PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20534731-0 2010 Impact of differential P450c17 phosphorylation by cAMP stimulation and by starvation conditions on enzyme activities and androgen production in NCI-H295R cells. Cyclic AMP 50-54 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 23-30 20534731-10 2010 Time-course experiments revealed that a short cAMP stimulation augmented threonine phosphorylation of P450c17 but did not increase 17,20-lyase activity. Cyclic AMP 46-50 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 102-109 20534731-11 2010 By contrast, long cAMP stimulation increased androgen production through increased P450c17 activities by enhancing CYP17A1 gene expression. Cyclic AMP 18-22 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 83-90 20534731-11 2010 By contrast, long cAMP stimulation increased androgen production through increased P450c17 activities by enhancing CYP17A1 gene expression. Cyclic AMP 18-22 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 115-122 20534731-13 2010 In addition, our study shows that starvation and cAMP stimulation regulate P450c17 phosphorylation differentially and that an increase in P450c17 phosphorylation does not necessarily lead to enhanced enzyme activity and androgen production. Cyclic AMP 49-53 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 75-82 20615422-6 2010 Exposure to 8:2 FTOH significantly down-regulated cAMP-dependent mRNA expression and protein abundance of several key steroidogenic enzymes, including StAR, CYP11A, CYP11B1, CYP11B2, CYP17 and CYP21. Cyclic AMP 50-54 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 183-188 20534731-4 2010 cAMP increases CYP17A1 expression, P450c17 phosphorylation, and androgen production. Cyclic AMP 0-4 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 15-22 20534731-4 2010 cAMP increases CYP17A1 expression, P450c17 phosphorylation, and androgen production. Cyclic AMP 0-4 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 35-42 20160131-4 2010 One factor favoring more efficient electron transfer and 17,20 lyase activity is cAMP-dependent serine/threonine phosphorylation of P450c17. Cyclic AMP 81-85 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 132-139 19359379-4 2009 The introduction of LRH-1 into human MSCs (hMSCs) with the aid of cAMP also induced the expression of steroidogenic enzymes, including CYP17, and their differentiation into steroid hormone-producing cells. Cyclic AMP 66-70 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 135-140 19359379-7 2009 The CYP17 promoter region was highly methylated in hMSCs, whereas it was demethylated by the introduction of LRH-1 and cAMP treatment. Cyclic AMP 119-123 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 4-9 12506119-0 2003 CAMP-dependent protein kinase enhances CYP17 transcription via MKP-1 activation in H295R human adrenocortical cells. Cyclic AMP 0-4 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 39-44 17786540-2 2008 It has been reported that transforming growth factor beta (TGF-beta) decreases both basal and cAMP-stimulated levels of CYP17 mRNA, but the mechanism of TGF-beta action on CYP17 expression remains unknown. Cyclic AMP 94-98 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 120-125 17664281-7 2007 Inhibition of DGK activity attenuates the binding of SF1 to the CYP17 promoter, and silencing of DGK-theta expression inhibits cAMP-dependent CYP17 transcription. Cyclic AMP 127-131 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 142-147 17664281-9 2007 We conclude that ACTH/cAMP stimulates PA production in the nucleus of H295R cells and that this increase in PA concentrations facilitates CYP17 induction. Cyclic AMP 22-26 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 138-143 17121866-0 2007 Coregulator exchange and sphingosine-sensitive cooperativity of steroidogenic factor-1, general control nonderepressed 5, p54, and p160 coactivators regulate cyclic adenosine 3",5"-monophosphate-dependent cytochrome P450c17 transcription rate. Cyclic AMP 158-194 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 216-223 16981135-8 2006 DKK3 decreased cyclic AMP-stimulated CYP17. Cyclic AMP 15-25 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 37-42 16306078-1 2006 In the human adrenal cortex, ACTH activates steroid hormone biosynthesis by acutely increasing cholesterol delivery to the mitochondrion and chronically increasing the transcription of steroidogenic genes (including CYP17) via a cAMP-dependent pathway. Cyclic AMP 229-233 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 216-221 15072559-7 