PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22555071-1 2012 [(18)F]fluoroethoxybenzovesamicol ([(18)F]FEOBV) is one of the most promising radioligands for imaging the vesicular ACh transporter (VAChT) with positron emission tomography (PET). fluoroethoxy-benzovesamicol 7-33 solute carrier family 18 member A3 Rattus norvegicus 107-132 22555071-1 2012 [(18)F]fluoroethoxybenzovesamicol ([(18)F]FEOBV) is one of the most promising radioligands for imaging the vesicular ACh transporter (VAChT) with positron emission tomography (PET). fluoroethoxy-benzovesamicol 7-33 solute carrier family 18 member A3 Rattus norvegicus 134-139 21391649-6 2011 By omitting iodoacetamide from the protocol and reducing sample preparation time, our mass spectrometry-based studies now confirm previous cell-based studies which showed that sulforaphane reacts with at least four cysteine residues of Keap1 including C151. sulforaphane 176-188 kelch like ECH associated protein 1 Homo sapiens 236-241 20486933-5 2010 To identify chemical modulator targeting the IVR domain of Keap1, we built a 3D structural model of the Keap1 IVR domain and demonstrated this structural model is effective in retrieving novel Nrf2 inducers from chemical databases, BM10, 31, and 40 increase concentration of nuclear Nrf2, with a potency comparable to that of sulforaphane. sulforaphane 326-338 kelch like ECH associated protein 1 Homo sapiens 59-64 20486933-5 2010 To identify chemical modulator targeting the IVR domain of Keap1, we built a 3D structural model of the Keap1 IVR domain and demonstrated this structural model is effective in retrieving novel Nrf2 inducers from chemical databases, BM10, 31, and 40 increase concentration of nuclear Nrf2, with a potency comparable to that of sulforaphane. sulforaphane 326-338 kelch like ECH associated protein 1 Homo sapiens 104-109 18407246-5 2008 Treatment of HK2 renal tubular epithelial cells with sulforaphane effectively protected cells against cytotoxicity induced by hypoxia-reoxygenation, and sulforaphane dramatically induced phase 2 enzymes by decreasing the Keap1 protein levels and increasing Nrf2 nuclear translocation. sulforaphane 153-165 kelch like ECH associated protein 1 Homo sapiens 221-226 18417180-9 2008 Furthermore, induction of Nrf2 by arsenic is independent of the previously identified C151 residue in Keap1 that is required for Nrf2 activation by tBHQ or SF. sulforaphane 156-158 kelch like ECH associated protein 1 Homo sapiens 102-107 16951197-1 2006 Exposure of sulforaphane to HepG2 cells increased heme oxygenase-1 (HO-1) expression by activating antioxidant response element (ARE) through induction of Nrf2 and suppression of Kelch-like ECH-associated protein 1 (Keap1). sulforaphane 12-24 kelch like ECH associated protein 1 Homo sapiens 179-214 17028159-2 2007 SFN has been promoted as a putative chemopreventive agent to reduce cancer, and most studies have associated its anti-cancer effects with the induction of phase II xenobiotic metabolism enzymes via activation of the Keap1/Nrf2 antioxidant response pathway. sulforaphane 0-3 kelch like ECH associated protein 1 Homo sapiens 216-221 17046835-6 2006 Quinone-induced oxidative stress and the chemopreventive agent sulforaphane inhibit Keap1-dependent ubiquitination of PGAM5. sulforaphane 63-75 kelch like ECH associated protein 1 Homo sapiens 84-89 16951197-1 2006 Exposure of sulforaphane to HepG2 cells increased heme oxygenase-1 (HO-1) expression by activating antioxidant response element (ARE) through induction of Nrf2 and suppression of Kelch-like ECH-associated protein 1 (Keap1). sulforaphane 12-24 kelch like ECH associated protein 1 Homo sapiens 216-221 16006525-4 2005 Chemical inducers such as sulforaphane are known to react with Keap1 cysteine residues, thereby promoting Nrf2 nuclear accumulation