138 exportin 1 Homo sapiens 177-182 11306562-7 2001 Export from the nucleus was inhibited by leptomycin B, suggesting the involvement of the nuclear export protein CRM1. leptomycin B 41-53 exportin 1 Homo sapiens 112-116 11375905-7 2001 Both lactacystin, a specific inhibitor of the 26S proteasome, and leptomycin B, a specific inhibitor of the nuclear export protein CRM1, could block the effect that DCA had on p53 protein levels, suggesting that DCA suppressed p53 by stimulating the process of proteasome-mediated degradation of p53. leptomycin B 66-78 exportin 1 Homo sapiens 131-135 11451992-8 2001 Furthermore, nuclear accumulation of human (hs) ADAR1 is transcription dependent and can be stimulated by LMB, an inhibitor of Crm1-dependent nuclear export, indicating that hsADAR1 can move between the nucleus and cytoplasm. leptomycin B 106-109 exportin 1 Homo sapiens 127-131 11287420-4 2001 We found that the predominant cytoplasmic localization of HBx is sensitive to the drug leptomycin B (LMB), which specifically inactivates Crm1. leptomycin B 87-99 exportin 1 Homo sapiens 138-142 11287420-4 2001 We found that the predominant cytoplasmic localization of HBx is sensitive to the drug leptomycin B (LMB), which specifically inactivates Crm1. leptomycin B 101-104 exportin 1 Homo sapiens 138-142 11114166-4 2000 By using leptomycin B (LMB), a specific inhibitor of nuclear export receptor Crm1, we show that FRAP is a cytoplasmic-nuclear shuttling protein. leptomycin B 23-26 exportin 1 Homo sapiens 77-81 11115515-4 2001 Leptomycin B treatment induced rapid nuclear accumulation of GFP-lambda as well as endogenous lambdaPKC suggesting the existence of a CRM1-dependent nuclear export signal (NES). leptomycin B 0-12 exportin 1 Homo sapiens 134-138 11266504-11 2001 In contrast to TR alpha, nuclear export of its oncogenic homolog v-ErbA is sensitive to leptomycin B, suggesting that the oncoprotein follows a CRM1-mediated export pathway. leptomycin B 88-100 exportin 1 Homo sapiens 144-148 11152524-5 2001 However, the addition of leptomycin B (LMB), a potent inhibitor of CRM1-dependent nuclear export, produces a shift from a cytoplasmic localization to a nuclear pattern, suggesting that these Vpr fusion proteins shuttle into and out of the nucleus. leptomycin B 25-37 exportin 1 Homo sapiens 67-71 11152524-5 2001 However, the addition of leptomycin B (LMB), a potent inhibitor of CRM1-dependent nuclear export, produces a shift from a cytoplasmic localization to a nuclear pattern, suggesting that these Vpr fusion proteins shuttle into and out of the nucleus. leptomycin B 39-42 exportin 1 Homo sapiens 67-71 11134317-1 2001 Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. leptomycin B 0-12 exportin 1 Homo sapiens 46-50 11134317-1 2001 Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. leptomycin B 14-17 exportin 1 Homo sapiens 46-50 11336797-2 2001 However, in this report, we demonstrate that Trip6 is largely in the nucleus in cells treated with leptomycin B, suggesting that Trip6 shuttles between nuclear and cytoplasmic compartments and that nuclear export of Trip6 is dependent on Crm1. leptomycin B 99-111 exportin 1 Homo sapiens 238-242 11309306-4 2001 Inhibition of CRM1-dependent nuclear export with leptomycin B or mutation of the nuclear export signal motif of Beclin 1 results in predominantly nuclear localization. leptomycin B 49-61 exportin 1 Homo sapiens 14-18 11162521-5 2001 Leptomycin B completely inhibited Pi-induced nuclear export, suggesting that CRM1/exportin 1 is involved in Pi-induced nuclear export. leptomycin B 0-12 exportin 1 Homo sapiens 77-81 11162521-5 2001 Leptomycin B completely inhibited Pi-induced nuclear export, suggesting that CRM1/exportin 1 is involved in Pi-induced nuclear export. leptomycin B 0-12 exportin 1 Homo sapiens 82-92 11016936-8 2000 This nucleocytoplasmic shuttling is sensitive to the exportin 1/CRM1-inactivating drug, leptomycin B, indicating that Btk utilizes functional nuclear export signals. leptomycin B 88-100 exportin 1 Homo sapiens 53-63 11016936-8 2000 This nucleocytoplasmic shuttling is sensitive to the exportin 1/CRM1-inactivating drug, leptomycin B, indicating that Btk utilizes functional nuclear export signals. leptomycin B 88-100 exportin 1 Homo sapiens 64-68 11114166-4 2000 By using leptomycin B (LMB), a specific inhibitor of nuclear export receptor Crm1, we show that FRAP is a cytoplasmic-nuclear shuttling protein. leptomycin B 9-21 exportin 1 Homo sapiens 77-81 10991937-7 2000 Mutational inactivation of this nuclear export sequence, or treatment of cells with the CRM1-specific export inhibitor leptomycin B, induced nuclear accumulation of ectopic full-length BRCA1. leptomycin B 119-131 exportin 1 Homo sapiens 88-92 11018049-7 2000 The inhibition of CRM1 by leptomycin B leads to the nuclear retention of pp32 and APRIL, their increased association with HuR, and an increase in HuR"s association with nuclear poly(A)+ RNA. leptomycin B 26-38 exportin 1 Homo sapiens 18-22 11035805-8 2000 Moreover, endogenous APC accumulated in the nucleus of cells treated with the Crm1-specific nuclear export inhibitor leptomycin B. leptomycin B 117-129 exportin 1 Homo sapiens 78-82 11074002-7 2000 We demonstrate that inhibition of CRM1-mediated nuclear export by treatment of cells with leptomycin B results in endogenous Smad4 accumulating very rapidly in the nucleus. leptomycin B 90-102 exportin 1 Homo sapiens 34-38 11044131-3 2000 Leptomycin B inhibitor studies revealed that the RNAs derived from the codon-optimized gag gene lacking AU-rich inhibitory elements are directed to a distinct, CRM1-independent, nuclear export pathway. leptomycin B 0-12 exportin 1 Homo sapiens 160-164 10980707-6 2000 Treatment of cells with the CRM1-specific export inhibitor leptomycin B shifts APC from cytoplasm to nucleus. leptomycin B 59-71 exportin 1 Homo sapiens 28-32 11000220-5 2000 This RNA export pathway is CRM1 dependent and can be blocked by the cytotoxic agent leptomycin B. leptomycin B 84-96 exportin 1 Homo sapiens 27-31 10846090-5 2000 Accordingly, leptomycin B (LMB), a specific Crm-1 inhibitor, impairs Rev- but not EB2-dependent nuclear export of unspliced RNA. leptomycin B 13-25 exportin 1 Homo sapiens 44-49 10846090-5 2000 Accordingly, leptomycin B (LMB), a specific Crm-1 inhibitor, impairs Rev- but not EB2-dependent nuclear export of unspliced RNA. leptomycin B 27-30 exportin 1 Homo sapiens 44-49 10801471-8 2000 The recognition domain of CRM1 has been mapped and includes residues found previously to affect binding of leptomycin B, a fungicide interfering with nuclear export. leptomycin B 107-119 exportin 1 Homo sapiens 26-30 10809773-5 2000 The CLS directed leptomycin B-sensitive nuclear export of reporter proteins, suggesting its dependence on the nuclear exporter Crm1/exportin 1. leptomycin B 17-29 exportin 1 Homo sapiens 127-131 10809773-5 2000 The CLS directed leptomycin B-sensitive nuclear export of reporter proteins, suggesting its dependence on the nuclear exporter Crm1/exportin 1. leptomycin B 17-29 exportin 1 Homo sapiens 132-142 10788511-8 2000 In addition, the inhibition of the nuclear accumulation of NFATp by p38 appears to be mediated through the activation of NFATp nuclear export and takes place in a Leptomycin B-sensitive fashion, suggesting the involvement of the exportin CRM1 in this process. leptomycin B 163-175 exportin 1 Homo sapiens 238-242 10772978-8 2000 Furthermore this cooperation was shown to remain susceptible to the effect of leptomycin B, which blocks the binding of hCRM1 to the nuclear export signal of Rev. leptomycin B 78-90 exportin 1 Homo sapiens 120-125 10688673-8 2000 In mammalian cells, inhibition of CRM1 by leptomycin B resulted in nuclear localization of cotransfected p65 and IkappaBalpha in COS cells and enhanced nuclear relocation of endogenous p65 in T cells. leptomycin B 42-54 exportin 1 Homo sapiens 34-38 10739668-7 2000 All of the Rev-type NESs were inactivated by mutation of key hydrophobic residues and by treatment with the CRM1-specific export inhibitor, leptomycin B. leptomycin B 140-152 exportin 1 Homo sapiens 108-112 10655476-6 2000 Importantly, a LMB-insensitive CRM1 mutant (Crm1-K1) abolished LMB-induced nuclear accumulation of the inactive complexes. leptomycin B 15-18 exportin 1 Homo sapiens 44-48 10655476-6 2000 Importantly, a LMB-insensitive CRM1 mutant (Crm1-K1) abolished LMB-induced nuclear accumulation of the inactive complexes. leptomycin B 63-66 exportin 1 Homo sapiens 31-35 10655476-6 2000 Importantly, a LMB-insensitive CRM1 mutant (Crm1-K1) abolished LMB-induced nuclear accumulation of the inactive complexes. leptomycin B 63-66 exportin 1 Homo sapiens 44-48 10567585-9 1999 The export function of NXT1 is blocked by the addition of leptomycin B, a compound that selectively inhibits the NES receptor Crm1. leptomycin B 58-70 