PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24599317-7 2014 Treatments increasing nitrite production also increased iNOS expression, while treatments decreasing nitrite production reduced iNOS expression. Nitrites 101-108 nitric oxide synthase 2, inducible Mus musculus 128-132 27526131-5 2016 Acidotic conditions for up to 6 h increased the iNOS expression significantly which was functional as indicated by an elevated level of nitrate/nitrite formation by 30 %. Nitrites 144-151 nitric oxide synthase 2, inducible Mus musculus 48-52 24599317-7 2014 Treatments increasing nitrite production also increased iNOS expression, while treatments decreasing nitrite production reduced iNOS expression. Nitrites 22-29 nitric oxide synthase 2, inducible Mus musculus 56-60 24599317-8 2014 Among the different NOS isoforms, experiments using L-748,337 or BRL37344 with activators or inhibitors targeting specific NOS isoforms demonstrated a prominent role of iNOS in nitrite production. Nitrites 177-184 nitric oxide synthase 2, inducible Mus musculus 169-173 24494186-8 2014 Nitrite also displayed therapeutic effects by ameliorating established colonic inflammation with reduced colonic expression of iNOS and improving histopathology. Nitrites 0-7 nitric oxide synthase 2, inducible Mus musculus 127-131 24645669-10 2014 TUDCA treatment reduced the production of nitrites by microglial cells and astrocytes induced by proinflammatory stimuli that led to transcriptional and translational diminution of the iNOS. Nitrites 42-50 nitric oxide synthase 2, inducible Mus musculus 185-189 23373571-7 2013 KEY RESULTS: LPS caused a significant production of nitrites, associated to up-regulation of anandamide, iNOS, COX-2, CB1 receptors and down-regulation of CB2 receptors mRNA expression. Nitrites 52-60 nitric oxide synthase 2, inducible Mus musculus 105-109 24145454-8 2013 Thus, whether or not Mtb is hypoxic, the host expresses iNOS, or hypoxia impairs the action of iNOS, Mtb in vivo is likely to encounter RNS by producing nitrite. Nitrites 153-160 nitric oxide synthase 2, inducible Mus musculus 95-99 23609920-11 2013 CONCLUSIONS: These results suggest that exendin-4 protects against IL-1beta- induced apoptosis in beta-cells via downregulation of the NF- kappaB-iNOS-nitrite pathway. Nitrites 151-158 nitric oxide synthase 2, inducible Mus musculus 146-150 23946691-3 2013 Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. Nitrites 0-7 nitric oxide synthase 2, inducible Mus musculus 237-241 23441627-8 2013 The high NOS-2 expression coincided with an exacerbated NO production in the infection focus and in plasma, as judging by nitrate + nitrite levels. Nitrites 132-139 nitric oxide synthase 2, inducible Mus musculus 9-14 22537094-4 2012 When RAW264.7 cells and primary macrophages were preincubated with 11-oxoaerothionin and stimulated with LPS (lipopolysaccharide), a concentration-dependent inhibition of iNOS(inducible nitric oxide synthase) protein and NO(2)(-) (Nitrite) production were observed. Nitrites 231-238 nitric oxide synthase 2, inducible Mus musculus 171-175 17056198-5 2006 This caused up to 2.1-fold radiosensitization of EMT-6 tumor cells, which was associated with the iNOS-mediated production of the radiosensitizing molecule nitric oxide, as confirmed by accumulation of its oxidative metabolite nitrite, Western blot analysis, and reverse transcriptase-polymerase chain reaction. Nitrites 227-234 nitric oxide synthase 2, inducible Mus musculus 98-102 22819108-4 2012 In vitro inducible nitric oxide synthase activity, determined as nitrite, was higher in MB49-I than in MB49 cells (p <0.001). Nitrites 65-72 nitric oxide synthase 2, inducible Mus musculus 9-40 21554376-9 2011 Levels of nitrite and nitrate (NO(x)), as an index of nitric oxide, bioavailability were significantly decreased in the