PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3622383-2 1987 Three of these proteins were also detected by affinity chromatography on calmodulin-Sepharose. Sepharose 84-93 calmodulin Bos taurus 73-83 2834593-2 1987 In the present study, phosphodiesterase, one of the typical calmodulin-dependent functional proteins, was purified from bovine lens by DEAE-cellulose chromatography, calmodulin-Sepharose 4B chromatography and Superose 12 chromatography. Sepharose 177-186 calmodulin Bos taurus 166-176 3663598-1 1987 A calmodulin-sensitive adenylate cyclase has been purified to apparent homogeneity from bovine cerebral cortex using calmodulin-Sepharose followed by forskolin-Sepharose and wheat germ agglutinin-Sepharose. Sepharose 128-137 calmodulin Bos taurus 117-127 4041438-1 1985 A calmodulin-sensitive adenylate cyclase was purified 3000-fold from bovine cerebral cortex using DEAE-Sephacel, calmodulin-Sepharose, and two heptanediamine-Sepharose column steps. Sepharose 124-133 calmodulin Bos taurus 2-12 4074367-0 1985 Rapid purification of calmodulin and S-100 protein by affinity chromatography with melittin immobilized to sepharose. Sepharose 107-116 calmodulin Bos taurus 22-32 4074367-1 1985 Melittin-Sepharose was prepared for Ca2+-dependent affinity chromatography of calmodulin and S-100 protein. Sepharose 9-18 calmodulin Bos taurus 78-88 4074367-3 1985 Recovery of calmodulin from melittin-Sepharose was related to the degree of saturation of column capacity with lower yields when only partial saturation was achieved. Sepharose 37-46 calmodulin Bos taurus 12-22 2948561-1 1986 P-57 is a neurospecific calmodulin binding protein that was discovered by virtue of its unusual interactions with calmodulin-Sepharose [Andreasen, T. J., Luetje, C. W., Heideman, W., & Storm, D. R. (1983) Biochemistry 22, 4615-4618; Cimler, B. M., Andreasen, T. J., Andreasen, K. I., & Storm, D. R. (1985) J. Biol. Sepharose 125-134 calmodulin Bos taurus 24-34 2948561-1 1986 P-57 is a neurospecific calmodulin binding protein that was discovered by virtue of its unusual interactions with calmodulin-Sepharose [Andreasen, T. J., Luetje, C. W., Heideman, W., & Storm, D. R. (1983) Biochemistry 22, 4615-4618; Cimler, B. M., Andreasen, T. J., Andreasen, K. I., & Storm, D. R. (1985) J. Biol. Sepharose 125-134 calmodulin Bos taurus 114-124 2948561-4 1986 In contrast to other calmodulin binding proteins, P-57 has higher affinity for calmodulin-Sepharose in the absence of calcium compared to that in the presence of calcium. Sepharose 90-99 calmodulin Bos taurus 79-89 4091264-0 1985 Separation of fodrin subunits by affinity chromatography on calmodulin-Sepharose. Sepharose 71-80 calmodulin Bos taurus 60-70 4091264-3 1985 This has been exploited for separating the fodrin subunits rapidly and quantitatively by affinity chromatography on calmodulin-Sepharose. Sepharose 127-136 calmodulin Bos taurus 116-126 2994666-5 1985 The oncomodulin concentration required for the half-maximal activation of the heart phosphodiesterase was estimated to be 2 X 10(-7)M. In addition, the possibility of the observed activation by oncomodulin being due to calmodulin contamination can be ruled out as the oncomodulin activation profiles were unaltered subsequent to chromatography on organomercurial agarose and the activation by oncomodulin could not be reversed by anti-calmodulin IgG. Sepharose 363-370 calmodulin Bos taurus 219-229 4041438-1 1985 A calmodulin-sensitive adenylate cyclase was purified 3000-fold from bovine cerebral cortex using DEAE-Sephacel, calmodulin-Sepharose, and two heptanediamine-Sepharose column steps. Sepharose 158-167 calmodulin Bos taurus 2-12 6325422-1 1984 A new, rapid method for purification of calmodulin-stimulated phosphodiesterase from bovine, ovine, and porcine brain using only DEAE-agarose and calmodulin-Sepharose chromatography is described. Sepharose 157-166 calmodulin Bos