exportin 1 Homo sapiens 126-130 10636888-0 2000 A leptomycin B-sensitive homologue of human CRM1 promotes nuclear export of nuclear export sequence-containing proteins in Drosophila cells. leptomycin B 2-14 exportin 1 Homo sapiens 44-48 10636888-7 2000 In particular, we show that Drosophila cells express a leptomycin B-sensitive homologue of human CRM1 that supports Rev-dependent gene expression and is required for nuclear export of NES-containing proteins in insect cells. leptomycin B 55-67 exportin 1 Homo sapiens 97-101 10637295-4 2000 In addition, LPP accumulates in the nucleus of cells upon treatment with leptomycin B, an inhibitor of the export factor CRM1. leptomycin B 73-85 exportin 1 Homo sapiens 121-125 10628746-5 2000 Leucine-rich NESs utilize the exportin 1/CRM1-dependent nuclear export pathway, which can be blocked by leptomycin B (LMB). leptomycin B 104-116 exportin 1 Homo sapiens 30-40 10628746-5 2000 Leucine-rich NESs utilize the exportin 1/CRM1-dependent nuclear export pathway, which can be blocked by leptomycin B (LMB). leptomycin B 104-116 exportin 1 Homo sapiens 41-45 10628746-5 2000 Leucine-rich NESs utilize the exportin 1/CRM1-dependent nuclear export pathway, which can be blocked by leptomycin B (LMB). leptomycin B 118-121 exportin 1 Homo sapiens 30-40 10628746-5 2000 Leucine-rich NESs utilize the exportin 1/CRM1-dependent nuclear export pathway, which can be blocked by leptomycin B (LMB). leptomycin B 118-121 exportin 1 Homo sapiens 41-45 10438867-7 1999 Moreover, we observed that the treatment of MVM-infected cells with leptomycin B, a drug that specifically inhibits the CRM1-dependent nuclear export pathway, leads to a drastic accumulation of NS2 proteins in the nucleus. leptomycin B 68-80 exportin 1 Homo sapiens 120-124 10527855-3 1999 We report here that nuclear export of NS2 is inhibited by leptomycin B (LMB), a drug that specifically blocks nuclear export signal (NES)-chromosomal region maintenance 1 (CRM1) interactions. leptomycin B 58-70 exportin 1 Homo sapiens 172-176 10527855-3 1999 We report here that nuclear export of NS2 is inhibited by leptomycin B (LMB), a drug that specifically blocks nuclear export signal (NES)-chromosomal region maintenance 1 (CRM1) interactions. leptomycin B 72-75 exportin 1 Homo sapiens 172-176 10438867-8 1999 Both NS2 interaction with CRM1 and nuclear accumulation upon leptomycin B treatment strongly suggest that these nonstructural viral proteins are actively exported out of the nuclei of infected cells via a CRM1-mediated nuclear export pathway. leptomycin B 61-73 exportin 1 Homo sapiens 205-209 10371501-3 1999 Differential sensitivity to leptomycin B, an inhibitor of CRM-1, indicates that the export pathways mediated by Rev and Sam68 are distinct. leptomycin B 28-40 exportin 1 Homo sapiens 58-63 10430904-0 1999 Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region. leptomycin B 0-12 exportin 1 Homo sapiens 25-29 10430904-1 1999 The cellular target of leptomycin B (LMB), a nuclear export inhibitor, has been identified as CRM1 (exportin 1), an evolutionarily conserved receptor for the nuclear export signal of proteins. leptomycin B 23-35 exportin 1 Homo sapiens 94-98 10430904-1 1999 The cellular target of leptomycin B (LMB), a nuclear export inhibitor, has been identified as CRM1 (exportin 1), an evolutionarily conserved receptor for the nuclear export signal of proteins. leptomycin B 37-40 exportin 1 Homo sapiens 94-98 10430904-3 1999 CRM1 in a Schizosaccharomyces pombe mutant showing extremely high resistance to LMB had a single amino acid replacement at Cys-529 with Ser. leptomycin B 80-83 exportin 1 Homo sapiens 0-4 10430904-4 1999 The mutant gene, named crm1-K1, conferred LMB resistance on wild-type S. pombe, and Crm1-K1 no longer bound biotinylated LMB. leptomycin B 42-45 exportin 1 Homo sapiens 23-27 10430904-4 1999 The mutant gene, named crm1-K1, conferred LMB resistance on wild-type S. pombe, and Crm1-K1 no longer bound biotinylated LMB. leptomycin B 121-124 exportin 1 Homo sapiens 23-27 10430904-6 1999 When HeLa cells were cultured with biotinylated LMB, the only cellular protein bound covalently was CRM1. leptomycin B 48-51 exportin 1 Homo sapiens 100-104 10430904-7 1999 Inhibition by N-ethylmaleimide (NEM), an alkylating agent, of CRM1-mediated nuclear export probably was caused by covalent binding of the electrophilic structure in NEM to the sulfhydryl group of Cys-529, because the crm1-K1 mutant showed the normal rate for the export of Rev nuclear export signal-bearing proteins in the presence of not only LMB but also NEM. leptomycin B 344-347 exportin 1 Homo sapiens 62-66 10430904-7 1999 Inhibition by N-ethylmaleimide (NEM), an alkylating agent, of CRM1-mediated nuclear export probably was caused by covalent binding of the electrophilic structure in NEM to the sulfhydryl group of Cys-529, because the crm1-K1 mutant showed the normal rate for the export of Rev nuclear export signal-bearing proteins in the presence of not only LMB but also NEM. leptomycin B 344-347 exportin 1 Homo sapiens 217-221 10430904-8 1999 These results show that the single cysteine residue determines LMB sensitivity and is selectively alkylated by LMB, leading to CRM1 inactivation. leptomycin B 63-66 exportin 1 Homo sapiens 127-131 10430904-8 1999 These results show that the single cysteine residue determines LMB sensitivity and is selectively alkylated by LMB, leading to CRM1 inactivation. leptomycin B 111-114 exportin 1 Homo sapiens 127-131 10400785-10 1999 Mutation of a leucine-rich region (LRR) of SM inhibited CRM 1-mediated cytoplasmic translocation and SM activity, as did leptomycin B, an inhibitor of CRM 1 complex formation. leptomycin B 121-133 exportin 1 Homo sapiens 151-156 10421839-3 1999 RESULTS: We have found that treatment of mammalian cells with leptomycin B (LMB), a specific inhibitor of CRM1, induces the nuclear accumulation of endogenous mRNA, probably due to the inhibition of its export. leptomycin B 62-74 exportin 1 Homo sapiens 106-110 10421839-3 1999 RESULTS: We have found that treatment of mammalian cells with leptomycin B (LMB), a specific inhibitor of CRM1, induces the nuclear accumulation of endogenous mRNA, probably due to the inhibition of its export. leptomycin B 76-79 exportin 1 Homo sapiens 106-110 10421839-6 1999 LMB treatment, on the other hand, rapidly induced the nuclear entry of RanBP1, a factor involved in the active nucleocytoplasmic transport, although the treatment did not interfere with a nuclear localization signal-dependent transport system within 7 h. CONCLUSION: These results suggest that CRM1 is involved in mRNA export in eukaryotic cells. leptomycin B 0-3 exportin 1 Homo sapiens 294-298 9891038-7 1999 Nuclear export of GR was inhibited by leptomycin B, suggesting that the transfer of GR to the cytoplasm is mediated through the CRM1-dependent pathway. leptomycin B 38-50 exportin 1 Homo sapiens 128-132 9683540-0 1998 Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1. leptomycin B 0-12 exportin 1 Homo sapiens 79-83 9837918-3 1998 Using purified recombinant factors, we show by co-precipitation, gel mobility shift and protein footprinting assays that full-length Rev protein interacts directly with CRM1 in vitro independently of both the integrity of the characteristic leucine residues of the NES and the presence of the cytotoxin leptomycin B (LMB). leptomycin B 303-315 exportin 1 Homo sapiens 169-173 9837918-3 1998 Using purified recombinant factors, we show by co-precipitation, gel mobility shift and protein footprinting assays that full-length Rev protein interacts directly with CRM1 in vitro independently of both the integrity of the characteristic leucine residues of the NES and the presence of the cytotoxin leptomycin B (LMB). leptomycin B 317-320 exportin 1 Homo sapiens 169-173 9837918-4 1998 Addition of RanGTP induces the formation of an RRE-Rev-CRM1-RanGTP complex that is sensitive to LMB, NES mutations, and Ran being charged with GTP. leptomycin B 96-99 exportin 1 Homo sapiens 55-59 9819415-3 1998 The drug leptomycin B (LMB) blocks the formation of nuclear export complexes consisting of CRM1, RanGTP, and NES-containing proteins. leptomycin B 9-21 exportin 1 Homo sapiens 91-95 9819415-3 1998 The drug leptomycin B (LMB) blocks the formation of nuclear export complexes consisting of CRM1, RanGTP, and NES-containing proteins. leptomycin B 23-26 exportin 1 Homo sapiens 91-95 9696859-4 1998 The function of the atypical NES-containing Rev-like proteins of equine infectious anemia virus (EIAV) and feline immunodeficiency virus (FIV) is inhibited by leptomycin B, a drug that specifically blocks NES-CRM1 interactions. leptomycin B 159-171 exportin 1 Homo sapiens 209-213 9710628-2 1998 We now demonstrate that the c-Rel-IkappaB alpha complex is stably retained in the cytoplasm in the presence of