iNOS(-/-) diabetic mouse heart. Nitrites 10-17 nitric oxide synthase 2, inducible Mus musculus 120-124 20388518-10 2010 This up-regulation of nitrites generation also correlated with the induction of inducible nitric oxide synthase (iNOS), a prominent marker of macrophage activation. Nitrites 22-30 nitric oxide synthase 2, inducible Mus musculus 80-111 20388518-10 2010 This up-regulation of nitrites generation also correlated with the induction of inducible nitric oxide synthase (iNOS), a prominent marker of macrophage activation. Nitrites 22-30 nitric oxide synthase 2, inducible Mus musculus 113-117 19001185-13 2009 However, in mice lacking iNOS, maximum and minimum dP/dt, as well as an indicator of isovolumic contraction, markedly increased in response to isoproterenol, associated with decreased cardiac 4-hydroxy-2-nonenal expression and urinary nitrate/nitrite. Nitrites 243-250 nitric oxide synthase 2, inducible Mus musculus 25-29 20399279-8 2010 Oral nitrite supplementation to colitis mice reversed colonic nitrite levels and TNF-alpha expression to that of normal control mice at day 3, resulting in the reduction of MPO activity as well as iNOS and HO-1 expressions in colonic tissues with clinical and histological improvements at day 7. Nitrites 5-12 nitric oxide synthase 2, inducible Mus musculus 197-201 19121076-6 2009 Bone marrow-derived macrophages from the knockout mice killed C. albicans yeasts efficiently in vitro, and were still able to produce nitrites in an iNOS-independent manner, albeit less efficiently than wild-type controls. Nitrites 134-142 nitric oxide synthase 2, inducible Mus musculus 149-153 18394025-9 2008 RESULTS: Despite similar infarct size, deficiency in iNOS resulted in significantly lower plasma nitrate/nitrite levels, better haemodynamic performance and lower mortality 2 weeks after coronary ligation. Nitrites 105-112 nitric oxide synthase 2, inducible Mus musculus 53-57 17530864-5 2007 Nitrite ion rapidly accumulated in the extracellular medium over a period of 5-8 h post-activation in both cell lines, indicating the presence of active nitric oxide synthase (iNOS) during that period. Nitrites 0-7 nitric oxide synthase 2, inducible Mus musculus 176-180 17023389-3 2006 We created mouse embryo fibroblast (MEF) cell lines that would facilitate investigation of the effect of mPGES-1 genetic deletion on prostanoid biosynthesis in fibroblast lineage cells and its subsequent effect on the expression of inducible NOS (iNOS) and nitrite biosynthesis using cells derived from mPGES-1 wild-type (WT), heterozygous (Het), and null mice. Nitrites 257-264 nitric oxide synthase 2, inducible Mus musculus 232-245 16473374-3 2006 During our study, we investigated the effects of azathioprine on the inducible nitric oxide synthase (iNOS) in lipopolysaccharide stimulated murine macrophages (RAW 264.7) by measurement of iNOS protein (immunoblotting), iNOS mRNA (semiquantitative competitive RT-PCR), and NO production (nitrite levels). Nitrites 289-296 nitric oxide synthase 2, inducible Mus musculus 102-106 16705756-4 2006 Nitrite plus nitrate levels were lower in iNOS(-/-) compared with iNOS(+/+) mice, but CCl(4) did not produce a significant effect in any mice. Nitrites 0-7 nitric oxide synthase 2, inducible Mus musculus 42-46 15902277-8 2005 A single injection of 25 microg of pE9iNOS DNA in a lipid vector into the centre of a murine sarcoma (RIF1) induced iNOS protein expression by four-fold and increased nitrite concentration eight-fold. Nitrites 167-174 nitric oxide synthase 2, inducible Mus musculus 38-42 16771678-4 2006 Neuronal NOS2 was assessed by increases in nitrite accumulation, and increases in NOS2 mRNA levels and fluorescence of the NO-sensitive probe DAF-2 DA. Nitrites 43-50 nitric oxide synthase 2, inducible Mus musculus 9-13 16771679-10 2006 These data suggest