PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 28536031-4 2017 This osteoblast differentiation-inducing effect of lactate can be inhibited by blocking its entry into cells with MCT1 siRNA or inhibitors, and by interfering with its metabolism by using specific siRNAs for LDHB and PDH. Lactic Acid 51-58 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 217-220 27144334-3 2016 Reduced pyruvate metabolism via PDH could lead to increased pyruvate conversion to lactate. Lactic Acid 83-90 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 32-35 27179453-0 2016 Metabolic reprogramming by the pyruvate dehydrogenase kinase-lactic acid axis: Linking metabolism and diverse neuropathophysiologies. Lactic Acid 61-72 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 31-53 27179453-2 2016 In particular, the pyruvate dehydrogenase (PDH) kinase (PDK)-lactic acid axis is a critical link that connects metabolic reprogramming and the pathophysiology of neurological disorders. Lactic Acid 61-72 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 19-41 27179453-2 2016 In particular, the pyruvate dehydrogenase (PDH) kinase (PDK)-lactic acid axis is a critical link that connects metabolic reprogramming and the pathophysiology of neurological disorders. Lactic Acid 61-72 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 43-46 27179453-3 2016 PDKs, via regulation of PDH complex activity, orchestrate the conversion of pyruvate either aerobically to acetyl-CoA, or anaerobically to lactate. Lactic Acid 139-146 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 24-27 27221120-6 2016 Changes in insulin-stimulated PDH phosphorylation were positively related to changes in plasma lactate. Lactic Acid 95-102 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 30-33 27221120-8 2016 Twelve weeks of endurance- or strength-oriented exercise training improved insulin-stimulated PDH dephosphorylation, which was related to a reduced lactate response. Lactic Acid 148-155 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 94-97 21346867-2 2010 The activation of PDH leads to a reduction in ambient cellular lactate concentrations both in vitro and in vivo which contributes to the therapeutic use of DCA in the treatment of systemic lactic acidosis in humans. Lactic Acid 63-70 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 18-21 24452394-10 2014 The metabolic impact of PDH reactivation was determined by glucose and lactate assays. Lactic Acid 71-78 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 24-27 22508045-2 2012 Dichloroacetate (DCA) is a drug which is able to shift pyruvate metabolism from lactate to acetyl-CoA (tricarboxylic acid cycle) by indirect activation of pyruvate dehydrogenase (PDH). Lactic Acid 80-87 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 155-177 22508045-2 2012 Dichloroacetate (DCA) is a drug which is able to shift pyruvate metabolism from lactate to acetyl-CoA (tricarboxylic acid cycle) by indirect activation of pyruvate dehydrogenase (PDH). Lactic Acid 80-87 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 179-182 25046652-9 2015 Hyperpolarized [1-(13) C]lactate may enable noninvasive assessment of cardiac PDH flux in cardiac patients in the near future. Lactic Acid 25-32 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 78-81 16469933-8 2006 The active form of PDH was higher after training (main effect, P=0.04), and net muscle glycogenolysis (posttraining: 100+/-16 vs. pretraining: 139+/-11 mmol/kg dry wt; P=0.03) and lactate accumulation (posttraining: 55+/-2 vs. pretraining: 63+/-1 mmol/kg dry wt; P=0.03) during exercise were reduced. Lactic Acid 180-187 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 19-22 17521432-4 2007 The two key enzymatiques systems required for the production of these monocarboxylates are lactate dehydrogenase (LDH; EC1.1.1.27) that catalyses the interconversion of lactate and pyruvate and the pyruvate dehydrogenase complex that irreversibly funnels pyruvate towards the mitochondrial TCA and oxydative phosphorylation. Lactic Acid 91-98 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 198-220 17384007-1 2007 BACKGROUND: Although the blood lactate-to-pyruvate (L:P) molar ratio is used to distinguish between pyruvate dehydrogenase deficiency (PDH-D) and other causes of congenital lactic acidosis (CLA), its diagnostic