PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 28547849-4 2017 We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner. Heparitin Sulfate 92-107 fibroblast growth factor 2 Homo sapiens 53-79 33146178-1 2020 To map the cellular topography of the rare 3-O-sulfated structural motif of heparan sulfate (HS), we constructed quantum dot-based probes for antithrombin and FGF2, which reveal widely different distribution of the targeted HS motifs. Heparitin Sulfate 76-91 fibroblast growth factor 2 Homo sapiens 159-163 33146178-1 2020 To map the cellular topography of the rare 3-O-sulfated structural motif of heparan sulfate (HS), we constructed quantum dot-based probes for antithrombin and FGF2, which reveal widely different distribution of the targeted HS motifs. Heparitin Sulfate 93-95 fibroblast growth factor 2 Homo sapiens 159-163 30470711-3 2019 This process was dependent on both PI(4,5)P2-mediated recruitment of FGF2 at the inner leaflet and heparan sulfates capturing FGF2 at the outer plasma membrane leaflet. Heparitin Sulfate 99-115 fibroblast growth factor 2 Homo sapiens 126-130 30168023-11 2019 Binding of TSP-2 to FGF2 impaired the growth factor ability to interact with its cellular receptors, since TSP-2-derived fragments prevented the binding of FGF2 to both heparin (used as a structural analog of heparan sulfate proteoglycans) and FGFR-1. Heparitin Sulfate 209-224 fibroblast growth factor 2 Homo sapiens 20-24 30239102-2 2019 Disaccharide analogues of heparan sulfate had previously been identified as the shortest components out of the sugars that bind to FGF-1 and FGF-2. Heparitin Sulfate 26-41 fibroblast growth factor 2 Homo sapiens 141-146 28799166-5 2017 Membrane-inserted FGF2 oligomers are dynamic translocation intermediates that are disassembled at the extracellular leaflet mediated by membrane proximal heparan sulphate proteoglycans. Heparitin Sulfate 154-170 fibroblast growth factor 2 Homo sapiens 18-22 2466852-6 1989 Heparin sulfate (10 micrograms/ml) dramatically inhibited the ability of bFGF to stimulate the proliferation of keratinocytes. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 73-77 33159882-7 2020 As heparan sulfate modulates FGF-mediated signaling, we found a significantly decreased activation of the MAP kinases ERK1/2 in FGF-2-stimulated cell lines of affected individuals that could be restored by addition of heparin, a GAG similar to heparan sulfate. Heparitin Sulfate 3-18 fibroblast growth factor 2 Homo sapiens 29-32 33159882-7 2020 As heparan sulfate modulates FGF-mediated signaling, we found a significantly decreased activation of the MAP kinases ERK1/2 in FGF-2-stimulated cell lines of affected individuals that could be restored by addition of heparin, a GAG similar to heparan sulfate. Heparitin Sulfate 3-18 fibroblast growth factor 2 Homo sapiens 128-133 33159882-7 2020 As heparan sulfate modulates FGF-mediated signaling, we found a significantly decreased activation of the MAP kinases ERK1/2 in FGF-2-stimulated cell lines of affected individuals that could be restored by addition of heparin, a GAG similar to heparan sulfate. Heparitin Sulfate 244-259 fibroblast growth factor 2 Homo sapiens 29-32 33159882-7 2020 As heparan sulfate modulates FGF-mediated signaling, we found a significantly decreased activation of the MAP kinases ERK1/2 in FGF-2-stimulated cell lines of affected individuals that could be restored by addition of heparin, a GAG similar to heparan sulfate. Heparitin Sulfate 244-259 fibroblast growth factor 2 Homo sapiens 128-133 32656005-9 2020 Heparan sulfate (HS) normally acts as a storage site for basic fibroblast growth factor (bFGF), a paracrine stimulator of aldosterone, but accumulates in MPS-IIIA due to deficiency of heparan sulfatase. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 57-87 32656005-9 2020 Heparan sulfate (HS) normally acts as a storage site for basic fibroblast growth factor (bFGF), a paracrine stimulator of aldosterone, but accumulates in MPS-IIIA due to deficiency of heparan sulfatase. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 89-93 32656005-9 2020 Heparan sulfate (HS) normally acts as a storage site for basic fibroblast growth factor (bFGF), a paracrine stimulator of aldosterone, but accumulates in MPS-IIIA due to deficiency of heparan sulfatase. Heparitin Sulfate 17-19 fibroblast growth factor 2 Homo sapiens 57-87 32656005-9 2020 Heparan sulfate (HS) normally acts as a storage site for basic fibroblast growth factor (bFGF), a paracrine stimulator of aldosterone, but accumulates in MPS-IIIA due to deficiency of heparan sulfatase. Heparitin Sulfate 17-19 fibroblast growth factor 2 Homo sapiens 89-93 29458182-8 2018 Based upon mutually exclusive interactions of FGF2 with PI(4,5)P2 versus heparan sulfates, an assembly/disassembly pathway has been proposed to be the underlying principle of directional transport of FGF2 across the plasma membrane. Heparitin Sulfate 73-89 fibroblast growth factor 2 Homo sapiens 200-204 29458182-9 2018 Thus, the core mechanism of unconventional secretion of FGF2 is based upon three discrete steps with (i) PI(4,5)P2 dependent oligomerization of FGF2 at the inner leaflet, (ii) insertion of membrane spanning FGF2 oligomers into the plasma membrane and (iii) disassembly at the outer leaflet mediated by heparan sulfates that subsequently retain FGF2 on cell surfaces. Heparitin Sulfate 302-318 fibroblast growth factor 2 Homo sapiens 56-60 28864123-5 2018 In parallel, we also increased glucose concentration and supplied heparan sulfate to avoid depletion of glucose and bFGF, respectively. Heparitin Sulfate 66-81 fibroblast growth factor 2 Homo sapiens 116-120 28547849-4 2017 We previously found that fractones capture and store fibroblast growth factor 2 (FGF-2) via heparan sulfate binding, and may deliver FGF-2 to neural stem cells in a timely manner. Heparitin Sulfate 92-107 fibroblast growth factor 2 Homo sapiens 81-86 28722655-6 2017 Vectorial translocation of FGF2 across the membrane is governed by sequential and mutually exclusive interactions with PI(4,5)P2 and heparan sulfates on opposing sides of the membrane. Heparitin Sulfate 133-149 fibroblast growth factor 2 Homo sapiens 27-31 26543570-8 2015 PhRICS was then used to probe the diffusion speed of gold nanoparticle-labelled fibroblast growth factor 2 (FGF2) bound to heparan sulfate in the pericellular matrix of live fibroblast cells. Heparitin Sulfate 123-138 fibroblast growth factor 2 Homo sapiens 80-106 27291835-0 2017 Affinity Selection of FGF2-Binding Heparan Sulfates for Ex Vivo Expansion of Human Mesenchymal Stem Cells. Heparitin Sulfate 35-51 fibroblast growth factor 2 Homo sapiens 22-26 27291835-3 2017 The biological activity of FGF2 is controlled through interactions with heparan sulfate (HS) that facilitates ligand-receptor complex formation. Heparitin Sulfate 72-87 fibroblast growth factor 2 Homo sapiens 27-31 27291835-3 2017 The biological activity of FGF2 is controlled through interactions with heparan sulfate (HS) that facilitates ligand-receptor complex formation. Heparitin Sulfate 89-91 fibroblast growth factor 2 Homo sapiens 27-31 28078057-3 2016 Since heparanase is a kind of endo-beta-D-glucuronidase that is capable of cleaving heparan sulfate side chains of heparan sulfate proteoglycans on cell surfaces and the extracellular matrix, which further regulate the bioavailability of growth factors (FGF-2, TGF-beta). Heparitin Sulfate 84-99 fibroblast growth factor 2 Homo sapiens 254-259 27382052-3 2016 Key steps of this process are (i) phosphoinositide-dependent membrane recruitment, (ii) FGF2 oligomerization and membrane pore formation, and (iii) extracellular trapping mediated by membrane-proximal heparan sulfate proteoglycans. Heparitin Sulfate 201-216 fibroblast growth factor 2 Homo sapiens 88-92 26416892-6 2015 Hallmarks of unconventional secretion of FGF2 are: (i) sequential molecular interactions with the inner leaflet along with Tec kinase-dependent tyrosine phosphorylation of FGF2, (ii) PI(4,5)P2-dependent oligomerization and membrane pore formation, and (iii) extracellular trapping of FGF2 mediated by heparan sulfate proteoglycans on cell surfaces. Heparitin Sulfate 301-316 fibroblast growth factor 2 Homo sapiens 41-45 26079194-10 2015 However, FGF-2 was still captured by fractones via heparan sulfates. Heparitin Sulfate 51-67 fibroblast growth factor 2 Homo sapiens 9-14 33429571-1 2017 Fibroblast growth factor 2 (FGF-2), an important paracrine growth factor, binds electrostatically with low micromolar affinity to heparan sulfates present on extracellular matrix proteins. Heparitin Sulfate 130-146 fibroblast growth factor 2 Homo sapiens 0-26 33429571-1 2017 Fibroblast growth factor 2 (FGF-2), an important paracrine growth factor, binds electrostatically with low micromolar affinity to heparan sulfates present on extracellular matrix proteins. Heparitin Sulfate 130-146 fibroblast growth factor 2 Homo sapiens 28-33 33429571-2 2017 A single molecular analysis served as a basis to decipher the nanomechanical mechanism of the interaction between FGF-2 and the heparan sulfate surrogate, heparin, with a modular atomic force microscope (AFM) design combining magnetic actuators with force measurements at the low force regime (1 x 101 to 1 x 104 pN/s). Heparitin Sulfate 128-143 fibroblast growth factor 2 Homo sapiens 114-119 28249356-6 2017 Mislocalization of ss1-integrin, reductions in fibronectin, and alterations in heparan sulfate content all contribute to reduced FGF responsiveness in satellite cells. Heparitin Sulfate 79-94 fibroblast growth factor 2 Homo sapiens 129-132 27496348-0 2017 Dynamic changes in heparan sulfate during muscle differentiation and ageing regulate myoblast cell fate and FGF2 signalling. Heparitin Sulfate 19-34 fibroblast growth factor 2 Homo sapiens 108-112 28205415-0 2017 Microfluidic Screening Reveals Heparan Sulfate Enhances Human Mesenchymal Stem Cell Growth by Modulating Fibroblast Growth Factor-2 Transport. Heparitin Sulfate 31-46 fibroblast growth factor 2 Homo sapiens 105-131 28205415-3 2017 However, hMSCs also produce endogenous FGF-2, which critically interacts with cell surface heparan sulfate (HS). Heparitin Sulfate 91-106 fibroblast growth factor 2 Homo sapiens 39-44 28205415-3 2017 However, hMSCs also produce endogenous FGF-2, which critically interacts with cell surface heparan sulfate (HS). Heparitin Sulfate 108-110 fibroblast growth factor 2 Homo sapiens 39-44 28205415-4 2017 We assessed the interplay of FGF-2 with a heparan sulfate variant (HS8) engineered to bind FGF-2 and potentiate its activity. Heparitin Sulfate 42-57 fibroblast growth factor 2 Homo sapiens 91-96 27935954-3 2016 The formation of vessel sprouts, triggered by angiogenesis regulating factors with lowest affinities for heparan sulfate (e.g. VEGF), is followed by vessel-stabilizing PDGF-B or bFGF with medium affinity for HS, and by inhibitors such as PF-4 and TSP-1 with the highest affinities for HS. Heparitin Sulfate 105-120 fibroblast growth factor 2 Homo sapiens 178-182 26574650-6 2015 In an attempt to establish structure activity relationships of heparan sulfate binding with its receptor, the synthesized oligosaccharides were incorporated onto a glycan microarray, and their bindings with a growth factor FGF-2 were examined. Heparitin Sulfate 63-78 fibroblast growth factor 2 Homo sapiens 223-228 26336134-7 2015 The glycosylation of HRG may play a key competitive role in the interaction