that the human-specific release of nanomolar levels of nitrite may largely result from differences between the human and mouse NOS2 genes, which may program different degrees of nitric oxide responses to inflammatory signals in humans than in mice. Nitrites 74-81 nitric oxide synthase 2, inducible Mus musculus 146-150 16601319-3 2006 Pretreatment with ISO (1-300 microM) resulted in dose-dependent inhibition of accumulation of NOS-2-derived nitrite in culture medium (IC(50) = 9,3 microM) as well as inhibition of NOS-2 protein induction in cultured J774.2 cells; ISO given 10 hours after LPS did not influence activity of NOS-2. Nitrites 108-115 nitric oxide synthase 2, inducible Mus musculus 94-99 16223598-4 2006 The activity of mtNOS and nitrite levels significantly increased after sepsis in iNOS+/+ mice. Nitrites 26-33 nitric oxide synthase 2, inducible Mus musculus 81-85 14607816-2 2004 C57BL/6 mice injected intraperitoneally with the specific inducible NO synthase (iNOS) inhibitor 1400W (10 mg/kg every 8 h for 72 h) exhibited decreased alveolar nitrite levels and Na(+)-dependent amiloride-sensitive alveolar fluid clearance as compared with mice injected with vehicle. Nitrites 162-169 nitric oxide synthase 2, inducible Mus musculus 81-85 12628482-4 2003 At the same time, low levels of nitrite accumulated in the culture medium and expression of nitric oxide synthase-2 (NOS-2) and NOS-3 protein was detected. Nitrites 32-39 nitric oxide synthase 2, inducible Mus musculus 92-115 15028566-6 2003 NOS-2 activity was assessed after 24 hours by nitrite accumulation in the culture media. Nitrites 46-53 nitric oxide synthase 2, inducible Mus musculus 0-5 12839867-6 2003 In differentiated 3T3-F442A, treatment with TNFalpha (20 ng ml(-1)) induced the expression of a functional iNOS as demonstrated by nitrite assay, Western blot, reverse transcription-polymerase chain reaction and Northern blot analysis. Nitrites 131-138 nitric oxide synthase 2, inducible Mus musculus 107-111 12628482-4 2003 At the same time, low levels of nitrite accumulated in the culture medium and expression of nitric oxide synthase-2 (NOS-2) and NOS-3 protein was detected. Nitrites 32-39 nitric oxide synthase 2, inducible Mus musculus 117-122 12237746-8 2002 In mice treated with LPS significantly increased levels of plasma nitrite and serum TNF-alpha were observed, changes inhibited by aminoguanidine [an inhibitor of inducible NO synthase (iNOS)] and pentoxifylline (an inhibitor of tumor necrosis factor-alpha formation), respectively. Nitrites 66-73 nitric oxide synthase 2, inducible Mus musculus 162-183 12563676-10 2003 The serum nitrite/nitrate levels, histological grades of articular cartilage degradation, and numbers of apoptotic chondrocytes and nitrotyrosine positive chondrocytes were significantly lower in NOS2-/- mice with AMA than in WT mice with AMA. Nitrites 10-17 nitric oxide synthase 2, inducible Mus musculus 196-200 12494944-3 2003 The nitrite levels in urine of iNOS+/+ but not iNOS/ mice increased after infection. Nitrites 4-11 nitric oxide synthase 2, inducible Mus musculus 31-35 12237746-8 2002 In mice treated with LPS significantly increased levels of plasma nitrite and serum TNF-alpha were observed, changes inhibited by aminoguanidine [an inhibitor of inducible NO synthase (iNOS)] and pentoxifylline (an inhibitor of tumor necrosis factor-alpha formation), respectively. Nitrites 66-73 nitric oxide synthase 2, inducible Mus musculus 185-189 11793130-6 2002 Urinary nitrite + nitrate excretion was significantly lower in iNOS KO mice compared to control animals at all time points; in C57 mice, urinary nitrite declined progressively with more prolonged duration of diabetes. Nitrites 8-15 nitric oxide synthase 2, inducible Mus musculus 63-67 12358336-9 2002 In iNOS-deficient mice, both iNOS expression and NT