accuracy for differentiating between these 2 types of CLA has not been evaluated formally. Lactic Acid 31-38 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 135-138 17384007-4 2007 We observed a strong positive association between L:P ratio and blood lactate in non-PDH CLA, whereas this association was weak in PDH-D CLA. Lactic Acid 50-51 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 85-88 17384007-4 2007 We observed a strong positive association between L:P ratio and blood lactate in non-PDH CLA, whereas this association was weak in PDH-D CLA. Lactic Acid 70-77 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 85-88 10649979-6 2000 (+)-1-N-[2,5-(S, R)-Dimethyl-4-N-(4-cyanobenzoyl)piperazine]-(R)-3,3, 3-trifluoro-2-hydroxy-2-methylpropanamide (14e) inhibits PDHK in the primary enzymatic assay with an IC(50) of 16 +/- 2 nM, enhances the oxidation of [(14)C]lactate into (14)CO(2) in human fibroblasts with an EC(50) of 57 +/- 13 nM, diminishes lactate significantly 2.5 h post-oral-dose at doses as low as 1 micromol/kg, and increases the ex vivo activity of PDH in muscle, liver, and fat tissues in normal Sprague-Dawley rats. Lactic Acid 227-234 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 127-130 10776894-5 2000 RESULTS: At low power outputs, the rates of pyruvate and NADH production in the cytoplasm are low, and pyruvate dehydrogenase (PDH) and the shuttle system enzymes (SS) metabolize the majority of these substrates, resulting in little or no lactate production. Lactic Acid 239-246 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 103-125 10710508-12 2000 Hence, the elevated lactate production associated with hypoxia at the same absolute workload could in part be explained by the effects of hypoxia on the activities of the rate-limiting enzymes, phosphorylase and PDH, which regulate the rates of pyruvate production and pyruvate oxidation, respectively. Lactic Acid 20-27 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 212-215 11001755-1 2000 During the onset of exercise in hypoxia, the increased lactate accumulation is associated with a delayed activation of pyruvate dehydrogenase (PDH; Parolin ML, Spreit LL, Hultman E, Hollidge-Horvat MG, Jones NL, and Heigenhauser GJF. Lactic Acid 55-62 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 143-146 11001755-3 2000 The present study investigated whether activation of PDH with dichloroacetate (DCA) before exercise would reduce lactate accumulation during exercise in acute hypoxia by increasing oxidative phosphorylation. Lactic Acid 113-120 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 53-56 10826498-17 2000 Enhanced pyruvate flux through PDH is beneficial for the cardiac cells since less pyruvate is converted to lactate, a metabolic step resulting in the acidification of the intracellular compartment. Lactic Acid 107-114 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 31-34 10649979-6 2000 (+)-1-N-[2,5-(S, R)-Dimethyl-4-N-(4-cyanobenzoyl)piperazine]-(R)-3,3, 3-trifluoro-2-hydroxy-2-methylpropanamide (14e) inhibits PDHK in the primary enzymatic assay with an IC(50) of 16 +/- 2 nM, enhances the oxidation of [(14)C]lactate into (14)CO(2) in human fibroblasts with an EC(50) of 57 +/- 13 nM, diminishes lactate significantly 2.5 h post-oral-dose at doses as low as 1 micromol/kg, and increases the ex vivo activity of PDH in muscle, liver, and fat tissues in normal Sprague-Dawley rats. Lactic Acid 314-321 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 127-130 10567017-10 1999 Lactate accumulation appeared to be due to an imbalance of the relative activities of Phos and PDH. Lactic Acid 0-7 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 95-98 10567017-11 1999 The increase in H(+) concentration may have served to reduce pyruvate production by inhibiting Phos transformation and may have simultaneously activated PDH in the third bout such that there was a better matching between pyruvate production and oxidation and minimal lactate accumulation. Lactic Acid 267-274 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 153-156 8142598-3 1993 Furthermore, maximal stimulation of pyruvate dehydrogenase (PDH) by dichloroacetate (DCA) treatment in conjunction with cachectin/TNF abolished lactate production, but increased glucose uptake persisted. Lactic Acid 144-151 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 