taurus 40-50 3006746-6 1985 Chlorpromazine-Sepharose affinity chromatography of peptide/calmodulin adducts showed that a significant portion of the cross-linked beta-endorphin 14-31/calmodulin complex (stoichiometry of 1 mol/mol) retained the ability to interact with the immobilized phenothiazine in a Ca2+-dependent and calmodulin-displaceable manner. Sepharose 15-24 calmodulin Bos taurus 154-164 3006746-6 1985 Chlorpromazine-Sepharose affinity chromatography of peptide/calmodulin adducts showed that a significant portion of the cross-linked beta-endorphin 14-31/calmodulin complex (stoichiometry of 1 mol/mol) retained the ability to interact with the immobilized phenothiazine in a Ca2+-dependent and calmodulin-displaceable manner. Sepharose 15-24 calmodulin Bos taurus 154-164 6331319-8 1984 Formation of a Ca2+-dependent complex between calmodulin and the phosphatase was demonstrated by a calmodulin-Sepharose affinity column, gel-filtration chromatography, and sedimentation on a sucrose density gradient. Sepharose 110-119 calmodulin Bos taurus 46-56 6331319-8 1984 Formation of a Ca2+-dependent complex between calmodulin and the phosphatase was demonstrated by a calmodulin-Sepharose affinity column, gel-filtration chromatography, and sedimentation on a sucrose density gradient. Sepharose 110-119 calmodulin Bos taurus 99-109 6685738-1 1983 Calmodulin was purified from bovine testis and soybean seed, using affinity chromatography on (6-amino-hexyl)-5-chloro-1-naphthylene sulfonamide (W7)-Sepharose. Sepharose 150-159 calmodulin Bos taurus 0-10 16593360-5 1983 Calmodulin-depleted fractions containing quinate:NAD(+) oxidoreductase were obtained by passage of the crude extracts through an affinity column of 2-chloro-10-(3-aminopropyl)phenothiazine coupled to Sepharose 4B. Sepharose 200-209 calmodulin Bos taurus 0-10 6313045-3 1983 The protein was purified by DEAE-Sephacel chromatography and two CaM-Sepharose affinity column steps. Sepharose 69-78 calmodulin Bos taurus 65-68 6313045-4 1983 The first CaM-Sepharose column was run in the presence of Ca2+; the second was run in the presence of chelator in excess of Ca2+. Sepharose 14-23 calmodulin Bos taurus 10-13 6296146-2 1983 The majority of protein in a bovine brain extract that binds to a calmodulin-Sepharose affinity column also is observed to bind in a metal ion-independent manner to phenyl-Sepharose through hydrophobic interactions. Sepharose 77-86 calmodulin Bos taurus 66-76 6296146-6 1983 Comparison of the proteins which are bound to and eluted from phenyl- and calmodulin-Sepharose affinity columns indicates that chromatography involving calmodulin-Sepharose resembles hydrophobic interaction chromatography with charged ligands. Sepharose 85-94 calmodulin Bos taurus 74-84 6296146-6 1983 Comparison of the proteins which are bound to and eluted from phenyl- and calmodulin-Sepharose affinity columns indicates that chromatography involving calmodulin-Sepharose resembles hydrophobic interaction chromatography with charged ligands. Sepharose 85-94 calmodulin Bos taurus 152-162 6296146-6 1983 Comparison of the proteins which are bound to and eluted from phenyl- and calmodulin-Sepharose affinity columns indicates that chromatography involving calmodulin-Sepharose resembles hydrophobic interaction chromatography with charged ligands. Sepharose 163-172 calmodulin Bos taurus 74-84 6296146-6 1983 Comparison of the proteins which are bound to and eluted from phenyl- and calmodulin-Sepharose affinity columns indicates that chromatography involving calmodulin-Sepharose resembles hydrophobic interaction chromatography with charged ligands. Sepharose 163-172 calmodulin Bos taurus 152-162 6685738-3 1983 Although bovine and soybean calmodulins share affinity for W7-Sepharose, share biological activity, and have similar amino acid compositions, antisera raised against soybean calmodulin recognize animal calmodulins