formation were completely abolished, and the total amounts of nitrite and nitrate in BAL fluid were significantly decreased. Nitrites 114-121 nitric oxide synthase 2, inducible Mus musculus 3-7 12127127-9 2002 Plasma and intestinal levels of the nitric oxide products, nitrite/nitrate, were increased in the iNOS +/+ mice fed the TPN solution but not in the chow-fed groups or the iNOS -/- mice receiving TPN solution. Nitrites 59-66 nitric oxide synthase 2, inducible Mus musculus 98-102 12183564-3 2002 Immunoblotting and nitrite assays revealed that C. burnetii infection of L-929 cells augments expression of iNOS up-regulated in response to gamma interferon (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha). Nitrites 19-26 nitric oxide synthase 2, inducible Mus musculus 108-112 12183564-5 2002 Nitrite production by cytokine-treated cells was significantly inhibited by the iNOS inhibitor S-methylisothiourea (SMT). Nitrites 0-7 nitric oxide synthase 2, inducible Mus musculus 80-84 12064473-4 2002 The production of nitrite peaked after 48 h of incubation, and this reflected transcriptional activation of the NOS-2 gene and increased expression of the NOS-2 protein. Nitrites 18-25 nitric oxide synthase 2, inducible Mus musculus 112-117 12064473-4 2002 The production of nitrite peaked after 48 h of incubation, and this reflected transcriptional activation of the NOS-2 gene and increased expression of the NOS-2 protein. Nitrites 18-25 nitric oxide synthase 2, inducible Mus musculus 155-160 11442316-2 2001 A severe inflammatory response characterized by peritoneal exudation, high peritoneal levels of nitrate/nitrite, and leukocyte infiltration into peritoneal exudate was induced by zymosan administration in iNOS +/+ mice. Nitrites 104-111 nitric oxide synthase 2, inducible Mus musculus 205-209 11564662-6 2001 The in vivo intravenous administration of aODNs to iNOS, 24 and 12 h before murine tumour necrosis factor alpha (mTNFalpha) challenge, significantly reduced the nitrite levels induced by the mTNFalpha challenge. Nitrites 161-168 nitric oxide synthase 2, inducible Mus musculus 51-55 11442316-5 2001 Peritoneal administration of zymosan in the iNOS +/+ mice induced also a significant increase in the plasma levels of nitrite/nitrate and in the levels of peroxynitrite at 18 h after zymosan challenge. Nitrites 118-125 nitric oxide synthase 2, inducible Mus musculus 44-48 11407704-8 2001 RESULTS: In iNOS+/+ animals with AIA, the plasma concentration of nitrite/nitrate was increased 3-fold and iNOS expression was detected in cells of the joint. Nitrites 66-73 nitric oxide synthase 2, inducible Mus musculus 12-16 11240902-6 2001 Nitrite production in culture supernatants was due to induction of isoform NOS-2 because both NG monomethyl L-arginine (100 microM) and dexamethasone (20 microM) inhibited, by 60 and 50%, respectively, nitrite accumulation in culture supernatants. Nitrites 0-7 nitric oxide synthase 2, inducible Mus musculus 75-80 11240902-6 2001 Nitrite production in culture supernatants was due to induction of isoform NOS-2 because both NG monomethyl L-arginine (100 microM) and dexamethasone (20 microM) inhibited, by 60 and 50%, respectively, nitrite accumulation in culture supernatants. Nitrites 202-209 nitric oxide synthase 2, inducible Mus musculus 75-80 10906155-7 2000 The GH-induced increase in iNOS transcript levels was accompanied by a significant increase in nitrite concentrations in conditioned media, which was blocked by the addition of L-N(G)-monomethylarginine. Nitrites 95-102 nitric oxide synthase 2, inducible Mus musculus 27-31 11067741-7 2000 Stable expression of NOS II was assessed by measuring nitrite accumulation in media every 2 days. Nitrites 54-61 nitric oxide synthase 2, inducible Mus musculus 21-27 11098975-13 2000 iNOS+/+ mice subjected to SMAO had