36-58 10486093-3 1999 PDH deficiency was diagnosed in adolescence and both girls had low PDH complex activity in muscle but normal amounts of all subunits on Western blotting, and a normal lactate/pyruvate ratio in blood and CSF. Lactic Acid 167-174 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 0-3 9555066-2 1998 During reperfusion, PDH activities were suppressed corresponding to the poor recovery of ATP and PCr concentrations and the increase in lactate concentration in the hyperglycemic group, suggesting that preischemic hyperglycemia may impair metabolism by suppressing PDH activity. Lactic Acid 136-143 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 20-23 9546635-7 1998 Furthermore, oxidation of carbohydrates (glucose, pyruvate, and lactate) was greatly increased in new born myocytes compared to 2 week and HY cells and was accompanied by a parallel increase in pyruvate dehydrogenase (PDH) activity. Lactic Acid 64-71 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 194-216 8598634-8 1995 We conclude that PCR-SSCP analysis of the PDH E1 alpha gene, followed by DNA sequencing, is a useful method to screen for mutations of the PDH E1 alpha gene in female patients with congenital lactic acidaemia who have normal enzyme activities in available samples, normal ratio of lactate to pyruvate, and predominantly raised lactate concentration in cerebrospinal fluid. Lactic Acid 281-288 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 42-45 8598634-8 1995 We conclude that PCR-SSCP analysis of the PDH E1 alpha gene, followed by DNA sequencing, is a useful method to screen for mutations of the PDH E1 alpha gene in female patients with congenital lactic acidaemia who have normal enzyme activities in available samples, normal ratio of lactate to pyruvate, and predominantly raised lactate concentration in cerebrospinal fluid. Lactic Acid 281-288 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 139-142 8598634-8 1995 We conclude that PCR-SSCP analysis of the PDH E1 alpha gene, followed by DNA sequencing, is a useful method to screen for mutations of the PDH E1 alpha gene in female patients with congenital lactic acidaemia who have normal enzyme activities in available samples, normal ratio of lactate to pyruvate, and predominantly raised lactate concentration in cerebrospinal fluid. Lactic Acid 327-334 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 42-45 8598634-8 1995 We conclude that PCR-SSCP analysis of the PDH E1 alpha gene, followed by DNA sequencing, is a useful method to screen for mutations of the PDH E1 alpha gene in female patients with congenital lactic acidaemia who have normal enzyme activities in available samples, normal ratio of lactate to pyruvate, and predominantly raised lactate concentration in cerebrospinal fluid. Lactic Acid 327-334 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 139-142 8076623-1 1994 We investigated the effects of a stimulation of pyruvate dehydrogenase (PDH) activity induced by 2-chloropropionate (2-CP) on venous plasma lactate concentration and peak anaerobic power (W an, peak) during periods (6 s) of incremental intense exercise, i.e. a force-velocity (F-v) test known to induce a marked accumulation of lactate in the blood. Lactic Acid 140-147 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 72-75 8076623-1 1994 We investigated the effects of a stimulation of pyruvate dehydrogenase (PDH) activity induced by 2-chloropropionate (2-CP) on venous plasma lactate concentration and peak anaerobic power (W an, peak) during periods (6 s) of incremental intense exercise, i.e. a force-velocity (F-v) test known to induce a marked accumulation of lactate in the blood. Lactic Acid 328-335 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 72-75 8076623-9 1994 In conclusion, PDH activation by 2-CP attenuated the increase in venous plasma lactate concentration during the F-v test. Lactic Acid 79-86 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 15-18 9818465-11 1998 A significant increase of the lactate level by more than 1 mmol/l during an oral glucose load was found in 11 of 16 children with impairment of the respiratory chain or PDH complex. Lactic Acid 30-37 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 169-172 9688884-1 1998 Muscle metabolism, including the role of pyruvate dehydrogenase (PDH) in