PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2842255-4 1988 In contrast, neutrophils preincubated with rH GM-CSF exhibited reduced chemotaxis under agarose in response to a gradient of f Met-Leu-Phe. Sepharose 88-95 colony stimulating factor 2 Homo sapiens 46-52 1904673-2 1991 Nineteen patients presented free kappa or lambda light chain bands in CSF after agarose isoelectric focusing, protein transfer to nitrocellulose and immunostaining with specific antisera. Sepharose 80-87 colony stimulating factor 2 Homo sapiens 70-73 22303054-9 2011 According to agarose gel electrophoresis, full complexation was obtained at 0.1/1 and higher chitosan/pGM-CSF ratios. Sepharose 13-20 colony stimulating factor 2 Homo sapiens 106-109 2147182-5 1990 Approximately 2 g of the placental membrane extract was subjected to a biotinylated GM-CSF-fixed streptavidin-agarose column, resulting in a single major band at 70 kDa on a silver-stained sodium dodecyl sulfate gel. Sepharose 110-117 colony stimulating factor 2 Homo sapiens 84-90 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 195-204 colony stimulating factor 2 Homo sapiens 24-30 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 277-286 colony stimulating factor 2 Homo sapiens 24-30 2157779-5 1990 The GM-CSF receptor is a glycoprotein which binds to wheat germ agglutinin-sepharose. Sepharose 75-84 colony stimulating factor 2 Homo sapiens 4-10 2783250-3 1989 The IgG fractions from normal rabbit serum and the anti-CSF serum were bound to cyanogen bromide-activated sepharose. Sepharose 107-116 colony stimulating factor 2 Homo sapiens 56-59 2783250-8 1989 In a further purification step, the CSF was retained selectively by concanavalin A sepharose and eluted with alpha-methylglucoside. Sepharose 83-92 colony stimulating factor 2 Homo sapiens 36-39 3135686-1 1988 Colony-stimulating factor (CSF) was partially purified from urine of patients with aplastic anemia using DEAE-cellulose and concanavalin A-Sepharose. Sepharose 139-148 colony stimulating factor 2 Homo sapiens 0-25 3135686-1 1988 Colony-stimulating factor (CSF) was partially purified from urine of patients with aplastic anemia using DEAE-cellulose and concanavalin A-Sepharose. Sepharose 139-148 colony stimulating factor 2 Homo sapiens 27-30 3654180-4 1987 Polyacrylamide isoelectric focusing appeared more sensitive than agarose electrophoresis for the detection of abnormal CSF fractions. Sepharose 65-72 colony stimulating factor 2 Homo sapiens 119-122 2478092-0 1988 [Electrophoresis of unconcentrated CSF proteins in agarose gels. Sepharose 51-58 colony stimulating factor 2 Homo sapiens 35-38 3115913-1 1987 We demonstrate the possibility of a complete routine study on unconcentrated CSF from patients with Multiple Sclerosis (MS) using agarose isoelectric focusing (AIEF) followed by avidin-biotin-peroxidase complex staining to detect IgG oligoclonal bands (OB) and free light chains (FLC). Sepharose 130-137 colony stimulating factor 2 Homo sapiens 77-80 6979358-2 1982 A quantity of 100 X 10(6) units of purified anti-CSF was coupled to cyanogen bromide activated Sepharose 4B; colony assays revealed complete binding of the antibodies to the gel. Sepharose 95-107 colony stimulating factor 2 Homo sapiens 49-52 2990610-2 1985 The human active granulocyte-macrophage colony-stimulating factor (GM-CSF) for day 7 CFU-GM and the GM-DF for WEHI-3B(D+) and for HL-60 are not separable by acrylamide agarose column chromatography, eluting at an apparent molecular weight between 20,000 and 35,000 daltons, or by isoelectric focusing (isoelectric point, pH 5.4). Sepharose 168-175 colony stimulating factor 2 Homo sapiens 67-73 6327252-2 1984 CSF proteins in 107 children ranging from 3 to 24 months of age were analyzed