PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8053691-1 1994 Holocarboxylase synthetase was purified 18,000-fold from bovine liver cytosol by a sequence of ammonium sulfate fractionation, alumina C gamma fractionation, DEAE-Sepharose CL-6B, EAH-Sepharose 4B, Sephacryl S-200 HR, Bio-Gel HTP, and Phenyl-Superose HR 5/5 chromatographies. sephacryl s 198-209 holocarboxylase synthetase Bos taurus 0-26