2004 Correlating with these data, TReP-132 increases P450c17 activities via the induction of transcript levels and promoter activity of the P450c17 gene, an effect that is enhanced in the presence of cAMP or SF-1. Cyclic AMP 195-199 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 48-55 15072559-7 2004 Correlating with these data, TReP-132 increases P450c17 activities via the induction of transcript levels and promoter activity of the P450c17 gene, an effect that is enhanced in the presence of cAMP or SF-1. Cyclic AMP 195-199 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 135-142 12782406-5 2003 Coexpression of RIP140 and SF-1 in different cell types demonstrated that RIP140 acts as a potent corepressor of transcription from the SF-1 responsive cAMP regulatory sequence 2 (CRS2) element of the CYP17 gene and a variety of SF-1 responsive promoter genes. Cyclic AMP 152-156 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 201-206 17664281-0 2007 Cyclic AMP-stimulated interaction between steroidogenic factor 1 and diacylglycerol kinase theta facilitates induction of CYP17. Cyclic AMP 0-10 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 122-127 17664281-2 2007 We recently found that sphingosine is an antagonist for SF1 and inhibits cyclic AMP (cAMP)-dependent CYP17 gene transcription. Cyclic AMP 73-83 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 101-106 17664281-2 2007 We recently found that sphingosine is an antagonist for SF1 and inhibits cyclic AMP (cAMP)-dependent CYP17 gene transcription. Cyclic AMP 85-89 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 101-106 16887917-3 2006 In the present study, our aim was to identify endogenous ligands for SF1 and characterize their functional significance in mediating cAMP-dependent transcription of human CYP17. Cyclic AMP 133-137 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 171-176 16887917-6 2006 SPH antagonized the ability of cAMP and the coactivator steroid receptor coactivator-1 to increase CYP17 reporter gene activity. Cyclic AMP 31-35 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 99-104 14988427-2 2004 Expression of the gene for P450c17 is cAMP dependent, tissue specific, developmentally programmed, and varies among species. Cyclic AMP 38-42 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 27-34 12506119-5 2003 In transient-transfection studies, transcriptional activity of an hCYP17 promoter-reporter construct was increased by Bt(2)cAMP and by overexpression of PKA or MKP-1. Cyclic AMP 123-127 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 66-72 12506119-7 2003 Finally, silencing MKP-1 expression using antisense oligonucleotides attenuated cAMP-stimulated hCYP17 expression, whereas silencing of ERK1/2 increased hCYP17 expression. Cyclic AMP 80-84 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 96-102 11956159-0 2002 Adrenocorticotropin/cyclic adenosine 3",5"-monophosphate-mediated transcription of the human CYP17 gene in the adrenal cortex is dependent on phosphatase activity. Cyclic AMP 20-56 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 93-98 12506119-8 2003 These findings demonstrate integral roles for MKP-1 and ERK1/2 via regulation of the phosphorylation state of steroidogenic factor-1 (SF-1) in mediating ACTH/cAMP-dependent transcription of hCYP17, thereby maintaining the balance between transcriptional activation and repression. Cyclic AMP 158-162 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 190-196 12530662-2 2002 We have identified three proteins interacting with the human CYP17 cAMP responsive sequence (CRS): steroidogenic factor 1 (SF-1), p54nrb, and polypyrimidine tract-binding protein-associated splicing factor (PSF). Cyclic AMP 67-71 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 61-66 12530662-3 2002 Nuclear extracts isolated from cAMP stimulated of H295R cells showed cAMP-inducible binding to the human CYP17 (hCYP17) CRS. Cyclic AMP 31-35 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 105-110 12530662-3 2002 Nuclear extracts isolated from cAMP stimulated of H295R cells showed cAMP-inducible binding to the human CYP17 (hCYP17) CRS. Cyclic AMP 31-35 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 112-118 12530662-3 2002 Nuclear extracts isolated from cAMP stimulated of H295R cells showed cAMP-inducible binding to the human CYP17 (hCYP17) CRS. Cyclic AMP 69-73 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 105-110 12530662-3 2002 Nuclear extracts isolated from cAMP stimulated of H295R cells showed cAMP-inducible binding to the human CYP17 (hCYP17) CRS. Cyclic AMP 