with 20,000-fold less avidity than they do plant calmodulins. Sepharose 62-71 calmodulin Bos taurus 28-38 6267040-1 1981 Myosin light chain kinase and a fraction of type II cAMP-dependent protein kinase have been partially purified from bovine brain by affinity chromatography on calmodulin-Sepharose. Sepharose 170-179 calmodulin Bos taurus 159-169 7085752-6 1982 The ciliary protein is identified as a calmodulin on the basis of its calcium- dependent binding to a fluphenazine-sepharose affinity column and its comigration with bovine brain calmodulin on alkaline-urea and SDS polyacrylamide gels in both the presence and absence of calcium. Sepharose 115-124 calmodulin Bos taurus 39-49 6947252-2 1981 Bovine brain calmodulin, rabbit skeletal muscle troponin C, and bovine brain S100b bind to phenothiazine-Sepharose in a calcium-dependent manner. Sepharose 105-114 calmodulin Bos taurus 13-58 6896820-9 1982 Myosin kinase II activity was completely Ca(2+)-dependent after affinity chromatography on the calmodulin-Sepharose column. Sepharose 106-115 calmodulin Bos taurus 95-105 6267040-3 1981 A fraction of soluble cAMP-dependent protein kinase also bound to calmodulin-Sepharose and was purified 2300-fold. Sepharose 77-86 calmodulin Bos taurus 66-76 6247353-1 1980 Calmodulin-dependent cyclic nucleotide phosphodiesterase was purified from bovine brain to apparent homogeneity by a new procedure involving DEAE-cellulose, Affi-Gel blue, calmodulin-Sepharose 4B, and Sephadex G-200 column chromatographies. Sepharose 183-192 calmodulin Bos taurus 0-10 6894553-2 1981 It was purified 130-fold with a 10% yield by anion-exchange chromatography followed by affinity chromatography on calmodulin-Sepharose. Sepharose 125-134 calmodulin Bos taurus 114-124 6257728-1 1980 Calmodulin has been purified from cell bodies of the green alga Chlamydomonas by Ca++-dependent affinity chromatography on fluphenazine-Sepharose 4B. Sepharose 136-145 calmodulin Bos taurus 0-10 6448862-12 1980 The presence of Ca++-dependent calmodulin-binding sites on 14S and 30S dyneins was demonstrated by the Ca++-dependent retention of the dyneins on a calmodulin-Sepharose-4B column. Sepharose 159-168 calmodulin Bos taurus 31-41 6448862-12 1980 The presence of Ca++-dependent calmodulin-binding sites on 14S and 30S dyneins was demonstrated by the Ca++-dependent retention of the dyneins on a calmodulin-Sepharose-4B column. Sepharose 159-168 calmodulin Bos taurus 148-158 16592801-6 1980 The calmodulin synthesized in vitro has been isolated by using calcium-dependent affinity chromatography on phenothiazine-Sepharose conjugates. Sepharose 122-131 calmodulin Bos taurus 4-14 2295605-2 1990 The purification procedure involved DEAE-Sepharose CL-6B (to remove calmodulin), calmodulin-Sepharose 4B affinity, and Sepharose 6B column chromatographies. Sepharose 92-101 calmodulin Bos taurus 81-91 17131818-0 2006 [Calcium-dependent interaction of transducin with calmodulin-sepharose]. Sepharose 61-70 calmodulin Bos taurus 50-60 17131818-1 2006 It has been shown by affinity chromatography on calmodulin-sepharose that transducin, a G protein of bovine retinal rod outer segments interacts with Ca(2+)-calmodulin. Sepharose 59-68 calmodulin Bos taurus 48-58 17131818-1 2006 It has been shown by affinity chromatography on calmodulin-sepharose that transducin, a G protein of bovine retinal rod outer segments interacts with Ca(2+)-calmodulin. Sepharose 59-68 calmodulin Bos taurus 157-167 7532400-9 1995 This enzyme could be partially purified from epimastigote plasma-membrane vesicles using calmodulin-agarose affinity chromatography. Sepharose 100-107 calmodulin Bos taurus 89-99 7836475-2 1995 The binding of clathrin-coated vesicles, clathrin triskelions, and free clathrin light chains to calmodulin-Sepharose was compared. Sepharose 108-117 calmodulin Bos taurus 97-107 7836475-3 1995 When isolated from bovine brain, all three