between HRG and heparin sulfate for binding bFGF and activating the FGF receptor. Heparitin Sulfate 92-107 fibroblast growth factor 2 Homo sapiens 120-124 26543570-8 2015 PhRICS was then used to probe the diffusion speed of gold nanoparticle-labelled fibroblast growth factor 2 (FGF2) bound to heparan sulfate in the pericellular matrix of live fibroblast cells. Heparitin Sulfate 123-138 fibroblast growth factor 2 Homo sapiens 108-112 25399576-5 2014 The majority of angiogenic cytokines, including VEGF-A, FGF2, IL-8 and SDF-1alpha, manifest an obligate dependence on heparan sulfate (HS) for their biological activity. Heparitin Sulfate 118-133 fibroblast growth factor 2 Homo sapiens 56-60 22815649-0 2012 Transport of fibroblast growth factor 2 in the pericellular matrix is controlled by the spatial distribution of its binding sites in heparan sulfate. Heparitin Sulfate 133-148 fibroblast growth factor 2 Homo sapiens 13-39 24509440-6 2014 Smooth muscle cell perlecan bound both FGF1 and FGF2 via its heparan sulfate chains and promoted the signaling of FGF2 but not FGF1. Heparitin Sulfate 61-76 fibroblast growth factor 2 Homo sapiens 48-52 24509440-7 2014 Also endothelial cell perlecan bound both FGF1 and FGF2 via its heparan sulfate chains, but in contrast, promoted the signaling of both growth factors. Heparitin Sulfate 64-79 fibroblast growth factor 2 Homo sapiens 51-55 23002246-3 2013 Here, we tested the hypothesis that matrix-associated heparan sulfate is enough to maintain hES cells under low fibroblast growth factor-2 concentration in the absence of live feeder cells. Heparitin Sulfate 54-69 fibroblast growth factor 2 Homo sapiens 112-138 23142801-5 2013 In this context, we investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus improve in vitro NSC cultivation. Heparitin Sulfate 60-75 fibroblast growth factor 2 Homo sapiens 214-240 23142801-5 2013 In this context, we investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus improve in vitro NSC cultivation. Heparitin Sulfate 60-75 fibroblast growth factor 2 Homo sapiens 242-247 23142801-5 2013 In this context, we investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus improve in vitro NSC cultivation. Heparitin Sulfate 77-79 fibroblast growth factor 2 Homo sapiens 214-240 23142801-5 2013 In this context, we investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus improve in vitro NSC cultivation. Heparitin Sulfate 77-79 fibroblast growth factor 2 Homo sapiens 242-247 23095131-3 2012 Heparanase-1, an endoglycosidase that cleaves heparan sulfate, is implicated in the pathogenesis of diabetic nephropathy and is necessary to FGF-2 for the induction of tubular cells transition. Heparitin Sulfate 46-61 fibroblast growth factor 2 Homo sapiens 141-146 24959968-0 2014 Interactions that influence the binding of synthetic heparan sulfate based disaccharides to fibroblast growth factor-2. Heparitin Sulfate 53-68 fibroblast growth factor 2 Homo sapiens 92-118 23510468-2 2013 We have previously shown that heparan sulphates (HS) associated with fractones bind fibroblast growth factor-2 (FGF-2), a powerful mitotic growth factor in the SVZa. Heparitin Sulfate 30-47 fibroblast growth factor 2 Homo sapiens 84-110 23510468-2 2013 We have previously shown that heparan sulphates (HS) associated with fractones bind fibroblast growth factor-2 (FGF-2), a powerful mitotic growth factor in the SVZa. Heparitin Sulfate 30-47 fibroblast growth factor 2 Homo sapiens 112-117 23510468-2 2013 We have previously shown that heparan sulphates (HS) associated with fractones bind fibroblast growth factor-2 (FGF-2), a powerful mitotic growth factor in the SVZa. Heparitin Sulfate 49-51 fibroblast growth factor 2 Homo sapiens 84-110 23510468-2 2013 We have previously shown that heparan sulphates (HS) associated with fractones bind fibroblast growth factor-2 (FGF-2), a powerful mitotic growth factor in the SVZa. Heparitin Sulfate 49-51 fibroblast growth factor 2 Homo sapiens 112-117 22927437-2 2012 The activity of these growth factors is regulated by heparan sulfate (HS), which is essential for the formation of FGF2/FGF receptor (FGFR) and VEGF(165)/VEGF receptor signaling complexes. Heparitin Sulfate 53-68 fibroblast growth factor 2 Homo sapiens 115-119 22927437-2 2012 The activity of these growth factors is regulated by heparan sulfate (HS), which is essential for the formation of FGF2/FGF receptor (FGFR) and VEGF(165)/VEGF receptor signaling complexes. Heparitin Sulfate 70-72 fibroblast growth factor 2 Homo sapiens 115-119 22815649-14 2012 We propose that this spatial organisation, coupled to the relative selectivity and the availability of HS-binding sites, determines the transport of FGF2 in matrices. Heparitin Sulfate 103-105 fibroblast growth factor 2 Homo sapiens 149-153 22102278-3 2012 Because the interaction between FGF-2 and its receptor is mediated by heparan sulfate (HS) and syndecans, we speculated that a deranged HS/syndecans regulation impairs FGF-2 activity. Heparitin Sulfate 70-85 fibroblast growth factor 2 Homo sapiens 32-37 22102278-3 2012 Because the interaction between FGF-2 and its receptor is mediated by heparan sulfate (HS) and syndecans, we speculated that a deranged HS/syndecans regulation impairs FGF-2 activity. Heparitin Sulfate 70-85 fibroblast growth factor 2 Homo sapiens 168-173 22102278-3 2012 Because the interaction between FGF-2 and its receptor is mediated by heparan sulfate (HS) and syndecans, we speculated that a deranged HS/syndecans regulation impairs FGF-2 activity. Heparitin Sulfate 87-89 fibroblast growth factor 2 Homo sapiens 32-37 22102278-3 2012 Because the interaction between FGF-2 and its receptor is mediated by heparan sulfate (HS) and syndecans, we speculated that a deranged HS/syndecans regulation impairs FGF-2 activity. Heparitin Sulfate 87-89 fibroblast growth factor 2 Homo sapiens 168-173 21572110-10 2011 The interaction of a hexamer of heparin sulfate was studied in interaction with fibroblast growth factor 2 and stromal cell-derived factor 1alpha. Heparitin Sulfate 32-47 fibroblast growth factor 2 Homo sapiens 80-106 21471211-0 2011 Highly sulfated nonreducing end-derived heparan sulfate domains bind fibroblast growth factor-2 with high affinity and are enriched in biologically active fractions. Heparitin Sulfate 40-55 fibroblast growth factor 2 Homo sapiens 69-95 21471211-3 2011 Effective binding of FGF2 to its receptor requires the presence of heparan sulfate (HS), a linear polysaccharide with N-sulfated domains (NS) localized at the cell surface and extracellular matrix. Heparitin Sulfate 67-82 fibroblast growth factor 2 Homo sapiens 21-25 21471211-3 2011 Effective binding of FGF2 to its receptor requires the presence of heparan sulfate (HS), a linear polysaccharide with N-sulfated domains (NS) localized at the cell surface and extracellular matrix. Heparitin Sulfate 84-86 fibroblast growth factor 2 Homo sapiens 21-25 21221614-4 2011 The degradation of heparan sulfate resulted in a marked reduction of binding activity of the basement membrane for vascular endothelial growth factor, fibroblast growth factor-2 and -7 at the dermal-epidermal junction. Heparitin Sulfate 19-34 fibroblast growth factor 2 Homo sapiens 151-184 21112773-7 2011 GPC3 is also able to bind basic growth factors such as fibroblast growth factor 2 through its heparan sulphate glycan chains. Heparitin Sulfate 94-110 fibroblast growth factor 2 Homo sapiens 55-81 21447157-3 2011 Beside the well-known stabilization of FGF2 by heparin or heparan sulphate, the recently discovered binding to ATP also shows a stabilizing and protective effect on this growth factor. Heparitin Sulfate 58-74 fibroblast growth factor 2 Homo sapiens 39-43 20865198-3 2010 Differential scanning fluorimetry was used to measure the thermostabilisation bestowed by modified heparin polysaccharides (proxies for heparan sulfate) on fibroblast growth factor-1 (FGF-1) and fibroblast growth factor-2 (FGF-2), prototypical heparan sulfate-binding proteins, revealing varied abilities and primary sequence-activity redundancy. Heparitin Sulfate 136-151 fibroblast growth factor 2 Homo sapiens 195-221 20678572-1 2010 Fibroblast growth factor-2 (FGF2) is produced and released by endothelial cells and binds to heparan sulfate proteoglycans in the endothelial basement membrane (BM), an important FGF2 storage reservoir. Heparitin Sulfate 93-108 fibroblast growth factor 2 Homo sapiens 0-26 20678572-1 2010 Fibroblast growth factor-2 (FGF2) is produced and released by endothelial cells and binds to heparan sulfate proteoglycans in the endothelial basement membrane (BM), an important FGF2 storage reservoir. Heparitin Sulfate 93-108 fibroblast growth factor 2 Homo sapiens 28-32 20230531-2 2010 Unconventional secretion of FGF2 occurs by direct translocation across plasma membranes, a process that depends on the phosphoinositide phosphatidylinositol 4,5-biphosphate (PI(4,5)P(2)) at the inner leaflet as well as heparan sulfate proteoglycans at the outer leaflet of plasma membranes; however, additional core and regulatory components of the FGF2 export machinery have remained elusive. Heparitin Sulfate 219-234 fibroblast growth factor 2 Homo sapiens 28-32 20564189-0 2010 Controlled delivery of the heparan sulfate/FGF-2 complex by a polyelectrolyte scaffold promotes maximal hMSC proliferation and differentiation. Heparitin Sulfate 27-42 fibroblast growth factor 2 Homo sapiens 43-48 20564189-3 2010 The aim of this study was to investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus enhance hMSC stimulation. Heparitin Sulfate 69-84 fibroblast growth factor 2 Homo sapiens 223-249 20564189-3 2010 The aim of this study was to investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus enhance hMSC stimulation. Heparitin Sulfate 69-84 fibroblast growth factor 2 Homo sapiens 251-256 20564189-3 2010 The aim of this study was to investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus enhance hMSC stimulation. Heparitin Sulfate 86-88 fibroblast growth factor 2 Homo sapiens 223-249 20564189-3 2010 The aim of this study was to investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus enhance hMSC stimulation. Heparitin Sulfate 86-88 fibroblast growth factor 2 Homo sapiens 251-256 19942287-2 2010 In vivo, fibroblast growth factor 2 (FGF-2) binds to the sulfated glycosaminoglycan heparan sulfate, which is a major component of the ECM. Heparitin Sulfate 84-99 fibroblast growth factor 2 Homo sapiens 9-35 19942287-2 2010 In vivo, fibroblast growth factor 2 (FGF-2) binds to the sulfated glycosaminoglycan heparan sulfate, which is a major component of the ECM. Heparitin Sulfate 84-99 fibroblast growth factor 2 Homo sapiens 37-42 19259985-1 2009 Heparan sulfate proteoglycans cooperate with basic fibroblast growth factor (bFGF/FGF2) signaling to control osteoblast growth and differentiation, as well as metabolic functions of osteoblasts. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 77-81 19788922-7 2010 In contrast, the heparan sulfate chains of HOCl/HOBr-modified perlecan retained their ability to bind FGF-2 and collagen V and were able to promote FGF-2-dependent cellular proliferation. Heparitin Sulfate 17-32 fibroblast growth factor 2 Homo sapiens 102-107 19788922-7 2010 In contrast, the heparan sulfate chains of HOCl/HOBr-modified perlecan retained their ability to bind FGF-2 and collagen V and were able to promote FGF-2-dependent cellular proliferation. Heparitin Sulfate 17-32 fibroblast growth factor 2 Homo sapiens 148-153 19110070-4 2009 Here, treatment with FGF-2 strongly reduces the expression levels of the heparan sulfate-containing proteoglycan, syndecan-4. Heparitin Sulfate 73-88 fibroblast growth factor 2 Homo sapiens 21-26 19259985-1 2009 Heparan sulfate proteoglycans cooperate with basic fibroblast growth factor (bFGF/FGF2) signaling to control osteoblast growth and differentiation, as well as metabolic functions of osteoblasts. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 82-86 18459126-4 2008 Moreover, our data suggested that PGF2alpha could induce new synthesis of heparan sulphate (HS) chains on osteoblasts by a mechanism involving a modulation of MAPK signalling and that HS is required for the regulation of FGF-2 induced by PGF2alpha. Heparitin Sulfate 92-94 fibroblast growth factor 2 Homo sapiens 221-226 19320882-3 2009 The ECM can directly bind to and release certain growth factors (e.g., heparan sulfate binding to fibroblast growth factor-2), which may serve to sequester and protect growth factors from degradation, and/or enhance their activity. Heparitin Sulfate 71-86 fibroblast growth factor 2 Homo sapiens 98-124 17706452-8 2008 Both the amounts of chondroitin/dermatan/heparan sulfate and their sulfation levels differed between the cell lines and were distinctly modulated by FGF-2. Heparitin Sulfate 41-56 fibroblast growth factor 2 Homo sapiens 149-154 18725627-5 2008 Addition of surfen to cultured cells blocked FGF2-binding and signaling that depended on cell surface heparan sulfate and prevented both FGF2- and VEGF(165)-mediated sprouting of endothelial cells in Matrigel. Heparitin Sulfate 102-117 fibroblast growth factor 2 Homo sapiens 45-49 17706452-9 2008 In this study, we show that chondroitin/dermatan sulfate-containing proteoglycans, likely in cooperation with heparan sulfate, participate in metastatic melanoma cell FGF-2-induced mitogenic response, which represents a novel finding and establishes the central role of sulfated glycosaminoglycans on melanoma growth. Heparitin Sulfate 110-125 fibroblast growth factor 2 Homo sapiens 167-172 16778174-6 2006 More importantly, HSulf-1 expression in the xenografts was associated with a reduced ability of vascular endothelial cell heparan sulfate to participate in a complex with fibroblast growth factor 2 (FGF-2) and its receptor tyrosine kinase FGF receptor 1c. Heparitin Sulfate 122-137 fibroblast growth factor 2 Homo sapiens 199-204 16829530-1 2006 The regulation of cell function by fibroblast growth factors (FGF) occurs through a dual receptor system consisting of a receptor-tyrosine kinase, FGFR and the glycosaminoglycan heparan sulfate (HS). Heparitin Sulfate 178-193 fibroblast growth factor 2 Homo sapiens 62-65 16829530-1 2006 The regulation of cell function by fibroblast growth factors (FGF) occurs through a dual receptor system consisting of a receptor-tyrosine kinase, FGFR and the glycosaminoglycan heparan sulfate (HS). Heparitin Sulfate 195-197 fibroblast growth factor 2 Homo sapiens 62-65 16823965-5 2006 The specificity of the receptor-ligand interaction was confirmed by the lack of cell binding to the negative control proteins, cytochrome c and insulin, and by the disruption of cell binding by treatment with heparitinase to destroy heparan sulfate which plays an essential role in the binding of bFGF to FGF receptors. Heparitin Sulfate 233-248 fibroblast growth factor 2 Homo sapiens 297-301 16823965-5 2006 The specificity of the receptor-ligand interaction was confirmed by the lack of cell binding to the negative control proteins, cytochrome c and insulin, and by the disruption of cell binding by treatment with heparitinase to destroy heparan sulfate which plays an essential role in the binding of bFGF to FGF receptors. Heparitin Sulfate 233-248 fibroblast growth factor 2 Homo sapiens 298-301 16778174-6 2006 More importantly, HSulf-1 expression in the xenografts was associated with a reduced ability of vascular endothelial cell heparan sulfate to participate in a complex with fibroblast growth factor 2 (FGF-2) and its receptor tyrosine kinase FGF receptor 1c. Heparitin Sulfate 122-137 fibroblast growth factor 2 Homo sapiens 171-197 17368052-8 2007 RESULTS: Here we have identified perlecan as the heparan sulphate proteoglycan that sequesters FGF-2 in articular cartilage. Heparitin Sulfate 49-65 fibroblast growth factor 2 Homo sapiens 95-100 17437011-1 2007 Several angiogenic growth factors including fibroblast growth factors 1 and 2 (FGF1 and FGF2) depend on heparan sulphate (HS) for biological activity. Heparitin Sulfate 104-120 fibroblast growth factor 2 Homo sapiens 88-92 17437011-1 2007 Several angiogenic growth factors including fibroblast growth factors 1 and 2 (FGF1 and FGF2) depend on heparan sulphate (HS) for biological activity. Heparitin Sulfate 122-124 fibroblast growth factor 2 Homo sapiens 88-92 17326680-5 2007 Fibers were coated, after processing, with perlecan domain I (PlnDI) to improve binding of basic fibroblast growth factor (FGF-2), which binds to native heparan sulfate chains on PlnDI. Heparitin Sulfate 153-168 fibroblast growth factor 2 Homo sapiens 123-128 16951194-6 2006 In addition, JG3 abolished heparanase-driven invasion, inhibited the release of heparan sulfate-sequestered basic fibroblast growth factor (bFGF) from the extracellular matrix, and repressed subsequent angiogenesis. Heparitin Sulfate 80-95 fibroblast growth factor 2 Homo sapiens 108-138 16951194-6 2006 In addition, JG3 abolished heparanase-driven invasion, inhibited the release of heparan sulfate-sequestered basic fibroblast growth factor (bFGF) from the extracellular matrix, and repressed subsequent angiogenesis. Heparitin Sulfate 80-95 fibroblast growth factor 2 Homo sapiens 140-144 16778174-7 2006 In vitro, short hairpin RNA-mediated down-regulation of HSulf-1 in human umbilical vein endothelial cells (HUVEC) resulted in an increased proliferation mediated by heparan sulfate-dependent FGF-2, hepatocyte growth factor, and vascular endothelial growth factor 165 (VEGF165) but not by heparan sulfate-independent VEGF121. Heparitin Sulfate 165-180 fibroblast growth factor 2 Homo sapiens 191-196 16288042-0 2005 Laminin alpha5 chain metastasis- and angiogenesis-inhibiting peptide blocks fibroblast growth factor 2 activity by binding to the heparan sulfate chains of CD44. Heparitin Sulfate 130-145 fibroblast growth factor 2 Homo sapiens 76-102 16260789-4 2005 By selecting appropriate enzymatic modification steps, an inactive precursor has been converted to the heparan sulfate having three distinct biological activities, associated with binding to antithrombin, fibroblast growth factor-2, and herpes simplex virus envelope glycoprotein D. Because the recombinant sulfotransferases are expressed in bacteria, and the method uses a low cost sulfo donor, it can be readily utilized to synthesize large quantities of anticoagulant heparin drug or other biologically active heparan sulfates. Heparitin Sulfate 103-118 fibroblast growth factor 2 Homo sapiens 205-231 16192265-2 2005 Recent studies show that when the levels of 6-O-sulfation of heparan sulfate are diminished by the activity of extracellular heparan sulfate 6-O-endosulfatases (Sulfs), fibroblast growth factor 2-, heparin binding epidermal growth factor-, and hepatocyte growth factor-mediated signaling are attenuated. Heparitin Sulfate 61-76 fibroblast growth factor 2 Homo sapiens 169-195 16192265-2 2005 Recent studies show that when the levels of 6-O-sulfation of heparan sulfate are diminished by the activity of extracellular heparan sulfate 6-O-endosulfatases (Sulfs), fibroblast growth factor 2-, heparin binding epidermal growth factor-, and hepatocyte growth factor-mediated signaling are attenuated. Heparitin Sulfate 125-140 fibroblast growth factor 2 Homo sapiens 169-195 16288042-8 2005 We conclude that peptide A5G27 inhibits metastasis and angiogenesis by blocking FGF2 binding to the heparan sulfate side chains of CD44 variant 3, thus decreasing FGF2 bioactivity. Heparitin Sulfate 100-115 fibroblast growth factor 2 Homo sapiens 80-84 15947088-0 2005 Functional abnormalities of heparan sulfate in mucopolysaccharidosis-I are associated with defective biologic activity of FGF-2 on human multipotent progenitor cells. Heparitin Sulfate 28-43 fibroblast growth factor 2 Homo sapiens 122-127 15958243-2 2005 The covalently bound heparin provided a crosslinkable analog of a heparan sulfate proteoglycan, thus providing a multivalent biomaterial capable of controlled release of basic fibroblast growth factor (bFGF). Heparitin Sulfate 66-81 fibroblast growth factor 2 Homo sapiens 170-200 15958243-2 2005 The covalently bound heparin provided a crosslinkable analog of a heparan sulfate proteoglycan, thus providing a multivalent biomaterial capable of controlled release of basic fibroblast growth factor (bFGF). Heparitin Sulfate 66-81 fibroblast growth factor 2 Homo sapiens 202-206 16175541-7 2005 Monte Carlo multiple-minima calculations of low-energy conformers of the FGF-bound ligands showed that all of the sulfated tetracyclitol ligands can bind effectively in the heparan sulfate-binding sites of FGF-1 and FGF-2. Heparitin Sulfate 173-188 fibroblast growth factor 2 Homo sapiens 216-221 17216117-4 2005 Normal cornea contains heparan sulfate to prevent the release of potent angiogenic cytokines, such as basic fibroblast growth factor (bFGF) from the Bowman"s layer. Heparitin Sulfate 23-38 fibroblast growth factor 2 Homo sapiens 102-132 17216117-4 2005 Normal cornea contains heparan sulfate to prevent the release of potent angiogenic cytokines, such as basic fibroblast growth factor (bFGF) from the Bowman"s layer. Heparitin Sulfate 23-38 fibroblast growth factor 2 Homo sapiens 134-138 15334457-9 2004 Up-regulation of RANKL by FGF-2 on RASFs was diminished by the removal of heparan sulfate with heparitinase. Heparitin Sulfate 74-89 fibroblast growth factor 2 Homo sapiens 26-31 15738663-3 2005 Heparan sulfate chains bind and potentiate the activities of various growth factors such as fibroblast growth factor 2 (FGF-2). Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 92-118 15738663-3 2005 Heparan sulfate chains bind and potentiate the activities of various growth factors such as fibroblast growth factor 2 (FGF-2). Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 120-125 15738663-10 2005 Heparitinase digestion demonstrated that recombinant PlnDI was substituted with heparan sulfate and that these heparan sulfate chains were critically important not only for efficient integration of PlnDI into scaffolds, but also for FGF-2 binding and retention. Heparitin Sulfate 111-126 fibroblast growth factor 2 Homo sapiens 233-238 15958608-1 2005 Fibroblast growth factor-2 (FGF-2) is a potent angiogenic cytokine that is dependent on heparan sulfate for its biological activity. Heparitin Sulfate 88-103 fibroblast growth factor 2 Homo sapiens 0-26 15958608-1 2005 Fibroblast growth factor-2 (FGF-2) is a potent angiogenic cytokine that is dependent on heparan sulfate for its biological activity. Heparitin Sulfate 88-103 fibroblast growth factor 2 Homo sapiens 28-33 15958608-3 2005 Using a unique molecular probe, FR1c-Ap, which consisted of a soluble FGF receptor 1 isoform IIIc covalently linked to an alkaline phosphatase moiety, the distribution of heparan sulfate that had the ability to support the formation of a heparan sulfate/FGF-2/FGFR1 isoform IIIc alkaline phosphatase heparan sulfate construct complex was determined. Heparitin Sulfate 171-186 fibroblast growth factor 2 Homo sapiens 254-259 15958608-7 2005 Double-staining techniques showed that heparan sulfate was found predominantly at the basal aspect of the endothelium and suggested that syndecan-3 might function as one of the proteoglycans involved in FGF-2 signaling in the endothelium. Heparitin Sulfate 39-54 fibroblast growth factor 2 Homo sapiens 203-208 15548653-2 2004 Here, we report a functional interaction between anosmin-1 and the FGFR1-FGF2-heparan sulfate complex, leading to amplified responses in the FGFR1 signaling pathway. Heparitin Sulfate 78-93 fibroblast growth factor 2 Homo sapiens 73-77 15260846-1 2004 Heparan sulfate (HS), which is degraded by heparanase, plays an important role in cell adhesion, insolubility of the extracellular matrix (ECM) and as a reservoir for various growth factors such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 198-228 15260846-1 2004 Heparan sulfate (HS), which is degraded by heparanase, plays an important role in cell adhesion, insolubility of the extracellular matrix (ECM) and as a reservoir for various growth factors such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 230-234 15260846-1 2004 Heparan sulfate (HS), which is degraded by heparanase, plays an important role in cell adhesion, insolubility of the extracellular matrix (ECM) and as a reservoir for various growth factors such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). Heparitin Sulfate 17-19 fibroblast growth factor 2 Homo sapiens 198-228 15260846-1 2004 Heparan sulfate (HS), which is degraded by heparanase, plays an important role in cell adhesion, insolubility of the extracellular matrix (ECM) and as a reservoir for various growth factors such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). Heparitin Sulfate 17-19 fibroblast growth factor 2 Homo sapiens 230-234 15223860-0 2004 Epithelial expression and release of FGF-2 from heparan sulphate binding sites in bronchial tissue in asthma. Heparitin Sulfate 48-64 fibroblast growth factor 2 Homo sapiens 37-42 14707131-9 2004 Octasaccharides with the high affinity to FGF-2 and FGF-10 had the activity to release them, respectively, from their complexes with HS. Heparitin Sulfate 133-135 fibroblast growth factor 2 Homo sapiens 42-47 15081013-3 2004 The affinities of these heparan sulfate (HS) mimetics for the HS-binding fibroblast growth factors FGF-1 and FGF-2 were measured via a surface plasmon resonance solution affinity assay. Heparitin Sulfate 24-39 fibroblast growth factor 2 Homo sapiens 109-114 15081013-3 2004 The affinities of these heparan sulfate (HS) mimetics for the HS-binding fibroblast growth factors FGF-1 and FGF-2 were measured via a surface plasmon resonance solution affinity assay. Heparitin Sulfate 41-43 fibroblast growth factor 2 Homo sapiens 109-114 14684627-6 2004 The FGF2 activation of ERK1/2 in the absence of receptor activity was completely dependent on heparan sulfate, because this activity was abolished by heparinase III digestion of the cells. Heparitin Sulfate 94-109 fibroblast growth factor 2 Homo sapiens 4-8 15702865-1 2004 Fibroblast growth factor-2 is a member of a large family of proteins that bind heparin and heparan sulfate and modulate the function of a wide range of cell types. Heparitin Sulfate 91-106 fibroblast growth factor 2 Homo sapiens 0-26 14733594-2 2004 We demonstrate that a glycopolymer bearing pendant, fully sulfated lactose units effectively replaces heparin and heparan sulfate as a molecular chaperone for fibroblast growth factor-2 (FGF-2). Heparitin Sulfate 114-129 fibroblast growth factor 2 Homo sapiens 159-185 14733594-2 2004 We demonstrate that a glycopolymer bearing pendant, fully sulfated lactose units effectively replaces heparin and heparan sulfate as a molecular chaperone for fibroblast growth factor-2 (FGF-2). Heparitin Sulfate 114-129 fibroblast growth factor 2 Homo sapiens 187-192 12672474-0 2003 Interaction of platelet factor 4 with fibroblast growth factor 2 is stabilised by heparan sulphate. Heparitin Sulfate 82-98 fibroblast growth factor 2 Homo sapiens 38-64 15035435-8 2004 Heparin and heparan sulfate fully protected bFGF from complexation and cleavage by HABP, although these glycosaminoglycans are known to enhance the proteolytic activity of HABP. Heparitin Sulfate 12-27 fibroblast growth factor 2 Homo sapiens 44-48 12672474-2 2003 We investigated the heparan sulphate-dependent mechanism of PF4 inhibition of fibroblast growth factor 2 (FGF-2). Heparitin Sulfate 20-36 fibroblast growth factor 2 Homo sapiens 78-104 12672474-2 2003 We investigated the heparan sulphate-dependent mechanism of PF4 inhibition of fibroblast growth factor 2 (FGF-2). Heparitin Sulfate 20-36 fibroblast growth factor 2 Homo sapiens 106-111 12598330-4 2003 A response was acquired, however, when myofibroblasts were incubated with FGF-2 in the presence of heparan sulfate (HS) and heparin. Heparitin Sulfate 99-114 fibroblast growth factor 2 Homo sapiens 74-79 12761845-9 2003 We find that FGF2 stimulation of proliferation is inhibited in the presence of sodium chlorate, an inhibitor of heparan sulfate synthesis, and is rescued by addition of exogenous heparan sulfate. Heparitin Sulfate 112-127 fibroblast growth factor 2 Homo sapiens 13-17 12761845-9 2003 We find that FGF2 stimulation of proliferation is inhibited in the presence of sodium chlorate, an inhibitor of heparan sulfate synthesis, and is rescued by addition of exogenous heparan sulfate. Heparitin Sulfate 179-194 fibroblast growth factor 2 Homo sapiens 13-17 12761845-10 2003 These data support a requirement for heparan sulfate in FGF signalling for proliferation of brain precursor cells. Heparitin Sulfate 37-52 fibroblast growth factor 2 Homo sapiens 56-59 12697740-3 2003 CD44-negative Namalwa cells transfected with CD44vRA cDNA or with CD44v3-v10 (CD44vRA wild type) cDNA bound FGF-2 to an equal extent via their associated heparan sulfate chains. Heparitin Sulfate 154-169 fibroblast growth factor 2 Homo sapiens 108-113 12697740-8 2003 Hence, FGF-2 attached to the heparan sulfate moiety expressed by the novel CD44 variant of RA synovium cells exhibits an augmented ability to stimulate FGFR-1-mediated activities. Heparitin Sulfate 29-44 fibroblast growth factor 2 Homo sapiens 7-12 12591930-5 2003 Here we show that all brain endothelial cell HSPGs carry heparan sulfate chains similarly capable of forming a ternary complex with FGF2 and fibroblast growth factor receptor-1c and of promoting a mitogenic signal. Heparitin Sulfate 57-72 fibroblast growth factor 2 Homo sapiens 132-136 12598330-4 2003 A response was acquired, however, when myofibroblasts were incubated with FGF-2 in the presence of heparan sulfate (HS) and heparin. Heparitin Sulfate 116-118 fibroblast growth factor 2 Homo sapiens 74-79 12583005-2 2003 Although the structural requirements of heparin and heparan sulfate for the high-affinity binding to bFGF have been extensively examined, studies on intact heparin proteoglycans are limited. Heparitin Sulfate 52-67 fibroblast growth factor 2 Homo sapiens 101-105 12034712-2 2002 Heparan sulfate (HS) regulates the kinetics of fibroblast growth factor 2 (FGF2)-stimulated intracellular signaling and differentially activates cell proliferation of cells expressing different FGF receptors (FGFRs). Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 47-73 12656349-0 2003 Surface plasmon resonance analysis to evaluate the importance of heparin sulfate groups" binding with human aFGF and bFGF. Heparitin Sulfate 65-80 fibroblast growth factor 2 Homo sapiens 117-121 12440867-3 2002 Mono- and disaccharide-containing glycopolymers were synthesized by two different free-radical processes, and their ability to act as heparan sulfate glycomimetics in promoting the binding of Fibroblast Growth Factor-2 (FGF-2) to its receptor (FGFR-1) was evaluated using an in vitro cell-based assay. Heparitin Sulfate 134-149 fibroblast growth factor 2 Homo sapiens 192-218 12440867-3 2002 Mono- and disaccharide-containing glycopolymers were synthesized by two different free-radical processes, and their ability to act as heparan sulfate glycomimetics in promoting the binding of Fibroblast Growth Factor-2 (FGF-2) to its receptor (FGFR-1) was evaluated using an in vitro cell-based assay. Heparitin Sulfate 134-149 fibroblast growth factor 2 Homo sapiens 220-225 12034712-2 2002 Heparan sulfate (HS) regulates the kinetics of fibroblast growth factor 2 (FGF2)-stimulated intracellular signaling and differentially activates cell proliferation of cells expressing different FGF receptors (FGFRs). Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 75-79 12034712-2 2002 Heparan sulfate (HS) regulates the kinetics of fibroblast growth factor 2 (FGF2)-stimulated intracellular signaling and differentially activates cell proliferation of cells expressing different FGF receptors (FGFRs). Heparitin Sulfate 17-19 fibroblast growth factor 2 Homo sapiens 47-73 12034712-2 2002 Heparan sulfate (HS) regulates the kinetics of fibroblast growth factor 2 (FGF2)-stimulated intracellular signaling and differentially activates cell proliferation of cells expressing different FGF receptors (FGFRs). Heparitin Sulfate 17-19 fibroblast growth factor 2 Homo sapiens 75-79 11956326-0 2002 Increased responsiveness of hypoxic endothelial cells to FGF2 is mediated by HIF-1alpha-dependent regulation of enzymes involved in synthesis of heparan sulfate FGF2-binding sites. Heparitin Sulfate 145-160 fibroblast growth factor 2 Homo sapiens 57-61 11889131-1 2002 Syndecan-4 is a heparan sulfate-carrying core protein that has been directly implicated in fibroblast growth factor 2 (FGF2) signaling. Heparitin Sulfate 16-31 fibroblast growth factor 2 Homo sapiens 91-117 11889131-1 2002 Syndecan-4 is a heparan sulfate-carrying core protein that has been directly implicated in fibroblast growth factor 2 (FGF2) signaling. Heparitin Sulfate 16-31 fibroblast growth factor 2 Homo sapiens 119-123 11956326-0 2002 Increased responsiveness of hypoxic endothelial cells to FGF2 is mediated by HIF-1alpha-dependent regulation of enzymes involved in synthesis of heparan sulfate FGF2-binding sites. Heparitin Sulfate 145-160 fibroblast growth factor 2 Homo sapiens 161-165 11956326-1 2002 Binding of basic fibroblast growth factor (FGF2) to its high affinity receptors requires the presence of specific heparan sulfate (HS) moieties on the cell surface that act as coreceptors. Heparitin Sulfate 114-129 fibroblast growth factor 2 Homo sapiens 43-47 11956326-1 2002 Binding of basic fibroblast growth factor (FGF2) to its high affinity receptors requires the presence of specific heparan sulfate (HS) moieties on the cell surface that act as coreceptors. Heparitin Sulfate 131-133 fibroblast growth factor 2 Homo sapiens 43-47 11847221-1 2002 Human basement membrane heparan sulfate proteoglycan (HSPG) perlecan binds and activates fibroblast growth factor (FGF)-2 through its heparan sulfate (HS) chains. Heparitin Sulfate 24-39 fibroblast growth factor 2 Homo sapiens 89-121 11847221-1 2002 Human basement membrane heparan sulfate proteoglycan (HSPG) perlecan binds and activates fibroblast growth factor (FGF)-2 through its heparan sulfate (HS) chains. Heparitin Sulfate 54-56 fibroblast growth factor 2 Homo sapiens 89-121 12097308-0 2002 Heparan sulfate interacting protein (HIP/L29) negatively regulates growth responses to basic fibroblast growth factor in gingival fibroblasts. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 87-117 11509569-5 2001 We show that recombinant FGF-BP1 specifically binds FGF-2 and that this binding is inhibited by FGF-1, heparan sulfate, and heparinoids. Heparitin Sulfate 103-118 fibroblast growth factor 2 Homo sapiens 52-57 11724555-0 2001 Structural basis for interaction of FGF-1, FGF-2, and FGF-7 with different heparan sulfate motifs. Heparitin Sulfate 75-90 fibroblast growth factor 2 Homo sapiens 43-48 11551944-0 2001 Membrane heparan sulfate proteoglycan-supported FGF2-FGFR1 signaling: evidence in support of the "cooperative end structures" model. Heparitin Sulfate 9-24 fibroblast growth factor 2 Homo sapiens 48-52 11551944-1 2001 Fibroblast growth factor 2 (FGF2)-initiated FGF receptor (FGFR)-signaling requires the assistance of heparin/heparan sulfate. Heparitin Sulfate 109-124 fibroblast growth factor 2 Homo sapiens 0-26 11551944-1 2001 Fibroblast growth factor 2 (FGF2)-initiated FGF receptor (FGFR)-signaling requires the assistance of heparin/heparan sulfate. Heparitin Sulfate 109-124 fibroblast growth factor 2 Homo sapiens 28-32 12463507-8 2002 FGF2 bound specifically to surface-adsorbed fibrinogen, fibronectin and vitronectin as well as to pre-coated heparan sulphate proteoglycan, perlecan. Heparitin Sulfate 109-125 fibroblast growth factor 2 Homo sapiens 0-4 11679141-5 2001 These heparan sulfates exhibited a predominant inhibitory effect on fibroblast growth factor-2 binding to fibroblast growth factor receptor-1, when tested in cells deficient in cell surface heparan sulfates. Heparitin Sulfate 6-22 fibroblast growth factor 2 Homo sapiens 68-94 11406624-2 2001 Several FGFs, including the prototype factors FGF-1 and FGF-2, depend on interactions with heparan sulfate (HS) proteoglycans for activity. Heparitin Sulfate 91-106 fibroblast growth factor 2 Homo sapiens 56-61 11406624-2 2001 Several FGFs, including the prototype factors FGF-1 and FGF-2, depend on interactions with heparan sulfate (HS) proteoglycans for activity. Heparitin Sulfate 108-110 fibroblast growth factor 2 Homo sapiens 56-61 11292822-2 2001 In the case of fibroblast growth factor-2 (FGF2) signaling, heparin/heparan sulfate-like glycosaminoglycans (HLGAGs) are involved through interaction with both FGF2 and its receptors (FGFRs) in assembling a tertiary complex and modulating FGF2 activity. Heparitin Sulfate 68-83 fibroblast growth factor 2 Homo sapiens 15-41 11292822-2 2001 In the case of fibroblast growth factor-2 (FGF2) signaling, heparin/heparan sulfate-like glycosaminoglycans (HLGAGs) are involved through interaction with both FGF2 and its receptors (FGFRs) in assembling a tertiary complex and modulating FGF2 activity. Heparitin Sulfate 68-83 fibroblast growth factor 2 Homo sapiens 43-47 11292822-2 2001 In the case of fibroblast growth factor-2 (FGF2) signaling, heparin/heparan sulfate-like glycosaminoglycans (HLGAGs) are involved through interaction with both FGF2 and its receptors (FGFRs) in assembling a tertiary complex and modulating FGF2 activity. Heparitin Sulfate 68-83 fibroblast growth factor 2 Homo sapiens 160-164 11292822-2 2001 In the case of fibroblast growth factor-2 (FGF2) signaling, heparin/heparan sulfate-like glycosaminoglycans (HLGAGs) are involved through interaction with both FGF2 and its receptors (FGFRs) in assembling a tertiary complex and modulating FGF2 activity. Heparitin Sulfate 68-83 fibroblast growth factor 2 Homo sapiens 160-164 11679141-6 2001 During wound healing, there was a marked decrease in the relative inhibitory activity of the extracted heparan sulfates on fibroblast growth factor-2-receptor binding. Heparitin Sulfate 103-119 fibroblast growth factor 2 Homo sapiens 123-149 11215206-3 2000 The biological functions of this enzyme in vivo were as follows: 1) this enzyme accelerates cancer cell invasion and metastasis though the degradation of vascular basement membrane and extracellular matrix by cancer cells; 2) this enzyme releases and activates heparin-binding growth factors such as bFGF and VEGF from heparan sulfate proteoglycans, and induces angiogenesis; 3) the degradative products of heparan sulfate proteoglycans by this enzyme suppress the biological function of activated T-lymphocytes. Heparitin Sulfate 319-334 fibroblast growth factor 2 Homo sapiens 300-304 11380810-3 2001 Furthermore, in many cells, the activity of FGF-2 is controlled by a low-affinity but high-capacity interaction with heparan sulfate (HS) proteoglycans (PGs), such as members of the syndecan family. Heparitin Sulfate 117-132 fibroblast growth factor 2 Homo sapiens 44-49 11380810-3 2001 Furthermore, in many cells, the activity of FGF-2 is controlled by a low-affinity but high-capacity interaction with heparan sulfate (HS) proteoglycans (PGs), such as members of the syndecan family. Heparitin Sulfate 134-136 fibroblast growth factor 2 Homo sapiens 44-49 11380810-11 2001 The addition of conditioned medium, containing HS-GAG fragments, restored the proliferative response to FGF-2, confirming the specificity of the interaction. Heparitin Sulfate 47-49 fibroblast growth factor 2 Homo sapiens 104-109 10881022-12 2000 FGF-2 can bind to heparin sulfate chains within the extracellular matrix (ECM). Heparitin Sulfate 18-33 fibroblast growth factor 2 Homo sapiens 0-5 11081250-9 2000 Heparan sulfate-containing fractions also showed FGF-2 immunoreactivity, indicating the complex formation of FGF-2 and heparan sulfate. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 49-54 11081250-9 2000 Heparan sulfate-containing fractions also showed FGF-2 immunoreactivity, indicating the complex formation of FGF-2 and heparan sulfate. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 109-114 11081250-9 2000 Heparan sulfate-containing fractions also showed FGF-2 immunoreactivity, indicating the complex formation of FGF-2 and heparan sulfate. Heparitin Sulfate 119-134 fibroblast growth factor 2 Homo sapiens 49-54 11081250-12 2000 This loss of a subpopulation of FGF-2-binding heparan sulfate in human papillary thyroid carcinomas may lead to the increase of free FGF-2 bioavailable in extracellular matrix. Heparitin Sulfate 46-61 fibroblast growth factor 2 Homo sapiens 32-37 11081250-12 2000 This loss of a subpopulation of FGF-2-binding heparan sulfate in human papillary thyroid carcinomas may lead to the increase of free FGF-2 bioavailable in extracellular matrix. Heparitin Sulfate 46-61 fibroblast growth factor 2 Homo sapiens 133-138 11081250-2 2000 In order to test this hypothesis, we established a simple and sensitive method for detecting FGF-2-binding heparan sulfates and characterized them in papillary thyroid carcinomas and normal thyroids. Heparitin Sulfate 107-123 fibroblast growth factor 2 Homo sapiens 93-98 10838166-7 2000 These results suggest that OSF is a highly sulfated unique polysaccharide that can promote the binding of bFGF to the heparan sulfate molecules required for binding to the high affinity receptors with tyrosine kinase activity. Heparitin Sulfate 118-133 fibroblast growth factor 2 Homo sapiens 106-110 10687947-1 2000 Fibroblast growth factor-2 (FGF-2) is a member of a large family of proteins that bind heparin and heparan sulfate and modulate the function of a wide range of cell types. Heparitin Sulfate 99-114 fibroblast growth factor 2 Homo sapiens 0-26 10687947-1 2000 Fibroblast growth factor-2 (FGF-2) is a member of a large family of proteins that bind heparin and heparan sulfate and modulate the function of a wide range of cell types. Heparitin Sulfate 99-114 fibroblast growth factor 2 Homo sapiens 28-33 10687947-8 2000 The possibility that FGF-2 activity can be manipulated through alterations in heparan sulfate-binding is currently being exploited in the development of clinical applications aimed at modulating either endogenous or administered FGF-2 activity. Heparitin Sulfate 78-93 fibroblast growth factor 2 Homo sapiens 21-26 10687947-8 2000 The possibility that FGF-2 activity can be manipulated through alterations in heparan sulfate-binding is currently being exploited in the development of clinical applications aimed at modulating either endogenous or administered FGF-2 activity. Heparitin Sulfate 78-93 fibroblast growth factor 2 Homo sapiens 229-234 10627288-2 2000 The heparan sulfate chains (HS) mediate stable high affinity binding of FGFs to their receptor tyrosine kinases (FR) and may specifically regulate FGF activity. Heparitin Sulfate 4-19 fibroblast growth factor 2 Homo sapiens 72-75 10673367-2 2000 When these cells are cultured in the presence of chlorate, an inhibitor of heparan sulfate (HS) sulfation, their proliferation is stimulated by both FGF-1 and FGF-2. Heparitin Sulfate 75-90 fibroblast growth factor 2 Homo sapiens 159-164 10673367-2 2000 When these cells are cultured in the presence of chlorate, an inhibitor of heparan sulfate (HS) sulfation, their proliferation is stimulated by both FGF-1 and FGF-2. Heparitin Sulfate 92-94 fibroblast growth factor 2 Homo sapiens 159-164 11097204-7 2000 In the third part the interaction of heparan sulfate chains with fibroblast growth factor-2 (FGF-2, basic FGF) as a prototype example for the interaction of heparin-binding cytokines with heparan sulfate proteoglycans is presented to illustrate the different levels of mutual dependence of the cytokine network and the ECM. Heparitin Sulfate 37-52 fibroblast growth factor 2 Homo sapiens 65-91 11097204-7 2000 In the third part the interaction of heparan sulfate chains with fibroblast growth factor-2 (FGF-2, basic FGF) as a prototype example for the interaction of heparin-binding cytokines with heparan sulfate proteoglycans is presented to illustrate the different levels of mutual dependence of the cytokine network and the ECM. Heparitin Sulfate 37-52 fibroblast growth factor 2 Homo sapiens 93-98 10627288-7 2000 Apparently, basement membrane and cell surface HSPGs differ in their ability to mediate the assembly of a FGF/FR signaling complex presumably due to structural differences of the heparan sulfate chains. Heparitin Sulfate 179-194 fibroblast growth factor 2 Homo sapiens 106-109 10521448-0 1999 Common binding sites for beta-amyloid fibrils and fibroblast growth factor-2 in heparan sulfate from human cerebral cortex. Heparitin Sulfate 80-95 fibroblast growth factor 2 Homo sapiens 50-76 10608853-10 1999 These results demonstrate that syndecan-3 expression is down-regulated during differentiation and the level of expression of membrane-bound heparan sulfate on myoblast surface is critical for fine modulation of responsiveness to FGF-2. Heparitin Sulfate 140-155 fibroblast growth factor 2 Homo sapiens 229-234 10521448-3 1999 On the other hand, heparan sulfate augments signaling of fibroblast growth factor-2 (FGF-2), a neuroprotective factor that antagonizes the neurotoxic effects of Abeta. Heparitin Sulfate 19-34 fibroblast growth factor 2 Homo sapiens 57-83 10521448-3 1999 On the other hand, heparan sulfate augments signaling of fibroblast growth factor-2 (FGF-2), a neuroprotective factor that antagonizes the neurotoxic effects of Abeta. Heparitin Sulfate 19-34 fibroblast growth factor 2 Homo sapiens 85-90 10521448-7 1999 The binding specificity of fibrillar Abeta is shared by FGF-2, and we here show that cerebral heparan sulfate domains selected for binding to Abeta-(1-40) fibrils bind also to FGF-2. Heparitin Sulfate 94-109 fibroblast growth factor 2 Homo sapiens 56-61 