69-73 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 112-118 12200237-3 2002 We have recently shown that phosphoprotein phosphatase (PP) activity is essential for cAMP-dependent transcription of the human CYP17 (hCYP17) gene in H295R adrenocortical cells. Cyclic AMP 86-90 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 128-133 12200237-3 2002 We have recently shown that phosphoprotein phosphatase (PP) activity is essential for cAMP-dependent transcription of the human CYP17 (hCYP17) gene in H295R adrenocortical cells. Cyclic AMP 86-90 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 135-141 11956159-5 2002 Further, both okadaic acid and peroxyvanadate attenuate cAMP-stimulated increases in endogenous hCYP17 mRNA expression and in hCYP17 promoter-reporter construct luciferase activity. Cyclic AMP 56-60 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 96-102 11897684-1 2002 The first 57 bp upstream of the transcription initiation site of the human CYP17 (hCYP17) gene are essential for both basal and cAMP-dependent transcription. Cyclic AMP 128-132 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 75-80 11897684-1 2002 The first 57 bp upstream of the transcription initiation site of the human CYP17 (hCYP17) gene are essential for both basal and cAMP-dependent transcription. Cyclic AMP 128-132 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 82-88 11897684-4 2002 (Bu)(2)cAMP treatment resulted in a cAMP-inducible increase in the binding intensity of only the upper complex and also activated hCYP17 gene transcription. Cyclic AMP 7-11 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 130-136 11897684-4 2002 (Bu)(2)cAMP treatment resulted in a cAMP-inducible increase in the binding intensity of only the upper complex and also activated hCYP17 gene transcription. Cyclic AMP 36-40 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 130-136 11956159-4 2002 Using inhibitors of serine/threonine phosphatase activity (okadaic acid) and phosphotyrosine phosphatase activity (peroxyvanadate), we can inhibit the cAMP-inducible binding of the steroidogenic factor-1 (SF-1), p54(nrb)/NonO, and polypyrimidine tract-binding protein-associated splicing factor (PSF) complex required for regulation of transcription to the promoter of hCYP17. Cyclic AMP 151-155 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 369-375 11956159-5 2002 Further, both okadaic acid and peroxyvanadate attenuate cAMP-stimulated increases in endogenous hCYP17 mRNA expression and in hCYP17 promoter-reporter construct luciferase activity. Cyclic AMP 56-60 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 126-132 11956159-7 2002 Our findings demonstrate that activation of protein phosphatase(s) is essential for cAMP-dependent transcription of hCYP17 in H295R cells and suggest a role for PKA in phosphatase activation, which leads to dephosphorylation of SF-1 and increased gene transcription. Cyclic AMP 84-88 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 116-122 11196414-1 2000 CYP17 gene transcription is activated by SF-1 binding to a cyclic AMP-responsive sequence within the promoter region of the gene, and its transcription is inhibited by COUP-TF binding to the sequence. Cyclic AMP 59-69 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 0-5 11463853-2 2001 Human P450c17 is expressed in a cAMP-responsive, cell-specific, developmentally programmed fashion, but little is known about its transcriptional regulation. Cyclic AMP 32-36 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 6-13 11196462-3 2000 Cyclic AMP (cAMP) caused human adrenocortical H295R cells to overexpress hCYP17 resulting in hypersecretion of cortisol. Cyclic AMP 12-16 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 73-79 9851803-2 1998 CYP17 gene transcription has recently been shown to be activated by SF-1 (steroidogenic factor-1) binding to a cyclic AMP-responsive sequence within the promoter region of the gene, and inhibited by COUP-TF binding to the sequence. Cyclic AMP 111-121 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 0-5 10919257-7 2000 A stable exogenous analog of cAMP, 8 Br-cAMP, mimicked LH"s effect on steroidogenesis and StAR and CYP17 gene expression and with insulin stimulated StAR mRNA and hnRNA accumulation synergistically. Cyclic AMP 29-33 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 99-104 10720067-8 2000 In search of the physiological agents involved in this process, the effect of cAMP was tested on activity and phosphorylation state of our mutant CYP17 proteins. Cyclic AMP 78-82 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 