PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
2422454-0	1986	Affinity chromatography of rat alpha-fetoprotein on estradiol-agarose columns.	Sepharose	62-69	alpha-fetoprotein	Rattus norvegicus	31-48	
2422454-1	1986	The use of 17 beta-estradiol-17-hemisuccinate coupled to agarose beads is shown to be a rapid and simple procedure for the isolation of alpha-fetoprotein (AFP) from amniotic fluid.	Sepharose	57-64	alpha-fetoprotein	Rattus norvegicus	136-153	
2422454-1	1986	The use of 17 beta-estradiol-17-hemisuccinate coupled to agarose beads is shown to be a rapid and simple procedure for the isolation of alpha-fetoprotein (AFP) from amniotic fluid.	Sepharose	57-64	alpha-fetoprotein	Rattus norvegicus	155-158	
6197305-1	1983	By affinity chromatography on concanavalin A (ConA) linked to Sepharose, S-carboxymethylated rat alpha-fetoprotein could be separated into two molecular variants: a ConA-reactive and a ConA-nonreactive fraction.	Sepharose	62-71	alpha-fetoprotein	Rattus norvegicus	97-114	
6208454-1	1984	Two structural variants of the rat alpha-fetoprotein (AFP) gene have been detected in different inbred strains of rats by EcoRI or HindIII restriction enzyme cleavage of cellular DNA, agarose gel electrophoresis and Southern blot hybridization using 32P-labeled cloned rat AFP cDNA probes.	Sepharose	184-191	alpha-fetoprotein	Rattus norvegicus	35-52	
6208454-1	1984	Two structural variants of the rat alpha-fetoprotein (AFP) gene have been detected in different inbred strains of rats by EcoRI or HindIII restriction enzyme cleavage of cellular DNA, agarose gel electrophoresis and Southern blot hybridization using 32P-labeled cloned rat AFP cDNA probes.	Sepharose	184-191	alpha-fetoprotein	Rattus norvegicus	54-57	
87217-4	1979	The albumin mRNA possessed a chain length of approximately 2265 nucleotides and the AFP mRNA possesed a length of approximately 2235 nucleotides when examined under stringent denaturing conditions on agarose gels containing 10 mM methylmercury hydroxide.	Sepharose	200-207	alpha-fetoprotein	Rattus norvegicus	84-87	
6194262-1	1983	Monospecific anti-rat serum alpha-fetoprotein (AFP) IgG was coupled to cyanogen bromide-activated Sepharose-4B (4.5 mg/ml packed volume of gel) to yield an immunoaffinity matrix.	Sepharose	98-110	alpha-fetoprotein	Rattus norvegicus	47-50	
70227-8	1977	The slow moving alpha-fetoprotein could be further fractionated on RCAI-sepharose column in two components, AFPA1 and AFPA2 differing by their sialic acid content.	Sepharose	72-81	alpha-fetoprotein	Rattus norvegicus	16-33	
47266-4	1975	The presence of AFP in the serum was determined by double diffusion in agarose and by comparison also by quantitative radioimmunoassay.	Sepharose	71-78	alpha-fetoprotein	Rattus norvegicus	16-19	
57803-1	1976	Two variants of alpha-fetoprotein in rat amniotic fluid were separated by their different affinity for concanavalin A-Sepharose, which selectively binds alpha-D-manno-pyranosides and alpha-D-glucopyranosides.	Sepharose	118-127	alpha-fetoprotein	Rattus norvegicus	16-33