10521448-7 1999 The binding specificity of fibrillar Abeta is shared by FGF-2, and we here show that cerebral heparan sulfate domains selected for binding to Abeta-(1-40) fibrils bind also to FGF-2. Heparitin Sulfate 94-109 fibroblast growth factor 2 Homo sapiens 176-181 10446222-6 1999 Mutations of heparan sulfate attachment sites on S4 construct abolished syndecan-4-dependent augmentation of bFGF responses. Heparitin Sulfate 13-28 fibroblast growth factor 2 Homo sapiens 109-113 10530949-1 1999 It has been proposed that oligosaccharides corresponding to the so-called regular region of heparin/heparan sulfate (HS) bind to fibroblast growth factor-2 (FGF-2). Heparitin Sulfate 100-115 fibroblast growth factor 2 Homo sapiens 129-155 10530949-1 1999 It has been proposed that oligosaccharides corresponding to the so-called regular region of heparin/heparan sulfate (HS) bind to fibroblast growth factor-2 (FGF-2). Heparitin Sulfate 100-115 fibroblast growth factor 2 Homo sapiens 157-162 10389767-1 1999 Basic fibroblast growth factor (bFGF) is dependent on heparan sulphate for its ability to activate the cell surface signal transducing receptor. Heparitin Sulfate 54-70 fibroblast growth factor 2 Homo sapiens 0-30 10482449-1 1999 It has been suggested that the FGF-2 binding site on heparan sulfate chains is a trisulfated pentasaccharide containing three hexuronic acid units. Heparitin Sulfate 53-68 fibroblast growth factor 2 Homo sapiens 31-36 10389767-1 1999 Basic fibroblast growth factor (bFGF) is dependent on heparan sulphate for its ability to activate the cell surface signal transducing receptor. Heparitin Sulfate 54-70 fibroblast growth factor 2 Homo sapiens 32-36 10362842-1 1999 Fibroblast growth factors FGF-1 and FGF-2 mediate their biological effects via heparan sulfate-dependent interactions with cell surface FGF receptors. Heparitin Sulfate 79-94 fibroblast growth factor 2 Homo sapiens 36-41 10362842-2 1999 While the specific heparan sulfate domain binding to FGF-2 has been elucidated in some detail, limited information has been available concerning heparan sulfate structures involved in the recognition of FGF-1. Heparitin Sulfate 19-34 fibroblast growth factor 2 Homo sapiens 53-58 10336501-2 1999 Here we show that in contrast to heparin, cellular heparan sulfate forms a binary complex with FGFR that discriminates between FGF-1 and FGF-2. Heparitin Sulfate 51-66 fibroblast growth factor 2 Homo sapiens 137-142 10506830-11 1999 Moreover, it implies a novel transcriptional link that creates an FGF action-controlling autoregulatory loop between the heparan sulfate proteoglycans and the heparin-binding FGFs. Heparitin Sulfate 121-136 fibroblast growth factor 2 Homo sapiens 66-69 10224111-1 1999 The interaction of basic fibroblast growth factor (bFGF) with heparan sulfate (HS)/heparin has been shown to strongly enhance the activity of the growth factor although the mechanism of activation is unclear. Heparitin Sulfate 62-77 fibroblast growth factor 2 Homo sapiens 19-49 10224111-1 1999 The interaction of basic fibroblast growth factor (bFGF) with heparan sulfate (HS)/heparin has been shown to strongly enhance the activity of the growth factor although the mechanism of activation is unclear. Heparitin Sulfate 62-77 fibroblast growth factor 2 Homo sapiens 51-55 10224111-1 1999 The interaction of basic fibroblast growth factor (bFGF) with heparan sulfate (HS)/heparin has been shown to strongly enhance the activity of the growth factor although the mechanism of activation is unclear. Heparitin Sulfate 79-81 fibroblast growth factor 2 Homo sapiens 19-49 10224111-1 1999 The interaction of basic fibroblast growth factor (bFGF) with heparan sulfate (HS)/heparin has been shown to strongly enhance the activity of the growth factor although the mechanism of activation is unclear. Heparitin Sulfate 79-81 fibroblast growth factor 2 Homo sapiens 51-55 10051433-0 1999 Heparin and heparan sulphate protect basic fibroblast growth factor from non-enzymic glycosylation. Heparitin Sulfate 12-28 fibroblast growth factor 2 Homo sapiens 37-67 10051433-3 1999 In addition to intracellular localization, bFGF is also widely distributed in the extracellular matrix, primarily bound to heparan sulphate proteoglycans (HSPGs). Heparitin Sulfate 123-139 fibroblast growth factor 2 Homo sapiens 43-47 9704022-1 1998 Heparan sulphate from endothelial cells (ECHS) has been shown to bind to bFGF with a lower affinity than that seen for 3T3 fibroblast HS (FHS). Heparitin Sulfate 0-16 fibroblast growth factor 2 Homo sapiens 73-77 9748322-0 1998 Heparan sulfate proteoglycans control intracellular processing of bFGF in vascular smooth muscle cells. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 66-70 9722514-1 1998 The interaction of heparan sulfate (HS) with basic fibroblast growth factor (bFGF) is influential in enabling the growth factor to bind to its cell surface tyrosine kinase receptor. Heparitin Sulfate 19-34 fibroblast growth factor 2 Homo sapiens 45-75 9722514-1 1998 The interaction of heparan sulfate (HS) with basic fibroblast growth factor (bFGF) is influential in enabling the growth factor to bind to its cell surface tyrosine kinase receptor. Heparitin Sulfate 19-34 fibroblast growth factor 2 Homo sapiens 77-81 9722514-1 1998 The interaction of heparan sulfate (HS) with basic fibroblast growth factor (bFGF) is influential in enabling the growth factor to bind to its cell surface tyrosine kinase receptor. Heparitin Sulfate 36-38 fibroblast growth factor 2 Homo sapiens 45-75 9722514-1 1998 The interaction of heparan sulfate (HS) with basic fibroblast growth factor (bFGF) is influential in enabling the growth factor to bind to its cell surface tyrosine kinase receptor. Heparitin Sulfate 36-38 fibroblast growth factor 2 Homo sapiens 77-81 9199199-2 1997 The binding site in heparin and heparan sulfate for fibroblast growth factor-2 (basic fibroblast growth factor) has been described as rich in glucosamine-2-sulfate 1-->4 linked to iduronic acid-2-sulfate. Heparitin Sulfate 32-47 fibroblast growth factor 2 Homo sapiens 52-78 9516424-0 1998 Interaction of heparan sulfate from mammary cells with acidic fibroblast growth factor (FGF) and basic FGF. Heparitin Sulfate 15-30 fibroblast growth factor 2 Homo sapiens 97-106 9516424-1 1998 Regulation of the activity of basic FGF by high and low affinity binding sites in heparan sulfate. Heparitin Sulfate 82-97 fibroblast growth factor 2 Homo sapiens 30-39 9516424-2 1998 We have determined the relationship between the binding sites for acidic fibroblast growth factor (aFGF) and basic FGF (bFGF) in heparan sulfate (HS) prepared from a panel of mammary cell lines and the ability of the HS to activate aFGF and bFGF in DNA synthesis assays. Heparitin Sulfate 129-144 fibroblast growth factor 2 Homo sapiens 109-118 9516424-2 1998 We have determined the relationship between the binding sites for acidic fibroblast growth factor (aFGF) and basic FGF (bFGF) in heparan sulfate (HS) prepared from a panel of mammary cell lines and the ability of the HS to activate aFGF and bFGF in DNA synthesis assays. Heparitin Sulfate 129-144 fibroblast growth factor 2 Homo sapiens 120-124 9516424-2 1998 We have determined the relationship between the binding sites for acidic fibroblast growth factor (aFGF) and basic FGF (bFGF) in heparan sulfate (HS) prepared from a panel of mammary cell lines and the ability of the HS to activate aFGF and bFGF in DNA synthesis assays. Heparitin Sulfate 146-148 fibroblast growth factor 2 Homo sapiens 109-118 9516424-2 1998 We have determined the relationship between the binding sites for acidic fibroblast growth factor (aFGF) and basic FGF (bFGF) in heparan sulfate (HS) prepared from a panel of mammary cell lines and the ability of the HS to activate aFGF and bFGF in DNA synthesis assays. Heparitin Sulfate 146-148 fibroblast growth factor 2 Homo sapiens 120-124 9623978-4 1998 The ectodomain potently inhibits heparin-mediated FGF-2 mitogenicity because of the poorly sulfated domains in its heparin sulfate chains. Heparitin Sulfate 115-130 fibroblast growth factor 2 Homo sapiens 50-55 9623978-5 1998 Degradation of these regions by platelet heparanase produces heparin-like heparin sulfate fragments that markedly activate FGF-2 mitogenicity and are found in wound fluids. Heparitin Sulfate 74-89 fibroblast growth factor 2 Homo sapiens 123-128 9473690-3 1998 In a previous study, we showed that basic fibroblast growth factor (bFGF), another heparin-binding protein is increased in Alzheimer"s disease (AD), and appears to be associated with the heparan-sulfate proteoglycans bound to B/A4 amyloid (Biochem. Heparitin Sulfate 187-202 fibroblast growth factor 2 Homo sapiens 36-66 9473690-3 1998 In a previous study, we showed that basic fibroblast growth factor (bFGF), another heparin-binding protein is increased in Alzheimer"s disease (AD), and appears to be associated with the heparan-sulfate proteoglycans bound to B/A4 amyloid (Biochem. Heparitin Sulfate 187-202 fibroblast growth factor 2 Homo sapiens 68-72 9407232-9 1997 CONCLUSIONS: These experiments demonstrate that FGF-2 is effective in promoting herpetic epithelial ulcer healing, either due to its proliferative effects on epithelial cells or indirectly by occupying the sites on cell surface heparan sulfate necessary for the attachment of the virion. Heparitin Sulfate 228-243 fibroblast growth factor 2 Homo sapiens 48-53 9258342-2 1997 Binding of bFGF to heparin or heparan sulfate has been demonstrated to both stimulate and inhibit growth factor activity. Heparitin Sulfate 30-45 fibroblast growth factor 2 Homo sapiens 11-15 9278150-2 1997 In the present study the influence of undersulfated heparan sulfate on the expression of basic fibroblast growth factor and coronary smooth muscle cell (cSMC) proliferation was investigated. Heparitin Sulfate 52-67 fibroblast growth factor 2 Homo sapiens 89-119 9278150-12 1997 Our results demonstrate an inverse association between the sulfation of heparan sulfate and the expression of bFGF. Heparitin Sulfate 72-87 fibroblast growth factor 2 Homo sapiens 110-114 9107173-2 1997 Among the growth factors that promote SMC proliferation is basic fibroblast growth factor (bFGF), which is characterized by a high affinity for heparin and is associated with heparan sulfate on cell surfaces and extracellular matrices. Heparitin Sulfate 175-190 fibroblast growth factor 2 Homo sapiens 59-89 9094999-0 1997 Differential binding of fibroblast growth factor-2 and -7 to basement membrane heparan sulfate: comparison of normal and abnormal human tissues. Heparitin Sulfate 79-94 fibroblast growth factor 2 Homo sapiens 24-57 9094999-4 1997 Using biotinylated FGF-2 and FGF-7 (KGF) as probes, we have identified specific interactions between FGFs and heparan sulfates in human tissues. Heparitin Sulfate 110-126 fibroblast growth factor 2 Homo sapiens 19-24 9094999-7 1997 Although FGF-2 strongly binds to basement membrane heparan sulfate in skin and most other tissue sites examined, FGF-7 fails to bind to basement membrane heparan sulfate in most locations. Heparitin Sulfate 51-66 fibroblast growth factor 2 Homo sapiens 9-14 9107173-2 1997 Among the growth factors that promote SMC proliferation is basic fibroblast growth factor (bFGF), which is characterized by a high affinity for heparin and is associated with heparan sulfate on cell surfaces and extracellular matrices. Heparitin Sulfate 175-190 fibroblast growth factor 2 Homo