leptomycin B, a specific inhibitor of Crm1-mediated nuclear export. leptomycin B 111-123 exportin 1 Homo sapiens 149-153 9683540-2 1998 Recently, LMB was shown to inhibit the function of CRM1, a receptor for the nuclear export signal (NES). leptomycin B 10-13 exportin 1 Homo sapiens 51-55 9683540-4 1998 Binding experiments with a biotinylated derivative of LMB and a HeLa cell extract led to identifying CRM1 as a major protein that bound to the LMB derivative. leptomycin B 54-57 exportin 1 Homo sapiens 101-105 9683540-4 1998 Binding experiments with a biotinylated derivative of LMB and a HeLa cell extract led to identifying CRM1 as a major protein that bound to the LMB derivative. leptomycin B 143-146 exportin 1 Homo sapiens 101-105 9683540-8 1998 The inhibition of CRM1 by LMB was also observed in fission yeast. leptomycin B 26-29 exportin 1 Homo sapiens 18-22 9683540-10 1998 Interestingly, a protein containing both NES and NLS, which is expected to shuttle between nucleus and cytoplasm, was highly accumulated in the nucleus of the crm1 mutant cells or of cells treated with LMB. leptomycin B 202-205 exportin 1 Homo sapiens 159-163 33589493-4 2021 Nup214 versions with mutations in the NES, as well as wild-type Nup214 in the presence of the selective CRM1 inhibitor leptomycin B, accumulate in the nucleus of Nup214-overexpressing cells. leptomycin B 119-131 exportin 1 Homo sapiens 104-108 9679058-6 1998 In support of this hypothesis, we found that treatment of cells with leptomycin B, which disrupted Cyclin B1-CRM1 interactions, led to a marked nuclear accumulation of Cyclin B1. leptomycin B 69-81 exportin 1 Homo sapiens 109-113 9368044-1 1997 Crm1 of Schizosaccharomyces pombe, a nuclear protein essential for proliferation and chromosome region maintenance, is a possible target of leptomycin B, an antifungal and antitumor antibiotic with cell cycle-arresting activity. leptomycin B 140-152 exportin 1 Homo sapiens 0-4 9670027-5 1998 Cyclin B1 nuclear export is blocked by leptomycin B, an inhibitor of the recently identified export factor, exportin 1 (CRM1). leptomycin B 39-51 exportin 1 Homo sapiens 108-118 9670027-5 1998 Cyclin B1 nuclear export is blocked by leptomycin B, an inhibitor of the recently identified export factor, exportin 1 (CRM1). leptomycin B 39-51 exportin 1 Homo sapiens 120-124 9628873-5 1998 Since leptomycin B, which inhibits the interaction of exportin 1 with the Rev-type leucine-rich nuclear export signal (NES), blocks stress-dependent translocation of GFP-MK2, it is supposed that phosphorylation-induced export of the protein causes the translocation. leptomycin B 6-18 exportin 1 Homo sapiens 54-64 10196376-6 1999 Using leptomycin B we demonstrate that transport of the FXR proteins out of the nucleus is mediated by the export receptor exportin1. leptomycin B 6-18 exportin 1 Homo sapiens 123-132 34271037-6 2021 Using the CRM1 inhibitor leptomycin B (LMB), we demonstrated that CRM1 inhibition promoted AcMNPV replication, as LMB treatment readily increased the virus titer, and even functionally surrogate Ac34 to rescue the infectivity of an ac34-knockout virus. leptomycin B 25-37 exportin 1 Homo sapiens 10-14 34643426-4 2022 NES1 and NES2 are both functional and transferable NESs, and their nuclear export activity is blocked by leptomycin B (LMB), suggesting that the export of NS1 from the nucleus is dependent upon the chromosome region maintenance 1 (CRM1) pathway. leptomycin B 105-117 exportin 1 Homo sapiens 231-235 34643426-4 2022 NES1 and NES2 are both functional and transferable NESs, and their nuclear export activity is blocked by leptomycin B (LMB), suggesting that the export of NS1 from the nucleus is dependent upon the chromosome region maintenance 1 (CRM1) pathway. leptomycin B 119-122 exportin 1 Homo sapiens 231-235 34832913-4 2021 The best inhibitors described so far (leptomycin B and SINE compounds) interact with XPO1 through a covalent interaction with Cys528 located in the NES-binding cleft of XPO1. leptomycin B 38-50 exportin 1 Homo sapiens 85-89 34832913-4 2021 The best inhibitors described so far (leptomycin B and SINE compounds) interact with XPO1 through a covalent interaction with Cys528 located in the NES-binding cleft of XPO1. leptomycin B 38-50 exportin 1 Homo sapiens 169-173 34841476-6 2021 Interaction with CD47 was inhibited following alkylation of exportin-1 at Cys528 by its covalent inhibitor leptomycin B. leptomycin B 107-117 exportin 1 Homo sapiens 60-70 34841476-7 2021 Leptomycin B increased levels of m7G-modified RNAs, and their association with exportin-1 in EVs released from wild type but not CD47-deficient cells. leptomycin B 0-12 exportin 1 Homo sapiens 79-89 34271037-6 2021 Using the CRM1 inhibitor leptomycin B (LMB), we demonstrated that CRM1 inhibition promoted AcMNPV replication, as LMB treatment readily increased the virus titer, and even functionally surrogate Ac34 to rescue the infectivity of an ac34-knockout virus. leptomycin B 25-37 exportin 1 Homo sapiens 66-70 34271037-6 2021 Using the CRM1 inhibitor leptomycin B (LMB), we demonstrated that CRM1 inhibition promoted AcMNPV replication, as LMB treatment readily increased the virus titer, and even functionally surrogate Ac34 to rescue the infectivity of an ac34-knockout virus. leptomycin B 39-42 exportin 1 Homo sapiens 10-14 31416165-9 2019 Mutation of a putative nuclear export sequence (NES) in TRAIL-R2 or the inhibition of CRM-1 by Leptomycin-B resulted in the nuclear accumulation of TRAIL-R2. leptomycin B 95-107 exportin 1 Homo sapiens 86-91 33466582-7 2021 An inhibitor of the nuclear export protein CRM1, Leptomycin B, induced an increased expression of SOCS3 in the nucleus, but only a weak increase in nuclear JAK3. leptomycin B 49-61 exportin 1 Homo sapiens 43-47 33406424-3 2021 Nuclear accumulation is observed when leptomycin B is used to block the exportin, CRM1 protein. leptomycin B 38-50 exportin 1 Homo sapiens 82-86 31362890-7 2019 Blocking CRM1-dependent nuclear export with leptomycin B augmented PINK1 levels in the nucleus of MG132-treated cells but not in normal cells. leptomycin B 44-56 exportin 1 Homo sapiens 9-13 33658828-12 2021 And LMB, a Crm1 inhibitor, attenuated the effect of Hyp on Bach1 nuclear export and anti-oxidation. leptomycin B 4-7 exportin 1 Homo sapiens 11-15 32467326-5 2020 Interestingly, the exportin1/CRM1 inhibitor leptomycin-B abolishes the action of TPR peptide overexpression, although the drug cannot inhibit the GR nuclear export itself. leptomycin B 44-56 exportin 1 Homo sapiens 19-28 32467326-5 2020 Interestingly, the exportin1/CRM1 inhibitor leptomycin-B abolishes the action of TPR peptide overexpression, although the drug cannot inhibit the GR nuclear export itself. leptomycin B 44-56 exportin 1 Homo sapiens 29-33 31619134-3 2020 Here, we investigated the potential anti-cancer activities of leptomycin B, the most potent CRM1 antagonist, on cultured gastric carcinoma cells. leptomycin B 62-74 exportin 1 Homo sapiens 92-96 31676069-6 2020 Treatment of KHYG-1 cells with leptomycin B, a specific XPO1 inhibitor, increased the expression of GrB and IFN-gamma but did not potentiate lysis of specific target cells, suggesting that the cargo of XPO1 contributes to the expression of cytolytic genes but that this alone is insufficient to enhance cytolysis. leptomycin B 31-43 exportin 1 Homo sapiens 56-60 31676069-6 2020 Treatment of KHYG-1 cells with leptomycin B, a specific XPO1 inhibitor, increased the expression of GrB and IFN-gamma but did not potentiate lysis of specific target cells, suggesting that the cargo of XPO1 contributes to the expression of cytolytic genes but that this alone is insufficient to enhance cytolysis. leptomycin B 31-43 exportin 1 Homo sapiens 202-206 30826604-2 2019 The first inhibitor of nuclear transport, leptomycin B (LMB), was shown to be a specific inhibitor of the IMPbeta homologue Exportin 1 (EXP1) almost 20 years ago, but it has only been in the last five or so years that new inhibitors of nuclear export as well as import have been identified and characterised. leptomycin B 42-54 exportin 1 Homo sapiens 124-134 30826604-2 2019 The first inhibitor of nuclear transport, leptomycin B (LMB), was shown to be a specific inhibitor of the IMPbeta homologue Exportin 1 (EXP1) almost 20 years ago, but it has only been in the last five or so years that new inhibitors of nuclear export as well as import have been identified and characterised. leptomycin B 42-54 exportin 1 Homo sapiens 136-140 30826604-2 2019 The first inhibitor of nuclear transport, leptomycin B (LMB), was shown to be a specific inhibitor of the IMPbeta homologue Exportin 1 (EXP1) almost 20 years ago, but it has only been in the last five or so years that new inhibitors of nuclear export as well as import have been identified and characterised. leptomycin B 56-59 exportin 