and hence ARE activation. sulforaphane 26-38 kelch like ECH associated protein 1 Homo sapiens 63-68 16520150-0 2006 Chemoprotection by sulforaphane: keep one eye beyond Keap1. sulforaphane 19-31 kelch like ECH associated protein 1 Homo sapiens 53-58 16520150-3 2006 Considerable attention has focused on SFN as a "blocking" agent, with the ability to modulate the Nrf2/Keap1 pathway, but recent evidence suggests that SFN acts by numerous other mechanisms. sulforaphane 38-41 kelch like ECH associated protein 1 Homo sapiens 103-108 16359182-0 2005 Identification of sensor cysteines in human Keap1 modified by the cancer chemopreventive agent sulforaphane. sulforaphane 95-107 kelch like ECH associated protein 1 Homo sapiens 44-49 16359182-2 2005 Sulforaphane exerts cancer chemopreventive effects by inducing antioxidant/electrophile response element (ARE)-regulated phase 2 enzyme and antioxidant genes through activation of the transcription factor nuclear factor-E2-related factor 2 (Nrf2), which is regulated by the thiol-rich sensor protein Kelch-like ECH-associated protein 1 (Keap1). sulforaphane 0-12 kelch like ECH associated protein 1 Homo sapiens 300-335 16359182-2 2005 Sulforaphane exerts cancer chemopreventive effects by inducing antioxidant/electrophile response element (ARE)-regulated phase 2 enzyme and antioxidant genes through activation of the transcription factor nuclear factor-E2-related factor 2 (Nrf2), which is regulated by the thiol-rich sensor protein Kelch-like ECH-associated protein 1 (Keap1). sulforaphane 0-12 kelch like ECH associated protein 1 Homo sapiens 337-342 16359182-4 2005 We hypothesized that, like other electrophilic Nrf2 activators, sulforaphane activates this system through specific modifications of the Keap1 protein. sulforaphane 64-76 kelch like ECH associated protein 1 Homo sapiens 137-142 16359182-5 2005 However, thionoacyl adducts are labile to hydrolysis and transacylation reactions, which complicate the identification of the sulforaphane adduct sites on Keap1. sulforaphane 126-138 kelch like ECH associated protein 1 Homo sapiens 155-160 16359182-6 2005 In this study, we characterized the stability of sulforaphane thionoacyl adducts and developed a liquid chromatography-tandem mass spectrometry method to map labile sulforaphane adduct sites formed on Keap1 in vitro. sulforaphane 165-177 kelch like ECH associated protein 1 Homo sapiens 201-206 16359182-7 2005 Sulforaphane displays a distinctly different pattern of Keap1 modification than previously studied ARE inducers that modify Keap1 by alkylation. sulforaphane 0-12 kelch like ECH associated protein 1 Homo sapiens 56-61 16359182-7 2005 Sulforaphane displays a distinctly different pattern of Keap1 modification than previously studied ARE inducers that modify Keap1 by alkylation. sulforaphane 0-12 kelch like ECH associated protein 1 Homo sapiens 124-129 16359182-8 2005 Sulforaphane modified Keap1 most readily in the Kelch domain, rather than in the central linker domain, which is targeted by previously characterized ARE inducers. sulforaphane 0-12 kelch like ECH associated protein 1 Homo sapiens 22-27 16359182-13 2005 Our observations suggest a novel mechanism for Nrf2 stabilization by sulforaphane-Keap1 thionoacyl adduct formation. sulforaphane 69-81 kelch like ECH associated protein 1 Homo sapiens 82-87 15826493-3 2005 SUL strongly induced Nrf2 protein expression and ARE-mediated transcription activation, retarded degradation of Nrf2 through inhibiting Keap1, and thereby activating the transcriptional expression of HO-1. sulforaphane 0-3 kelch like ECH associated protein 1 Homo sapiens 136-141 15923610-2 2005 In CaCo-2 cells, GI-GPx was induced by t-butyl hydroquinone (tBHQ) and sulforaphane (SFN), i.e., "antioxidants" known to activate the "antioxidant response