sapiens 91-95 9093910-11 1997 We demonstrated that HBL-100 and MDA-MB-231 cells bind more FGF-2 to their heparan sulfate proteoglycans than MCF-7 cells. Heparitin Sulfate 75-90 fibroblast growth factor 2 Homo sapiens 60-65 8842489-7 1996 In the present review we focus on the involvement of HS in basic FGF (bFGF) receptor binding and mitogenic activity and its modulation by species of heparin, HS, and synthetic polyanionic "heparin-mimicking" compounds. Heparitin Sulfate 53-55 fibroblast growth factor 2 Homo sapiens 59-68 9000592-1 1997 A number of growth factors, including members of the fibroblast growth factor (FGF) family - hepatocyte growth factor, vascular endothelial growth factor and heparin-binding epidermal growth factor - are dependent on heparan sulphate (HS) for biological activity mediated through their high-affinity signal-transducing receptors. Heparitin Sulfate 217-233 fibroblast growth factor 2 Homo sapiens 79-82 9000592-1 1997 A number of growth factors, including members of the fibroblast growth factor (FGF) family - hepatocyte growth factor, vascular endothelial growth factor and heparin-binding epidermal growth factor - are dependent on heparan sulphate (HS) for biological activity mediated through their high-affinity signal-transducing receptors. Heparitin Sulfate 235-237 fibroblast growth factor 2 Homo sapiens 79-82 9000592-6 1997 Here, we show that the mitogenic response to bFGF is dependent on the presence of heparan sulphate. Heparitin Sulfate 82-98 fibroblast growth factor 2 Homo sapiens 45-49 8816920-1 1996 The interaction of basic FGF (bFGF) with heparin, heparan sulfate and related sugars can potentiate or antagonize bFGF activity, depending on the size of the saccharide used. Heparitin Sulfate 50-65 fibroblast growth factor 2 Homo sapiens 19-28 8816920-1 1996 The interaction of basic FGF (bFGF) with heparin, heparan sulfate and related sugars can potentiate or antagonize bFGF activity, depending on the size of the saccharide used. Heparitin Sulfate 50-65 fibroblast growth factor 2 Homo sapiens 30-34 8816920-1 1996 The interaction of basic FGF (bFGF) with heparin, heparan sulfate and related sugars can potentiate or antagonize bFGF activity, depending on the size of the saccharide used. Heparitin Sulfate 50-65 fibroblast growth factor 2 Homo sapiens 114-118 8816920-6 1996 We report that the ability of sulfated malto-oligosaccharides to block binding of bFGF to heparan sulfate was dependent on the size (at least a tetrasaccharide is required), and the degree of sulfation. Heparitin Sulfate 90-105 fibroblast growth factor 2 Homo sapiens 82-86 8842489-7 1996 In the present review we focus on the involvement of HS in basic FGF (bFGF) receptor binding and mitogenic activity and its modulation by species of heparin, HS, and synthetic polyanionic "heparin-mimicking" compounds. Heparitin Sulfate 53-55 fibroblast growth factor 2 Homo sapiens 70-74 8617759-1 1996 The growth promoting activity of the subendothelial extracellular matrix (ECM) is attributed to sequestration of basic fibroblast growth factor (bFGF) by heparan sulfate proteoglycans and its regulated release by heparin-like molecules and heparan sulfate (HS) degrading enzymes. Heparitin Sulfate 240-255 fibroblast growth factor 2 Homo sapiens 145-149 8626565-7 1996 We showed also that perlecan bound bFGF specifically by the heparan sulfate chains located on the amino-terminal domain I. Heparitin Sulfate 60-75 fibroblast growth factor 2 Homo sapiens 35-39 8626565-10 1996 Our findings provide direct evidence that bFGF binds to heparan sulfate sequences attached to domain I and support the hypothesis that perlecan represents a major storage site for this growth factor in the blood vessel wall. Heparitin Sulfate 56-71 fibroblast growth factor 2 Homo sapiens 42-46 8609172-1 1996 The formation of distinctive basic FGF-heparan sulfate complexes is essential for the binding of bFGF to its cognate receptor. Heparitin Sulfate 39-54 fibroblast growth factor 2 Homo sapiens 35-38 8609172-1 1996 The formation of distinctive basic FGF-heparan sulfate complexes is essential for the binding of bFGF to its cognate receptor. Heparitin Sulfate 39-54 fibroblast growth factor 2 Homo sapiens 97-101 8617759-1 1996 The growth promoting activity of the subendothelial extracellular matrix (ECM) is attributed to sequestration of basic fibroblast growth factor (bFGF) by heparan sulfate proteoglycans and its regulated release by heparin-like molecules and heparan sulfate (HS) degrading enzymes. Heparitin Sulfate 154-169 fibroblast growth factor 2 Homo sapiens 113-143 8617759-1 1996 The growth promoting activity of the subendothelial extracellular matrix (ECM) is attributed to sequestration of basic fibroblast growth factor (bFGF) by heparan sulfate proteoglycans and its regulated release by heparin-like molecules and heparan sulfate (HS) degrading enzymes. Heparitin Sulfate 154-169 fibroblast growth factor 2 Homo sapiens 145-149 8617759-1 1996 The growth promoting activity of the subendothelial extracellular matrix (ECM) is attributed to sequestration of basic fibroblast growth factor (bFGF) by heparan sulfate proteoglycans and its regulated release by heparin-like molecules and heparan sulfate (HS) degrading enzymes. Heparitin Sulfate 257-259 fibroblast growth factor 2 Homo sapiens 113-143 8617759-1 1996 The growth promoting activity of the subendothelial extracellular matrix (ECM) is attributed to sequestration of basic fibroblast growth factor (bFGF) by heparan sulfate proteoglycans and its regulated release by heparin-like molecules and heparan sulfate (HS) degrading enzymes. Heparitin Sulfate 257-259 fibroblast growth factor 2 Homo sapiens 145-149 8536692-7 1995 In control cells, cell-associated heparan sulfate accounts for about 30% of the total glycosaminoglycans, whereas under the influence of bFGF the amount of heparan sulfate increases to approximately 60%. Heparitin Sulfate 156-171 fibroblast growth factor 2 Homo sapiens 137-141 8547648-0 1996 Human leukemia cell lines bind basic fibroblast growth factor (FGF) on FGF receptors and heparan sulfates: downmodulation of FGF receptors by phorbol ester. Heparitin Sulfate 89-105 fibroblast growth factor 2 Homo sapiens 31-61 8536692-11 1995 The results suggest a bFGF-dependent change in the rate of synthesis and structural features of the membrane-associated heparan sulfate in corneal endothelial cells. Heparitin Sulfate 120-135 fibroblast growth factor 2 Homo sapiens 22-26 8536692-12 1995 The modification of the heparan sulfate structure could influence its bFGF-binding and antiproliferative activity. Heparitin Sulfate 24-39 fibroblast growth factor 2 Homo sapiens 70-74 8536692-8 1995 At the same time, the molecular structure of the heparan sulfate molecule undergoes bFGF-specific changes as indicated by the [35S]oligosaccharide pattern generated by heparitinase I degradation. Heparitin Sulfate 49-64 fibroblast growth factor 2 Homo sapiens 84-88 8594987-1 1995 Basic fibroblast growth factor (bFGF) is a member of the heparin-binding growth factor family that interacts with cell surface heparan sulfate (HS) proteoglycans and extracellular matrix heparin. Heparitin Sulfate 127-142 fibroblast growth factor 2 Homo sapiens 0-30 8594987-1 1995 Basic fibroblast growth factor (bFGF) is a member of the heparin-binding growth factor family that interacts with cell surface heparan sulfate (HS) proteoglycans and extracellular matrix heparin. Heparitin Sulfate 144-146 fibroblast growth factor 2 Homo sapiens 32-36 8594987-2 1995 Here we report the development of a simple and sensitive assay that used biotinylated HS or heparin to bind to bFGF coated onto 96-well microtiter plates. Heparitin Sulfate 86-88 fibroblast growth factor 2 Homo sapiens 111-115 8594987-1 1995 Basic fibroblast growth factor (bFGF) is a member of the heparin-binding growth factor family that interacts with cell surface heparan sulfate (HS) proteoglycans and extracellular matrix heparin. Heparitin Sulfate 127-142 fibroblast growth factor 2 Homo sapiens 32-36 8594987-1 1995 Basic fibroblast growth factor (bFGF) is a member of the heparin-binding growth factor family that interacts with cell surface heparan sulfate (HS) proteoglycans and extracellular matrix heparin. Heparitin Sulfate 144-146 fibroblast growth factor 2 Homo sapiens 0-30 8746075-6 1995 The failure of the stem cells to differentiate completely to myoepithelial cells in carcinomas greatly reduces the heparan sulphate proteoglycan sink used to sequester to bFGF in normal glands and also removes the possibility of eliminating cells by terminal differentiation, both processes possibly contributing to the uncontrolled growth of the malignant breast cell. Heparitin Sulfate 115-131 fibroblast growth factor 2 Homo sapiens 171-175 8590594-1 1995 High-affinity binding of basic fibroblast growth factor (bFGF) to the tyrosine kinase receptor requires cell-surface heparan sulfate proteoglycan or exogenous addition of heparin. Heparitin Sulfate 117-132 fibroblast growth factor 2 Homo sapiens 25-55 8590594-1 1995 High-affinity binding of basic fibroblast growth factor (bFGF) to the tyrosine kinase receptor requires cell-surface heparan sulfate proteoglycan or exogenous addition of heparin. Heparitin Sulfate 117-132 fibroblast growth factor 2 Homo sapiens 57-61 8548124-0 1995 Basic fibroblast growth factor-heparan sulphate complex in the human dialysis-related amyloidosis. Heparitin Sulfate 31-47 fibroblast growth factor 2 Homo sapiens 0-30 7699017-0 1994 Heparan sulfate proteoglycans as transducers of FGF-2 signalling. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 48-53 7827407-1 1994 The biological activity of basic fibroblast growth factor (bFGF) is influenced greatly by direct binding to heparin and heparan sulphate (HS). Heparitin Sulfate 120-136 fibroblast growth factor 2 Homo sapiens 27-57 7967508-0 1994 Basic fibroblast growth factor-binding domain of heparan sulfate in the human glomerulosclerosis and renal tubulointerstitial fibrosis. Heparitin Sulfate 49-64 fibroblast growth factor 2 Homo sapiens 0-30 8050494-2 1994 Heparinase II treatment of HeLa and CCL 39 cells resulted in a decrease of bFGF binding by 96 and 57%, respectively, indicating that heparan sulfate molecules were involved in bFGF binding. Heparitin Sulfate 133-148 fibroblast growth factor 2 Homo sapiens 75-79 8050494-2 1994 Heparinase II treatment of HeLa and CCL 39 cells resulted in a decrease of bFGF binding by 96 and 57%, respectively, indicating that heparan sulfate molecules were involved in bFGF binding. Heparitin Sulfate 133-148 fibroblast growth factor 2 Homo sapiens 176-180 7827407-1 1994 The biological activity of basic fibroblast growth factor (bFGF) is influenced greatly by direct binding to heparin and heparan sulphate (HS). Heparitin Sulfate 120-136 fibroblast growth factor 2 Homo sapiens 59-63 7827407-1 1994 The biological activity of basic fibroblast growth factor (bFGF) is influenced greatly by direct binding to heparin and heparan sulphate (HS). Heparitin Sulfate 138-140 fibroblast growth factor 2 Homo sapiens 27-57 7827407-1 1994 The biological activity of basic fibroblast growth factor (bFGF) is influenced greatly by direct binding to heparin and heparan sulphate (HS). Heparitin Sulfate 138-140 fibroblast growth factor 2 Homo sapiens 59-63 7827407-3 1994 We had previously demonstrated that fragments > 6 mer can inhibit the interaction between cell surface heparan sulphate