PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20112680-3 2009 The recombinant protein (Nsp2-N and Nsp2-C) were over expressed in E. coli BL21 and purified by Ni-NTA agarose affinity chromatogram and gel filtration. ni-nta 96-102 reticulon 2 Homo sapiens 25-29