increased plasma concentrations of nitrite (NO2-) and nitrate (NO3-), and the plasma concentrations of NO2- and NO3- were highest in the mice in which bacterial translocation had occurred. Nitrites 70-77 nitric oxide synthase 2, inducible Mus musculus 0-4 11020457-5 2000 The polyphenols quercetin and resveratrol at a micromolar range suppressed iNOS gene expression and NO production, as determined by reverse transcription-polymerase chain reaction and nitrite assay. Nitrites 184-191 nitric oxide synthase 2, inducible Mus musculus 75-79 10944420-3 2000 IFN-gamma alone or combined with LPS induced iNOS expression and increased nitrite production in iNOS(+/+) macrophages, but not in iNOS(-/-) macrophages. Nitrites 75-82 nitric oxide synthase 2, inducible Mus musculus 97-101 10944420-3 2000 IFN-gamma alone or combined with LPS induced iNOS expression and increased nitrite production in iNOS(+/+) macrophages, but not in iNOS(-/-) macrophages. Nitrites 75-82 nitric oxide synthase 2, inducible Mus musculus 97-101 10788454-3 2000 Total nitrate and nitrite production was completely abolished in cells from iNOS-deficient animals compared with control cells. Nitrites 18-25 nitric oxide synthase 2, inducible Mus musculus 76-80 10923019-9 2000 Wound fluid nitrite/nitrate levels were higher in WT than iNOS-KO animals but were not significantly influenced by additional arginine. Nitrites 12-19 nitric oxide synthase 2, inducible Mus musculus 58-62 10432387-4 1999 METHODS: Expression of iNOS was evaluated by NO production (nitrite analysis), protein (Western blot analysis) and mRNA (RT-PCR analysis) levels in mesangial cells stimulated by a combination of lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) in the presence and absence of all-trans-retinoic acid (ATRA) or its active metabolite, 13-cis-retinoic acid (13-cis-RA). Nitrites 60-67 nitric oxide synthase 2, inducible Mus musculus 23-27 9614147-6 1998 We show that inhibition of NF-kappaB by pyrrolidinedithiocarbamate prevents poly(I-C) + IFN-gamma-, poly(I-C) + LPS-, and LPS-induced iNOS expression, nitrite production and IkappaB degradation by RAW 264.7 cells. Nitrites 151-158 nitric oxide synthase 2, inducible Mus musculus 134-138 10231435-9 1999 The combination of the LPS/IFN-gamma mixture induced iNOS expression and NO production in murine mesangial cells, as assessed by Western blot analysis and measurement of nitrite levels. Nitrites 170-177 nitric oxide synthase 2, inducible Mus musculus 53-57 10079054-2 1999 The decrease in NO, as demonstrated by measurement of nitrite was found to correlate well with a decrease in inducible nitric oxide synthase (iNOS) mRNA. Nitrites 54-61 nitric oxide synthase 2, inducible Mus musculus 109-140 10079054-2 1999 The decrease in NO, as demonstrated by measurement of nitrite was found to correlate well with a decrease in inducible nitric oxide synthase (iNOS) mRNA. Nitrites 54-61 nitric oxide synthase 2, inducible Mus musculus 142-146 9806822-3 1998 Aminoguanidine, a selective inducible NO synthase (iNOS) inhibitor, at a dose of 100 mg kg-1 significantly reduced the LPS-induced increase in nitrite levels and improved mortality. Nitrites 143-150 nitric oxide synthase 2, inducible Mus musculus 28-49 9806822-3 1998 Aminoguanidine, a selective inducible NO synthase (iNOS) inhibitor, at a dose of 100 mg kg-1 significantly reduced the LPS-induced increase in nitrite levels and improved mortality. Nitrites 143-150 nitric oxide synthase 2, inducible Mus musculus 51-55 9806822-4 1998 Econazole, iNOS inhibitor, calmodulin antagonist, 5-lipoxygenase and a specific thromboxane synthase inhibitor, at a 1 mg kg-1 dose significantly decreased the LPS-induced increase in nitrite levels, but increased mortality 4. Nitrites 184-191 nitric oxide synthase 2, inducible Mus musculus 11-15 9806822-6 1998 Indomethacin, a