1 Homo sapiens 124-134 30826604-2 2019 The first inhibitor of nuclear transport, leptomycin B (LMB), was shown to be a specific inhibitor of the IMPbeta homologue Exportin 1 (EXP1) almost 20 years ago, but it has only been in the last five or so years that new inhibitors of nuclear export as well as import have been identified and characterised. leptomycin B 56-59 exportin 1 Homo sapiens 136-140 30953524-12 2019 Leptomycin B (LMB) treatment demonstrated that VP1 export from nucleus to cytoplasm occurred through a chromosome region maintenance 1 (CRM1)-dependent pathway. leptomycin B 0-12 exportin 1 Homo sapiens 136-140 30952896-5 2019 We demonstrate exemplarily that these NES motifs are sufficient to mediate export of GFP as model cargo and that this export can be inhibited by leptomycin B, an inhibitor of the Exportin 1/CRM1 pathway. leptomycin B 145-157 exportin 1 Homo sapiens 179-189 30952896-5 2019 We demonstrate exemplarily that these NES motifs are sufficient to mediate export of GFP as model cargo and that this export can be inhibited by leptomycin B, an inhibitor of the Exportin 1/CRM1 pathway. leptomycin B 145-157 exportin 1 Homo sapiens 190-194 30953524-12 2019 Leptomycin B (LMB) treatment demonstrated that VP1 export from nucleus to cytoplasm occurred through a chromosome region maintenance 1 (CRM1)-dependent pathway. leptomycin B 14-17 exportin 1 Homo sapiens 136-140 30941306-6 2019 EPS8 became nuclear localized when CRM1/Exportin 1-dependent nuclear export was inhibited by Leptomycin B, and a functional nuclear export signal could be identified within EPS8 using EGFP-tagging and site-directed mutagenesis. leptomycin B 93-105 exportin 1 Homo sapiens 35-39 30941306-6 2019 EPS8 became nuclear localized when CRM1/Exportin 1-dependent nuclear export was inhibited by Leptomycin B, and a functional nuclear export signal could be identified within EPS8 using EGFP-tagging and site-directed mutagenesis. leptomycin B 93-105 exportin 1 Homo sapiens 40-50 29930499-6 2018 Leptomycin B (LMB), an inhibitor of chromosomal maintenance 1 (CRM1), attenuated nucleocytoplasmic p27Kip1 export and astroglial proliferation, although it up-regulated ERK1/2 phosphorylation and GFAP expression. leptomycin B 0-12 exportin 1 Homo sapiens 63-67 30045709-14 2018 Treatment of CNDT2.5 and KRJ-I cells with the exportin-1 (XPO1/CRM1) inhibitor, leptomycin B, induced reduction in the cytoplasm and nuclear retention of TET2. leptomycin B 80-92 exportin 1 Homo sapiens 46-56 30045709-14 2018 Treatment of CNDT2.5 and KRJ-I cells with the exportin-1 (XPO1/CRM1) inhibitor, leptomycin B, induced reduction in the cytoplasm and nuclear retention of TET2. leptomycin B 80-92 exportin 1 Homo sapiens 58-62 30045709-14 2018 Treatment of CNDT2.5 and KRJ-I cells with the exportin-1 (XPO1/CRM1) inhibitor, leptomycin B, induced reduction in the cytoplasm and nuclear retention of TET2. leptomycin B 80-92 exportin 1 Homo sapiens 63-67 30660702-4 2019 CRM1 inhibitor Leptomycin B reduced drastically the presence of ERalpha and ERbeta in the extranuclear regions and increased in the nuclei, indicating the possible involvement of CRM1 on ERs nuclear-cytoplasmic shuttling. leptomycin B 15-27 exportin 1 Homo sapiens 0-4 30660702-4 2019 CRM1 inhibitor Leptomycin B reduced drastically the presence of ERalpha and ERbeta in the extranuclear regions and increased in the nuclei, indicating the possible involvement of CRM1 on ERs nuclear-cytoplasmic shuttling. leptomycin B 15-27 exportin 1 Homo sapiens 179-183 30576860-7 2019 In the presence of leptomycin B, P protein is retained in the nucleus, indicating that it contains a CRM1-dependent NES. leptomycin B 19-31 exportin 1 Homo sapiens 101-105 29748336-2 2018 CRM1/Xpo1 is the best-characterized exportin because specific inhibitors such as leptomycin B allow straightforward cargo validations in vivo. leptomycin B 81-93 exportin 1 Homo sapiens 0-4 29748336-2 2018 CRM1/Xpo1 is the best-characterized exportin because specific inhibitors such as leptomycin B allow straightforward cargo validations in vivo. leptomycin B 81-93 exportin 1 Homo sapiens 5-9 29930499-6 2018 Leptomycin B (LMB), an inhibitor of chromosomal maintenance 1 (CRM1), attenuated nucleocytoplasmic p27Kip1 export and astroglial proliferation, although it up-regulated ERK1/2 phosphorylation and GFAP expression. leptomycin B 14-17 exportin 1 Homo sapiens 63-67 30814875-8 2018 Crm1 is a powerful diagnostic biomarker because of its central role in cancerogenesis, and it has a high potential for the development of targeted Crm1 molecules or synthetic agents, such as LMB, as well as for the improvement of the clinical features in head and neck cancer. leptomycin B 191-194 exportin 1 Homo sapiens 0-4 30814875-8 2018 Crm1 is a powerful diagnostic biomarker because of its central role in cancerogenesis, and it has a high potential for the development of targeted Crm1 molecules or synthetic agents, such as LMB, as well as for the improvement of the clinical features in head and neck cancer. leptomycin B 191-194 exportin 1 Homo sapiens 147-151 29228114-6 2018 Inhibition of XPO1 by a specific inhibitor, leptomycin B, delayed the GV breakdown (GVBD), whereas the overexpression of XPO1 by mRNA injection accelerated the GVBD. leptomycin B 44-56 exportin 1 Homo sapiens 14-18 28412356-0 2017 Leptomycin B alters the subcellular distribution of CRM1 (Exportin 1). leptomycin B 0-12 exportin 1 Homo sapiens 52-56 29208528-3 2018 Leptomycin B treatment of myoblasts increased nuclear HP1gamma, suggesting that its nuclear export is Crm-1-dependent. leptomycin B 0-12 exportin 1 Homo sapiens 102-107 28702009-8 2017 Leptomycin B (LMB) is the prototypical inhibitor of CRM1 potent against various cancer cell lines overexpressing CRM1 and in limiting viral infections at nanomolar concentrations in vitro. leptomycin B 0-12 exportin 1 Homo sapiens 52-56 28702009-8 2017 Leptomycin B (LMB) is the prototypical inhibitor of CRM1 potent against various cancer cell lines overexpressing CRM1 and in limiting viral infections at nanomolar concentrations in vitro. leptomycin B 0-12 exportin 1 Homo sapiens 113-117 28702009-8 2017 Leptomycin B (LMB) is the prototypical inhibitor of CRM1 potent against various cancer cell lines overexpressing CRM1 and in limiting viral infections at nanomolar concentrations in vitro. leptomycin B 14-17 exportin 1 Homo sapiens 52-56 28702009-8 2017 Leptomycin B (LMB) is the prototypical inhibitor of CRM1 potent against various cancer cell lines overexpressing CRM1 and in limiting viral infections at nanomolar concentrations in vitro. leptomycin B 14-17 exportin 1 Homo sapiens 113-117 28942004-3 2017 The aim of this study was to explore the potential utility of chromosome region maintenance 1 (CRM1) inhibitor leptomycin B (LMB) in combination with gefitinib to overcome primary and acquired gefitinib resistance in NSCLC cells. leptomycin B 111-123 exportin 1 Homo sapiens 95-99 28942004-3 2017 The aim of this study was to explore the potential utility of chromosome region maintenance 1 (CRM1) inhibitor leptomycin B (LMB) in combination with gefitinib to overcome primary and acquired gefitinib resistance in NSCLC cells. leptomycin B 125-128 exportin 1 Homo sapiens 95-99 28412356-0 2017 Leptomycin B alters the subcellular distribution of CRM1 (Exportin 1). leptomycin B 0-12 exportin 1 Homo sapiens 58-68 28412356-2 2017 Leptomycin B (LMB), the first specific inhibitor of CRM1 identified, binds covalently to cysteine 528 in the nuclear export signal binding region of CRM1 leading to the inhibition of protein nuclear export. leptomycin B 0-12 exportin 1 Homo sapiens 52-56 28412356-2 2017 Leptomycin B (LMB), the first specific inhibitor of CRM1 identified, binds covalently to cysteine 528 in the nuclear export signal binding region of CRM1 leading to the inhibition of protein nuclear export. leptomycin B 0-12 exportin 1 Homo sapiens 149-153 28412356-2 2017 Leptomycin B (LMB), the first specific inhibitor of CRM1 identified, binds covalently to cysteine 528 in the nuclear export signal binding region of CRM1 leading to the inhibition of protein nuclear export. leptomycin B 14-17 exportin 1 Homo sapiens 52-56 28412356-2 2017 Leptomycin B (LMB), the first specific inhibitor of CRM1 identified, binds covalently to cysteine 528 in the nuclear export signal binding region of CRM1 leading to the inhibition of protein nuclear export. leptomycin B 14-17 exportin 1 Homo sapiens 149-153 28412356-3 2017 Although the biochemical mechanisms of action of CRM1 inhibitors such as LMB are well studied, the subcellular effects of inhibition on CRM1 are unknown. leptomycin B 73-76 exportin 1 Homo sapiens 49-53 28412356-7 2017 GFP-tagged CRM1 protein microinjected into the cytoplasm of A549 cells distributed throughout the cell in untreated cells remained primarily cytoplasmic