146-151 10852468-9 2000 The results of these studies document: 1) that basal and cAMP-dependent CYP17 gene transcription is increased in PCOS theca cells; 2) that there is differential regulation of promoters of genes required for steroidogenesis in PCOS theca cells; and 3) that passaged normal and PCOS theca cells provide a model system for studying tissue-specific regulation of genes encoding steroidogenic enzymes and identifying the molecular mechanisms involved in increased androgen production in PCOS. Cyclic AMP 57-61 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 72-77 9029728-9 1997 We have recently shown that P450c17 must be phosphorylated on serine and threonine residues by a cAMP-dependent protein kinase to acquire 17,20 lyase activity. Cyclic AMP 97-101 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 28-35 9390195-0 1997 The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17. Cyclic AMP 44-48 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 82-87 9390195-6 1997 5"-Deletion analyses of the reporter construct containing this Pbx-binding site showed approximately sixfold induction by coexpression of Pbx1 and PKA compared to the basal transcription, suggesting that Pbx1 binds the -250/-241 bp sequence and participates in cAMP-dependent regulation of the human CYP17 gene. Cyclic AMP 261-265 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 300-305 9024219-2 1997 Using deletional promoter/reporter constructions for the human P450scc and P450c17 genes, we identified the regions conferring basal and cAMP-induced transcription of these two genes in NCI-H295 human adrenal cells. Cyclic AMP 137-141 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 75-82 9024219-4 1997 In the P450c17 promoter, both basal and cAMP-responsive elements lie within the first upstream 63 bp, and a second basal element lies between -184 and -206 bp. Cyclic AMP 40-44 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 7-14 7479852-3 1995 We show that human P450c17 is phosphorylated on serine and threonine residues by a cAMP-dependent protein kinase. Cyclic AMP 83-87 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 19-26 8597483-5 1995 Agents that act by increasing intracellular calcium (angiotensin II and K+ ions) as well as protein kinase A pathway activators (ACTH, forskolin, and dibutyryl cAMP) individually increased the mRNA levels and activity of P450c17. Cyclic AMP 160-164 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 221-228 7811386-11 1994 Second-messenger cyclic adenosine monophosphate and adrenocorticotropin specifically increased the abundance of P450c17 mRNA levels in guinea pig adrenal cells. Cyclic AMP 17-47 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 112-119 22217838-3 1992 By deletion analysis of their upstream regions, the genes encoding the steroid hydroxylases P450c17, P450c21 and P450scc (CYP17, CYP21B and CYP11A, respectively) were found to contain unique cAMP-responsive sequences (CRSs). Cyclic AMP 191-195 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 92-99 22217838-3 1992 By deletion analysis of their upstream regions, the genes encoding the steroid hydroxylases P450c17, P450c21 and P450scc (CYP17, CYP21B and CYP11A, respectively) were found to contain unique cAMP-responsive sequences (CRSs). Cyclic AMP 191-195 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 122-127 3034954-9 1987 These results indicate that ACTH induces human fetal adrenal cells to accumulate mRNAs for both P450scc and P450c17; this effect of ACTH is probably mediated by cAMP. Cyclic AMP 161-165 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 108-115 3349907-7 1988 The abundances of P450scc and P450c17 mRNAs in cultured fetal testis cells rose 2.5-fold (p less than 0.01) and 9.2-fold (p less than 0.001), respectively, in response to 0.5 mM cAMP, but the abundance of IGF-II mRNA was not affected. Cyclic AMP 178-182 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 30-37 1311271-0 1992 Distinct biochemical mechanisms for cAMP-dependent transcription of CYP17 and CYP21. Cyclic AMP 36-40 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 68-73 1964490-0 1990 Tissue-specific, cyclic adenosine 3",5"-monophosphate-induced, and phorbol ester-repressed transcription from the human P450c17 promoter in mouse cells. Cyclic AMP 17-53 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 120-127 23415678-9 2013 Only the cAMP inducer forskolin induced CYP17 activity, while CYP19 was induced by four test compounds in the H295R assay. Cyclic AMP 9-13 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 40-45