by means of quantitative zone electrophoresis on agarose gel. Sepharose 127-134 colony stimulating factor 2 Homo sapiens 0-3 230687-4 1979 Oligoclonal IgG was detected by agarose electrophoresis of the CSF in four of the five patients with a local synthesis of virus antibodies. Sepharose 32-39 colony stimulating factor 2 Homo sapiens 63-66 6971130-1 1980 Urinary colony stimulating factor (CSF) from human leukemic urine is retained on propylamine-agarose hydrophobic chromatography; about 70% of total activity is eluted by the addition of 1 M NaCl with significant increase in specific activity. Sepharose 93-100 colony stimulating factor 2 Homo sapiens 35-38 7042918-0 1982 Agarose isoelectric focusing of unconcentrated CSF and radioimmunofixation for detection of oligoclonal bands in patients with multiple sclerosis and other neurological diseases. Sepharose 0-7 colony stimulating factor 2 Homo sapiens 47-50 7265171-5 1981 CSF from 30 of 40 MS patients (75%) showed 1-4 oligoclonal IgG bands in agarose gel electrophoresis (PANAGEL), however, the resolution of bands was generally poor. Sepharose 72-79 colony stimulating factor 2 Homo sapiens 0-3 7011523-1 1980 Agarose and/or cellulose acetate electrophoresis was performed on the CSF of one thousand patients. Sepharose 0-7 colony stimulating factor 2 Homo sapiens 70-73 115379-0 1979 Immunoglobulin class and light chain type of oligoclonal bands in CSF in multiple sclerosis determined by agarose gel electrophoresis and immunofixation. Sepharose 106-113 colony stimulating factor 2 Homo sapiens 66-69 115379-1 1979 Agarose gel electrophoresis and immunofixation of CSF and serum from 39 patients with multiple sclerosis (MS) revealed oligoclonal IgG in the CSF in all cases and oligoclonal IgA and IgM in 1 patient each. Sepharose 0-7 colony stimulating factor 2 Homo sapiens 142-145 50707-3 1975 Multiple discrete IgG bands were demonstrated by agarose electrophoresis in serum and in CSF during the first 2 weeks after onset of the neurological symptoms, and also after week 2 and still during week 12 after onset. Sepharose 49-56 colony stimulating factor 2 Homo sapiens 89-92 337461-7 1977 Oligoclonal IgG was found in CSF by agarose gel electrophoresis in 88% of the patients. Sepharose 36-43 colony stimulating factor 2 Homo sapiens 29-32 1082142-1 1975 Biological activities associated with colony-stimulating factor (CSF) from human leukemic urine were found to be selectively retained on an affinity adsorbent of Con A-Sepharose. Sepharose 168-177 colony stimulating factor 2 Homo sapiens 38-63 1082142-1 1975 Biological activities associated with colony-stimulating factor (CSF) from human leukemic urine were found to be selectively retained on an affinity adsorbent of Con A-Sepharose. Sepharose 168-177 colony stimulating factor 2 Homo sapiens 65-68 1082142-5 1975 Affinity chromatography of CSF on a Blue Dextran-Sepharose adsorbent was found to be an effective method for removing albumin, a major protein contaminant in urinary preparations. Sepharose 49-58 colony stimulating factor 2 Homo sapiens 27-30 31050-2 1978 In selected cases with unsure CSF oligoclonal bands on agarose gel electrophoresis, isoelectric focusing displayed definite oligoclonal bands. Sepharose 55-62 colony stimulating factor 2 Homo sapiens 30-33 807868-9 1975 For a laboratory with special interest in multiple sclerosis, agarose gel-electrophoresis of concentrated CSF appears to be the test of choice. Sepharose 62-69 colony stimulating factor 2 Homo sapiens 106-109 30593382-4 2019 In this study, the porcine GM-CSF (pGM-CSF) and FliC protein were expressed by pSUMO in E.coli Rosetta (DE3) and purified by Ni-NTA Sepharose, respectively. Sepharose 132-141 colony stimulating factor 2 Homo sapiens 27-33