in LMB-treated cells. leptomycin B 156-159 exportin 1 Homo sapiens 11-15 28412356-8 2017 Upon nuclear microinjection, GFP-CRM1 translocated to and accumulated in the cytoplasm of LMB-treated cells. leptomycin B 90-93 exportin 1 Homo sapiens 33-37 26429201-4 2016 In this study, endogenous OGFr, as well as exogenously expressed OGFr-EGFP, demonstrated significant nuclear accumulation in response to leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export, suggesting that OGFr is exported in a CRM1-dependent manner. leptomycin B 137-149 exportin 1 Homo sapiens 173-177 27902702-3 2016 One major limitation of Leptomycin B is its irreversible binding to CRM1; which SINE compounds alleviate because they are slowly reversible. leptomycin B 24-36 exportin 1 Homo sapiens 68-72 27613868-9 2016 SET-Nup214 nuclear bodies are highly mobile structures, which are in equilibrium with the nucleoplasm in interphase and disassemble during mitosis or upon treatment of cells with the CRM1-inhibitor leptomycin B. leptomycin B 198-210 exportin 1 Homo sapiens 183-187 27113410-8 2016 However, clinical development of the exportin-1 inhibitor leptomycin B was stopped due to adverse events, including vomiting, anorexia, and dehydration. leptomycin B 58-70 exportin 1 Homo sapiens 37-47 26449823-8 2016 Furthermore, we detected that leptomycin B, the inhibitor of CRM1 protein, could recover nuclear translocation of endogenous Smads complex in HL7702 with HBX expression, indicating that HBX antagonized Smads nucleus translocation, at least partially, on CRM1-dependent manner. leptomycin B 30-42 exportin 1 Homo sapiens 61-65 26449823-8 2016 Furthermore, we detected that leptomycin B, the inhibitor of CRM1 protein, could recover nuclear translocation of endogenous Smads complex in HL7702 with HBX expression, indicating that HBX antagonized Smads nucleus translocation, at least partially, on CRM1-dependent manner. leptomycin B 30-42 exportin 1 Homo sapiens 254-258 26429201-4 2016 In this study, endogenous OGFr, as well as exogenously expressed OGFr-EGFP, demonstrated significant nuclear accumulation in response to leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export, suggesting that OGFr is exported in a CRM1-dependent manner. leptomycin B 137-149 exportin 1 Homo sapiens 242-246 26429201-4 2016 In this study, endogenous OGFr, as well as exogenously expressed OGFr-EGFP, demonstrated significant nuclear accumulation in response to leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export, suggesting that OGFr is exported in a CRM1-dependent manner. leptomycin B 151-154 exportin 1 Homo sapiens 173-177 26429201-4 2016 In this study, endogenous OGFr, as well as exogenously expressed OGFr-EGFP, demonstrated significant nuclear accumulation in response to leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export, suggesting that OGFr is exported in a CRM1-dependent manner. leptomycin B 151-154 exportin 1 Homo sapiens 242-246 26402707-7 2015 The first compound found to inhibit CRM1-dependent nuclear export was the natural drug Leptomycin B (LMB), which blocks export by competitively interacting with a highly conserved cleft on CRM1 required for nuclear export signal recognition. leptomycin B 87-99 exportin 1 Homo sapiens 36-40 26654943-4 2016 Biotinylated leptomycin B (b-LMB) was utilized as a tool compound to measure SINE-free XPO1. leptomycin B 13-25 exportin 1 Homo sapiens 87-91 26506250-4 2015 Here we show that inhibition of CRM1-mediated nuclear export using RNAi-knockdown of CRM1 and inactivation of CRM1 by leptomycin B (LMB) results in nuclear accumulation of RanGAP1. leptomycin B 132-135 exportin 1 Homo sapiens 32-36 26506250-5 2015 LMB treatment induced a more robust redistribution of RanGAP1 from the cytoplasm to the nucleoplasm compared to CRM1 RNAi and also uniquely triggered a decrease or loss of RanGAP1 localization at the NPC, suggesting that LMB treatment is more effective in inhibiting CRM1-mediated nuclear export of RanGAP1. leptomycin B 0-3 exportin 1 Homo sapiens 267-271 26402707-7 2015 The first compound found to inhibit CRM1-dependent nuclear export was the natural drug Leptomycin B (LMB), which blocks export by competitively interacting with a highly conserved cleft on CRM1 required for nuclear export signal recognition. leptomycin B 87-99 exportin 1 Homo sapiens 189-193 26402707-7 2015 The first compound found to inhibit CRM1-dependent nuclear export was the natural drug Leptomycin B (LMB), which blocks export by competitively interacting with a highly conserved cleft on CRM1 required for nuclear export signal recognition. leptomycin B 101-104 exportin 1 Homo sapiens 36-40 26402707-7 2015 The first compound found to inhibit CRM1-dependent nuclear export was the natural drug Leptomycin B (LMB), which blocks export by competitively interacting with a highly conserved cleft on CRM1 required for nuclear export signal recognition. leptomycin B 101-104 exportin 1 Homo sapiens 189-193 25894517-7 2015 Interestingly, leptomycin B, an inhibitor of CRM1-mediated export, or siRNA mediated depletion of CRM1, significantly impaired the inter-cellular transport of Ran, suggesting a function for CRM1 in this process. leptomycin B 15-27 exportin 1 Homo sapiens 45-49 26048327-6 2015 Therapeutic targeting of CRM1 has emerged as a novel cancer treatment strategy, starting with a clinical trial with leptomycin B, the original specific inhibitor of CRM1, followed by development of several next-generation small molecules. leptomycin B 116-128 exportin 1 Homo sapiens 25-29 26048327-6 2015 Therapeutic targeting of CRM1 has emerged as a novel cancer treatment strategy, starting with a clinical trial with leptomycin B, the original specific inhibitor of CRM1, followed by development of several next-generation small molecules. leptomycin B 116-128 exportin 1 Homo sapiens 165-169 25911113-3 2015 However, two lines of evidence suggest TR also follows another pathway: export is only partially blocked by leptomycin B (LMB), a CRM1-specific inhibitor; and we identified nuclear export signals in TR that are LMB-resistant. leptomycin B 108-120 exportin 1 Homo sapiens 130-134 25911113-3 2015 However, two lines of evidence suggest TR also follows another pathway: export is only partially blocked by leptomycin B (LMB), a CRM1-specific inhibitor; and we identified nuclear export signals in TR that are LMB-resistant. leptomycin B 122-125 exportin 1 Homo sapiens 130-134 25629636-6 2015 Indeed, we showed that the cytotoxic effects of cisplatin on A549 cells with CRM1 down-regulated by short hairpin siRNA were significantly increased, compared with A549 cells, and the cytotoxic effects of cisplatin became further enhanced when the drug was used in combination with leptomycin B, a CRM1 inhibitor, in both in vitro and in vivo models. leptomycin B 282-294 exportin 1 Homo sapiens 77-81 25793203-9 2015 Inhibition of nuclear export by leptomycin B suggests a model in which exportin-1 acts as a decoy, in hypoxic cells, preventing carbonic anhydrase IX association with 45 S rDNA gene promoters. leptomycin B 32-44 exportin 1 Homo sapiens 71-81 25886724-4 2015 RESULTS: In this work, a dynamic export process of Mps1 from the nucleus to cytoplasm in interphase was revealed- a process blocked by the Crm1 inhibitor, Leptomycin B, suggesting that export of Mps1 is Crm1 dependent. leptomycin B 155-167 exportin 1 Homo sapiens 139-143 25886724-4 2015 RESULTS: In this work, a dynamic export process of Mps1 from the nucleus to cytoplasm in interphase was revealed- a process blocked by the Crm1 inhibitor, Leptomycin B, suggesting that export of Mps1 is Crm1 dependent. leptomycin B 155-167 exportin 1 Homo sapiens 203-207 25449906-8 2015 A specific inhibitor of exportin 1 (leptomycin B) also caused NIs. leptomycin B 36-48 exportin 1 Homo sapiens 24-34 26275159-5 2015 Upon blockage of the CRM1-dependent nuclear export by leptomycin B (LMB), CNoBs disappear while p62/SQSTM1-containing fibrillar nuclear bodies are induced. leptomycin B 54-66 exportin 1 Homo sapiens 21-25 26275159-5 2015 Upon blockage of the CRM1-dependent nuclear export by leptomycin B (LMB), CNoBs disappear while p62/SQSTM1-containing fibrillar nuclear bodies are induced. leptomycin B 68-71 exportin 1 Homo sapiens 21-25 24898882-8 2014 Normal oesophageal epithelial cells, however, were much less sensitive to Crm1 inhibition with siRNA and LMB. leptomycin B 105-108 exportin 1 Homo sapiens 74-78 25476752-9 2014 The best understood XPO1 inhibitors are the small molecule nuclear export inhibitors (NEIs; Leptomycin B and derivatives, ratjadones, PKF050-638, valtrate, ACA, CBS9106, selinexor/KPT-330, and verdinexor/KPT-335). leptomycin B 92-104 exportin 1 Homo sapiens 20-24 24631834-9 2014 Clinical trials using the classic CRM1 inhibitor leptomycin B proved too toxic for patients; however, a new