putative iNOS and non-selective cyclo-oxygenase (COX) inhibitor, of 1, 10 and 100 mg kg-1, dose dependently decreased the LPS-induced increase in nitrite levels. Nitrites 162-169 nitric oxide synthase 2, inducible Mus musculus 25-29 9652371-6 1998 The reduction in lipopolysaccharide-stimulated nitrite accumulation produced by tetracyclines was significantly less when they were applied 6 h after lipopolysaccharide and absent 12 h after lipopolysaccharide, indicating that tetracyclines modify an early event in inducible NO synthase activation operating after mRNA transcription. Nitrites 47-54 nitric oxide synthase 2, inducible Mus musculus 266-287 9450620-2 1997 We report here that proteins recovered from the medium of dexamethasone-treated J774 macrophages (1, 10, 100 microg/ml) inhibited lipopolysaccharide-stimulated nitrite generation by 10.0 +/- 3.0%, 32.3 +/- 5.3% and 55.0 +/- 6.0%, respectively, and inducible NO synthase mRNA expression in these cells. Nitrites 160-167 nitric oxide synthase 2, inducible Mus musculus 248-269 9659467-7 1998 Enhanced nitrite production by coculture was accompanied by increased inducible NO synthase protein accumulation. Nitrites 9-16 nitric oxide synthase 2, inducible Mus musculus 70-91 9394817-3 1997 Inducible NO synthase (iNOs) expression was not evidenced in non-stimulated MMC obtained by culture of hematopoietic progenitors in the presence of interleukin-3, whereas IgE-antigen-stimulated MMC expressed iNOs mRNA and protein and synthesized nitrites. Nitrites 246-254 nitric oxide synthase 2, inducible Mus musculus 0-21 9427324-3 1997 The i-NOS inhibitor L-N6-(1-iminoethyl)-lysine (NIL, 600 microM) suppressed the nitrite accumulation and the increase in Mn-SOD-like immunoreactivity in activated cells without significant effect on the level of i-NOS-like immunoreactivity. Nitrites 80-87 nitric oxide synthase 2, inducible Mus musculus 4-9 9366711-9 1997 Nitrite and nitrate levels of the liver, ileum, and blood were higher in the iNOS+/+ mice (P < .05). Nitrites 0-7 nitric oxide synthase 2, inducible Mus musculus 77-81 9394817-3 1997 Inducible NO synthase (iNOs) expression was not evidenced in non-stimulated MMC obtained by culture of hematopoietic progenitors in the presence of interleukin-3, whereas IgE-antigen-stimulated MMC expressed iNOs mRNA and protein and synthesized nitrites. Nitrites 246-254 nitric oxide synthase 2, inducible Mus musculus 23-27 10851463-5 1997 Functional iNOS protein expression was confirmed by bioassay of nitrite accumulation in the culture supernatant. Nitrites 64-71 nitric oxide synthase 2, inducible Mus musculus 11-15 9242464-5 1997 Inhibition of iNOS in vivo was confirmed by decreases in plasma nitrite + nitrate concentrations in treated animals compared with that of controls (63-83% decreases for all experiments) and was supported by plasma and tumor concentrations of 1400W that were equivalent and 2.6-4.9 times higher than the EC50 previously reported for iNOS in a tissue assay. Nitrites 64-71 nitric oxide synthase 2, inducible Mus musculus 14-18 9174292-5 1997 The resultant iNOS expression was measured by using Northern blot analysis and cell supernatant nitrite concentrations (in aqueous media, NO is oxidized primarily to nitrite, NO2-) by chemiluminescence. Nitrites 96-103 nitric oxide synthase 2, inducible Mus musculus 14-18 9174292-5 1997 The resultant iNOS expression was measured by using Northern blot analysis and cell supernatant nitrite concentrations (in aqueous media, NO is oxidized primarily to nitrite, NO2-) by chemiluminescence. Nitrites 166-173 nitric oxide synthase 2, inducible Mus musculus 14-18 8980906-2 1996 Activation of the cells with lipopolysaccharide plus interferon-gamma (LI) induced iNOS, detected by nitrite or by labeled L-citrulline production and by a specific antibody against macrophage