muscle lactate (Lac-) production, was examined during incremental exercise before and after 7 days of submaximal training on a cycle ergometer [2 h daily at 60% peak O2 uptake (VO2 max)]. Lactic Acid 80-87 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 41-63 9688884-1 1998 Muscle metabolism, including the role of pyruvate dehydrogenase (PDH) in muscle lactate (Lac-) production, was examined during incremental exercise before and after 7 days of submaximal training on a cycle ergometer [2 h daily at 60% peak O2 uptake (VO2 max)]. Lactic Acid 80-87 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 65-68 8142598-3 1993 Furthermore, maximal stimulation of pyruvate dehydrogenase (PDH) by dichloroacetate (DCA) treatment in conjunction with cachectin/TNF abolished lactate production, but increased glucose uptake persisted. Lactic Acid 144-151 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 60-63 1600837-4 1992 Stimulation of PDH by DCA increases peripheral oxidation of alanine and lactate, thereby interrupting the Cori and alanine cycles and reducing the availability of three-carbon precursors for gluconeogenesis. Lactic Acid 72-79 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 15-18 1736740-2 1992 Although elevated lactate levels are generally assumed to be a marker of anaerobiosis in these patients, endotoxin may increase serum lactate by inactivation of pyruvate dehydrogenase (PDH), unrelated to tissue PO2. Lactic Acid 134-141 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 161-183 1736740-2 1992 Although elevated lactate levels are generally assumed to be a marker of anaerobiosis in these patients, endotoxin may increase serum lactate by inactivation of pyruvate dehydrogenase (PDH), unrelated to tissue PO2. Lactic Acid 134-141 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 185-188 1736740-10 1992 These data show that lactate levels in endotoxic states may be a poor marker of tissue hypoxia and may be more related to PDH activity. Lactic Acid 21-28 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 122-125 31792175-5 2019 We have shown that human peritoneal mesothelial cells (HPMCs) recovered from the pelvic peritoneum of women with endometriosis exhibit significantly higher glycolysis, lower mitochondrial respiration, decreased enzymatic activity of pyruvate dehydrogenase (PDH), and increased production of lactate compared to HPMCs from women without disease. Lactic Acid 291-298 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 233-255 3956111-5 1986 After exercise muscle glycogen fell to the greatest extent and lactate rose the least in the post-training state, with opposite findings post-immobilization, suggesting that PDH activation contributes to the control of lactate formation in muscle during heavy exercise, and that the effects of training and immobilization are mediated at least in part through changes in the activation of this regulatory enzyme. Lactic Acid 219-226 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 174-177 33675030-4 2022 Activation of PDK suppresses the conversion of pyruvate to acetyl-coenzyme A (acetyl-CoA) through the inactivation of PDH, which allows the cumulus cells to supply sufficient amounts of pyruvate, lactate, and nicotinamide adenine dinucleotide phosphate (NADPH) to the oocytes. Lactic Acid 196-203 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 118-121 32998265-7 2020 In human OSCC cells, lactate secretion decreased when lactate fermentation was suppressed by knockdown of ME1 and lactate dehydrogenase A or inhibition of pyruvate dehydrogenase (PDH) kinase. Lactic Acid 21-28 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 155-177 32998265-7 2020 In human OSCC cells, lactate secretion decreased when lactate fermentation was suppressed by knockdown of ME1 and lactate dehydrogenase A or inhibition of pyruvate dehydrogenase (PDH) kinase. Lactic Acid 21-28 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 179-182 32998265-7 2020 In human OSCC cells, lactate secretion decreased when lactate fermentation was suppressed by knockdown of ME1 and lactate dehydrogenase A or inhibition of pyruvate dehydrogenase (PDH) kinase. Lactic Acid 54-61 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 155-177 32998265-7 2020 In human OSCC cells, lactate secretion decreased when lactate fermentation was suppressed