generation of less toxic small molecule inhibitors is being used in clinical trials in patients with both hematological malignancies and solid tumors. leptomycin B 49-61 exportin 1 Homo sapiens 34-38 24631835-4 2014 Numerous atomic resolution CRM1 structures are now available, explaining how the exporter recognizes nuclear export signals in its cargos, how RanGTP and cargo bind with positive cooperativity, how RanBP1 causes release of export cargos in the cytoplasm and how diverse inhibitors such as Leptomycin B and the new KPT-SINE compounds block nuclear export. leptomycin B 289-301 exportin 1 Homo sapiens 27-31 24161512-5 2013 Mifepristone and ivermectin, inhibitors of importin alpha/beta-mediated import, were able to reduce nuclear-associated capsid, while leptomycin B, a potent CRM1 inhibitor, confined capsid to the nucleus. leptomycin B 133-145 exportin 1 Homo sapiens 156-160 23530499-5 2014 However, natural product, irreversible XPO1 antagonists such as leptomycin B (LMB) have proven toxic in both preclinical models and in the clinic. leptomycin B 64-76 exportin 1 Homo sapiens 39-43 23530499-5 2014 However, natural product, irreversible XPO1 antagonists such as leptomycin B (LMB) have proven toxic in both preclinical models and in the clinic. leptomycin B 78-81 exportin 1 Homo sapiens 39-43 24099702-4 2014 Leptomycin B inhibited the activity of AR(50-250), suggesting that AR(50-250) export is mediated through exportin 1, either directly or indirectly. leptomycin B 0-12 exportin 1 Homo sapiens 105-115 25072814-5 2014 We also found that export of NOA1 from the nucleus requires a leptomycin-B sensitive, Crm1-dependent nuclear export signal (NES). leptomycin B 62-74 exportin 1 Homo sapiens 86-90 24857738-5 2014 Applying this approach to cells treated with or without the selective CRM1 inhibitor leptomycin B, we identified substrates of CRM1, the major nuclear export receptor. leptomycin B 85-97 exportin 1 Homo sapiens 70-74 24857738-5 2014 Applying this approach to cells treated with or without the selective CRM1 inhibitor leptomycin B, we identified substrates of CRM1, the major nuclear export receptor. leptomycin B 85-97 exportin 1 Homo sapiens 127-131 23950892-3 2013 This process was inhibited by leptomycine B (LMB), a specific inhibitor of nuclear export receptor chromosomal region maintenance 1 (CRM1). leptomycin B 45-48 exportin 1 Homo sapiens 99-131 23882114-7 2013 Furthermore, deletion of the N-terminal helicase domain results in partial accumulation of Dicer in the nucleus upon leptomycin B treatment, indicating that CRM1 contributes to nuclear export of Dicer. leptomycin B 117-129 exportin 1 Homo sapiens 157-161 23850870-7 2013 Trapping of S7ORF2 in the nucleus was obtained by treatment with leptomycin B, an inhibitor of CRM1-mediated nuclear export, indicating that S7ORF2 use CRM1 for the nuclear exit. leptomycin B 65-77 exportin 1 Homo sapiens 95-99 23850870-7 2013 Trapping of S7ORF2 in the nucleus was obtained by treatment with leptomycin B, an inhibitor of CRM1-mediated nuclear export, indicating that S7ORF2 use CRM1 for the nuclear exit. leptomycin B 65-77 exportin 1 Homo sapiens 152-156 23940654-7 2013 The partition of miRNAs between the nucleolus and the cytoplasm is affected by Leptomycin B, suggesting a role of Exportin-1 in the intracellular shuttling of miRNAs. leptomycin B 79-91 exportin 1 Homo sapiens 114-124 23950892-3 2013 This process was inhibited by leptomycine B (LMB), a specific inhibitor of nuclear export receptor chromosomal region maintenance 1 (CRM1). leptomycin B 45-48 exportin 1 Homo sapiens 133-137 23740995-1 2013 Leptomycin B (LMB) is a highly specific inhibitor of CRM1, a cellular karyopherin-beta that transports nuclear export signal-containing proteins from the nucleus to the cytoplasm. leptomycin B 0-12 exportin 1 Homo sapiens 53-57 23740995-1 2013 Leptomycin B (LMB) is a highly specific inhibitor of CRM1, a cellular karyopherin-beta that transports nuclear export signal-containing proteins from the nucleus to the cytoplasm. leptomycin B 14-17 exportin 1 Homo sapiens 53-57 23471078-5 2013 Treatment of DLD-1 cells with the CRM1 inhibitor leptomycin B (LMB) or anti-CRM1 siRNAs reduced cytokine-induced iNOS expression. leptomycin B 49-61 exportin 1 Homo sapiens 34-38 23523789-5 2013 It was also blocked by co-treatment with the CRM1-specific nuclear export inhibitor leptomycin B (LMB), but not with the inhibitors for mitogen-activated protein kinases (MAPKs). leptomycin B 84-96 exportin 1 Homo sapiens 45-49 23523789-5 2013 It was also blocked by co-treatment with the CRM1-specific nuclear export inhibitor leptomycin B (LMB), but not with the inhibitors for mitogen-activated protein kinases (MAPKs). leptomycin B 98-101 exportin 1 Homo sapiens 45-49 23782956-8 2013 While inhibition of CRM1 by leptomycin B inhibited processing of 28S ribosomal (r) RNA, depletion of NMD3 did not, suggesting that their effects on 28S rRNA processing are distinct. leptomycin B 28-40 exportin 1 Homo sapiens 20-24 23667685-7 2013 We present data demonstrating that URI nuclear/cytoplasmic shuttling is affected by compounds that stall pol II on the DNA (alpha-amanitin and actinomycin-D) and by leptomycin B, an inhibitor of the CRM1 exportin that mediates the nuclear export of pol II subunits. leptomycin B 165-177 exportin 1 Homo sapiens 199-203 23471078-5 2013 Treatment of DLD-1 cells with the CRM1 inhibitor leptomycin B (LMB) or anti-CRM1 siRNAs reduced cytokine-induced iNOS expression. leptomycin B 63-66 exportin 1 Homo sapiens 34-38 23369348-9 2013 Finally, upon inhibition of CRM1-dependent nuclear export with Leptomycin B (LMB), wild type rhTRIM5alpha localized to SUMO-1 bodies in the nucleus, while the SIM1 and SIM2 mutants did not localize to SUMO-1. leptomycin B 63-75 exportin 1 Homo sapiens 28-32 23369348-9 2013 Finally, upon inhibition of CRM1-dependent nuclear export with Leptomycin B (LMB), wild type rhTRIM5alpha localized to SUMO-1 bodies in the nucleus, while the SIM1 and SIM2 mutants did not localize to SUMO-1. leptomycin B 77-80 exportin 1 Homo sapiens 28-32 22846997-4 2012 We showed here that leptomycin B, an inhibitor of the chromosome region maintenance 1 (CRM1) exportin, inhibited PCNA relocalization during granulocytic differentiation of HL-60 and NB4 promyelocytic cell lines and of human CD34(+) primary cells. leptomycin B 20-32 exportin 1 Homo sapiens 87-91 23297231-0 2013 Nuclear export inhibition through covalent conjugation and hydrolysis of Leptomycin B by CRM1. leptomycin B 73-85 exportin 1 Homo sapiens 89-93 23297231-1 2013 The polyketide natural product Leptomycin B inhibits nuclear export mediated by the karyopherin protein chromosomal region maintenance 1 (CRM1). leptomycin B 31-43 exportin 1 Homo sapiens 104-136 23297231-1 2013 The polyketide natural product Leptomycin B inhibits nuclear export mediated by the karyopherin protein chromosomal region maintenance 1 (CRM1). leptomycin B 31-43 exportin 1 Homo sapiens 138-142 23297231-2 2013 Here, we present 1.8- to 2.0-A-resolution crystal structures of CRM1 bound to Leptomycin B and related inhibitors Anguinomycin A and Ratjadone A. leptomycin B 78-90 exportin 1 Homo sapiens 64-68 22796641-3 2012 A XAB1/Gpn1-EYFP fluorescent protein, like endogenous XAB1/Gpn1, localized to the cytoplasm but it rapidly accumulated in the cell nucleus in the presence of leptomycin B, a chemical inhibitor of the nuclear transport receptor Crm1. leptomycin B 158-170 exportin 1 Homo sapiens 227-231 22695114-6 2012 The nuclear export of GATA-6 was inhibited by leptomycin B, suggesting that CRM1-mediated export could be activated by anisomycin. leptomycin B 46-58 exportin 1 Homo sapiens 76-80 22895175-7 2012 The cytoplasmic LIN54 mutant accumulated in the nucleus after leptomycin B treatment, suggesting CRM1-mediated nuclear export of LIN54. leptomycin B 62-74 exportin 1 Homo sapiens 97-101 22623727-3 2012 Here, we characterize the active nuclear export of cofilin through a leptomycin-B-sensitive, CRM1-dependent, nuclear export signal (NES). leptomycin B 69-81 exportin 1 Homo sapiens 93-97 23885315-2 2012 The nuclear accumulation of IkappaBalpha can be induced by post-induction repression in stimulated cells, inhibition of the CRM1-dependent nuclear IkappaBalpha export by leptomycin B, and by the inhibition of the 26S proteasome. leptomycin B 170-182 exportin 1 Homo sapiens 124-128 22414299-8 2012 Like other proteins containing a leucine-rich export signal, nuclear export of the UL94 was affected by leptomycin B, indicating that it is exported via the Crm1-dependent pathway. leptomycin B 104-116 exportin 1 Homo sapiens 157-161 21986938-8 2012 DLC-induced Nur77 protein was mainly found in the cytoplasm, and inhibition of Nur77 nuclear export by the CRM1-dependent nuclear export inhibitor leptomycin B or Jun N-terminal kinase