element" (ARE) via electrophilic thiol modification of Keap1 in the Nrf2/Keap1 system. sulforaphane 71-83 kelch like ECH associated protein 1 Homo sapiens 211-216 15923610-2 2005 In CaCo-2 cells, GI-GPx was induced by t-butyl hydroquinone (tBHQ) and sulforaphane (SFN), i.e., "antioxidants" known to activate the "antioxidant response element" (ARE) via electrophilic thiol modification of Keap1 in the Nrf2/Keap1 system. sulforaphane 71-83 kelch like ECH associated protein 1 Homo sapiens 229-234 15923610-2 2005 In CaCo-2 cells, GI-GPx was induced by t-butyl hydroquinone (tBHQ) and sulforaphane (SFN), i.e., "antioxidants" known to activate the "antioxidant response element" (ARE) via electrophilic thiol modification of Keap1 in the Nrf2/Keap1 system. sulforaphane 85-88 kelch like ECH associated protein 1 Homo sapiens 211-216 15923610-2 2005 In CaCo-2 cells, GI-GPx was induced by t-butyl hydroquinone (tBHQ) and sulforaphane (SFN), i.e., "antioxidants" known to activate the "antioxidant response element" (ARE) via electrophilic thiol modification of Keap1 in the Nrf2/Keap1 system. sulforaphane 85-88 kelch like ECH associated protein 1 Homo sapiens 229-234 15923610-3 2005 The functional significance of a putative ARE in the GI-GPx promoter was validated by transcriptional activation of reporter gene constructs upon exposure to electrophiles (tBHQ, SFN, and curcumin) or overexpression of Nrf2 and by reversal of these effects by mutation of the ARE in the promoter and by overexpressed Keap1. sulforaphane 179-182 kelch like ECH associated protein 1 Homo sapiens 317-322 15572695-5 2004 Keap1-dependent ubiquitination of Nrf2 is inhibited following exposure of cells to quinone-induced oxidative stress and sulforaphane, a cancer-preventive isothiocyanate. sulforaphane 120-132 kelch like ECH associated protein 1 Homo sapiens 0-5 15572695-6 2004 A mutant Keap1 protein containing a single cysteine-to-serine substitution at residue 151 within the BTB domain of Keap1 is markedly resistant to inhibition by either quinone-induced oxidative stress or sulforaphane. sulforaphane 203-215 kelch like ECH associated protein 1 Homo sapiens 9-14 15572695-6 2004 A mutant Keap1 protein containing a single cysteine-to-serine substitution at residue 151 within the BTB domain of Keap1 is markedly resistant to inhibition by either quinone-induced oxidative stress or sulforaphane. sulforaphane 203-215 kelch like ECH associated protein 1 Homo sapiens 115-120 14585973-7 2003 Both sulforaphane, a chemopreventive isothiocyanate, and oxidative stress enable Nrf2 to escape Keap1-dependent degradation, leading to stabilization of Nrf2, increased nuclear localization of Nrf2, and activation of Nrf2-dependent cancer-protective genes. sulforaphane 5-17 kelch like ECH associated protein 1 Homo sapiens 96-101 14585973-8 2003 We have identified a third cysteine residue in Keap1, C151, that is uniquely required for inhibition of Keap1-dependent degradation of Nrf2 by sulforaphane and oxidative stress. sulforaphane 143-155 kelch like ECH associated protein 1 Homo sapiens 47-52 14585973-8 2003 We have identified a third cysteine residue in Keap1, C151, that is uniquely required for inhibition of Keap1-dependent degradation of Nrf2 by sulforaphane and oxidative stress. sulforaphane 143-155 kelch like ECH associated protein 1 Homo sapiens 104-109 35041848-3 2022 Accumulating evidence suggest that signaling between Kelch-like erythroid cell-derived protein with CNC homology (ECH)-associated protein 1 (Keap1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2) plays an important role in the pathogenesis and treatment of brain dysfunction, while sulforaphane (SFN), a natural compound acting as an Nrf2 agonist, can improve brain function. sulforaphane 289-301 kelch like ECH associated protein 1 Homo sapiens 141-146