by knockdown of ME1 and lactate dehydrogenase A or inhibition of pyruvate dehydrogenase (PDH) kinase. Lactic Acid 54-61 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 179-182 32998265-9 2020 In contrast, when oxidative phosphorylation was suppressed by PDH knockdown, lactate secretion increased, extracellular pH decreased, and the EMT phenotype was promoted. Lactic Acid 77-84 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 62-65 32433492-5 2020 Fibroblasts cultured in lactate-only medium exhibited reduced PDH phosphorylation, indicative of OXPHOS, and a concurrent elevation of ROS. Lactic Acid 24-31 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 62-65 31828927-4 2020 PDK1 is a key enzyme in the transition of glycolysis to tricarboxylic acid cycle, via inactivating PDH and converting oxidative phosphorylation to Warburg effect, resulting in increment of lactate production. Lactic Acid 189-196 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 99-102 2751069-2 1989 During therapy, the rates of decarboxylation of (1-14C) pyruvate and (3-14C) pyruvate, which represent the activity of the pyruvate dehydrogenase (PDH) complex and the function of the TCA cycle, respectively, were markedly increased in the platelets and increases in the lactate levels in the blood and urine during exercise were markedly reduced. Lactic Acid 271-278 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 123-145 2751069-2 1989 During therapy, the rates of decarboxylation of (1-14C) pyruvate and (3-14C) pyruvate, which represent the activity of the pyruvate dehydrogenase (PDH) complex and the function of the TCA cycle, respectively, were markedly increased in the platelets and increases in the lactate levels in the blood and urine during exercise were markedly reduced. Lactic Acid 271-278 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 147-150 2751069-3 1989 These results suggest that oral administration of DCA causes significant increases in the activities of the PDH complex and TCA cycle not only in the platelets but also in various tissues of humans, which is important as a pathway for production of energy, resulting in decreases in the lactate and pyruvate levels in the blood and cerebrospinal fluid. Lactic Acid 287-294 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 108-111 31792175-7 2019 Treatment of endometriosis HPMCs with the pyruvate dehydrogenase kinase (PDK) inhibitor/PDH activator dichloroacetate (DCA) normalizes HPMC metabolism, reduces lactate secretion, and abrogates endometrial stromal cell proliferation in a coculture model. Lactic Acid 160-167 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 88-91 29444744-5 2019 The results demonstrated that overexpression of the PDHA1 gene inhibited aerobic glycolysis with lower lactate via increased PDH activity; meanwhile, mitochondrial OXPHOS was enhanced accompanied with higher ATP and lower glucose consumption. Lactic Acid 103-110 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 52-55 30923124-5 2019 We also observed that LDHB knockdown increases inhibitory phosphorylation of pyruvate dehydrogenase (PDH) via lactate-mediated PDH kinase (PDK) activation and thereby attenuates oxidative phosphorylation activity. Lactic Acid 110-117 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 77-99 30923124-5 2019 We also observed that LDHB knockdown increases inhibitory phosphorylation of pyruvate dehydrogenase (PDH) via lactate-mediated PDH kinase (PDK) activation and thereby attenuates oxidative phosphorylation activity. Lactic Acid 110-117 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 101-104 30923124-6 2019 Interestingly, monocarboxylate transporter 1 was the major lactate transporter in hepatoma cells, and its expression was essential for PDH phosphorylation by modulating intracellular lactate levels. Lactic Acid 59-66 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 135-138 29615918-7 2018 Thirty minutes after the sprint Ser293-PDH-E1alpha phosphorylation was greater with antioxidants, resulting in 74% higher muscle lactate concentration. Lactic Acid 129-136 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 39-42 28846077-9 2017 Incubation of MRC5 fibroblasts with lactate resulted in an increase of LDHB and of PDH expression. Lactic Acid 36-43 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 83-86