inhibitor prevented the effect of DLC on inducing beta-catenin degradation. leptomycin B 147-159 exportin 1 Homo sapiens 107-111 22318152-9 2012 In addition, Rex-mediated export of tax/rex RNA was CRM1 dependent and inhibited by leptomycin B treatment. leptomycin B 84-96 exportin 1 Homo sapiens 52-56 22516749-6 2012 Furthermore, CRM1-specific nuclear export inhibitor LMB blocked the hNANOG potent NES-mediated export, suggesting that the leucine-rich motif may function in CRM1-mediated nuclear export of hNANOG. leptomycin B 52-55 exportin 1 Homo sapiens 13-17 22516749-6 2012 Furthermore, CRM1-specific nuclear export inhibitor LMB blocked the hNANOG potent NES-mediated export, suggesting that the leucine-rich motif may function in CRM1-mediated nuclear export of hNANOG. leptomycin B 52-55 exportin 1 Homo sapiens 158-162 22155563-5 2012 Nuclear accumulation of NLS/nuclear export signal-containing (shuttling) proteins was also enhanced in transformed cell types, experiments using the nuclear export inhibitor leptomycin B demonstrating that efficient Exp-1-mediated nuclear export was not impaired in transformed compared with nontransformed cells. leptomycin B 174-186 exportin 1 Homo sapiens 216-221 22558287-12 2012 Export of BAT3 from the nucleus to the cytosol is inhibited by treatment with leptomycin B, indicating that the Crm1 pathway is involved. leptomycin B 78-90 exportin 1 Homo sapiens 112-116 21888622-6 2012 We tested the effect of the CRM1 inhibitor LMB (leptomycin B) on the localization of YFP (yellow fluorescent protein)- or GFP (green fluorescent protein)-tagged versions of six NES-bearing deubiquitinases [USP (ubiquitin-specific peptidase) 1, USP3, USP7, USP21, CYLD (cylindromatosis) and OTUD7B (OTU-domain-containing 7B)]. leptomycin B 43-46 exportin 1 Homo sapiens 28-32 21888622-6 2012 We tested the effect of the CRM1 inhibitor LMB (leptomycin B) on the localization of YFP (yellow fluorescent protein)- or GFP (green fluorescent protein)-tagged versions of six NES-bearing deubiquitinases [USP (ubiquitin-specific peptidase) 1, USP3, USP7, USP21, CYLD (cylindromatosis) and OTUD7B (OTU-domain-containing 7B)]. leptomycin B 48-60 exportin 1 Homo sapiens 28-32 22113272-3 2012 We have previously shown that in human leukocytes, transcription of NFkappaB-dependent genes is inhibited by the nuclear translocation and accumulation of IkappaBalpha, which can be induced by an inhibitor of CRM1-dependent nuclear export, leptomycin B (LMB). leptomycin B 254-257 exportin 1 Homo sapiens 209-213 22384264-8 2012 Treatment with leptomycin B (LMB), an inhibitor of the nuclear export receptor CRM1, resulted in a significant increase in the percentage of cells with nuclear MIER1-3Aalpha, from 4% to 53%, demonstrating that cytoplasmic localization of this isoform was due to CRM1-dependent nuclear export. leptomycin B 15-27 exportin 1 Homo sapiens 79-83 22384264-8 2012 Treatment with leptomycin B (LMB), an inhibitor of the nuclear export receptor CRM1, resulted in a significant increase in the percentage of cells with nuclear MIER1-3Aalpha, from 4% to 53%, demonstrating that cytoplasmic localization of this isoform was due to CRM1-dependent nuclear export. leptomycin B 15-27 exportin 1 Homo sapiens 262-266 22384264-8 2012 Treatment with leptomycin B (LMB), an inhibitor of the nuclear export receptor CRM1, resulted in a significant increase in the percentage of cells with nuclear MIER1-3Aalpha, from 4% to 53%, demonstrating that cytoplasmic localization of this isoform was due to CRM1-dependent nuclear export. leptomycin B 29-32 exportin 1 Homo sapiens 79-83 22384264-8 2012 Treatment with leptomycin B (LMB), an inhibitor of the nuclear export receptor CRM1, resulted in a significant increase in the percentage of cells with nuclear MIER1-3Aalpha, from 4% to 53%, demonstrating that cytoplasmic localization of this isoform was due to CRM1-dependent nuclear export. leptomycin B 29-32 exportin 1 Homo sapiens 262-266 23251541-5 2012 Inhibition of the Rev:CRM1 interaction by leptomycin B or a non-interacting RevM10 mutant completely blocked redistribution of Rev by Nullbasic. leptomycin B 42-54 exportin 1 Homo sapiens 22-26 21953454-6 2011 nCLU accumulation was regulated by CRM1 because binding between CRM1 and nCLU proteins was significantly diminished by leptomycin B (LMB), and nuclear levels of nCLU protein were significantly enhanced by LMB and IR co-treatment. leptomycin B 119-131 exportin 1 Homo sapiens 35-39 21953454-6 2011 nCLU accumulation was regulated by CRM1 because binding between CRM1 and nCLU proteins was significantly diminished by leptomycin B (LMB), and nuclear levels of nCLU protein were significantly enhanced by LMB and IR co-treatment. leptomycin B 119-131 exportin 1 Homo sapiens 64-68 21953454-6 2011 nCLU accumulation was regulated by CRM1 because binding between CRM1 and nCLU proteins was significantly diminished by leptomycin B (LMB), and nuclear levels of nCLU protein were significantly enhanced by LMB and IR co-treatment. leptomycin B 133-136 exportin 1 Homo sapiens 35-39 21953454-6 2011 nCLU accumulation was regulated by CRM1 because binding between CRM1 and nCLU proteins was significantly diminished by leptomycin B (LMB), and nuclear levels of nCLU protein were significantly enhanced by LMB and IR co-treatment. leptomycin B 133-136 exportin 1 Homo sapiens 64-68 21561424-3 2011 The structural basis for the inhibition of nuclear export by leptomycin (LMB) based on disruption of the protein/protein interaction between CRM1 and cargo proteins is discussed. leptomycin B 61-71 exportin 1 Homo sapiens 141-145 21777610-4 2011 Upon LMB treatment we found that FTS was located in the nucleus and it suggests that direct role of nuclear export signal (NES) is required for the binding to CRM1 and facilitates nuclear export. leptomycin B 5-8 exportin 1 Homo sapiens 159-163 21561424-3 2011 The structural basis for the inhibition of nuclear export by leptomycin (LMB) based on disruption of the protein/protein interaction between CRM1 and cargo proteins is discussed. leptomycin B 73-76 exportin 1 Homo sapiens 141-145 20677820-2 2010 Over the past decade, small molecules that inhibit the nuclear export receptor CRM1 have been identified, most notably leptomycin B. leptomycin B 119-131 exportin 1 Homo sapiens 79-83 21756326-4 2011 Furthermore, we evaluated EGFR expression in ten ovarian cancer cell lines and incubated cancer cells with Leptomycin B, a CRM1 specific inhibitor. leptomycin B 107-119 exportin 1 Homo sapiens 123-127 20946012-9 2010 Inhibition of Exp1 with leptomycin B somewhat slowed down the clearance of ODNs from the nucleus; however, within 6 hours most of the ODN were still being cleared form the nucleus. leptomycin B 24-36 exportin 1 Homo sapiens 14-18 20869252-3 2010 Leptomycin B, a small molecule inhibitor of the exportin CRM1, has proved to be an invaluable tool for cell biologists, but up to now no small molecule inhibitors of nuclear import have been described. leptomycin B 0-12 exportin 1 Homo sapiens 57-61 20457605-3 2010 Like leptomycin B (LMB), an established fungal CRM1-inhibitor, 15d-PGJ(2) reacts with a conserved cysteine residue in the CRM1 sequence. leptomycin B 5-17 exportin 1 Homo sapiens 47-51 20857263-5 2010 Leptomycin B (LMB) inhibition of the CRM1 pathway decreased the export of core element RNA, implying that the principal site of CRM1 action for exporting IFN-alpha1 mRNA resides within the core element. leptomycin B 0-12 exportin 1 Homo sapiens 37-41 20857263-5 2010 Leptomycin B (LMB) inhibition of the CRM1 pathway decreased the export of core element RNA, implying that the principal site of CRM1 action for exporting IFN-alpha1 mRNA resides within the core element. leptomycin B 0-12 exportin 1 Homo sapiens 128-132 20857263-5 2010 Leptomycin B (LMB) inhibition of the CRM1 pathway decreased the export of core element RNA, implying that the principal site of CRM1 action for exporting IFN-alpha1 mRNA resides within the core element. leptomycin B 14-17 exportin 1 Homo sapiens 37-41 20857263-5 2010 Leptomycin B (LMB) inhibition of the CRM1 pathway decreased the export of core element RNA, implying that the principal site of CRM1 action for exporting IFN-alpha1 mRNA resides within the core element. leptomycin B 14-17 exportin 1 Homo sapiens 128-132 20567258-2 2010 Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B (LMB), a specific inhibitor of Crm1, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. leptomycin B 92-104 exportin 1 Homo sapiens 136-140 20567258-2 2010 Although pericentrin was shown to localize in the cytoplasm and reported to be sensitive to leptomycin B (LMB), a specific inhibitor of Crm1, the regions within pericentrin that serve as signals for transporting in and out of the nucleus have not yet been identified. leptomycin B 106-109 exportin 1 Homo sapiens 