iNOS. Nitrites 101-108 nitric oxide synthase 2, inducible Mus musculus 83-87 8702745-8 1996 Similarly, expression of the inducible NO synthase in response to lipopolysaccharide and interferon-gamma also caused apoptosis in RAW macrophages and neo controls within 24 h. In contrast, Bcl-2 transfectants appeared highly resistant, although inducible NO synthase levels increased along with concomitant nitrite production similar to control cells. Nitrites 308-315 nitric oxide synthase 2, inducible Mus musculus 29-50 8898115-2 1996 The decreased nitrite formation was due not to the inhibition of nitric oxide synthase activity but to suppression of NOS II mRNA and protein expression. Nitrites 14-21 nitric oxide synthase 2, inducible Mus musculus 118-124 8613229-3 1996 When myocytes were treated with interleukin-1beta (5 ng/mL), nitrite levels increased, and this effect was inhibited 80% by the specific iNOS inhibitor aminoguanidine. Nitrites 61-68 nitric oxide synthase 2, inducible Mus musculus 137-141 8882611-4 1996 LTA (10 microgram ml-1) caused within 24 h an enhanced accumulation of nitrite (an indicator of NO biosynthesis) in the supernatant of J774.2 macrophages which was prevented by the non-selective NOS inhibitor NG-monomethyl-L-arginine (L-NMMA; IC50: 35 microM) or by the iNOS-selective NOS inhibitor, aminoethyl-isothiourea (AE-ITU; IC50: 6 microM). Nitrites 71-78 nitric oxide synthase 2, inducible Mus musculus 270-274 35221497-8 2022 Furthermore, TUNEL-positive cells were observed in granulosa cells of atretic follicles, and overexpression of caspase 8, c-Fos, and inducible nitric oxide synthase (iNOS) was detected in ovaries after nitrite exposure (p<0.01), suggesting that cell apoptosis and oxidative stress response were induced following nitrite exposure. Nitrites 202-209 nitric oxide synthase 2, inducible Mus musculus 166-170 8815312-5 1996 The appearance of i-NOS mRNA after endotoxin treatment of BV-2 cells was confirmed by Northern blot analysis and in situ hybridization histochemistry, and functional enzyme activity was followed by release of nitric oxide (as nitrite) into the medium. Nitrites 226-233 nitric oxide synthase 2, inducible Mus musculus 18-23 7500055-9 1995 Incubation of OA cartilage in serum-free medium resulted in spontaneous release, for up to 72 h, of substantial amounts of nitrite (up to approximately 80 microM/100 mg wet tissue), which could be inhibited by at least 80% with various inhibitors of iNOS, including inhibitors of protein synthesis and transcription factor NF-kappa B, but which (unlike murine macrophage iNOS) was not sensitive to hydrocortisone or TGF-beta. Nitrites 123-130 nitric oxide synthase 2, inducible Mus musculus 250-254 7500055-9 1995 Incubation of OA cartilage in serum-free medium resulted in spontaneous release, for up to 72 h, of substantial amounts of nitrite (up to approximately 80 microM/100 mg wet tissue), which could be inhibited by at least 80% with various inhibitors of iNOS, including inhibitors of protein synthesis and transcription factor NF-kappa B, but which (unlike murine macrophage iNOS) was not sensitive to hydrocortisone or TGF-beta. Nitrites 123-130 nitric oxide synthase 2, inducible Mus musculus 371-375 7536776-6 1995 iNOS mRNA was expressed maximally 6 h after stimulation with S. dublin, whereas maximal nitrite accumulation in supernatants was measured at 24 to 48 h. Significant differences with regard to cytokine regulation of iNOS were observed between murine and bovine macrophages cultured under identical conditions. Nitrites 88-95 nitric oxide synthase 2, inducible Mus musculus 215-219 7686937-6 1993 Suppression of nitrite accumulation and iNOS enzyme activity by prior exposure of macrophages to LPS could be explained by their markedly decreased content of iNOS protein, as revealed by immunoblot with