proteoglycan (HSPG) and bFGF, and bFGF-induced proliferation of adrenocortical endothelial (ACE) cells. Heparitin Sulfate 106-122 fibroblast growth factor 2 Homo sapiens 147-151 7827407-3 1994 We had previously demonstrated that fragments > 6 mer can inhibit the interaction between cell surface heparan sulphate proteoglycan (HSPG) and bFGF, and bFGF-induced proliferation of adrenocortical endothelial (ACE) cells. Heparitin Sulfate 106-122 fibroblast growth factor 2 Homo sapiens 157-161 8161780-3 1994 The bFGF-proteoglycan complex is biologically active and is released by addition of exogenous phosphatidylinositol-specific phospholipase C. In this study, we show the presence of an endogenous GPI-specific phospholipase D (GPI-PLD) that releases the bFGF-binding heparan sulfate proteoglycan and the variant surface glycoprotein (a model GPI-anchored protein) from BM cultures. Heparitin Sulfate 264-279 fibroblast growth factor 2 Homo sapiens 4-8 8448389-0 1993 Selective impairment of the synthesis of basic fibroblast growth factor binding domains of heparan sulphate in a COS cell mutant defective in N-sulphotransferase. Heparitin Sulfate 91-107 fibroblast growth factor 2 Homo sapiens 41-71 8142385-2 1994 Previous reports have implicated the binding of heparin, or heparan sulfate, to FGF as essential for FGF-mediated signal transduction and mitogenicity. Heparitin Sulfate 60-75 fibroblast growth factor 2 Homo sapiens 80-83 8142385-2 1994 Previous reports have implicated the binding of heparin, or heparan sulfate, to FGF as essential for FGF-mediated signal transduction and mitogenicity. Heparitin Sulfate 60-75 fibroblast growth factor 2 Homo sapiens 101-104 8226930-1 1993 Experiments based on interaction in free solution between basic fibroblast growth factor (FGF-2) and saccharides related to heparin/heparan sulfate showed that the growth factor binds to heparin and to selectively glucosaminyl 6-O-desulfated heparin but poorly to iduronosyl 2-O-desulfated heparin. Heparitin Sulfate 132-147 fibroblast growth factor 2 Homo sapiens 90-95 8497125-9 1993 Areas of deposition of bFGF in the ECM partly corresponded to areas of increased immunoreactivity for heparan sulphate proteoglycan. Heparitin Sulfate 102-118 fibroblast growth factor 2 Homo sapiens 23-27 8288646-4 1994 This paper describes further work on the binding properties of HS saccharides and their capacity to mediate bFGF activity in a mitogenesis assay in which responsiveness is dependent on the addition of HS or heparin. Heparitin Sulfate 63-65 fibroblast growth factor 2 Homo sapiens 108-112 8507869-0 1993 Thrombin-induced release of active basic fibroblast growth factor-heparan sulfate complexes from subendothelial extracellular matrix. Heparitin Sulfate 66-81 fibroblast growth factor 2 Homo sapiens 35-65 8508806-7 1993 The capacity of different GAGs to protect bFGF from proteolytic cleavage decreases in the following order: heparin > heparan sulfate > dermatan sulfate = chondroitin sulfates A and C > hyaluronic acid = K5 polysaccharide, indicating that both the degree of sulfation and the backbone structure of GAG modulate its interaction with bFGF. Heparitin Sulfate 120-135 fibroblast growth factor 2 Homo sapiens 42-46 1331122-9 1992 The subpopulation of heparan sulfate proteoglycans capable to bind bFGF was also largely increased after ACTH or TGF beta treatment and paralleled the variation in overall proteoheparan sulfate synthesis. Heparitin Sulfate 21-36 fibroblast growth factor 2 Homo sapiens 67-71 1472758-0 1992 Heparan sulphate in the binding and activation of basic fibroblast growth factor. Heparitin Sulfate 0-16 fibroblast growth factor 2 Homo sapiens 50-80 1279952-4 1992 (3) FGFs and thrombospondin are stored in the extracellular matrix bound to heparan sulfate; fragments of heparin or heparan sulfate may act as natural chaperones to shuttle bFGF or other growth factors to different cellular compartments. Heparitin Sulfate 117-132 fibroblast growth factor 2 Homo sapiens 174-178 1378313-2 1992 Heparin and heparan sulfate protect bFGF from inactivation by heat, extreme pH and protease degradation. Heparitin Sulfate 12-27 fibroblast growth factor 2 Homo sapiens 36-40 1378313-3 1992 At the cellular level, bFGF binds to heparan sulfate on the cell surface. Heparitin Sulfate 37-52 fibroblast growth factor 2 Homo sapiens 23-27 1536567-3 1992 Heparan sulfate, a component of the endothelial extracellular matrix, was also observed to bind to bFGF and induce a similar conformational change to that observed for heparin. Heparitin Sulfate 0-15 fibroblast growth factor 2 Homo sapiens 99-103 1379245-0 1992 Repression of myogenic differentiation by aFGF, bFGF, and K-FGF is dependent on cellular heparan sulfate. Heparitin Sulfate 89-104 fibroblast growth factor 2 Homo sapiens 48-52 1587820-0 1992 Identification of the basic fibroblast growth factor binding sequence in fibroblast heparan sulfate. Heparitin Sulfate 84-99 fibroblast growth factor 2 Homo sapiens 22-52 1587820-1 1992 The structural properties of fibroblast heparan sulfate (HS) that are necessary for it to bind strongly to basic fibroblast growth factor (bFGF) have been investigated using bFGF affinity chromatography. Heparitin Sulfate 40-55 fibroblast growth factor 2 Homo sapiens 107-137 1587820-1 1992 The structural properties of fibroblast heparan sulfate (HS) that are necessary for it to bind strongly to basic fibroblast growth factor (bFGF) have been investigated using bFGF affinity chromatography. Heparitin Sulfate 40-55 fibroblast growth factor 2 Homo sapiens 139-143 1587820-1 1992 The structural properties of fibroblast heparan sulfate (HS) that are necessary for it to bind strongly to basic fibroblast growth factor (bFGF) have been investigated using bFGF affinity chromatography. Heparitin Sulfate 40-55 fibroblast growth factor 2 Homo sapiens 174-178 1587820-1 1992 The structural properties of fibroblast heparan sulfate (HS) that are necessary for it to bind strongly to basic fibroblast growth factor (bFGF) have been investigated using bFGF affinity chromatography. Heparitin Sulfate 57-59 fibroblast growth factor 2 Homo sapiens 107-137 1587820-1 1992 The structural properties of fibroblast heparan sulfate (HS) that are necessary for it to bind strongly to basic fibroblast growth factor (bFGF) have been investigated using bFGF affinity chromatography. Heparitin Sulfate 57-59 fibroblast growth factor 2 Homo sapiens 139-143 1587820-1 1992 The structural properties of fibroblast heparan sulfate (HS) that are necessary for it to bind strongly to basic fibroblast growth factor (bFGF) have been investigated using bFGF affinity chromatography. Heparitin Sulfate 57-59 fibroblast growth factor 2 Homo sapiens 174-178 1711529-4 1991 We have found that bFGF is bound to heparan sulfate (HS) in the ECM and is released in an active form when the ECM-HS is degraded by heparanase expressed by normal and malignant cells (i.e. platelets, neutrophils, lymphoma cells). Heparitin Sulfate 36-51 fibroblast growth factor 2 Homo sapiens 19-23 1926336-1 1991 Basic fibroblast growth factor, (bFGF), promotes the formation of new blood capillaries and is sequestered and protected by binding to heparan sulfate (HS), both on the cell surface and in the extracellular matrix. Heparitin Sulfate 135-150 fibroblast growth factor 2 Homo sapiens 33-37 1926336-1 1991 Basic fibroblast growth factor, (bFGF), promotes the formation of new blood capillaries and is sequestered and protected by binding to heparan sulfate (HS), both on the cell surface and in the extracellular matrix. Heparitin Sulfate 152-154 fibroblast growth factor 2 Homo sapiens 33-37 1646484-2 1991 Prevention of binding between cell surface heparan sulfate and bFGF (i) substantially reduces binding of fibroblast growth factor to its cell-surface receptors, (ii) blocks the ability of bFGF to support the growth of Swiss 3T3 fibroblasts, and (iii) induces terminal differentiation of MM14 skeletal muscle cells, which is normally repressed by fibroblast growth factor. Heparitin Sulfate 43-58 fibroblast growth factor 2 Homo sapiens 188-192 1646484-3 1991 These results indicate that cell surface heparan sulfate is directly involved in bFGF cell signaling. Heparitin Sulfate 41-56 fibroblast growth factor 2 Homo sapiens 81-85 1646484-0 1991 Requirement of heparan sulfate for bFGF-mediated fibroblast growth and myoblast differentiation. Heparitin Sulfate 15-30 fibroblast growth factor 2 Homo sapiens 35-39 1711529-4 1991 We have found that bFGF is bound to heparan sulfate (HS) in the ECM and is released in an active form when the ECM-HS is degraded by heparanase expressed by normal and malignant cells (i.e. platelets, neutrophils, lymphoma cells). Heparitin Sulfate 53-55 fibroblast growth factor 2 Homo sapiens 19-23 1711916-4 1990 Increased production of plasmin may be responsible for the release of soluble complexes of heparan sulfate-bFGF which may be the active form of bFGF. Heparitin Sulfate 91-106 fibroblast growth factor 2 Homo sapiens 107-111 1711656-7 1991 These results indicate that AD lesions contain bFGF-binding sites and that the chemical substrate for bFGF binding to AD lesions was heparan sulfate. Heparitin Sulfate 133-148 fibroblast growth factor 2 Homo sapiens 102-106 1711916-4 1990 Increased production of plasmin may be responsible for the release of soluble complexes of heparan sulfate-bFGF which may be the active form of bFGF. Heparitin Sulfate 91-106 fibroblast growth factor 2 Homo sapiens 144-148 35236856-0 2022 Phase separation on cell surface facilitates bFGF signal transduction with heparan sulphate. Heparitin Sulfate 75-91 fibroblast growth factor 2 Homo sapiens 45-49 1705486-7 1990 Our results indicate that bFGF is bound to heparan sulfate (HS) in the ECM and is released in an active form when the ECM-HS is degraded by cellular heparanase. Heparitin Sulfate 43-58 fibroblast growth factor 2 Homo sapiens 26-30 1705486-7 1990 Our results indicate that bFGF is bound to heparan sulfate (HS) in the ECM and is released in an active form when the ECM-HS is degraded by cellular heparanase. Heparitin Sulfate 60-62 fibroblast growth factor 2 Homo sapiens 26-30 35433697-9 2022 Membrane-inserted FGF2 oligomers are recognized as translocation intermediates that are resolved at the outer plasma membrane leaflet by glypican-1, a heparan sulfate proteoglycan that captures and disassembles FGF2 oligomers on cell surfaces. Heparitin Sulfate 151-166 fibroblast growth factor 2 Homo sapiens 18-22 35433697-9 2022 Membrane-inserted FGF2 oligomers are recognized as translocation intermediates that are resolved at the outer plasma membrane leaflet by glypican-1, a heparan sulfate proteoglycan that captures and disassembles FGF2 oligomers on cell surfaces. Heparitin Sulfate 151-166 fibroblast growth factor 2 Homo sapiens 211-215 35348113-5 2022 We provide first insights into the structural basis for GPC1-dependent FGF2 secretion, identifying disaccharides with N-linked sulfate groups to be enriched in the heparan sulfate chains of GPC1 to which FGF2 binds with high affinity. Heparitin Sulfate 164-179 fibroblast growth factor 2 Homo sapiens 71-75 35348113-5 2022 We provide first insights into the structural basis for GPC1-dependent FGF2 secretion, identifying disaccharides with N-linked sulfate groups to be enriched in the heparan sulfate chains of GPC1 to which FGF2 binds with high affinity. Heparitin Sulfate 164-179 fibroblast growth factor 2 Homo sapiens 204-208