136-140 20489206-8 2010 The nuclear export of p42 AUF1 was driven by c-Raf/MEK1/2/ERK1/2 signaling and sensitive to leptomycin B treatment, suggesting a CRM1-dependent mechanism. leptomycin B 92-104 exportin 1 Homo sapiens 129-133 20457605-3 2010 Like leptomycin B (LMB), an established fungal CRM1-inhibitor, 15d-PGJ(2) reacts with a conserved cysteine residue in the CRM1 sequence. leptomycin B 19-22 exportin 1 Homo sapiens 122-126 20457605-5 2010 Cells that are transfected with mutant CRM1 (C528S) are resistant to the inhibitory effects of LMB and 15d-PGJ(2), demonstrating that the same single amino acid is targeted by the two compounds. leptomycin B 95-98 exportin 1 Homo sapiens 39-43 20457605-3 2010 Like leptomycin B (LMB), an established fungal CRM1-inhibitor, 15d-PGJ(2) reacts with a conserved cysteine residue in the CRM1 sequence. leptomycin B 5-17 exportin 1 Homo sapiens 122-126 20457605-3 2010 Like leptomycin B (LMB), an established fungal CRM1-inhibitor, 15d-PGJ(2) reacts with a conserved cysteine residue in the CRM1 sequence. leptomycin B 19-22 exportin 1 Homo sapiens 47-51 20375145-3 2010 Nuclear, but not cytoplasmic, dots of EGFP-tagged Nup98 disappeared rapidly after cell treatment with leptomycin B, a specific inhibitor of the nuclear export receptor, Crm1. leptomycin B 102-114 exportin 1 Homo sapiens 169-173 20378546-5 2010 The inactivation of CRM1 by leptomycin B or by siRNA knockdown caused nuclear accumulation of ectopic and endogenous B56alpha. leptomycin B 28-40 exportin 1 Homo sapiens 20-24 20026644-7 2010 The CRM1 inhibitor leptomycin B abolished cytosolic PPARgamma localization, suggesting that this is a CRM1-dependent export process. leptomycin B 19-31 exportin 1 Homo sapiens 4-8 19545931-3 2010 Here we show that Leptomycin B (LMB), a highly specific inhibitor of the nuclear export receptor CRM1, abrogates the ability of DC to stimulate T cells in an allogeneic mixed lymphocyte reaction. leptomycin B 18-30 exportin 1 Homo sapiens 97-101 19545931-3 2010 Here we show that Leptomycin B (LMB), a highly specific inhibitor of the nuclear export receptor CRM1, abrogates the ability of DC to stimulate T cells in an allogeneic mixed lymphocyte reaction. leptomycin B 32-35 exportin 1 Homo sapiens 97-101 20346347-4 2010 HXKII is extruded from the nucleus, at least in part, by the activity of the exportin 1/CrmA system, as demonstrated by increased nuclear expression and decreased cytoplasmic expression after incubation with leptomycin B, a bacterially-derived exportin inhibitor. leptomycin B 208-220 exportin 1 Homo sapiens 77-87 20127713-7 2010 The nuclear export of AR following GSK-3beta inhibition could be blocked by leptomycin B suggesting a CRM1-dependent export mechanism. leptomycin B 76-88 exportin 1 Homo sapiens 102-106 20064523-4 2010 Using heterokaryon assay, we have demonstrated that RASSF5 shuttles between the nucleus and the cytoplasm, and its export from the nucleus is sensitive to leptomycin B, suggesting that RASSF5 is exported from the nucleus by a CRM-1-dependent export pathway. leptomycin B 155-167 exportin 1 Homo sapiens 226-231 20026644-7 2010 The CRM1 inhibitor leptomycin B abolished cytosolic PPARgamma localization, suggesting that this is a CRM1-dependent export process. leptomycin B 19-31 exportin 1 Homo sapiens 102-106 20016063-5 2010 cFLIP-L also has a nuclear export signal (NES) near the NLS, and leptomycin B, an inhibitor of CRM1-dependent nuclear export, increases the nuclear accumulation of cFLIP-L, suggesting that it shuttles between the nucleus and cytoplasm. leptomycin B 65-77 exportin 1 Homo sapiens 95-99 19602195-5 2009 AP21967-induced nuclear export was specifically inhibited by the Crm1-binding molecule leptomycin B. leptomycin B 87-99 exportin 1 Homo sapiens 65-69 19858290-12 2010 Moreover, treatment with the Crm1 inhibitor leptomycin B or independent mutation of the potential NES (NESm) abolished Wee1 nuclear export. leptomycin B 44-56 exportin 1 Homo sapiens 29-33 19955415-3 2009 Inhibition of CRM1 by Leptomycin B results in nuclear accumulation of miRNA guide sequences. leptomycin B 22-34 exportin 1 Homo sapiens 14-18 19422798-8 2009 Since pericentrin was sensitive to Crm1 specific inhibitor leptomycin B, we propose that the centrosomal Crm1 might interact with pericentrin and regulate the localization and function of pericentrin at centrosomes. leptomycin B 59-71 exportin 1 Homo sapiens 35-39 19422798-8 2009 Since pericentrin was sensitive to Crm1 specific inhibitor leptomycin B, we propose that the centrosomal Crm1 might interact with pericentrin and regulate the localization and function of pericentrin at centrosomes. leptomycin B 59-71 exportin 1 Homo sapiens 105-109 19117945-5 2009 Although all the mDia isoforms were localized predominantly in the cytoplasm under the steady-state conditions, mDia2 and not mDia1 or mDia3 accumulated extensively in the nucleus upon treatment with leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export. leptomycin B 200-212 exportin 1 Homo sapiens 236-240 19513080-6 2009 This export of HuR was not affected by at least 7 h of treatment of leptomycin B (LMB), which is an inhibitor of the CRM1-dependent export pathway. leptomycin B 82-85 exportin 1 Homo sapiens 117-121 19503814-6 2009 On the other hand, when active nuclear export was inhibited using leptomycin B, a specific inhibitor of the nuclear export receptor CRM1, both endogenous Atx3 and transfected GFP-Atx3 accumulated inside the nucleus of a subpopulation of COS-7 cells, whereas both proteins are normally predominant in the cytoplasm. leptomycin B 66-78 exportin 1 Homo sapiens 132-136 19297465-4 2009 Specific inhibition of Crm1-mediated nuclear export by leptomycin B increased M nuclear accumulation. leptomycin B 55-67 exportin 1 Homo sapiens 23-27 19536614-4 2009 The Rev NES receptor CRM1 (chromosome region maintenance 1)-specific inhibitor, leptomycin B, blocked luc+ mRNA export. leptomycin B 80-92 exportin 1 Homo sapiens 21-25 19220812-3 2009 Inhibition of CRM1-mediated nuclear export by Leptomycin B resulted in nuclear accumulation of COMMD1. leptomycin B 46-58 exportin 1 Homo sapiens 14-18 19321130-5 2009 Furthermore, we have shown that incubation of these cells with leptomycin B (LMB), a specific inhibitor of nuclear export mediated by chromosome region maintenance 1 (CRM1), leads to intranuclear accumulation of PLTP, suggesting that PLTP nuclear export is CRM1-dependent. leptomycin B 63-75 exportin 1 Homo sapiens 167-171 19321130-5 2009 Furthermore, we have shown that incubation of these cells with leptomycin B (LMB), a specific inhibitor of nuclear export mediated by chromosome region maintenance 1 (CRM1), leads to intranuclear accumulation of PLTP, suggesting that PLTP nuclear export is CRM1-dependent. leptomycin B 63-75 exportin 1 Homo sapiens 257-261 19321130-5 2009 Furthermore, we have shown that incubation of these cells with leptomycin B (LMB), a specific inhibitor of nuclear export mediated by chromosome region maintenance 1 (CRM1), leads to intranuclear accumulation of PLTP, suggesting that PLTP nuclear export is CRM1-dependent. leptomycin B 77-80 exportin 1 Homo sapiens 167-171 19321130-5 2009 Furthermore, we have shown that incubation of these cells with leptomycin B (LMB), a specific inhibitor of nuclear export mediated by chromosome region maintenance 1 (CRM1), leads to intranuclear accumulation of PLTP, suggesting that PLTP nuclear export is CRM1-dependent. leptomycin B 77-80 exportin 1 Homo sapiens 257-261 18978352-5 2009 Mutation of Ser-271 to Ala allowed nuclear export of 5-LO that was blocked by the specific nuclear export inhibitor leptomycin b, suggesting that phosphorylation of Ser-271 serves to interfere with exportin-1-mediated nuclear export. leptomycin B 116-128 exportin 1 Homo sapiens 198-208 19309550-8 2009 leptomycin B, an inhibitor of CRM1/exportin-1alpha-dependent nuclear export, reverted the inhibitory effect of ST1959 on NFAT1 nuclear localization. leptomycin B 0-12 exportin 1 Homo sapiens 30-34 18715912-4 2008 Finally, we show that the hUL47 NES is sensitive to the inhibitor of CRM1-mediated nuclear export leptomycin B. leptomycin B 98-110 exportin 1 Homo sapiens 69-73 18782761-6 2008 The NES in KLF5 directs a fused green fluorescence protein to the cytoplasm, binds the nuclear export receptor CRM1, and is inhibited by leptomycin and site-directed mutagenesis. leptomycin B 137-147 exportin 1 Homo sapiens 111-115 18817770-6 2008 Nuclear accumulation of Lyn was enhanced upon treatment of cells with leptomycin B (LMB), an inhibitor of Crm1-mediated nuclear export. leptomycin B 70-82 exportin 1 Homo sapiens 106-110 18817770-6 2008 Nuclear accumulation of Lyn was enhanced upon treatment of cells with leptomycin B (LMB), an inhibitor of Crm1-mediated nuclear export. leptomycin B 84-87 exportin 1 Homo sapiens 106-110