components bound to calmodulin-Sepharose in the presence of calcium and could be eluted by its removal. Sepharose 74-83 calmodulin Bos taurus 63-73 7836475-7 1995 Recombinant mutants of LCa, with deletions spanning the entire sequence, were tested for binding to calmodulin-Sepharose. Sepharose 111-120 calmodulin Bos taurus 100-110 8366104-6 1993 The calmodulin binding domain in the petunia GAD was mapped by binding truncated forms of GAD immobilized on nitrocellulose membranes to recombinant petunia 35S-calmodulin as well as to biotinylated bovine calmodulin and by binding truncated forms of GAD to calmodulin-Sepharose columns. Sepharose 269-278 calmodulin Bos taurus 4-14 1590758-1 1992 The interactions between guanine nucleotide regulatory proteins and the Ca(2+)-binding protein calmodulin were studied using calmodulin-Sepharose affinity chromatography. Sepharose 136-145 calmodulin Bos taurus 95-105 1590758-1 1992 The interactions between guanine nucleotide regulatory proteins and the Ca(2+)-binding protein calmodulin were studied using calmodulin-Sepharose affinity chromatography. Sepharose 136-145 calmodulin Bos taurus 125-135 1590758-2 1992 Purified bovine brain beta gamma subunits bound to calmodulin-Sepharose in a Ca(2+)-dependent manner. Sepharose 62-71 calmodulin Bos taurus 51-61 1590758-6 1992 In contrast to bovine brain G proteins, both purified transducin beta gamma subunits and beta gamma released from rhodopsin-activated transducin bound to calmodulin-Sepharose in a Ca(2+)-dependent manner. Sepharose 165-174 calmodulin Bos taurus 154-164 1655737-9 1991 An active recombinant catalytic subunit was expressed in bacteria and purified by CaM-Sepharose chromatography. Sepharose 86-95 calmodulin Bos taurus 82-85 11311128-4 2001 In the present paper we report the direct interaction of TRP4 and calmodulin (CaM) by: (1) retention of in vitro translated TRP4 and of TRP4 protein solubilized from bovine adrenal cortex by CaM-Sepharose in the presence of Ca(2+), and (2) TRP4-glutathione S-transferase pull-down experiments. Sepharose 195-204 calmodulin Bos taurus 66-76 11311128-4 2001 In the present paper we report the direct interaction of TRP4 and calmodulin (CaM) by: (1) retention of in vitro translated TRP4 and of TRP4 protein solubilized from bovine adrenal cortex by CaM-Sepharose in the presence of Ca(2+), and (2) TRP4-glutathione S-transferase pull-down experiments. Sepharose 195-204 calmodulin Bos taurus 78-81 2026258-2 1991 This method takes advantage of the fact that the protein remains soluble in 2.5% perchloric acid (PCA) and that it binds to a calmodulin-Sepharose column in the absence of calcium: Other PKC substrate proteins that remain to be identified were also found to share these two properties, suggesting that a class of calmodulin-binding PKC substrates may exist in the brain. Sepharose 137-146 calmodulin Bos taurus 126-136 2835007-7 1988 The calmodulin-stimulated phosphodiesterase activity in the sperm plasma membranes can be solubilized and absorbed to a Calmodulin-Sepharose affinity column in the presence of Ca2+. Sepharose 131-140 calmodulin Bos taurus 4-14 2835007-7 1988 The calmodulin-stimulated phosphodiesterase activity in the sperm plasma membranes can be solubilized and absorbed to a Calmodulin-Sepharose affinity column in the presence of Ca2+. Sepharose 131-140 calmodulin Bos taurus 120-130 3338465-7 1988 The two adenylyl cyclases from brain can be separated by calmodulin-Sepharose: only the enzyme of 115 kDa but not that of 150 kDa was retained by the affinity resin and could be stimulated by Ca2+/calmodulin. Sepharose 68-77 calmodulin Bos taurus 57-67 3338465-7 1988 The two adenylyl cyclases from brain can be separated by calmodulin-Sepharose: only the enzyme of 115 kDa but not that of 150 kDa was retained by the affinity resin and could be stimulated by Ca2+/calmodulin. Sepharose 68-77 calmodulin Bos taurus 197-207