monospecific anti-iNOS IgG. Nitrites 15-22 nitric oxide synthase 2, inducible Mus musculus 159-163 7686937-6 1993 Suppression of nitrite accumulation and iNOS enzyme activity by prior exposure of macrophages to LPS could be explained by their markedly decreased content of iNOS protein, as revealed by immunoblot with monospecific anti-iNOS IgG. Nitrites 15-22 nitric oxide synthase 2, inducible Mus musculus 159-163 7544010-2 1995 We report that exposure of lipopolysaccharide-stimulated murine macrophages to therapeutic concentrations of aspirin (IC50 = 3 mM) and hydrocortisone (IC50 = 5 microM) inhibited the expression of iNOS and production of nitrite. Nitrites 219-226 nitric oxide synthase 2, inducible Mus musculus 196-200 7537467-3 1995 The induction of nitrite production and iNOS mRNA was time dependent, requiring approximately 8 h for expression of significant levels of nitrite or iNOS mRNA. Nitrites 17-24 nitric oxide synthase 2, inducible Mus musculus 149-153 7537467-3 1995 The induction of nitrite production and iNOS mRNA was time dependent, requiring approximately 8 h for expression of significant levels of nitrite or iNOS mRNA. Nitrites 138-145 nitric oxide synthase 2, inducible Mus musculus 40-44 7531975-4 1995 Protein kinase C activation led to enhanced mRNA expression of iNOS, and inhibitors of protein kinase C blocked nitrite accumulation in mesangial cells. Nitrites 112-119 nitric oxide synthase 2, inducible Mus musculus 63-67 35221497-8 2022 Furthermore, TUNEL-positive cells were observed in granulosa cells of atretic follicles, and overexpression of caspase 8, c-Fos, and inducible nitric oxide synthase (iNOS) was detected in ovaries after nitrite exposure (p<0.01), suggesting that cell apoptosis and oxidative stress response were induced following nitrite exposure. Nitrites 313-320 nitric oxide synthase 2, inducible Mus musculus 166-170 32806494-0 2020 Systemic Insulin Resistance and Metabolic Perturbations in Chow Fed Inducible Nitric Oxide Synthase Knockout Male Mice: Partial Reversal by Nitrite Supplementation. Nitrites 140-147 nitric oxide synthase 2, inducible Mus musculus 68-99 32806494-9 2020 Obesity, gluconeogenesis, and adipose tissue insulin signaling were only partially reversed in nitrite supplemented iNOS-/- mice. Nitrites 95-102 nitric oxide synthase 2, inducible Mus musculus 116-120 32806494-10 2020 Our results thus demonstrate that nitrite supplementation to iNOS-/- mice improves insulin sensitivity and metabolic homeostasis, thus further highlighting the metabolic role of iNOS. Nitrites 34-41 nitric oxide synthase 2, inducible Mus musculus 61-65 32806494-10 2020 Our results thus demonstrate that nitrite supplementation to iNOS-/- mice improves insulin sensitivity and metabolic homeostasis, thus further highlighting the metabolic role of iNOS. Nitrites 34-41 nitric oxide synthase 2, inducible Mus musculus 178-182 29145236-9 2017 CONCLUSION: These results suggest that nitrite show certain protective effects against the ischemic effects induced by AMI in mice, which might be attributed to the synthesis of NO induced by iNOS through up-regulation of antiapoptotic factors and down-regulation of proapoptotic and inflammatory factors. Nitrites 39-46 nitric oxide synthase 2, inducible Mus musculus 192-196 28289922-10 2017 Significant inhibition in mRNA expression of iNOS, TGF-beta1 and TNF-alpha level was noted after curcumin treatment along with lowered MPO activity, inflammatory cell count, ROS, nitrite levels and collagen deposition in lungs. Nitrites 179-186 nitric oxide synthase 2, inducible Mus musculus 45-49 27623091-9 2016 Moreover, these improvement effects of methylphenidate on SAB and the nitrite level were decreased by the administration of selective iNOS and eNOS inhibitors. Nitrites 70-77 nitric oxide synthase 2, inducible Mus musculus 134-138